CN111690765A - Molecular marker of cotton fiber elongation main effect QTL qFE-chr.D04 interval and application - Google Patents

Abstract

The invention discloses a molecular marker of a cotton fiber elongation main effect QTL qFE-chr.D04 interval and application thereof. The molecular marker is one or more of nucleotides shown in SEQ ID NO. 1-SEQ ID NO.66, and polymorphism occurs in fiber elongation. The SNP molecular marker related to the elongation of upland cotton fibers provided by the invention can be used for early prediction and screening of the elongation properties of cotton fibers and can also be used for auxiliary breeding of the elongation molecular marker of the cotton fibers. The method can be applied to all tissues and all development stages of cotton by directly detecting DNA, and is not limited by seasons and environment, and has no problems of expression and the like.

Description

Molecular marker of cotton fiber elongation main effect QTL qFE-chr.D04 interval and application

Technical Field

The invention belongs to the technical field of molecular biology, and relates to a molecular marker of a cotton fiber elongation main effect QTL qFE-chr.D04 interval and application thereof.

Background

The cotton fiber is an excellent natural fiber, is also a leading raw material in the textile industry, and plays an important role in the world and national economy in China. Fiber quality is a complex quantitative trait controlled by multiple genes, and the composition factors comprise: fiber length, strength, fineness, elongation, regularity, maturity, and the like. The phenotypic value of the quality of cotton fiber is controlled by the genetic factors of the variety, and is also comprehensively influenced by various environmental factors and the interaction between the variety and the environment. The elongation of cotton fibers in upland fields is also one of the important indicators in fiber quality, and the elongation at break of cotton fibers is the percentage of the ratio of the elongation of the fiber when stretched to break to the original length. The higher the elongation of the cotton fiber, the better the deformability of the cotton fiber and the lower the rigidity. Phenotypic variation in cotton fiber elongation results primarily from variety effects, environmental effects, and interaction effects between variety and environment. Various studies have demonstrated that there is a significant correlation between fiber elongation and fiber length and strength, and therefore extensive studies on fiber elongation have important referential significance in understanding the mechanism by which cotton fiber length and strength develop.

Genome-wide association analysis (GWAS) is a new technical means for carrying out Genome-wide association analysis on phenotypes such as fiber elongation and the like by using a plurality of Single Nucleotide Polymorphisms (SNP) in a Genome as genetic markers and further discovering genetic variation affecting complex traits.

An SNP (single nucleotide polymorphism) refers to a DNA sequence polymorphism caused by a variation of a single nucleotide at the genomic level. As an important genetic tool, SNP has the advantages of wide distribution, large quantity, easiness in batch detection and the like, and is also a key object of functional genomics research. SNP is suitable for genetic analysis of complex traits, and research on aspects such as gene identification causing population difference, and has very important functions on construction of high-density genetic maps of plants, cloning and function research of new genes, and the like. The SNP molecular markers related to the elongation of the upland cotton fibers can be detected in each tissue and development stage of the upland cotton, are not limited by environment and season, are not influenced by whether expression exists or not, do not need to analyze the length of fragments, only need +/-analysis, are suitable for quick, large-scale and automatic screening, and can be used for early prediction and screening of the elongation of the upland cotton fibers. Therefore, the invention provides the SNP molecular marker related to the elongation of upland cotton fibers and the application thereof.

Disclosure of Invention

The invention aims to provide a molecular marker linked with a major QTL (quantitative trait locus) of the elongation rate of upland cotton fibers, so as to solve the problem that the conventional breeding has slow improvement on the elongation rate of the fibers.

The method aims to overcome the defects of poor phenotypic selection accuracy, low efficiency, long period, high cost and the like in the traditional breeding and solve the problem of slow breeding progress of quality traits such as fiber elongation and the like. According to the method, 1245 natural population of upland cotton varieties are subjected to multi-year and multi-repeat phenotypic data and genotype data to carry out GWAS data correlation analysis, and the major QTLqFE-chr.D04 of the fiber elongation is found to be positioned in the D04-52385943-D04-52505321 interval of chromosome 4 of a D genome.

