CN111693196A - Live embryo surface zona pellucida tension detection device - Google Patents
Live embryo surface zona pellucida tension detection device Download PDFInfo
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- CN111693196A CN111693196A CN202010546928.5A CN202010546928A CN111693196A CN 111693196 A CN111693196 A CN 111693196A CN 202010546928 A CN202010546928 A CN 202010546928A CN 111693196 A CN111693196 A CN 111693196A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01L—MEASURING FORCE, STRESS, TORQUE, WORK, MECHANICAL POWER, MECHANICAL EFFICIENCY, OR FLUID PRESSURE
- G01L5/00—Apparatus for, or methods of, measuring force, work, mechanical power, or torque, specially adapted for specific purposes
- G01L5/04—Apparatus for, or methods of, measuring force, work, mechanical power, or torque, specially adapted for specific purposes for measuring tension in flexible members, e.g. ropes, cables, wires, threads, belts or bands
Abstract
The invention discloses a live embryo surface transparent belt tension detection device, which comprises an inverted microscope, a pressure sensor fixedly connected with a first operating arm of the inverted microscope, an extrusion needle fixedly connected with the other end of the pressure sensor and used for extruding embryos, a fixing needle fixedly connected with a second operating arm of the inverted microscope, and a micromanipulation dish positioned on an objective table of the inverted microscope, wherein the pressure sensor is electrically connected with a computer through a data line, and transmits detected data to the computer for storage. The combined action of the extrusion needle, the fixing needle and the baffle plate enables the zona pellucida on the surface of the live embryo to slightly deform, the live embryo cannot be damaged, and finally, the tension coefficient of the live embryo is calculated, so that an embryologist can judge the activity of the live embryo together by using the data and the indexes commonly used for embryo judgment.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to a live embryo surface zona pellucida tension detection device.
Background
The conventional test tube infant is called as assisted reproduction technology in the industry, one traditional assisted reproduction technology comprises in vitro fertilization, when an ovum of a patient is taken out through operation after ovulation promotion, the ovum is combined with sperms of a husband in vitro, an embryo can be selectively transplanted back to a mother body to complete an assisted conception process after being cultured for 3-6 days, research data shows that if the patient is older than 35 years, the tension of a zona pellucida on the surface layer of the embryo of the patient becomes too large (commonly called zona pellucida is too hard), so that the embryo is difficult to hatch in the mother body, auxiliary hatching needs to be carried out, a laser method is popular at present, a laser module is arranged on a microscope, an embryo image is observed on a computer screen, zona pellucida emission of the embryo is aimed, the zona pellucida is punched through and then transplanted back to the mother body, but embryo punching by using laser can cause certain damage to the embryo, and, some adverse chemical changes may be caused, and in addition, if the embryo is frozen above 35 years old or if the embryo is frozen, the embryo may be cut once as a basis for assisted hatching, and people over 35 years old may have a low surface tension of the embryo, the embryo is soft and does not need assisted hatching, while people under 35 years old may have a non-ideal, hard and even assisted hatching condition of the zona pellucida.
There is also a case of freezing embryo, some people will not transfer the embryo back to the mother immediately after completing the embryo culture process, the embryo will be frozen, when one week, one month, one year or several years later, the patient will return to the hospital to recover the frozen embryo, the frozen embryo will be cured because of being soaked by the freezing liquid, so the auxiliary hatching technique is also applied, the embryo transparent belt is punched, and finally the embryo is transplanted back to the mother.
The hardness of the embryo which is not frozen is different, the frozen embryo can not be hardened at all, and the hardness of the embryo can be influenced by some chemical additives, so that an embryo surface tension measuring instrument is needed to measure the hardness of the embryo, a specific embryo surface tension value is given, the quality of the embryo can be conveniently evaluated by an embryologist together with some indexes commonly used for embryo judgment, such as cell number, uniformity, fragment quantity and the like, the embryo surface tension measuring instrument is used for judging the necessity of auxiliary hatching, and the embryo surface tension measuring instrument can be used for researching the influence of the added chemical substances and the freezing process on the surface tension of the embryo transparent belt.
Therefore, it is a problem to be studied to provide a device for detecting the tension of the zona pellucida on the surface of a living embryo, which is convenient to operate and can accurately measure.
