CN111700075B - Artificial aquilaria wood, aquilaria wood essential oil enrichment liquid, aquilaria wood soap and production method thereof - Google Patents

Artificial aquilaria wood, aquilaria wood essential oil enrichment liquid, aquilaria wood soap and production method thereof Download PDF

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CN111700075B
CN111700075B CN202010504525.4A CN202010504525A CN111700075B CN 111700075 B CN111700075 B CN 111700075B CN 202010504525 A CN202010504525 A CN 202010504525A CN 111700075 B CN111700075 B CN 111700075B
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agilawood
soap
artificial
culture medium
temperature
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CN111700075A (en
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黎妹
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Maoming Yufeng Chenxiang Creative Industry Co ltd
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Maoming Yufeng Chenxiang Creative Industry Co ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A01N63/32—Yeast
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005—Antimicrobial preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/10—Washing or bathing preparations
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B9/00—Essential oils; Perfumes
    • C11B9/02—Recovery or refining of essential oils from raw materials
    • C11B9/025—Recovery by solvent extraction
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B9/00—Essential oils; Perfumes
    • C11B9/02—Recovery or refining of essential oils from raw materials
    • C11B9/027—Recovery of volatiles by distillation or stripping
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D13/00—Making of soap or soap solutions in general; Apparatus therefor
    • C11D13/10—Mixing; Kneading
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D13/00—Making of soap or soap solutions in general; Apparatus therefor
    • C11D13/14—Shaping
    • C11D13/18—Shaping by extrusion or pressing
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/48—Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D9/00—Compositions of detergents based essentially on soap
    • C11D9/02—Compositions of detergents based essentially on soap on alkali or ammonium soaps
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D9/00—Compositions of detergents based essentially on soap
    • C11D9/04—Compositions of detergents based essentially on soap containing compounding ingredients other than soaps
    • C11D9/22—Organic compounds, e.g. vitamins
    • C11D9/38—Products in which the composition is not well defined
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D9/00—Compositions of detergents based essentially on soap
    • C11D9/04—Compositions of detergents based essentially on soap containing compounding ingredients other than soaps
    • C11D9/44—Perfumes; Colouring materials; Brightening agents ; Bleaching agents
    • C11D9/442—Perfumes
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

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Abstract

The invention relates to an artificial agilawood, agilawood essential oil enrichment liquid, agilawood soap and a production method thereof. The invention adopts a chemical stimulation-inoculation method to obtain the artificial agilawood position, and supercritical CO is adopted to carry out2The method comprises obtaining concentrated solution of lignum Aquilariae Resinatum essential oil rich in sesquiterpene and chromone compounds, adding Tremella extractive solution, soap base (such as fractionated coconut oil, oleum ricini, oleum Olivarum, and sodium hydroxide), quality conditioner, and pH conditioner to obtain lignum Aquilariae Resinatum soap with cleaning, moistening, antibacterial, antiinflammatory, tranquilizing, and tranquilizing effects. The preparation process of the invention does not produce waste materials and waste liquids which are difficult to degrade, has no influence on the environment, the product is faintly acid, is safe and non-irritant to skin, and can keep fragrance for a long time, and compared with the traditional agilawood, the invention has better killing effect on fungi, candida albicans, escherichia coli and staphylococcus aureus.

Description

Artificial aquilaria wood, aquilaria wood essential oil enrichment liquid, aquilaria wood soap and production method thereof
Technical Field
The invention relates to the field of plant planting, deep processing and daily chemicals, in particular to an artificial aquilaria wood, an aquilaria wood essential oil enrichment liquid, an aquilaria wood soap and a production method thereof.
Background
Aquilaria sinensis refers to a piece of lignum Aquilariae Resinatum which is a special resin produced by self-repairing of Aquilaria sinensis (lour.) Spreng of Thymelaeaceae after natural or artificial injury. Chenxiang can be used as a medicine for promoting qi circulation to relieve pain, warming middle energizer to arrest vomiting, and absorbing qi to relieve dyspnea. It is commonly used for chest and abdomen distention, stuffy pain, stomach cold, vomiting, hiccup, deficiency of the kidney, dyspnea with rapid and urgent respiration. The lignum Aquilariae Resinatum can be used as spice to enhance fragrance by burning, smearing or fumigating, and has effects of tranquilizing mind, relieving pressure, resisting bacteria and diminishing inflammation.
