CN107338299A - The SNP marker related to upland cotton fiber elongation and its application - Google Patents

Abstract

The present invention relates to molecular labeling, specifically discloses the SNP marker related to upland cotton fiber elongation and its application.The molecular labeling is as shown in one of SEQ ID NO.1~SEQ ID NO.297；Wherein, undergone mutation at 50bp, cause elongate fiber rate polymorphism occur.The SNP marker related to upland cotton fiber elongation provided by the invention can be used for early prediction and the screening of cotton fiber extension rate character, can be also used for cotton fiber elongation molecular marker assisted selection breeding.It is showed directly in the form of DNA, and each tissue, each stage of development in cotton are detectable, not by season, environmental restrictions, in the absence of whether expressing the problems such as.

Description

The SNP marker related to upland cotton fiber elongation and its application

Technical field

The present invention relates to molecular labeling, specifically, is related to the SNP marker related to upland cotton fiber elongation.

Background technology

Cotton is the important textile raw material in China, is the valuable cargo to involve the interests of the state and the people, is accounted in the national economic development There is highly important status.With the improvement of people's living standards, the demand of grade textiles is also growing, high quality cotton is to protecting Barrier China's cotton industry development tool is of great significance.Therefore, improvement cotton fibre quality is also in current cotton breeding work A focus.The elongation at break of cotton fiber refers to the percentage extended with former long ratio when fiber is stretched to fracture. The elongation of cotton fiber is bigger, represents that the deformability of cotton fiber is better, rigidity is poorer.

Full-length genome correlation analysis (Genome-wideassociationstudy, GWAS) be apply genome in count with The SNP (singlenucleotideploymorphism, SNP) of million meters is molecular genetic marker, is carried out complete Check analysis or correlation analysis in genomic level, by it was found that the one kind for influenceing the genetic mutation of complex character is new Strategy.

SNP (SNP) is primarily referred to as in genomic level as caused by the variation of single nucleotide acid DNA sequence polymorphism, including the conversion of single base, transversion etc..SNP has distribution as a kind of important genetic tool Extensively, it is large number of, be easy to the advantages that batch detection, while be also the key object of functional genomics research.SNP is suitable to multiple The genetic analysis of polygamy shape, cause the research of the gene identification of population difference etc., for plant dense genetic map Structure, the clone etc. of new gene have a very important role.The related SNP marker of cotton fiber extension rate, in cotton Each tissue and the stage of development it is detectable, not by environment and season limit, influenceed in the absence of whether expressing, without analytic plate The length of section, +/- analysis is only needed, suitable for quick, scale, automation examination, can be used for the morning of cotton fiber extension rate Phase predicts and screening.

The content of the invention

In order to solve problems of the prior art, it is an object of the invention to provide related to upland cotton fiber elongation SNP marker and its application.

In order to realize the object of the invention, technical scheme is as follows：

Present invention firstly provides the SNP marker related to upland cotton fiber elongation, the molecular labeling such as SEQ Shown in one of ID NO.1~SEQ ID NO.297；Wherein, undergone mutation at 50bp, cause elongate fiber rate to occur polymorphic Property.

The SNP marker and its allele site undergone mutation are as shown in table 1：

The SNP marker related to upland cotton fiber elongation of table 1

Further, the present invention provides the SNP marker related with upland cotton fiber elongation and combined, including SEQ ID Any two or multiple in molecular labeling shown in NO.1~SEQ ID NO.297.

Based on above-mentioned basis, present invention also offers a kind of SNP chip, the SNP chip include SEQ ID NO.1~ In molecular labeling shown in SEQ ID NO.297 any one, it is two or more.

The primer (group) or kit for detecting the SNP marker or SNP marker combination fall within this hair Bright protection domain.

Further, the invention provides the SNP marker or SNP marker combination in cotton fiber extension rate mark Remember the application in assisted Selection, and the application in the early prediction of germplasm resource for cotton elongate fiber rate or screening.

The beneficial effects of the present invention are：

The SNP marker related to upland cotton fiber elongation provided by the invention can be used for cotton fiber extension rate The early prediction of character and screening, it can be also used for cotton fiber elongation molecular marker assisted selection breeding.It is directly with DNA's Form shows, and each tissue, each stage of development in cotton are detectable, not by season, environmental restrictions, in the absence of expression Whether the problems such as, show as that neutral (mark shows as not affected by environment, and not expressed by developmental stage and not etc. is influenceed Just turn into neutral mark).

SNP marker is suitable to quick, scale examination.SNPs often only needs +/- analysis in genome screening, without With the length of analysis fragment, beneficial to the screening of development automatic technology or SNPs is detected.

Embodiment

The preferred embodiment of the present invention is described in detail below in conjunction with embodiment.It is it will be appreciated that following real Providing merely to play the purpose of explanation for example is applied, is not used to limit the scope of the present invention.The skill of this area Art personnel can carry out various modifications and replacement in the case of without departing substantially from spirit of the invention and spirit to the present invention.

Experimental method used in following embodiments is conventional method unless otherwise specified.

Material used, reagent etc., unless otherwise specified, are commercially obtained in following embodiments.

Embodiment 1

1st, 419 parts of cotton core authors field tests in continuous 2 years 2014 and 2015 respectively in Hebei deep blue Totally 6 testing sites are carried out for state, Earthquake of Anyang station in Henan, Hubei Jing Zhou, Yancheng, Jiangsu Province, Dunhuang, Gansu Province and Xinjiang alura.Plot area 6m², per cell 1 row, line-spacing 0.8m, spacing in the rows 0.3m, road 0.8m is observed, isolate road 1.2m, often repeatedly 20 plants.Experiment is set 3 times Repeat, normal field production management.In cotton boll blowing peak period, pick in the middle part of each normal cotton plant and enclose on first and second fruit section Normal blow-of-cottons cotton boll 1~2, totally 20, after cotton ginning, gined cotton sample carries out fiber quality detection, data acquisition using HVI1000 With reference to germplasm resource for cotton Description standard.

2nd, SNP detection：Cotton plant tender leaf is taken, the cotton base of 5ug high quality is extracted using Plant Genome extracts kit Because of a group DNA.The genomic DNA of said extracted is sent to Beijing Nuo Hezhi sources Bioisystech Co., Ltd, and sequence is resurveyed for genome. The cleandata data volumes that sequencing obtains high quality are 6454Gb, average each sample 15.4G, more than 6.55 times of sequencing depth. Using BaseCalling analyses and the filtering of low quality base, effective original DNA sequence data are obtained.Effective high quality sequencing Data are compared by BWA softwares and arrive cotton reference gene group, and comparison result is removed through SAMTOOLS and repeated, and it is effectively high to obtain sample Mass-sequential.Colony SNP detection is carried out using GATK softwares.The pleomorphism site in colony is detected using Bayesian model, GATK (26,740,963 SNP sites) result SNPs is filtered, to obtain the SNPs of high quality.It is soft using ANNOVAR Part annotates to SNP testing results.

3rd, upland cotton fiber elongation full-length genome correlation analysis：Upland cotton fiber elongation genome-wide screening (GWAS) Positioning, to the genotype data obtained by the upland cotton fiber elongation character result and step (2) obtained by step (1), use The mixed linear model of genome-wideefficientmixed-modelassociation (GEMMA) statistical analysis software enters Row statistical analysis, specifically refers to (http://www.xzlab.org/software.html).Statistical model is：

Y=X α+Z β+W μ+e

Y is phenotypic character, and X is the oriental matrix of fixed effect, and α is the estimation parameter of fixed effect；Z is SNP instruction Matrix, β are SNP effect；W is the simply matrix of stochastic effects, and μ is the random individual of prediction, and e is random residual, obedience e~ (0, δ_e ²).In the model, population analysis is corrected by adding affiliation matrix in μ.Analysis has found 297 altogether SNP and upland cotton fiber elongation character are significantly correlated, are shown in Table 1.

Wherein, " effect value " value of reference system is " 0 ", is defined compared with reference system, and cotton fiber is stretched after SNP site mutation Long rate score becomes greatly negative effect compared with reference system, otherwise is positive-effect." observation value " refers in 419 parts of core authors Number of resources with the SNP site.

4th, result

419 parts of cotton materials have been carried out with 2 years, the plantation in 6 places, 12 natural environments, and has tested and analyzed these The elongate fiber rate of kind.Genome is carried out to this 419 parts of cotton varieties by IlluminaHiseq microarray datasets and resurveys sequence, The cleandata data volumes for obtaining high quality are 6454Gb, and average each sample is 15.4G, and sequencing depth is up to more than 6.55 times. Analyzing accumulative 21 calculated values by GWAS, (6 testing sites amount to 12 environment within 2 years；Average amounts to 6 between the year of each testing site It is individual；6 annual breeding values in testing site amount to 2；The breeding value of all 12 environment is designated as 1, above-mentioned to add up to 21 meters Calculation value) obtain the stable SNP marker related to upland cotton fiber elongation occurred at least in one and environment above 297 (tables 1).Utilize 419 parts of cotton core authors, 2 years 6 points of elongate fiber rate overall averages amounted under 12 environment Above-mentioned SNP effect is verified as phenotypic number, as a result shows that 67.34% SNP shows to become elongate fiber rate It is different that there is significant impact.

Although above the present invention is described in detail with a general description of the specific embodiments, On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.

Sequence table

<110>The Chinese Academy of Agriculture Science and Technologys Cotton Research Institute of Agricultural University Of Hebei

<120>The SNP marker related to upland cotton fiber elongation and its application

<130> KHP171110585.9

<160> 297

<170> PatentIn version 3.5

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tggtggcttc aatctactcc cctgctacct cgagaagata agactcaccg ttttcgatct 60