Disclosure of Invention
In view of the above-mentioned shortcomings of the prior art, the present invention provides a device for detecting the tension of zona pellucida on the surface of a living embryo.
The purpose of the invention is realized as follows:
the utility model provides a live embryo surface zona pellucida tension detection device, includes the inverted microscope, still include with inverted microscope's first operation arm fixed connection's pressure sensor, with pressure sensor's other end fixed connection and be used for squeezing the embryo extrusion needle, with inverted microscope's second operation arm fixed connection be used for the supplementary fixed needle that makes the embryo fixed, be located the micromanipulation dish on inverted microscope's objective table, pressure sensor passes through data line and computer electrical connection, and pressure sensor transmits the data that detect for the computer and be used for the save, the camera record compression process on the inverted microscope.
The extrusion needle includes the connecting rod with pressure sensor's other end fixed connection, with the other end fixed connection's of connecting rod extrusion head, and the extrusion head is for being in the flat board of horizontal direction, and the contained angle between extrusion head and the connecting rod is 20~ 35.
The length of connecting rod is 55~60mm, the diameter of connecting rod is 1~2mm, the length of extrusion head in left right direction is 1~2mm, and the distance of extrusion head in front and back direction is 15~20um, and the thickness of extrusion head in vertical direction is 25~30 um.
The micromanipulation ware includes ascending holding tank of opening, be located the central point of holding tank and be in the separation blade of vertical direction, and the left surface and the right flank of separation blade are the plane.
The fixing needle is characterized in that a first through hole is formed in the needle head portion of the fixing needle and arranged along the length direction of the fixing needle, a second through hole is formed in the side face of the fixing needle and communicated with the first through hole, and the second through hole is connected with a microinjection instrument and used for applying negative pressure to the needle head of the fixing needle to adsorb embryos.
Has the positive and beneficial effects that: the invention adds a pressure sensor on the operating arm of the existing inverted microscope, which is convenient for collecting and researching the mechanical data of the transparent belt on the surface of the tested live embryo, the baffle plate in the micromanipulation dish is arranged to provide a supporting surface for the live embryo, the combined action of the extrusion needle and the baffle plate leads the live embryo to generate certain deformation, the corresponding image data of the embryo deformation is recorded by the camera, and is transmitted to a computer to predict the tension coefficient, so that the embryologist can conveniently judge the common indexes of the embryo judgment such as the number of cells, the uniformity, the quantity of fragments and the like by using the data and some embryos, and provides reference data for the research of the live embryo, the device can also assist the research of using chemical substances or the research of the freezing technology for the embryo, after the initial embryo is measured, different chemical substances or the freezing technology is applied to the embryo and then the device is used for, judging the influence of different chemical substances or freezing technology on the tension of the zona pellucida on the surface of the embryo.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the construction of a micromanipulation dish according to the present invention;
FIG. 3 is a cross-sectional view taken along the line A-A;
FIG. 4 is a schematic view of the structure of the extrusion needle of the present invention;
FIG. 5 is an enlarged schematic view of part B;
FIG. 6 is a schematic view of the structure of the C-direction extrusion head;
FIG. 7 is a schematic view of the structure of the fixing pin of the present invention;
FIG. 8 is a schematic diagram of the structure of the extrusion needle and the fixing needle for extruding embryos;
FIG. 9 is a schematic diagram of a simulation experiment;
FIG. 10 is a graph of compression distance versus pressure;
in the figure, the following steps are carried out: the microscope comprises an inverted microscope 1, a base 2, a microscope arm 3, a condenser 4, an objective lens 5, an objective table 6, a horizontal position adjusting rod 7, a first supporting arm 8, a first operating arm 9, a pressure sensor 10, an extrusion needle 11, a first rocker 12, a first signal line 13A, a second signal line 13B, a micromanipulation dish 14, a fixing needle 15, a second operating arm 16, a second rocker 17, a microinjection instrument 18, a second supporting arm 19, a blocking piece 20, a containing groove 21, a connecting rod 22, an extrusion head 23, a first through hole 24 and a second through hole 25.
Detailed Description
The invention is further illustrated by the following figures and examples.