The natural generation of the agilawood needs decades or even decades, and the time is very long. Meanwhile, the agilawood is deficient in natural resources, and the wild aquilaria sinensis is almost eliminated. Therefore, the government encourages the farmers to plant agilawood trees and obtains agilawood by the artificial agilawood formation technology. At present, no unified agilawood producing technical standard exists in China, and agilawood quality evaluation indexes obtained by each farmer are different.
Perfumed soap is a washing product made up by using oil, alkali and adjuvant, and can be used for cleaning and maintaining skin, hair and clothes. With the improvement of living standard of people, more functions of the soap are developed, and besides the basic cleaning function, the soap with other functions such as sterilization, whitening, moistening, mosquito repelling and the like is also popular with people. People in the new era advocate 'nature, safety, green and efficiency', enrich the functionality of the soap, and pay attention to the effectiveness and safety of soap products.
In recent years, the agilawood soap prepared from the artificial agilawood is easy to crack and paste, has short fragrance retention time, dry and rough skin after use, skin allergy after use and the like, and is related to the quality safety and the subsequent production process of the artificial agilawood.
Disclosure of Invention
In order to solve the existing problems, the invention aims to provide the artificial aquilaria sinensis and the production method thereof, and the prepared artificial aquilaria sinensis has stronger sterilization capability.
The invention also aims to provide the agilawood essential oil enrichment solution and a production method thereof.
The invention also aims to provide the artificial heartleaf eaglewood soap and the production method thereof, wherein the artificial heartleaf eaglewood soap has the functions of cleaning and moistening, sterilizing and diminishing inflammation, and tranquilizing and allaying excitement, and is not easy to crack and paste.
The purpose of the invention is realized as follows: the production method of the artificial edgeworthia chrysantha is characterized by comprising the following steps:
(1) selecting 5-10 years old eaglewood trees with the diameter larger than 10 CM;
(2) drilling holes on the same side of a trunk, drilling a small hole which is inclined downwards at intervals of 40-60CM from top to bottom in the upwind direction by using a drilling tool, wherein the hole diameter is 1-3CM, and the drilling depth is 1/4-1/3 of the diameter of the aquilaria sinensis tree;
3) 100-300g of 1-3% formic acid bacterial liquid is injected into the drilled hole, the drilled hole is sealed by a plastic film, and the time for collecting the agilawood blocks is more than 6 months, wherein the formic acid bacterial liquid contains one or more than two of staphylococcus aureus, multi-resistant staphylococcus aureus, candida albicans or escherichia coli.
Preferably, the preparation method of the formic acid bacteria liquid comprises the following steps: respectively preparing bacterial suspensions of staphylococcus aureus, multi-resistant staphylococcus aureus, candida albicans or escherichia coli with the density of 5x106CFU/ml; one or more than two bacterial suspensions are taken, 1-4 parts of physiological saline is added to form a mixed solution in a five-fold amount, and finally, formic acid is added to prepare formic acid bacterial solution with the mass percentage of formic acid of 1-3%.
The culture of staphylococcus aureusThe culture medium is tryptone soy broth culture medium TSB culture medium, and is cultured at 37 deg.C for 24 hr, diluted with normal saline, and formulated into 5 × 106Bacterial suspension at CFU/ml density; the culture medium of multi-resistant Staphylococcus aureus is tryptone soy broth TSB culture medium, and is cultured at 37 deg.C for 24 hr, diluted with normal saline to 5 × 106Bacterial suspension at CFU/ml density; the Candida albicans culture medium is potato glucose agar PDA culture medium, and is cultured at 25 deg.C for 24 hr, diluted with normal saline, and made into 5 × 106Bacterial suspension at CFU/ml density; the Escherichia coli culture medium is conventional LB culture medium, cultured at 37 deg.C for 24 hr, diluted with normal saline to 5 × 106Bacterial suspension at CFU/ml density.
The artificial lignum Aquilariae Resinatum obtained by the above production method.
The production method of the agilawood essential oil enrichment solution is characterized by comprising the following steps of:
(1) extracting by pulverizing the above artificial lignum Aquilariae Resinatum into 40-80 mesh granules, and supercritical CO2The equipment is used for extracting lignum Aquilariae Resinatum granules at 40-65 deg.C under 20-35MPa and CO2The flow rate is 100-300L/h, the extraction time is set to be 90-240 minutes, and the agilawood extract is obtained;
(2) purifying and enriching the agilawood extract obtained in the step (1) by using molecular distillation equipment, setting the temperature of a water bath at 75-80 ℃, the vacuum degree of a vacuum system at 0.1-100Pa, the first-stage purification temperature at 80-100 ℃, the first-stage film scraping speed at 150-ion of 250r/min, the first-stage collection temperature at 10-50 ℃, the second-stage purification temperature at 100-ion of 130 ℃, the second-stage film scraping speed at 150-ion of 250r/min and the second-stage collection temperature at 10-50 ℃ to obtain the second-stage light component agilawood essential oil enriched liquid.
