CN111647532A - Probiotic composition for degrading bagasse and preparation method thereof - Google Patents
Probiotic composition for degrading bagasse and preparation method thereof Download PDFInfo
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- 239000006041 probiotic Substances 0.000 title claims abstract description 69
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 69
- 239000000203 mixture Substances 0.000 title claims abstract description 52
- 241000609240 Ambelania acida Species 0.000 title claims abstract description 51
- 239000010905 bagasse Substances 0.000 title claims abstract description 51
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 47
- 230000000593 degrading effect Effects 0.000 title claims abstract description 25
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 238000000855 fermentation Methods 0.000 claims abstract description 51
- 230000004151 fermentation Effects 0.000 claims abstract description 51
- 240000008042 Zea mays Species 0.000 claims abstract description 25
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 25
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 25
- 235000005822 corn Nutrition 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- 240000000111 Saccharum officinarum Species 0.000 claims abstract description 22
- 235000007201 Saccharum officinarum Nutrition 0.000 claims abstract description 22
- 235000009811 Momordica charantia Nutrition 0.000 claims abstract description 19
- 240000006439 Aspergillus oryzae Species 0.000 claims abstract description 11
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims abstract description 11
- 241000186016 Bifidobacterium bifidum Species 0.000 claims abstract description 11
- 241000194032 Enterococcus faecalis Species 0.000 claims abstract description 11
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 11
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 11
- 241000186679 Lactobacillus buchneri Species 0.000 claims abstract description 11
- 241000186605 Lactobacillus paracasei Species 0.000 claims abstract description 11
- 241000191998 Pediococcus acidilactici Species 0.000 claims abstract description 11
- 241000235546 Rhizopus stolonifer Species 0.000 claims abstract description 11
- 229940032049 enterococcus faecalis Drugs 0.000 claims abstract description 11
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims abstract description 11
- 239000002994 raw material Substances 0.000 claims abstract description 11
- 239000011268 mixed slurry Substances 0.000 claims description 44
- 230000001954 sterilising effect Effects 0.000 claims description 18
- 235000008322 Trichosanthes cucumerina Nutrition 0.000 claims description 16
- 238000002156 mixing Methods 0.000 claims description 15
- 238000004140 cleaning Methods 0.000 claims description 10
- 229940002008 bifidobacterium bifidum Drugs 0.000 claims description 9
- 238000006731 degradation reaction Methods 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims description 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 7
- 230000001580 bacterial effect Effects 0.000 claims description 7
- 230000015556 catabolic process Effects 0.000 claims description 7
- 238000002791 soaking Methods 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 claims description 6
- 208000003643 Callosities Diseases 0.000 claims description 6
- 206010020649 Hyperkeratosis Diseases 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 238000004537 pulping Methods 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 244000078912 Trichosanthes cucumerina Species 0.000 claims 4
- 239000000463 material Substances 0.000 claims 1
- 244000302512 Momordica charantia Species 0.000 abstract description 15
- 235000009812 Momordica cochinchinensis Nutrition 0.000 abstract description 3
- 235000018365 Momordica dioica Nutrition 0.000 abstract description 3
- 241000235342 Saccharomycetes Species 0.000 abstract description 2
- 239000000835 fiber Substances 0.000 description 7
- 239000007788 liquid Substances 0.000 description 4
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 229930195730 Aflatoxin Natural products 0.000 description 1
- XWIYFDMXXLINPU-UHFFFAOYSA-N Aflatoxin G Chemical compound O=C1OCCC2=C1C(=O)OC1=C2C(OC)=CC2=C1C1C=COC1O2 XWIYFDMXXLINPU-UHFFFAOYSA-N 0.000 description 1
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
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- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
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- 244000082204 Phyllostachys viridis Species 0.000 description 1
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- 241000607142 Salmonella Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
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- 150000001413 amino acids Chemical class 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
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- 229940088598 enzyme Drugs 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
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- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01K1/0155—Litter comprising organic material
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- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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Abstract
The invention relates to a bagasse degrading probiotic flora composition and a preparation method thereof, wherein the raw material components of the bagasse degrading probiotic flora composition comprise probiotics, corn, balsam pear, sugarcane and water, wherein the probiotics are prepared from saccharomycetes, lactobacillus buchneri, lactobacillus acidophilus, bacillus bifidus, enterococcus faecalis, pediococcus acidilactici, lactobacillus paracasei, rhizopus nigricans and aspergillus oryzae in a specific ratio. The probiotic flora composition can fully decompose bagasse, and the decomposed mixture can be used as high-quality biological feed or fermentation bed padding; the preparation method is simple and easy to operate, low in cost and easy to popularize.