Example 1
As shown in figure 1, a tension detection device for a transparent belt on the surface of a live embryo comprises an inverted microscope 1, wherein the inverted microscope 1 adopts a Nikon inverted microscope with the model of Ti-E/Ti-U/Ti-S, the inverted microscope 1 is provided with an Eppendorf microscopic operation set, the inverted microscope 1 comprises a base 2, a lens arm 3 fixedly connected with the upper surface of the base 2, a condenser 4 fixedly connected with the top of the lens arm 3, an objective lens 5 positioned on the upper surface of the base 2, a first operation arm 9 connected with the lens arm 3 through a first supporting arm 8, a second operation arm 16 connected with the lens arm 3 through a second supporting arm 19, the first operation arm 9 connected with a first rocker 12 through a first signal line 13A, the first operation arm 9 can move in the horizontal direction and the vertical direction by operating the first rocker 12, the second operation arm 16 connected with a second rocker 17 through a second signal line 13B, the second operating arm 16 can move in the horizontal direction and the vertical direction by operating the second rocker 17, the objective table 6 is fixedly connected with the microscope arm 3, the lower surface of the objective table 6 is provided with a horizontal position adjusting rod 7, the device also comprises a pressure sensor 10 fixedly connected with the first operating arm 9 of the inverted microscope 1, a fixing needle 15 fixedly connected with the second operating arm 16, a squeezing needle 11 fixedly connected with the other end of the pressure sensor 10 and used for squeezing embryos, and a micromanipulation dish 14 positioned on the objective table 6 of the inverted microscope 1, wherein the pressure sensor 10 is electrically connected with a computer through a data line, the pressure sensor 10 can be used for measuring submicron Newton level force, the pressure sensor 10 transmits the detected data to the computer for storage, the pressure sensor 10 is convenient for detecting the squeezing force of the squeezing needle 11 on live embryos, thereby helping to collect the data of the tension of the transparent belt on the surface of the live embryos, and the measured mechanical data are transmitted to a computer through a data line, the data are stored and are studied in the next step, and the whole embryo extrusion process can be recorded by a camera on the inverted microscope 1.
As shown in fig. 4, 5 and 6, the extrusion needle 11 includes a connecting rod 22 fixedly connected to the other end of the pressure sensor 10, and an extrusion head 23 fixedly connected to the other end of the connecting rod 22, the extrusion head 23 and the connecting rod 22 are integrally formed, the extrusion head 23 and the connecting rod 22 are made of organic glass, the extrusion head 23 is a flat plate in the vertical direction, that is, the front side, the rear side and the left side of the extrusion head 23 are all planes, and an included angle between the extrusion head 23 and the connecting rod 22 is 20 °. The length that connecting rod 22 was is 55mm, connecting rod 22's diameter is 1mm, extrusion head 23 is 1mm at the length of left right direction, and extrusion head 23 is 15um at the distance of front and back direction, and extrusion head 23 is 25um at the thickness of vertical direction.
As shown in fig. 2 and 3, the micro-manipulation vessel 14 includes an accommodating groove 21 with an upward opening, and a blocking piece 20 located at the center of the accommodating groove 21 and in the vertical direction, the left side surface and the right side surface of the blocking piece 20 are both flat surfaces, the height of the blocking piece 20 is smaller than the depth of the accommodating groove 21, and the material of the blocking piece 20 is the same as that of the micro-manipulation vessel 14.
As shown in fig. 7 and 8, a first through hole 24 is formed in a needle head portion of the fixing needle 15, the first through hole 24 is arranged along a length direction of the fixing needle, a second through hole 25 is formed in a side surface of the fixing needle 15, the second through hole 25 is communicated with the first through hole 24, the second through hole 25 is connected with the microinjection instrument 18 and used for applying negative pressure to the needle head of the fixing needle 15 to adsorb embryos, central axes of the first through hole 24 and the second through hole 25 are perpendicular, the fixing needle 15 is made of organic glass, a suction inlet of the microinjection instrument 18 is communicated with a suction outlet of the second through hole 25, and the microinjection instrument 18 applies negative pressure to the first through hole 24 (suction inlet) on the needle head of the fixing needle 15 through sucking suction media (air or mineral oil), so that the needle head of the fixing needle 15 adsorbs the embryos, migration of the embryos is prevented, and the embryos are kept stable when being squeezed.
