CN111694143A - Microscope and microscope integrated fluorescence imaging device - Google Patents
Microscope and microscope integrated fluorescence imaging device Download PDFInfo
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- CN111694143A CN111694143A CN202010696537.1A CN202010696537A CN111694143A CN 111694143 A CN111694143 A CN 111694143A CN 202010696537 A CN202010696537 A CN 202010696537A CN 111694143 A CN111694143 A CN 111694143A
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- 238000000799 fluorescence microscopy Methods 0.000 title claims abstract description 9
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- 239000003153 chemical reaction reagent Substances 0.000 description 3
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- G—PHYSICS
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- G02B21/00—Microscopes
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- G02B21/00—Microscopes
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- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
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- G—PHYSICS
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- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
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Abstract
The embodiment of the invention relates to an optical instrument, in particular to a microscope and a microscope integrated fluorescence imaging device, wherein the microscope comprises: the micro-lens is arranged in the shell, and the micro-lens, the rotary disc, the driving device and the optical filter are arranged in the shell; the housing includes: the side of the coaming, which is far away from the top plate, is an opening side, and one side of the top plate, which is opposite to the opening side, is provided with a CCD (charge coupled device); the microscope lens is coaxial with the CCD; the image side of the turntable is opposite to the image side of the microscope lens, the turntable is provided with a plurality of light holes around the center of the turntable, any light hole is used for being positioned on the same axis with the microscope lens when the turntable rotates for a preset angle, and the optical filter is arranged in any light hole; the driving device is connected with the turntable. Compared with the prior art, the medical staff can clearly obtain the images of the focus part and the non-focus part from the imaging device, and can perform the microscope operation according to the images, thereby further reducing the difficulty of the operation.
Description
Technical Field
The embodiment of the invention relates to an optical instrument, in particular to a microscope and a microscope integrated fluorescence imaging device.
Background
At present, medical fluorescence developer is used as a medical reagent for positioning tumor cancer cell focus parts in human organs, and is widely applied to various microscope operations, because the reagent can emit weak fluorescence after being combined with tumor cancer cells, development on an imaging device can be realized by means of a microscope for development, and therefore medical workers can position development areas in the operation process conveniently, the focus parts of tumors can be cut conveniently and quickly, and the operation difficulty is reduced. However, in practice, it has been found that, although the lesion site can be observed clearly and directly by the imaging device in the visualized image, the organ portion without lesion is not visualized by fluorescence with the reagent, and the brightness of the lesion site in the image displayed by the imaging device is obviously insufficient, so that the medical staff can only use the image as a reference during the operation, and cannot perform the operation directly according to the image.
In addition, since the microscope used for the development cannot be used for performing a conventional microscope operation, the microscope needs to be replaced when performing some conventional microscope operations, and the replacement procedure is troublesome.
Disclosure of Invention
An object of an embodiment of the present invention is to provide a microscope and a microscope-integrated fluorescence imaging apparatus, which enable medical staff to clearly obtain images of a focal site and a non-focal site from the microscope imaging apparatus, and to directly perform a microscope operation according to the obtained images, thereby further reducing the difficulty of the operation.
In order to achieve the above object, an embodiment of the present invention designs a microscope including:
a housing, comprising: the side of the coaming, which is far away from the top plate, is an unclosed opening side; an image chip CCD is arranged on one side, opposite to the opening side, of the top plate; the CCD is used for being electrically connected with the display terminal;
the microscope lens is arranged in the shell and is coaxial with the CCD; the object side of the micro lens is opposite to the opening side, and the image side of the micro lens is opposite to the top plate;
the rotating disc is rotatably arranged in the shell; the image sides of the turntable and the microscope lens are opposite to each other, a plurality of light holes are formed in the center of the turntable, and any light hole is used for being positioned on the same axis with the microscope lens when the turntable rotates for a preset angle;
the driving device is connected with the rotary disc; the driving device is used for driving the turntable to rotate;
and the optical filter is arranged in any light-transmitting hole.
