CN105455923A - Neural restoration sleeve tube and preparation method and application thereof - Google Patents
Neural restoration sleeve tube and preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/26—Mixtures of macromolecular compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/04—Macromolecular materials
- A61L31/041—Mixtures of macromolecular compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/04—Macromolecular materials
- A61L31/06—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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Abstract
The invention provides a neural restoration sleeve tube and a preparation method and application thereof. The neural restoration sleeve tube is a double-layer fiber sleeve tube, the inner layer of the sleeve tube is an orientated polymer fiber layer, the outer layer of the sleeve tube is a non-woven non-orientated polymer fiber layer, and the polymer is a bioresorbable polymer. According to the neural restoration sleeve tube prepared through the preparation method, nerve cells can grow along the fiber orientation, then nervous tissue is restored, the non-woven non-non-orientated polymer fiber layer on the outer layer achieves a supporting function, and the neural restoration sleeve tube achieves good strength and stability. Meanwhile, due to the fact that the polymer on the inner layer and the outer layer is the bioresorbable polymer, good biocompatibility is achieved, no chronic inflammation reaction is caused, high safety is achieved, the preparation method is simple, and industrial production can be achieved.
Description
Technical field
The invention belongs to nerve cuff Manifold technology field, relate to a kind of CO2 laser weld sleeve pipe and its preparation method and application.
Background technology
It is the disease that often can run in clinical position that peripheral nerve sustains damage, and can cause forfeiture and the dyskinesia of sensation.Because neurocyte does not divide, its growth does not have penetration power, makes its regeneration in vivo very difficult.Current employing tissue or cell transplantation, tissue and the various Intervention Strategy such as the nerve stimulation of the physical factor such as biological engineering, electromagnetism or regulation and control, medicine or chemistry, in original neuro anatomy and function basis, promote destroyed or undermined neuranagenesis reparation and reinvent, rebuild neuro anatomy projection tract and loop, regulation and control and improve Neurotransmission, all part finally can realize function of nervous system and repair.
Nerve cuff Manifold technology, because can avoid sacrificing autologous nerve, causes nerve damage and be subject to extensive concern when can avoid identical simultaneously.Neural sleeve pipe needs to play the effect supporting neural axon migration, and shell material not only needs to have good safety and biocompatibility, and also requirement can be beneficial to operation simultaneously, promotes nerve growth.The natural material that can be used as neural sleeve pipe comprises blood vessel, degeneration muscle, laminin, FN, collagen protein etc., natural material causes disadvantageous immunoreation sometimes, although immunosuppressant can play a role, then not easily use under damage or tumor presence; In addition, native protein is also difficult to batch production, and quality and performance are all difficult to control, and more sight is turned to artificial material by people.Nondegradable artificial material sleeve pipe comprises silica gel sleeve pipe and teflon sleeve, this type of sleeve pipe of simple use also regenerates without facilitation neural axon, and human body can be retained in foreign body form after this type of sleeve pipe implant into body, easily cause chronic inflammatory reaction, excessive scar tissue formation can cause nerve compression, needs second operation to be taken out by sleeve pipe time serious again.Adsorbable artificial material is well biological, and part degradation material can promote that neurocyte better grows, but its induction behavior does not possess directivity, and intensity and shape not easily long term maintenance after implanting, make its Clinical practice very limited.
Electrostatic spinning is a kind of simple and effective processing technique preparing polymer superfine fibre, and the application that electrostatic spinning goods are particularly suitable in biomedical material owing to having the feature such as extra specific surface area and super large porosity.
Therefore, expect that can obtain one has good biocompatibility in the art, and there is single induced orientation, and there is the CO2 laser weld sleeve pipe of good mechanical strength and stability.
Summary of the invention
For the deficiencies in the prior art, the object of the present invention is to provide a kind of CO2 laser weld sleeve pipe and its preparation method and application.