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<212> DNA

<213>Artificial sequence

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gttgaatgtg atgctaaaat attccagtaa agtatagtca tgacgaactg aaacttggaa 60

aggtacgaat gagggttcat gaaatgtgta tatggtgaaa t 101

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<212> DNA

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actcaataaa ttatttccaa agtcgcagaa ttttcttctg attcaaatgc agatcaattc 60

ttttattgaa ctagcggaaa gaccgatcag acatatacaa t 101

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<212> DNA

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aagaccgatc agacatatac aattaagctc gaataattgc aattatgtct agaagcatca 60

ttccaataat tcgcaattta tttaatcata gagtaagtcc a 101

<210> 5

<211> 101

<212> DNA

<213>Artificial sequence

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tggcccctcc ttctttaggt aaaatacttc ttgaccgaat ccaaatttac aggatttggc 60

atgtttttac catccatctc ggcttgaatt aatgctcttc c 101

<210> 6

<211> 101

<212> DNA

<213>Artificial sequence

<400> 6

tcgagaggtg gagctaatta tatgctaacc tttattgatg atttttccaa aaaagtttgg 60

gcgtttttcc tgaagcagaa aagcgatgtg ttttccgcat t 101

<210> 7

<211> 101

<212> DNA

<213>Artificial sequence

<400> 7

gcgatgtgtt ttccgcattt aagtcttgga aaattatgat tgaaaaacag aagggaaaac 60

agataaaata cctctgcaca gacaatggct tagagttttg t 101

<210> 8

<211> 101

<212> DNA

<213>Artificial sequence

<400> 8

agctaatcgc aaggaaacaa agctaaagaa aaagcagtca gcggtggttg ggactcaaag 60

taatccaccg ccaagaaaca gaatcaacga tgaagcagag t 101

<210> 9

<211> 101

<212> DNA

<213>Artificial sequence

<400> 9

tatacaaact tactaagcat gtaatgctta ctctttttat ttttccccta ttttatagtg 60

ctcggaagct cgtgaaggtt agaagattgt tggagcatcg t 101

<210> 10

<211> 101

<212> DNA

<213>Artificial sequence

<400> 10

tccatttcga ccccccttct tttgcttctt ccttcaatct agtccaattg tttccttttt 60

cttctattta gccccaaatt gcacctctgt ataaaattca a 101

<210> 11

<211> 101

<212> DNA

<213>Artificial sequence

<400> 11

caacaattgc aatgatctat ccccatcatg atgaaatttc atagattact cgggcctgtc 60

ggccaaggct atagactcgt tgaatacatc agtgtagcgc g 101

<210> 12

<211> 101

<212> DNA

<213>Artificial sequence

<400> 12

actcacttcg ctaacaagtt atcatgagta gggcgaaata acccgcttag agacgtcaga 60

aacgagtaac cggaacatgg tgcttgggtt gtcatcatgt c 101

<210> 13

<211> 101

<212> DNA

<213>Artificial sequence

<400> 13

tcttcacctc tgtgcaggat agcggattgc ttccgggata catccgtact tatgtctttg 60

tgagattttc atcttcgtgc agccataggg aaatgtattc c 101

<210> 14

<211> 101

<212> DNA

<213>Artificial sequence

<400> 14

cttgaaagaa tttaaaacac gacccctctt tgtacgattg aataaattaa aacggatgtt 60

aaaggctcgc tcatcctgaa gtaataaaat acaatgtcca g 101

<210> 15

<211> 101

<212> DNA

<213>Artificial sequence

<400> 15

gggcttcctc cagactatga atctattatt accattgtta cttccagccc agttccctac 60

acggttcaag gagtagtgtc tatgcttctt gatgctgaag c 101

<210> 16

<211> 101

<212> DNA

<213>Artificial sequence

<400> 16

atcagcatcc gacgaagtga aggggaaggg cttaatagtt cacgccataa agctttctct 60

tcattcttct ctccattaga aattccagct gcagctacta t 101

<210> 17

<211> 101

<212> DNA

<213>Artificial sequence

<400> 17

acaacattat caactggaag aacattgtct gcaagggatg tgatcccaag gaattggtcg 60

ccaaaagcca cttacacctc tcgcctgaac ttcctctaag t 101

<210> 18

<211> 101

<212> DNA

<213>Artificial sequence

<400> 18

ctcggcagct ttttgttggt cccaagattg gagaaggtgc tcatgctaaa gtttatgaag 60

gaaagtaagt ctaaaaattc taattaattg aattcaattc a 101

<210> 19

<211> 101

<212> DNA

<213>Artificial sequence

<400> 19

gtggtcactc tgtgacaatg aaggttttct ataggaagga tggtgatatt cggatcaagc 60

cggtttgttc cgataggttc tggaggtgta gccagaatcg t 101

<210> 20

<211> 101

<212> DNA

<213>Artificial sequence

<400> 20

aaaacccatc atcgccatga ccatcaccac cgataccact accatccata gcagacgccc 60

tgaaagatcc ataaacatcc ggggagggag ggtcgatatt a 101

<210> 21

<211> 101

<212> DNA

<213>Artificial sequence

<400> 21

ccatcattat aactgacgag agccctggaa attacggcgc taatattggt cgcccccact 60

tcgacctaag tggtgcggcc tttggccata tggctgtaca c 101

<210> 22

<211> 101

<212> DNA

<213>Artificial sequence

<400> 22

atgtgatccc aacgaattgg tcgccaaaag ccacttacac ctctcgcctt aacttcctct 60

aagttttaac ccttcaaagt aacaataatt ggtccatgac t 101

<210> 23

<211> 101

<212> DNA

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<400> 23

ccatagaaga aacagcttgc tataatcaat agtttcccag caagtttatg ttgttggttc 60

atacagagaa tatgaagcat tcaaatgcct tctgtgtaaa a 101

<210> 24

<211> 101

<212> DNA

<213>Artificial sequence

<400> 24

gattgcaaaa agtccaagat tccaaataag ggctttcact gctgtagccc ataatcagca 60

tttcaatgca caaaccatca gcttaaaggt taaaaaatag c 101

<210> 25

<211> 101

<212> DNA

<213>Artificial sequence

<400> 25

ccaggcccga aattaatgga aaaaggaagg gtttggaaac ctagcagcag cctccctact 60

tccagcaaag cctccagccg ccgcacgtct cgaggccgac t 101

<210> 26

<211> 101

<212> DNA

<213>Artificial sequence

<400> 26

aagcctccag ccgccgcacg tctcgaggcc gactcctcca cgtgcgctcc acaaacacct 60

gcaagcacga actcaaagag tccgaaagag aagaaacaac a 101

<210> 27

<211> 101

<212> DNA

<213>Artificial sequence

<400> 27

actggcatta aaacccttgg ggccaatctt ggcccatttg acattacaat ccctttccct 60

ttagctttaa tgtttgtctt cttatctttt agctgccctt c 101

<210> 28

<211> 101

<212> DNA

<213>Artificial sequence

<400> 28

ttggatgggc tcagtttaac aaagaagtcg gatagaaaaa gtaagtgata gagatgaata 60

atagaactta aacatcaaga aagattcgat actataatga g 101

<210> 29

<211> 101

<212> DNA

<213>Artificial sequence

<400> 29

aaacccatac ttgaagccca cctaatctaa cccaacgtaa cccaaaaccc agaagcccaa 60

aaccccaaga cccaaatcag aaaacaccaa acaaccctaa c 101

<210> 30

<211> 101

<212> DNA

<213>Artificial sequence

<400> 30

gtggcaactc cctttttttc ataaaaccga aatttcattt tcaaattttc aataaatcgc 60

cacaattagc gaccaagcta aagattttac gtcgctacaa t 101

<210> 31

<211> 101

<212> DNA

<213>Artificial sequence

<400> 31

aactgtatat gataaaaatg tccgagtgaa tgtattaaag aataggatac gattgacacg 60

ccaatagggt tatttgcgca ttgtacggga tgtatgtgtt g 101

<210> 32

<211> 101

<212> DNA

<213>Artificial sequence

<400> 32

actgatgtgg tggaggaatg tattgatact gattatgtga tgcctacgga cttttttcgg 60

tagaaaaaga tagcgttaag cgattatatt taaaagcaca t 101

<210> 33

<211> 101

<212> DNA

<213>Artificial sequence

<400> 33

ttaggaagat gagcaatcta agcttggatg gtcacaaata tattttgatg gagcataaat 60

ttgactaaga tttcagtttt tcaattattt agagtcttcc t 101

<210> 34

<211> 101

<212> DNA

<213>Artificial sequence

<400> 34

gattgttgaa agacttggga gttgttcaaa gtcacattaa tctatattgt gacggtcaga 60

gcgctattca tttagcgaaa aatcaagtct atcattcaag a 101

<210> 35

<211> 101

<212> DNA

<213>Artificial sequence

<400> 35

gtcctcgaaa atcttaccag taaataagtt tggatatcgt tctttcatgg cattctcggt 60

ttcccaagta gcttattcga ctctgtgttt gagccataac a 101

<210> 36

<211> 101

<212> DNA

<213>Artificial sequence

<400> 36

aaacaagtgg actaagccga actagtcctc atattttgaa tacctaggtg agttgtatgt 60

aaataataca actacattgt caatatatgt gttcgaattg a 101

<210> 37

<211> 101

<212> DNA

<213>Artificial sequence

<400> 37

ttatggaaaa accaccacta ttgtagataa actttcactt aataacattg tggacaagct 60

accacttttg tagataaatt gtcactttat tatattatgg a 101

<210> 38

<211> 101

<212> DNA

<213>Artificial sequence

<400> 38

taggatcacc cgtccggact agattctttt taccgtcaat tccttttcaa agatccatcg 60

aaatttcctt tcattcaacc gggatttctt cctcttttat c 101

<210> 39

<211> 101

<212> DNA

<213>Artificial sequence

<400> 39

agtaaatccg gatgattttt ttcaaaatcc gaacacttgg catgtgaaac tcccattggt 60

aaactatgct atcgtggaga tgcactagtc agatagggta t 101

<210> 40

<211> 101

<212> DNA

<213>Artificial sequence

<400> 40

accacaaccg aggagatgac tgaaaaccaa gaaaatccgc tacctcttga gattgctgtt 60

aatcagaatc ctgctctgca tactatgtat gattacgcta a 101

<210> 41

<211> 101

<212> DNA

<213>Artificial sequence

<400> 41

aaaaggatgt tttaatgtag gaatatgtaa tattggtgag gcacgagctg agcccttgat 60

tgccttagtg tttttggctt tgattagcct cataaggaaa g 101

<210> 42

<211> 101

<212> DNA

<213>Artificial sequence

<400> 42

aacttagcaa attttcattc attcatttca ttacctctta aggattatag accacattta 60

cattcaaaat ataaaacatt tacaagtgac aaacaccaga c 101

<210> 43

<211> 101

<212> DNA

<213>Artificial sequence

<400> 43

attggtgaat tcgatgctcc taatgcttta atttcatatt ttatacttat gagagcatag 60

gagagcggaa ggaacgagaa acgggccaaa aacggagaaa a 101

<210> 44

<211> 101

<212> DNA

<213>Artificial sequence

<400> 44