In addition, an embodiment of the present invention relates to a microscope-integrated fluorescence imaging apparatus, including: the display terminal is electrically connected as described above;
the display terminal is used for acquiring image information uploaded by the CCD, the image information acquired by the optical filter is a first image, the image information not acquired by the optical filter is a second image, and the display terminal is also used for extracting a developing part in the first image and superposing the extracted developing part on the second image.
Compared with the prior art, because the microscope shell is internally provided with the microscope lens and the turntable, the shell is composed of the top plate and the coaming, one side of the coaming, which is far away from the top plate, is an opening side, one side of the top plate, which is opposite to the opening side, is provided with the CCD, the CCD and the microscope lens are positioned on the same axis, and the driving device is connected with the disk center of the turntable, the turntable can be driven to rotate, in addition, the turntable is provided with a plurality of light holes which are used for being positioned on the same axis with the microscope lens when the turntable rotates for a preset angle, and the light filter is arranged in any light hole, when the driving device drives the turntable, the light with specific wavelength of the developing part can pass through the light filter, thereby the CCD can image the developing part and send the image information to the display terminal, the image information is displayed as a first image by a display terminal, and a developed portion in the first image is extracted. Then, the driving device drives the rotating disc to rotate again until the light hole without the optical filter and the microscope lens are positioned on the same axis, at the moment, the CCD can image again and send the image information to the display terminal again, the display terminal displays the image information as a second image, and meanwhile, the developing part extracted from the first image is superposed on the second image, so that a clear organ image with a focus developing part is obtained. In addition, because at least one light hole is not provided with a light filter, the imaging device of the embodiment can also be suitable for common microscope operation, and the microscope device does not need to be replaced.
In addition, the driving device is arranged in the shell or outside the shell.
In addition, the driving device is a motor, and a main shaft of the motor is coaxially connected with the disk center of the rotating disk; or,
the driving device includes: the motor and the gear coaxially fixed with the main shaft of the motor are arranged, and a tooth-shaped surface meshed with the gear is arranged on one side of the rotary table, opposite to the top plate, around the circumferential direction of the rotary table.
In addition, the microscope further includes:
the light guide cover is arranged in the shell and is coaxial with the CCD; the CCD is positioned in the light guide cover;
one side of the light guide cover is a light outlet side connected with the top plate, the other side of the light guide cover is a light inlet side, and the light inlet side extends towards the direction of the microscope lens.
In addition, the internal diameter of light guide cover is followed the light-emitting side is towards the direction of advancing the light side grow gradually, the light guide cover is a toper structure.
In addition, the light guide cover is mutually separated from the rotary disc or is in sliding fit with the rotary disc.
In addition, the light guide cover is in sliding fit with the rotary table, an annular groove is formed in the light inlet side of the light guide cover, and a plurality of balls are arranged in the annular groove; the balls are mutually attached and are abutted against the rotary disc.
In addition, a groove wall on one side of the annular groove forms a plurality of first concave surfaces corresponding to the balls, and the other groove wall of the annular groove forms a plurality of second concave surfaces corresponding to the balls;
the first concave surfaces and the second concave surfaces are the same in number with the balls and only correspond to the balls, and the corresponding first concave surfaces and the corresponding second concave surfaces jointly form a rolling positioning area for positioning the balls.
In addition, the number of the light holes is larger than that of the optical filters, one optical filter is arranged in one light hole, and the wavelength ranges penetrated by light rays are different among the optical filters.