The invention also aims to provide application of the major QTLqFE-chr.D04 of the cotton fiber elongation, and the QTL qFE-chr.D04 is positioned in a gossypium hirsutum genome, so that the QTL qFE-chr.D04 can be used for related research on cotton fiber elongation traits.

The invention is realized by the following technical scheme:

a molecular marker linked with a major QTL of the elongation of upland cotton fibers is disclosed, wherein the nucleotide sequence of the molecular marker is shown in any one of SEQ ID NO. 1-SEQ ID NO. 66. The SNP site is located at the 51 st base with the nucleotide sequence as shown in any one of SEQ ID NO. 1-SEQ ID NO. 66.

The mutant alleles of the molecular markers are shown in Table 1:

TABLE 1 SNP markers associated with elongation of upland cotton fibers

In another aspect of the invention, a SNP molecular marker combination related to the elongation of upland cotton fibers is provided, and the molecular marker combination is any two or more of the nucleotide sequences shown in SEQ ID NO. 1-SEQ ID NO. 66.

In another aspect of the invention, a gene chip and a kit are provided, wherein the gene chip and the kit comprise one or more of nucleotide sequences shown in SEQ ID NO. 1-SEQ ID NO. 66.

In another aspect of the present invention, there is provided the use of the above molecular marker or combination of molecular markers or gene chip or kit in cotton fiber elongation marker assisted breeding; or in the early identification of the elongation of the cotton germplasm resource fiber; or the application in cotton germplasm resource improvement.

The invention has the beneficial effects that:

the SNP molecular marker related to the elongation of the upland cotton fibers provided by the invention can be used for early prediction and screening of the elongation properties of the upland cotton fibers and can also be used for auxiliary selection breeding of the elongation molecular marker of the cotton fibers. It is expressed directly in the form of DNA, can be detected in various tissues and development stages of cotton, is free from the problems of season, environmental limitation, absence of expression and the like, and is neutral (the marker is expressed without environmental influence, and becomes a neutral marker without influence of development period, expression and the like).

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to specific embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.

Example 1

1. 1245 parts of cotton core germplasm resource field tests are respectively carried out in 8 test points of Anyang in Henan, Shizhu, Jiangsu Dafeng, Liuyang in Hunan, Xinjiang Kou, Aral, Shihezi cotton institute and Shihezi agricultural reclamation academy of sciences in 2016 and 2017 in two consecutive years. The test is set for 2 times of repetition and normal field cultivation management. Sampling and extracting DNA of each individual plant respectively in the vegetative growth period of cotton, and sending the DNA to Nohe kenyu company for genome re-sequencing. In the full-bloom stage of cotton, 1-2 cotton bolls which are normally bolled on the first and second fruit nodes around the middle part of each normal cotton plant are harvested, the total number is 30, after cotton ginning, lint samples are subjected to fiber quality detection by using HVI1000, and data collection refers to cotton germplasm resource description specifications.

2. GWAS analysis: in a related group of 1245 samples, 1122352 SNPs (MAF ≥ 0.05; deletion rate ≤ 0.2, depth ≥ 3) were used in GWAS for different traits. Correlation analysis was performed by the genome-wide efficient mixed model correlation (GEMMA) software package. For the mixed linear model analysis, the following equation was used:

y＝Xα+Sβ+Kμ+e

wherein y represents a phenotype; α and β are fixed effects representing the labeling effect and the non-labeling effect, respectively; μ denotes an unknown random effect. X, S and K are the incidence matrices for α, β and μ, respectively, and e is the vector of the random residual effect. The first three PCs were used to build the population structure corrected S matrix. The simple matching coefficient matrix is used to construct the K matrix. Analysis was performed by GEMMA software package.

3. And (3) estimating a fertility value: BLUP calculates the birth value using the lme4 software package in R (version 3.2.2). The formula is as follows:

Y＝μ+Line+Loc+(Line×Loc)+(Rep×Loc)+

where Y, μ, Line and Loc represent phenotype, intercept, mutational and environmental effects, respectively. Rep denotes different repetitions, indicating random effects. Line × Loc is used to show the interaction between the cultivar and the environment, and Rep × Loc is used to show the interaction between the replicate and the environment.

