CN111579306B - Sampling device capable of reducing cell pollution probability in submission process - Google Patents
Sampling device capable of reducing cell pollution probability in submission process Download PDFInfo
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- CN111579306B CN111579306B CN202010568724.1A CN202010568724A CN111579306B CN 111579306 B CN111579306 B CN 111579306B CN 202010568724 A CN202010568724 A CN 202010568724A CN 111579306 B CN111579306 B CN 111579306B
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
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Abstract
The invention relates to the technical field of cell sampling, in particular to a sampling device capable of reducing the probability of cell pollution in the process of censorship, which comprises a censorship box, wherein the censorship box consists of a bottom plate, a base plate and a fixing frame, the base plate is fixedly bonded at the upper end of the bottom plate in parallel, and a mounting inner hole which is sunken downwards is arranged in the middle of the upper end surface of the bottom plate, and the sampling device has the advantages that: the negative pressure tube is directly arranged in the submission box, so that the conversion of a sample between the sampling device and the submission device is reduced, the contact with air in the sampling process is avoided, and the pollution probability in the sampling process is reduced; through setting up rotatable pedestal mounting sampling pipe to all set up the rubber buffer with the upper and lower both ends of negative pressure sampling pipe, thereby realize under the prerequisite of not opening the negative pressure pipe, realize the purpose of sample, utilize the cooperation of outer runner and transfer runner, realize the utilization of many sampling points, avoid causing the mutual influence between the sample repeatedly, avoid repetitive pollution simultaneously, improved the accuracy of cell inspection greatly.
Description
Technical Field
The invention relates to the technical field of cell sampling, in particular to a sampling device capable of reducing the probability of cell pollution in the process of submission.
Background
In order to improve the research on the cell layer, the cells of different animals and plants are generally required to be sampled and analyzed, a common sampling device is provided with a sampling needle and the like, and then the sampled samples are placed in a censorship box, so that the cells are inspected and analyzed.
In order to reduce the probability of the contamination of the cells in the process of inspection, in addition to the rigid requirement of cleaning the inspection and sampling tools, sampling tools and the like, the other reason for the contamination of the cells is that impurities in the air are in contact with the cells, so the contact with the air is required to be reduced as much as possible during the sampling and inspection.
Therefore, the sampling device capable of reducing the probability of cell pollution in the process of submission is provided, so as to solve the problem of reducing the contact with air in the process of submission, sampling and sampling.
Disclosure of Invention
The present invention is directed to a sampling device capable of reducing the probability of cell contamination during a censorship process, so as to solve the above-mentioned problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme:
a sampling device capable of reducing the probability of cell pollution in the process of censorship comprises a censorship box, wherein the censorship box consists of a bottom plate, a base plate and a fixed frame, the upper end of the bottom plate is fixedly bonded with the base plate in parallel, a downward sunken installation inner hole is formed in the middle of the upper end face of the bottom plate, the outer edge of the upper end face of the bottom plate is provided with sampling ponds distributed in a circumferential array manner, an inner cavity of the installation inner hole is rotatably provided with a rotating plate, the upper end of the rotating plate is provided with four limiting grooves distributed in a circumferential array manner, a lifting plate is arranged at the upper end of the rotating plate, a limiting inserting column is vertically and downwardly arranged at the position, corresponding to the limiting grooves, of the lower end of the lifting plate, a spring is arranged in a gap between the lower end of the lifting plate and the upper end of the rotating plate, a connecting disc is arranged in the middle of the upper, a sampling needle is vertically inserted into the upper end surface of the connecting disc, and a negative pressure sampling tube is installed at the upper end of the connecting disc;
the fixed sealed up upper end face that bonds at the base plate of lower extreme of fixed frame is provided with the outer runner that distributes with the circumference array of sample cell one-to-one between the lower extreme of fixed frame and the upper end face of base plate, and the inner chamber is vertical to be provided with the inner circle downwards in the middle of the upper end of fixed frame, the inner wall of inner circle is provided with the internal thread, the upper end of negative pressure sampling pipe is provided with the extrusion apron, and the upper end opening of negative pressure sampling pipe is sealed to be provided with the rubber sealing plug, and the lower extreme opening sealing installation of negative pressure sampling pipe has lower rubber sealing plug, and the inner chamber of.
Preferably, a through hole is vertically formed in the middle of the lower end face of the installation inner hole in a downward penetrating mode, a connecting column is arranged in the middle of the lower end face of the rotating plate, and the connecting column is rotatably inserted into an inner cavity of the through hole in an inserting mode.
Preferably, the lower end of the connecting column extends to the lower end face of the bottom plate, a threaded inner hole is formed in the middle of the lower end face of the connecting column, and a rotary table is rotatably connected to the inner cavity thread of the threaded inner hole.