Drawings
FIG. 1 is a schematic structural view of a microscope with a driving device disposed on a top plate according to a first embodiment of the present invention;
FIG. 2 is a schematic structural view of a microscope with a driving device mounted on a fence according to a first embodiment of the present invention;
FIG. 3 is a schematic diagram illustrating the combination of an optical filter and a micro lens according to a first embodiment of the present invention;
FIG. 4 is a schematic view of the light guide cover and the turntable according to the first embodiment of the present invention;
FIG. 5 is a schematic cross-sectional view of a light guide cover along a radial direction according to a first embodiment of the present invention;
FIG. 6 is a schematic structural diagram of a micro lens according to a first embodiment of the present invention;
FIG. 7 is an exploded view of the objective lens assembly according to the first embodiment of the present invention;
FIG. 8 is an assembled view of the objective lens assembly according to the first embodiment of the present invention;
FIG. 9 is an enlarged view of a portion A of FIG. 7;
FIG. 10 is a schematic view of an objective lens assembly according to a first embodiment of the present invention, wherein the annular grooves are inclined planes combined with vertical planes;
FIG. 11 is a schematic view of the objective lens assembly according to the first embodiment of the present invention, wherein the annular ridge is a circular arc;
fig. 12 is a system block diagram of a microscope-integrated fluorescence imaging apparatus according to the second embodiment.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, embodiments of the present invention will be described in detail below with reference to the accompanying drawings. However, it will be appreciated by those of ordinary skill in the art that numerous technical details are set forth in order to provide a better understanding of the present application in various embodiments of the present invention. However, the technical solutions claimed in the claims of the present application can be implemented without these technical details and with various changes and modifications based on the following embodiments.
A first embodiment of the present invention relates to a microscope, as shown in fig. 1, including: the microscope comprises a shell 1, a microscope lens 2, a rotary table 3, a driving device 4 and an optical filter 5.
As shown in fig. 1, in the present embodiment, the housing 1 includes: the image display device comprises a top plate 11 and a baffle plate 12 arranged around the circumference of the top plate 11, wherein one side of the baffle plate 12 far away from the top plate 11 is an unclosed opening forming side 13, one side of the top plate 11 opposite to the opening side 13 is provided with an image chip CCD 6, and the CCD 6 is used for being electrically connected with a display terminal as shown in a combined mode in fig. 2.
In addition, as shown in fig. 1, in the present embodiment, the microlens 2 is provided inside the housing 1, and is provided coaxially with the CCD 6; the object side 24 of the microscope lens is opposite to the opening side 13 of the housing 1, and the image side 23 of the microscope lens 2 is opposite to the top plate 11 of the housing 1.
Further, as shown in fig. 1, in the present embodiment, the turret 3 is rotatably provided inside the housing 1, and the turret 3 and the image side 23 of the microscope lens 2 are opposed to each other. Moreover, the turntable 3 is provided with a plurality of light holes 31 around the center of the turntable, and any light hole 31 can be positioned on the same axis with the microscope lens 2 when the turntable 3 rotates a preset angle. And the corresponding filter 5 is installed and fixed in any of the light transmission holes 31.
Finally, as shown in fig. 1, a driving device 4 is connected to the turntable 3 and is configured to drive the turntable 3 to rotate.
In practical application, the driving device 4 can drive the rotating disc 3 to rotate until the light hole 31 with the optical filter 5 and the micro lens 2 are in the same axis, at this time, because the developed focus part can emit weak fluorescence, other light rays can be filtered by the optical filter 5, only the fluorescence with specific wavelength can pass through, so that the CCD 6 can image the developed part and send the image information to the display terminal, the display terminal displays the image information as a first image and extracts the developed part in the first image, for example, the developed part can be marked and extracted in a coloring mode. Then, the rotating disc 3 can be driven to rotate again by the driving device 4 until the light hole 31 without the optical filter 5 and the microscope lens 2 are in the same axis, at this time, the CCD 6 can image again and send the image information to the display terminal again, the display terminal displays the image information as a second image, and simultaneously superimposes the development part extracted from the first image on the second image, so that a clear organ image with a focus development part can be obtained. In addition, because at least one light hole is not provided with a light filter, the imaging device of the embodiment can also be suitable for common microscope operation, and the microscope device does not need to be replaced.
Specifically, in the present embodiment, as shown in fig. 3, the number of the light transmission holes 31 on the turntable 3 is larger than the number of the filters, and only one filter 5 is provided in one light transmission hole 31, and the wavelength ranges through which light passes are different between the filters.