See table 1, where "observed value" refers to the number of resources with this SNP site in 1245 core germplasm resources.

4. Results

1245 parts of cotton material are planted in 12 natural environments in 6 places for 2 years, and the fiber elongation of the varieties is detected and analyzed. The 1245 cotton varieties are subjected to genome re-sequencing through an IlluminaHiseq sequencing platform, the obtained high-quality cleardata volume is 41.85Tb, and the average sequencing depth of each sample is more than 10 times. Acquisition by GWAS stable appearance of SNP molecules associated with elongation of upland cotton fibers in at least three and more environments was obtained with 66 markers on chr.d04 (table 1).

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Sequence listing

<110> Cotton research institute of Chinese academy of agricultural sciences

Molecular marker of <120> cotton fiber elongation main effect QTL qFE-chr.D04 interval and application

<160>66

<170>SIPOSequenceListing 1.0

<210>1

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>1

attctcgatg attgcttctc ctatgactaa gttactgcaa aagaatgtca cgtttgaatg 60

gaccgataaa tgtcaacaga gttttgagaa gttggaggca t 101

<210>2

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>2

gacctcacta ggtcgaccat cacatataaa gacctcattt tgtcaacctc aaagataact 60

taatcaaagt attgtatgac tatggtcaga taggccatac a 101

<210>3

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>3

aatggaagat ttcctttttg ttgggatggc ttttgaagaa tatgttatgg caaatcatct 60

ttgcttcacc tggaagattc attcaatctt gaaggtcatg a 101

<210>4

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>4

gatgatgacg gaggaatcat cgctacaata taagtcagaa ttgaacgaat gagccatctc 60

ctcaaccgca cccaatccca atctcctgca tcggccacaa c 101

<210>5

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>5

gctatccaca tcacctccag caagaatggt ctcaaccaac aagccattat tacactctaa 60

ctccaacttt tggaaccatt tatcccatgc caagcaaagc c 101

<210>6

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>6

tagatgctaa ttgcttcact tttcttgaat tagcctcacc cgtctcctag tgttggctca 60

atggaccctt ctcaaaattt tcccccacct atgtctggtc a 101

<210>7

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>7

aagaagctaa gaagcgtaaa cgtcttgctg atgacttctt tgatttattg tattctatga 60

aggtaaggca ggctttcctt aaaatttgat ctaatagttt a 101

<210>8

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>8

gaaggcgttt attaagacac cttctaagat aaattcaaat ttgaatatct aaagagtggt 60

gaagggggat atttataggc ggataatggg gggacaaata c 101

<210>9

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>9

gaagacttga aggctgccat tttggaggat attagctctc cacctatatc ggatgttaac 60

ttgaaggtat atacatgttt ttgatagttc tcatttgttt a 101

<210>10

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>10

ttaatcgtga atggaaggtg cgcattcgtc atgttcttag atcccaaaac gcggttgttg 60

atcacatagc aatgcttgcg gtacctggtc ctctaagtct a 101

<210>11

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>11

cgcactagag attgtatagt ctctttttac tagatttaat ttttttctta ttcttgtgat 60

aatcattgat gttgtacagg agtgcttgga gttgacgtca t 101

<210>12

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>12

attttccttc ctttttggct cataacattt tcttttcaac tttctctcct acattttctt 60

aggaaaaaag aaacaaaaaa actttctatt caaagaaaag a 101

<210>13

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>13

aaagaacaca aaggaggcgc gcggctataa aagtattctg cagttccttc atccttttaa 60

actatttttc agtgtagtca ctttcttcga ttcacctcac t 101

<210>14

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>14

tcctttttgg ctcataacat tttcttttca actttctctc ctacattttc ttaggaaaaa 60

agaaacaaaa aaactttcta ttcaaagaaa agaaagaaaa g 101

<210>15

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>15

