CN111607501A - Vegetable leaf microorganism collection system - Google Patents

Vegetable leaf microorganism collection system Download PDF

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Publication number
CN111607501A
CN111607501A CN202010505938.4A CN202010505938A CN111607501A CN 111607501 A CN111607501 A CN 111607501A CN 202010505938 A CN202010505938 A CN 202010505938A CN 111607501 A CN111607501 A CN 111607501A
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CN
China
Prior art keywords
sampling
microorganism
sampling vessel
outlet
vessel
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Pending
Application number
CN202010505938.4A
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Chinese (zh)
Inventor
潘阳露
赵静
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Chongqing Technology and Business University
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Chongqing Technology and Business University
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Priority to CN202010505938.4A priority Critical patent/CN111607501A/en
Publication of CN111607501A publication Critical patent/CN111607501A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/52Mobile; Means for transporting the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/02Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops

Abstract

The invention relates to the field of microorganism collecting devices, and particularly discloses a vegetable leaf microorganism collecting device which comprises a sampling vessel, wherein the sampling vessel comprises a sampling vessel bottom and a sampling vessel cover, the sampling vessel cover can be covered on the sampling vessel bottom in an adaptive manner, the inner wall of the sampling vessel cover can be attached to the outer wall of the sampling vessel bottom, a first blade inlet and outlet is formed in the edge of the side surface of the sampling vessel bottom, a microorganism solid culture medium is filled in the sampling vessel bottom, the upper surface of the microorganism solid culture medium is flush with the bottom end of the first blade inlet and outlet, a second blade inlet and outlet is formed in the sampling vessel cover, and when the sampling vessel bottom and the sampling vessel cover are completely covered, the first blade inlet and outlet and the second blade inlet and outlet can be completely overlapped or mutually staggered through the relative rotation of the sampling vessel cover and the sampling vessel bottom. According to the invention, the sampling vessel filled with the solid culture medium is arranged, so that microorganisms are directly collected on the vegetable leaves and are directly used for culture after collection, and the types of collected microorganisms are increased.