gcataaaaat tcgtaatcaa gataatacga tatgagtaca agaggccgta gacggagccg 60

aggaagtgct cgagcaagat cttcgtcttc gagacatatg c 101

<210> 45

<211> 101

<212> DNA

<213>Artificial sequence

<400> 45

cacggagaaa gagctgctag ctatgatttt tgcattcgat aaatttaggt catatctaat 60

attgtctaaa gttgtcgttt atactgacca ttctactctt c 101

<210> 46

<211> 101

<212> DNA

<213>Artificial sequence

<400> 46

accctaatca gtcacttaaa tcaacactta atcgtaactt caacgagata gacaaacctt 60

tatccatcaa aagcccttac cttcgatcga acgatggcga t 101

<210> 47

<211> 101

<212> DNA

<213>Artificial sequence

<400> 47

ctaaaaggtg tgttgcaaca tacaagcaac gtgttgcaac ataaggagtg gtccactcac 60

tcctcaattt aagttggggt cctacacaca caactagcac a 101

<210> 48

<211> 101

<212> DNA

<213>Artificial sequence

<400> 48

acattctagt gaagaatcct ccttgtattc aaaaactaga cctctcactc atggtggttg 60

attctacaca ggctctaatc gtacatgtcg gtgtgcaccg t 101

<210> 49

<211> 101

<212> DNA

<213>Artificial sequence

<400> 49

tagttttgaa aaagatcatt cccatacaga aagattttcg aggaaaatgg atgcctaatt 60

gggaggggcc ttacgtggtg aagaaagctt tttccggagg a 101

<210> 50

<211> 101

<212> DNA

<213>Artificial sequence

<400> 50

gtcttcttca tggagacaaa acttcaaaaa aaaatagatg gaacgagttc agagatgttg 60

tggattcatg aacggtattg aggttaattc tgaaggttca a 101

<210> 51

<211> 101

<212> DNA

<213>Artificial sequence

<400> 51

atactggatg aaatttgagc ttctcaggca actcaagctt gtaggccacc ttgcctacct 60

ttttcagaat tcgaaatggc ccctcatacc accgaacgag t 101

<210> 52

<211> 101

<212> DNA

<213>Artificial sequence

<400> 52

gccgaagata cgtctccagc aatgtattca ctcgttcggt ttgcccatca gtttggggat 60

gcatgctagt ggaaaagttt aagtctgaac ccattaactt g 101

<210> 53

<211> 101

<212> DNA

<213>Artificial sequence

<400> 53

ccctccaatc gtatactctg aagcatctgt gcgtacctca tacgcctttg aaaaatttgg 60

caatgcgagt acaggttcgc ttgtcatcgc ttgcttcact t 101

<210> 54

<211> 101

<212> DNA

<213>Artificial sequence

<400> 54

tcggcaatta cccgaacttt actttcatcc attcgaatcg tgccacctcc cacaatatgg 60

catagaaatg gcatttctcg ttaggcaaag gagcatttct c 101

<210> 55

<211> 101

<212> DNA

<213>Artificial sequence

<400> 55

tgccacctct cacaatatgg catagaaatg gcatttctcg ttaggcaaaa gagcatttct 60

cctttttgac atatagctta ttttctcgta gagtttggaa c 101

<210> 56

<211> 101

<212> DNA

<213>Artificial sequence

<400> 56

tctcgtagag tttggaacac ctcccccaag tgtcccacat gctcttcgag cgtcttacta 60

tacaccacaa tatcgtcaag ataaacaacc acaaaacgat c 101

<210> 57

<211> 101

<212> DNA

<213>Artificial sequence

<400> 57

ctttgggcct tcgtcattct tttccctttg atccttgttt cccctaccat tgtctttggg 60

tttcgacttg ggtttggaag aatcattatt atcaaacttg c 101

<210> 58

<211> 101

<212> DNA

<213>Artificial sequence

<400> 58

ggactcctcg agattaacca ctcgaatttc caagctcgac agcatatccc tcgatcgact 60

agcctttcta gccctcgcac gggtctccat tggctcattc t 101

<210> 59

<211> 101

<212> DNA

<213>Artificial sequence

<400> 59

aaaaaggttt atggcagtta atctcgatat gagcaaagca tacgacagga tggaaaggga 60

tttttttaag gaagtgatgt ttagaatggg attcaacaaa a 101

<210> 60

<211> 101

<212> DNA

<213>Artificial sequence

<400> 60

acaatctttt attctgtggt tattaatagc cacatcggag agaaatttag acctattaga 60

ggacttcggc aaggtgacct gttaagccca tttttgtttc t 101

<210> 61

<211> 101

<212> DNA

<213>Artificial sequence

<400> 61

ttttattctg tggttattaa tagccacatc ggagagaaat ttaaacctac tagaggactt 60

cggcaaggtg acctgttaag cccatttttg tttcttactt g 101

<210> 62

<211> 101

<212> DNA

<213>Artificial sequence

<400> 62

attagaggac ttcggcaagg tgacctgtta agcccatttt tgtttcttat ttgtggagag 60

ggtttatcaa gcctaatgag actagctttg aatgagggtt t 101

<210> 63

<211> 101

<212> DNA

<213>Artificial sequence

<400> 63

tttggggaag caataagcag gggggctaat ttattcaaag aaattttacg cgaatataga 60

tcttgttcag gccaaagggt aaattttgaa aaatcaacgg t 101

<210> 64

<211> 101

<212> DNA

<213>Artificial sequence

<400> 64

agacatagcc atatgcgcgt tcattgcacg caaagcaaat agtgattttt gacttagggc 60

atcagcaaca acattagcct ttcccggatg atagtcaatc a 101

<210> 65

<211> 101

<212> DNA

<213>Artificial sequence

<400> 65

catggctttc tggtgaggat ggtggtttca aattacaaac gttggacagg cagcaaaacg 60

gacatgtgtt taagtcacca aagatgattc ttcttgcgaa a 101

<210> 66

<211> 101

<212> DNA

<213>Artificial sequence

<400> 66

gctgttttgc caactcttca ataatgtttg cggcgttttc acaaattgca ccgctaatac 60

ctcacctatt gcggcgtttc tgctaagcgc cgccactgcc a 101

<210> 67

<211> 101

<212> DNA

<213>Artificial sequence

<400> 67

gacaactgaa tttttgtttt aaaaaaacgg tttcgacagc ttggcgactt cgctggggac 60

tttagagagt cgagccgtga attggttatg ttttgtcttt t 101

<210> 68

<211> 101

<212> DNA

<213>Artificial sequence

<400> 68

gccggtgctt caaacttcca ggctggatcg ggctctaacc ccgaaaaact ccatgctaat 60

ttttccattc ctgactttga tgaagtggct gaaaaagaaa a 101

<210> 69

<211> 101

<212> DNA

<213>Artificial sequence

<400> 69

ctgcgtgcta ggtcgtgtaa ctgcttgact tgcaaccctt tgaaacctac aggggacaca 60

ttgccatgtc ccatgcctat gtggacaaga aataggccat t 101

<210> 70

<211> 101

<212> DNA

<213>Artificial sequence

<400> 70

taactcgagc tgccctactc gttgcattta ctctggtcaa ctatcccaca tgcatcgttt 60

ccttttcatt gaagtccgat gtaccaccca cctctcccat a 101

<210> 71

<211> 101

<212> DNA

<213>Artificial sequence

<400> 71

agcactctac tggcttcttc tcacattcgg cctccttggc ttcgacacct cttgcgctcg 60

aaccaagtgc cacggcctta cctaccggct tctccttaag c 101

<210> 72

<211> 101

<212> DNA

<213>Artificial sequence

<400> 72

tcttgggttt cttctttcca actcgtggtt tcctattacc accattgtca ccgttgccat 60

tgccatccat actatcttcc ttgtgatcta tttcacatat g 101

<210> 73

<211> 101

<212> DNA

<213>Artificial sequence

<400> 73

ccgttgccat tgccatccat actatcttcc ttgtgatcta tttcacatac gccccttccc 60

tcggacttgg aagacccaag cttgtctttc ccttgaccaa t 101

<210> 74

<211> 101

<212> DNA

<213>Artificial sequence

<400> 74

ccccttccct cggacttgga agacccaagc ttgtctttcc cttgaccaag cttcaccatg 60

gattcaacta ccatcatggc ttccgacagc ttttggacac c 101

<210> 75

<211> 101

<212> DNA

<213>Artificial sequence

<400> 75

tcggggaagc aggattcgcc gtcgtagctt caatctgatc cactgcatca ctagggaagt 60

aagattcgcc attgcggctt caatcttttt aattgcaatg t 101

<210> 76

<211> 101

<212> DNA

<213>Artificial sequence

<400> 76

gtcatccttc gacagaacat agtgatatga gctttaggac aactagtccc attatacttt 60

tcaaacttca gcattttaaa cttaggcggg agcactaaat c 101

<210> 77

<211> 101

<212> DNA

<213>Artificial sequence

<400> 77

gtacggatgt atcccgaaag cgattcgcta tcctgcacgg aggtgaaaac ttcacgaagg 60

agtagcttct cactcccact taaagggtgt aaccgagcgg t 101

<210> 78

<211> 101

<212> DNA

<213>Artificial sequence

<400> 78

tacggatgta tcccgaaagc gattcgctat cctgcacgga ggtgaaaatc tcacgaagga 60

gtagcttctc actcccactt aaagggtgta accgagcggt t 101

<210> 79

<211> 101

<212> DNA

<213>Artificial sequence

<400> 79

taaagtggct gatccttggt gcctttacta ctgcccagat gcgtccccga taatggtgaa 60

gtgatattaa ccagtttgaa attttcttgg ttaaaagaaa a 101

<210> 80

<211> 101

<212> DNA

<213>Artificial sequence

<400> 80

aatccaagta actcctctgg aaacacattg gaatactcac aaaccactga cactgattca 60

aacttcgatt cagacagttt attatccaat gcataagcaa a 101

<210> 81

<211> 101

<212> DNA

<213>Artificial sequence

<400> 81

ttagactaag aacatgttta tgttcatcta ctaatacaag ttgtcttttc gtattacgat 60

tcgaccacgt aataccgctt aatatgtaac agcccgattt t 101

<210> 82

<211> 101

<212> DNA

<213>Artificial sequence

<400> 82

aactgtctaa gtgatgcatg actgggtagg taaaaacacg ttaggggtca ctagagtaag 60

gactttgcag attgtaaaag ggttgtcaca tagaacaacg a 101

<210> 83

<211> 101

<212> DNA

<213>Artificial sequence

<400> 83

tccgaatctt ggttgttttt acacaattca cttaacaaaa ttactgaaca gcctttttat 60

acttttgact taagacattt ctttaacgtt taccataatt c 101

<210> 84

<211> 101

<212> DNA

<213>Artificial sequence

<400> 84

ccgaatcttg gttgttttta cacaattcac ttaacaaaat tactgaacta cctttttata 60

cttttgactt aagacatttc tttaacgttt accataattc a 101

<210> 85

<211> 101

<212> DNA

<213>Artificial sequence

<400> 85

tatgcatagt tttccatgtt tttcgcttct ctagcgtcct ggcttgtcct tctcggtgga 60

cacacttaaa caacaacacc taaaatataa acataacatt t 101

<210> 86

<211> 101

<212> DNA

<213>Artificial sequence

<400> 86

tgcatagttt tccatgtttt tcgcttctct agcgtcctgg cttgtcccta tcggtggaca 60

cacttaaaca acaacaccta aaatataaac ataacatttt g 101

<210> 87

<211> 101

<212> DNA

<213>Artificial sequence

<400> 87

ctatctttgg tagatacctt cacattgtca atttaatgat cgcatacttg tacaccactt 60

acaattctta acgtatcatt catctaactg cttggctatc t 101

<210> 88

<211> 101

<212> DNA