In the present embodiment, as shown in fig. 1, the driving device 4 is provided in the housing 1, and the driving device 4 is a motor 41 provided on the top plate 11 in order to rotate the turntable 3, and a spindle 42 of the motor 41 is coaxially connected to the hub of the turntable 3. Alternatively, as shown in fig. 2, the driving device 4 includes: the motor 41 is arranged on the enclosing plate 12, the gear 43 is coaxially fixed with the main shaft 42 of the motor 41, the corresponding side of the rotary table 3 opposite to the top plate 11 is provided with a tooth-shaped surface 32 around the circumferential direction of the rotary table 3, and the tooth-shaped surface 32 is meshed with the gear 43, so that the rotary table 3 can be rotated by driving the gear 43 through the motor 41. It should be understood that the driving device 4 in the present embodiment is described only by way of example as being disposed inside the housing 1, but in practical applications, the driving device 4 may be disposed outside the housing 1, for example, when the driving device 4 is the motor 41, the motor 41 may be disposed on a side of the top plate 11 away from the opening side 13 of the enclosure 12, and a spindle of the motor 41 may penetrate through the top plate 11 to be connected to the hub of the turntable 3, so that the driving device 4 can drive the turntable 3.
Further, as shown in fig. 1, the microscope of the present embodiment preferably further includes: and a light guide cover 7 disposed in the housing 1, wherein the light guide cover 7 is disposed coaxially with the CCD 6. Meanwhile, the CCD 6 is located inside the light guide cover 7. Specifically, as shown in fig. 4, one side of the light guide cover 7 is a light exit side 71 connected to the top plate 11, the other side of the light guide cover 7 is a light entrance side 72, and the light entrance side 72 extends in the direction of the microlens 2. Therefore, light scattering can be avoided through the light guide cover 7, light can enter the CCD 6 in a concentrated mode, and the success rate and the definition of CCD 6 imaging are improved. In the present embodiment, as shown in fig. 4, the inner diameter of the light guide cover 7 gradually increases from the light exit side 71 to the light entrance side 72, so that the entire light guide cover 7 has a tapered structure, and light can enter the CCD 6 more intensively.
In addition, it is worth mentioning that, in order to avoid the light guide cover 7 affecting the rotation of the turntable 3, in the present embodiment, the light guide cover 7 may be spaced apart from the turntable 3. Of course, the light guide cover 7 and the turntable 3 may be in sliding fit to further increase the brightness of the light entering the CCD 6. Specifically, as shown in fig. 4 and 5, when the light guide cover 7 and the turntable 3 are in sliding fit, an annular groove 73 is formed in the light inlet side 72 of the light guide cover 7, a plurality of balls 8 are arranged in the annular groove 73, the balls 8 are attached to each other, and the balls 8 are abutted against the turntable 3.
It can be seen from this that, as shown in fig. 4, when the rotary plate 3 rotates, the balls 8 can freely roll in the annular groove 73 by the balls 8 disposed in the annular groove 73, so that the sliding fit between the rotary plate 3 and the light guide cover 7 is achieved, and the balls 8 are attached to each other, so that the light leakage phenomenon can be further prevented, and the amount of light entering the CCD 6 can be further increased.
Preferably, as shown in fig. 5, in the present embodiment, a plurality of first concave surfaces 7311 are formed on one groove wall 731 of the annular recess 73 corresponding to each ball 8, and a plurality of second concave surfaces 7321 are formed on the other groove wall 732 of the annular recess 73 corresponding to each ball 8. Moreover, the first concave surfaces 7311 and the second concave surfaces 7321 are the same in number and uniquely correspond to the balls 8, and the corresponding first concave surfaces 7311 and the second concave surfaces 7321 together form a rolling positioning area 735 for positioning the balls 8, so that the balls 8 can be positioned while interference between the balls 8 during rolling can be effectively avoided through the positioning areas 735, and the rolling performance of the balls 8 is further improved.
In addition, in the present embodiment, as shown in fig. 6, the microlens 2 includes: a lens barrel 21 and an objective lens group 22, as shown in fig. 7 and 8, the objective lens group 22 includes: at least a first lens 221 and a second lens 222 stacked on each other, the first lens 221 including: a first optically effective portion 2211 for image formation, and a first non-optically effective portion 2212 surrounding the first optically effective portion 2211. And the second lens 222 includes: a second optically effective portion 2221 for imaging, and a second non-optically effective portion 2222 surrounding the second optically effective portion 2221.