Description

Vegetable leaf microorganism collection system
Technical Field
The invention relates to the field of microorganism collection devices, and particularly discloses a vegetable leaf microorganism collection device.
Background
In the prior art, the plant is generally taken down for collecting the microorganisms on the plant, and the microorganisms are collected by a series of means such as shearing, scraping and the like in a laboratory, but in the process, a large number of microorganisms are omitted, so that the types of the collected microorganisms are rare, and the obligate parasitic bacteria of a part of vegetable leaves are difficult to collect, so that the invention provides the vegetable leaf microorganism collecting device.
Disclosure of Invention
In view of the technical shortcomings, the invention aims to provide a vegetable leaf microorganism collecting device, which solves the problems pointed out in the background technology.
In order to solve the technical problems, the invention adopts the following technical scheme:
the invention provides a vegetable leaf microorganism collecting device which is characterized by comprising a sampling vessel;
the sampling vessel comprises a sampling vessel bottom and a sampling vessel cover, the sampling vessel cover can be covered on the sampling vessel bottom in an adaptive manner, and the inner wall of the sampling vessel cover can be attached to the outer wall of the sampling vessel bottom;
a first blade inlet and outlet is formed in the edge of the side face of the bottom of the sampling dish, a microorganism solid culture medium is filled in the bottom of the sampling dish, and the upper surface of the microorganism solid culture medium is flush with the bottom end of the first blade inlet and outlet;
the sampling vessel cover is provided with a second blade inlet and a second blade outlet, when the sampling vessel bottom and the sampling vessel cover are completely covered, the sampling vessel cover and the sampling vessel bottom rotate relatively, and the first blade inlet and the second blade outlet can be completely overlapped or staggered.
Preferably, the caliber length of the inlet and outlet of the first blade is less than one fourth of the perimeter of the edge of the bottom side surface of the sampling vessel;
the length of the diameter of the second blade inlet/outlet is equal to the length of the diameter of the first blade inlet/outlet (101).
Preferably, the bottom end of the inlet/outlet of the first blade is positioned at one fourth to one third of the side edge of the bottom of the sampling dish.
Preferably, the invention also comprises a microorganism collection box, wherein the sampling vessel is arranged in the microorganism collection box, the side wall of the microorganism collection box is provided with a sampling port, a sampling vessel feeding port and a sampling vessel collection port, and a sampling vessel support mechanism is arranged in the microorganism collection box;
the sampling vessel supporting mechanism comprises an air cylinder, a sampling platform is fixedly connected with an extending end of the air cylinder, the sampling vessel is arranged on the sampling platform, a cross-shaped clamping groove is formed in the upper surface of a sampling vessel cover, the sampling platform is close to the sampling port and is positioned on the same horizontal plane with the sampling port when the air cylinder is in an extending state, and the sampling platform is close to the sampling vessel collecting port and is positioned on the same horizontal plane with the sampling vessel collecting port when the air cylinder is in a shortening state;
the microorganism collection box is characterized in that a sliding groove is transversely formed in the upper wall of the microorganism collection box, a rotating shaft is connected in the sliding groove in a sliding mode, a manual knob is fixedly connected to the upper end of the rotating shaft, the width of the bottom of the manual knob is larger than that of the sliding groove, a cross limiting piece is fixedly connected to the extending end of the rotating shaft, and the cross limiting piece can be clamped into the cross clamping groove in a matching mode.
Preferably, a conveying mechanism is further arranged in the microorganism collection box, and the conveying mechanism and the sampling vessel putting port are positioned on the same horizontal plane;
the conveying mechanism comprises a driving roller, a driven roller and a conveying belt, and the conveying belt is sleeved on the outer sides of the driving roller and the driven roller in a matched manner;
one end of the driving roller is fixedly connected with a connecting rod, the extending end of the connecting rod penetrates through the front wall of the microorganism collection box and is fixedly connected with a rotating motor, the rotating motor is fixedly connected to the outer wall of the microorganism collection box, and the other end of the driving roller penetrates through the rear wall of the microorganism collection box and is rotatably connected with the rear wall of the microorganism collection box;
the inside dead lever that is equipped with of driven cylinder, the dead lever both ends respectively fixed connection be in on the relative inner wall that sets up of microorganism collection box, driven cylinder cover is established outside the dead lever.