<213>Artificial sequence

<400> 88

cacgaatcaa atacgatgac aatgtcccaa acatggtctt acacgtaaac acaaattgat 60

gccaacatcc cagacgtggt cttacacgaa aacacatatt a 101

<210> 89

<211> 101

<212> DNA

<213>Artificial sequence

<400> 89

caaatacgat gacaatgtcc caaacatggt cttacacgta aaaacaaatc gatgccaaca 60

tcccagacgt ggtcttacac gaaaacacat attagaaatc c 101

<210> 90

<211> 101

<212> DNA

<213>Artificial sequence

<400> 90

caaaatcatc tgaatcaatt ctgaattcat cggaacacat atttgttttt tgaacctcaa 60

acaattattg gtatcaactc aaaactctat ttcaacactc g 101

<210> 91

<211> 101

<212> DNA

<213>Artificial sequence

<400> 91

cactctcggg tatatcgact gagtctcaca aattttcact catactctgc cactgtcatc 60

cgaccctgtt tcaattcaag aaattccttg cgtttctgat c 101

<210> 92

<211> 101

<212> DNA

<213>Artificial sequence

<400> 92

tgaatggtaa gttaatttcc cgttatacga acttactaag cataatatgt ttacattgta 60

ttatttttcc cgttttatag taattcgaaa gctcgttggg t 101

<210> 93

<211> 101

<212> DNA

<213>Artificial sequence

<400> 93

gtaagttaat ttcccgttat acgaacttac taagcataat atgcttacac tgtattattt 60

ttcccgtttt atagtaattc gaaagctcgt tgggttggaa g 101

<210> 94

<211> 101

<212> DNA

<213>Artificial sequence

<400> 94

cctccaccct ctcctgtctt tcaatcgatt ctcgatatgc cacaaggtaa agaacttgtt 60

agaactgata agcctcctat agataaaatt cataaatatg g 101

<210> 95

<211> 101

<212> DNA

<213>Artificial sequence

<400> 95

tccaccctct cctgtctttc aatcgattct cgatatgcca caaggtacat aacttgttag 60

aactgataag cctcctatag ataaaattca taaatatggg g 101

<210> 96

<211> 101

<212> DNA

<213>Artificial sequence

<400> 96

taacctcggt aatgtcagtg gtagtcgagg tacgataata gattcaacta tcaaatttga 60

agcacgagca ttggcaagga catatactat tcgtgcacat g 101

<210> 97

<211> 101

<212> DNA

<213>Artificial sequence

<400> 97

tagtttctca ccttggggtg caccagtttt ttttgtcaag aaaaaagatg gatccctgag 60

actatgtatt gattaccgac aactcaataa ggttacaatc a 101

<210> 98

<211> 101

<212> DNA

<213>Artificial sequence

<400> 98

caacaagtga tcctaatcta tcacacaccc atgacaattt cctcaccttt gtaccttaaa 60

gaatgatatt ctccctaata aattcaccca tttgaattca a 101

<210> 99

<211> 101

<212> DNA

<213>Artificial sequence

<400> 99

ataaacattg ttgtcacacc ccagtttagg cgggtttaag gagaaggcgt atggtagtga 60

aagaacctat gtttggcggg ttgtaattca cccatttgtt t 101

<210> 100

<211> 101

<212> DNA

<213>Artificial sequence

<400> 100

caaccttagt gaccatactg catgtggtaa atgactagat agaattaaag gagaaatgta 60

gttaatgaga acagatgtca agcaggtgca ctctaagtgc a 101

<210> 101

<211> 101

<212> DNA

<213>Artificial sequence

<400> 101

attaccttgg cattcaccat taaaaatgac catttacata tgaccttatc tatacatgcc 60

acaatcttgt aatcatttac attcataccc tttcacaccc t 101

<210> 102

<211> 101

<212> DNA

<213>Artificial sequence

<400> 102

accatttaca tatgacctta tatatacatg ccacaatctt gtaatcattc acattcatac 60

cctttcacac cctcattcgg agagctgata ctgttgagaa a 101

<210> 103

<211> 101

<212> DNA

<213>Artificial sequence

<400> 103

accttatata tacatgccac aatcttgtaa tcatttacat tcataccctg tcacaccctc 60

attcggagag ctgatactgt tgagaaactt cgctggcata g 101

<210> 104

<211> 101

<212> DNA

<213>Artificial sequence

<400> 104

tgccacaatc ttgtaatcat ttacattcat accctttcac accctcattt ggagagctga 60

tactgttgag aaacttcgct ggcatagacc gcctaagtcg c 101

<210> 105

<211> 101

<212> DNA

<213>Artificial sequence

<400> 105

ggtgcacaag ctccgtatcc aggctggtgt aaggaggcaa cggagggata ttgcatatga 60

tgatgaaaag aagacacatg acaatatgtt ctgcctctgg t 101

<210> 106

<211> 101

<212> DNA

<213>Artificial sequence

<400> 106

taatcaagat agcaaatttg gctaagatga gatgagaaaa gacaatggtt aagatgggat 60

tttattagga gtaaagcttg aggtgaatac attttgtcaa g 101

<210> 107

<211> 101

<212> DNA

<213>Artificial sequence

<400> 107

gttcctcaga taccttcatg caatctttcc aaaatttctt ttgcctcgat ggcatccaca 60

cacctcaaca atacttgatc ttttcccttt ttatacaaaa t 101

<210> 108

<211> 101

<212> DNA

<213>Artificial sequence

<400> 108

tgaactctcg aagcaattca atgaggtctt gttttacttc ttcagctatg cacgtgccga 60

tctttaccac ctttccctct tccaggatca ctgtctctat c 101

<210> 109

<211> 101

<212> DNA

<213>Artificial sequence

<400> 109

cacgatttca tgtgggaact atccaccggt aacctattta aggtagataa gggtaaaaca 60

ttcaaggctg atccattatc aattagcact cctgctaacg t 101

<210> 110

<211> 101

<212> DNA

<213>Artificial sequence

<400> 110

tcggtcttct aattgttttg gtaggtctac tcttgttttc tctatttcta ctgcgtcatt 60

gagatccgga accacgagat taataggatt atcccctgga t 101

<210> 111

<211> 101

<212> DNA

<213>Artificial sequence

<400> 111

ctctgtgggt acacccttgg ttgcgtctgg acattagttg gggcgaaacc tagagggtag 60

gcagggtctt cgtgatcatc cccagaattt actgcggggt t 101

<210> 112

<211> 101

<212> DNA

<213>Artificial sequence

<400> 112

ctgtgggtac acccttggtt gcgtctggac attagttggg gcgaaacttg gagggtaggc 60

agggtcttcg tgatcatccc cagaatttac tgcggggttc t 101

<210> 113

<211> 101

<212> DNA

<213>Artificial sequence

<400> 113

ttttaacctt gcttcttcat tttggaattc taataaactt ttctccaaga cactctctca 60

agctcaggca tcttgaaatt tctcttccca ctgatcagct t 101

<210> 114

<211> 101

<212> DNA

<213>Artificial sequence

<400> 114

ttataagaaa actcacactc agctaaccct tgtgttgccg gtacgaactg tcttgactta 60

tattgtctta acacgagcag aggggcatat ctgacagctc c 101

<210> 115

<211> 101

<212> DNA

<213>Artificial sequence

<400> 115

caaatctgta acggcttcat ctatatgccc caatgcttta ggaaaaataa ccagcccgta 60

catactcaaa gcaaaaacat cgactttctt cttcgtatca g 101

<210> 116

<211> 101

<212> DNA

<213>Artificial sequence

<400> 116

tcatagtacc gttttattta attaccccta ttaacacgac tcgaactcgg acagatacac 60

agatgcaacc aacacaccag tttggcaccc agtgccttat c 101

<210> 117

<211> 101

<212> DNA

<213>Artificial sequence

<400> 117

gcaaccaaca caccagtttg gcacccagtg ccttatcgga taattcgaaa tagtaaattg 60

acacccaatg tctcatcggt taaaccaaag taaattggca c 101

<210> 118

<211> 101

<212> DNA

<213>Artificial sequence

<400> 118

tgctttagat aaattcagat cttacctatt gggaactaaa gtaattatcg tttctgatca 60

tgcagctttg aaatatctga tagggaagaa ggaggcaaaa c 101

<210> 119

<211> 101

<212> DNA

<213>Artificial sequence

<400> 119

agtatcatcc acgtgagcga ggtcaatttc gacaaggaag aggattcata cccccggaaa 60

acagatttca cgggaatcta acatttacga atccggtaca t 101

<210> 120

<211> 101

<212> DNA

<213>Artificial sequence

<400> 120

ctgtaccact tatttcgttt acacttttat tatcgtcttg actttgccca ttgaaccatt 60

cagaatcgct agggatactc aaaaatctcg tataactcgc a 101

<210> 121

<211> 101

<212> DNA

<213>Artificial sequence

<400> 121

tgtgataact cattattcac tttttgtgaa aactatagct aaaggtagag gcagatgtag 60

taacatccta gccagagaca gtggtaggtc tagctgatga a 101

<210> 122

<211> 101

<212> DNA

<213>Artificial sequence

<400> 122

gtcatcttca aactaaacaa ccaaccaccg ctttgtcttc ttgccattct gccgattgtt 60

gctcctaaac cgcatcttga taatgatata tcaacaataa t 101

<210> 123

<211> 101

<212> DNA

<213>Artificial sequence

<400> 123

ctatgcaaga aatttgtatg agtggcttat gttaaacttg aaaattaaac atggttggtg 60

tttgggagat gtagactaac gatgtctttt tgggatcacc a 101

<210> 124

<211> 101

<212> DNA

<213>Artificial sequence

<400> 124

tgatattata gggaatatgc tccgacatgg aaaacttccc gttactcgag ttccagccgt 60

gtagagttct tggaaaaagt gttgttgtag gatttcataa t 101

<210> 125

<211> 101

<212> DNA

<213>Artificial sequence

<400> 125

ttctaaggca gcataatccc agaaggggtt aaacgacatt tagatcatcc ttatcctgaa 60

gttaatggaa gcaagttcca ttcagcaata cgaccaatta g 101

<210> 126

<211> 101

<212> DNA

<213>Artificial sequence

<400> 126

catcgactcg tagtcgaagt aaccctgaac tcttcctatc ctatgacata ccaactatat 60

ctgactctgc ccgaacagtt aataggatat ttaattcatt t 101

<210> 127

<211> 101

<212> DNA

<213>Artificial sequence

<400> 127

actcttccta tcctatgaca tgccaactat atctgactct gcccgaacat ttaataggat 60

atttaattca tttttcaata tcattcaatc aaacatatgg a 101

<210> 128

<211> 101

<212> DNA

<213>Artificial sequence

<400> 128

ttaaagcttc ctggatgaag cgagaagggt atttggtgag cctcttttaa caatacctgc 60

ctcaaataat ttcctgttgg cacatacatc ttacccatta t 101

<210> 129

<211> 101

<212> DNA

<213>Artificial sequence

<400> 129

ctacctgcca tacaggtctg cacgccaggt agtaacactt ctttaccttt agcagttaca 60

agtcgtacgc ctctagtttg gtagtctaca atgatgttat g 101

<210> 130

<211> 101

<212> DNA

<213>Artificial sequence

<400> 130

taagttcaat tgaacttagt gagttctaaa tagacaaaaa aatgattatc agggtactga 60

taatcaacct aggaataaag acattttcga gactcaatct g 101

<210> 131

<211> 101

<212> DNA

<213>Artificial sequence

<400> 131

caaaggtgaa acatgtcggc tataggcact cttttatagc actgtcaaca gaaaataagc 60