The agilawood essential oil enrichment solution prepared by the preparation method.
An agilawood soap is characterized in that: the formula comprises the following components in percentage by weight:
Figure BDA0002526060970000031
the tremella extract is prepared by the following steps:
s1, crushing the dried tremella, and sieving the crushed tremella with a 60-100-mesh sieve for later use;
s2, pre-soaking and weighing tremella powder into a container, and adding 30-60 times of deionized water to soak for 4-6 hours to obtain a tremella solution;
s3, ultrasonic extraction, namely placing the container filled with the soaked white fungus solution into ultrasonic extraction equipment, setting the parameters of the ultrasonic equipment to be 300-500W, and extracting for 20-60 minutes to obtain the white fungus extraction solution;
s4, centrifuging to transfer the S3 white fungus extraction solution into a centrifuge, setting the speed as 2000-4000r/min and the time as 3-15 minutes, and obtaining the supernatant which is the white fungus extraction solution.
The soap base is prepared from the following components in parts by weight of 1-4: 3:2 coconut oil, castor oil, olive oil and sodium hydroxide are prepared by saponification reaction; the quality conditioner is one or more than two of EDTA, vitamin E and glycerol. The pH conditioning agent is one or more than two of vitamin C, citric acid and L-lactic acid, and the addition amount of the pH conditioning agent is to adjust the pH value range of the soap base to 5-6.5.
The production method of the agilawood soap is characterized by comprising the following steps:
(1) kneading and weighing the soap base, the agilawood essential oil enrichment liquid, the tremella extract and the quality conditioner according to a ratio, adding the weighed materials into a conventional soap making kneader, kneading and stirring uniformly for 15-30 minutes at the stirring temperature of 30-40 ℃;
(2) grinding, namely adding the kneaded and uniformly stirred material into a grinder, wherein the thickness of the ground soap flakes is 0.2-0.4 mm;
(3) putting the ground soap flakes into a vacuum plodder by a vacuum layering machine, and performing vacuum layering, wherein the temperature of a plodder muzzle is 40 ℃, and the temperature of cooling water introduced into the plodder is 3-10 ℃;
(4) printing, namely putting the pressed strip soap body into a printer for printing, and controlling the temperature of a mould to be between-10 and-25 ℃ to obtain the artificial agilawood scented soap.
Hair brushObtaining artificial lignum Aquilariae Resinatum part by chemical stimulation-inoculation method, and performing supercritical CO extraction2The method comprises obtaining concentrated solution of lignum Aquilariae Resinatum essential oil rich in sesquiterpene and chromone compounds, adding Tremella extractive solution, soap base (such as fractionated coconut oil, oleum ricini, oleum Olivarum, and sodium hydroxide), quality conditioner, and pH conditioner to obtain lignum Aquilariae Resinatum soap with cleaning, moistening, antibacterial, antiinflammatory, tranquilizing, and tranquilizing effects. The preparation process of the invention does not produce waste materials and waste liquids which are difficult to degrade, has no influence on the environment, the product is faintly acid, is safe and non-irritant to skin, and can keep fragrance for a long time, and compared with the traditional agilawood, the invention has better killing effect on fungi, candida albicans, escherichia coli and staphylococcus aureus.
Detailed Description
The invention relates to a production method of artificial agilawood, which comprises the following steps:
(1) selecting 5-10 years old eaglewood trees with the diameter larger than 10 CM;
(2) drilling holes on the same side of a trunk, drilling a small hole which is inclined downwards at intervals of 40-60CM from top to bottom in the upwind direction by using a drilling tool, wherein the hole diameter is 1-3CM, and the drilling depth is 1/4-1/3 of the diameter of the aquilaria sinensis tree;
3) 100-300g of formic acid bacterial liquid diluted by normal saline and 1-3 percent is injected into the drilled hole, the drilled hole is sealed by a plastic film, and the time for collecting the lignum aquilariae resinatum wood block is more than 6 months, wherein the formic acid bacterial liquid contains one or more than two of staphylococcus aureus, multi-resistant staphylococcus aureus, candida albicans or escherichia coli.
