CN111690733B - 一种激素性股骨头坏死易感基因panel - Google Patents
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Abstract
本发明公开了一种激素性股骨头坏死易感基因panel，一种激素性股骨头坏死易感基因panel，其为包括下述587个人类基因组位点的基因集。本发明提供的基因检测panel可用于激素性股骨头坏死易感的诊断预后，具有基因覆盖度广，可移植性强，适用于新开发的检测平台。
Description
技术领域
本发明属于基因检测领域，具体涉及一种用于激素性股骨头坏死易感性检测的基因panel，还涉及该基因panel在基因检测装置中的应用。
背景技术
糖皮质激素因其抗炎、抗过敏、抗休克、非特异性抑制免疫等多种作用，被广泛应用于自身免疫性疾病、过敏反应性疾病、严重感染和器官移植后状态等，但激素性股骨头坏死(Glucocorticoid-associated osteonecrosis of femoral head，GA-ONFH)是其严重并发症。GA-ONFH好发于30-40岁的青壮年，往往起病隐匿，常在发现时即已发生股骨头塌陷变形，致残率高，对个人及社会带来巨负担，是骨科的难治性疾病。
然而，临床实践证明并非每一个接受糖皮质激素治疗的患者都会发生GA-ONFH，这一比例在接受激素治疗的系统性红斑狼疮病人中为0.8％-33％，在肾移植病人中约为6％，在急性淋巴细胞白血病或非霍奇金淋巴瘤病人中约为15.5％。虽然激素剂量被报道可能与GA-ONFH的发生存在相关性，但在实际临床工作中，接受相似激素治疗方案的患者，也并非均会发生股骨头坏死，这说明激素性股骨头坏死是用药因素和个体因素共同作用而导致的复杂性疾病。遗传因素作为最重要的个体因素之一，被报道与GA-ONFH的发生相关。随着分子生物学与遗传学的深入研究，越来越多的结果证实GA-ONFH具有极大的遗传易感性，但现有的研究基于候选单核苷酸多态性位点(SNP)分型方法，一次检测一个到数个位点，无法全面地反映GA-ONFH的遗传易感性，因此无法建立准确地、适用于临床实践的预测模型。
用于高通量的筛查基因组多态性位点的方法包括芯片(Microarrays)技术和二代测序(Next Generation Sequencing)技术。芯片技术只能筛查数据库已知的多态性位点，无法检测新发现或数据库未录入的多态性位点，开发拓展性能有限；二代测序技术，包括全基因组测序、靶向捕获测序、多重PCR靶向测序。其中，全基因组测序可以在全基因组水平上检测多态性位点，但测序数据量大，成本高昂；目标区域靶向捕获测序，例如全外显子捕获测序，适合大数据的靶向捕获，操作复杂且开发成本高；多重PCR靶向多态性位点检测是一种多重PCR和高通量测序相结合的高效检测技术，通过对多个待检位点设计特异性引物，利用多重PCR技术进行扩增，在保证扩增均一性的前提下，对SNP、Indel等几百甚至上千个多态性位点进行快速靶向线性扩增后，结合高通量测序技术实现对大样本多位点的检测，操作简单灵活，通过两轮PCR就能完成文库构建，适合中小通量的数据量。
目前，尚无用于预测GA-ONFH发生的基因集panel，也无二代测序方法在预测GA-ONFH中的应用。既往研究只基于对已有GA-ONFH发病机制的研究，对少数与激素代谢、血管功能、骨代谢及脂代谢等关键基因位点进行检测。但GA-ONFH是复杂的全身代谢性疾病和多基因相关疾病，目前对GA-ONFH发病机制的研究仍不清楚，既往研究涵盖的基因位点有限，需要二代测序方法进行更高通量的检测，以确定用于GA-ONFH风险检测的更优化的SNP组合。
发明内容
为了弥补现有激素性股骨头坏死易感性预测和检测技术的上述不足，发明人对已知的骨坏死及糖皮质激素代谢相关基因的进行归纳和分析，发现了许多基因与GA-ONFH高度相关，从而开发出一种新的激素性股骨头坏死易感性基因panel，该基因panel覆盖度广，能够用于从GA-ONFH风险评估到GA-ONFH的诊断、预后等GA-ONFH发生发展的各个阶段，并且可移植性强，适用于目前国内市场的测序仪器或新开发的检测平台。具体而言，本发明包括如下技术方案。
一种激素性股骨头坏死(GA-ONFH)易感基因panel，其为包括下述587个人类基因组位点的基因集：
rs710322、rs2294486、rs2294487、rs150880809、rs7407、rs144487103、rs12738235、rs10909811、rs78132938、rs11121552、rs9435659、rs761421、rs2076603、rs4920608、rs67232976、rs6666034、rs2064374、rs2295942、rs3748705、rs600674、rs12568483、rs74843031、rs10493845、rs78167983、rs4658219、rs147504208、rs79090643、rs6424379、rs11579366、rs2004659、rs9728345、rs2231375、rs2280474、rs4971066、rs17472444、rs7550383、rs10919082、rs4656656、rs4656657、rs7526721、rs10800420、rs3748618、rs2274227、rs3795503、rs10797850、rs1256930、rs10900525、rs11120575、rs1059704、rs2297545、rs2297543、rs2076149、rs9435867、rs7539016、rs7549137、rs7551511、rs2049182、rs10489691、rs3795375、rs3795374、rs963982、rs963981、rs4659948、rs562038978、rs111896385、rs1056104、rs7560262、rs58882124、rs200809231、rs187904341、rs937725、rs12233086、rs11691981、rs777432、rs12052654、rs13025791、rs13025959、rs2255161、rs11686946、rs35720878、rs2124971、rs2272499、rs16860497、rs2293649、rs56084828、rs2305414、rs1046356、rs2287600、rs13021295、rs59458664、rs3214826、rs2305541、rs2290464、rs2924811、rs2971863、rs1470864、rs2167885、rs78122518、rs72982319、rs143258246、rs838543、rs838549、rs78451449、rs151152774、rs79471829、rs117751285、rs200139615、rs3790993、rs200039497、rs456168、rs6796483、rs6777976、rs61743511、rs61743461、rs2070987、rs1546737、rs10933973、rs7614116、rs75144949、rs3732755、rs12492699、rs150242411、rs583550、rs35070271、rs55683908、rs843358、rs843357、rs11924016、rs371821065、rs6762208、rs1823238、rs6804448、rs138704337、rs28434055、rs322117、rs11355796、rs16844401、rs75501914、rs730420、rs2269877、rs62289296、rs62289297、rs4320134、rs3755863、rs8192678、rs11940551、rs6837020、rs77939156、rs4031826、rs2219471、rs3214870、rs3816584、rs2305946、rs7655964、rs1870377、rs7349683、rs3836713、rs861340、rs13128286、rs3966087、rs2175766、rs61758879、rs2241894、rs13147012、rs6847454、rs1039808、rs3756122、rs17024037、rs12650966、rs2289318、rs12648678、rs29675、rs1126417、rs162850、rs162848、rs332811、rs37368、rs4371784、rs3797209、rs1423099、rs10070440、rs423906、rs401302、rs3749669、rs59653731、rs10477486、rs6888031、rs11283943、rs2227950、rs2303076、rs2306960、rs3812036、rs70997617、rs3817064、rs3817063、rs75304543、rs368030749、rs372469036、rs778456436、rs1749158、rs2842949、rs6239、rs11754288、rs13204445、rs3749877、rs3749878、rs76902576、rs80125253、rs2297740、rs7762830、rs12435、rs1132742、rs1131123、rs1131500、rs1140546、rs1051488、rs3808343、rs117545108、rs4720537、rs4222、rs67361882、rs2286213、rs4721888、rs73705179、rs78155900、rs114097201、rs367830344、rs375743758、rs4646453、rs71540916、rs71540918、rs1424426、rs12671813、rs2278130、rs2069459、rs2069456、rs72494453、rs61013791、rs7829535、rs658948、rs2280335、rs35858677、rs145012061、rs34462909、rs17092126、rs1078363、rs6996616、rs2278467、rs1042381、rs10956412、rs17056759、rs6435、rs6397、rs12549574、rs11793555、rs7853758、rs2027433、rs3737309、rs2015408、rs2275137、rs10796042、rs2282383、rs6602051、rs7100510、rs1954181、rs12256835、rs2297329、rs2297328、rs11008968、rs148671234、rs138210257、rs10827116、rs2230396、rs2230395、rs2230394、rs2660169、rs735877、rs3736583、rs3736582、rs7912404、rs4986789、rs7894、rs4575219、rs3739968、rs6578504、rs11603496、rs3740620、rs11605894、rs11605072、rs398075678、rs96489、rs331510、rs2657167、rs10836954、rs11037444、rs60832895、rs11037445、rs16930982、rs16930998、rs55802517、rs12272856、rs1043388、rs1043390、rs1128396、rs2072651、rs10458926、rs10833050、rs10766524、rs2280331、rs369353409、rs574381、rs626670、rs2286163、rs200951888、rs57503021、rs7932320、rs7130258、rs66861805、rs7950735、rs291241、rs7931870、rs2186627、rs12790613、rs503223、rs1893764、rs79715120、rs79669172、rs77206991、rs79417294、rs114979979、rs75994039、rs75240316、rs59358830、rs57751948、rs775990209、rs2075626、rs58283839、rs2241281、rs2970827、rs2241280、rs2241279、rs12426675、rs1800692、rs11542844、rs2273986、rs7138535、rs4763232、rs7135018、rs12313469、rs10772423、rs12370363、rs78152338、rs2708381、rs2600357、rs2600356、rs2599404、rs2600355、rs11557132、rs61928643、rs9943714、rs9943809、rs2280446、rs6581565、rs825074、rs10861953、rs4072888、rs12821855、rs73166888、rs1451772、rs1817104、rs1669412、rs9315906、rs7982426、rs520342、rs3092904、rs12429818、rs7331894、rs1805097、rs2275841、rs2228036、rs1713417、rs2297612、rs4981349、rs74036552、rs150516189、rs1808975、rs2235962、rs3742885、rs181572、rs75391113、rs60593979、rs2180770、rs3837659、rs3829955、rs7153601、rs11626446、rs17127245、rs4401027、rs60585083、rs11366248、rs2260160、rs2467426、rs12441861、rs2277531、rs3759791、rs12702、rs2228368、rs3736180、rs8023508、rs12592155、rs28706938、rs28673176、rs2277598、rs57373048、rs17875502、rs397954069、rs28504011、rs11259927、rs2304796、rs3743262、rs10664668、rs4646626、rs3832984、rs12447306、rs2667661、rs2667660、rs2240691、rs2302607、rs115465506、rs1050113、rs14122、rs11865800、rs11076125、rs11076176、rs12691052、rs9928398、rs2241619、rs2241620、rs58353328、rs3803640、rs11557187、rs2077412、rs2663345、rs2286672、rs10999、rs3744400、rs16956647、rs11078698、rs76317718、rs148830167、rs61274670、rs2074274、rs2074273、rs2074272、rs111232194、rs2074146、rs4796030、rs2074519、rs3744372、rs2304967、rs143471015、rs1719152、rs16532、rs2227322、rs25645、rs4072639、rs2070833、rs756100328、rs79526563、rs2301682、rs143818567、rs2285524、rs4793639、rs3216895、rs2301627、rs2301625、rs3785925、rs2075555、rs2075554、rs2256835、rs2665799、rs2302133、rs2302134、rs10605889、rs7208382、rs11077414、rs9909216、rs12941264、rs690371、rs2467577、rs2070919、rs690514、rs2070918、rs1688149、rs492095、rs35286723、rs2306690、rs552432、rs2305027、rs2240900、rs7243349、rs17851892、rs7234849、rs3763951、rs713041、rs35870594、rs10421267、rs11671881、rs8120、rs2292151、rs7255265、rs2240745、rs2277969、rs35647251、rs137943075、rs7245563、rs2056820、rs56156262、rs36008189、rs12459147、rs34433978、rs3833221、rs305968、rs12975986、rs34650210、rs305975、rs73039860、rs1799782、rs17725124、rs2547238、rs45468391、rs8100114、rs4802905、rs12609379、rs12977654、rs56960413、rs862708、rs78628129、rs398059497、rs41275620、rs6133723、rs2295576、rs1078761、rs6030462、rs3746504、rs3737063、rs3918254、rs13969、rs140429049、rs8133052、rs1056892、rs11701124、rs8131313、rs8132521、rs35796750、rs881712、rs2839077、rs59769541、rs11464071、rs11089978、rs11089977、rs165657、rs361666、rs361762、rs362003、rs362124、rs362132、rs361611、rs361580、rs362011、rs361721、rs1476576、rs11089442、rs2074731、rs3218318、rs2072862、rs5758651、rs6006950、rs200564038、rs140313、rs372438307、rs376698610、rs6654760、rs2071776、rs2854412、rs6616890、rs143921252。
上述激素性股骨头坏死(GA-ONFH)易感基因panel可以用于构建基于多态性位点的GA-ONFH风险预测模型。
上述GA-ONFH风险预测模型选自下组：以OR值作为权重的遗传风险评分(an oddsratio weighted genetic risk score，OR_GRS)模型、可释方差遗传风险评分(plainedvariance weighted genetic risk score，EV_GRS)模型。
其中，在以OR值作为权重的遗传风险评分(OR_GRS)模型中，计算所有纳入模型SNP的OR值权重，该评分方法的计算公式如下：
ωi＝ln(ORi)，
上述OR_GRS模型又可以是基于OR_GRS风险评分的单因素逻辑回归分析模型和基于OR_GRS风险评分的多因素逻辑回归分析模型，其中，
基于OR_GRS风险评分的单因素逻辑回归分析模型的训练公式为：
logit P(D＝1|G)＝α+β(OR_GRS)。
基于OR_GRS风险评分的多因素逻辑回归分析模型是同时考虑OR_GRS、性别(Sex)、年龄(Age)、原发病(PrimaryDisease)进行的多因素逻辑回归分析模型训练，公式如下：
logit P(D＝1|G)
＝α+β1(OR_GRS)+β2(Sex_0)+β3(Sex_1)+β4(Age_0)+β5(Age_1)+β6(PrimaryDisease_1)+β7(PrimaryDisease_2)。
在可释方差遗传风险评分(EV_GRS)模型中，同时考虑SNP效应和风险等位基因频率(即最小等位基因频率MAF)，该评分方法的计算公式如下：
上述EV_GRS模型又可以是EV_GRS单因素逻辑回归分析模型或者EV_GRS多因素逻辑回归分析模型，其中，
EV_GRS单因素逻辑回归分析模型训练的回归公式为：
logit P(D＝1|G)＝α+β(EV_GRS)。
EV_GRS多因素逻辑回归分析模型是同时考虑GRS、性别(Sex)、年龄(Age)、原发病(PrimaryDisease)四个因素进行的多因素逻辑回归分析模型训练，公式为：
logit P(D＝1|G)＝α+β1(EV_GRS)+β2(Sex_0)+β3(Sex_1)+β4(Age_0)+β5(Age_1)+β6(PrimaryDisease-1)+β7(PrimaryDisease_2)。
上述GA-ONFH风险预测模型可以通过编程、通过数学软件包形式被输入计算机或者基因检测装置比如illumina测序平台，从而预测受试者是否属于激素性股骨头坏死易感人群，方便医生制定给药方案。
本发明的另一个方面在于提供一种激素性股骨头坏死易感性检测装置和配套的试剂盒，其包括用于检测上述基因panel中基因的引物或者DNA/RNA探针。
例如，用于检测基因panel中人类基因组位点的引物序列如表2所示。
上述的基因panel和引物适用于多重PCR靶向测序、GA-ONFH预测，通过检测生物样本中的基因组，可以进行GA-ONFH的风险预测。
本发明构建的基因panel在用于预测GA-ONFH风险时，具有如下优点：
1.属于首个预测GA-ONFH的基因panel。通过对目前公认的常用数据库中骨坏死及糖皮质激素代谢相关基因进行筛选，最终选择的基因覆盖度广，与GA-ONFH相关性高。该技术的开发并不局限于是否接受糖皮质激素治疗或是否患有骨坏死，是首个可靠的用于预测GA-ONFH发生的基因检测panel。
2.检测成本低，适用范围更广。本发明开发的panel适用于主流illumina测序平台，相对于目前其他二代测序技术检测方法而言，本发明的panel检测平台对基因的检测和分析成本更低，极具推广价值。
3.分析便捷，可移植性强，可操作性强。该技术中的信息分析形成的软件包能够安装于多个平台，从下机数据到各种变异结果一步即可完成，分析时间大大缩短，为患者争取宝贵的检测分析时间；结果呈现更加直观，可直接用于指导实际临床工作。
附图说明
图1显示了验证本发明基因panel的OR_GRS单因素逻辑回归分析模型的训练集验证结果的受试者工作特征曲线(receiver operating characteristic，ROC)。纵坐标为真阳性率(True positive rate，TPR)或者灵敏度(Sensitivity)，横坐标为假阳性率(Falsepositive rate，FPR)或者特异性(Specificity)。
图2显示了验证本发明基因panel的OR_GR单因素逻辑回归分析模型的训练集分层5折交叉验证结果的ROC曲线。
图3显示了验证本发明基因panel的EV_GRS单因素逻辑回归分析模型的训练集验证结果的ROC曲线。
图4显示了验证本发明基因panel的EV_GRS单因素逻辑回归分析模型的训练集分层5折交叉验证结果的ROC曲线。
图5显示了验证本发明基因panel的OR_GRS多因素逻辑回归分析模型的训练集验证结果的ROC曲线。
图6显示了验证本发明基因panel的OR_GRS多因素逻辑回归分析模型的训练集分层5折交叉验证结果的ROC曲线。
图7显示了验证本发明基因panel的EV_GRS多因素逻辑回归分析模型的训练集验证结果的ROC曲线。
图8显示了验证本发明基因panel的EV_GRS多因素逻辑回归分析模型的训练集分层5折交叉验证结果的ROC曲线。
图9显示了验证本发明基因panel的OR_GRS单因素逻辑回归分析模型对测试集预测结果的ROC曲线。
图10显示了验证本发明基因panel的EV_GRS单因素逻辑回归分析模型对测试集预测结果的ROC曲线。
图11显示了验证本发明基因panel的OR_GRS多因素逻辑回归分析模型对测试集预测结果的ROC曲线。
图12显示了验证本发明基因panel的EV_GRS多因素逻辑回归分析模型对测试集预测结果的ROC曲线。
具体实施方式
以下结合具体实施例对本发明做进一步详细说明。应理解，以下实施例仅用于说明本发明而非用于限定本发明的范围。
本文中的基因panel又可称为基因集(gene set)，是所有与激素性股骨头坏死(GA-ONFH)易感性相关的基因集合或基因组合。
基因(检测)panel是高通量基因检测和基因测序发展起来后用的一个词语，它是指在检测中将若干基因对应的探针设计到同一张捕获芯片上以捕获目标DNA并用于后续的基因测序。在检测中不只是检测一个位点、一个基因，而是同时检测多个位点、多个基因、多个位点，这些位点和基因需要按照一个标准进行选择和组合，需要一个检测panel。
本发明应用基因panel进行GA-ONFH易感性相关基因检测的手段主要是多重PCR靶向测序装置和配套的试剂盒。
在优选的实施方式中，该试剂盒除了各种引物外，还可分别包括下述物品中的至少之一：携带工具，其空间划分为可以收容一种或多种容器、96孔板或板条的限定空间，该容器例如是试剂盒、药瓶、试管、和类似物，每样容器都含有一个单独的用于本发明方法的组分；说明书，其可以写在瓶子、试管和类似物上，或者写在一张单独的纸上，或者在容器的外部或内部，例如是带有操作演示视频APP下载窗口比如二维码的纸件，说明书也可以是多媒体的形式，比如CD、U盘、网盘等。
筛选构建GA-ONFH预测基因panel和多重PCR引物设计的方法包括如下步骤：
1、从已公开文献资源中选择已公开骨坏死及糖皮质激素代谢相关基因，所述已公开骨坏死及糖皮质激素代谢相关基因包括表1中所列人类基因位点。
2、将上述骨坏死及糖皮质激素代谢相关基因合并，去冗余，并通过NCBI officename、HGNC approved Official Symbol系统确定标准基因名，获得GA-ONFH预测靶向测序基因集，如下述表1所示。
表1、多态性位点基因集列表
3、多重PCR引物设计：根据步骤2中获得的GA-ONFH预测靶向测序基因集，从人类基因组中寻找可以设计多重PCR引物的设计区域，设计并生成多重PCR引物；所述引物经过合理的设计，能覆盖基因集多态性位点，用于单管多重PCR扩增时特异性良好，引物彼此之间不引发引物二聚体，且均一性较好。结果如表2所示。
表2、用于检测基因panel中人类基因组位点的引物序列
4、靶向测序结果的生物信息学分析：将下机得到的.bcl文件，使用数据拆分软件bcl2fastq(v2.20.0.422)，依据各个样本的Sample Index拆分成各样本的原始的.fastq文件，即raw data；使用Trimmomatic(v0.36)去除raw data中的接头序列、低质量碱基等，生成clean data.；使用BWA(v0.7.17-r1188)，将clean data比对到hg19参考基因组，产生.sam文件；使用Samtools(v1.7)，将.sam文件进行排序并转换成.sorted.bam文件；使用VarScan(v2.4.3)，以.sorted.bam为输入，进行SNP和Indel变异检测，并产生.vcf文件；从.vcf文件中提取相关信息，最终生成各位点的基因型信息。
5、建立基于多态性位点的GA-ONFH风险预测模型：
1)以OR值作为权重的遗传风险评分(an odds ratio weighted genetic riskscore，OR_GRS)，计算所有纳入模型SNP的OR值权重，该评分方法的计算公式如下：
ωi＝ln(ORi)，
a、OR_GRS单因素逻辑回归分析模型训练，公式为：
logit P(D＝1|G)＝α+β(OR_GRS)，参数值α＝0.64[-0.22，1.49]，β＝0.35[0.20，0.51]。该模型内部验证训练集得到的AUC为0.93(p-value＝4.32e-11)，测试集预测得到的AUC值为0.95(p-value＝1.15e-11)。
b、同时考虑OR_GRS、性别(Sex)、年龄(Age)、原发病(PrimaryDisease)进行多因素逻辑回归分析模型训练，公式如下：
logit P(D＝1|G)
＝α+β1(OR_GRS)+β2(Sex_0)+β3(Sex_1)+β4(Age_0)+β5(Age_1)+β6(PrimaryDisease_1)+β7(PrimaryDisease_2)，
参数值分别为：α＝0.11[-0.63，0.86]，β1＝0.54[0.22，0.85]，β2＝0.98[-0.272.23]，β3＝-0.86[-2.40，0.67]，β4＝＝1.15[-0.07，2.37]，β5＝-1.04[-2.37，0.30]，β6＝-1.61[-4.25，1.03]，β7＝-2.63[-0.04，5.30]。该模型内部验证训练集得到的AUC为0.97(p-value＝1.72e-12)，测试集预测得到的AUC值为0.97(p-value＝3.19e-13)。
2)可释方差遗传风险评分(plained variance weighted genetic risk score，EV_GRS)，同时考虑SNP效应和风险等位基因频率(即最小等位基因频率MAF)，该评分方法的计算公式如下：
a、EV_GRS单因素逻辑回归分析模型训练，回归公式为：
logit P(D＝1|G)＝α+β(EV_GRS)，
参数值为：α＝0.90[-0.02，1.82]，β＝0.57[0.31，0.82]。该模型进行内部验证所得到的AUC为0.93(p-value＝4.92e-11)，测试集预测得到的AUC值为0.95(p-value＝1.60e-11)。
b、同时考虑GRS、性别(Sex)、年龄(Age)、原发病(PrimaryDisease)四个因素进行多因素逻辑回归分析模型训练，公式为：
logit P(D＝1|G)＝α+β1(EV_GRS)+β2(Sex_0)+β3(Sex_1)+β4(Age_0)+β5(Age_1)+β6(PrimaryDisease_1)+β7(PrimaryDisease_2)，
参数值分别为：α＝0.35[-0.40，1.10]，β1＝0.81[0.36，1.26]，β2＝1.02[-0.22，2.26]，β3＝-0.67[-2.12，0.79]，β4＝1.19[-0.03，2.41]，β5＝-0.84[-2.09，0.41]，β6＝-1.85[-4.55，0.85]，β7＝2.44[-0.07，4.94]。该模型进行内部验证所得AUC为0.96(p-value＝1.84e-12)，测试集预测得到的AUC值为0.97(p-value＝3.19e-13)。
上述的AUC值(曲线下面积)高达0.93-0.97，证明本发明筛选构建的基因panel用于预测GA-ONFH具有高度的可靠性和准确性。
本领域技术人员容易理解，上述GA-ONFH风险预测模型很容易通过编程、或者通过数学软件包形式被输入到计算机、基因检测装置比如illumina测序仪、云服务器的信息处理模块。根据受试者的基因检测结果，能够方便地预测出受试者的激素性股骨头坏死易感性，为医生给药方案选择提供参考。
如下以GA-ONFH易感性基因panel的筛选和验证实施例说明本发明的技术效果。实施例中涉及到的百分含量，除特别说明外(比如明示为体积百分比率或比例)，皆指质量百分含量。
实施例1多态性位点检测
仪器：PCR仪、Qubit荧光定量仪、安捷伦4200片段分析仪、illumina高通量测序仪。
按下述步骤检测多态性位点：
1、样本DNA提取：全血样本，采用QIAamp DNA Blood Mini Kit核酸提取试剂盒，参考试剂盒说明书进行操作，提取样本中的DNA，并用Quibt定量，-20℃冻存，使用前室温解冻。
2、DNA文库构建：
1)第一轮多重PCR扩增
a)按照表制备多重PCR反应液，DNA的起始量为40ng；混匀并短暂离心，将反应混合液收集至PCR管底部。
b)将PCR管放入带有加热盖的热循环器中，并使用下列程序执行多重PCR扩增反应：
2)第一轮磁珠纯化
a)向30μl PCR产物加入27μl室温平衡后的AMPure XP磁珠，用移液器轻缓吸打混匀20次；
b)室温孵育5min后，将PCR管置于磁力架上静置5min；
c)彻底移除上清，将PCR管从磁力架取下，向管内加入50μl YF buffer B用移液器轻缓吸打混匀20次；
d)室温孵育5min后，将PCR管置于磁力架上静置5min；
e)移除上清，PCR管继续放置在磁力架上，向管内加入200μl 80％乙醇溶液，静置30s；
f)移除上清，PCR管继续放置在磁力架上，向管内加入200μl 80％乙醇溶液，静置30s后彻底移除上清(使用10μl移液器移除底部残留乙醇溶液)；
g)室温静置3-5min，使残留乙醇彻底挥发；
h)将PCR管从磁力架取下，加入24μl Nuclease-free water，用移液器轻缓吸打混匀20次，重悬磁珠，避免产生气泡，室温静置3min；
i)将PCR管重新置于磁力架上，静置3min；
j)用移液器吸取20μl上清液，转移到新的200μl PCR管内，管内上清液为多重PCR产物。
3)第二轮接头序列PCR反应
a)按照表制备PCR反应液，混匀并短暂离心，将反应混合液收集至PCR管底部。
b)将PCR管放入带有加热盖的热循环器中，并使用下列程序执行多重PCR扩增反应
4)第二轮磁珠纯化
a)向30μl PCR产物加入27μl室温平衡后的AMPure XP磁珠，用移液器轻缓吸打混匀20次；
b)室温孵育5min后，将PCR管置于磁力架上静置5min；
c)彻底移除上清，将PCR管从磁力架取下，向管内加入50μl YF buffer B用移液器轻缓吸打混匀20次；
d)室温孵育5min后，将PCR管置于磁力架上静置5min；
e)移除上清，PCR管继续放置在磁力架上，向管内加入200μl 80％乙醇溶液，静置30s；
f)移除上清，PCR管继续放置在磁力架上，向管内加入200μl 80％乙醇溶液，静置30s后彻底移除上清(使用10μl移液器移除底部残留乙醇溶液)；
g)室温静置3-5min，使残留乙醇彻底挥发；
h)将PCR管从磁力架取下，加入24μl Nuclease-free water，用移液器轻缓吸打混匀20次，重悬磁珠，避免产生气泡，室温静置3min；
i)将PCR管重新置于磁力架上，静置3min；
j)用移液器吸取20μl上清液，转移到新的200μl PCR管内，管内上清液为多重PCR测序文库。
5)文库定量及片段分析
a)取1μl文库使用
3.0Fluorometer(Qubit dsDNA HS Assay Kit)进行文库浓度测定，记录文库浓度。
b)取1μl文库样本使用Agilent 4200 Bioanalyzer system(High SensitivityDNA Kit)进行文库片段长度和纯度测量，正常文库的靶片段分布区间在300bp–450bp之间，主峰在409bp左右。
3、上机测序：将建好的文库，准确定量并稀释到合适浓度，参考illumina相应操作说明书，将每个样本的DNA扩增文库按照500M数据量进行上机测序。
4、生物信息学分析
1)数据拆分：将下机得到的.bcl文件，使用数据拆分软件bcl2fastq(v2.20.0.422)，依据各个样本的Sample Index拆分成各样本的原始的.fastq文件,即rawdata。
2)数据清洗：使用Trimmomatic(v0.36)去除raw data中的接头序列、低质量碱基等，生成cleandata。
3)序列比对：使用BWA(v0.7.17-r1188)，将clean data比对到hg19参考基因组，产生.sam文件。
4)比对结果排序：使用Samtools(v 1.7)，将.sam文件进行排序并转换成.sorted.bam文件。
5)多态性位点检测：使用VarScan(v2.4.3)，以.sorted.bam为输入，进行SNP和Indel变异检测，并产生.vcf文件。从.vcf文件中提取相关位点信息，最终生成各位点的基因型信息。
实施例2遗传风险评分的计算及模型训练数据准备
1、按照实施例1的方法，对162例回顾性样本进行Panel检测，获得样本的多态性位点基因型信息。
2、OR(odd ratio)值计算：一个SNP位点有两个等位基因，记为Allele1、Allele2。假设Allele1为风险等位基因，那么按照下面的公式计算风险等位基因的OR值：
等位基因1(Allele1)的OR值：Odds Ratio＝(A/B)/(C/D)＝AD/BC
3、风险等位基因人群频率收集：考虑到各数据库中的中国人群量较小，从ensembl数据库(http：//grch37.ensembl.org/index.html)上收集风险等位基因在亚种人群中的人群频率。以千人基因组计划为主，在千人基因组数据库中搜索不到的位点，从gnomadAD数据库中收集。
4、SNP位点间的连锁不平衡分析：使用R包LDlinkR对所有位点在亚洲人群中进行连锁不平衡分析，互相存在连锁不平衡的位点只保留其中的一个。
5、计算每个样本的遗传风险分值GRS(genetic risk score)：我们以G表示所有SNP位点风险等位基因的集合向量(Gi表示第i个SNP位点的风险等位基因的数量)。对于野生基因型，因不含有风险等位基因，故Gi＝0；对于杂合突变基因型，含有1个风险等位基因，故Gi＝1；对于纯合突变基因型，含有2个风险等位基因，故Gi＝2。我们采用了两种风险评分方法计算GRS，以评估不同评分方法对坏死风险预测模型的影响。
1)以OR值作为权重的遗传风险评分(an odds ratio weighted genetic riskscore，OR GRS)：考虑SNP对坏死的不同效应，以SNP效应作为权重，计算所有纳入模型SNP的OR值权重，该评分方法的计算公式如下：
ωi＝ln(ORi)
2)可释方差遗传风险评分(plained variance weighted genetic risk score，EV_GRS)，同时考虑SNP效应和风险等位基因频率(即最小等位基因频率MAF)，该评分方法的计算公式如下：
6、分层抽样：考虑到随机抽样可能会造成测试集和预测集样本种类上的偏倚，进而影响最终的训练及预测效果，因此采用分层抽样。
1)样本标记：对样本按照疾病风险、性别、年龄区间、是否为坏死组进行4个字符的标记。其中疾病风险3种，分别为1、2、3；性别2种，女性标记为0，男性标记为1；年龄区间2种，<40岁标记为0，>＝40岁标记为1；是否坏死涉及2种情形，对照标记为0，坏死标记为1。162个样本最终产生22种标记，其中12种为对照组的，另外10种为坏死组的。
2)样本分层：按照22种标记对162个患者的样本进行分层，主要考虑疾病、兼顾性别和年龄因素，并尽可能保证测试集和预测集的三种因素涉及患者的比例大体一致。
3)抽取训练集：按照各层在训练集和预测集比例，在各层内部随机抽取训练集，其余样本做测试集。