Description
Technical Field
The invention relates to the field of biological fermentation, and in particular relates to a probiotic composition for degrading bagasse and a preparation method thereof.
Background
Sugarcane is one of the main raw materials for sugar production. About 50% of the fiber of the bagasse, which remains after sugar pressing, can be used for paper making. However, some of the pith (marrow cells) have no interweaving force and should be removed before pulping. The bagasse fibers are about 0.65-2.17mm in length and 21-28 μm in width. Although the fiber form is inferior to wood and bamboo, the fiber form is better than rice and wheat straw fiber. Due to the reasons of site limitation, labor cost, transportation cost and the like, bagasse produced by most brown sugar manufacturers is only partially utilized, so that a large amount of resources are wasted.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a probiotic colony composition for degrading bagasse and a preparation method thereof, wherein the probiotic colony composition can fully decompose the bagasse, and the decomposed mixture can be used as high-quality biological feed or fermentation bed padding.
The technical scheme adopted by the invention is as follows:
a probiotic flora composition for degrading bagasse comprises the following raw material components:
probiotics, corn, bitter gourd, sugar cane and water;
the probiotic bacteria comprise yeast, Lactobacillus buchneri, Lactobacillus acidophilus, Bifidobacterium bifidum, enterococcus faecalis, Pediococcus acidilactici, Lactobacillus paracasei, Rhizopus nigricans and Aspergillus oryzae.
Furthermore, the weight ratio of the probiotics, the corn, the balsam pear, the sugarcane and the water in the raw material components is 0.4-0.6:1:1:1: 100.
Further, the weight ratio of yeast, lactobacillus buchneri, lactobacillus acidophilus, bifidobacterium bifidum, enterococcus faecalis, pediococcus acidilactici, lactobacillus paracasei, rhizopus nigricans and aspergillus oryzae in the probiotics is 3-5:3-5:3:2:2:2:1: 1.
The preparation method of the bagasse degrading probiotic flora composition comprises the following steps:
(1) cleaning corn, soaking in water, and filtering to obtain soaked corn for later use;
(2) cleaning bitter gourds and sugarcane, uniformly mixing the bitter gourds and the sugarcane with the soaked corns, and adding water to carry out pulping to obtain mixed pulp;
(3) sterilizing the mixed slurry to obtain sterilized mixed slurry; sterilizing the fermentation tank to obtain a sterilized fermentation tank; cooling the sterilized mixed slurry and the sterilized fermentation tank to obtain cooled mixed slurry and cooled fermentation tank for later use;
(4) and (3) taking part of the cooled mixed slurry, uniformly mixing the cooled mixed slurry with probiotics, transferring the cooled mixed slurry and the rest of the cooled mixed slurry into a cooled fermentation tank, uniformly stirring, and fermenting to obtain the bagasse-degrading probiotic flora composition.
Further, in the step (1), the soaking time is 3-5 h.
Further, in the step (3), the sterilization temperature of the mixed slurry and the sterilization temperature of the fermentation tank are both 110-120 ℃, the sterilization time of the mixed slurry is 20-30min, and the sterilization time of the fermentation tank is 15-25 min.
Further, in the step (3), the temperature of the cooled mixed slurry and the cooled fermentation tank is 35-40 ℃.
Further, in the step (4), the mass of the partially cooled mixed slurry accounts for 20-30% of the mass of the total cooled mixed slurry; the fermentation temperature is 35-40 ℃, and the fermentation time is 70-74 h.
A method for degrading bagasse by using the probiotic bacterial composition comprises the following steps:
a method for degrading bagasse using the probiotic bacterial composition: mixing the probiotic bacteria composition and bagasse according to a mass ratio of 1:10-100, adding water to adjust the humidity to 30% +/-5%, and performing closed fermentation at normal temperature for 10-15 days to complete the degradation of the bagasse.
The application of the probiotic composition for degrading the bagasse in preparation of biological feed.
The application of the bagasse degrading probiotic flora composition in preparing fermentation bed padding.
In addition, the probiotic flora composition can also be used for degradation treatment of straws, sawdust, corns, bran, bean pulp and the like.