ttagtaaaga ggttagaaat cccactatca cccttgaaat c 101

<210> 132

<211> 101

<212> DNA

<213>Artificial sequence

<400> 132

aaccttttta caagccttcc ataataccct gcctagccga ggaagctaca tacctctgta 60

aagctcttct gcagattcca tgcaggtata gtctagatct t 101

<210> 133

<211> 101

<212> DNA

<213>Artificial sequence

<400> 133

ttagggtcta tcaatgcata tatgttggtg taaaataaag aaaatttact cgtaataatg 60

ttgagggctt cagtgtccac ttttttctta catgacatat a 101

<210> 134

<211> 101

<212> DNA

<213>Artificial sequence

<400> 134

atactaagcg gtattatgtg atcggatcat aatacagaaa gatagttggt attagtagat 60

aaacctaaac atgtccttag cctaataaaa aaatgagtaa a 101

<210> 135

<211> 101

<212> DNA

<213>Artificial sequence

<400> 135

actaagcggt attatgtgat cggatcataa tacagaaaga tagttggcag tagtagataa 60

acctaaacat gtccttagcc taataaaaaa atgagtaaac c 101

<210> 136

<211> 101

<212> DNA

<213>Artificial sequence

<400> 136

tgctttgatt caattggttc aagcccacac tcgaagtaat tcatggtaca agaatagcgg 60

agaagatcat ttagttgaaa tttggaaaac attaaggatc c 101

<210> 137

<211> 101

<212> DNA

<213>Artificial sequence

<400> 137

tacgatttcg ctctaagggt ttattgctat aaatatcaca aactgagtct attttcaaaa 60

ttttaatttt ccactgtaca agaaaaccat tttcaaacca g 101

<210> 138

<211> 101

<212> DNA

<213>Artificial sequence

<400> 138

gagggggctt gggcttacaa aaggctgaag gttgaaatca aacgcaaaag atgaaataca 60

attagaagct catccacaaa agagatatgt tgggctcaga t 101

<210> 139

<211> 101

<212> DNA

<213>Artificial sequence

<400> 139

tgggcttaca aaaggctgaa ggttgaaatc aaacgcaaaa catgaaataa aattagaagc 60

tcatccacaa aagagatatg ttgggctcag atgctcttta c 101

<210> 140

<211> 101

<212> DNA

<213>Artificial sequence

<400> 140

actgcgttat tcttattagg ctaaaaataa ttttgattca tagagttaac tttatcttgc 60

tttgtatatt tggggaaggt tgcatagtcg gtttgttagc t 101

<210> 141

<211> 101

<212> DNA

<213>Artificial sequence

<400> 141

caactccatc ccttaagaaa tttttgtcct cgaaaacctt accagtaaag tggttcagat 60

attgcttcct catcgtttcc ttaggttccc aggtagtttc t 101

<210> 142

<211> 101

<212> DNA

<213>Artificial sequence

<400> 142

ttaacatctg tcgaagaaat aatatgtgaa ggatctaacc gatactgtcg caacatagac 60

acatgaaagg cattgtgaat cttttctaat tctgatagta a 101

<210> 143

<211> 101

<212> DNA

<213>Artificial sequence

<400> 143

acatgaaagg cattgtgaat cttttctaat tctgatagta aagctaatcg gtatgcaaca 60

ggtctaattc tctctgtaac ttcatataac ccaataaatc g 101

<210> 144

<211> 101

<212> DNA

<213>Artificial sequence

<400> 144

catattagaa ataaattggg actaaactag gtattcaaag aatttttcag aaaacattaa 60

aatttttcaa aggtgcaagg gacacacgcc catgaggtta g 101

<210> 145

<211> 101

<212> DNA

<213>Artificial sequence

<400> 145

ccatgtaact ctctgacttg taacttaatt aagtacaggg gtcacacggc caagtcacaa 60

gcttgtgtgc caggcagtgt gatcaattcc aaatactcta t 101

<210> 146

<211> 101

<212> DNA

<213>Artificial sequence

<400> 146

atgtcgagat caacattccg ctgttggacg ccatcaagca aataccgcgg tatgctaaat 60

ttcttaaaga gctttgcacc aacaagcgaa aattaacagg t 101

<210> 147

<211> 101

<212> DNA

<213>Artificial sequence

<400> 147

cccaatacgt tagggtacaa ttcctcataa tccccgaact ttgaatatcg cttttatttt 60

atgcgaaaat cttcatttcg agaaagtaac aggccacacc c 101

<210> 148

<211> 101

<212> DNA

<213>Artificial sequence

<400> 148

tgtaatgaaa ttttcgttat tatcagcctt agaagatgat ttttttttat aaaaaatcga 60

cattcattct tgaaatgtcc cggcttatca caataataac a 101

<210> 149

<211> 101

<212> DNA

<213>Artificial sequence

<400> 149

tattgagaag gaggaaagtc aaccaacagt tgaaattcct acaccagacg agccagaacc 60

tacaaagtct gatgaggtaa aatctaaact agtaaattcg g 101

<210> 150

<211> 101

<212> DNA

<213>Artificial sequence

<400> 150

tttgaggcta ccaaagaaac aatatggctt cgaaagttcc ttacaaatct tgaagtcgtt 60

catggtatgg gaaaagttat catattgtat tgtgacaata a 101

<210> 151

<211> 101

<212> DNA

<213>Artificial sequence

<400> 151

gccaaaatag ccaaaaattt gtccatcttc tccatggtga acgaaatcag aaagggggaa 60

gccatattta gggttttcaa gcttccaagc tctatagtaa g 101

<210> 152

<211> 101

<212> DNA

<213>Artificial sequence

<400> 152

tttagactaa ttttttgttg agaatttgaa cttgtaagga gttgctctta gacagatagt 60

gggaacctcc taatggattc tgatggctta ttttttagag c 101

<210> 153

<211> 101

<212> DNA

<213>Artificial sequence

<400> 153

gcaaacattt gatattttgg accgaggtaa gtttgtatgt aaataatgcg ttgtttttac 60

ttacgctttc atattttgat atattttgtg aaatgtggct g 101

<210> 154

<211> 101

<212> DNA

<213>Artificial sequence

<400> 154

tgctagcgat ttcctatgtt acatgatata ttaggattcg tattcagatg aggatatgtt 60

aacaaataga agttgaaaat caagtgtatt caggactaat a 101

<210> 155

<211> 101

<212> DNA

<213>Artificial sequence

<400> 155

gtgaggtcca tcgagttctc cagtgaggag attttttatt caaatctctt tggaagagtg 60

gtaatcacct tctcaaccac tctgttgtca ctgaaatcct c 101

<210> 156

<211> 101

<212> DNA

<213>Artificial sequence

<400> 156

accagtctct gacactcata gatttcaagt gtctgcaagg attgaaggat ggttggcaac 60

cttcccagca atagaggaca ttctctgatt gaaagaaaac g 101

<210> 157

<211> 101

<212> DNA

<213>Artificial sequence

<400> 157

gagatcaata gtaaaaggaa gaaaattcac ataaaatgtt tgattcccaa tcttcaaatt 60

tgtatacaac tctattctat gtattttcct tttttgggtt g 101

<210> 158

<211> 101

<212> DNA

<213>Artificial sequence

<400> 158

gatatcttct atctttatag tgacgaaatt gttttgatat gatttcagtc gtgtaatgtg 60

aaaggcgaaa tgcattgagg aaagagtagg aagattgata c 101

<210> 159

<211> 101

<212> DNA

<213>Artificial sequence

<400> 159

tttgtacaag ggttttcgat gatagctcct cctaatgcca cggttgttat agaaagatgt 60

gaagtttgaa tggaccgata aatgtcagca gaaatttgac a 101

<210> 160

<211> 101

<212> DNA

<213>Artificial sequence

<400> 160

atggttttct cctgccacct ttccgacaga gctgagctga caaaataata agtcactcaa 60

agcttttgat gaaaaccatg caaatcttta tacctcaaat g 101

<210> 161

<211> 101

<212> DNA

<213>Artificial sequence

<400> 161

tcgtttacat aaaatcagat ttacaaaata ttccttatca accaatttag aaaaaatgtg 60

acgctgccga gacttccgta taccgatcca gcttcagaac a 101

<210> 162

<211> 101

<212> DNA

<213>Artificial sequence

<400> 162

tcaaatgata atatgcagca gagctgacat atatgtcgta ctgtacctta cggtaaatcg 60

ctgtacagac atgttatagt tgaaactgct aggacagatc a 101

<210> 163

<211> 101

<212> DNA

<213>Artificial sequence

<400> 163

ttatgacagt taattatttt tatgactcga tttagtggtc ccgaaaccat ttcccgacta 60

gggtcaaatt aggggtgtca caaatctagt ccaagagttt a 101

<210> 164

<211> 101

<212> DNA

<213>Artificial sequence

<400> 164

actcgattta gtggtcccga aaccacttcc cgactagggt caaattagga gtgtcacaaa 60

tctagtccaa gagtttattg aaatccacag gcaaaaattc t 101

<210> 165

<211> 101

<212> DNA

<213>Artificial sequence

<400> 165

atatatggta tccgctacat aatttagtat gcatgagata gttaaaaatt tatgtttaaa 60

agaatagcgt actccatgct agtatgtaag tgaaatgcat g 101

<210> 166

<211> 101

<212> DNA

<213>Artificial sequence

<400> 166

gaaggaatgg atggaggaat aaggaagatg aaattcctat tagatattgt catacggtgt 60

tgttgagttc aaaccataat gtgcgatact ccgggctttg t 101

<210> 167

<211> 101

<212> DNA

<213>Artificial sequence

<400> 167

ccatagctcg actatggaaa ccaacaaagt ccattgggac actaaacacg gggtaattcg 60

ccaagacgat aatgatggaa aaatcttaga tatggaaata a 101

<210> 168

<211> 101

<212> DNA

<213>Artificial sequence

<400> 168

ctatggaaac caacaaagtc cattgggaca ctaaacactg ggtaattcgg caagacgata 60

atgatggaaa aatcttagat atggaaataa ggttaaaaat a 101

<210> 169

<211> 101

<212> DNA

<213>Artificial sequence

<400> 169

ttatgctaaa agattaatcg tcggtactcc aaatagacta atcggtaaaa aaggggttgg 60

atagaactca ttgacaagaa aatgtacaag aaagtgtact a 101

<210> 170

<211> 101

<212> DNA

<213>Artificial sequence

<400> 170

atatcattta cccatgtttt gggatatgaa ctccactgtt gtaaatgacg ctacatactg 60

cagatgtcgt acacccaacg cactagcttt cagtttctta t 101

<210> 171

<211> 101

<212> DNA

<213>Artificial sequence

<400> 171

gcgtttaaaa atgagcatta gcggcgtttt cagaaaacgc cgcaaaaaac ctaagcccaa 60

cggcgtcgtt ttcagaactt tcgtggtctt tagcggggtt t 101

<210> 172

<211> 101

<212> DNA

<213>Artificial sequence

<400> 172

tttcagaaaa cgccgcaaaa aatctaagcc caacggcgtc gttttcagac ctttcgtggt 60

ctttagcggg gtttttggat tagaaaaacg ccactaatga t 101

<210> 173

<211> 101

<212> DNA

<213>Artificial sequence

<400> 173

caaacgaaaa tcctcacaaa caactgaaag catttcatat ggtttgtcta agcatgaaac 60

ctcagggcgt aactgaggat caaatcaaat tgcgtgcttt c 101

<210> 174

<211> 101

<212> DNA

<213>Artificial sequence

<400> 174

aatactctat gagtttatat tacaggggat ttagcctgga ctggtaatct cactgtaagg 60

atgaggttcg cgggagtgtg ctctctgaaa tgaaatgtgt a 101

