CN111632201B - Application of nanofiber felt cloth in preparation of bone defect repair product or osteogenesis promoting product - Google Patents
Application of nanofiber felt cloth in preparation of bone defect repair product or osteogenesis promoting product Download PDFInfo
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- CN111632201B CN111632201B CN202010634366.XA CN202010634366A CN111632201B CN 111632201 B CN111632201 B CN 111632201B CN 202010634366 A CN202010634366 A CN 202010634366A CN 111632201 B CN111632201 B CN 111632201B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/16—Macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
Abstract
The invention provides application of nanofiber felt cloth in preparing bone defect repair products or osteogenesis promoting products. The nano-fiber felt cloth can effectively collect the biological mechanical energy and convert the biological mechanical energy into electric energy, is directly used for electrical stimulation of cells at the bone defect part, and realizes self-driven electrical stimulation osteogenesis of a host while needing no external energy supply system and complex circuit.
Description
Technical Field
The invention relates to the field of non-woven fabrics, in particular to application of nano-fiber felt cloth in preparing bone defect repair products or bone formation promoting products.
Background
Bones are important components of the human body, have functions of protecting, providing exercise support, storing minerals and the like, and provide blood cells for the human circulatory system and the immune system. Bone defects caused by trauma, inflammation, tumor after operation and the like can cause partial form and function loss, and bring serious pain to patients. To date, clinical bone defects are in enormous cases and present an increasing trend year by year. The repair of bone defects is one of the problems to be solved clinically at present, especially in the field of repair of large-size, difficult-to-heal and pathological defects.
Bone grafting is the main method for clinically treating and repairing bone defects at present, and comprises autologous bone grafting and allogeneic bone grafting, but both have disadvantages. The autologous bone transplantation needs to open a second operation area, and has limited supply amount, thus easily causing complications such as chronic pain, infection and the like. The bone creeping replacement of the allogeneic bone transplantation is slow, collapse and fracture often occur at the late stage, and potential risks of disease transmission exist, so that the allograft bone transplantation is limited. The bone repair strategy taking exogenous electrical stimulation as a main body has important prospect in the aspect of clinical treatment and bone defect repair application. Compared with traditional chemical stimulation and drug therapy, the electrical stimulation method has the advantages of mild conditions, avoidance of chemical injury and drug side effects of organisms and the like. However, in the field of electrical stimulation regulation of bone tissue repair and regeneration in vivo, there are problems in that the limitation of an external energy supply system is broken, and the resistance to the impact of biomechanical energy is enhanced. Therefore, the development of a class of bone repair materials and devices capable of stimulating cell growth in situ and regulating osteogenic differentiation is one of the key paths for solving the bottleneck of clinical application.
Disclosure of Invention
In view of the above-mentioned drawbacks of the prior art, the present invention aims to provide a use of a nanofiber felt for preparing a bone defect repair product or an osteogenesis promoting product, for solving the technical problem of bone defect repair in the prior art.
To achieve the above objects and other related objects, the present invention includes the following technical solutions.
The invention provides application of nanofiber felt cloth in preparing bone defect repair products or osteogenesis promoting products.
According to the use, the nanofiber felt is a non-woven fabric composed of hollow nanofibers formed from a biodegradable polymer, and the nanofibers in the nanofiber felt have an orientation.
According to the application, the gram weight of the nanofiber felt is (1-15) g/m2。
According to the application, the nanofiber felt is prepared by adopting the following method: taking an organic solvent solution of a biodegradable polymer as a skin layer spinning solution, carrying out coaxial electrostatic spinning with a core layer spinning solution to obtain felt cloth, and dissolving the felt cloth in a core layer solvent or heating to remove a core layer material.
According to the above-mentioned use, the biodegradable polymer is selected from one or more of polycaprolactone (abbreviated as PCL), polylactic-co-glycolic acid (abbreviated as PLGA), levopolylactic acid (abbreviated as PLLA), polyvinylpyrrolidone (abbreviated as PVP), poly (3-hydroxy-3-valerate) (abbreviated as PHBV), polyvinyl alcohol (abbreviated as PVA) and chitosan. Preferably, the number average molecular weight of the biodegradable polymer is 20000-200000.