7、产生单因素模型和多因素模型测试、训练数据矩阵：对于单因素模型，仅考虑GRS一个因素，分别在OR_GRS风险评分和EV_GRS风险评分下的构建训练集、测试集矩阵。对于多因素模型，则同时考虑GRS因素、性别因素、年龄因素、原发病因素，在OR_GRS风险评分和EV_GRS风险评分下的构建训练集、测试集矩阵时加入年龄因素、性别因素、原发病因素的数据。此时，由于性别、年龄、原发病属于分类变量，因此这些变量需要先转化成哑变量。性别涉及2个哑变量，即Sex_0、Sex_1；年龄涉及2个哑变量，即Age_0、Age_1；原发病涉及3个哑变量，分别代表已知低坏死风险原发病(PrimaryDisease_1)、未知坏死风险原发病(PrimaryDisease_2)和高坏死风险原发病(PrimaryDisease_3)，原发病的风险划分以临床经验为依据，低风险疾病主要包括原发性血管炎、腹膜后纤维化、白塞氏病、干燥综合征和急性肾衰竭；高风险疾病主要包括系统性红斑狼疮和成人斯蒂尔病，其他疾病列入未知坏死风险原发病。为避免过拟合，只使用其中2个哑变量进行分析，即PrimaryDisease_1、PrimaryDisease_2。
实施例3基于OR_GRS风险评分的单因素逻辑回归分析模型的训练及内部验证
1、基于OR_GRS风险评分的单因素逻辑回归分析模型训练：仅考虑GRS一个因素，在OR_GRS风险评分下对所有训练集样本进行单因素逻辑回归分析模型训练。回归公式如下：
logit P(D＝1|G)＝α+β(OR_GRS)。
2、OR_GRS风险评分训练得到的单因素回归模型的参数值分别为α＝0.64[-0.22，1.49]，β＝0.35[0.20，0.51]。
3、采用训练集对该模型进行内部验证，AUC为0.93(p-value＝4.32e-11)，结果如图1所示。
4、采用分层5折交叉验证该模型，AUC为0.94±0.03，结果如图2所示。
5、采用自助法对基于OR GRS风险评分的单因素逻辑回归分析模型进行内部验证：对训练集进行1000次的有放回的随机抽样，产生1000个测试集，AUC为0.93±0.03。
实施例4基于EV_GRS风险评分的单因素逻辑回归分析模型的训练及内部验证
1、基于EV_GRS风险评分的单因素逻辑回归分析模型训练：仅考虑GRS一个因素，在EV_GRS风险评分下对所有训练集样本进行单因素逻辑回归分析模型训练。回归公式如下：
logit P(D＝1|G)＝α+β(EV_GRS)。
2、EV_GRS风险评分训练得到的单因素回归模型的参数值分别为α＝0.90[-0.02，1.82]，β＝0.57[0.31，0.82]。
3、采用训练集对该模型进行内部验证，AUC为0.93(p-value＝4.92e-11)，结果如图3所示。
4、采用分层5折交叉验证该模型，AUC为0.95±0.05，结果如图4所示。
5、采用自助法对基于EV_GRS风险评分的单因素逻辑回归分析模型进行内部验证：对训练集进行1000次的有放回的随机抽样，产生1000个测试集，AUC为0.93±0.03。
实施例5基于OR_GRS风险评分的多因素逻辑回归分析模型的训练及内部验证
1、多因素逻辑回归分析模型训练及模型预测：同时考虑GRS、性别、年龄、原发病四个因素，在OR_GRS风险评分下对所有训练集样本进行多因素逻辑回归分析模型训练。回归公式如下：
logit P(D＝1|G)
＝α+β1(OR_GRS)+β2(Sex_0)+β3(Sex_1)+β4(Age_0)+β5(Age_1)+β6(PrimaryDisease_1)+β7(PrimaryDisease_2)
2、OR_GRS风险评分训练得到的多因素回归模型的参数值分别为α＝0.11[-0.63，0.86]，β1＝0.54[0.22，0.85]，β2＝0.98[-0.272.23]，β3＝-0.86[-2.40，0.67]，β4＝1.15[-0.07，2.37]，β5＝-1.04[-2.37，0.30]，β6＝-1.61[-4.25，1.03]，β7＝-2.63[-0.04，5.30]。
3、采用训练集对该模型进行内部验证，AUC为0.97(p-value＝1.72e-12)，结果如图5所示。
4、采用分层5折交叉验证该模型，AUC为0.96±0.02，结果如图6所示。
5、采用自助法对基于OR_GRS风险评分的多因素逻辑回归分析模型进行内部验证：对训练集进行1000次的有放回的随机抽样，产生1000个测试集，AUC为0.96±0.02。
实施例6基于EV_GRS风险评分的多因素逻辑回归分析模型的训练及内部验证
1、多因素逻辑回归分析模型训练及模型预测：同时考虑GRS、性别、年龄、原发病四个因素，在EV_GRS风险评分下对所有训练集样本进行多因素逻辑回归分析模型训练。回归公式如下：
logit P(D＝1|G)
＝α+β1(EV_GRS)+β2(Sex_0)+β3(Sex_1)+β4(Age_0)+β5(Age_1)+β6(PrimaryDisease_1)+β7(PrimaryDisease_2)
2、EV_GRS风险评分训练得到的多因素回归模型的参数值分别为α＝0.35[-0.40，1.10]，β1＝0.81[0.36，1.26]，β2＝1.02[-0.22，2.26]，β3＝-0.67[-2.12，0.79]，β4＝1.19[-0.03，2.41]，β5＝-0.84[-2.09，0.41]，β6＝-1.85[-4.55，0.85]，β7＝2.44[-0.07，4.94]。
3、采用训练集对该模型进行内部验证，AUC为0.96(p-value＝1.84e-12)，结果如图7所示。
4、采用分层5折交叉验证该模型，AUC为0.97±0.03，结果如图8所示。
5、采用自助法对基于EV_GRS风险评分的多因素逻辑回归分析模型进行内部验证：对测试集进行1000次的有放回的随机抽样，产生1000个测试集，AUC为0.96±0.02。
实施例7基于OR_GRS风险评分的单因素逻辑回归分析模型的预测
使用测试集对基于OR_GRS风险评分的单因素逻辑回归分析模型进行测试，该模型对测试集预测得到的AUC值为0.95(p-value＝1.15e-11)，结果如图9所示。
实施例8基于EV_GRS风险评分的单因素逻辑回归分析模型的预测
使用测试集对基于EV_GRS风险评分的单因素逻辑回归分析模型进行测试，该模型对测试集预测得到的AUC值为0.95(p-value＝1.60e-11)，结果如图10所示。
实施例9基于OR_GRS风险评分的多因素逻辑回归分析模型的预测
使用测试集对基于OR_GRS风险评分的多因素逻辑回归分析模型进行测试，该模型对测试集预测得到的AUC值为0.97(p-value＝3.19e-13)，结果如图11所示。
实施例10基于EV_GRS风险评分的多因素逻辑回归分析模型的预测
使用测试集对基于EV_GRS风险评分的多因素逻辑回归分析模型进行测试，该模型对测试集预测得到的AUC值为0.97(p-value＝3.19e-13)，结果如图12所示。
上述各实施例的AUC值为0.93-0.97，表明了本发明的基因panel在预测GA-ONFH易感性中具有的高度可靠性和准确率。
以上所述仅为本发明的较佳实施例，并非对本发明任何形式上和实质上的限制，应当指出，对于本技术领域的普通技术人员，在不脱离本发明方法的前提下，还将可以做出若干改进和补充，这些改进和补充也应该视为本发明的保护范围。凡熟悉本专业的技术人员，在不脱离本发明的精神和范围的情况下，当可利用以上所揭示的技术内容而做出的些许更动、修改与演变的等同变化，均为本发明的等效实施例；同时，凡依据本发明的实质技术对上述实施例所作的任何等同变化的更动、修饰与演变，均仍属于本发明的技术方案的范围内。
SEQUENCE LISTING
<110> 复旦大学附属中山医院
骏实生物科技（上海）有限公司
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<213> 人工序列(Artificial Sequence)
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<213> 人工序列(Artificial Sequence)
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<212> DNA
<213> 人工序列(Artificial Sequence)
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<212> DNA
<213> 人工序列(Artificial Sequence)
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<212> DNA
<213> 人工序列(Artificial Sequence)
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<212> DNA
<213> 人工序列(Artificial Sequence)
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<210> 80
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 80
ttctcacaag tgaatttagg caaactaaa 29
<210> 81
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 81
tcaccatgtc ctatgatatg taatataaaa gaatg 35
<210> 82
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 82
aaataatgaa agtgtcggat gataaagca 29
<210> 83
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 83
tccataagaa tctgtagttg ggctg 25
<210> 84
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 84
tgggaaaact gattaaatag aaataataga cgaa 34
<210> 85
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 85
ctgggagtta gaatctgagt tctgg 25
<210> 86
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 86
ccatgcgggg tccaacc 17
<210> 87
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 87
agagcctgtg ggtatccctc 20
<210> 88
<211> 16
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 88
tctccagggg cggctc 16
<210> 89
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 89
aggtgccttt ggtgaaagta atct 24
<210> 90
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 90
tgcttttaaa aggttgctca ggtg 24
<210> 91
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 91
agtcctcatt ctctctgtag ggg 23
<210> 92
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 92
cagcaggcca tccagacag 19
<210> 93
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 93
tgtcttctct gaactaagtg acatgtt 27
<210> 94
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 94
aagtgcacac agaaggcttc taa 23
<210> 95
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 95
gtcccaagaa catgtgttta cttcttt 27
<210> 96
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 96
ccaccagtcg tcggaagc 18
<210> 97
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 97
atgagcccct ctctgtccc 19
<210> 98
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 98
aatgattagc aggagtcaga cacc 24
<210> 99
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 99
ggggatcccg ctgacct 17
<210> 100
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 100
gctcagactc cagcatcacc 20
<210> 101
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 101
ctgaggcctg ttccagcag 19
<210> 102
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 102
ggcaccagct ggctctg 17
<210> 103
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 103
ctgccgtccc aatgtgacc 19
<210> 104
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 104
ggttccttga ggacagtcac c 21
<210> 105
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 105
ttcagcatct cccactatgt tgg 23
<210> 106
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 106
tagagtagtt gtttgaggca cacaa 25
<210> 107
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 107
ggcttttctg gaatagagtg tcctat 26
<210> 108
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 108
ataggggact tctccggtga g 21
<210> 109
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 109
ggcttttctg gaatagagtg tcctat 26
<210> 110
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 110
ataggggact tctccggtga g 21
<210> 111
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 111
gccgtttgcc aagctcatg 19
<210> 112
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 112
atacataaat aagtcactga taaaactgga ttgt 34
<210> 113
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 113
attaccaatg gattcttaaa atcttcagga g 31
<210> 114
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 114
tcatcactgc cccttcaata gc 22
<210> 115
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 115
tctcttgtag gataagacct ctctcc 26
<210> 116
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 116
aaagctataa gaaatgccat tacttgctaa 30
<210> 117
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 117
tctccattga gatgactcct acga 24
<210> 118
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 118
tttcttcttc tcttccttgg gcaa 24
<210> 119
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 119
tctccattga gatgactcct acga 24
<210> 120
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 120
tttcttcttc tcttccttgg gcaa 24
<210> 121
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 121
gaagagaaga agaaacgaga gggaa 25
<210> 122
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 122
tgagcatctc agagtcacaa gtg 23
<210> 123
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 123
gaagagaaga agaaacgaga gggaa 25
<210> 124
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 124
tgagcatctc agagtcacaa gtg 23
<210> 125
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 125
gctgtccaga aggtaagatg atctt 25
<210> 126
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 126
tctgaaaagc ttgtactcag ttcca 25
<210> 127
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 127
ccccctcctc ttcccgg 17
<210> 128
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 128
cgctccgggt agagggg 17
<210> 129
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 129
ccccctcctc ttcccgg 17
<210> 130
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 130
cgctccgggt agagggg 17
<210> 131
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 131
cactgaacag ggagacgctc 20
<210> 132
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 132
acacacatac acacacacag tca 23
<210> 133
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 133
tttactggcg gaaatgaaat ggaag 25
<210> 134
<211> 32
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 134
aaactgaatt taaaaacatt ttattgggac ca 32
<210> 135
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 135
attctccttg tgctttagga ccctg 25
<210> 136
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 136
ctcctggcat tggatattgt tctaacaa 28
<210> 137
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 137
agtggggatt ttactgatag tgacag 26
<210> 138
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 138
gcagaggaaa agaaagaaat ctggg 25
<210> 139
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 139
caggggtggg acgttgtc 18
<210> 140
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 140
tcctcagaaa caatcggccc 20
<210> 141
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 141
gacctctcag gaaaaagtgc tagat 25
<210> 142
<211> 32
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 142
ttattaagca atttcagata aaggcagttt tc 32
<210> 143
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 143
cagatgaatt tttgcaatct agaaaaggac 30
<210> 144
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 144
ctttcagctt ccgcagtttc g 21
<210> 145
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 145
gcacttattt gcatgtcacg ct 22
<210> 146
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 146
agagaggtga gatcaggggc 20
<210> 147
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 147
atgcaacagc ccccgtg 17
<210> 148
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 148
gtggctggct gatttctgga 20
<210> 149
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 149
ggctcacctt cctgcgg 17
<210> 150
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 150
gaagcaatag actggaaccc tgta 24
<210> 151
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 151
cactcagggg tgtcagagga 20
<210> 152
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 152
ggtggggaag gatatcgaca aag 23
<210> 153
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 153
cactcagggg tgtcagagga 20
<210> 154
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 154
ggtggggaag gatatcgaca aag 23
<210> 155
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 155
accgagactg tcccatgct 19
<210> 156
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 156
tcgaagaagg cggatccga 19
<210> 157
<211> 16
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 157
ccccgtcccc tgtcca 16
<210> 158
<211> 15
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 158
ggtcgggaag ggcgc 15
<210> 159
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 159
ggagttcata taacaggcac ttttgtag 28
<210> 160
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 160
acagtaggtg ctcaataaat atttgctg 28
<210> 161
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 161
caaggaccca cggctagaac 20
<210> 162
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 162
atcccctttt gtgaaaacaa cacc 24
<210> 163
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 163
aaaccacatc tctgtttcct tgaga 25
<210> 164
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 164
gtcagctgta gaaaggagca tagtta 26
<210> 165
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 165
ctgcctctta ccaagcataa ttactttt 28
<210> 166
<211> 32
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 166
aaaaatttaa tgaccacaac caagacaatt ta 32
<210> 167
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 167
atggtggctt tgtgttaaga aagttt 26
<210> 168
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 168
cagttctcta tatgcagaat aggttaaagt g 31
<210> 169
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 169
aggcttgtat ggtaatgaca aagaattac 29
<210> 170
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 170
agaaaaagaa gaatggtcat caaaagaca 29
<210> 171
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 171
gcatcaacta tgggcccttc a 21
<210> 172
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 172
ggcatacaac aggcagctac t 21
<210> 173
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 173
tctgtcattc gcaactctac tgtag 25
<210> 174
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 174
agaccagaag taaagaggta gataaattga t 31
<210> 175
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 175
accctgaagt agaataatac cagaaatttt aag 33
<210> 176
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 176
atcttggtga ccatgtaatc cagg 24
<210> 177
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 177
tctgctagaa taccactgta gagcta 26
<210> 178
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 178
ttttaaacgg gagcccagtg aa 22
<210> 179
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 179
acaaccaggg tgtgaagttc c 21
<210> 180
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 180
tctttcctat ttaaagcttt gtgtctttga 30
<210> 181
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 181
acaaccaggg tgtgaagttc c 21
<210> 182
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 182
tctttcctat ttaaagcttt gtgtctttga 30
<210> 183
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 183
gggaacgact tggcccg 17
<210> 184
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 184
gttttccaca gaccccaggt t 21
<210> 185
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 185
tttaagacac aatgattaag ctaatagggc 30
<210> 186
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 186
tgctctcaaa tgcctatgga cc 22
<210> 187
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 187
tagaaagtga ggttctgggt ttgg 24
<210> 188
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 188
gagggaggga gagagaaatg ga 22
<210> 189
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 189
ggcgcggtta tctgaagct 19
<210> 190
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 190
aaaattggag tgtattttgg ggtcttc 27
<210> 191
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 191
cctcttggtt atttgttggt tctatgg 27
<210> 192
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 192
tctatcgtct tcccgtggag aa 22
<210> 193
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 193
atcagtaagt acagatatgt caggtgatac 30
<210> 194
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 194
tttgtatgtc tgggcatcta aaacttg 27
<210> 195
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 195
taccattagt tttctttcag catgcc 26
<210> 196
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 196
tggacattgt ttttctttac agtgtttaaa g 31
<210> 197
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 197
tttagagaaa aacaatatta ctggagtagc taag 34
<210> 198
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 198
tatactttga aggaagcaga attgaagatg 30
<210> 199
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 199
gccactattt atcaagaaag acagacg 27
<210> 200
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 200
ctcttttgtt ttctgtgttt gatgctg 27
<210> 201
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 201
cccttgctcc ctgaacgtc 19
<210> 202
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 202
ggtattgact gcgaacctct actt 24
<210> 203
<211> 32
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 203
gatgactttt aaaacatcca aaattatcac ga 32
<210> 204
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 204
gctcagttgt ttctgatggt catg 24
<210> 205
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 205
caatctcttc ttcactctgg agatctt 27
<210> 206
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 206
ctggtcttga actcctggct c 21
<210> 207
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 207
aaccaactga aaatcgggtc aataatag 28
<210> 208
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 208
aaaaagcttt gtaaattttt cttttaacag tcg 33
<210> 209
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 209
tttagtaata aacactttaa tccttgtgtg gtata 35
<210> 210
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 210
ttggaaactt ctttagttgg gacct 25
<210> 211
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 211
tcactttctg gaaataaaga gcatatttga t 31
<210> 212
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 212
gagcttggtt tgtttgaaga taaggat 27
<210> 213