The invention has the beneficial effects that:
the raw material components of the probiotic flora composition for degrading the bagasse comprise probiotics, corn, balsam pear, sugarcane and water, wherein the probiotics are prepared from saccharomycetes, lactobacillus buchneri, lactobacillus acidophilus, bacillus bifidus, enterococcus faecalis, pediococcus acidilactici, lactobacillus paracasei, rhizopus nigricans and aspergillus oryzae in a specific ratio. The yeast in the probiotic composition can ferment sugar into alcohol and carbon dioxide; the lactobacillus buchneri, lactobacillus acidophilus, bifidobacterium bifidum, enterococcus faecalis, pediococcus acidilactici and lactobacillus paracasei can effectively ferment and convert crude fibers such as pectin substances, lignin and the like and proteins rich in bagasse into absorbable small molecular substances; simultaneously, rhizopus nigricans and aspergillus oryzae are fermented to produce cellulase, cellulose in sugarcane bagasse is effectively degraded, and bagasse raw materials are effectively converted into microbial mycoprotein, bioactive small peptide amino acids, microbial active probiotics and a complex enzyme preparation to form an integrated biological fermentation feed or a fermentation bed padding through the degradation process of a series of probiotics. The probiotic flora composition can fully decompose bagasse, the degraded bagasse has consistent color and luster, no mildew, no caking or peculiar smell, and has special fermentation smell, the crude protein is more than or equal to 50 percent, the crude fiber is less than or equal to 8 percent, the crude ash is less than or equal to 8 percent, the lysine is more than or equal to 2.8 percent, the aflatoxin is less than or equal to 30.0mg/kg, the escherichia coli is less than or equal to 3000/100 g, and no salmonella is detected; the degraded mixture can be used as high-quality biological feed or fermentation bed padding. The preparation method is simple and easy to operate, low in cost and easy to popularize.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.
The following are the bacterial species sources and bacterial strain names involved in the examples of the present invention:
yeast: NRR01120 Beijing Huayue Biotech Co., Ltd
Lactobacillus buchneri: XG-J3326 Shanghai West Biotech Ltd
Lactobacillus acidophilus: 308084-36-8 Wuhan Jiangxin Yu Biotechnology GmbH
B, bifidobacterium bifidum: CICC 10395 Beijing Yuwei science and technology Co Ltd
Enterococcus faecalis: YB-29212 Shanghai Yubo Biotech Co., Ltd
Pediococcus acidilactici: JCM 8791 Shanghai Xinyu Biotech Co., Ltd
Lactobacillus paracasei: XG-J3714 Shanghai West Biotech Ltd
Rhizopus nigricans: CICC 41346 Beijing Yuwei science and technology Co Ltd
Aspergillus oryzae: HZ 11283 Shanghai Biotechnology Ltd
Example 1
The embodiment provides a probiotic flora composition for degrading bagasse, which comprises the following raw material components:
0.4kg of probiotics, 1kg of corn, 1kg of bitter gourd, 1kg of sugarcane and 100kg of water;
wherein the probiotics comprise 0.068kg of microzyme, 0.068kg of lactobacillus buchneri, 0.066kg of lactobacillus acidophilus, 0.044kg of bifidobacterium bifidum, 0.044kg of enterococcus faecalis, 0.044kg of pediococcus acidilactici, 0.022kg of lactobacillus paracasei, 0.022kg of rhizopus nigricans and 0.022kg of aspergillus oryzae.
The preparation method of the bagasse degrading probiotic flora composition comprises the following steps:
(1) cleaning corn, soaking in water for 3h, and filtering to obtain soaked corn for later use;
(2) cleaning bitter gourds and sugarcane, uniformly mixing the bitter gourds and the sugarcane with the soaked corns, and adding water to carry out pulping to obtain mixed pulp;
(3) sterilizing the mixed slurry at 110 ℃ for 30min to obtain sterilized mixed slurry; sterilizing the fermentation tank at 110 deg.C for 25min to obtain sterilized fermentation tank; cooling the sterilized mixed slurry and the sterilized fermentation tank to 35 ℃ to obtain cooled mixed slurry and cooled fermentation tank for later use;
(4) and (3) uniformly mixing the cooled mixed slurry accounting for 20% of the mass with the probiotics, transferring the mixed slurry together with the rest cooled mixed slurry into a cooled fermentation tank, uniformly stirring, and fermenting for 74 hours at 35 ℃ to obtain the bagasse degrading probiotic flora composition.