tactgtgaga catatgctgg tggggttttc atgaaagagc ctttactagt gcctatgaaa 60

ttgttcttgt gactgtcttg gtaatcatgg ttactttcat t 101

<210>16

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>16

cttcattaag gtacctttaa gctagtttca aggtttgcat ttacggttta tattgggctc 60

tgtatgaggt actactattg cactgtaggc aaatagccag t 101

<210>17

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>17

atcctttgca agtgcattat cgtttgctta tttgataaat gtcctctttg tgtgtgttgg 60

aaaatagatc ttttaggctt ttaccaattt tgggactatg t 101

<210>18

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>18

tattcgtagg attatgtttt tgttggttgt attataattg ctattttcaa cgaattgttt 60

tgtattcatt tagattctat gttagcgagc cgaaattgat a 101

<210>19

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>19

tatatttata tccttatcct tcattacaat tcactattta ggtttattgc cgattgattt 60

aataggccaa aggttatgcc atcctttgca agtgcattat c 101

<210>20

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>20

ttctggtttg ggtcttatac caaggtttgg tatagcatgg gtattacatg acaagtggtc 60

tttgctaatg gggttgttac actatcatat ttcagcaaag c 101

<210>21

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>21

ttggggatgg attggttgat attgcatgat gccaaggtaa attgtagaca taaaatcatt 60

gaattgaagt gtgaaaatgg tgaaattctt cgggttgagg c 101

<210>22

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>22

atggttgttt gttttgtact gatttaggct aaccagattg ctgggatggc caataaccct 60

caggtctact ggtagcatgt gattgaaata atgtttttct g 101

<210>23

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>23

accaaagtgg ttttccaggg catgctgttg gaatttaggg ctgctctaaa ttttttttcc 60

ttcttcacga acatttatgt tgcttatact tgcataccat a 101

<210>24

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>24

actatgttta aaaatttgtc atgatttctt tttcttgaat ttgtttattg cccaggaacg 60

ggtgaaggca caggagcagc gtaagcagaa tgttatggag t 101

<210>25

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>25

tacaggttat taaattatta gtaactttat aaaatattat taaattattt gagaattttt 60

atttaagtca ttaaattatt caaatttttt tatttaagtc a 101

<210>26

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>26

aattatatat attttctcac tttagtatct aaacattttt ttaccacttt agtacttaag 60

ttcaactatc atttcgtctc aattactaaa aaaaataaaa g 101

<210>27

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>27

cctgacggga aaaaccgaca aatttaaaaa cacaaacaaa gattaaccag atccagagca 60

agaaaaaccg acaccttaaa gattcaaaca acaattaacc a 101

<210>28

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>28

acgttcaaag ctgtttaatg agaaaaaaaa cttgaactat agaagagaat ggaaggagag 60

ctttcagttg gtgcaggcgg tgcgaacaga aaaagccata t 101

<210>29

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>29

aattagattt agctaaagta attccacccg taagagaaag cttatttgca tcccatccat 60

tgagcttcct tcgaacttta tccactacaa actcaaagtt c 101

<210>30

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>30

tctgttactg caacccaaga aagacaaaaa tgggacaatg ctgattacat atgcatgggc 60

cacatattga atggtttatc tgatggtttg ctcgacacct a 101

<210>31

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>31

ccttctaatg tgtcagtgaa ctgcactagt taccaatatg aacaatttgc aaaagatgtg 60

attttcccac ttagacttcc atcttccaaa ttgtgttgcc a 101

<210>32

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>32

acctcttata cgaagcagta acggaagggt tcctcgataa ctgcccctta acccattgtc 60

ctatacccga cccgactcgt ttcgagtccc cgatttcgac t 101

<210>33

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>33

tctctctata tatataattt ggtacttgaa taatgtattt attgttagtt tggacattaa 60

aactttaata atcatataaa taagtgattg aatgaaatcg a 101

<210>34

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>34

tggaattctc aaatggacat ttcaaacctt ggaaatccaa taaaccccca ccctcacccc 60