In addition, as shown in fig. 7 and 8, the first lens 221 further includes: an annular projection 2213, the annular projection 2213 is formed to project from the image side 22121 of the first non-optical effective portion 2212 in a direction away from the object side 22122, and the whole second lens 222 is located in the annular projection 2213. Wherein the annular projection 2213 includes: an inner side surface, an outer side surface 22132 opposite to the inner side surface, and the inner side surface is a first annular inclined surface 22131, and the first annular inclined surface 22131 is formed by gradually expanding and extending from the image side 22121 of the first non-optically effective portion 2212 toward the second lens element 222.
As shown in fig. 8, the second lens element 222 has a second annular inclined surface 22223 outside the second non-optical effective portion 2222, the second annular inclined surface 22223 is formed by gradually expanding and extending from the object side 22221 of the second non-optical effective portion 2222 toward the image side 22222, and the second annular inclined surface 22223 is engaged with the first annular inclined surface 22131.
As can be seen from the above, since the image side 22121 of the first non-optical effective portion 2212 of the first lens 221 protrudes away from the object side 22122 to form the annular protrusion 2213, the inner side of the annular protrusion 2213 is the first annular inclined surface 22131, and the first annular inclined surface 22131 is formed by gradually expanding and extending from the image side 22121 of the first non-optical effective portion 2212 toward the direction away from the object side 22122, and the second lens 222 is disposed in the annular protrusion 2213, and the outer side of the second non-optical effective portion 2222 of the second lens 222 is the second annular inclined surface 22223, and is engaged with the first annular inclined surface 22131 of the annular protrusion 2213, when the first lens 221 and the second lens 222 are stacked, the first annular inclined surface 22131 and the second annular inclined surface 22223 are engaged with each other, so as to ensure that the two lenses can be aligned with each other, and while facilitating the alignment, the alignment accuracy of the positioning between the first lens 221 and the second lens 222 can be improved, so as to improve the imaging quality of the final lens.
Note that, as shown in fig. 7 and 8, the outer side surface of the first non-optically-effective portion 2212 is a third annular inclined surface 22123, and the third annular inclined surface 22123 is formed by gradually expanding from the object side 22122 of the first non-optically-effective portion 2212 toward the image side 22121. Therefore, as shown in fig. 6, the inner wall (not shown) of the lens barrel 21 of the lens barrel may be designed to be an annular inclined surface corresponding to the third annular inclined surface 22123 of the first non-optical effective portion 2212, so that the annular inclined surface 22123 can be engaged with the inner wall of the lens barrel 21, when the objective lens assembly of this embodiment is assembled in the lens barrel 21, the third annular inclined surface 22123 can be engaged with the inner wall of the lens barrel 21, and the objective lens assembly of this embodiment can be centered with the lens barrel 21 automatically, so that the objective lens assembly of this embodiment and the lens barrel 21 can be coaxial.
Further, as shown in fig. 6, 7 and 8, in the present embodiment, in the first lens 221, the outer side surface 22132 of the annular projection 2213 is connected to the outer side surface 2212 of the first non-optically effective portion 2212, i.e. connected to the third annular inclined surface 22123, i.e. the outer side surface 22132 and the third annular inclined surface 22123 are coplanar, and the outer side surface 22132 of the annular projection 2213 can continue to extend along the inclined direction of the outer side surface of the first non-optically effective portion 2212, so that the outer side surface 22132 of the annular projection 2213 can be similarly engaged with the inner wall of the lens barrel 21, and therefore, when the number of lenses in the objective lens group is larger than two, as shown in fig. 4, the installation of multiple lenses can be realized by the annular projection 2213, i.e. all the remaining lenses of the first lens 221 can be disposed in the annular projection 2213, so as to realize the stacked arrangement between the lenses in the objective lens group.