Preferably, the top wall of the microorganism collecting box is provided with a sliding groove in the transverse direction, the bottom width of the manual knob is larger than the width of the sliding groove, and the rotating shaft can move longitudinally and transversely in the sliding groove.
Preferably, a plurality of bulges are arranged on the walls of the driving roller and the driven roller.
Preferably, the microorganism collection box is a transparent box body.
The invention has the beneficial effects that: according to the microorganism collecting device, the sampling vessel filled with the microorganism solid culture medium is arranged, microorganisms are directly collected on the vegetable leaves and directly used for culture after collection, so that a great amount of omission of the microorganisms caused by complicated steps in the processes of collecting the microorganisms and transferring the microorganisms is reduced, and the types of the collected microorganisms are complete; still realize semi-automatization's collection through setting up sampling ware supporting mechanism and transport mechanism in the microorganism collection box for gather labour saving and time saving more.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural diagram of a sampling vessel of a vegetable leaf microorganism collection device according to an embodiment of the present invention;
FIG. 2 is a schematic view of an external structure of the sampling vessel cover covered on the bottom of the sampling vessel in FIG. 1;
FIG. 3 is a schematic structural view of a microorganism collection box of a microorganism collection device for vegetable leaves according to an embodiment of the present invention;
FIG. 4 is a schematic structural view of the cross-shaped spacing piece in FIG. 3;
FIG. 5 is a schematic structural view of the transfer mechanism of FIG. 3;
description of reference numerals: 1-sampling dish bottom, 2-sampling dish cover, 3-microorganism collection box, 4-cylinder, 5-conveying mechanism, 101-first blade inlet and outlet, 102-microorganism solid culture medium, 201-cross clamping groove, 301-sampling port, 302-sampling dish feeding port, 303-sampling dish collection port, 401-sampling table, 402-manual knob, 403-rotating shaft, 4031-cross limiting sheet, 501-rotating motor, 502-connecting rod, 503-driving roller, 504-fixing rod, 505-driven roller and 506-conveying belt.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of describing technical solutions of the present invention and simplifying the description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
As shown in fig. 1-5, a vegetable leaf microorganism collecting device comprises a sampling vessel;
the sampling dish comprises a sampling dish bottom 1 and a sampling dish cover 2, the sampling dish cover 2 can be covered on the sampling dish bottom 1 in a matching manner, and the inner wall of the sampling dish cover 2 can be attached to the outer wall of the sampling dish bottom 1;
a first blade inlet and outlet 101 is formed in the edge of the side face of the sampling dish bottom 1, a microorganism solid culture medium 102 is arranged in the sampling dish bottom 1, and the upper surface of the microorganism solid culture medium 102 is flush with the bottom end of the first blade inlet and outlet 101;
the sampling dish cover 2 is provided with a second blade inlet and outlet, when the sampling dish bottom 1 and the sampling dish cover 2 are completely covered, the first blade inlet and outlet 101 and the second blade inlet and outlet can be completely overlapped or staggered through the relative rotation of the sampling dish cover 2 and the sampling dish bottom 1.
During the use, to rotating sampling dish lid 2 and sampling dish bottom 1 relatively behind the vegetables blade next door for the coincidence is imported and exported to first blade 101 and second blade, then stretch into the sampling dish with the vegetables blade, paste gently on the microorganism solid culture medium 102 that has adorned at sampling dish bottom 1 in advance, take out the blade after keeping 5-10 seconds, then resume rapidly that first blade is imported and exported 101 and second blade and import and export and stagger each other, then put into the incubator in the laboratory and invert the cultivation.
In this embodiment, the aperture length of the first vane inlet/outlet 101 is less than one fourth of the perimeter of the edge of the side surface of the sampling vessel bottom 1;
the length of the opening of the second blade inlet and outlet is equal to that of the first blade inlet and outlet 101, so that the first blade inlet and outlet 101 and the second blade inlet and outlet can be completely staggered while other mixed bacteria pollution caused by overlong opening is reduced.
The bottom end of the first blade inlet and outlet 101 is located at one fourth to one third of the side edge of the sampling dish bottom 1, and a volume range is reserved according to the amount of the microorganism solid culture medium 102, so that the sampling dish bottom 1 can contain the amount of the culture medium required by microorganism growth, and meanwhile, the first blade inlet and outlet 101 can be ensured to pass through the vegetable blades.
In this embodiment, the microorganism collecting device provided by the invention further comprises a microorganism collecting box 3, the sampling vessel is installed in the microorganism collecting box 3, a sampling port 301, a sampling vessel putting port 302 and a sampling vessel collecting port 303 are formed in the side wall of the microorganism collecting box 3, and a sampling vessel supporting mechanism is further arranged in the microorganism collecting box 3;