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 213
tcactttctg gaaataaaga gcatatttga t 31
<210> 214
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 214
gagcttggtt tgtttgaaga taaggat 27
<210> 215
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 215
cagcctgacc ccaagcg 17
<210> 216
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 216
cattccaccg aaatctcaaa ggtg 24
<210> 217
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 217
atggtggtat tccaatgaga taaattcatt t 31
<210> 218
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 218
ttaatgctta cagttacatg ttttaaaaag tcc 33
<210> 219
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 219
aacgcgcagg tgctctc 17
<210> 220
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 220
gtcagtgcgt acctctgtgg 20
<210> 221
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 221
actggcttat gggtaaagca ttagat 26
<210> 222
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 222
ggatggctcc ctcagttgc 19
<210> 223
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 223
accatttgtc ccttgtgctg t 21
<210> 224
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 224
atctgatggc accttcttga acata 25
<210> 225
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 225
gcaggaaacc agaacaagtg c 21
<210> 226
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 226
agatgaggac aggggtcagg 20
<210> 227
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 227
gcaggaaacc agaacaagtg c 21
<210> 228
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 228
agatgaggac aggggtcagg 20
<210> 229
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 229
acacacacga atggacaatg aga 23
<210> 230
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 230
gctcacttac tgactcgata gcc 23
<210> 231
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 231
gccctggaga cctacggt 18
<210> 232
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 232
tgccttaaag tattgtaggc cttca 25
<210> 233
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 233
ttgaatggtt cctgcttttg tctaaataa 29
<210> 234
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 234
tgtatttgct tccagaaaag ttgacc 26
<210> 235
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 235
gttaagggat atggtctttt aggaaaacc 29
<210> 236
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 236
gactcttctt gtaggcgtct gag 23
<210> 237
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 237
taagaattaa cccgttccca tccc 24
<210> 238
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 238
ctacattcac gcattaatga gatgatgat 29
<210> 239
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 239
gtggtttggt tttgctggct t 21
<210> 240
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 240
tataggaaaa tgtgttgcat actgtaagtg 30
<210> 241
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 241
cccatttcat atcattttag ttctttgaat tcat 34
<210> 242
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 242
tcagatcact gcaaagtgtg agaa 24
<210> 243
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 243
agaaacatgt gagggatctg atgg 24
<210> 244
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 244
ttattgtaca gattaaatga ggtcttaagg ttc 33
<210> 245
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 245
agcttttatt tggttataag cttttgtatg c 31
<210> 246
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 246
gtagaagaaa cagaagttga tgcagaatat 30
<210> 247
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 247
ttttccatgt cttctgcgcc t 21
<210> 248
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 248
gcagcctgga gatcacagac 20
<210> 249
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 249
gccctccttg gtgtccttaa c 21
<210> 250
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 250
tacagccaga gagagaagaa aagattc 27
<210> 251
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 251
attcctctcc aatgtctatc aaagtcat 28
<210> 252
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 252
aatcccttgt actcagctcc aatc 24
<210> 253
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 253
ggatttcata ccatcctagg ccc 23
<210> 254
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 254
ggtggacaaa agggagatga taca 24
<210> 255
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 255
agtttactta cagaaatgaa tgtgtgtgta a 31
<210> 256
<211> 32
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 256
cagatacttt ttaccttttg aattttgttc ca 32
<210> 257
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 257
cagataagca gatatcactc agtttatagt aaag 34
<210> 258
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 258
tccttcatcg atgaataata tagttagaat ttcag 35
<210> 259
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 259
tcaacacaat ttttatgtac aaattacgaa atct 34
<210> 260
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 260
acactttcat tttcaaatct gattccga 28
<210> 261
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 261
tgagtgacga tcatctacat gtgac 25
<210> 262
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 262
acctgtaatc ccagcacttt gtt 23
<210> 263
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 263
aatgtttcct tatttcttcc gttcacc 27
<210> 264
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 264
aatgggggaa aacttgtatt ttgacc 26
<210> 265
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 265
ggcgctggaa gattttgaga ag 22
<210> 266
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 266
aggagctgaa gttctgaaat catctat 27
<210> 267
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 267
gcatcacctt catctcacga taaag 25
<210> 268
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 268
ttctttcacg aaaacaatga atgcaag 27
<210> 269
<211> 32
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 269
atatgaaata aagacagaaa atggtgatct gt 32
<210> 270
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 270
ctctatggta ctcacgatta ccttcttt 28
<210> 271
<211> 32
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 271
atatgaaata aagacagaaa atggtgatct gt 32
<210> 272
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 272
ctctatggta ctcacgatta ccttcttt 28
<210> 273
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 273
ctggccactc ttctgatcag tc 22
<210> 274
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 274
gccctcagcg tgacacc 17
<210> 275
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 275
ctagaggaaa ggagagtctt gtaagtg 27
<210> 276
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 276
cggggtgtgt ggtatctgc 19
<210> 277
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 277
tttctggagt catttctttc agtctct 27
<210> 278
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 278
gtctgccagt agaaaggggc 20
<210> 279
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 279
actggtgccg ggttttcttc 20
<210> 280
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 280
tccctttctc attcagttac ttggtg 26
<210> 281
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 281
actggtgccg ggttttcttc 20
<210> 282
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 282
tccctttctc attcagttac ttggtg 26
<210> 283
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 283
taccaagtga atgaacaatg ctaagc 26
<210> 284
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 284
ttacatcttt ggactctctg acagtc 26
<210> 285
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 285
tggaatatgg tgatcgggaa cac 23
<210> 286
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 286
cgaagatgaa agtgataaac tgagctac 28
<210> 287
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 287
tggctagtcc tgaatttata aacataggt 29
<210> 288
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 288
ctccaagacc aggaaatccg ag 22
<210> 289
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 289
ggaaaaattg agccaaatcc gagat 25
<210> 290
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 290
ccctccatgt ccactgaagc 20
<210> 291
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 291
ccttgttggg taagtccttt ctga 24
<210> 292
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 292
ggatgaattg gagtcctgtg aaaatc 26
<210> 293
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 293
atgtaggtct gaatagtgaa atatcccatt 30
<210> 294
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 294
gtaaaaagaa tactcaaata gtgaaatgat gctag 35
<210> 295
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 295
cctccccctt tatttcagga gc 22
<210> 296
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 296
caaagtgctt agctctcaga attgg 25
<210> 297
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 297
gaatcgtgcc cctttggtct at 22
<210> 298
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 298
caagtttcag ctcagagatt gcataat 27
<210> 299
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 299
gaccacattg agatggtgac ca 22
<210> 300
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 300
atgtggtctg taaactaggt cacag 25
<210> 301
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 301
ggagcctcat tcctgtctca ttt 23
<210> 302
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 302
tcagggattt aattgataga agccact 27
<210> 303
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 303
ggagcctcat tcctgtctca ttt 23
<210> 304
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 304
tcagggattt aattgataga agccact 27
<210> 305
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 305
tgcaaaggac atgatcagat tcct 24
<210> 306
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 306
gttttcttcc tctgttaggc attgtatt 28
<210> 307
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 307
atttgattat taatctccaa tatgcctcac atat 34
<210> 308
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 308
aaattaaatc ataatatgcg ctgttatctc tttc 34
<210> 309
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 309
ctctgtatac cggcccatct tg 22
<210> 310
<211> 32
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 310
tccatattag catttgcata tttttcctct at 32
<210> 311
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 311
ggacttccag attacagtaa tttaatgtga a 31
<210> 312
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 312
atttcaagat ggaatttaag ggttagatgg 30
<210> 313
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 313
gcaaagaaac agtgattgag tcagttaaaa 30
<210> 314
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 314
cgttggccct ttcttctgtc at 22
<210> 315
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 315
ccatcaggtc tttcttgaat ttggaaa 27
<210> 316
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 316
acctggttcc agcactactc t 21
<210> 317
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 317
agttttataa ttttagatct ttgctgaatt tgct 34
<210> 318
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 318
tgctaatatc ctgtggttca ctctg 25
<210> 319
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 319
ggccaaatca acactaagca gtc 23
<210> 320
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 320
tgatgaaaat gagctaaagg aattcttcat 30
<210> 321
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 321
attctaggga tcagcacttc aaaaataac 29
<210> 322
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 322
tccgaaaact aaaacaccgt ttgatt 26
<210> 323
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 323
catccattgt catttctacc ttggc 25
<210> 324
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 324
cccatccacc acttcgtcg 19
<210> 325
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 325
ctcatccaga aacgctatga caaac 25
<210> 326
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 326
ctgcatagac agttcaacag aatcttc 27
<210> 327
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 327
tctccgtgca aattctcggg 20
<210> 328
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 328
cattgatgtc attaacaaaa atcaccagg 29
<210> 329
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 329
ccaggcaata gtaaccatca ctgta 25
<210> 330
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 330
ttgctcttct ctatcaatca catttgc 27
<210> 331
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 331
caggaggcca gagaaaatcc aa 22
<210> 332
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 332
acgcgagatg aaggaaattg aaac 24
<210> 333
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 333
gggacggaga ggtgttcttt tt 22
<210> 334
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 334
cagcttataa tgaacacaca gcctg 25
<210> 335
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 335
cgttttcact tcccagttca tctaaaa 27
<210> 336
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 336
ctaagttaat gatcgctcct ttaaaaatgt tac 33
<210> 337
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 337
atccccagaa acattcaaga taatctct 28
<210> 338
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 338
aagatgggac cattccatgc c 21
<210> 339
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 339
aagatttata agtgtgtgtg caaagatgt 29
<210> 340
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 340
atctggagcg acaaatggga tac 23
<210> 341
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 341
tgctgaggag gatgggagg 19
<210> 342
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 342
ggtaactcat atctagttta aaattgtgtt tcttc 35
<210> 343
<211> 16
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 343
agaggctacg ggcgca 16
<210> 344
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 344
aagagcaatg agtaggcatc acat 24
<210> 345
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 345
cagtatttga cagaagttca atagttttgt atg 33
<210> 346
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 346
agattagcct cacggttgct g 21
<210> 347
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 347
tctaattcac tgtcatcttt gtgacttttt 30
<210> 348
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 348
cctttgcaaa tatgtgaagc ttgttatg 28
<210> 349
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 349
cagtgaaaca gactgaagaa taagtacg 28
<210> 350
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 350
tattcagcat tagagtagaa aagaaactat tgtt 34
<210> 351
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 351
atcagatcac cactacagat gttcag 26
<210> 352
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 352
ttccaccctc caatgcatga atat 24
<210> 353
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 353
tcattatatt gtatctacac tatcacctgt tagat 35
<210> 354
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 354
gcagacagtt ccatatgaca gtct 24
<210> 355
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 355
acgcttgtac cacatctcag aatt 24
<210> 356
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 356
tctgaggtcc atgtaaagaa agttagtt 28
<210> 357
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 357
ttcttctttc gcagttctgt tttgaa 26
<210> 358
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 358
atgtccttgg cacccttcat c 21
<210> 359
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 359
ccccagtatg aaccaagcga a 21
<210> 360
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 360
tcagcactgg gtaacacgaa g 21
<210> 361
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 361
cagctggcag acggaagg 18
<210> 362
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 362
tcctctgcac tccctaggc 19
<210> 363
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 363
acaccatagc tgaatgccag g 21
<210> 364
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 364
gaaaaacaat catccacagg cgtat 25
<210> 365
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 365
cagaggaagg agctgcagat g 21
<210> 366
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 366
tgggcaggga ggaaaaggta 20
<210> 367
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 367
ttctcctgcc gtattctgaa agg 23
<210> 368
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 368
atcagaagtc tgcaaataat gtgtttacat a 31
<210> 369
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 369
gtctcctatg tggcagagaa gc 22
<210> 370
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 370
acttgtccct gaggccagt 19
<210> 371
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 371
ccaaaccgcc agatatgcct 20
<210> 372
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 372
aaccttggag gaatgtaaaa gcaatg 26
<210> 373
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 373
ccaaaccgcc agatatgcct 20
<210> 374
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 374
aaccttggag gaatgtaaaa gcaatg 26
<210> 375
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 375
ccaaaccgcc agatatgcct 20
<210> 376
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 376
aaccttggag gaatgtaaaa gcaatg 26
<210> 377
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 377
ggaatgtggg gagatttgaa tttgaa 26
<210> 378
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 378
caaagggcgt tagggcgt 18
<210> 379
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 379
ggatctcaat ctccacgatg tagtc 25
<210> 380
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 380
gcatctggac atccggcc 18
<210> 381
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 381
acctgggagg ggttcctg 18
<210> 382