For reaching this object, the present invention by the following technical solutions:
First aspect, the invention provides a kind of CO2 laser weld sleeve pipe, and described CO2 laser weld sleeve pipe is double layer fibre sleeve pipe, and the internal layer of described sleeve pipe is orientated polymer fibrous layer, and skin is nonwoven No yield point polymer fibres layers, and described polymer is biologically absorbable polymer.
CO2 laser weld cover inner tube layer of the present invention is orientated polymer fibrous layer, it can make neuron grow along fibre orientation, and outer field nonwoven No yield point polymer fibres layers is played a supporting role, and give CO2 laser weld sleeve pipe of the present invention with good strength and stability, simultaneously because the polymer of ectonexine is biologically absorbable polymer, it has good biocompatibility, and this sleeve pipe implant into body can not be caused chronic inflammatory reaction, safe and reliable.
In CO2 laser weld sleeve pipe of the present invention, described polymer is the combination of any one or at least two kinds in polycaprolactone, polylactic acid, glycolide-lactide copolymer, caprolactone-glycolide copolymer, caprolactone-carbonate copolymer or Ju diethyleno dioxide ketone.
Preferably, the relative molecular weight of described polymer is 60,000-200 ten thousand, such as 6.5 ten thousand, 70,000,80,000,90,000,100,000,200,000,300,000,400,000,500,000,700,000,900,000,1,000,000,1,200,000,1,400,000,1,600,000,1,800,000,1,900,000.
Preferably, described glycolide-lactide copolymer is the glycolide-lactide copolymer that glycolide content is greater than 80%.
Preferably, described caprolactone-glycolide copolymer is caprolactone-glycolide copolymer that caprolactone content is greater than 70%.
Preferably, described caprolactone-carbonate copolymer is caprolactone-carbonate copolymer that caprolactone content is greater than 70%.
In CO2 laser weld sleeve pipe of the present invention, the diameter of described polymer fiber is 100 ~ 5000nm, such as 110nm, 120nm, 140nm, 160nm, 180nm, 200nm, 300nm, 400nm, 500nm, 600nm, 800nm, 1000nm, 1300nm, 1500nm, 1800nm, 2000nm, 2500nm, 3000nm, 3400nm, 3800nm, 4000nm, 4500nm, 4800nm or 5000nm, preferably 100 ~ 1000nm, further preferred 100 ~ 500nm.
Preferably, the thickness of described internal layer is 40 ~ 100 μm, such as 45 μm, 48 μm, 50 μm, 53 μm, 55 μm, 58 μm, 60 μm, 65 μm, 68 μm, 70 μm, 75 μm, 78 μm, 80 μm, 83 μm, 85 μm, 88 μm, 90 μm, 95 μm or 99 μm, preferably 50 ~ 100 μm.
Preferably, described outer layer thickness is 20 ~ 60 μm, such as 23 μm, 25 μm, 28 μm, 30 μm, 33 μm, 35 μm, 38 μm, 40 μm, 45 μm, 48 μm, 50 μm, 53 μm, 55 μm, 58 μm or 60 μm.
Preferably, described internal layer thickness is 1.5-3 times of outer layer thickness, such as 1.5 times, 1.7 times, 1.9 times, 2 times, 2.2 times, 2.3 times, 2.4 times, 2.5 times, 2.6 times, 2.7 times, 2.8 times, 2.9 times or 3 times, is preferably 2 times.
In the present invention, when internal layer thickness is more than or equal to 50 μm, or internal layer thickness is 1.5-3 times of outer layer thickness, when being particularly preferably 2 times, neural axon cell prolongs machine direction growth in described CO2 laser weld sleeve surface growth with having orientation, thus be suitable as peripheral nervous repairing casing, when after neural ankylose, this sleeve pipe can automatic degraded and absorbed.
On the other hand, the invention provides the preparation method of CO2 laser weld sleeve pipe as described in relation to the first aspect, described method is: polymer solution is injected electrospinning device and carry out spinning, the fiber formed penetrates from the spinning head of electrospinning device, regulate receiving system to obtain internal layer orientation fibers layer and outer nonwoven No yield point fibrous layer respectively, namely obtain described CO2 laser weld sleeve pipe.