In addition, in the first lens element 221, as shown in fig. 9, the image side 22121 of the first non-optical effective portion 2212 is provided with a plurality of annular lines 2214 from inside to outside in the optical axis direction. Through each annular line 2214, the area of the first non-optical effective part 2212 can be increased, meanwhile, stray light can be scattered irregularly, the possibility that the stray light penetrates through the non-optical effective part of the lens is reduced, the probability of occurrence of phenomena such as ghost, flare and the like is reduced, and the final imaging quality of the lens is improved.
In addition, in this embodiment, as shown in fig. 9, each annular ridge 2214 protrudes to the image side of the first non-optical effective portion 2212, and the annular ridges 2214 may be connected in sequence in this embodiment, so that each annular ridge 2214 is integrally formed as a continuous spiral structure, and the difficulty of stray light passing through the first non-optical effective portion 2212 of the first lens 221 may be increased by the spiral structure, thereby further improving the imaging quality of the lens.
Specifically, in the present embodiment, as shown in fig. 9, each annular ridge 2214 in the helical structure includes: a first inclined surface 22141, and a second inclined surface 22142 connected to the first inclined surface 22141. And the first inclined surface 22141 and the second inclined surface 22142 of each annular ridge 2214 are respectively connected with the first inclined surface 22141 or the second inclined surface 22142 of the adjacent annular ridge 2214. Meanwhile, the end of each annular line 2214 where the first inclined surface 22141 and the second inclined surface 22142 are connected is a sharp part 22143, so that the whole annular line 2214 is similar to a triangular structure, the intensity of incident light can be effectively weakened through the sharp part 22143, and the probability that stray light in the first non-optical effective part 2212 passes through the first lens 221 is greatly reduced. Preferably, the cross section of the annular stripe 22141 is an isosceles triangle, so that when the image side of the first non-optical effective portion 2212 of the first lens 221 scatters stray light, the stray light can be more uniformly scattered in all directions, thereby further improving the irregular scattering effect of the reflected stray light and reducing the energy of the stray light.
Of course, in practical applications, as shown in fig. 10, as an alternative, each annular ridge 2214 may also be formed by connecting an inclined surface 22144 and a vertical surface 22145. Alternatively, as shown in fig. 11, each annular ridge 2214 is integrally formed as an annular circular arc 22146. The annular ridge 2214 in this manner also greatly reduces the probability that the first non-optically effective portion 2212 will block stray light from passing through the first lens 221.
As shown in fig. 6, 7, and 8, the objective lens group 22 according to the present embodiment further includes: an annular light shielding sheet 224, the annular light shielding sheet 224 is disposed around the optical axis on the image side 22121 of the first non-optical effective portion 2212, so that the annular light shielding sheet 224 can be located between the first lens element 221 and the second lens element 222, and light rays which do not need to enter the objective lens group can be shielded by the annular light shielding sheet 224.
Meanwhile, in order to fix the annular light-shielding sheet 224, as shown in fig. 9, in the first lens 221, the first annular inclined surface 22131 is provided with an annular positioning groove 22133 around the optical axis direction, the annular light-shielding sheet 224 is embedded in the annular positioning groove 22133, and the annular light-shielding sheet 224 can be directly embedded in the annular positioning groove 22133, so that the annular light-shielding sheet 224 can be clamped and fixed.
A second embodiment of the present invention relates to a microscope-integrated fluorescence imaging apparatus, as shown in fig. 12, including: the microscope and the display terminal according to the first embodiment. The CCD 6 and the driving device 4 of the microscope are both electrically connected with the display terminal.
As shown in fig. 1, the display terminal is used to acquire image information uploaded by CCD 6. The image information acquired through the optical filter 5 is a first image, and the image information not acquired through the optical filter is a second image. The display terminal is also used for extracting the developing part in the first image and superposing the extracted developing part on the second image.