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 382
tcacagccag aggagaccat 20
<210> 383
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 383
agattatata aacaaagtct acagaattac acagg 35
<210> 384
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 384
agtaatctca aattgttcat tatttgttgg c 31
<210> 385
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 385
taagatgagc agctctgacc aac 23
<210> 386
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 386
atatgaacac gtggctgctg t 21
<210> 387
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 387
taagatgagc agctctgacc aac 23
<210> 388
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 388
atatgaacac gtggctgctg t 21
<210> 389
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 389
acccccaccc caacaca 17
<210> 390
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 390
taggagtggg cgcaaagtaa c 21
<210> 391
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 391
ggtgaggcca atgcacga 18
<210> 392
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 392
ccgggaaaaa tctccgttct ttc 23
<210> 393
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 393
ccttcttggt ctcttccagt cg 22
<210> 394
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 394
aaaaagaatg cctgtgtttc gaagt 25
<210> 395
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 395
ccttcttggt ctcttccagt cg 22
<210> 396
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 396
aaaaagaatg cctgtgtttc gaagt 25
<210> 397
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 397
tatttgtgca agtttactat catcaagca 29
<210> 398
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 398
aagcctatga tcctcattta acagtga 27
<210> 399
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 399
ttatagttcc cattagtcaa aactggaatt atc 33
<210> 400
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 400
ctttcttatg atccaactaa acatccagg 29
<210> 401
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 401
aggcagtgga gcaggttata ataag 25
<210> 402
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 402
tcatatctca ttctcaattc ttaattcaac agtta 35
<210> 403
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 403
ctactggcag actttcttct ctcc 24
<210> 404
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 404
atggtcatgc tgtggctgg 19
<210> 405
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 405
ccatcttcat ctccactgtc ttcat 25
<210> 406
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 406
tgtaagaact gcaaagaaat ttaattttgt ctaa 34
<210> 407
<211> 32
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 407
agtttatatt tttctcatct agccacattt ca 32
<210> 408
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 408
gacggttggc ttcatcgagt 20
<210> 409
<211> 32
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 409
agtttatatt tttctcatct agccacattt ca 32
<210> 410
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 410
gacggttggc ttcatcgagt 20
<210> 411
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 411
atgttggtag tatttcatag ctgaattatt agaaa 35
<210> 412
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 412
gaaggagatg tttttagcct catcaag 27
<210> 413
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 413
atgttggtag tatttcatag ctgaattatt agaaa 35
<210> 414
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 414
gaaggagatg tttttagcct catcaag 27
<210> 415
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 415
gcaacaattt ctttgctctc ttatgttac 29
<210> 416
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 416
atcttttata agccaaattc atttcctcca 30
<210> 417
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 417
ccacgcccag ctaattttga atttt 25
<210> 418
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 418
gagttccaac ccagcaccat 20
<210> 419
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 419
ggttagatgt tcatctctga gttcca 26
<210> 420
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 420
atttaaacaa gatttgctgc atttccg 27
<210> 421
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 421
acgaagaatc cagagtaaaa caaaaagtta 30
<210> 422
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 422
tacacggcct ataggtggca 20
<210> 423
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 423
gtaaaggcga ctggggctc 19
<210> 424
<211> 15
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 424
tgcggaacct gcgcg 15
<210> 425
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 425
gccaggccag caacaatg 18
<210> 426
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 426
aaccttccag aagtgggcag 20
<210> 427
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 427
ctgcacaaaa gagtaagtgc tgg 23
<210> 428
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 428
gttgctggcc tggctgt 17
<210> 429
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 429
ctgcacaaaa gagtaagtgc tgg 23
<210> 430
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 430
gttgctggcc tggctgt 17
<210> 431
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 431
aactaccggc aaatacccaa gaat 24
<210> 432
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 432
ctgggagtgg aaaaggctgg 20
<210> 433
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 433
aggaaccctg tggcttcct 19
<210> 434
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 434
ttttggggtg atcgttgtag ca 22
<210> 435
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 435
taaagtacgt gaatgtccag aatgga 26
<210> 436
<211> 16
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 436
gggcccacct ggttgc 16
<210> 437
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 437
cgtacagcct gggaaatata gg 22
<210> 438
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 438
tttatggctg gacagtctct g 21
<210> 439
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 439
gtgcttgggt ttttcatttg agaatg 26
<210> 440
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 440
tccatccttc cgcctgagt 19
<210> 441
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 441
ggtacaaagt gacggcaaaa tgtaa 25
<210> 442
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 442
agcaataaac accacctgaa tcatttt 27
<210> 443
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 443
taaattccca tacctgtaat attgcaactt t 31
<210> 444
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 444
tagttcttta tagaaatacg tatgatgtct ttgag 35
<210> 445
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 445
ccttaacaga ctgcaagatg ctagt 25
<210> 446
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 446
ttgggtttct aggttaaaat gctttgg 27
<210> 447
<211> 16
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 447
gaccagcacg cggtcg 16
<210> 448
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 448
ggcgctggtc ttgctgg 17
<210> 449
<211> 16
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 449
gaccagcacg cggtcg 16
<210> 450
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 450
ggcgctggtc ttgctgg 17
<210> 451
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 451
cccgttccat gtccccattt 20
<210> 452
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 452
agttatgtca tatctaagcc ccgc 24
<210> 453
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 453
cccgttccat gtccccattt 20
<210> 454
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 454
agttatgtca tatctaagcc ccgc 24
<210> 455
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 455
atttataaca tctaaatgtg tgttgttctg c 31
<210> 456
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 456
gaaagaaact gagtcccaca aagg 24
<210> 457
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 457
gtccccaacc ccagtattta ctac 24
<210> 458
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 458
ggggtggtgt ttgtgatacc a 21
<210> 459
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 459
gtccccaacc ccagtattta ctac 24
<210> 460
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 460
ggggtggtgt ttgtgatacc a 21
<210> 461
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 461
ctcccaaacc ccactcgca 19
<210> 462
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 462
gcctcacact ctccgcg 17
<210> 463
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 463
tgaagtgttg ggtgcattga ga 22
<210> 464
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 464
aaacctgtta aaagtgcaca ccg 23
<210> 465
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 465
gccactacct aatttcacac acaaaa 26
<210> 466
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 466
ttgggaagga cgtttaaaca tttcag 26
<210> 467
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 467
gtgtccacgg agtcaaggat c 21
<210> 468
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 468
catgggaaac gtggctggat a 21
<210> 469
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 469
gggaggaggt ttgaggagga 20
<210> 470
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 470
tccccttccc attcccttca 20
<210> 471
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 471
gagaagcagt tttactgatg gaactaag 28
<210> 472
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 472
ttcagtttgt agatatggag cccatt 26
<210> 473
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 473
aagcagagcc ctcttgcag 19
<210> 474
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 474
accctgcctc ccaacgta 18
<210> 475
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 475
tgagaatgga ctaatatagc ggtctaac 28
<210> 476
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 476
ctcaacaccc tgaagagaga gaatt 25
<210> 477
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 477
gaatgctatc aaggaagcta caagc 25
<210> 478
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 478
tgaagcagta ttttcgagat cttcct 26
<210> 479
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 479
ggctatcaag tctaggcgat atcatt 26
<210> 480
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 480
atctttgcta acgttatttc tttgctctat 30
<210> 481
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 481
atacaaaaag aaagcaagaa aatgatagtc ttc 33
<210> 482
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 482
cccttgttcg cgagtattac tga 23
<210> 483
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 483
atacaaaaag aaagcaagaa aatgatagtc ttc 33
<210> 484
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 484
cccttgttcg cgagtattac tga 23
<210> 485
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 485
gaacaaggga ttatgattat gccaactt 28
<210> 486
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 486
acatggctaa ttttgtcttt cttctgtt 28
<210> 487
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 487
ccatgttggc aaagcagatg g 21
<210> 488
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 488
aataggagag atgtatgcac tgtacaaa 28
<210> 489
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 489
actgggaagt aacagagaat cagc 24
<210> 490
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 490
agagcaagac ccggactcta a 21
<210> 491
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 491
cttataaatc tgtatgaacc taatgcaaca atat 34
<210> 492
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 492
gtagggcaat gttctgctgc 20
<210> 493
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 493
gcatgactat attccagttt ttctttcct 29
<210> 494
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 494
aaaaaccaac tagatcgttt tagaaagtga 30
<210> 495
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 495
tccaggagct gatgaggatg t 21
<210> 496
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 496
tgcaatggaa aatagaacgc acc 23
<210> 497
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 497
agccagacag ataatgtgtc agtc 24
<210> 498
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 498
agcctgtctt aagaatctct gttacaata 29
<210> 499
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 499
aagatgtctt ttctgccttt agttgc 26
<210> 500
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 500
tgtaaggaca gtggagttag tgaatg 26
<210> 501
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 501
ccagctggga tgtggtagtt c 21
<210> 502
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 502
ggaggtttgg ctgggctc 18
<210> 503
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 503
ccagctggga tgtggtagtt c 21
<210> 504
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 504
ggaggtttgg ctgggctc 18
<210> 505
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 505
ctttttgacc tatttctttt cactgtaata 30
<210> 506
<211> 32
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 506
aaatcctcca agatattcag tataaggtaa gt 32
<210> 507
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 507
ctcctaacac ttgcttcttc caaatc 26
<210> 508
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 508
gaaagtggac gcctagcttt ttc 23
<210> 509
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 509
ttttattttt aaacaaagat aggcagaaac aaaac 35
<210> 510
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 510
tgattcaggg aatcttctag aagctg 26
<210> 511
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 511
ctatttgcag tagaattggg acctaga 27
<210> 512
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 512
ttaaaatcac attgggaaag agaattactg t 31
<210> 513
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 513
aataaacttc tttccatatt gggccac 27
<210> 514
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 514
actctggcag gtaagtacaa tcattta 27
<210> 515
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 515
ggggtctgtg ggggagg 17
<210> 516
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 516
ggtccttgct catgatggac c 21
<210> 517
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 517
ctaccagttc tacagcgcct c 21
<210> 518
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 518
agggctctcc tcgaagttgt a 21
<210> 519
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 519
tgaggaaatt cgaggcatct gatt 24
<210> 520
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 520
gaagaggcga gtgaaggtga g 21
<210> 521
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 521
ggaaaggctt ccgaaaaaca caa 23
<210> 522
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 522
aaaacattgt tagtgaacag gtaagtcc 28
<210> 523
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 523
atgggattat ggccctaata ttttattgc 29
<210> 524
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 524
tgagtttaaa tcacttataa agcatgggg 29
<210> 525
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 525
ctcaccttgc tgagaatccc c 21
<210> 526
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 526
aagtgttcct tttggtgtga ttaatttttc 30
<210> 527
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 527
aaacatgagc ttgaaaacat tccagt 26
<210> 528
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 528
tgacagacaa cagaaagatc agagtt 26
<210> 529
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 529
tgtcttgatg ctgtaagtgt atttataaag aat 33
<210> 530
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 530
ttcaaaattg cagcttcctg ttgt 24
<210> 531
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 531
catgagaaag tgacatgagg gttaatg 27
<210> 532
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 532
ttccgacttc gcatcgcc 18
<210> 533
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 533
catgagaaag tgacatgagg gttaatg 27
<210> 534
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 534
ttccgacttc gcatcgcc 18
<210> 535
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 535
aaatcgttta aaaagtcctc ctcaagaaa 29
<210> 536
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 536
atgtttctct atatatgact atcagagcaa gtat 34
<210> 537
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 537
gctcatgtaa atgttttata aatttgaaac acag 34
<210> 538
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 538
tgtttcttta tttgtaattt ggtttttgac aatg 34
<210> 539
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 539
ttacaaaatt gcttagcata tactactaca aaaac 35
<210> 540
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 540
actaaaactg aaaggaaact aacctaatat atgg 34
<210> 541
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 541
ccacagcagc cattaaaatt attctatttt t 31
<210> 542
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 542
catttggtac tacaatacga gctatatcaa g 31
<210> 543
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 543
tttatacaac atacctcatc tccaatgga 29
<210> 544
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 544
tctgagcagt ttatccattg gttttatg 28
<210> 545
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 545
gggctcgcta aagtgtgtat ga 22
<210> 546
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 546
cctaattggt tgaatgaata gcagttgta 29
<210> 547
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 547
gtccgaagta atcctcctca tttca 25
<210> 548
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 548
agtttcattt tctaatctct tgtctaatta ggg 33
<210> 549
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 549
ccactgtgtc attaggaatt gttatcaa 28
<210> 550
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 550
caggagaact atcgctttta aggagtt 27
<210> 551
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 551
ggaaaatgaa gcatgtttag aaatcagc 28