<210> 175

<211> 101

<212> DNA

<213>Artificial sequence

<400> 175

cgtcggcagg tggggttctt tatcagtagt cggtcacggg gctgaaaccc gcagcatcag 60

tccacgcact tcggtgatta caacgaaagc aagacgatag g 101

<210> 176

<211> 101

<212> DNA

<213>Artificial sequence

<400> 176

ataaaccaca aattaacatc acttaacatc ttcaaattat aggttttatg agctttactg 60

acaagctcat ggagcgtaaa gttgtcaaaa ttttgcatcc t 101

<210> 177

<211> 101

<212> DNA

<213>Artificial sequence

<400> 177

acaacaggat gaaagattcc agttggccag catgaccaat aaacaaatat aaggaacata 60

tcataggaga tgagcatgat aaaaatgtta tttctaatgg t 101

<210> 178

<211> 101

<212> DNA

<213>Artificial sequence

<400> 178

agcttcaaaa atctgttgca aaaacgtcgg tctagctttt cattcaacct aaattaaacc 60

gtcactaatc aacgtcaatc atgtgttcaa agctcacaaa g 101

<210> 179

<211> 101

<212> DNA

<213>Artificial sequence

<400> 179

tgaaaatcat tgagtatatg tgtcattgat tatatttttc ccctttttgc tattttcggt 60

cttcattcac tgatggatat tgctcttata gttggtggaa g 101

<210> 180

<211> 101

<212> DNA

<213>Artificial sequence

<400> 180

ctttgttgag ttattttctt gatctctcga ggagagactt aggataaaat ggaaccaata 60

acccatttct ttaatactaa aaaataaatt gaacttccaa a 101

<210> 181

<211> 101

<212> DNA

<213>Artificial sequence

<400> 181

tggcaaggag tacacttcag aaacctttaa taggttttgt gaagaggctg gaattgagca 60

tcagttgacg gcgccataca ctccacaaca aaatagagtt a 101

<210> 182

<211> 101

<212> DNA

<213>Artificial sequence

<400> 182

agaaacccag aaaatcaaaa tcaagttcaa acccttagta ttatttacta aaactataca 60

taccctaaaa tagtagtacc cttcccagca acacctcggg c 101

<210> 183

<211> 101

<212> DNA

<213>Artificial sequence

<400> 183

tgcaacatta gatgtattta tacggctact attaaaataa aatttagaag gagaacatta 60

aaaatatata tttctctgta ccttctgtgt ttaactgttc a 101

<210> 184

<211> 101

<212> DNA

<213>Artificial sequence

<400> 184

tttcctagca aagaaattga tgaaggcaag cttatgccaa ccaaatatag gactttaaga 60

tttttcgttt ccttaaaaaa ggttgttggt attttcatcc g 101

<210> 185

<211> 101

<212> DNA

<213>Artificial sequence

<400> 185

ttcctcaata tccaatcctt gcacttggag ttggaacact tcttccagtt aagcacaatc 60

agtcactttc aatttctcta atctttggaa cactcccaaa a 101

<210> 186

<211> 101

<212> DNA

<213>Artificial sequence

<400> 186

gcacaatcag tcactttcaa tttctctaat ctttggaaca ctcccaaaaa aaaaggtgaa 60

aagatgttca acaacacatc acattcctta acagtcaact c 101

<210> 187

<211> 101

<212> DNA

<213>Artificial sequence

<400> 187

agtcaactct tttagcatag aaaaagaatt cgtatgaagt tgaccgtccc atatcctctt 60

tgcattcctc aaatggggga ttgtgatttt cttcaagttg g 101

<210> 188

<211> 101

<212> DNA

<213>Artificial sequence

<400> 188

ctgcaacgag agagatggga cttattcctg agttcgtgta ggcatttgga ttgatgggtt 60

ttcttttata ttttaggcac caccatcttg gatgccatta g 101

<210> 189

<211> 101

<212> DNA

<213>Artificial sequence

<400> 189

gagcatagac acctcaagaa taataattca ctgtctgaaa gtccaatcaa ttattcaaaa 60

gatgacactt caaaaactta gatccagtca acatatatag a 101

<210> 190

<211> 101

<212> DNA

<213>Artificial sequence

<400> 190

agcttgatag tctgatcttt accgtttgat atgaagtaac gaccatccca acgactgtcg 60

aggaatgtaa taccttccag gtgtcccacc aaaactcctg c 101

<210> 191

<211> 101

<212> DNA

<213>Artificial sequence

<400> 191

ggtccaacca ggactcatga tgtgtcgata catttgaaaa ttgttcatta taaccactgc 60

ctgtaagata atcattggca gaagaagctc agatatatta t 101

<210> 192

<211> 101

<212> DNA

<213>Artificial sequence

<400> 192

agctgtgcaa cgtgcagctc gtaagaaacg aggtggactt gatggccgag gtttagctac 60

tgttgctgtt gtaggataca caaatgctgt aggtggccaa a 101

<210> 193

<211> 101

<212> DNA

<213>Artificial sequence

<400> 193

acttgtactc ttccttggag atcttaccat ctttgttggt gtcgaatttt tcgaacaccc 60

atttcatttc ttccacattg ggctgataag ttcttgaaga c 101

<210> 194

<211> 101

<212> DNA

<213>Artificial sequence

<400> 194

acaacaatgc ttccacacct tgcatatagg tctatgaggg cattactaat taccacatct 60

ttgtcaaagc ccttgcatat gatataggcc atcacagaat c 101

<210> 195

<211> 101

<212> DNA

<213>Artificial sequence

<400> 195

gacaacatgc tgcacatggg caataaattt agtatactta cttgatttga tctttggttc 60

tccttcacca tttgagcaaa caagactaga acatcatttg a 101

<210> 196

<211> 101

<212> DNA

<213>Artificial sequence

<400> 196

gagcaggcag aaaggatgct aacaattgaa attgcatcag gactgaaccc ttccaattgc 60

attttgcgaa atgcaaccaa actttcatcc ggtagtccat t 101

<210> 197

<211> 101

<212> DNA

<213>Artificial sequence

<400> 197

tgtgcataca ccggtcgtcg atacggcgtc cgcaaatgag tcaggcacgc ggacaagttc 60

cacatcaaag ctttgataac tttcatgcat aaatacatat t 101

<210> 198

<211> 101

<212> DNA

<213>Artificial sequence

<400> 198

acaggtgttt gagatgtctg caacagctct ctcatgaata ctccctactg aagaacaagc 60

ttcaaacctt attcctggta agtgttgctt cccgttattt g 101

<210> 199

<211> 101

<212> DNA

<213>Artificial sequence

<400> 199

gtcatatgct ttgctcgtgt caattttaag gcaaacgatc cttttctttc taaatgagac 60

cttaaaacga atgcaaaatc tcgtaagcca aaatcacatt c 101

<210> 200

<211> 101

<212> DNA

<213>Artificial sequence

<400> 200

cttcgggcac gagtctatag tgtggccata aagttcacaa cgaaaataga cattgggtaa 60

agactcatat tccactctcc gtgtttgtcc atcaatcttt a 101

<210> 201

<211> 101

<212> DNA

<213>Artificial sequence

<400> 201

tttgaaaaca ccgaggctcc ctttagttga tcaaacaaat catcaataca cggtaacgga 60

tatttattct ttattgtcac tttattcagc tgacgatagt c 101

<210> 202

<211> 101

<212> DNA

<213>Artificial sequence

<400> 202

tttcttatgt ttatagagtt ttagagattt gctcgggtta gaagtcgcag gagaccttat 60

tacactatct ggtcattact tcggtaaata aaagctttaa g 101

<210> 203

<211> 101

<212> DNA

<213>Artificial sequence

<400> 203

atctatcgca tctttaagaa aaggcaaaat cacaagttct cgaaataccg aaattttagc 60

atttcggctt ttcggcgaat attaatctaa gctactacaa g 101

<210> 204

<211> 101

<212> DNA

<213>Artificial sequence

<400> 204

gtgcatgtct aggattggat ccgaagggag cttggtactt aagcagttcg acagactcac 60

ctcctcttct tcctggtttc ctacctggtg cacagcttct a 101

<210> 205

<211> 101

<212> DNA

<213>Artificial sequence

<400> 205

ccatcgtatt cgctttgaaa atatggcgac attatttatt tggtgagaag tgccatgtat 60

attcggatca caaaagtctc aaatatttga tgactcaaaa a 101

<210> 206

<211> 101

<212> DNA

<213>Artificial sequence

<400> 206

ttgaaaatct atgcatgttt gagatgatta gttcaaactc tactgatccc atagattaaa 60

cttagaattt tgaggtttgc aagaggtaag taccttgaaa t 101

<210> 207

<211> 101

<212> DNA

<213>Artificial sequence

<400> 207

gttaaaccct tttactcgtt ttcacttaaa accgagtagt acaagctgtc taacataatt 60

taaaacgtca tattctatca taaaacacca aaatacacaa a 101

<210> 208

<211> 101

<212> DNA

<213>Artificial sequence

<400> 208

acaaaatcga gatgaaagaa gatcagacgt tgcactgaat ctccgtatcg tctcgtccta 60

ctctacttta cttttactag gcattgatta gtagctactc a 101

<210> 209

<211> 101

<212> DNA

<213>Artificial sequence

<400> 209

aatcgagatg aaagaagatc agacgttgca ctgaatctcc gtatcttctt gtcctactct 60

actttacttt tactaggcat tgattagtag ctactcatta c 101

<210> 210

<211> 101

<212> DNA

<213>Artificial sequence

<400> 210

aacaacaacc caaatcgcat ccttcttact tgctgacaat ggcagtccga acacaaagtc 60

cattgtgact cgatcccatt tccactcggg tatcatgatc g 101

<210> 211

<211> 101

<212> DNA

<213>Artificial sequence

<400> 211

tttaacctcg tatgataaga tgaagcatct tgtggttaaa cccgactctt agggagaggg 60

tctcattgtt cgtgggagac aagatcggaa tgctgatgat g 101

<210> 212

<211> 101

<212> DNA

<213>Artificial sequence

<400> 212

ttattttaga tcaataccaa taataacctt aagttttcaa ttattaaatt catatatcac 60

ttaccaaccc tcgttacacc ggagaacgtc ttatggatat g 101

<210> 213

<211> 101

<212> DNA

<213>Artificial sequence

<400> 213

gaatttgatg ttattttggg tatggattgg ttgacattgc atgatgctat aataaattgc 60

aaagaaaagg ttatagaatt aaagtgtgga aatggtgaaa c 101

<210> 214

<211> 101

<212> DNA

<213>Artificial sequence

<400> 214

tcatccggaa atactacagc agcttgatct gcttcttcat acttcatcca gataacttat 60

gttgcatggt ctgctccttg gttgctttat ctgctgcatg c 101

<210> 215

<211> 101

<212> DNA

<213>Artificial sequence

<400> 215

gcaatagcat taagtcagtt agaaaacaat aaccctttac catcagtggg caatttttac 60

aaattttatc cactataaca tgttggccca ggggatttga a 101

<210> 216

<211> 101

<212> DNA

<213>Artificial sequence

<400> 216

aacaaattct cttaactcta ggatgcccac aaacactcga atgttttcaa tgaggccatt 60

ctcaaacctc ttacacatag tagcttcagt ggatacatat t 101

<210> 217

<211> 101

<212> DNA

<213>Artificial sequence

<400> 217

ttctcttaac tctaggatgc ccacaaacac tcgaatgttt tcattgaggg cattctcaaa 60

cctcttacac atagtagctt cagtggatac atattctcgc a 101

<210> 218

<211> 101

<212> DNA