When in use, the pressure sensor 10 is fixedly connected with the first operating arm 9 through a bolt assembly, the extrusion needle 11 is fixedly connected with the pressure sensor 10 through a connecting piece, one end of the fixing needle 15 close to the second through hole 25 is fixedly connected with the second operating arm 16 through a bolt assembly, the inverted microscope 1 is opened, a live embryo which is being cultured and a culture solution thereof are placed in the micromanipulation dish 14, then the micromanipulation dish 14 is placed on the objective table 6, the position of the objective table 6 is adjusted, the first operating arm 9 and the second operating arm 16 are adjusted to working positions, the position of the second operating arm 16 is controlled by operating the second rocker 17, the fixing needle 15 is moved beside the embryo in the field of view of the inverted microscope 1, so that the opening of the first through hole 24 is close to the embryo, the microinjector 18 is operated, so that the embryo is sucked on the fixing needle 15 by the negative pressure in the first through hole 24, then operating the second rocker 17 to move the embryo to the front of the baffle 20, making the edge of the embryo just contact with the baffle 20, controlling the moving position of the first operating arm 12 to make the rear part of the side surface of the extrusion head 23 align with the transparent belt on the surface of the live embryo and extrude the embryo, the extrusion degree is set to the maximum degree which can not damage the embryo, at this time, the lower surface of the extrusion head 23 is parallel to the bottom of the containing groove 21 and is slightly suspended, at the same time, the other side of the live embryo is contacted with the front surface of the baffle 20, at this time, the needle head of the fixed needle 15 is positioned on the left side surface of the embryo to assist the extrusion head 23 and prevent the embryo from moving, the acting force of the extrusion head 23 on the transparent belt on the surface of the live embryo is transmitted to the pressure sensor 10 through the connecting rod 22, when the extrusion head 23 releases the embryo at the same set distance step by step, the pressure sensor transmits the mechanical signal data detected when the compression distance changes, meanwhile, videos of the whole compression process are also recorded and stored in a computer, when the fact that the extrusion head 23 is separated from the cell is found through the inverted microscope 1 or the stress displayed by the computer is 0, the operation is finished, the collection is stopped, the tension coefficient is obtained through operation in the computer, the tension coefficient is used for estimating the hardness of the embryo, and the effect of fine measurement of the embryo tension is achieved. The whole process does not involve temperature and chemical changes, only slightly compresses physically, and has little or no influence on the embryo.
Example 2
As shown in figure 1, a tension detection device for a transparent belt on the surface of a live embryo comprises an inverted microscope 1, wherein the inverted microscope 1 adopts a Nikon inverted microscope with the model of Ti-E/Ti-U/Ti-S, the inverted microscope 1 is provided with an Eppendorf microscopic operation set, the inverted microscope 1 comprises a base 2, a lens arm 3 fixedly connected with the upper surface of the base 2, a condenser 4 fixedly connected with the top of the lens arm 3, an objective lens 5 positioned on the upper surface of the base 2, a first operation arm 9 connected with the lens arm 3 through a first supporting arm 8, a second operation arm 16 connected with the lens arm 3 through a second supporting arm 19, the first operation arm 9 connected with a first rocker 12 through a first signal line 13A, the first operation arm 9 can move in the horizontal direction and the vertical direction by operating the first rocker 12, the second operation arm 16 connected with a second rocker 17 through a second signal line 13B, the second operating arm 16 can move in the horizontal direction and the vertical direction by operating the second rocker 17, the objective table 6 is fixedly connected with the microscope arm 3, the lower surface of the objective table 6 is provided with a horizontal position adjusting rod 7, the device also comprises a pressure sensor 10 fixedly connected with the first operating arm 9 of the inverted microscope 1, a fixing needle 15 fixedly connected with the second operating arm 16, a squeezing needle 11 fixedly connected with the other end of the pressure sensor 10 and used for squeezing embryos, and a micromanipulation dish 14 positioned on the objective table 6 of the inverted microscope 1, wherein the pressure sensor 10 is electrically connected with a computer through a data line, the pressure sensor 10 can be used for measuring submicron Newton level force, the pressure sensor 10 transmits the detected data to the computer for storage, the pressure sensor 10 is convenient for detecting the squeezing force of the squeezing needle 11 on live embryos, thereby helping to collect the data of the tension of the transparent belt on the surface of the live embryos, and the measured mechanical data are transmitted to a computer through a data line, the data are stored and are studied in the next step, and the whole embryo extrusion process can be recorded by a camera on the inverted microscope 1.