Preferably, the inner cavity in the middle of the lower end of the fixing frame is vertically provided with a circular retainer with the inner diameter being the same as the outer diameter of the negative pressure sampling tube, and the inner wall of the retainer is tightly attached to the outer wall of the negative pressure sampling tube.
Preferably, a pair of rotation handles of bilateral symmetry is provided with to the up end of extrusion apron, and the circular arc outer wall of extrusion apron is provided with the external screw thread, and the lower extreme inner wall of extrusion apron supports in the upper end of negative pressure sampling pipe, has seted up the jack in the middle of the up end of extrusion apron, the external screw thread rotates with the internal thread screw thread to be connected, the inner chamber of jack is pegged graft and is had last rubber sealing plug.
Preferably, the lower extreme of negative pressure sampling pipe is provided with the connection hole, the inner wall of connecting the hole is provided with a pair of constant head tank of bilateral symmetry, the upper end of connection pad is provided with the locating piece of pegging graft with the constant head tank cooperation, the locating piece is fixed to be pegged graft in the constant head tank.
Preferably, the connecting disc is installed in the inner cavity of the connecting inner hole, a sampling needle at the upper end of the connecting disc vertically penetrates through the lower rubber sealing plug, and the upper end of the sampling needle extends into the sampling inner cavity.
Preferably, the spring is vertically installed in a gap between the lifting plate and the rotating plate, the upper end of the spring abuts against the lower end face of the lifting plate, and the lower end of the spring abuts against the upper end face of the rotating plate.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, the negative pressure tube is directly arranged in the submission box, so that the conversion of a sample between the sampling device and the submission device is reduced, the contact with air in the sampling process is avoided, and the pollution probability in the sampling process is reduced;
2. according to the invention, the sampling tube is installed by arranging the rotatable base, and the rubber plugs are arranged at the upper end and the lower end of the negative pressure sampling tube, so that the purpose of sampling is realized on the premise of not opening the negative pressure tube, the utilization of multiple sampling points is realized by utilizing the matching of the outer flow passage and the transfer flow passage, the mutual influence between repeated sampling is avoided, the repeated pollution is avoided, and the accuracy of cell inspection is greatly improved.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic perspective view of the connection between the lifter plate and the rotator plate according to the present invention;
FIG. 3 is a schematic perspective view of a negative pressure sampling tube according to the present invention;
FIG. 4 is a schematic perspective view of an extruded cover plate according to the present invention;
FIG. 5 is a perspective view of the rotating plate of the present invention;
fig. 6 is a schematic perspective view of the lifter plate of the present invention.
In the figure: 1. a delivery and inspection box; 2. a base plate; 3. a substrate; 4. a fixing frame; 5. installing an inner hole; 6. a rotating plate; 7. connecting columns; 8. a turntable; 9. a threaded inner bore; 10. a spring; 11. a through hole; 12. a sampling pool; 13. an outer flow passage; 14. a lifting plate; 15. a sampling needle; 16. a transfer runner; 17. a limiting groove; 18. a negative pressure sampling tube; 19. a rubber sealing plug is arranged; 20. a rubber sealing plug is arranged; 21. sampling an inner cavity; 22. extruding the cover plate; 23. a jack; 24. a handle is rotated; 25. an inner ring; 26. an internal thread; 27. a holder; 28. an external thread; 29. positioning a groove; 30. connecting the inner holes; 31. a connecting disc; 32. positioning blocks; 33. and (5) limiting the inserted column.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1 to 6, the present invention provides a technical solution:
a sampling device capable of reducing the probability of cell contamination in the process of censorship comprises a censorship box 1, wherein the censorship box 1 is composed of a bottom plate 2, a base plate 3 and a fixing frame 4, and the base plate 3 is fixedly bonded at the upper end of the bottom plate 2 in parallel.
Be provided with undercut's installation hole 5 in the middle of the up end of bottom plate 2, the inner chamber of installation hole 5 rotates installs rotor plate 6, and vertical downwardly running has seted up through-hole 11 in the middle of the lower terminal surface of installation hole 5, is provided with spliced pole 7 in the middle of the lower terminal surface of rotor plate 6, and spliced pole 7 rotates the inner chamber of pegging graft at through-hole 11, utilizes through-hole 11 and installation hole 5 to realize the rotation grafting installation of rotor plate 6.
The lower extreme of spliced pole 7 extends to the lower terminal surface of bottom plate 2, has seted up screw thread hole 9 in the middle of the lower terminal surface of spliced pole 7, and the inner chamber screw thread of screw thread hole 9 rotates and is connected with carousel 8, utilizes screw thread hole 9 to realize the fixed mounting of carousel 8 to utilize carousel 8 to realize the rotation regulation of rotor plate 6.