The time for collecting the lignum Aquilariae Resinatum blocks is more than 6 months, preferably more than 10 months, and generally 10-12 months in view of production efficiency, at this time, the lignum Aquilariae Resinatum blocks have good quality.
Preferably, the preparation method of the formic acid bacteria liquid comprises the following steps: preferably, the preparation method of the formic acid bacteria liquid comprises the following steps: respectively preparing bacterial suspensions of staphylococcus aureus, multi-resistant staphylococcus aureus, candida albicans or escherichia coli with the density of 5x106CFU/ml; one or more than two bacterial suspensions are respectively added1-4 parts of normal saline constitutes mixed solution in five times, and finally formic acid is added to prepare formic acid bacterial solution with the mass percentage of 1-3 percent of formic acid.
Wherein the culture medium of Staphylococcus aureus is tryptone soy broth TSB culture medium, and is prepared by culturing at 37 deg.C for 24 hr, diluting with normal saline to 5 × 106Bacterial suspension at CFU/ml density; the culture medium of multi-resistant Staphylococcus aureus is tryptone soy broth TSB culture medium, and is cultured at 37 deg.C for 24 hr, diluted with normal saline to 5 × 106Bacterial suspension at CFU/ml density; the Candida albicans culture medium is potato glucose agar PDA culture medium, and is cultured at 25 deg.C for 24 hr, diluted with normal saline, and made into 5 × 106Bacterial suspension at CFU/ml density; the Escherichia coli culture medium is conventional LB culture medium, cultured at 37 deg.C for 24 hr, diluted with normal saline to 5 × 106Bacterial suspension at CFU/ml density.
The artificial lignum Aquilariae Resinatum obtained by the above production method.
A production method of agilawood essential oil enrichment liquid comprises the following steps:
(1) extracting by pulverizing the above artificial lignum Aquilariae Resinatum into granules of 40-80 mesh, and supercritical CO2The equipment is used for extracting lignum Aquilariae Resinatum granules at 40-65 deg.C under 20-35MPa and CO2The flow rate is 100-300L/h, the extraction time is set to be 90-240 minutes, and the agilawood extract is obtained;
(2) purifying and enriching the agilawood extract obtained in the step (1) by using molecular distillation equipment, setting the temperature of a water bath at 75-80 ℃, the vacuum degree of a vacuum system at 0.1-100Pa, the first-stage purification temperature at 80-100 ℃, the first-stage film scraping speed at 150-ion of 250r/min, the first-stage collection temperature at 10-50 ℃, the second-stage purification temperature at 100-ion of 130 ℃, the second-stage film scraping speed at 150-ion of 250r/min and the second-stage collection temperature at 10-50 ℃ to obtain the second-stage light component agilawood essential oil enriched liquid.
The agilawood essential oil enrichment solution prepared by the preparation method.
An agilawood soap is characterized in that: the formula comprises the following components in percentage by weight:
Figure BDA0002526060970000051
preferably, the tremella extract is prepared by the following steps:
s1, crushing the dried tremella, and sieving the crushed tremella with a 60-100-mesh sieve for later use;
s2, pre-soaking and weighing tremella powder into a container, and adding 30-60 times of deionized water to soak for 4-6 hours to obtain a tremella solution;
s3, ultrasonic extraction, namely placing the container filled with the soaked white fungus solution into ultrasonic extraction equipment, setting the parameters of the ultrasonic equipment to be 300-500W, and extracting for 20-60 minutes to obtain the white fungus extraction solution;
s4, centrifuging to transfer the S3 white fungus extraction solution into a centrifuge, setting the speed as 2000-4000r/min and the time as 3-15 minutes, and obtaining the supernatant which is the white fungus extraction solution.
Preferably, the soap base is prepared from the following components in a weight ratio of 1-4: 3:2 coconut oil, castor oil, olive oil and sodium hydroxide are prepared by saponification reaction. Preferably, the soap base is prepared by the following steps:
s1, respectively weighing the fractionated coconut oil, the castor oil and the olive oil according to the proportion, adding the materials into a heat-resistant container, heating the materials to 30-40 ℃ in a water bath heating mode, and uniformly stirring the materials;
s2, weighing deionized water 2.5-3.5 times (most preferably 3 times) the amount of sodium hydroxide, adding into an alkali-resistant glass container, slowly adding the calculated required sodium hydroxide with rapid stirring, and cooling to 35-40 ℃ after the sodium hydroxide is completely dissolved to obtain sodium hydroxide alkali liquor for later use;
the mass (m) of sodium hydroxide is calculated as follows:
the proportion of the fractionated coconut oil, the castor oil and the olive oil is A to B to C; the saponification values of fractionated coconut oil, castor oil and olive oil are 0.190, 0.129 and 0.134 respectively; the total mass of the fractionated coconut oil, the castor oil and the olive oil is M after mixing.