cctaaaaaaa agaggaggag gaggctatcc atcttcaaac t 101

<210>35

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>35

gagttttttg ataaattgga tgacaatatg gttgtccgaa tatggtccaa taacacataa 60

taagagaagg atgataattt aatggaggac tatggctccg t 101

<210>36

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>36

tatgaagaaa ttgagactca atacgttagg gtacaatttc tcaaaatccc tgaattttga 60

atatcacttt tgctttataa gtaaattttc atttcgagga a 101

<210>37

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>37

gttttgttaa tgtgaacttg gttggctagt tctgctagat tgtccctctt cgagatttga 60

gtcttattga cgctttgctc gagctttaag tctcaaagcc a 101

<210>38

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>38

agtttttctc gagggttttc ccaagagatt tatctgctgt tttggaggag gttatcttgt 60

gatctcaatc tgacatttca tcctataaat aggcgtcact t 101

<210>39

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>39

tagtaaaatt tatattaaaa tcttttaaga atttagaatg ttagaagcat gtagaatttt 60

tcgaaaaatt gttcaacatg tttgagtaaa tatttacctg a 101

<210>40

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>40

ctttgctccg tttatttgca attgcgccag tttcagttag agatttgttg aattgaagat 60

cgtaatgggt ctaaatcctc taatttatga attggttgtt g 101

<210>41

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>41

gaagtataat ttggagggta attacgctaa ttaactactt agtaagaaaa ctgaccaaat 60

tgtaaaacaa gtaaacttac aaaattaaca aattaataat a 101

<210>42

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>42

gaacagtcta aactaggact ttctctttgg attttgttgt caaaaaaatt agatgtcgtg 60

ttgatgacaa aacctaggtc aacaagcaat taatgatcac c 101

<210>43

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>43

ccagtaagac aactcattag aataaattga aaataataca tgcctaaagc atggtagatc 60

cattgctttc aaatatgaaa atgctcgtaa aaaataataa a 101

<210>44

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>44

tatggctccg ttgcccaaga attcaagatg acaaagatta tttgagaacc attgaacctt 60

cttaaagaga agccaatgag catcacgagg atgagcgaac a 101

<210>45

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>45

attaaatcct taatcaaaaa tagtaaacat tttatgcttt ttttttcttc aaaattatgt 60

tttctgtatt ttagtcagta tatttgttaa aaaaattagt t 101

<210>46

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>46

tagctgtcga ctttaaaaat aaagactgaa gtcgtcaccg atccgtgatt caggtgtgat 60

cggctcacct taaaaacaaa tttgatctac gaaatttgag a 101

<210>47

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>47

agtgttacaa tggaacatct gagtatttca aggattgaac ccaagagagt cagcaattta 60

ataattttta tttaacaagc atgcaagaaa ggagtctagc t 101

<210>48

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>48

cttttactcg aattcatttg attgctcagc aagcatcgag gcttgttgca taagacactt 60

gccgagtctg ttatttctgt tacaactcca acaataactc a 101

<210>49

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>49

aaatgggtca aagtcacgtc gtttcgaaag aggttcgggt tttgttaatg tgaacttggt 60

tggctagttc tgctagattg tccctcttcg agatttgagt c 101

<210>50

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>50

atttaccctt ttatttattt taaattccaa tttaatcctt ttgaatgaca ccgttttaga 60

ggagaaggga aaattttcct tctagcccct ctatgtaact c 101

<210>51

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>51

aattcctaac aattggggag gtgaaggaag aggaagagaa caatatatct ctattccata 60

ttgatcgaat ggtagagact gcaaacaaga gtcatttttc t 101

<210>52

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>52

tcaatttcta aatagagagc tggtcataag aaaatgagtt ttttgataaa ttggatgaca 60

atatggttgt ccgaatatgg tccaataaca cataataaga g 101

<210>53

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>53

tttgtaatct tagagattta atttgtagat accctttaat ctcagccgtt gatctgtacc 60

gttggatttg gggaggctca actataaata gaggcatctc c 101

<210>54

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>54