The upper end of the rotating plate 6 is provided with four limiting grooves 17 distributed in a circumferential array manner, the upper end of the rotating plate 6 is provided with a lifting plate 14, the lower end of the lifting plate 14 is vertically downwards provided with a limiting insertion column 33 at a position corresponding to the limiting grooves 17, the limiting insertion column 33 is vertically slidably inserted into an inner cavity of the limiting grooves 17, and the limiting insertion column 33 is matched with the limiting grooves 17 for limiting insertion so as to fix the rotating angle of the lifting plate 14, ensure that the lifting plate 14 and the rotating plate 6 rotate together and prevent the lifting plate 14 from rotating.
Be provided with connection pad 31 in the middle of the up end of lifter plate 14, negative pressure sampling pipe 18 is installed to the upper end of connection pad 31, the lower extreme of negative pressure sampling pipe 18 is provided with connects hole 30, the inner wall of connecting hole 30 is provided with a pair of constant head tank 29 of bilateral symmetry, the upper end of connection pad 31 is provided with the locating piece 32 of pegging graft with constant head tank 29 cooperation, locating piece 32 is fixed to be pegged graft in constant head tank 29, utilize the cooperation of constant head tank 29 and locating piece 32, thereby realize the injectment of connection angle between lifter plate 14 and the negative pressure sampling pipe 18, make negative pressure sampling pipe 18 rotate along with the rotation of.
The vertical grafting of the up end of connection pad 31 has sampling needle 15, connection pad 31 is installed in the inner chamber of connecting hole 30, rubber sealing plug 20 under the vertical running through of sampling needle 15 of connection pad 31 upper end, the upper end of sampling needle 15 extends to in the sampling inner chamber 21, utilize sampling needle 15 to realize under the prerequisite of not opening negative pressure sampling pipe 18, realize the purpose of sample censorship, reduced greatly and the air between the contact, improved the accuracy of detection.
The middle inner cavity of the lower end of the fixing frame 4 is vertically provided with a circular retainer 27 with the inner diameter being the same as the outer diameter of the negative pressure sampling pipe 18, the inner wall of the retainer 27 is tightly attached to the outer wall of the negative pressure sampling pipe 18, and the retainer 27 is utilized to realize the vertical installation of the negative pressure sampling pipe 18.
The fixed sealed up end at base plate 3 that bonds of lower extreme of fixed frame 4, the vertical inner circle 25 that is provided with downwards of inner chamber in the middle of the upper end of fixed frame 4, the inner wall of inner circle 25 is provided with internal thread 26, the upper end of negative pressure sampling pipe 18 is provided with extrusion apron 22, the up end of extrusion apron 22 is provided with a pair of turning handle 24 of bilateral symmetry, the circular arc outer wall of extrusion apron 22 is provided with external screw thread 28, the lower extreme inner wall of extrusion apron 22 supports the upper end at negative pressure sampling pipe 18, utilize the rotation of external screw thread 28 and internal thread 26 on the extrusion apron 22, the realization is to the extrusion downwards of negative pressure sampling pipe.
A spring 10 is arranged in a gap between the lower end of the lifting plate 14 and the upper end of the rotating plate 6, the spring 10 is vertically installed in the gap between the lifting plate 14 and the rotating plate 6, the upper end of the spring 10 abuts against the lower end face of the lifting plate 14, the lower end of the spring 10 abuts against the upper end face of the rotating plate 6, and in the process that the negative pressure sampling pipe 18 extrudes downwards, the spring 10 is compressed, so that the lifting plate 14 moves downwards, and the transit flow channel 16 and the outer flow channel 13 are located at the same height.
The inner chamber of lifter plate 14 transversely is provided with the transfer runner 16, the up end outer fringe of bottom plate 2 is provided with the sample cell 12 that the circumference array distributes, be provided with the outer runner 13 that distributes with the circumference array of sample cell 12 one-to-one between the lower extreme of fixed frame 4 and the up end of base plate 3, rotation through rotor plate 6, realize the intercommunication and the dislocation of transfer runner 16 and outer runner 13, realize the convenient reposition of redundant personnel sample of sample, thereby avoid taking a sample in same position, cause repetitive pollution and mutual interference.
The upper end opening of negative pressure sampling pipe 18 is sealed to be provided with rubber sealing plug 19, rubber sealing plug 20 down is installed to the lower extreme opening sealing of negative pressure sampling pipe 18, the inner chamber of negative pressure sampling pipe 18 is provided with sampling inner chamber 21, jack 23 has been seted up in the middle of the up end of extrusion apron 22, external screw thread 28 is connected with internal thread 26 screw thread rotation, the inner chamber of jack 23 is pegged graft and is had rubber sealing plug 19, through grafting sampling needle tubing on last rubber sealing plug 19, realize the sealed purpose of direct sampling, be convenient for peg graft with sampling needle 15 through lower rubber sealing plug 20.