Sodium hydroxide mass (M) { a/(a + B + C) × 0.190+ B/(a + B + C) × 0.129+ C/(a + B + C) × 0.134} × M
S3, slowly adding the cooled alkali liquor of S2 into an S1 container with stirring, stirring for 30-40 minutes, completely saponifying the alkali liquor and the mixed oil of S1, and adding a pH conditioner to adjust the pH value to 5-6.5 to obtain the genuine soap.
Preferably, the quality conditioner is one or more than two selected from EDTA, vitamin E and glycerol; it is preferred. The pH conditioner is one or more selected from vitamin C, citric acid and L-lactic acid, and is prepared by adding appropriate amount of soap base and adjusting pH value of the soap base to 5-6.5.
The production method of the agilawood soap is characterized by comprising the following steps:
(1) kneading and weighing the soap base, the agilawood essential oil enrichment liquid, the tremella extract and the quality conditioner according to a ratio, adding the weighed materials into a conventional soap making kneader, kneading and stirring uniformly for 15-30 minutes at the stirring temperature of 30-40 ℃;
(2) grinding, namely adding the kneaded and uniformly stirred material into a grinder, wherein the thickness of the ground soap flakes is 0.2-0.4 mm;
(3) putting the ground soap flakes into a vacuum plodder by a vacuum layering machine, and performing vacuum layering, wherein the temperature of a plodder muzzle is 40 ℃, and the temperature of cooling water introduced into the plodder is 3-10 ℃;
(4) printing, namely putting the pressed strip soap body into a printer for printing, and controlling the temperature of a mould to be between-10 and-25 ℃ to obtain the artificial agilawood scented soap.
According to the invention, the artificial aquilaria wood with corresponding sterilization capability is obtained by artificially inoculating specific strains, and then the sterilization effective components are enriched by supercritical and molecular distillation, so that the agilawood essential oil enriched liquid with further enhanced sterilization capability is obtained. Meanwhile, the tremella is used as a quality modifier of the agilawood soap, so that the problems that the agilawood soap is easy to crack and the skin is dry and rough are solved.
Example 1
Firstly, a chemical stimulation-inoculation method is used for obtaining agilawood blocks, and the specific operation steps are as follows:
1) selecting 5-10 years old eaglewood trees with the diameter larger than 10 CM;
2) drilling holes on the same side of a trunk, drilling a small hole which is inclined downwards at intervals of 50CM from top to bottom in the upwind direction by using a drilling tool, wherein the hole diameter is 1.5CM, and the drilling depth is one third of the diameter of the aquilaria sinensis tree;
3) 200g of 2% formic acid bacterial liquid is injected into the drilled hole, the drilled hole is sealed by a plastic film, and the time for collecting the agilawood edgeworthia chrysantha wood block is 10 months, wherein the formic acid bacterial liquid contains staphylococcus aureus, multi-resistant staphylococcus aureus, candida albicans or escherichia coli.
Wherein the culture medium of Staphylococcus aureus is tryptone soy broth TSB culture medium, and is cultured at 37 deg.C for 24 hr, diluted with normal saline to make 5x106Bacterial suspension at CFU/ml density.
The culture medium of multi-resistant Staphylococcus aureus is tryptone soy broth TSB culture medium, and is cultured at 37 deg.C for 24 hr, diluted with normal saline to 5 × 106Bacterial suspension at CFU/ml density.
The Candida albicans culture medium is potato glucose agar PDA culture medium, and is cultured at 25 deg.C for 24 hr, diluted with normal saline, and made into 5 × 106Bacterial suspension at CFU/ml density.
The Escherichia coli culture medium is conventional LB culture medium, cultured at 37 deg.C for 24 hr, diluted with normal saline to 5 × 106Bacterial suspension at CFU/ml density.
Taking one part of each of the four bacterial liquids, adding 1 part of physiological saline to form a mixed solution in a five-fold amount, and adding formic acid to prepare formic acid bacterial liquid with the formic acid content of 2%.