As shown in fig. 4, 5 and 6, the extrusion needle 11 includes a connecting rod 22 fixedly connected to the other end of the pressure sensor 10, and an extrusion head 23 fixedly connected to the other end of the connecting rod 22, the extrusion head 23 and the connecting rod 22 are integrally formed, the extrusion head 23 and the connecting rod 22 are made of medical stainless steel, the extrusion head 23 is a flat plate in the vertical direction, that is, the front side, the back side and the left side of the extrusion head 23 are all flat, and the included angle between the extrusion head 23 and the connecting rod 22 is 35 °. The length that connecting rod 22 was is 60mm, connecting rod 22's diameter is 2mm, extrusion head 23 is 2mm at the length of left right direction, and extrusion head 23 is 20um at the distance of front and back direction, and extrusion head 23 is 30um at the thickness of vertical direction.
As shown in fig. 2 and 3, the micro-manipulation vessel 14 includes an accommodating groove 21 with an upward opening, and a blocking piece 20 located at the center of the accommodating groove 21 and in the vertical direction, the left side surface and the right side surface of the blocking piece 20 are both flat surfaces, the height of the blocking piece 20 is smaller than the depth of the accommodating groove 21, and the material of the blocking piece 20 is the same as that of the micro-manipulation vessel 14.
As shown in fig. 7 and 8, a first through hole 24 is formed in a needle head portion of the fixing needle 15, the first through hole 24 is arranged along a length direction of the fixing needle, a second through hole 25 is formed in a side surface of the fixing needle 15, the second through hole 25 is communicated with the first through hole 24, the second through hole 25 is connected with the microinjection instrument 18 and used for applying negative pressure to the needle head of the fixing needle 15 to adsorb embryos, central axes of the first through hole 24 and the second through hole 25 are perpendicular, the fixing needle 15 is made of organic glass, a suction inlet of the microinjection instrument 18 is communicated with a suction outlet of the second through hole 25, and the microinjection instrument 18 applies negative pressure to the first through hole 24 (suction inlet) on the needle head of the fixing needle 15 through sucking suction media (air or mineral oil), so that the needle head of the fixing needle 15 adsorbs the embryos, migration of the embryos is prevented, and the embryos are kept stable when being squeezed.
In accordance with the procedure of example 1, but the embryo is applied with different chemicals or the freezing process and the procedure of example 1 is repeated, before the embryo is applied with chemicals or the freezing process, the measuring procedure of example 1 is performed, the inverted microscope 1, the first operating arm 9 and the second operating arm 16 are assembled, when in use, the pressure sensor 10 is fixedly connected with the first operating arm 9 through a bolt assembly, the pressing pin 11 is fixedly connected with the pressure sensor 10 through a connecting piece, one end of the fixing pin 15 close to the second through hole 25 is fixedly connected with the second operating arm 16 through a bolt assembly, the inverted microscope 1 is opened, the live embryo and the culture solution thereof being cultured are placed in the micromanipulator 14, then the micromanipulator 14 is placed on the stage 6, the position of the stage 6 is adjusted, the first operating arm 9 and the second operating arm 16 are adjusted to the working position, the position of the second operating arm 16 is controlled by operating the second rocker 17, in the field of view of the inverted microscope 1, the fixing needle 15 is moved to the side of the embryo, so that the opening of the first through hole 24 is close to the embryo, the microinjection instrument 18 is operated, so that the embryo is sucked on the fixing needle 15 by the negative pressure in the first through hole 24, then the second rocker 17 is operated, the embryo is moved to the front side of the baffle 20, the edge of the embryo just contacts the baffle 20, the moving position of the first operating arm 9 is controlled by operating the first rocker 12, the rear side surface of the extrusion head 23 is aligned with the live embryo surface transparent belt and extrudes the embryo, the extrusion degree is set to the maximum degree which can not damage the embryo, at the moment, the lower surface of the extrusion head 23 is parallel to the bottom of the accommodating groove 21 and is slightly suspended, meanwhile, the other side of the live embryo is contacted with the front surface of the baffle 20, at the moment, the needle head of the, the embryo is not allowed to move, the acting force of the extrusion head 23 on the zona pellucida on the surface of the live embryo is transmitted to the pressure sensor 10 through the connecting rod 22, when the extrusion head 23 releases the embryo at the same set distance step by step, the pressure sensor transmits the mechanical signal data detected when the compression distance changes the interval to the computer and stores the data as a group of data describing the relation between the compression distance and the pressure, meanwhile, the video of the whole compression process is also recorded and stored in the computer, when the extrusion head 23 is found to be separated from the cell through the inverted microscope 1 or the computer displays that the stress is 0, the operation is finished, the acquisition is terminated, and the operation is carried out in the computer to obtain a first tension coefficient.