According to the above-mentioned use, the organic solvent is selected from one or more of chloroform, dichloromethane, hexafluoroisopropanol, trifluoroacetic acid, dimethylformamide (abbreviated as DMF), acetone and dimethylsulfoxide (abbreviated as DMSO).
According to the application, the concentration of the skin layer spinning solution is (6-20) wt%.
The use as described above, wherein the core layer spinning solution is one or more selected from the group consisting of water, liquid paraffin and mineral oil.
The coaxial electrostatic spinning technology in the application is as follows: the sheath spinning solution and the core layer spinning solution are respectively arranged in two different injectors, a spinning system consists of two coaxial capillaries with different inner diameters, the sheath spinning solution and the core layer spinning solution are converged under the action of a high-voltage electric field, and after the sheath spinning solution and the core layer spinning solution are stretched and received by a receiving device roller, a sheath-core structure fiber tow is formed and wound on the surface of the roller to form felt cloth, a core layer material of the felt cloth is dissolved and removed through a core layer solvent, a sheath layer material is left, and then the nano-fiber felt cloth formed by hollow fibers is obtained.
According to the application, in the coaxial electrostatic spinning, the rotating speed of a roller is 500-5000 rpm.
According to the application, in the coaxial electrostatic spinning, the ejection speed of the skin layer spinning solution is 0.1-3.0 mL/h.
According to the application, in the coaxial electrostatic spinning, the ejection speed of the core layer spinning solution is 10-75% of that of the skin layer spinning solution.
According to the application, in the coaxial electrostatic spinning, the receiving distance is 3-30 cm. The common receiving distance in this application refers to the distance between the outlet of the spinneret of the spinning system to the central axis of the drum of the receiving device.
According to the application, in the coaxial electrostatic spinning, a direct-current voltage of 10-50 KV is applied.
According to the application, in the coaxial electrostatic spinning, the environment temperature is 23-27 ℃.
According to the application, in the coaxial electrostatic spinning, the environment humidity is 20-30%.
According to the application, the solvent is mutually soluble with the core layer spinning solution and is not mutually soluble with the organic solvent in the skin layer spinning solution. Preferably, the core layer solvent is selected from one or more of cyclohexane, benzene, toluene and xylene.
The application can specifically be as follows: sterilizing the nanofiber felt cloth, cutting the nanofiber felt cloth into a size matched with the bone defect position, covering the surface of the bone defect position, keeping the orientation direction of the fibers basically consistent with the stretching direction of peripheral muscles, and sewing the nanofiber felt cloth at two ends of the muscles to ensure that the nanofibers can be stretched along the orientation direction when the muscles contract, thereby realizing the compression of the porous structure in the nanofibers. The sterilization treatment mode is as follows: soaking in 75% ethanol while irradiating with ultraviolet lamp. The treatment time is at least 2 h.
When the nanofiber felt is stretched by muscle orientation, the porous structure inside the nanofibers is compressed, the induction distance of the internal electret charges is shortened, the induced charges on the surfaces of the fibers are migrated, and therefore, a potential difference is generated between the upper surface and the lower surface of the nanofiber felt and a current is generated until new charge balance is achieved. When the muscle is recovered to a relaxed state, the compressed state of the porous structure in the nano fiber disappears, the internal electret charge induction distance is recovered, and the induced charges on the surface of the fiber generate reverse migration, so that reverse potential differences are generated on the upper surface and the lower surface of the nano fiber felt cloth, and reverse current is generated until new charge balance is achieved. The whole process is the process of collecting the mechanical energy generated by one muscle contraction and converting the mechanical energy into electric energy by the nano-fiber felt cloth.
The nano-fiber felt cloth collects the biomechanical energy of muscles and converts the biomechanical energy into electric energy, and is used for electrically stimulating cells at the bone defect part, particularly stem cells, to differentiate towards the osteogenic direction in situ, so as to realize the rapid and accurate repair of the bone defect.
The technical scheme of the invention has the beneficial effects that:
the technical scheme of the invention is that the nanofiber with an internal porous structure is constructed by a coaxial electrostatic spinning technology, and the electrical stimulation nanofiber felt cloth capable of promoting osteogenesis is obtained by orientation arrangement of the nanofiber. The nano-fiber felt cloth can effectively collect the biological mechanical energy and convert the biological mechanical energy into electric energy, is directly used for electrical stimulation of cells at a bone defect part, particularly stem cells, and realizes self-driven electrical stimulation osteogenesis of a host while an external energy supply system and a complex circuit are not needed.