the sampling vessel supporting mechanism comprises an air cylinder 4, a sampling table 401 is fixedly connected to an extending end of the air cylinder 4, the sampling vessel is arranged on the sampling table 401, a cross-shaped clamping groove 201 is formed in the upper surface of the sampling vessel cover 2, the sampling table 401 is close to the sampling port 301 and is positioned on the same horizontal plane with the sampling port 301 when the air cylinder 4 is in an extending state, and the sampling table 401 is close to the sampling vessel collecting port 303 and is positioned on the same horizontal plane with the sampling vessel collecting port 303 when the air cylinder 4 is in a shortening state;
the top wall of the microorganism collection box 3 is provided with a manual knob 402, the bottom of the manual knob 402 is fixedly connected with a rotating shaft 403, the extending end of the rotating shaft 403 is further fixedly connected with a cross limiting sheet 4031, the cross limiting sheet 4031 can be clamped into the cross clamping groove 201 in an adaptive manner, the sampling table 401 extends to the sampling port 301 through the extension of the air cylinder 4, then vegetable leaves are inserted through the sampling port and enter the inside of the sampling dish through the superposed first leaf inlet and outlet 101 and second leaf inlet and outlet, so that the vegetable leaves can be taken out after being pasted on the microorganism solid culture medium 102 for 5-10 seconds, then the manual knob 402 is manually rotated, the cross limiting sheet 4031 acts on the cross clamping groove 201, the sampling dish cover 2 is rotated, the first leaf inlet and outlet 101 and the second leaf inlet and outlet are staggered mutually, and then the air cylinder is shortened, the sample vessel is removed from the sample vessel collection port 303.
In this embodiment, a conveying mechanism 5 is further disposed in the microorganism collection box 3, and the conveying mechanism 5 and the sample vessel input port 302 are located on the same horizontal plane;
the conveying mechanism 5 comprises a driving roller 503, a driven roller 505 and a conveying belt 506, and the conveying belt 506 is fittingly sleeved on the outer sides of the driving roller 503 and the driven roller 505;
one end of the driving roller 503 is fixedly connected with a connecting rod 502, the extending end of the connecting rod 502 penetrates through the front wall of the microorganism collection box 3 and is fixedly connected with a rotating motor 501, the rotating motor 501 is fixedly connected to the outer wall of the microorganism collection box 3, and the other end of the driving roller 503 penetrates through the rear wall of the microorganism collection box 3 and is rotatably connected with the rear wall of the microorganism collection box 3;
a fixed rod 504 is arranged in the driven roller 505, two ends of the fixed rod 504 are respectively and fixedly connected to the inner walls of the microorganism collecting box 3 which are oppositely arranged, and the driven roller 505 is sleeved outside the fixed rod 504;
the sampling vessel is put in from a sampling vessel putting port 302 and placed on a conveying belt 506, a rotating motor 501 is started, a connecting rod 502 is driven to rotate through the rotating motor 501, a driving roller 503 is driven to rotate, the driving roller 503 and the conveying belt 506 interact, a driven roller 505 is driven to rotate, the sampling vessel is conveyed to a sampling platform 401, a first blade inlet and outlet 101 and a second blade inlet and outlet are overlapped through a manual knob 402 and then sampled, after sampling is finished, the first blade inlet and outlet 101 and the second blade inlet and outlet are staggered through the manual knob 402, an air cylinder is shortened, the sampling vessel is taken out from a sampling vessel collecting port 303, the sampling vessel is sealed through a sealing film according to the conventional operation of the microbial field, pollution is prevented, and a target strain is separated after being transported to an experiment room for inverted culture.
The top wall of the microorganism collection box 3 transversely starts to be provided with a sliding groove, the width of the bottom of the manual knob 402 is larger than that of the sliding groove, the rotating shaft 403 can longitudinally and transversely move in the sliding groove, and the central part of the sampling table 401 can be better placed in a sampling vessel by assisting the sampling vessel in longitudinally and transversely moving the rotating shaft 403, so that the sampling is more convenient.
The walls of the driving roller 503 and the driven roller 505 are both provided with a plurality of protrusions, so that the friction force between the driving roller 503 and the driven roller 505 and the conveyor belt 506 is increased, and the slippage is prevented.
The microorganism collection box 3 is a transparent box body, so that the sampling process is transparent and convenient to observe.
It should be noted that the microorganism solid culture medium 102 in the present invention can be a plurality of culture mediums, and in the actual sampling process, a plurality of sampling dishes need to be prepared, and a culture medium corresponding to the target microorganism needs to be prepared in advance.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.