In preparation method of the present invention, the preparation method of described polymer solution is: polymer is dissolved in organic solvent that to be mixed with mass body volume concentrations be 1-30% (such as 1%, 2%, 5%, 8%, 10%, 12%, 15%, 18%, 20%, 22%, 24%, 26%, 28% or 30%) solution, then at 50-80 DEG C (such as 52 DEG C, 55 DEG C, 57 DEG C, 59 DEG C, 60 DEG C, 62 DEG C, 64 DEG C, 66 DEG C, 68 DEG C, 70 DEG C, 72 DEG C, 74 DEG C, 76 DEG C, 78 DEG C or 79 DEG C) stir 12-24h (such as 13h, 14h, 15h, 17h, 19h, 20h, 21h, 22h or 23h) obtain described polymer solution.
Preferably, described organic solvent is the mixed liquor of hexafluoroisopropanol or chloroform and DMF.
Preferably, described chloroform and N, in the mixed liquor of dinethylformamide, the volume ratio of chloroform and DMF is 1:1-1:3, such as 1:1,1:1.2,1:1.4,1:1.6,1:1.8,1:2,1:2.2,1:2.4,1:2.6,1:2.8 or 1:3.
In the present invention, when polymer is for poly-diethyleno dioxide ketone, organic solvent used can be hexafluoroisopropanol, when polymer is other polymer except poly-diethyleno dioxide ketone of the present invention, organic solvent used can be the mixed liquor of chloroform and DMF.
In preparation method of the present invention, optimum configurations when described electrospinning device carries out spinning is: spinning head voltage is 10 ~ 30kV, such as 12kV, 14kV, 16kV, 18kV, 20kV, 22kV, 24kV, 26kV, 28kV or 29kV, solution flow rate is 1 ~ 5mL/h, such as 1.2mL/h, 1.4mL/h, 1.6mL/h, 1.8mL/h, 2mL/h, 2.3mL/h, 2.5mL/h, 2.8mL/h, 3mL/h, 3.3mL/h, 3.5mL/h, 3.8mL/h, 4mL/h, 4.4mL/h, 4.8mL/h or 4.9mL/h.
Preferably, the receiving range between described receiving system and spinning head is 5 ~ 25cm, such as 6cm, 8cm, 10cm, 12cm, 15cm, 17cm, 19cm, 22cm or 24cm.
In preparation method of the present invention, receiving system in electrospinning device is transformed, bipolar electrode is prepared as collecting electrode in described receiving system, as shown in Figure 1, the axis of bipolar electrode is in parallel arranged on same level line, and in described bipolar electrode, the diameter of each electrode is 2 ~ 20mm, and bipolar electrode spacing is 1 ~ 10cm, synchronous rotating Vortex, electrode can rotate through belt transmission.
In the present invention, carried out the preparation of superfine fibre by electrostatic spinning process, by changing the voltage that electrode is connected, thus make fiber produce orientation or nonwoven No yield point fiber, the sleeve pipe finally obtained sterilizing after evacuation can use.
Preferably, described adjustment receiving system obtains internal layer orientation fibers layer respectively and the fibrolaminar method of outer nonwoven No yield point is: first by one of bipolar electrode ground connection in receiving system, another electrode meets 0.5 ~ 3kV (such as 0.6kV, 0.8kV, 1kV, 1.2kV, 1.4kV, 1.6kV, 1.8kV, 2kV, 2.3kV, 2.5kV, 2.8kV or 2.9kV) negative pressure, receive 5 ~ 60min (such as 6min, 10min, 13min, 15min, 20min, 25min, 28min, 30min, 35min, 38min, 40min, 43min, 48min, 50min, 53min, 55min or 58min) obtain internal layer orientation fibers layer, then by bipolar electrode all ground connection, continue reception 5 ~ 60min (such as 6min, 10min, 13min, 15min, 20min, 25min, 28min, 30min, 35min, 38min, 40min, 43min, 48min, 50min, 53min, 55min or 58min) and obtain outer nonwoven No yield point fibrous layer, and cover described internal layer orientation fibers layer.