Secondly, the agilawood essential oil enrichment solution is prepared by the following steps:
s1, smashing agilawood knot incense wood blocks with the age of 10 months obtained by a chemical stimulation-inoculation method, and smashing to agilawood particles of 40 meshes;
s2, extracting with supercritical CO2The equipment extracts lignum Aquilariae Resinatum granule at 60 deg.C under 28MPa with CO2The flow rate is 200L/h, and the extraction time is set to be 120 minutes to obtain an agilawood extract;
s3, purifying and enriching by using molecular distillation MSD-80II equipment, setting the temperature of a water bath at 75 ℃, the vacuum degree of a vacuum system at 1Pa, the first-stage purification temperature at 80 ℃, the first-stage film scraping speed at 220r/min, the first-stage collection temperature at 12 ℃, the second-stage purification temperature at 110 ℃, the second-stage film scraping speed at 220r/min and the second-stage collection temperature at 30 ℃, and obtaining a second-stage light component aquilaria wood essential oil enrichment solution;
thirdly, the agilawood soap is prepared by the following steps:
1. kneading and weighing the soap base, the agilawood essential oil enrichment solution, the tremella extract, the glycerol, the EDTA and the vitamin E according to a proportion, adding the weighed materials into a conventional soap making kneader, kneading and stirring uniformly, wherein the stirring time is 20 minutes, and the stirring temperature is 40 ℃;
2. grinding, namely adding the kneaded and uniformly stirred material into a grinder, wherein the thickness of the ground soap flakes is 0.3 mm;
3. putting the ground soap flakes into a vacuum plodder by a vacuum layering, and performing vacuum layering, wherein the temperature of a plodder muzzle is 40 ℃, and the temperature of cooling water introduced into the plodder is 5 ℃;
4. printing, namely putting the pressed strip soap body into a printer for printing, and controlling the temperature of a die to be-20 ℃ to obtain the artificial edgeworthia chrysantha perfumed soap.
The soap base is prepared by the following steps:
s1, respectively weighing fractionated coconut oil, castor oil and olive oil according to the ratio of 4:3:2, adding into a heat-resistant container, heating to 40 ℃ in a water bath heating mode, and uniformly stirring;
s2, weighing deionized water, adding the deionized water into an alkali-resistant glass container, slowly adding the calculated required sodium hydroxide with rapid stirring until the sodium hydroxide is completely dissolved and cooling to 40 ℃;
s3, slowly adding the cooled alkali liquor of S2 into an S1 container with stirring, stirring for 30 minutes, completely saponifying the alkali liquor and the mixed oil of S1, and adding a pH conditioner to adjust the pH to 6.5 to obtain the soap base.
The tremella extract is prepared by the following steps:
s1, crushing the dried tremella and then sieving the crushed tremella with a 100-mesh sieve for later use;
s2, pre-soaking and weighing tremella powder into a container, and adding 45 times of deionized water to soak the tremella powder for 6 hours to obtain a tremella solution;
s3, ultrasonic extraction, namely placing the container filled with the soaked white fungus solution into ultrasonic extraction equipment, setting the parameters of the ultrasonic equipment to be 400W, and extracting for 30 minutes to obtain a white fungus extraction solution;
and S4, centrifuging to transfer the S3 tremella fuciformis extract solution into a centrifuge, setting the speed to be 3000r/min and the time to be 5 minutes, and obtaining the supernatant, namely the tremella fuciformis extract.
In this example, the quality conditioner was EDTA, vitamin E and glycerin; the pH conditioning agent is L-lactic acid.
The raw materials in table 1 corresponding to examples 2-5 and comparative examples 5-6 were weighed in proportion and prepared according to the method of example 1, to obtain the artificial eaglewood soap of the present invention.
TABLE 1A formula table of artificial lignum Aquilariae Resinatum soap (unit is mass fraction)
Figure BDA0002526060970000081
Figure BDA0002526060970000091
Comparative example 1
An lignum Aquilariae Resinatum soap is commercially available.
Comparative example 2
The agilawood soap is different from the agilawood soap in example 1 in that the agilawood essential oil is directly prepared into the agilawood soap by adopting the agilawood extract obtained in the step S2 without purification and enrichment in the step S3.
Comparative example 3
An agilawood soap is different from the agilawood soap in example 1 in that agilawood essential oil is purified and enriched in the step S3, and is purified and enriched by using molecular distillation MSD-80II equipment, wherein the temperature of a water bath is set to be 70 ℃, the vacuum degree of a vacuum system is set to be 1Pa, the first-stage purification temperature is 75 ℃, the first-stage film scraping speed is 220r/min, the first-stage collection temperature is 10 ℃, the second-stage purification temperature is 90 ℃, the second-stage film scraping speed is 220r/min, and the first-stage collection temperature is 25 ℃ to obtain the second-stage light component agilawood essential oil enriched liquid.