Claims (8)

1. A vegetable leaf microorganism collection device is characterized by comprising a sampling vessel;
the sampling vessel comprises a sampling vessel bottom (1) and a sampling vessel cover (2), the sampling vessel cover (2) can be covered on the sampling vessel bottom (1) in an adaptive manner, and the inner wall of the sampling vessel cover (2) can be attached to the outer wall of the sampling vessel bottom (1);
a first blade inlet and outlet (101) is formed in the edge of the side face of the sampling dish bottom (1), a microorganism solid culture medium (102) is arranged in the sampling dish bottom (1), and the upper surface of the microorganism solid culture medium (102) is flush with the bottom end of the first blade inlet and outlet (101);
the sampling dish cover (2) is provided with a second blade inlet and outlet, when the sampling dish bottom (1) and the sampling dish cover (2) are completely covered, the first blade inlet and outlet (101) and the second blade inlet and outlet can be completely overlapped or staggered through the relative rotation of the sampling dish cover (2) and the sampling dish bottom (1).
2. The microorganism collection device according to claim 1, wherein the aperture length of the first vane access (101) is less than one quarter of the perimeter of the edge of the side surface of the sampling dish bottom (1);
the length of the diameter of the second blade inlet/outlet is equal to the length of the diameter of the first blade inlet/outlet (101).
3. The microorganism collection device according to claim 2, wherein the bottom end of the first vane access (101) is located at one quarter to one third of the side edge of the sampling vessel bottom (1).
4. A microorganism collecting device according to any one of claims 1 to 3, further comprising a microorganism collecting box (3), wherein the sampling vessel is installed in the microorganism collecting box (3), a sampling port (301), a sampling vessel placing port (302) and a sampling vessel collecting port (303) are opened on a side wall of the microorganism collecting box (3), and a sampling vessel supporting mechanism is further provided inside the microorganism collecting box (3);
the sampling vessel supporting mechanism comprises an air cylinder (4), a sampling table (401) is fixedly connected to an extending end of the air cylinder (4), the sampling vessel is arranged on the sampling table (401), a cross-shaped clamping groove (201) is formed in the upper surface of the sampling vessel cover (2), when the air cylinder (4) is in an extension state, the sampling table (401) is close to the sampling port (301) and is positioned on the same horizontal plane with the sampling port (301), and when the air cylinder (4) is in a shortening state, the sampling table (401) is close to the sampling vessel collecting port (303) and is positioned on the same horizontal plane with the sampling vessel collecting port (303);
the microorganism collection box is characterized in that a manual knob (402) is arranged on the top wall of the microorganism collection box (3), a rotating shaft (403) is fixedly connected to the bottom of the manual knob (402), a cross-shaped limiting piece (4031) is fixedly connected to the extending end of the rotating shaft (403), and the cross-shaped limiting piece (4031) can be clamped into the cross-shaped clamping groove (201) in an adaptive mode.
5. The microorganism collection device according to claim 4, wherein a conveying mechanism (5) is further arranged in the microorganism collection box (3), and the conveying mechanism (5) is positioned on the same horizontal plane with the sample vessel feeding port (302);
the conveying mechanism (5) comprises a driving roller (503), a driven roller (505) and a conveying belt (506), and the conveying belt (506) is fittingly sleeved on the outer sides of the driving roller (503) and the driven roller (505);
one end of the driving roller (503) is fixedly connected with a connecting rod (502), the extending end of the connecting rod (502) penetrates through the front wall of the microorganism collection box (3) and is fixedly connected with a rotating motor (501), the rotating motor (501) is fixedly connected to the outer wall of the microorganism collection box (3), and the other end of the driving roller (503) penetrates through the rear wall of the microorganism collection box (3) and is rotatably connected with the rear wall of the microorganism collection box (3);
a fixing rod (504) is arranged in the driven roller (505), two ends of the fixing rod (504) are respectively and fixedly connected to the inner wall of the microorganism collection box (3) which is arranged oppositely, and the driven roller (505) is sleeved outside the fixing rod (504).
6. The microorganism collection apparatus as claimed in claim 5, wherein the microorganism collection box (3) has a top wall with a transverse slot, the bottom width of the manual knob (402) is greater than the width of the slot, and the rotation shaft (403) can move longitudinally and transversely in the slot.
7. The microorganism collecting device as claimed in claim 6, wherein the driving roller (503) and the driven roller (505) are provided with protrusions on the wall thereof.
8. The microorganism collection apparatus according to claim 7, wherein the microorganism collection tank (3) is a transparent tank.
CN202010505938.4A 2020-06-05 2020-06-05 Vegetable leaf microorganism collection system Pending CN111607501A (en)

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