<210> 552
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 552
ttgtttacag ccaagctcct taatttt 27
<210> 553
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 553
aagctagatg ttcagtattg caaacg 26
<210> 554
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 554
aacaggtctc aagaggacaa tctaaaa 27
<210> 555
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 555
agattgtcct cttgagacct gtttac 26
<210> 556
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 556
gaatacctgg cctccgaagc 20
<210> 557
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 557
gccatggttc agaggccc 18
<210> 558
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 558
cctagttgtg gacactggat tgatat 26
<210> 559
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 559
tccgggttgt cagggca 17
<210> 560
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 560
agaaaagcgc gaggagacg 19
<210> 561
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 561
gacgagatca gtgtggacag tc 22
<210> 562
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 562
gaaaaatgtc cggcttaact atgca 25
<210> 563
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 563
cgccggcact agtcagc 17
<210> 564
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 564
gtgtcctgga gtgcattaaa ttatttctat 30
<210> 565
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 565
ccttgggaat ttgatatcat taataccttg t 31
<210> 566
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 566
ggtttattaa ttgcatttca gaaccatcag 30
<210> 567
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 567
gaacaaatag aagaaacagc agagagg 27
<210> 568
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 568
aaacacaagt ggcttttggg c 21
<210> 569
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 569
gaacaaatag aagaaacagc agagagg 27
<210> 570
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 570
aaacacaagt ggcttttggg c 21
<210> 571
<211> 16
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 571
cgtcccgcgt gctctg 16
<210> 572
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 572
tggtgtgggt gtgtgcatc 19
<210> 573
<211> 16
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 573
gccgggcagc tcacag 16
<210> 574
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 574
gcagcctggg caaaggt 17
<210> 575
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 575
cgtcaggaag tcggccac 18
<210> 576
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 576
ctcctcctgt ctctcgctca 20
<210> 577
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 577
agggttccac ttgctttctt ctt 23
<210> 578
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 578
ctggcgctga ggtgtgg 17
<210> 579
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 579
ccccttctgc tttgcttata ctct 24
<210> 580
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 580
agcactgctg actccatgat g 21
<210> 581
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 581
tggccatctg caagccatt 19
<210> 582
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 582
cagccttacc acagccatgt 20
<210> 583
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 583
cttctcacct ctgtgccatc c 21
<210> 584
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 584
gtaatcatct aatcttcatt tctatccttg gtatt 35
<210> 585
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 585
ggacggtgga gtcatgcag 19
<210> 586
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 586
ctttccacac tgcccactgt 20
<210> 587
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 587
gtagacattg gacatcatga catgaac 27
<210> 588
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 588
caacatttat gggctcttgg tgatc 25
<210> 589
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 589
gtagacattg gacatcatga catgaac 27
<210> 590
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 590
caacatttat gggctcttgg tgatc 25
<210> 591
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 591
ggagacagta ggagtgatgc aaaa 24
<210> 592
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 592
cttgcctggt ccagatgttc tt 22
<210> 593
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 593
ggagacagta ggagtgatgc aaaa 24
<210> 594
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 594
cttgcctggt ccagatgttc tt 22
<210> 595
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 595
gaggcctgtt tgtatcccag g 21
<210> 596
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 596
gcaggaatac aggcaatcat tttca 25
<210> 597
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 597
ataagactgt agaggtctcc aaggat 26
<210> 598
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 598
gcctcctttc cacctgctc 19
<210> 599
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 599
ataagactgt agaggtctcc aaggat 26
<210> 600
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 600
gcctcctttc cacctgctc 19
<210> 601
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 601
gtatctcatt tggaactgac tgtactttc 29
<210> 602
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 602
agaatgggtt gagggttcca aaa 23
<210> 603
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 603
ctccacctgc tcctcatcct a 21
<210> 604
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 604
gggatgaggg gctctagtgt 20
<210> 605
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 605
cctgtactca catttcaaag gctattc 27
<210> 606
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 606
tcctatcctt gatcaatgag cacag 25
<210> 607
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 607
tgtcagaggg gaaatttgtt actgt 25
<210> 608
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 608
gctcttcgcc tcaggtgac 19
<210> 609
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 609
actgcctggg ggtgtgt 17
<210> 610
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 610
ttcgaatact taggagtagg ggct 24
<210> 611
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 611
aaaaacaaga cttgagcacc tgc 23
<210> 612
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 612
gaaatgagtt tgaatccagg tgctag 26
<210> 613
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 613
aaaaacaaga cttgagcacc tgc 23
<210> 614
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 614
gaaatgagtt tgaatccagg tgctag 26
<210> 615
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 615
ccactacaga gagcaaaccc tac 23
<210> 616
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 616
cctgccactc tcagcttgg 19
<210> 617
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 617
ctttcttgct gatctttctg cctg 24
<210> 618
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 618
cagactaact tggtcccatc ctg 23
<210> 619
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 619
agggtcctca acttcaggag aa 22
<210> 620
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 620
atatcccagg tgagaaatgg cac 23
<210> 621
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 621
acatggtttg tactcaacac cca 23
<210> 622
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 622
cgaagccggc agaagca 17
<210> 623
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 623
ctgatggggc tcaaggcag 19
<210> 624
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 624
ggcagaaatg tatgcaggta acac 24
<210> 625
<211> 16
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 625
ggcggcgctt caaggt 16
<210> 626
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 626
cccagtgatc tccgaccct 19
<210> 627
<211> 16
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 627
ggcggcgctt caaggt 16
<210> 628
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 628
cccagtgatc tccgaccct 19
<210> 629
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 629
ctgcggggat gagatatcat gg 22
<210> 630
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 630
gaggaaggac atgctgggc 19
<210> 631
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 631
gggaggaaac acaggtgaac tt 22
<210> 632
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 632
gttcaggctg atgaagaggg g 21
<210> 633
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 633
aagaggaaga gaaagatatg catggtta 28
<210> 634
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 634
ctagttcaca aaatacttca gagcactc 28
<210> 635
<211> 32
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 635
aggtatttta aggcagtagt ttctgtagta ta 32
<210> 636
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 636
aaaggataca tcagcatcca cgtaa 25
<210> 637
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 637
cttaggaaga gaaggtcaga gttcg 25
<210> 638
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 638
cagccggcag tgacctg 17
<210> 639
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 639
gccggccttg tacatgtagt ta 22
<210> 640
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 640
tcaaacagat agctgctaat cgtca 25
<210> 641
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 641
aaaagactaa aacaaacaga gctggg 26
<210> 642
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 642
tgtgtttctt tccctgcttt tcttc 25
<210> 643
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 643
ctttcagaca aaaagccttg gca 23
<210> 644
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 644
cagaggaggc aattgacttg ataaac 26
<210> 645
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 645
cagttacccg ctcaggtaca c 21
<210> 646
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 646
caaagatgac catcacgttg gc 22
<210> 647
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 647
cagttacccg ctcaggtaca c 21
<210> 648
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 648
caaagatgac catcacgttg gc 22
<210> 649
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 649
agaaatgtcc aagaccagcc tc 22
<210> 650
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 650
gacaggttgg tgaggaagta gtac 24
<210> 651
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 651
tgactttcca tttgccctac tgt 23
<210> 652
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 652
ggaggcacag gtctgaaagg 20
<210> 653
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 653
tgactttcca tttgccctac tgt 23
<210> 654
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 654
ggaggcacag gtctgaaagg 20
<210> 655
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 655
tgactttcca tttgccctac tgt 23
<210> 656
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 656
ggaggcacag gtctgaaagg 20
<210> 657
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 657
gtgaagaaag ctgtgttgaa actga 25
<210> 658
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 658
aagcatgttc ctcatacaca taagtcta 28
<210> 659
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 659
cggtgcccaa attgctgatg 20
<210> 660
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 660
gtacctgagc gggtaactga tg 22
<210> 661
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 661
ttgccctact gtggaccca 19
<210> 662
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 662
cacgatacag tgggaggcac 20
<210> 663
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 663
tctgtgcctc ccctctgag 19
<210> 664
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 664
cagtggctct ctgcactctg 20
<210> 665
<211> 16
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 665
tcccgaacct cccgcc 16
<210> 666
<211> 15
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 666
ggcacgtgac ccgcg 15
<210> 667
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 667
gagcaggaac aagctggca 19
<210> 668
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 668
gctgtggctc ttgtgggg 18
<210> 669
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 669
gcgttcttct ctgagcctgt t 21
<210> 670
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 670
tgagcctttg ccttcaccag 20
<210> 671
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 671
tgtccgttga ctgctatgtg g 21
<210> 672
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 672
aagagcaaat ccataccagc tgt 23
<210> 673
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 673
gtttccctct ggcccacg 18
<210> 674
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 674
aggcttgatg cttacacagt tga 23
<210> 675
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 675
gtttccctct ggcccacg 18
<210> 676
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 676
aggcttgatg cttacacagt tga 23
<210> 677
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 677
ttgtaaacct ggcacttccc c 21
<210> 678
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 678
ggactgagat gcccttgtcg 20
<210> 679
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 679
ttgtaaacct ggcacttccc c 21
<210> 680
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 680
ggactgagat gcccttgtcg 20
<210> 681
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 681
ctctcatttc atctcacctc tttttcag 28
<210> 682
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 682
tcagtagctc tctcgtccct g 21
<210> 683
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 683
ccgtcccaac ccatgcc 17
<210> 684
<211> 16
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 684
tcctctctgg ccgccc 16
<210> 685
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 685
cgccaggccg caagtta 17
<210> 686
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 686
ctgccaactc tgctctctct g 21
<210> 687
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 687
ttcagtatta ccttcctcca gtcaaatt 28
<210> 688
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 688
cctgctcctt gatgtcttta ttatatgc 28
<210> 689
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 689
ggaaaaacac agacacacac cag 23
<210> 690
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 690
taaagagcat atttacattc gttttaattg tgg 33
<210> 691
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 691
acagaaaccc agatactctg tttcatat 28
<210> 692
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 692
gacaggtgaa attgcaagca gttata 26
<210> 693
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 693
aagtgatgaa aaataagtct ggagaaattg a 31
<210> 694
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 694
cagtctccag agttggtttg ct 22
<210> 695
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 695
gaagcaacaa ttcttacttc taaaccatat aaag 34
<210> 696
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 696
attctggtag tgtttgcatt tgttctt 27
<210> 697
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 697
gaaggatagc tgaatctaat agctttgc 28
<210> 698
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 698
tttttgctgt taatctgttc tctgtgtaa 29
<210> 699
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 699
cttcatattc ttttgtccgt acaatctctt 30
<210> 700
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 700
gtctgggcag taaccggc 18
<210> 701
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 701
cttcatattc ttttgtccgt acaatctctt 30
<210> 702
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 702
gtctgggcag taaccggc 18
<210> 703
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 703
tacctcacat gccacaaaac tga 23
<210> 704
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 704
gccatttact ttctgtccat aatcatgt 28
<210> 705
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 705
atgaaataaa catggcttca taattgagga 30
<210> 706
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 706
atgaaacatt ttatatttct tactgggttt tctg 34
<210> 707
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 707
atgaaataaa catggcttca taattgagga 30
<210> 708
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 708
atgaaacatt ttatatttct tactgggttt tctg 34
<210> 709
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 709
cctcacatgc cgcaaaactg 20
<210> 710
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 710
aaactgtgac ctcctttctt ctgtta 26
<210> 711
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 711
agatcttttg tctcttgacc cactc 25
<210> 712
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 712
atttaaggtc aggatattca agcaatcac 29
<210> 713
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 713
ataacacatc ctattccaga gccac 25
<210> 714
<211> 16
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 714
gcccgcggac ctccta 16
<210> 715
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 715
ataacacatc ctattccaga gccac 25
<210> 716
<211> 16
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 716
gcccgcggac ctccta 16
<210> 717
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 717
cctgcctaga ctcccatcca 20
<210> 718
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 718
tggtgggctc ggtataccat 20
<210> 719
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 719
cctgcctaga ctcccatcca 20
<210> 720
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 720
tggtgggctc ggtataccat 20
<210> 721
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 721
tgcacgcagg agcttctg 18
<210> 722
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 722
tcgctctcca atctgtctca aatt 24
<210> 723
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 723
gatgacagag caagaccatc tctt 24
<210> 724
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 724
ttggctctca aacattacct aattgtatg 29
<210> 725
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 725