As can be seen from the above, in the practical application process, the driving device 4 can drive the rotating disc 3 to rotate until the light hole 31 with the optical filter 5 installed thereon and the microscope lens 2 are located on the same axis, at this time, since the developed lesion site emits weak fluorescence, other light can be filtered by the optical filter 5, and only fluorescence with a specific wavelength can pass through, so that the CCD 6 can image the developed portion and send the image information to the display terminal, the display terminal displays the image information as a first image, and extracts the developed portion in the first image, for example, the developed portion can be marked and extracted in a coloring manner. Then, the rotating disc 3 can be driven to rotate again by the driving device 4 until the light hole 31 without the optical filter 5 and the microscope lens 2 are in the same axis, at this time, the CCD 6 can image again and send the image information to the display terminal again, the display terminal displays the image information as a second image, and simultaneously superimposes the development part extracted from the first image on the second image, so that a clear organ image with a focus development part can be obtained. In addition, because at least one light hole is not provided with a light filter, the imaging device of the embodiment can also be suitable for common microscope operation, and the microscope device does not need to be replaced.
It will be understood by those of ordinary skill in the art that the foregoing embodiments are specific examples for carrying out the invention, and that various changes in form and details may be made therein without departing from the spirit and scope of the invention in practice.
Claims (10)
1. A microscope, comprising:
a housing, comprising: the side of the coaming, which is far away from the top plate, is an unclosed opening side; an image chip CCD is arranged on one side, opposite to the opening side, of the top plate; the CCD is used for being electrically connected with the display terminal;
the microscope lens is arranged in the shell and is coaxial with the CCD; the object side of the micro lens is opposite to the opening side, and the image side of the micro lens is opposite to the top plate;
the rotating disc is rotatably arranged in the shell; the image sides of the turntable and the microscope lens are opposite to each other, a plurality of light holes are formed in the center of the turntable, and any light hole is used for being positioned on the same axis with the microscope lens when the turntable rotates for a preset angle;
the driving device is connected with the rotary disc; the driving device is used for driving the turntable to rotate;
and the optical filter is arranged in any light-transmitting hole.
2. The microscope of claim 1, wherein the drive device is disposed within the housing or outside the housing.
3. The microscope of claim 2, wherein the driving device is a motor, and a spindle of the motor is coaxially connected with a disk center of the turntable; or,
the driving device includes: the motor and the gear coaxially fixed with the main shaft of the motor are arranged, and a tooth-shaped surface meshed with the gear is arranged on one side of the rotary table, opposite to the top plate, around the circumferential direction of the rotary table.
4. The microscope of claim 1, further comprising:
the light guide cover is arranged in the shell and is coaxial with the CCD; the CCD is positioned in the light guide cover;
one side of the light guide cover is a light outlet side connected with the top plate, the other side of the light guide cover is a light inlet side, and the light inlet side extends towards the direction of the microscope lens.
5. The microscope of claim 4, wherein the light guide cover has an inner diameter that gradually increases from the light exit side to the light entrance side, and the light guide cover has a tapered structure.
6. The microscope of claim 4, wherein the light guide is spaced from the carousel or is a sliding fit with the carousel.
7. The microscope of claim 6, wherein the light guide cover is slidably engaged with the turntable, an annular groove is formed on the light inlet side of the light guide cover, and a plurality of balls are arranged in the annular groove; the balls are mutually attached and are abutted against the rotary disc.
8. The microscope of claim 7, wherein one groove wall of the annular groove forms a plurality of first concave surfaces corresponding to the balls, and the other groove wall of the annular groove forms a plurality of second concave surfaces corresponding to the balls;
the first concave surfaces and the second concave surfaces are the same in number with the balls and only correspond to the balls, and the corresponding first concave surfaces and the corresponding second concave surfaces jointly form a rolling positioning area for positioning the balls.
9. The microscope of claim 1, wherein the number of the light-transmitting holes is greater than the number of the filters, and one of the filters is disposed in one of the light-transmitting holes, and the wavelength ranges of the light transmitted through the filters are different.
10. A microscope integrated fluorescence imaging device, comprising: the microscope, display terminal of any one of claims 1-9; the CCD and the driving device of the microscope are electrically connected with the display terminal;
the display terminal is used for acquiring image information uploaded by the CCD, the image information acquired by the optical filter is a first image, the image information not acquired by the optical filter is a second image, and the display terminal is also used for extracting a developing part in the first image and superposing the extracted developing part on the second image.
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