<213>Artificial sequence

<400> 218

cttttaggga tttcggcttt tcctaaaata tgtaagaaga ctaagtttta gagaagactt 60

accaccgatt ccttagtgaa tgattccaat gagtttccca c 101

<210> 219

<211> 101

<212> DNA

<213>Artificial sequence

<400> 219

taatgtgggg ttttcggctt tttgcaactt gtttttagaa aggtaaaaca agagggtttt 60

gctatggtct tgtcgtcaaa acttgaagtt ttagagggct g 101

<210> 220

<211> 101

<212> DNA

<213>Artificial sequence

<400> 220

accatttcaa caacaatatt tttcatagat atgttcatca taaaaacaat agaaattaaa 60

aagaaaggga acagaaagca aatccagtgt ttctctgcgg c 101

<210> 221

<211> 101

<212> DNA

<213>Artificial sequence

<400> 221

cagtcatcga agaaatttag agatgatttg ggccgatcta gagacacttt gggtttttct 60

agacgagatc gcgatcgacc ccctgtgagt gcacgagtca c 101

<210> 222

<211> 101

<212> DNA

<213>Artificial sequence

<400> 222

aatattaaaa gaagacccca taaagcctat acccatattc ggccatctct ctaaattata 60

gggtttcggc ttttggccaa cagttactct aggaatcgtt t 101

<210> 223

<211> 101

<212> DNA

<213>Artificial sequence

<400> 223

ggttaaagtt ctaagaagga agtaggttcc gttggcaaaa gtactatggt gtaatcatgg 60

aagggaagaa gctacttggg aatcagaaga gactatgcgt c 101

<210> 224

<211> 101

<212> DNA

<213>Artificial sequence

<400> 224

ttgtggatga aaagcggtgc taaaatgcag cttggtaccc aaagcttctg gcaatttctt 60

ccaaaatcgc gaggtgaatc tcggatctct atccgacaca a 101

<210> 225

<211> 101

<212> DNA

<213>Artificial sequence

<400> 225

agcattgatc agcgatcgtg gaacgtattt ttgtaataag gtaatcgaca cacttttgaa 60

aaaaaagggg tagtgcaacg agttgctacg acttatcacc c 101

<210> 226

<211> 101

<212> DNA

<213>Artificial sequence

<400> 226

tgtacgtgca tgataaactt gattctttgc taaatgaata gcactttgat tatcacaata 60

cacgttaata tgctcctgga ccaatcccaa ggttttagtc a 101

<210> 227

<211> 101

<212> DNA

<213>Artificial sequence

<400> 227

aaaacgatgt ttactccctt aagtttgttt gatgatgatg ggattttgga tgagttgcaa 60

gttaagccat cttggttgga tgagattaag agtaagcagc t 101

<210> 228

<211> 101

<212> DNA

<213>Artificial sequence

<400> 228

tatacattca tggcatacat catacagatt taattcatca tgcttttcat acaaaattaa 60

ccctaggggt ataaaggtca ttttgcatac ataggggtat t 101

<210> 229

<211> 101

<212> DNA

<213>Artificial sequence

<400> 229

gcagaatgga aggaagaaat tagcaatatc aagggaggaa tggagtttta gaagggaaaa 60

gcaaagaaag aggaggaaaa ggctgcgcgc gctgtgatag a 101

<210> 230

<211> 101

<212> DNA

<213>Artificial sequence

<400> 230

aaggtccatc gccttcgatc tatttcccta ctgcttaggg ggttaagatc tgtaaattca 60

gcctgttacc ctactgctta ggggtttaag acttgtcatc t 101

<210> 231

<211> 101

<212> DNA

<213>Artificial sequence

<400> 231

gaaccaatca ttcatcacgc tatagaaagc ttgtctagct ccatcattcg gattactagc 60

attaggttga gagtccgccg gcgctgtctc ttgtgcggga g 101

<210> 232

<211> 101

<212> DNA

<213>Artificial sequence

<400> 232

cgaggtagca ctcaagctgg atctgcgtca tctgaacaca taccagctga cgaggtacgt 60

ctaccaccgg ttaatgaaaa tgagccgtat gaccgggccg c 101

<210> 233

<211> 101

<212> DNA

<213>Artificial sequence

<400> 233

attatgtgat aaggccgaat ggccaatgtg atggatgtga aagtgtataa atgtgataag 60

gccgaatggc caatgtgatg aatgtgaaag tgtataaatg t 101

<210> 234

<211> 101

<212> DNA

<213>Artificial sequence

<400> 234

ccttcggggt tacttcagcc aattactata ccagaatgga aatgggaaac aatcactatg 60

gattttgtat cggggttacc tttgtctctg aggaaaaaag a 101

<210> 235

<211> 101

<212> DNA

<213>Artificial sequence

<400> 235

acagtaggcc agaatacttg tcattattct gctataattc tccttacatt atattttctc 60

atgttcatgt ttttggttca acacttttct catgttaata t 101

<210> 236

<211> 101

<212> DNA

<213>Artificial sequence

<400> 236

aaatgaatac attcagtatg ttaagtgggc caggtacgca ttatgtactt atatgtgagt 60

ttgatctgca gtgaatcata agtgtgcata gtgatacacc g 101

<210> 237

<211> 101

<212> DNA

<213>Artificial sequence

<400> 237

tacttgttct tattcttaat tgtgagattt aatccttgtt ttaatatttt aggatattaa 60

ttcaggtttt gatgtgctta ttcagttgag caaaagtccc t 101

<210> 238

<211> 101

<212> DNA

<213>Artificial sequence

<400> 238

catcctgctt aaagcgtttt tgacactatt tgcttagaaa catctactcg aaattatttt 60

ttcaagagca attgtagcaa aagcgcgtcc acgtgggagc t 101

<210> 239

<211> 101

<212> DNA

<213>Artificial sequence

<400> 239

atataaattt cctcttccaa atcaccatgt aaaaatacag tcttcacatt gagctgaact 60

agttcaagat catattgcgc aaccaaggct agcaaaatcc g 101

<210> 240

<211> 101

<212> DNA

<213>Artificial sequence

<400> 240

cgtattaagg actttttgag tgtatatgaa tgatgttatg attaattgag gattgtatgt 60

gaaatgattt tctaaattat gagataagtc tggtaatgct c 101

<210> 241

<211> 101

<212> DNA

<213>Artificial sequence

<400> 241

ggtagctacg gtggtggtgg taagtcctct ggtttattat tgttaatctc attaaatagt 60

gggctatctc acggtgggaa atttcctaca gcaggtggtt t 101

<210> 242

<211> 101

<212> DNA

<213>Artificial sequence

<400> 242

taaatgtgta acagccttga attgtaacca ctataagcta aaaagtcaca tgcaataaac 60

acattaaaat gagtttatta aacaaatgaa aacacttatt a 101

<210> 243

<211> 101

<212> DNA

<213>Artificial sequence

<400> 243

aatataaatt tgaagctttc tcttccatct tttgatgttc ttctgacttc tgctgaacaa 60

ccacctcgga ggcaatactc ctactgattt gatcgccaac g 101

<210> 244

<211> 101

<212> DNA

<213>Artificial sequence

<400> 244

tttcttcatc tactaaaaag aacaacacaa ttttagcttt agtgacaatg tttcttcatt 60

tctaataacc tcccttctta tcttgctctt catgttatca g 101

<210> 245

<211> 101

<212> DNA

<213>Artificial sequence

<400> 245

tgttcgatcg gtaagggtta gttattggac gttccataac taatttacat aaggaagagt 60

tggtgtccga ggcatccttg gtagctataa ctagcttatt g 101

<210> 246

<211> 101

<212> DNA

<213>Artificial sequence

<400> 246

aaagtcacgg aatgggtaag tcgccaatcc ctaaaacgga ctatgtaatg tactttatcc 60

tctgggttac gggggggcat tttaccagag gtttatattg t 101

<210> 247

<211> 101

<212> DNA

<213>Artificial sequence

<400> 247

ttccgaccct tcttcactaa aaattaatta tctctttgta caaaatttgt atgatgtttc 60

catttgttta tattgaaaat atactaattc aacattttaa a 101

<210> 248

<211> 101

<212> DNA

<213>Artificial sequence

<400> 248

ccctaaactt taaactccaa atccaacctc gaacctcaaa tcctgaactc gaaccttgaa 60

tcttgaactc aggtttcggg atttgaggtt tggggttcag a 101

<210> 249

<211> 101

<212> DNA

<213>Artificial sequence

<400> 249

ggtactgaag cttcctctcg agcacgaatc acataagccc tagctagtgg ccatgcctta 60

gatctaacag cagaatcttt cattccacct caattgttac c 101

<210> 250

<211> 101

<212> DNA

<213>Artificial sequence

<400> 250

ctcaacagtt tagtataaag cacttgagag aaactaaatt tgttctagac aaaaggatcg 60

taagaacaaa aggatagcat tatcacaagc ttttaaaaca t 101

<210> 251

<211> 101

<212> DNA

<213>Artificial sequence

<400> 251

tttcactctt atatcttgtg tttctgtctt ccatgtttta cttacgtatc gaaattgtaa 60

caatcaaatg ttagctcatt gattgtctaa gtttaaactg a 101

<210> 252

<211> 101

<212> DNA

<213>Artificial sequence

<400> 252

tagtctttat tgattactac tccatatatg ggtattttac ctgatgcact gcaaaagtga 60

aacctttgat aaattcaaag agttctgtgc gaaagtggag a 101

<210> 253

<211> 101

<212> DNA

<213>Artificial sequence

<400> 253

gctttatgtc tccatctgtc taaatacaaa aatctctcat cttagttgat ctgctttgtt 60

gattgggaaa aacttctgca agaactttcc ggtgagttca t 101

<210> 254

<211> 101

<212> DNA

<213>Artificial sequence

<400> 254

gcagtggagc tggttaaaga tggtagatct tgccttcctg cattaacagc gaagcagatc 60

gaaaacaaag tcttgcctcc atcggttgca gtggagctgg t 101

<210> 255

<211> 101

<212> DNA

<213>Artificial sequence

<400> 255

ccaagacgac aagacgaagg cagcccctca acgaggccca gacattcact cggctcatca 60

tcagcggcca tgcaatcacc aggcctaacg agagcaccga c 101

<210> 256

<211> 101

<212> DNA

<213>Artificial sequence

<400> 256

tatacaccca cttaacacgt atgaccttct tgtgttctgg tctgttcacc aattcccatg 60

tctgattctt gtcaatcatt tccaacttag cctccatagc t 101

<210> 257

<211> 101

<212> DNA

<213>Artificial sequence

<400> 257

cttagctaac cgctgcttaa gcattgtagt acgatgagac ctacaagctt taatgccctt 60

actctagtta cccaaatcaa catccgtttg tttcaatttg g 101

<210> 258

<211> 101

<212> DNA

<213>Artificial sequence

<400> 258

agaacccaac cagttttgaa acggaacctc caagagcgcc ctatataagc catgtgggtt 60

gtatctaaca atatcaatgg cagtaatcag aaaaaactgt g 101

<210> 259

<211> 101

<212> DNA

<213>Artificial sequence

<400> 259

aacccaacca gttttgaaac ggaacctcca agagcgccct atataaggcg tgtgggttgt 60

atctaacaat atcaatggca gtaatcagaa aaaactgtgc g 101

<210> 260

<211> 101

<212> DNA

<213>Artificial sequence

<400> 260

ggcatgtggg ttgtatctaa caatatcaat ggcagtaatc agaaaaaacc gtgcggaagg 60

tgttgcaaag aataattagg aaactcatcc ttctattcag c 101

<210> 261

<211> 101

<212> DNA

<213>Artificial sequence

<400> 261