agtcttttct ccttgacttt ttactccta 29
<210> 726
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 726
aaaaattgtc attaaagcca acatttcctt 30
<210> 727
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 727
gtcggtagca ggcacagac 19
<210> 728
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 728
gacttgctag aaggtgtttc atgc 24
<210> 729
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 729
ttgcccgcta acctagattc ttaat 25
<210> 730
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 730
ctgctgagcc ggctgag 17
<210> 731
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 731
tcacaggcct agagacggg 19
<210> 732
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 732
ttttctatct tgtccagagc tcagg 25
<210> 733
<211> 16
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 733
accccatccc cggacg 16
<210> 734
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 734
ggcgagaacg tagaactggg 20
<210> 735
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 735
gagtggcacg agggaaagg 19
<210> 736
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 736
tctagagact ccagcacaga gg 22
<210> 737
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 737
agaaaagctt ctggaaagtc tgtct 25
<210> 738
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 738
tggaacaaca agtacataaa gtttgtcat 29
<210> 739
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 739
agaaaagctt ctggaaagtc tgtct 25
<210> 740
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 740
tggaacaaca agtacataaa gtttgtcat 29
<210> 741
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 741
aaaaagagac aaagtaaaag aaatgaaggt tac 33
<210> 742
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 742
cttcttcata tccagatttt tcaaacaagc 30
<210> 743
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 743
aaatgtcact gccggcgaa 19
<210> 744
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 744
ccatgttcta cggcaggcta g 21
<210> 745
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 745
aaaaaggaac tgtggggaat ctgta 25
<210> 746
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 746
ccaagttctt tttgttttac tttattttag cattt 35
<210> 747
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 747
aagcatcata gttactggaa atttgagttt 30
<210> 748
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 748
taaacaaacc tgccaactga agaaattata 30
<210> 749
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 749
gcttcccaaa ttcagatgtt atacttactt 30
<210> 750
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 750
agtatcaata cccacctgtg tcatg 25
<210> 751
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 751
ccgtttttac ctcaggcggt 20
<210> 752
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 752
gaggggaagc tgaagtgcg 19
<210> 753
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 753
cagcctcttc acgcccg 17
<210> 754
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 754
cgtcgtctct gcagccg 17
<210> 755
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 755
ggctttgagg catgctttgg 20
<210> 756
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 756
cgtgcacagc cagaccat 18
<210> 757
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 757
agtgcataca tacctggcct tg 22
<210> 758
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 758
atagaagctg cctaactcgg aaatt 25
<210> 759
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 759
agtgcataca tacctggcct tg 22
<210> 760
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 760
atagaagctg cctaactcgg aaatt 25
<210> 761
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 761
aaccttggag gattcttttt ctctga 26
<210> 762
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 762
ccatcctgtg ctgcttacca t 21
<210> 763
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 763
agggagacgg tgagatgagg 20
<210> 764
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 764
ggtgctctcc tgaacagatg g 21
<210> 765
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 765
cttcttcact ctagcccctt tgtt 24
<210> 766
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 766
actccaggtg gaatgtaact caaaa 25
<210> 767
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 767
cagcccaccc acttgttgt 19
<210> 768
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 768
tacctctttt cctccagtaa ttgttgt 27
<210> 769
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 769
ggtggattgg ctgcttttgt c 21
<210> 770
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 770
gaggcagaat atgtatcggg gatatag 27
<210> 771
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 771
ggtattgcca accttctttg tctataag 28
<210> 772
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 772
tagggtagag atacagcatt ttccttg 27
<210> 773
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 773
ggaggagctg agtctggatc t 21
<210> 774
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 774
cagtgtatag ggggcacagt tc 22
<210> 775
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 775
tgcagaggct tatcttgaac tcc 23
<210> 776
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 776
gctcctttag tttcagtaat tccagtatg 29
<210> 777
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 777
agactgcctc aggagatccc 20
<210> 778
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 778
cataagtatt tgttaccgcc actattatat aagt 34
<210> 779
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 779
gaatgtgcag gtcagctgag a 21
<210> 780
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 780
agttttccaa tacacacttg tcacaaa 27
<210> 781
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 781
gaatgtgcag gtcagctgag a 21
<210> 782
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 782
agttttccaa tacacacttg tcacaaa 27
<210> 783
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 783
gaatgtgcag gtcagctgag a 21
<210> 784
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 784
agttttccaa tacacacttg tcacaaa 27
<210> 785
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 785
tttgaagcgc tctactcgga c 21
<210> 786
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 786
tgctaggaga cccctctgc 19
<210> 787
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 787
atccatcttc aagagagaaa gaatttcatg 30
<210> 788
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 788
gatcctatcc aacccttgtg attca 25
<210> 789
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 789
ccctacatgg atgtggtcag tg 22
<210> 790
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 790
ggccgtccag gagaaagc 18
<210> 791
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 791
gccagggtta gaactcctgc 20
<210> 792
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 792
ttttaattga aaccaaggcc atgct 25
<210> 793
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 793
gctggctgac ttctgtctgt t 21
<210> 794
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 794
cgcccttttt cttttctatg tgca 24
<210> 795
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 795
cacatggaat cagtgatccg tatct 25
<210> 796
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 796
ggttcctgtg actttgggga g 21
<210> 797
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 797
aaagtctttg ccttctacac cttttaatt 29
<210> 798
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 798
caacttaaca gcttgtttcc tggtt 25
<210> 799
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 799
tcctgggtct cctgcaaata atg 23
<210> 800
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 800
tcactctatc tacagatatc tctctggg 28
<210> 801
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 801
attgggataa tgagattttc tacattttag gtt 33
<210> 802
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 802
atccaacatg agtaaaggga tcctg 25
<210> 803
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 803
ctatcccaga gagcaatgat ggg 23
<210> 804
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 804
ccaatgctta ttttatgact cgggag 26
<210> 805
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 805
gcagcgtcgt tgagcct 17
<210> 806
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 806
gcagacgtgg gttaccgg 18
<210> 807
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 807
gacagtgaag agtgcctatg gg 22
<210> 808
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 808
tgctattaag tatctttgag ttagaatttg gaat 34
<210> 809
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 809
gaggggaggc tattctgctt aatt 24
<210> 810
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 810
aaaaatttta ttgccataaa ttaatccagt aggt 34
<210> 811
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 811
gagttattcc tactgcccaa ttttctg 27
<210> 812
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 812
tctttctgaa gctgtaacag taccaa 26
<210> 813
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 813
aagagatgtg aaaacaaact caggttc 27
<210> 814
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 814
ctcgccttaa gccagtcttc at 22
<210> 815
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 815
ttaaagaaaa agaaccttgt taattcagtg c 31
<210> 816
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 816
cagatgagtc tagggttaca aagagg 26
<210> 817
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 817
acactctcgg aaaaatgggg g 21
<210> 818
<211> 16
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 818
gcacctccgg acgtgc 16
<210> 819
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 819
ctgggctttt caagattctg ctaag 25
<210> 820
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 820
cggcctggtc catgtttatg a 21
<210> 821
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 821
cagatgactc ccccttttcg g 21
<210> 822
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 822
ataaaattgt gcagtagagg ttgagattc 29
<210> 823
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 823
agctgacagg gtgaagttta cttt 24
<210> 824
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 824
cctcatggct ttggagagtc c 21
<210> 825
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 825
atactcccag ccgagaagct 20
<210> 826
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 826
actacacagt gggaccaaaa cc 22
<210> 827
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 827
ccagcccagg taagctttca t 21
<210> 828
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 828
cacccccaca gcatcacc 18
<210> 829
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 829
ggggaagatt tgggatattt ctacca 26
<210> 830
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 830
actaaccttc cagtcaaaca tctcc 25
<210> 831
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 831
catgcagcaa tcatgtaagt cataaaataa a 31
<210> 832
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 832
atcatgaatg tgatatggcc ttaaaagg 28
<210> 833
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 833
cccttcctta cataatattt gtctaaaagt ctt 33
<210> 834
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 834
caacatgctc tccgcggt 18
<210> 835
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 835
acactaacag tttgccaggg g 21
<210> 836
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 836
acataccctg cagaagaccc t 21
<210> 837
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 837
gaaaggaagc ggagagatgt cat 23
<210> 838
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 838
gttagaaatg acagttctct ccttgttatc 30
<210> 839
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 839
ttttgacctg aaacagccga ga 22
<210> 840
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 840
ggccatgtag aaaaagagga atgc 24
<210> 841
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 841
aaagggaatg gttatgattt ttctacctg 29
<210> 842
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 842
gttcttcaat caaggtcagt gctg 24
<210> 843
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 843
gcgaccccag atgaaggtg 19
<210> 844
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 844
ccgatcataa ctgtggcaga aga 23
<210> 845
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 845
gagcccaaac atgctgttca tc 22
<210> 846
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 846
gatcctctcc agaaacttgg acc 23
<210> 847
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 847
cttcctgagc ttgtgtggat tatttc 26
<210> 848
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 848
gactccaggg tgcagaagat g 21
<210> 849
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 849
cttcctgagc ttgtgtggat tatttc 26
<210> 850
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 850
gactccaggg tgcagaagat g 21
<210> 851
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 851
gtcgctctgc aggaagtcc 19
<210> 852
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 852
caccaccaca aaagcgtaag tg 22
<210> 853
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 853
cctgcagagc ctcacgc 17
<210> 854
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 854
cttccctgca gcctgagatg 20
<210> 855
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 855
ccaggggctt attcacttga aataac 26
<210> 856
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 856
atcactcagc aacatagggc ag 22
<210> 857
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 857
tggtgaaaag cctccaccac 20
<210> 858
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 858
tttctggaag aacctgagtt ggg 23
<210> 859
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 859
gactcattgc tctttccacc aatg 24
<210> 860
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 860
aaaagccaac cctgcacaga 20
<210> 861
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 861
aaaagacttg aggaacagga aaaagattta 30
<210> 862
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 862
gaaaaaccct ataaatgcaa agaatgtgg 29
<210> 863
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 863
gcctgaccac attctctgct aa 22
<210> 864
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 864
cttctgtgtt ctctctccag atactg 26
<210> 865
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 865
catgctgtac ttctggttct ctga 24
<210> 866
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 866
tgctattgct ccagcttagt tctt 24
<210> 867
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 867
catgctgtac ttctggttct ctga 24
<210> 868
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 868
tgctattgct ccagcttagt tctt 24
<210> 869
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 869
ggaacccttc agccttgagg 20
<210> 870
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 870
ttgtatagtt tgttgctact agaaattctg g 31
<210> 871
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 871
gtcactcctc tttctagaac agtctc 26
<210> 872
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 872
gagtcactgc ccatggtcat t 21
<210> 873
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 873
gtcactcctc tttctagaac agtctc 26
<210> 874
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 874
gagtcactgc ccatggtcat t 21
<210> 875
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 875
tttaccgttt aattcttaaa tatacacaga aacac 35
<210> 876
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 876
atgacctgca tattatagat ttgtgaaatt ttaag 35
<210> 877
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 877
caccaggtag gaagcgctg 19
<210> 878
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 878
tcactgatgt atcccaagca catag 25
<210> 879
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 879
agacagtgat aaagaatgga tcatttgc 28
<210> 880
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 880
ttagttcagc ctatttaaaa gtaagaaaag tca 33
<210> 881
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 881
gggccgttcc actgtgtc 18
<210> 882
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 882
tccttccgga agcaaatcta agtc 24
<210> 883
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 883
cctgtcattg gggcagacc 19
<210> 884
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 884
tctctgccct gcctctctc 19
<210> 885
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 885
gggctccacc agacatgc 18
<210> 886
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 886
agggtctgat aatcctgctc ca 22
<210> 887
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 887
taagaagtgc cggtcctgaa taag 24
<210> 888
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 888
ctctacattg cctacagtga cgaa 24
<210> 889
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 889
tgcagggaca gagtgacca 19
<210> 890
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 890
tgggaggtgc actgctct 18
<210> 891
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 891
aggccacccc tagatgacc 19
<210> 892
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 892
gttaacaata tccttgacag tgctgg 26
<210> 893
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 893
tgcctctctc tgctcagata ca 22
<210> 894
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 894
caggggattg gagaaccgaa tt 22
<210> 895
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 895
actgtggtga gtatccccct ag 22
<210> 896
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 896
ctccgtcctt cagtcttggc 20
<210> 897
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 897
cacaagacta ttgctccacc ca 22