Drawings
FIG. 1 is a micro-CT image of a blank group, a control group and an experimental group in example 1 of the present application
FIG. 2 is a bar graph showing data obtained after quantitative analysis of the micro-CT image of FIG. 1
Detailed Description
The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will become apparent to those skilled in the art from the present disclosure.
Before the present embodiments are further described, it is to be understood that the scope of the invention is not limited to the particular embodiments described below; it is also to be understood that the terminology used in the examples is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention. Test methods in which specific conditions are not specified in the following examples are generally carried out under conventional conditions or under conditions recommended by the respective manufacturers.
When numerical ranges are given in the examples, it is understood that both endpoints of each of the numerical ranges and any value therebetween can be selected unless the invention otherwise indicated. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In addition to the specific methods, devices, and materials used in the examples, any methods, devices, and materials similar or equivalent to those described in the examples may be used in the practice of the invention in addition to the specific methods, devices, and materials used in the examples, in keeping with the knowledge of one skilled in the art and with the description of the invention.
Example 1
The preparation method of the nanofiber felt cloth in this embodiment is specifically as follows:
(1) preparing a hexafluoroisopropanol solution of polycaprolactone with the concentration of 10 wt% as a skin layer spinning solution for coaxial electrospinning, wherein the core layer spinning solution is liquid paraffin; the number average molecular weight of the polycaprolactone is 80000;
(2) a syringe needle with the specification of G17/G22 is selected and coaxially arranged at the positive end of a high-voltage generator, the negative end of the syringe needle is a roller driven by a motor, the rotating speed of the roller is 800rpm, and the roller is in a grounding state. Respectively placing the prepared skin layer spinning solution and core layer spinning solution into a cylinder of a propulsion pump, wherein the propulsion speed of the skin layer spinning solution is 1.0mL/h, the propulsion speed of the core layer spinning solution is 30% of the skin layer speed, the receiving distance is 15cm, the voltage is 20KV, after spinning is finished, taking down the felt cloth, placing the felt cloth into cyclohexane for 24 hours to remove core layer materials, and drying at room temperature to obtain the nanofiber felt cloth, wherein the gram weight of the nanofiber felt cloth in the embodiment is 8.0g/m2。
The nanofiber felt cloth obtained in the embodiment is implanted into a bone defect to be repaired, and the specific operation is as follows:
sterilizing the obtained nanofiber felt, cutting into a rectangle with the diameter of 6 x 15mm, enabling the short side of the rectangle to be consistent with the orientation direction, constructing a circular defect with the diameter of 3mm on the single side of the middle section of the rat femur, covering the nanofiber felt on the surface of the defect, keeping the fiber orientation direction to be basically consistent with the peripheral muscle stretching direction, and sewing the nanofiber felt at two ends of the muscle, so that the nanofibers can be stretched along the orientation direction when the muscle is contracted, and further compression of a porous structure in the nanofibers is realized.
The sterilization treatment mode is as follows: soaking in 75% ethanol while irradiating with ultraviolet lamp. The treatment time was 2 h.
The nanofiber felt with the self-driven electrical stimulation function in the embodiment is subjected to in vivo electrical property characterization.
Control group: the control was a solid oriented nanofiber felt prepared according to the same preparation method as in the example, except that: the advancing speed of the core layer spinning solution is zero. Thereby forming a solid oriented nanofiber mat.
After the nanofiber felt cloth formed in the example and the solid oriented nanofiber felt cloth formed in the control group were implanted into the rat body, the lead was led out to form a closed loop, and the open-circuit voltage and the short-circuit current of the rat during each hind leg movement were detected using an oscilloscope. The nanofiber mats in this example had an open circuit voltage of 5V and a short circuit current of 20nA, while the control solid oriented nanofiber mats had no significant power output. Therefore, it can be obviously obtained that the hollow nanofiber felt cloth in the embodiment has the functions of mechanical energy collection and electric energy conversion, when the hollow nanofiber felt cloth is used for bone defect repair, the electric stimulation function can be realized according to muscle movement, and the solid oriented nanofiber felt cloth of the control group does not have the functions of mechanical energy collection and electric energy conversion.