In the present invention, bipolar electrode is in electrospinning device, and under the driving of motor, bipolar electrode produces rotating Vortex under the drive of belt, by regulating receiving system as mentioned above, namely first by bipolar electrode b in receiving system
1and b
2one of ground connection, another electrode connects 0.5 ~ 3kV negative pressure, receive obtain internal layer orientation fibers layer; Then by bipolar electrode all ground connection, continue reception and obtain outer nonwoven No yield point fibrous layer, and cover described internal layer orientation fibers layer, due to two electrode b
1and b
2between a place be blank spacing, the sleeve pipe of hollow can be obtained after therefore receiving above-mentioned fiber at a place, obtain CO2 laser weld sleeve pipe of the present invention.
Electrostatic spinning apparatus obtains above-mentioned internal layer orientation fibers layer, after the fibrolaminar sleeve pipe of outer nonwoven No yield point, it is taken off from receiving system and just obtains of the present invention there is double-deck CO2 laser weld sleeve pipe.
As the preferred technical solution of the present invention, preparation method of the present invention comprises the following steps:
(1) polymer is dissolved in organic solvent is mixed with the solution that mass body volume concentrations is 1-30%, then stir 12-24h at 50-80 DEG C and obtain polymer solution;
(2) polymer solution is injected electrospinning device and carry out spinning, optimum configurations during spinning is: spinning head voltage is 10 ~ 30kV, and solution flow rate is 1 ~ 5mL/h; The fiber formed penetrates from the spinning head of electrospinning device, receiving range between receiving system and spinning head is 5 ~ 25cm, bipolar electrode is prepared as collecting electrode in receiving system, the axis of bipolar electrode is in parallel arranged on same level line, in described bipolar electrode, the diameter of each electrode is 2 ~ 20mm, bipolar electrode spacing is 1 ~ 10cm, synchronous rotating Vortex, first by one of bipolar electrode ground connection in receiving system, another electrode connects 0.5 ~ 3kV negative pressure, receives 5 ~ 60min and obtains internal layer orientation fibers layer; Then by bipolar electrode all ground connection, continue reception 5 ~ 60min and obtain outer nonwoven No yield point fibrous layer, and cover described internal layer orientation fibers layer, namely obtain described CO2 laser weld sleeve pipe.
The present invention utilizes electrostatic spinning technique, the improvement of receiving system in being prepared by electrostatic spinning, can prepare and of the present invention there is double-deck CO2 laser weld sleeve pipe, its internal layer orientation fibers thickness is 40 ~ 100 μm, outer non-woven fibre thickness is 20 ~ 60 μm, method is simple, easy to operate, is suitable for commercial production.
On the other hand, the invention provides the application of CO2 laser weld sleeve pipe in the material for the preparation of promotion CO2 laser weld as described in relation to the first aspect.The CO2 laser weld sleeve pipe that the present invention prepares has good biocompatibility, has good strength and stability, is material implanted and one of the ideal material that reproduces of human organ.
Relative to prior art, the present invention has following beneficial effect:
CO2 laser weld sleeve pipe of the present invention, neuron can be made to grow along fibre orientation, and then the reparation realized nervous tissue, and outer field nonwoven No yield point polymer fibres layers is played a supporting role, and give CO2 laser weld sleeve pipe of the present invention with good strength and stability, simultaneously because the polymer of ectonexine is biologically absorbable polymer, it has good biocompatibility, chronic inflammatory reaction can not be caused, there is higher safety, when CO2 laser weld sleeve pipe of the present invention is used as peripheral nervous repairing casing, neural axon cell has orientation in its superficial growth, prolong machine direction growth, when after neural ankylose, this sleeve pipe can automatic degraded and absorbed, and the preparation method of CO2 laser weld sleeve pipe of the present invention is simple, suitability for industrialized production can be realized.