<210> 898
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 898
ttcaatggga actgtatgaa ggtgata 27
<210> 899
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 899
ccatgaccag gccatcagtg 20
<210> 900
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 900
cgagtgaatg gcagtcacag at 22
<210> 901
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 901
gaggcagagt gattgagaca gg 22
<210> 902
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 902
ggggcccaca gattggc 17
<210> 903
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 903
cacccagggg acgtgtaaag 20
<210> 904
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 904
gcccccacca ccatgatg 18
<210> 905
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 905
cacccagggg acgtgtaaag 20
<210> 906
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 906
gcccccacca ccatgatg 18
<210> 907
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 907
cctgtaccgg cagagcatc 19
<210> 908
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 908
gagcgggtga gacttagcg 19
<210> 909
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 909
agggactgaa tgcatgcctg 20
<210> 910
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 910
ctgtgtatct gagaacggtc atgt 24
<210> 911
<211> 16
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 911
ccttgcagag gcggcc 16
<210> 912
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 912
aggagagtca ccaagccagt 20
<210> 913
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 913
attcttagat tactaaaggg attggggtt 29
<210> 914
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 914
gcagggagtg gtaagagttc ag 22
<210> 915
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 915
tttcattgtt tgagctgttg tccaata 27
<210> 916
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 916
gtacaggatg aggagtttaa agactattct a 31
<210> 917
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 917
tgaattttga agttaatata ttcacgaaga gga 33
<210> 918
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 918
tgcattagtt agcttgagtc tctcc 25
<210> 919
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 919
agcattcatg acttttgcag acg 23
<210> 920
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 920
aatagggcta gatttggaaa attcttttag taa 33
<210> 921
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 921
attccaaacc aaaagaagca agca 24
<210> 922
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 922
gcggagagga gtcctgagt 19
<210> 923
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 923
ggagacacgc tggagtaaag g 21
<210> 924
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 924
ccacatctct gagctaaata taccctag 28
<210> 925
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 925
ataaatcctg ggaagttatt actaagcctt a 31
<210> 926
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 926
cagcttcatg gggctctgg 19
<210> 927
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 927
tgtaataacg cccactcaga agg 23
<210> 928
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 928
taaatagaga taaatacatg ggatggggag 30
<210> 929
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 929
accttgagga aagtgaaagc tgt 23
<210> 930
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 930
ccagccaaat cctcagagtc c 21
<210> 931
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 931
gtagtatgag ttccatcaag gtgct 25
<210> 932
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 932
ggctatcctc tcagagtgac atttt 25
<210> 933
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 933
ctgagctgga tctttacgtc gt 22
<210> 934
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 934
aggggctggg gagaatcc 18
<210> 935
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 935
ctgagctgga tctttacgtc gt 22
<210> 936
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 936
aggggctggg gagaatcc 18
<210> 937
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 937
aagaaactgg actttggcca ct 22
<210> 938
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 938
tggaagatac attttgttat tggtgctaat 30
<210> 939
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 939
cctataacaa acctataacc tttgtcttga g 31
<210> 940
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 940
ctttgagttc ataggtcctc aggag 25
<210> 941
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 941
ggagcaaaga gttagggaga cg 22
<210> 942
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 942
atccccgtct ctctgtacct g 21
<210> 943
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 943
ttgcgctagc tccttggaag 20
<210> 944
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 944
cctagcctcc ctataaataa atgaggag 28
<210> 945
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 945
tctggagaac cacactgagg t 21
<210> 946
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 946
tcacacttgt tgtcaggccc 20
<210> 947
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 947
tctggagaac cacactgagg t 21
<210> 948
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 948
tcacacttgt tgtcaggccc 20
<210> 949
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 949
ggtgtaggcg gttgggc 17
<210> 950
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 950
cgcaagacgt gacatagtct ga 22
<210> 951
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 951
cgtgggtgag tgcgcat 17
<210> 952
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 952
acagcaatcc cggaatccac 20
<210> 953
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 953
ctagactaaa ccccaactgg ctc 23
<210> 954
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 954
agtcatttaa gctccccaag tcc 23
<210> 955
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 955
ctagactaaa ccccaactgg ctc 23
<210> 956
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 956
agtcatttaa gctccccaag tcc 23
<210> 957
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 957
tacccttggg cctcggg 17
<210> 958
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 958
cagcattagc aaaccttggc tc 22
<210> 959
<211> 16
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 959
ccctgtgccg cggaag 16
<210> 960
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 960
cggcgcagta ccaggtg 17
<210> 961
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 961
aaagagacct aataagctca ggtagttc 28
<210> 962
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 962
ctgctggaga ggtagtgagg a 21
<210> 963
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 963
tggctttgtg atcccagata tcatt 25
<210> 964
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 964
ttggcataat tacagaaaat aaagaacatg c 31
<210> 965
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 965
atagcaagat cctgtctcta ttttcaaaaa taa 33
<210> 966
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 966
cagttttcag gttgggaaaa tattaattga g 31
<210> 967
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 967
ttggcatacc ttgaataaga tttcacg 27
<210> 968
<211> 32
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 968
aacatcaagt ataatgtggg tgtatttaat gt 32
<210> 969
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 969
gaaataatta tgttcttacc tgggccatt 29
<210> 970
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 970
atgagtgttt taataaggaa acctatgctc 30
<210> 971
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 971
tatctgtaat ctgaaacagg ttaagaggta ata 33
<210> 972
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 972
atgtttgtga cagttgaact aacacc 26
<210> 973
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 973
actgcaagca tcttaaactc taatgc 26
<210> 974
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 974
gtttctttct cttcttttat atactttgca tcatt 35
<210> 975
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 975
tcccactcag acggaccc 18
<210> 976
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 976
aggggtgaag gaggaggtg 19
<210> 977
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 977
ctgacctacc actcacctcc a 21
<210> 978
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 978
ggaggaggga ccagagttgt 20
<210> 979
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 979
ctgacctacc actcacctcc a 21
<210> 980
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 980
ggaggaggga ccagagttgt 20
<210> 981
<211> 16
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 981
gctgccttcc accccg 16
<210> 982
<211> 16
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 982
ctggccgctg tgcctt 16
<210> 983
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 983
agaatgggcc gtctgggta 19
<210> 984
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 984
ggctggggtt tggagacttt 20
<210> 985
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 985
ctgtttctcg tagatgtcca cgt 23
<210> 986
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 986
aaggagtgag tgctcttttt cctt 24
<210> 987
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 987
aaggcgccct gctccta 17
<210> 988
<211> 15
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 988
ggcggggttt ggggc 15
<210> 989
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 989
ctctctcggc tggcaacg 18
<210> 990
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 990
gtggtggttt ttgttcggtg tag 23
<210> 991
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 991
cagagccatg tgtccaggac 20
<210> 992
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 992
attgtagtga aagaggactc gctc 24
<210> 993
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 993
acgggtggcc tcatcga 17
<210> 994
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 994
ggcctccgct ttttccga 18
<210> 995
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 995
ggttggctca gtctcaagca 20
<210> 996
<211> 16
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 996
tggggagcct ggggtg 16
<210> 997
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 997
gagccacata cctaagcacc ag 22
<210> 998
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 998
cattcttcca gtccattctc tctagtc 27
<210> 999
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 999
gcacttttct tccacgatgc c 21
<210> 1000
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1000
ttaaatcaga ctttggatga agatttccta c 31
<210> 1001
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1001
ctcctctttg ttgtctccta cctg 24
<210> 1002
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1002
atgtccgtgc tgtggctg 18
<210> 1003
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1003
ggggctgtcg aacgagc 17
<210> 1004
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1004
aaggacctgg cgtatcgatt tc 22
<210> 1005
<211> 31
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1005
tcaaaatgaa gcagaagtaa agaaactaag a 31
<210> 1006
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1006
ttgtctgtgt aatcttattt ccttactctt ttt 33
<210> 1007
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1007
cgggggcttg ggaggta 17
<210> 1008
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1008
agcaggccat gggacct 17
<210> 1009
<211> 15
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1009
gcagctgcgc ggaca 15
<210> 1010
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1010
agtgcacagg taggggagg 19
<210> 1011
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1011
acacatttgc atgcacacac ag 22
<210> 1012
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1012
gtgcatgttt actagcagga cct 23
<210> 1013
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1013
ctcctcatga cagctgccat t 21
<210> 1014
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1014
ctgcagcatt tgacattggg ag 22
<210> 1015
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1015
gaagggctcc cggacaatg 19
<210> 1016
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1016
ctcattatcc accacgcaca ga 22
<210> 1017
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1017
gctctggagg taggctgagt a 21
<210> 1018
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1018
ggacgaacac tcccagatct tc 22
<210> 1019
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1019
ctggcctgct gcacctag 18
<210> 1020
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1020
tgtttcctac agatctgctt ggg 23
<210> 1021
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1021
tcaaagcctt gctggtcttg t 21
<210> 1022
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1022
gagagcggtt gcccagtc 18
<210> 1023
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1023
gcagaaatcc cgctcgagaa 20
<210> 1024
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1024
ggcaccaatg gagaggagc 19
<210> 1025
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1025
ccgctaccct tgcaccg 17
<210> 1026
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1026
cgctgagcct gtggtcg 17
<210> 1027
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1027
cagtagtggg agctgtcttc atc 23
<210> 1028
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1028
gcatgtgtgt gtatgtcagt gatg 24
<210> 1029
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1029
gacagcacag actcagggc 19
<210> 1030
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1030
atcaaccgct acgactgtgt c 21
<210> 1031
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1031
aattgttggg tgtttcctta ggga 24
<210> 1032
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1032
caaggtccga gctgcctg 18
<210> 1033
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1033
gatgctcaat tactcatcag acatcg 26
<210> 1034
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1034
aaccctaaaa tgcgaagagg agtaa 25
<210> 1035
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1035
gccactgaac tcatccgct 19
<210> 1036
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1036
atcctctaaa atcctcaatt ggcct 25
<210> 1037
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1037
gccactgaac tcatccgct 19
<210> 1038
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1038
atcctctaaa atcctcaatt ggcct 25
<210> 1039
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1039
gccactgaac tcatccgct 19
<210> 1040
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1040
atcctctaaa atcctcaatt ggcct 25
<210> 1041
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1041
aagtcccagg ggagatggaa a 21
<210> 1042
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1042
tttcccagga ttgagagggg t 21
<210> 1043
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1043
aagtcccagg ggagatggaa a 21
<210> 1044
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1044
tttcccagga ttgagagggg t 21
<210> 1045
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1045
aatggtaagc ctcgtccttg tc 22
<210> 1046
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1046
caggaaagtg gtgggatctg g 21
<210> 1047
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1047
ccctcctctt ctccccagg 19
<210> 1048
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1048
gaggtcaagg ctgccatcc 19
<210> 1049
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1049
agtcattcaa gctctatcca agaagtta 28
<210> 1050
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1050
agaggggtgg ggatgtgag 19
<210> 1051
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1051
gactgagatc ttaggctttg ggg 23
<210> 1052
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1052
ctggactctc agattggaga agatg 25
<210> 1053
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1053
ccctcagacc cacgagtcta 20
<210> 1054
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1054
tgtcactccc catggcct 18
<210> 1055
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1055
gtgtgatgta tcttgatgag atagaatgc 29
<210> 1056
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1056
ctagggaggc tgacgtgaga 20
<210> 1057
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1057
gtggcagaaa aagtgatagg ttacaaa 27
<210> 1058
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1058
tgtcattcca tagtgtatgc gtacat 26
<210> 1059
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1059
atgtctttcc agtgccactg tt 22
<210> 1060
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1060
aaagattatg tttagtgtag gtatgtttcc aatat 35
<210> 1061
<211> 32
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1061
gaaaaacaca tttcaaaaag aagcaatatg ag 32
<210> 1062
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1062
ttcttgtatt gtccacaaaa atagaactat aca 33
<210> 1063
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1063
tcttggagtg gttgaaaaga tactgt 26
<210> 1064
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1064
acttttccac tagcttcttc aggtt 25
<210> 1065
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1065
cgaatcccac tcttcctcct tg 22
<210> 1066
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1066
ctgcagcagg aacggagg 18
<210> 1067
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1067
ttctgggtgc tgttactaca caaa 24
<210> 1068
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1068
cacctcactg ctcacgaaat aaac 24
<210> 1069
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1069
cccgcttctt ccctaacgg 19
<210> 1070
<211> 16
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1070
gccgaggctg ccatcc 16
<210> 1071