tctattcagc attagcaagc ccactatcaa gcctttctcg gatattattg ttcgacaatc 60

tgcctcattc ccatgtgaac cagcttccag agtatgcaat a 101

<210> 262

<211> 101

<212> DNA

<213>Artificial sequence

<400> 262

ctattcagca ttagcaagcc cactatcaag cctttctcgg atattattag tcgacaatct 60

gcctcattcc catgtgaacc agcttccaga gtatgcaata t 101

<210> 263

<211> 101

<212> DNA

<213>Artificial sequence

<400> 263

tattcagcat tagcaagccc actatcaagc ctttctcgga tattattatc cgacaatctg 60

cctcattccc atgtgaacca gcttccagag tatgcaatat c 101

<210> 264

<211> 101

<212> DNA

<213>Artificial sequence

<400> 264

gaaataagca tgatctggtc cctctccacc gcttggcata gtctgtcgag taaaattaag 60

ctttttcgga aaagccaagt cttccttcca aaatcagttc c 101

<210> 265

<211> 101

<212> DNA

<213>Artificial sequence

<400> 265

ccctaaagtt gtctgtgtta tacagctttt ctgtccaaac cgtacatata aatgtaggtt 60

tggaacttgg agccaccatc gagcctttga cagataactg a 101

<210> 266

<211> 101

<212> DNA

<213>Artificial sequence

<400> 266

aagaataaat taggttttgt tggtggttcc tattctaaag aattgttgcc agtcgatttg 60

tatccacaat aggatagata taattctcta gtgttgtcat g 101

<210> 267

<211> 101

<212> DNA

<213>Artificial sequence

<400> 267

tgattaaata tccatagagc acattgggac aaactctatg atagtgcata tgctgacaaa 60

aggtttacca cctaaagtct tttatgagta cactgctcat a 101

<210> 268

<211> 101

<212> DNA

<213>Artificial sequence

<400> 268

gcttctactg ctccgttcat cttcgggcgg tagggtgaag aattatgata ctttatctgg 60

aattgctcac atacttcttt catcatcttg ttgttcaaat t 101

<210> 269

<211> 101

<212> DNA

<213>Artificial sequence

<400> 269

cttagcattt tgtcatatgt gtcgtattag ttagccaatg tgatggctcg ttttggtata 60

ttattccatt ttgtatatgg ccatgaaaca tggctcattt t 101

<210> 270

<211> 101

<212> DNA

<213>Artificial sequence

<400> 270

cgtaacccta ttccgacatt gaatatgggt gaggggtgtt acaagtctgg ccttataagc 60

ttttactatt ttatttgcat ttcttttctt tttatagatc t 101

<210> 271

<211> 101

<212> DNA

<213>Artificial sequence

<400> 271

tgggtcagat aatttttctc atgtggcttc agttgtctcg acgcataagc tacaactcga 60

ccctcttgca tcaatacgca acccaaccca agtagggatg c 101

<210> 272

<211> 101

<212> DNA

<213>Artificial sequence

<400> 272

tcgcgtaggt tatcactact cgaagtttat caaggttttg tgactgatga gctacaatgc 60

cttctcgctt gactgatttt tctgaagaaa catttagcca g 101

<210> 273

<211> 101

<212> DNA

<213>Artificial sequence

<400> 273

caaagtccgt cccaaaataa taaagatggt gcatgtgaga gtcaagtttt agagggcgtg 60

ttagggaaat tgcacatgca atatggtagt tgacgccaca a 101

<210> 274

<211> 101

<212> DNA

<213>Artificial sequence

<400> 274

agtgaaacat aattgaaatg atccaccgcc tcattaaaaa gataaaaaga aaaaaaaaaa 60

gcatttactt acgatgaggg tattgaaatt ccatcttgct t 101

<210> 275

<211> 101

<212> DNA

<213>Artificial sequence

<400> 275

gtccaacggt tttaaagtgt taatttgaga tctgtaagag ggtttctgta gtgctgaatt 60

atgtgtggta ttttttatat tgcggcaggg ggggcatgtg g 101

<210> 276

<211> 101

<212> DNA

<213>Artificial sequence

<400> 276

cagtatatca catcctctat gagatatgaa gcaattttac ccaaaaatca atttgaatgt 60

aaaatcaata attacaactt cgacttgtac ctgagaatat a 101

<210> 277

<211> 101

<212> DNA

<213>Artificial sequence

<400> 277

gaattatcca gtatccactc atgaaatttc tgatttctct ttggatgtaa agggagatgc 60

tgacagggct tgagtcagag tgaattgttc tccttttcaa c 101

<210> 278

<211> 101

<212> DNA

<213>Artificial sequence

<400> 278

attcatcagc tagcaactca gttctttcaa acaaatcccg aaactataaa atcaacccac 60

cgaaatcacc aaatgcaaat ttcaaatcac acagttcacg c 101

<210> 279

<211> 101

<212> DNA

<213>Artificial sequence

<400> 279

aatcgggctg ctgtttattt ggaaatgggc aaggtatggt tatttatttt tatttgttta 60

gaagctacta taaaatgtac ttctttcgtt gtagtttttg c 101

<210> 280

<211> 101

<212> DNA

<213>Artificial sequence

<400> 280

agctcaaaag ctgaacagaa gctcgtaaga gctaaacaaa aactcataat ggttgagcag 60

aagctcataa gagctgaatg gaaactcgta taagttaaac a 101

<210> 281

<211> 101

<212> DNA

<213>Artificial sequence

<400> 281

tcaatggaga aggattccat atttgggttg tcaaaatgaa gacttaccta caggcattcg 60

acctatggga ggtagtcaat tcagatgcag agccagcacc t 101

<210> 282

<211> 101

<212> DNA

<213>Artificial sequence

<400> 282

tgattcgtta tcacttacca tgaagaaatt gatgctacta tctatgtggt ttaataagtc 60

atttaaataa tatcagcttg cagttgtttt cctttgatat g 101

<210> 283

<211> 101

<212> DNA

<213>Artificial sequence

<400> 283

tgctcgattt gtggaaatgc tgcaggttgc tgtggcttct ggttgagctt ctccaaattg 60

aaggcaacgt ctactcatgc tgcgttggag ttcactggcc t 101

<210> 284

<211> 101

<212> DNA

<213>Artificial sequence

<400> 284

aataatgagc tgtaataaac ctgactaacc tttttacaaa tctgagcaac ctctttctga 60

accatttggt atatatctga agacttatat gacttcacca g 101

<210> 285

<211> 101

<212> DNA

<213>Artificial sequence

<400> 285

agggaagtca tccaatcttg gagtaggtaa tgtagagtca ctagttttct ttcatccccc 60

tactagcaag gcaacctcaa ttgctactgg cctcaccatg g 101

<210> 286

<211> 101

<212> DNA

<213>Artificial sequence

<400> 286

ttgttttcag gtttacaatt cactgtttcg ctctgtgttt gaaatctatg gatatgggtc 60

tgtctgtttc tttttggttg ctttttcgga gactgatttc a 101

<210> 287

<211> 101

<212> DNA

<213>Artificial sequence

<400> 287

ttgaaaacgc cttttcgtac cattcatgag gtaagacctc tttgtcggag tcccttgtgc 60

agtgatataa cattgcatta aaatgcagag caccatttag g 101

<210> 288

<211> 101

<212> DNA

<213>Artificial sequence

<400> 288

tggctgcgtt cagtgtgcct cttgaccaaa gtgatgatga agcagtaaca gcattgtgta 60

ttgaaggctt ccgctgtgca attcatatca ctgctgtgat g 101

<210> 289

<211> 101

<212> DNA

<213>Artificial sequence

<400> 289

atgatgaagc agtaactgca ttgtgtattg aaggcttccg ctgtgcaata catatcactg 60

ctgtgatgtg catgaagact catagagatg catttctgac t 101

<210> 290

<211> 101

<212> DNA

<213>Artificial sequence

<400> 290

aacaggtgat catgctttca ttttcaacgt ggcttgcatg gtttttagcc ggaaagctcc 60

atggttaccc tgaatcatgg ataccatctt caatggatag t 101

<210> 291

<211> 101

<212> DNA

<213>Artificial sequence

<400> 291

ctgttatccg atcaaaatta tctaaatccc aaaaatcctt ttttttttgt tgctcaataa 60

gcagagttcg ggggtgtgaa aatggcttct gatgtgcctt g 101

<210> 292

<211> 101

<212> DNA

<213>Artificial sequence

<400> 292

cacttaggat gcaccctact ttgtgaggaa caagcaagat aaaatatacg aattggcctt 60

tgtttattat gcttatcatc ggccctaaca aggagtccca g 101

<210> 293

<211> 101

<212> DNA

<213>Artificial sequence

<400> 293

atgcacccta ctttgtgagg aacaagcaag ataaaatata caaattggcg tttgtttatt 60

atgcttatca tcggccctaa caaggagtcc cagtctaact a 101

<210> 294

<211> 101

<212> DNA

<213>Artificial sequence

<400> 294

cagagtatgc aatatccacc aaagtcatcc caatacttgt ctgaatgcca tcatttcgtt 60

ctcgtcccgt aaccgaccaa ccagcggttg cagggttgag a 101

<210> 295

<211> 101

<212> DNA

<213>Artificial sequence

<400> 295

atgcaatatc caccaaagtc atcccaatac ttgtctgaat gccgtcattc cgttctcgtc 60

ccgtaaccga ccaaccagcg gttgcagggt tgagaaaggg t 101

<210> 296

<211> 101

<212> DNA

<213>Artificial sequence

<400> 296

gtctgaatgc cgtcatttcg ttctcgtccc gtaaccgacc aaccagcggc tgcagggttg 60

agaaagggtg cggagaaggt tccaagagtc ggaacgaacg c 101

<210> 297

<211> 101

<212> DNA

<213>Artificial sequence

<400> 297

ttgcagggtt gagaaagggt gcggagaagg ttccaagagt cggaacgaaa gcgattatct 60

gtctataatt ttttataact gagagaagta ttgaaatggt t 101

Claims (9)

1. the SNP marker related to upland cotton fiber elongation, it is characterised in that the molecular labeling such as SEQ ID Shown in one of NO.1~SEQ ID NO.297；Wherein, undergone mutation at 50bp, cause elongate fiber rate polymorphism occur.

2. the SNP marker related with upland cotton fiber elongation combines, it is characterised in that including SEQ ID NO.1~SEQ Any two or multiple in molecular labeling shown in ID NO.297.

3. a kind of SNP chip, it is characterised in that the SNP chip is included shown in SEQ ID NO.1~SEQ ID NO.297 In molecular labeling any one, it is two or more.

4. test right requires the primer or kit of 1 SNP marker.

5. the primer sets or kit of the SNP marker combination of test right requirement 2.

6. application of the SNP marker in cotton fiber extension rate marker assisted selection described in claim 1.

7. the SNP marker described in claim 1 is in the early prediction of germplasm resource for cotton elongate fiber rate or screening Using.

8. the SNP marker described in claim 2 combines the application in cotton fiber extension rate marker assisted selection.

9. the SNP marker described in claim 2 combines early prediction or the screening in germplasm resource for cotton elongate fiber rate In application.