Accompanying drawing explanation
The bipolar electrode b of the parallel arranged used in the receiving system of Fig. 1 for electrospinning device of the present invention
1and b
2, a is the interval region between two electrodes.
Detailed description of the invention
Technical scheme of the present invention is further illustrated below by detailed description of the invention.Those skilled in the art should understand, described embodiment is only help to understand the present invention, should not be considered as concrete restriction of the present invention.
Embodiment 1
In this embodiment, prepare CO2 laser weld sleeve pipe by the following method, specifically comprise the following steps:
(1) polycaprolactone being dissolved in volume ratio is be mixed with the solution that mass body volume concentrations is 30% in the chloroform of 1:1 and the mixed liquor of DMF, then stirs 24h at 50 DEG C and obtains polymer solution;
(2) polymer solution is injected electrospinning device by syringe (with No. 5 rustless steel syringe needles, diameter 1-5mm) and carry out spinning, optimum configurations during spinning is: spinning head voltage is 10kV, and solution flow rate is 5mL/h; The fiber formed penetrates from the spinning head of electrospinning device, receiving range between receiving system and spinning head is 5cm, bipolar electrode is prepared as collecting electrode in receiving system, the axis of bipolar electrode is in parallel arranged (as shown in Figure 1) on same level line, in described bipolar electrode, the diameter of each electrode is 20mm, bipolar electrode spacing is 10cm, synchronous rotating Vortex, first by one of bipolar electrode ground connection in receiving system, another electrode connects 0.5kV negative pressure, receives 10min and obtains internal layer orientation fibers layer; Then by bipolar electrode all ground connection, continue to receive 5min and obtain outer nonwoven No yield point fibrous layer, and cover described internal layer orientation fibers layer, it is taken off from receiving system, namely CO2 laser weld sleeve pipe is obtained, the internal layer orientation fibers layer thickness of this sleeve pipe is 40 μm, and outer non-woven fibre thickness is 20 μm, and the diameter of polymer fiber is 100 ~ 300nm.
Embodiment 2
In this embodiment, prepare CO2 laser weld sleeve pipe by the following method, specifically comprise the following steps:
(1) glycolide-lactide copolymer (glycolide content is greater than 80%) is dissolved in the chloroform and N that volume ratio is 2:1, be mixed with the solution that mass body volume concentrations is 10% in the mixed liquor of dinethylformamide, then stir 20h at 60 DEG C and obtain polymer solution;
(2) polymer solution is injected electrospinning device by syringe (with No. 5 rustless steel syringe needles, diameter 1-5mm) and carry out spinning, optimum configurations during spinning is: spinning head voltage is 20kV, and solution flow rate is 3mL/h; The fiber formed penetrates from the spinning head of electrospinning device, receiving range between receiving system and spinning head is 10cm, bipolar electrode is prepared as collecting electrode in receiving system, the axis of bipolar electrode is in parallel arranged (as shown in Figure 1) on same level line, in described bipolar electrode, the diameter of each electrode is 10mm, bipolar electrode spacing is 5cm, synchronous rotating Vortex, first by one of bipolar electrode ground connection in receiving system, another electrode connects 3kV negative pressure, receives 5min and obtains internal layer orientation fibers layer; Then by bipolar electrode all ground connection, continue to receive 5min and obtain outer nonwoven No yield point fibrous layer, and cover described internal layer orientation fibers layer, it is taken off from receiving system, namely CO2 laser weld sleeve pipe is obtained, the internal layer orientation fibers layer thickness of this sleeve pipe is 50 μm, and outer non-woven fibre thickness is 30 μm, and the diameter of polymer fiber is 200 ~ 500nm.