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1071
tcaggtcttt tccagaaagc aca 23
<210> 1072
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1072
tgaagcaatt ctctccatta tgttgc 26
<210> 1073
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1073
ctgaagctga aagaagggcc a 21
<210> 1074
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1074
tggcatcctg actccttgag a 21
<210> 1075
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1075
ggggcagaat cactccgtg 19
<210> 1076
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1076
attcctgtgt gcacgtgtgt 20
<210> 1077
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1077
ctcttggagg taaactgtcc cg 22
<210> 1078
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1078
tgccatagtc acttgtgaat gagtat 26
<210> 1079
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1079
gaaggaaata ttacagggga cgct 24
<210> 1080
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1080
cacagggaaa caagttgcag tg 22
<210> 1081
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1081
gtgaatggtc ccagcctcg 19
<210> 1082
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1082
agaccccagg gattagggc 19
<210> 1083
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1083
ctgacacagg agctgaagga c 21
<210> 1084
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1084
cgggcaaagg tgtgggaa 18
<210> 1085
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1085
ccccacattc acctccacg 19
<210> 1086
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1086
gtgagattct gcgtgtaaag ttcg 24
<210> 1087
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1087
tgggggagtg gttggca 17
<210> 1088
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1088
cttctcctag aaccttctcc atgc 24
<210> 1089
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1089
ccagggagtg cagaaccaaa t 21
<210> 1090
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1090
ccagtgctga ggatggcg 18
<210> 1091
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1091
gacggcaatg ctgatggga 19
<210> 1092
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1092
ttgggggcca atacatgatg ag 22
<210> 1093
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1093
gaggcgtctg gacaagctg 19
<210> 1094
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1094
ctgcgccttc acgtcgaa 18
<210> 1095
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1095
gcccaattcc ctccttgct 19
<210> 1096
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1096
gggagggctt cggagga 17
<210> 1097
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1097
ttaagactcg cagcactgga tc 22
<210> 1098
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1098
cacatcccca gagaactgtc g 21
<210> 1099
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1099
gcccaactca ccttatgggg 20
<210> 1100
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1100
tttgcacaac tttgtcatgg acc 23
<210> 1101
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1101
ggacgagctg gtcaaggtg 19
<210> 1102
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1102
cgtgcctggc atccttgt 18
<210> 1103
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1103
gccaccctct gtgcacc 17
<210> 1104
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1104
gcatctggct gtggctgt 18
<210> 1105
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1105
gccaccctct gtgcacc 17
<210> 1106
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1106
gcatctggct gtggctgt 18
<210> 1107
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1107
caccgctcaa cgtgctct 18
<210> 1108
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1108
agaaatgaca ttgagctggt ctga 24
<210> 1109
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1109
acgacttgca gagcatccg 19
<210> 1110
<211> 15
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1110
ctgcggctgg ccacc 15
<210> 1111
<211> 15
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1111
gggcgcccac cgaag 15
<210> 1112
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1112
tcggtagcct ttaggtccga ta 22
<210> 1113
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1113
acacctgccc cagtgagt 18
<210> 1114
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1114
ccctggtgat gcagccg 17
<210> 1115
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1115
ggcgaccact gtagcttcc 19
<210> 1116
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1116
atcctcacgt cctcccatga t 21
<210> 1117
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1117
tccctgaatg cttccatatg gc 22
<210> 1118
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1118
acaaacagca tgagcaggac a 21
<210> 1119
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1119
tccctgaatg cttccatatg gc 22
<210> 1120
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1120
acaaacagca tgagcaggac a 21
<210> 1121
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1121
atgaagaaag ggagagattt gggg 24
<210> 1122
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1122
aaagcagggt cgcatggg 18
<210> 1123
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1123
ctggaagtca gtcgaacata ttttcag 27
<210> 1124
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1124
agagcacaaa accaaggacc at 22
<210> 1125
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1125
ctggaagtca gtcgaacata ttttcag 27
<210> 1126
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1126
agagcacaaa accaaggacc at 22
<210> 1127
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1127
gcagctgagg catttaaagg tg 22
<210> 1128
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1128
ctgatgctaa tggaaaggca cattt 25
<210> 1129
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1129
gcagctgagg catttaaagg tg 22
<210> 1130
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1130
ctgatgctaa tggaaaggca cattt 25
<210> 1131
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1131
gcagctgagg catttaaagg tg 22
<210> 1132
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1132
ctgatgctaa tggaaaggca cattt 25
<210> 1133
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1133
cctccagaga ccatagctcc a 21
<210> 1134
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1134
aagaaagaaa ggccacttcc gt 22
<210> 1135
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1135
cctccagaga ccatagctcc a 21
<210> 1136
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1136
aagaaagaaa ggccacttcc gt 22
<210> 1137
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1137
cctccagaga ccatagctcc a 21
<210> 1138
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1138
aagaaagaaa ggccacttcc gt 22
<210> 1139
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1139
cctccagaga ccatagctcc a 21
<210> 1140
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1140
aagaaagaaa ggccacttcc gt 22
<210> 1141
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1141
tggcgggaac aggaggat 18
<210> 1142
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1142
gtccagccct cttgaagctc 20
<210> 1143
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1143
aacatgtccc agacacgcag 20
<210> 1144
<211> 16
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1144
tcctgcagcc cacggt 16
<210> 1145
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1145
ggtactgcgg agacaactgt ag 22
<210> 1146
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1146
agagagagtg tcatcctcgg g 21
<210> 1147
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1147
cctggacaga ggagaggagg 20
<210> 1148
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1148
catgagaatg tcagctcctt gaga 24
<210> 1149
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1149
cctggacaga ggagaggagg 20
<210> 1150
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1150
catgagaatg tcagctcctt gaga 24
<210> 1151
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1151
cttgtggttc cttctaggct acc 23
<210> 1152
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1152
gcgctatagt tacaaagata gtttcgg 27
<210> 1153
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1153
tgcgggcaga caccaattta 20
<210> 1154
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1154
tttacagctt ctaacacatt tatgtttcct 30
<210> 1155
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1155
tcaatctctg aaggttttgg gca 23
<210> 1156
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1156
tagaaacata gtgcttatct tcccagg 27
<210> 1157
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1157
tggtctattc cgtgagaccg a 21
<210> 1158
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1158
gtccagcctc gggtttgg 18
<210> 1159
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1159
ggtggcatgc ttcaggct 18
<210> 1160
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1160
gatcctgtct ctaaagcact ttaaaagag 29
<210> 1161
<211> 18
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1161
ggtggcatgc ttcaggct 18
<210> 1162
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1162
gatcctgtct ctaaagcact ttaaaagag 29
<210> 1163
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1163
cccgggagat cagattgtaa tgatt 25
<210> 1164
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1164
tttcaaccat gtgtatgtgt tggc 24
<210> 1165
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1165
ctacaagttc caccagcatt gttc 24
<210> 1166
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1166
ccttgagggc atgaagggta taaa 24
<210> 1167
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1167
gctatctgtc cccggacct 19
<210> 1168
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1168
cacatctgcc tccatctcag tc 22
<210> 1169
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1169
tattgtttca tctgaaaggc agtagtaaag 30
<210> 1170
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1170
atggataact cacagagacc agttg 25
<210> 1171
<211> 26
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1171
agtttgccac taccatttaa aaagca 26
<210> 1172
<211> 28
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1172
acattttctg gaagtcaaac gtcaataa 28
<210> 1173
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1173
ctggccactc ttctgatcag tc 22
<210> 1174
<211> 17
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1174
gccctcagcg tgacacc 17
Claims (8)
1.一种激素性股骨头坏死易感基因panel，其为包括下述587个人类基因组位点的基因集：
rs710322、rs2294486、rs2294487、rs150880809、rs7407、rs144487103、rs12738235、rs10909811、rs78132938、rs11121552、rs9435659、rs761421、rs2076603、rs4920608、rs67232976、rs6666034、rs2064374、rs2295942、rs3748705、rs600674、rs12568483、rs74843031、rs10493845、rs78167983、rs4658219、rs147504208、rs79090643、rs6424379、rs11579366、rs2004659、rs9728345、rs2231375、rs2280474、rs4971066、rs17472444、rs7550383、rs10919082、rs4656656、rs4656657、rs7526721、rs10800420、rs3748618、rs2274227、rs3795503、rs10797850、rs1256930、rs10900525、rs11120575、rs1059704、rs2297545、rs2297543、rs2076149、rs9435867、rs7539016、rs7549137、rs7551511、rs2049182、rs10489691、rs3795375、rs3795374、rs963982、rs963981、rs4659948、rs562038978、rs111896385、rs1056104、rs7560262、rs58882124、rs200809231、rs187904341、rs937725、rs12233086、rs11691981、rs777432、rs12052654、rs13025791、rs13025959、rs2255161、rs11686946、rs35720878、rs2124971、rs2272499、rs16860497、rs2293649、rs56084828、rs2305414、rs1046356、rs2287600、rs13021295、rs59458664、rs3214826、rs2305541、rs2290464、rs2924811、rs2971863、rs1470864、rs2167885、rs78122518、rs72982319、rs143258246、rs838543、rs838549、rs78451449、rs151152774、rs79471829、rs117751285、rs200139615、rs3790993、rs200039497、rs456168、rs6796483、rs6777976、rs61743511、rs61743461、rs2070987、rs1546737、rs10933973、rs7614116、rs75144949、rs3732755、rs12492699、rs150242411、rs583550、rs35070271、rs55683908、rs843358、rs843357、rs11924016、rs371821065、rs6762208、rs1823238、rs6804448、rs138704337、rs28434055、rs322117、rs11355796、rs16844401、rs75501914、rs730420、rs2269877、rs62289296、rs62289297、rs4320134、rs3755863、rs8192678、rs11940551、rs6837020、rs77939156、rs4031826、rs2219471、rs3214870、rs3816584、rs2305946、rs7655964、rs1870377、rs7349683、rs3836713、rs861340、rs13128286、rs3966087、rs2175766、rs61758879、rs2241894、rs13147012、rs6847454、rs1039808、rs3756122、rs17024037、rs12650966、rs2289318、rs12648678、rs29675、rs1126417、rs162850、rs162848、rs332811、rs37368、rs4371784、rs3797209、rs1423099、rs10070440、rs423906、rs401302、rs3749669、rs59653731、rs10477486、rs6888031、rs11283943、rs2227950、rs2303076、rs2306960、rs3812036、rs70997617、rs3817064、rs3817063、rs75304543、rs368030749、rs372469036、rs778456436、rs1749158、rs2842949、rs6239、rs11754288、rs13204445、rs3749877、rs3749878、rs76902576、rs80125253、rs2297740、rs7762830、rs12435、rs1132742、rs1131123、rs1131500、rs1140546、rs1051488、rs3808343、rs117545108、rs4720537、rs4222、rs67361882、rs2286213、rs4721888、rs73705179、rs78155900、rs114097201、rs367830344、rs375743758、rs4646453、rs71540916、rs71540918、rs1424426、rs12671813、rs2278130、rs2069459、rs2069456、rs72494453、rs61013791、rs7829535、rs658948、rs2280335、rs35858677、rs145012061、rs34462909、rs17092126、rs1078363、rs6996616、rs2278467、rs1042381、rs10956412、rs17056759、rs6435、rs6397、rs12549574、rs11793555、rs7853758、rs2027433、rs3737309、rs2015408、rs2275137、rs10796042、rs2282383、rs6602051、rs7100510、rs1954181、rs12256835、rs2297329、rs2297328、rs11008968、rs148671234、rs138210257、rs10827116、rs2230396、rs2230395、rs2230394、rs2660169、rs735877、rs3736583、rs3736582、rs7912404、rs4986789、rs7894、rs4575219、rs3739968、rs6578504、rs11603496、rs3740620、rs11605894、rs11605072、rs398075678、rs96489、rs331510、rs2657167、rs10836954、rs11037444、rs60832895、rs11037445、rs16930982、rs16930998、rs55802517、rs12272856、rs1043388、rs1043390、rs1128396、rs2072651、rs10458926、rs10833050、rs10766524、rs2280331、rs369353409、rs574381、rs626670、rs2286163、rs200951888、rs57503021、rs7932320、rs7130258、rs66861805、rs7950735、rs291241、rs7931870、rs2186627、rs12790613、rs503223、rs1893764、rs79715120、rs79669172、rs77206991、rs79417294、rs114979979、rs75994039、rs75240316、rs59358830、rs57751948、rs775990209、rs2075626、rs58283839、rs2241281、rs2970827、rs2241280、rs2241279、rs12426675、rs1800692、rs11542844、rs2273986、rs7138535、rs4763232、rs7135018、rs12313469、rs10772423、rs12370363、rs78152338、rs2708381、rs2600357、rs2600356、rs2599404、rs2600355、rs11557132、rs61928643、rs9943714、rs9943809、rs2280446、rs6581565、rs825074、rs10861953、rs4072888、rs12821855、rs73166888、rs1451772、rs1817104、rs1669412、rs9315906、rs7982426、rs520342、rs3092904、rs12429818、rs7331894、rs1805097、rs2275841、rs2228036、rs1713417、rs2297612、rs4981349、rs74036552、rs150516189、rs1808975、rs2235962、rs3742885、rs181572、rs75391113、rs60593979、rs2180770、rs3837659、rs3829955、rs7153601、rs11626446、rs17127245、rs4401027、rs60585083、rs11366248、rs2260160、rs2467426、rs12441861、rs2277531、rs3759791、rs12702、rs2228368、rs3736180、rs8023508、rs12592155、rs28706938、rs28673176、rs2277598、rs57373048、rs17875502、rs397954069、rs28504011、rs11259927、rs2304796、rs3743262、rs10664668、rs4646626、rs3832984、rs12447306、rs2667661、rs2667660、rs2240691、rs2302607、rs115465506、rs1050113、rs14122、rs11865800、rs11076125、rs11076176、rs12691052、rs9928398、rs2241619、rs2241620、rs58353328、rs3803640、rs11557187、rs2077412、rs2663345、rs2286672、rs10999、rs3744400、rs16956647、rs11078698、rs76317718、rs148830167、rs61274670、rs2074274、rs2074273、rs2074272、rs111232194、rs2074146、rs4796030、rs2074519、rs3744372、rs2304967、rs143471015、rs1719152、rs16532、rs2227322、rs25645、rs4072639、rs2070833、rs756100328、rs79526563、rs2301682、rs143818567、rs2285524、rs4793639、rs3216895、rs2301627、rs2301625、rs3785925、rs2075555、rs2075554、rs2256835、rs2665799、rs2302133、rs2302134、rs10605889、rs7208382、rs11077414、rs9909216、rs12941264、rs690371、rs2467577、rs2070919、rs690514、rs2070918、rs1688149、rs492095、rs35286723、rs2306690、rs552432、rs2305027、rs2240900、rs7243349、rs17851892、rs7234849、rs3763951、rs713041、rs35870594、rs10421267、rs11671881、rs8120、rs2292151、rs7255265、rs2240745、rs2277969、rs35647251、rs137943075、rs7245563、rs2056820、rs56156262、rs36008189、rs12459147、rs34433978、rs3833221、rs305968、rs12975986、rs34650210、rs305975、rs73039860、rs1799782、rs17725124、rs2547238、rs45468391、rs8100114、rs4802905、rs12609379、rs12977654、rs56960413、rs862708、rs78628129、rs398059497、rs41275620、rs6133723、rs2295576、rs1078761、rs6030462、rs3746504、rs3737063、rs3918254、rs13969、rs140429049、rs8133052、rs1056892、rs11701124、rs8131313、rs8132521、rs35796750、rs881712、rs2839077、rs59769541、rs11464071、rs11089978、rs11089977、rs165657、rs361666、rs361762、rs362003、rs362124、rs362132、rs361611、rs361580、rs362011、rs361721、rs1476576、rs11089442、rs2074731、rs3218318、rs2072862、rs5758651、rs6006950、rs200564038、rs140313、rs372438307、rs376698610、rs6654760、rs2071776、rs2854412、rs6616890、rs143921252。
2.如权利要求1所述的激素性股骨头坏死易感基因panel，其特征在于，用于构建基于多态性位点的GA-ONFH风险预测模型。
3.如权利要求2所述的激素性股骨头坏死易感基因panel，其特征在于，所述GA-ONFH风险预测模型选自：以OR值作为权重的遗传风险评分OR_GRS模型、可释方差遗传风险评分EV_GRS模型。
4.如权利要求3所述的激素性股骨头坏死易感基因panel，其特征在于，所述OR_GRS模型为基于OR_GRS风险评分的单因素逻辑回归分析模型或者基于OR_GRS风险评分的多因素逻辑回归分析模型。
5.如权利要求3所述的激素性股骨头坏死易感基因panel，其特征在于，所述EV_GRS模型为基于EV_GRS风险评分的单因素逻辑回归分析模型或者基于EV_GRS风险评分的多因素逻辑回归分析模型。
6.如权利要求2-5中任一项所述的激素性股骨头坏死易感基因panel，其特征在于，所述GA-ONFH风险预测模型通过编程、通过数学软件包形式被输入计算机或者基因检测仪。
7.一种激素性股骨头坏死易感性检测装置或者试剂盒，其特征在于，包括用于检测如权利要求1中所述基因panel的引物或者DNA/RNA探针。
8.如权利要求7所述的激素性股骨头坏死易感性检测装置或者试剂盒，其特征在于，用于检测所述基因panel中人类基因组位点的引物序列如表2中所示。
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Citations (6)
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