Comparative example 4
An agilawood soap is different from the agilawood soap in example 1 in that agilawood essential oil is purified and enriched in the step S3, and is purified and enriched by using molecular distillation MSD-80II equipment, wherein the temperature of a water bath is set to be 85 ℃, the vacuum degree of a vacuum system is set to be 1Pa, the first-stage purification temperature is 110 ℃, the first-stage film scraping speed is 220r/min, the first-stage collection temperature is 15 ℃, the second-stage purification temperature is 150 ℃, the second-stage film scraping speed is 220r/min, and the second-stage collection temperature is 38 ℃ to obtain the second-stage light component agilawood essential oil enriched liquid.
Example 6
An agilawood soap is different from the soap in example 1 in that in the chemical stimulation-inoculation method, 1 part of staphylococcus aureus in step 3) is inoculated and mixed with 4 parts of normal saline, 2% formic acid is added, and the strains are all cultured by a culture medium before mixing.
Experimental example 1 sensory evaluation and measurement of physical and chemical Properties
Sensory evaluation and physicochemical property measurement were performed for examples 1 to 6 and comparative examples 1 to 6, respectively, as follows:
Figure BDA0002526060970000092
Figure BDA0002526060970000101
TABLE 2 sensory evaluation and measurement data of physicochemical Properties
Figure BDA0002526060970000102
Therefore, the artificial fragrant soap has clear pattern, square soap shape, uniform color, fragrant smell, no rancidity or bad smell, smoothness, no sand sense, no excessive removal of oil on the surface of skin after weak acid use, no dry and rough skin phenomenon, durability, difficult paste in water, good bubble effect and fine foam.
Experimental example 2 measurement of bacteriostatic Properties
The antibacterial performance of the samples of example 1, example 6 and comparative examples 1-4 is respectively determined, the concentration of the test strain is 10% and the action time is 20min according to the standard QB/T2738-2012, and the antibacterial data of the following examples 1 and comparative examples 1-5 are obtained:
TABLE 3 results of the bacteriostatic properties
Figure BDA0002526060970000111
As can be seen from the above table, the agilawood soap of example 1 has the best antibacterial performance, the commercially available agilawood soap of comparative example 1 has the worst antibacterial performance, and the comparative examples 2-4 have lower antibacterial performance compared with example 1 because the enrichment and purification steps of the agilawood essential oil are weakened to different degrees, which proves that the enrichment and purification steps of the agilawood essential oil have the effect of enriching antibacterial active ingredients, so that the prepared agilawood soap has better antibacterial performance, and the influence of too high or too low temperature on the enriched antibacterial active ingredients is exerted. Example 6 shows that the antibacterial performance against staphylococcus aureus is good, but the antibacterial performance against multi-resistant staphylococcus aureus, candida albicans and escherichia coli is reduced, which indicates that the composition of the bacteria liquid used in the inoculation step of the chemical stimulation-inoculation method influences the composition of the agilawood component generated by stress reaction to generate corresponding antibacterial performance.

Claims (9)

1. The production method of the artificial edgeworthia chrysantha is characterized by comprising the following steps:
(1) selecting 5-10 years old eaglewood trees with the diameter larger than 10 CM;
(2) drilling holes on the same side of a trunk, drilling a small hole which is inclined downwards at intervals of 40-60CM from top to bottom in the upwind direction by using a drilling tool, wherein the hole diameter is 1-3CM, and the drilling depth is 1/4-1/3 of the diameter of the aquilaria sinensis tree;
3) inoculating bacteria, injecting 100-300g of formic acid bacterial liquid with the formic acid mass percentage content of 1-3% into a drilled hole, encapsulating the drilled hole by using a plastic film, and collecting the agilawood blocks for more than 6 months, wherein the preparation method of the formic acid bacterial liquid comprises the following steps: respectively preparing bacterial suspensions of staphylococcus aureus, multi-resistant staphylococcus aureus, candida albicans or escherichia coli with the density of 5x106CFU/ml; taking one part of the staphylococcus aureus bacterial suspension, adding 4 parts of physiological saline to form a mixed solution, or taking one part of each of the four bacterial suspensions, and adding 1 part of physiological saline to form a mixed solution; and finally, adding formic acid to prepare formic acid bacterial liquid with the formic acid mass percentage content of 1-3%.