Embodiment 3
In this embodiment, prepare CO2 laser weld sleeve pipe by the following method, specifically comprise the following steps:
(1) caprolactone-glycolide copolymer (caprolactone content is greater than 70%) is dissolved in the chloroform and N that volume ratio is 3:1, be mixed with the solution that mass body volume concentrations is 1% in the mixed liquor of dinethylformamide, then stir 12h at 80 DEG C and obtain polymer solution;
(2) polymer solution is injected electrospinning device by syringe (with No. 5 rustless steel syringe needles, diameter 1-5mm) and carry out spinning, optimum configurations during spinning is: spinning head voltage is 30kV, and solution flow rate is 1mL/h; The fiber formed penetrates from the spinning head of electrospinning device, receiving range between receiving system and spinning head is 25cm, bipolar electrode is prepared as collecting electrode in receiving system, the axis of bipolar electrode is in parallel arranged (as shown in Figure 1) on same level line, in described bipolar electrode, the diameter of each electrode is 2mm, bipolar electrode spacing is 5cm, synchronous rotating Vortex, first by one of bipolar electrode ground connection in receiving system, another electrode connects 1.5kV negative pressure, receives 60min and obtains internal layer orientation fibers layer; Then by bipolar electrode all ground connection, continue to receive 25min and obtain outer nonwoven No yield point fibrous layer, and cover described internal layer orientation fibers layer, it is taken off from receiving system, namely CO2 laser weld sleeve pipe is obtained, the internal layer orientation fibers layer thickness of this sleeve pipe is 100 μm, and outer non-woven fibre thickness is 40 μm, and the diameter of polymer fiber is 400 ~ 800nm.
Embodiment 4
In this embodiment, prepare CO2 laser weld sleeve pipe by the following method, specifically comprise the following steps:
(1) Ju diethyleno dioxide ketone is dissolved in hexafluoroisopropanol and is mixed with the solution that mass body volume concentrations is 15%, then stir 15h at 70 DEG C and obtain polymer solution;
(2) polymer solution is injected electrospinning device by syringe (with No. 5 rustless steel syringe needles, diameter 1-5mm) and carry out spinning, optimum configurations during spinning is: spinning head voltage is 25kV, and solution flow rate is 2mL/h; The fiber formed penetrates from the spinning head of electrospinning device, receiving range between receiving system and spinning head is 15cm, bipolar electrode is prepared as collecting electrode in receiving system, the axis of bipolar electrode is in parallel arranged (as shown in Figure 1) on same level line, in described bipolar electrode, the diameter of each electrode is 10mm, bipolar electrode spacing is 10cm, synchronous rotating Vortex, first by one of bipolar electrode ground connection in receiving system, another electrode connects 1.5kV negative pressure, receives 30min and obtains internal layer orientation fibers layer; Then by bipolar electrode all ground connection, continue to receive 60min and obtain outer nonwoven No yield point fibrous layer, and cover described internal layer orientation fibers layer, it is taken off from receiving system, namely CO2 laser weld sleeve pipe is obtained, the internal layer orientation fibers layer thickness of this sleeve pipe is 90 μm, and outer non-woven fibre thickness is 60 μm, and the diameter of polymer fiber is 500 ~ 1000nm.
Embodiment 5
In this embodiment, prepare CO2 laser weld sleeve pipe by the following method, specifically comprise the following steps:
(1) Ju diethyleno dioxide ketone is dissolved in hexafluoroisopropanol and is mixed with the solution that mass body volume concentrations is 10%, then stir 20h at 50 DEG C and obtain polymer solution;
(2) polymer solution is injected electrospinning device by syringe (with No. 5 rustless steel syringe needles, diameter 1-5mm) and carry out spinning, optimum configurations during spinning is: spinning head voltage is 30kV, and solution flow rate is 5mL/h; The fiber formed penetrates from the spinning head of electrospinning device, receiving range between receiving system and spinning head is 10cm, bipolar electrode is prepared as collecting electrode in receiving system, the axis of bipolar electrode is in parallel arranged (as shown in Figure 1) on same level line, in described bipolar electrode, the diameter of each electrode is 10mm, bipolar electrode spacing is 8cm, synchronous rotating Vortex, first by one of bipolar electrode ground connection in receiving system, another electrode connects 0.5kV negative pressure, receives 60min and obtains internal layer orientation fibers layer; Then by bipolar electrode all ground connection, continue to receive 60min and obtain outer nonwoven No yield point fibrous layer, and cover described internal layer orientation fibers layer, it is taken off from receiving system, namely CO2 laser weld sleeve pipe is obtained, the internal layer orientation fibers layer thickness of this sleeve pipe is 60 μm, and outer non-woven fibre thickness is 30 μm, and the diameter of polymer fiber is 300 ~ 500nm.