Mixing the probiotic colony composition and bagasse according to a mass ratio of 1:10, adding water to adjust the humidity to 25%, and performing closed fermentation at normal temperature for 10 days to complete the degradation of the bagasse; and then preparing the degraded bagasse mixture into biological feed.
Example 2
The embodiment provides a probiotic flora composition for degrading bagasse, which comprises the following raw material components:
0.6kg of probiotics, 1kg of corn, 1kg of bitter gourd, 1kg of sugarcane and 100kg of water;
wherein the probiotics comprise 0.108kg of yeast, 0.108kg of lactobacillus buchneri, 0.096kg of lactobacillus acidophilus, 0.064kg of bifidobacterium bifidum, 0.064kg of enterococcus faecalis, 0.064kg of pediococcus acidilactici, 0.032kg of lactobacillus paracasei, 0.032kg of rhizopus nigricans and 0.032kg of aspergillus oryzae.
The preparation method of the bagasse degrading probiotic flora composition comprises the following steps:
(1) cleaning corn, soaking in water for 5h, and filtering to obtain soaked corn for later use;
(2) cleaning bitter gourds and sugarcane, uniformly mixing the bitter gourds and the sugarcane with the soaked corns, adding water, and then grinding the mixture into thick liquid to obtain mixed thick liquid;
(3) sterilizing the mixed slurry at 120 ℃ for 20min to obtain sterilized mixed slurry; sterilizing the fermentation tank at 120 deg.C for 15min to obtain sterilized fermentation tank; cooling the sterilized mixed slurry and the sterilized fermentation tank to 40 ℃ to obtain cooled mixed slurry and cooled fermentation tank for later use;
(4) and (3) uniformly mixing the cooled mixed slurry accounting for 30% of the mass with probiotics, transferring the mixed slurry together with the rest cooled mixed slurry into a cooled fermentation tank, uniformly stirring, and fermenting at 40 ℃ for 70 hours to obtain the bagasse degrading probiotic flora composition.
Mixing the probiotic colony composition and bagasse according to a mass ratio of 1:100, adding water to adjust the humidity to 35%, and performing closed fermentation at normal temperature for 15 days to complete the degradation of the bagasse; and then preparing the degraded bagasse mixture into fermentation bed padding.
Example 3
The embodiment provides a probiotic flora composition for degrading bagasse, which comprises the following raw material components:
0.5kg of probiotics, 1kg of corn, 1kg of bitter gourd, 1kg of sugarcane and 100kg of water;
wherein the probiotics comprise 0.1kg of microzyme, 0.1kg of lactobacillus buchneri, 0.075kg of lactobacillus acidophilus, 0.05kg of bifidobacterium bifidum, 0.05kg of enterococcus faecalis, 0.05kg of pediococcus acidilactici, 0.025kg of lactobacillus paracasei, 0.025kg of rhizopus nigricans and 0.025kg of aspergillus oryzae.
The preparation method of the bagasse degrading probiotic flora composition comprises the following steps:
(1) cleaning corn, adding water, soaking for 4h, and filtering to obtain soaked corn for later use;
(2) cleaning bitter gourds and sugarcane, uniformly mixing the bitter gourds and the sugarcane with the soaked corns, adding water, and then grinding the mixture into thick liquid to obtain mixed thick liquid;
(3) sterilizing the mixed slurry at 115 ℃ for 25min to obtain sterilized mixed slurry; sterilizing the fermentation tank at 115 deg.C for 20min to obtain sterilized fermentation tank; cooling the sterilized mixed slurry and the sterilized fermentation tank to 38 ℃ to obtain cooled mixed slurry and cooled fermentation tank for later use;
(4) and (3) uniformly mixing the cooled mixed slurry accounting for 25% by mass with probiotics, transferring the mixed slurry together with the rest cooled mixed slurry into a cooled fermentation tank, uniformly stirring, and fermenting at 38 ℃ for 72 hours to obtain the bagasse degrading probiotic flora composition.
Mixing the probiotic bacteria composition with bagasse according to a mass ratio of 1:55, adding water to adjust the humidity to 30% to 5, and performing closed fermentation at normal temperature for 13 days to complete the degradation of the bagasse.
Examples of the experiments
The results of the tests carried out on bagasse degraded with the composition described in example 3 are shown in Table 1.