The rat model in this example is: adult SD rats, body weight 200 ~ 250 g, male.
Rats were randomly divided into 6 groups, each of which was 3 in number, and each group was assigned to a blank group, a control group and an experimental group, and a unilateral 3mm circular defect was constructed in the middle part of the right femur of each rat in each group.
The nanofiber felt cloth in this example was subjected to an in vivo osteogenesis experiment, and the solid oriented nanofiber felt cloth was used as a control group, and the bone defect was directly sutured without covering any membrane, and used as a blank group. The materials were obtained at 4 weeks and 8 weeks, respectively, and the results of the experiment are shown in table 1, fig. 1 and fig. 2.
TABLE 1
As is apparent from the results of fig. 1 and 2, the experimental group using the nanofiber felt having the self-driven electrical stimulation function of example 1 of the present application had a higher new bone mass at two time points, while the blank group had a very limited new bone mass, from which it was apparent that the osteogenesis was significantly promoted using this hollow oriented nanofiber felt of the present application.
In this example, the new bone mass was tested by micro-CT.
Example 2
The preparation method of the nanofiber felt cloth in this embodiment is specifically as follows:
(1) preparing a chloroform solution of 10 wt% polylactic acid-glycolic acid copolymer as a skin layer spinning solution for coaxial electrospinning, wherein the core layer spinning solution is deionized water; the number average molecular weight of the polylactic acid-glycolic acid copolymer is 300000;
(2) a syringe needle with the specification of G17/G22 is selected and coaxially arranged at the positive end of a high-voltage generator, the negative end of the syringe needle is a roller driven by a motor, the rotating speed of the roller is 800rpm, and the roller is in a grounding state. Respectively placing the prepared skin layer spinning solution and core layer spinning solution into a cylinder of a propulsion pump, wherein the propulsion speed of the skin layer spinning solution is 0.8mL/h, the propulsion speed of the core layer spinning solution is 30% of the skin layer speed, the receiving distance is 15cm, the voltage is 18KV, and after spinning is finished, drying and removing the core layer material at room temperature to obtain the hollow oriented nano fiber felt cloth, wherein the gram weight of the nano fiber felt cloth is 12g/m2。
The nanofiber felt with the self-driven electrical stimulation function and the control group in the embodiment are subjected to in vivo electrical property characterization.
The control was a solid oriented nanofiber felt prepared according to the same preparation method as in the example, except that: the advancing speed of the core layer spinning solution is zero. Thereby forming a solid oriented nanofiber mat.
After the nanofiber felt cloth formed in the embodiment and the control group material are respectively implanted into a rat body, a lead is led out to form a closed loop, and an oscilloscope is used for detecting open-circuit voltage and short-circuit current of the rat during each hind leg movement. The nanofiber mats in this example had an open circuit voltage of 3.5V and a short circuit current of 12nA, while the control solid oriented nanofiber mats had no significant power output. Therefore, it can be obviously obtained that the hollow nanofiber felt cloth in the embodiment has the functions of mechanical energy collection and electric energy conversion, when the hollow nanofiber felt cloth is used for bone defect repair, the electric stimulation function can be realized according to muscle movement, and the solid oriented nanofiber felt cloth of the control group does not have the functions of mechanical energy collection and electric energy conversion.
Example 3
The preparation method of the nanofiber felt cloth in this embodiment is specifically as follows:
(1) preparing hexafluoroisopropanol solution of the levorotatory polylactic acid with the concentration of 10 wt% as the skin layer spinning solution of coaxial electrospinning, wherein the core layer spinning solution is mineral oil; the number average molecular weight of the levorotatory polylactic acid is 70000.
(2) A syringe needle with the specification of G17/G22 is selected and coaxially arranged at the positive end of a high-voltage generator, the negative end of the syringe needle is a roller driven by a motor, the rotating speed of the roller is 600rpm, and the roller is in a grounding state. Respectively placing the prepared skin layer spinning solution and core layer spinning solution into a cylinder of a propulsion pump, wherein the propulsion speed of the skin layer spinning solution is 1.0mL/h, the propulsion speed of the core layer spinning solution is 20% of the skin layer speed, the receiving distance is 15cm, the direct current voltage is 20KV, after spinning is finished, taking down the felt cloth, placing the felt cloth in petroleum ether for 24 hours, removing a core layer material, and drying at room temperature to obtain the composite spinning felt clothA nanofiber felt; the gram weight of the nanofiber felt in this example was 15g/m2。
The nanofiber felt with the self-driven electrical stimulation function and the control group in the embodiment are subjected to in vivo electrical property characterization.