Embodiment 6
In this embodiment, prepare CO2 laser weld sleeve pipe by the following method, specifically comprise the following steps:
(1) polylactic acid being dissolved in volume ratio is be mixed with the solution that mass body volume concentrations is 20% in the chloroform of 1:1 and the mixed liquor of DMF, then stirs 24h at 50 DEG C and obtains polymer solution;
(2) polymer solution is injected electrospinning device by syringe (with No. 5 rustless steel syringe needles, diameter 1-5mm) and carry out spinning, optimum configurations during spinning is: spinning head voltage is 10kV, and solution flow rate is 5mL/h; The fiber formed penetrates from the spinning head of electrospinning device, receiving range between receiving system and spinning head is 5cm, bipolar electrode is prepared as collecting electrode in receiving system, the axis of bipolar electrode is in parallel arranged (as shown in Figure 1) on same level line, in described bipolar electrode, the diameter of each electrode is 10mm, bipolar electrode spacing is 8cm, synchronous rotating Vortex, first by one of bipolar electrode ground connection in receiving system, another electrode connects 2kV negative pressure, receives 30min and obtains internal layer orientation fibers layer; Then by bipolar electrode all ground connection, continue to receive 40min and obtain outer nonwoven No yield point fibrous layer, and cover described internal layer orientation fibers layer, it is taken off from receiving system, namely CO2 laser weld sleeve pipe is obtained, the internal layer orientation fibers layer thickness of this sleeve pipe is 50 μm, and outer non-woven fibre thickness is 25 μm, and the diameter of polymer fiber is 200 ~ 500nm.
The CO2 laser weld sleeve pipe prepared by embodiment of the present invention 1-6 is used as peripheral nervous repairing casing, and neural axon cell prolongs machine direction growth in its superficial growth with having orientation, and when after neural ankylose, this sleeve pipe can automatic degraded and absorbed.
Applicant states, the present invention illustrates CO2 laser weld sleeve pipe of the present invention and its preparation method and application by above-described embodiment, but the present invention is not limited to above-described embodiment, does not namely mean that the present invention must rely on above-described embodiment and could implement.Person of ordinary skill in the field should understand, any improvement in the present invention, to equivalence replacement and the interpolation of auxiliary element, the concrete way choice etc. of each raw material of product of the present invention, all drops within protection scope of the present invention and open scope.
Claims (10)
1. a CO2 laser weld sleeve pipe, is characterized in that, described CO2 laser weld sleeve pipe is double layer fibre sleeve pipe, and the internal layer of described sleeve pipe is orientated polymer fibrous layer, and skin is nonwoven No yield point polymer fibres layers, and described polymer is biologically absorbable polymer.
2. CO2 laser weld sleeve pipe according to claim 1, it is characterized in that, described polymer is the combination of any one or at least two kinds in polycaprolactone, polylactic acid, glycolide-lactide copolymer, caprolactone-glycolide copolymer, caprolactone-carbonate copolymer or Ju diethyleno dioxide ketone;
Preferably, the relative molecular weight of described polymer is 60,000-200 ten thousand;
Preferably, described glycolide-lactide copolymer is the glycolide-lactide copolymer that glycolide content is greater than 80%;
Preferably, described caprolactone-glycolide copolymer is caprolactone-glycolide copolymer that caprolactone content is greater than 70%
Preferably, described caprolactone-carbonate copolymer is caprolactone-carbonate copolymer that caprolactone content is greater than 70%.