Adjusting the first and second operating arms 9 and 16 to a non-working position, releasing the embryo held on the fixed needle 15 by the microinjector 18, performing the measuring step of example 1 again after the embryo is subjected to chemical treatment or freezing treatment, chemically treating or freezing the embryo in the micromanipulation dish 14, re-placing the embryo in the micromanipulation dish 14, adjusting the first and second operating arms 9 and 16 to a working position, controlling the position of the second operating arm 16 by operating the second rocker 17, moving the fixed needle 15 to the side of the treated embryo in the field of view of the inverted microscope 1 so that the opening of the first through hole 24 is close to the embryo, operating the microinjector 18 so that the embryo is held on the fixed needle 15 by the negative pressure in the first through hole 24, then operating the second rocker 17 to move the embryo to the front surface of the baffle plate 20 so that the edge of the embryo just contacts the baffle plate 20, the moving position of the first operating arm 9 is controlled by operating the first rocker 12, so that the rear side surface of the extrusion head 23 is aligned with the transparent belt on the surface of the live embryo and extrudes the embryo, the extrusion degree is set to the maximum degree which can not damage the embryo, at the moment, the lower surface of the extrusion head 23 is parallel to the bottom of the accommodating groove 21 and is slightly suspended, meanwhile, the other side of the live embryo is contacted with the front surface of the baffle 20, at the moment, the needle head of the fixing needle 15 is positioned on the left side surface of the embryo and is used for assisting the extrusion head 23 to prevent the embryo from moving, the acting force of the extrusion head 23 on the transparent belt on the surface of the live embryo is transmitted to the pressure sensor 10 through the connecting rod 22, when the extrusion head 23 releases the embryo at the same set distance step by step, the pressure sensor transmits mechanical signal data detected when the compression distance changes and intervals to the computer and stores the data as a group of data describing, when the inverted microscope 1 finds that the extrusion head 23 is separated from the cell or the computer displays that the stress is 0, the operation is finished, the collection is stopped, and the operation is carried out in the computer to obtain a second tension coefficient.
The tension coefficients from the two measurements can be compared in magnitude to assess the extent to which the chemical or freezing process has an effect on the zona pellucida tension on the surface of the embryo.
Example 3
As shown in fig. 9 and 10, the simulation experiment was as follows: an embryo model made by using a flat plate extrusion balloon can be used for verifying the feasibility of instrument measurement, the actual extrusion model is equivalent to the embryo tension measurement process shown in figure 9, the whole process is that two standard planes extrude a sphere under the parallel state to measure tension, a circular ring is a culture dish, scales in the drawing are baffles 20 in the culture dish, scales for measuring and calculating the length are arranged on the baffles, a compressed cake-shaped object is the embryo model in the culture dish, when the embryo model is not compressed, the compression distance is 0, the compression distance of an embryo extrusion needle is changed every time, the compression distance is recorded in the following table and is converted into m as a unit to be expressed, the reading of a sensor (modified by an electronic balance) is recorded, the reading of the sensor is converted into kg to be expressed and then multiplied by 9.8N/kg to obtain the compression force, the diameter D of a contact surface under the following and upper scenes is recorded, dividing D by 2 to obtain the radius R of the contact surface and converting the radius R into m to express, calculating the contact area A by A = pi R2, calculating the compression pressure P under a certain compression length by dividing the compression force by A, and filling all the recorded or calculated values into corresponding positions of the following table.