TABLE 1 bagasse indices after degradation
As can be seen from the table data, the bagasse treated by the method in the embodiment 3 of the invention meets the standard requirements in all indexes, can effectively degrade the bagasse, can be further prepared into biological feed or fermentation bed padding, and is suitable for large-scale popularization, production and use.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.
Claims (10)
1. The probiotic flora composition for degrading bagasse is characterized by comprising the following raw material components:
probiotics, corn, bitter gourd, sugar cane and water;
the probiotic bacteria comprise yeast, Lactobacillus buchneri, Lactobacillus acidophilus, Bifidobacterium bifidum, enterococcus faecalis, Pediococcus acidilactici, Lactobacillus paracasei, Rhizopus nigricans and Aspergillus oryzae.
2. A bagasse-degrading probiotic flora composition according to claim 1, characterized in that the weight ratio of the probiotics, corn, bitter gourd, sugarcane and water in the raw material components is 0.4-0.6:1:1:1: 100.
3. The bagasse-degrading probiotic bacterial population composition according to claim 1, wherein the weight ratio of yeast, lactobacillus buchneri, lactobacillus acidophilus, bifidobacterium bifidum, enterococcus faecalis, pediococcus acidilactici, lactobacillus paracasei, rhizopus nigricans and aspergillus oryzae in the probiotic bacteria is 3-5:3-5:3:2:2:2:1:1: 1.
4. A process for the preparation of a bagasse-degrading probiotic flora composition according to any one of claims 1-3, characterized in that it comprises the following steps:
(1) cleaning corn, soaking in water, and filtering to obtain soaked corn for later use;
(2) cleaning bitter gourds and sugarcane, uniformly mixing the bitter gourds and the sugarcane with the soaked corns, and adding water to carry out pulping to obtain mixed pulp;
(3) sterilizing the mixed slurry to obtain sterilized mixed slurry; sterilizing the fermentation tank to obtain a sterilized fermentation tank; cooling the sterilized mixed slurry and the sterilized fermentation tank to obtain cooled mixed slurry and cooled fermentation tank for later use;
(4) and (3) taking part of the cooled mixed slurry, uniformly mixing the cooled mixed slurry with probiotics, transferring the cooled mixed slurry and the rest of the cooled mixed slurry into a cooled fermentation tank, uniformly stirring, and fermenting to obtain the bagasse-degrading probiotic flora composition.
5. The process for preparing a probiotic flora composition for degrading bagasse according to claim 4, characterized in that in step (1), the soaking time is 3-5 h.
6. The method for preparing a bagasse-degrading probiotic flora composition according to claim 4, wherein in step (3), the sterilization temperature of the mixed slurry and the sterilization time of the fermentation tank are both 110-120 ℃, the sterilization time of the mixed slurry is 20-30min, and the sterilization time of the fermentation tank is 15-25 min.
7. The method for preparing a bagasse-degrading probiotic flora composition according to claim 4, wherein in the step (3), the temperature of the cooled mixed pulp and the cooled fermentation tank is 35-40 ℃.
8. The method for preparing a bagasse-degrading probiotic flora composition according to claim 4, wherein in the step (4), the partially cooled mixed pulp accounts for 20-30% by mass of the total cooled mixed pulp; the fermentation temperature is 35-40 ℃, and the fermentation time is 70-74 h.
9. A method for degrading sugar cane bagasse using a probiotic bacterial composition according to any one of claims 1-3, characterized by comprising the following steps:
mixing the probiotic bacteria composition and bagasse according to a mass ratio of 1:10-100, adding water to adjust the humidity to 30% +/-5%, and performing closed fermentation at normal temperature for 10-15 days to complete the degradation of the bagasse.
10. Use of a bagasse-degrading probiotic bacterial composition according to any one of claims 1-3 in the preparation of a biological feed or a fermentation bed material.
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| CN114158485A (en) * | 2021-12-03 | 2022-03-11 | 安徽省农业科学院农业工程研究所 | Zero-emission straw padding for pig breeding and preparation method thereof |
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| CN114158485A (en) * | 2021-12-03 | 2022-03-11 | 安徽省农业科学院农业工程研究所 | Zero-emission straw padding for pig breeding and preparation method thereof |
| CN114158485B (en) * | 2021-12-03 | 2022-12-27 | 安徽省农业科学院农业工程研究所 | Zero-emission straw padding for pig breeding and preparation method thereof |
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