3. CO2 laser weld sleeve pipe according to claim 1 and 2, is characterized in that, the diameter of described polymer fiber is 100 ~ 5000nm, preferably 100 ~ 1000nm, further preferred 100 ~ 500nm;
Preferably, the thickness of described internal layer is 40 ~ 100 μm, preferably 50 ~ 100 μm;
Preferably, described outer layer thickness is 20 ~ 60 μm;
Preferably, described internal layer thickness is 1.5-3 times of outer layer thickness, is preferably 2 times.
4. the preparation method of the CO2 laser weld sleeve pipe according to any one of claim 1-3, it is characterized in that, described method is: polymer solution is injected electrospinning device and carry out spinning, the fiber formed penetrates from the spinning head of electrospinning device, regulate receiving system to obtain internal layer orientated polymer fibrous layer and outer nonwoven No yield point polymer fibres layers respectively, namely obtain described CO2 laser weld sleeve pipe.
5. preparation method according to claim 4, it is characterized in that, the preparation method of described polymer solution is: be dissolved in by polymer in organic solvent and be mixed with the solution that mass body volume concentrations is 1-30%, then stirs 12-24h at 50-80 DEG C and obtains described polymer solution;
Preferably, described organic solvent is the mixed liquor of hexafluoroisopropanol or chloroform and DMF;
Preferably, in the mixed liquor of described chloroform and DMF, the volume ratio of chloroform and DMF is 1:1-1:3.
6. the preparation method according to claim 4 or 5, is characterized in that, optimum configurations when described electrospinning device carries out spinning is: spinning head voltage is 10 ~ 30kV, and solution flow rate is 1 ~ 5mL/h;
Preferably, the receiving range between described receiving system and spinning head is 5 ~ 25cm.
7. the preparation method according to any one of claim 4-6, it is characterized in that, bipolar electrode is prepared as collecting electrode in described receiving system, the axis of bipolar electrode is in parallel arranged on same level line, in described bipolar electrode, the diameter of each electrode is 2 ~ 20mm, bipolar electrode spacing is 1 ~ 10cm, synchronous rotating Vortex.
8. the preparation method according to any one of claim 4-7, it is characterized in that, the method that described adjustment receiving system obtains internal layer orientated polymer fibrous layer and outer nonwoven No yield point polymer fibres layers is respectively: first by one of bipolar electrode ground connection in receiving system, another electrode connects 0.5 ~ 3kV negative pressure, receives 5 ~ 60min and obtains internal layer orientated polymer fibrous layer; Then by bipolar electrode all ground connection, continue reception 5 ~ 60min and obtain outer nonwoven No yield point polymer fibres layers, and cover described internal layer orientated polymer fibrous layer.
9. the preparation method according to any one of claim 4-8, is characterized in that, said method comprising the steps of:
(1) polymer is dissolved in organic solvent is mixed with the solution that mass body volume concentrations is 1-30%, then stir 12-24h at 50-80 DEG C and obtain polymer solution;
(2) polymer solution is injected electrospinning device and carry out spinning, optimum configurations during spinning is: spinning head voltage is 10 ~ 30kV, and solution flow rate is 1 ~ 5mL/h; The fiber formed penetrates from the spinning head of electrospinning device, receiving range between receiving system and spinning head is 5 ~ 25cm, bipolar electrode is prepared as collecting electrode in receiving system, the axis of bipolar electrode is in parallel arranged on same level line, in described bipolar electrode, the diameter of each electrode is 2 ~ 20mm, bipolar electrode spacing is 1 ~ 10cm, synchronous rotating Vortex, first by one of bipolar electrode ground connection in receiving system, another electrode connects 0.5 ~ 3kV negative pressure, receives 5 ~ 60min and obtains internal layer orientated polymer fibrous layer; Then by bipolar electrode all ground connection, continue reception 5 ~ 60min and obtain outer nonwoven No yield point polymer fibres layers, and cover described internal layer orientated polymer fibrous layer, namely obtain described CO2 laser weld sleeve pipe.
10. the application of the CO2 laser weld sleeve pipe according to any one of claim 1-3 in the material for the preparation of promotion CO2 laser weld.
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