2. The method for producing artificial edgeworthia chrysantha according to claim 1, characterized in that: the culture medium of Staphylococcus aureus is tryptone soy broth TSB culture medium, and is cultured at 37 deg.C for 24 hr, diluted with normal saline, and formulated into 5X106Bacterial suspension at CFU/ml density; the culture medium of multi-resistant Staphylococcus aureus is tryptone soy broth TSB culture medium, and is cultured at 37 deg.C for 24 hr, diluted with normal saline to 5 × 106Bacterial suspension at CFU/ml density; the Candida albicans culture medium is potato glucose agar PDA culture medium, and is cultured at 25 deg.C for 24 hr, diluted with normal saline, and made into 5 × 106Bacterial suspension at CFU/ml density; the Escherichia coli culture medium is conventional LB culture medium, cultured at 37 deg.C for 24 hr, diluted with normal saline to 5 × 106Bacterial suspension at CFU/ml density.
3. The artificial edgeworthia chrysantha obtained by the production method of claim 1 or 2.
4. The production method of the agilawood essential oil enrichment solution is characterized by comprising the following steps of:
(1) extracting by pulverizing the wood blocks of the artificial lignum Aquilariae Resinatum of claim 3 into 40-80 mesh granulesBoundary CO2The equipment is used for extracting lignum Aquilariae Resinatum granules at 40-65 deg.C under 20-35MPa and CO2The flow rate is 100-300L/h, the extraction time is set to be 90-240 minutes, and the agilawood extract is obtained;
(2) purifying and enriching the agilawood extract obtained in the step (1) by using molecular distillation equipment, setting the temperature of a water bath at 75-80 ℃, the vacuum degree of a vacuum system at 0.1-100Pa, the first-stage purification temperature at 80-100 ℃, the first-stage film scraping speed at 150-ion of 250r/min, the first-stage collection temperature at 10-50 ℃, the second-stage purification temperature at 100-ion of 130 ℃, the second-stage film scraping speed at 150-ion of 250r/min and the second-stage collection temperature at 10-50 ℃ to obtain the second-stage light component agilawood essential oil enriched liquid.
5. The aquilaria essential oil enrichment solution prepared by the production method of claim 4.
6. An agilawood soap is characterized in that: the formula comprises the following components in percentage by weight:
Figure FDA0003055831650000021
7. the agilawood soap of claim 6, wherein: the tremella extract is prepared by the following steps:
s1, crushing the dried tremella, and sieving the crushed tremella with a 60-100-mesh sieve for later use;
s2, pre-soaking and weighing tremella powder into a container, and adding 30-60 times of deionized water to soak for 4-6 hours to obtain a tremella solution;
s3, ultrasonic extraction, namely placing the container filled with the soaked white fungus solution into ultrasonic extraction equipment, setting the parameters of the ultrasonic equipment to be 300-500W, and extracting for 20-60 minutes to obtain the white fungus extraction solution;
s4, centrifuging to transfer the S3 white fungus extraction solution into a centrifuge, setting the speed as 2000-4000r/min and the time as 3-15 minutes, and obtaining the supernatant which is the white fungus extraction solution.
8. The agilawood soap of claim 6, wherein: the soap base is prepared from the following components in parts by weight of 1-4: 3:2 coconut oil, castor oil, olive oil and sodium hydroxide are prepared by saponification reaction; the quality conditioner is one or more than two of EDTA, vitamin E and glycerol; the pH conditioning agent is one or more than two of vitamin C, citric acid and L-lactic acid, and the addition amount of the pH conditioning agent is to adjust the pH value range of the soap base to 5-6.5.
9. The method for producing the agilawood soap of claim 6, 7 or 8, characterized by comprising the steps of:
(1) kneading and weighing the soap base, the agilawood essential oil enrichment liquid, the tremella extract and the quality conditioner according to a ratio, adding the weighed materials into a conventional soap making kneader, kneading and stirring uniformly for 15-30 minutes at the stirring temperature of 30-40 ℃;
(2) grinding, namely adding the kneaded and uniformly stirred material into a grinder, wherein the thickness of the ground soap flakes is 0.2-0.4 mm;
(3) putting the ground soap flakes into a vacuum plodder by a vacuum layering machine, and performing vacuum layering, wherein the temperature of a plodder muzzle is 40 ℃, and the temperature of cooling water introduced into the plodder is 3-10 ℃;
(4) printing, namely putting the pressed strip soap body into a printer for printing, and controlling the temperature of a mould to be between-10 and-25 ℃ to obtain the artificial agilawood scented soap.
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