The control was a solid oriented nanofiber felt prepared according to the same preparation method as in the example, except that: the advancing speed of the core layer spinning solution is zero. Thereby forming the solid oriented nano-fiber felt
After the nanofiber felt cloth formed in the embodiment and the control group material are respectively implanted into a rat body, a lead is led out to form a closed loop, and an oscilloscope is used for detecting open-circuit voltage and short-circuit current of the rat during each hind leg movement. The nanofiber mats in this example had an open circuit voltage of 2V and a short circuit current of 7nA, while the control solid oriented nanofiber mats had no significant power output. Therefore, it can be obviously obtained that the hollow nanofiber felt cloth in the embodiment has the functions of mechanical energy collection and electric energy conversion, when the hollow nanofiber felt cloth is used for bone defect repair, the electric stimulation function can be realized according to muscle movement, and the solid oriented nanofiber felt cloth of the control group does not have the functions of mechanical energy collection and electric energy conversion.
The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.
Claims (9)
1. The application of the nano-fiber felt cloth in preparing bone defect repair products or osteogenesis promoting products;
the nanofiber felt is a non-woven fabric formed by hollow nanofibers formed by biodegradable polymers, and the nanofibers in the nanofiber felt have orientation.
2. Use according to claim 1, wherein the nanofiber felt has a grammage of (1-15) g/m2。
3. The use according to any one of claims 1 to 2, wherein the nanofiber felt is prepared by a method comprising: taking an organic solvent solution of a biodegradable polymer as a skin layer spinning solution, carrying out coaxial electrostatic spinning with a core layer spinning solution to obtain felt cloth, and dissolving the felt cloth in a core layer solvent or heating to remove a core layer material.
4. Use according to claim 3, wherein the biodegradable polymer is selected from one or more of polycaprolactone, polylactic acid-co-glycolic acid, L-polylactic acid, polyvinylpyrrolidone, poly (3-hydroxy-3-valerate), polyvinyl alcohol and chitosan.
5. Use according to claim 3, wherein the organic solvent is selected from one or more of chloroform, dichloromethane, hexafluoroisopropanol, trifluoroacetic acid, dimethylformamide, acetone and dimethyl sulfoxide.
6. Use according to claim 3, wherein the concentration of the sheath dope is (6-20) wt%.
7. Use according to claim 3, wherein the core dope is one or more selected from water, liquid paraffin and mineral oil.
8. Use according to claim 3, characterized in that in said coaxial electrospinning, one or more of the following features are included:
the rotating speed of the roller is 500-5000 rpm;
the ejection speed of the skin layer spinning solution is 0.1-3.0 mL/h;
the ejection speed of the core layer spinning solution is 10-75% of that of the skin layer spinning solution;
the receiving distance is 3-30 cm;
applying a direct-current voltage of 10-50 KV;
the ambient temperature is 23-27 ℃;
the environmental humidity is 20-30%.
9. Use according to claim 3, wherein the core layer solvent is selected from one or more of cyclohexane, benzene, toluene, xylene and petroleum ether.
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| CN103061037A (en) * | 2013-01-11 | 2013-04-24 | 东华大学 | Method for manufacturing polyaspartic acid nano-fiber mat by electrostatic spinning |
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| US10238772B2 (en) * | 2013-03-15 | 2019-03-26 | The Curators Of The University Of Missouri | Biodegradable composite scaffold for repairing defects in load-bearing bones |
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| CN103061037A (en) * | 2013-01-11 | 2013-04-24 | 东华大学 | Method for manufacturing polyaspartic acid nano-fiber mat by electrostatic spinning |
| CN104032423A (en) * | 2014-06-20 | 2014-09-10 | 东华大学 | Nano-fiber covering yarn electrostatic spinning device and application thereof |
| CN104774762A (en) * | 2015-03-31 | 2015-07-15 | 苏州睿研纳米医学科技有限公司 | Oriented polymer nanometer fibrocyte culture plate and preparation method thereof |
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