Drawing the relation between the compression distance and the pressure into a scatter diagram 10 to perform linear regression, and calculating the pressure of the embryo model when the embryo is not compressed to be 1162.6 pa from a coefficient 1162.6 of a fitting exponential function;
the Laplace formula p =2 gamma/R can be used to obtain that the tension coefficient gamma of the embryo model is 14.5325N/m when the embryo model is not compressed, namely, the surface tension coefficient of the embryo model with the radius of 2.5cm is 14.5325N/m when the embryo model is not compressed.
The invention adds a pressure sensor on the existing inverted microscope micromanipulator, which is convenient for collecting and researching the tension data of the zona pellucida on the surface of the tested live embryo, the baffle plate in the micromanipulator is arranged to provide a support surface for the live embryo, the extrusion needle and the baffle plate act together to make the zona pellucida on the surface of the live embryo generate appointed staged deformation with the help of the fixed needle, during which, the mechanical data and the image data are recorded, finally, the tension coefficient of the zona pellucida on the surface of the embryo is calculated in a computer, which is convenient for the embryologist to judge the activity of the live embryo together with the indexes commonly used for judging the embryo, and provides reference data for the research of the live embryo, the device can also assist the research of using chemical substances for the embryo, apply different chemical substances to the embryo and then use the device for measurement, and judge the influence of the different chemical substances on, changes in the tension coefficient of the zona pellucida on the surface of embryos after recovery from low temperature freezing compared to before freezing can also be explored.
Claims (5)
1. The utility model provides a live embryo surface zona pellucida tension detection device, includes the inverted microscope, its characterized in that: the device comprises a first operating arm, a second operating arm, a pressure sensor, a pressing needle, a fixing needle and a micromanipulation dish, wherein the first operating arm is fixedly connected with the first operating arm of the inverted microscope, the pressing needle is fixedly connected with the other end of the pressure sensor and is used for pressing embryos, the fixing needle is fixedly connected with the second operating arm of the inverted microscope and is used for assisting in fixing the embryos, the micromanipulation dish is positioned on an object stage of the inverted microscope, the pressure sensor is electrically connected with a computer through a data line, the pressure sensor transmits detected data to the computer for storage, and a camera on the inverted microscope.
2. The apparatus for detecting the tension of the zona pellucida on the surface of a living embryo according to claim 1, wherein: the extrusion needle includes the connecting rod with pressure sensor's other end fixed connection, with the other end fixed connection's of connecting rod extrusion head, and the extrusion head is for being in the flat board of horizontal direction, and the contained angle between extrusion head and the connecting rod is 20~ 35.
3. The apparatus for detecting the tension of the zona pellucida on the surface of a living embryo according to claim 2, wherein: the length of connecting rod is 55~60mm, the diameter of connecting rod is 1~2mm, the length of extrusion head in left right direction is 1~2mm, and the distance of extrusion head in front and back direction is 15~20um, and the thickness of extrusion head in vertical direction is 25~30 um.
4. The apparatus for detecting the tension of the zona pellucida on the surface of a living embryo according to claim 1, wherein: the micromanipulation ware includes ascending holding tank of opening, be located the central point of holding tank and be in the separation blade of vertical direction, and the left surface and the right flank of separation blade are the plane.
5. The apparatus for detecting the tension of the zona pellucida on the surface of a living embryo according to claim 1, wherein: the fixing needle is characterized in that a first through hole is formed in the needle head portion of the fixing needle and arranged along the length direction of the fixing needle, a second through hole is formed in the side face of the fixing needle and communicated with the first through hole, and the second through hole is connected with a microinjection instrument and used for applying negative pressure to the needle head of the fixing needle to adsorb embryos.
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| CN113916721A (en) * | 2021-10-26 | 2022-01-11 | 四川省水利科学研究院 | Portable hydraulic engineering quality detector |
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