acatcctttt gcattcattt tcttatcact taaatattgt ttgcattata catttcatga 60

gttgaacaat tttacccctt taagtgggag taagaagcta t 101

<210>55

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>55

gaattttctc aacagatcgg aattggtatt ttagtggttg atggcctctc gaaaaatatt 60

ttcaagaact atgagtgttg ttgaggtttg gaggactttt t 101

<210>56

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>56

tttgtccctt tttcttgtaa tggaacttgg atttcttttg tccaagttag ctattatttc 60

cttaaaaaaa ttgaaccaat aaaaatttta gactaaatgt g 101

<210>57

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>57

cttggataag tggatgttct tttaaagaaa aggaaatatt ccgagtttgg gattctagag 60

gaatcgtgcc ctaacgtatt gggtgtgatt tcttaaatct t 101

<210>58

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>58

taaaattata gatgaagttt gatgtgtaat tgtataaatg aaatttaatt atggtttaaa 60

cgtatactta aaactttgat tttaacttaa tcataaacat t 101

<210>59

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>59

ttatgctcct cttattttaa atgatttgag gcataacatg aagtttggtt ttccacataa 60

ttcatgcaaa tttgtgtttt tagtggcagg tgaggaagct a 101

<210>60

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>60

agatagtgat atattcaatg ccattaaaaa acatcatgta aaaagagatc gcattttaaa 60

ttctcttcga attctcaatt ttcgacgcta agacatcaag t 101

<210>61

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>61

aagatggcca gcgtatgtca cagcctcagc ccaaaattgc ttgcccaacc cagcattgga 60

caacatacat cgaaatttct ccagcaatgt tcgattcata c 101

<210>62

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>62

ttaacaccaa ctcatcttac cgtttcaccg ttaatggcaa ttaacactca gtgactaaaa 60

tgttacaaca cgataacgtg agtgactaaa atataatatt t 101

<210>63

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>63

cattctaaag aagtaattcg ttaagcaact aacagatgat ccaaagagtt ctatggtaac 60

ttttcttcgt atttcgtttc gaaaaaaggg tatatcctgc c 101

<210>64

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>64

cttaataatc cacaacaaat gttggatgcc ggtttaaata gtgaattctc tatacatcag 60

tatcacgtca taccatatcc cttatagcac gaaaataacc c 101

<210>65

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>65

aatcttttta aaactatatt taactcgtaa gtattaaata ataaaattta tgagcttact 60

cgttggattt ggtggtctcg aaccactatt tctgacacca c 101

<210>66

<211>101

<212>DNA

<213> Artificial Sequence (Artificial Sequence)

<400>66

ataaagacta aaaatgatca aattaaaagt gggtgttttt gttggtataa ttttttttat 60

ttaaaatagg tcaactttta taaaaaggtc cctatactat t 101

Claims (7)

1. A molecular marker linked with a major QTL of the elongation of upland cotton fibers is characterized in that the nucleotide sequence of the molecular marker is shown in any one of SEQ ID NO. 1-SEQ ID NO. 66.

2. An SNP molecular marker combination related to elongation of upland cotton fibers is characterized in that the molecular marker combination is any two or more of nucleotide sequences shown in SEQ ID NO. 1-SEQ ID NO. 66.

3. A gene chip is characterized in that the gene chip comprises one or more of nucleotide sequences shown in SEQ ID NO. 1-SEQ ID NO. 66.

4. A kit, which is characterized by comprising one or more of nucleotide sequences shown in SEQ ID NO. 1-SEQ ID NO. 66.

5. Use of the molecular marker of claim 1 or the combination of molecular markers of claim 2 or the gene chip of claim 3 or the kit of claim 4 for cotton fiber elongation marker-assisted breeding.

6. Use of the molecular marker of claim 1 or the molecular marker combination of claim 2 or the gene chip of claim 3 or the kit of claim 4 for early identification of fiber elongation in cotton germplasm resources.

7. The use of the molecular marker of claim 1, the combination of molecular markers of claim 2, the gene chip of claim 3, or the kit of claim 4 for improving cotton germplasm resources.