The working principle is as follows: the rotation of utilizing at first through-hole 11 and installation hole 5 to realize rotor plate 6 is pegged graft the installation, utilize screw hole 9 to realize the fixed mounting of carousel 8, thereby utilize carousel 8 to realize the rotation regulation of rotor plate 6, utilize spacing the spacing cooperation of inserting post 33 and spacing groove 17 to peg graft, thereby the turned angle of fixed lifter plate 14, ensure that lifter plate 14 rotates with rotor plate 6 together, prevent lifter plate 14 rotation, utilize the cooperation of constant head tank 29 and locating piece 32, thereby realize the injectment of being connected the angle between lifter plate 14 and the negative pressure sampling pipe 18, make negative pressure sampling pipe 18 rotate along with the rotation of lifter plate 14.
Utilize holder 27 to realize the vertical installation of negative pressure sampling pipe 18, utilize the rotation of external screw thread 28 and internal thread 26 on the extrusion apron 22, realize the extrusion downwards to negative pressure sampling pipe 18, at the in-process of negative pressure sampling pipe 18 extrusion downwards, compression spring 10, make lifter plate 14 downstream, make transit runner 16 and outer runner 13 be located same height, the decline in-process, be convenient for peg graft with sampling needle 15 through lower rubber sealing plug 20, utilize sampling needle 15 to realize under the prerequisite of not opening negative pressure sampling pipe 18, realize the mesh of sample censorship, the contact between with the air has been reduced greatly, the accuracy of detection has been improved.
Through the rotation of rotating plate 6, realize the intercommunication and the dislocation of transit runner 16 and a plurality of outer runners 13 of circumference array distribution, realize the convenient reposition of redundant personnel sample of sample to avoid taking a sample in same position, cause repeated pollution and mutual interference, thereby reduce the contaminated probability of cell.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. A sampling device capable of reducing the probability of cell contamination in a submission process comprises a submission box (1), and is characterized in that: the inspection box (1) is composed of a bottom plate (2), a base plate (3) and a fixing frame (4), the base plate (3) is fixedly bonded to the upper end of the bottom plate (2) in parallel, a downward sunken installation inner hole (5) is formed in the middle of the upper end face of the bottom plate (2), sampling pools (12) distributed in a circumferential array mode are arranged on the outer edge of the upper end face of the bottom plate (2), a rotating plate (6) is rotatably installed in an inner cavity of the installation inner hole (5), four limiting grooves (17) distributed in the circumferential array mode are formed in the upper end of the rotating plate (6), a lifting plate (14) is arranged at the upper end of the rotating plate (6), limiting insertion columns (33) are vertically and downwardly arranged at positions, corresponding to the limiting grooves (17), of the lower end of the lifting plate (14), springs (10) are arranged in gaps between the lower end of the lifting plate (14) and the upper end of the rotating plate (6), and connecting discs (, a transfer flow channel (16) is transversely arranged in the inner cavity of the lifting plate (14), the limiting insertion column (33) is vertically inserted into the inner cavity of the limiting groove (17) in a sliding manner, a sampling needle (15) is vertically inserted into the upper end face of the connecting disc (31), and a negative pressure sampling tube (18) is mounted at the upper end of the connecting disc (31);
the lower end of the fixing frame (4) is fixedly, hermetically and fixedly bonded on the upper end face of the base plate (3), outer runners (13) which are distributed in a circumferential array manner and correspond to the sampling pools (12) one by one are arranged between the lower end of the fixing frame (4) and the upper end face of the base plate (3), an inner ring (25) is vertically and downwardly arranged in the middle inner cavity of the upper end of the fixing frame (4), internal threads (26) are arranged on the inner wall of the inner ring (25), an extrusion cover plate (22) is arranged at the upper end of the negative pressure sampling tube (18), an upper rubber sealing plug (19) is hermetically arranged at the upper end opening of the negative pressure sampling tube (18), a lower rubber sealing plug (20) is hermetically arranged at the lower end opening of the negative pressure sampling tube (18), and;
a through hole (11) vertically penetrates downwards from the middle of the lower end face of the mounting inner hole (5), a connecting column (7) is arranged in the middle of the lower end face of the rotating plate (6), and the connecting column (7) is rotatably inserted into an inner cavity of the through hole (11); the rotating plate (6) is rotatably inserted and installed by utilizing the through hole (11) and the installation inner hole (5);
the lower end of the connecting column (7) extends to the lower end face of the bottom plate (2), a threaded inner hole (9) is formed in the middle of the lower end face of the connecting column (7), and a rotary table (8) is rotatably connected to the inner cavity of the threaded inner hole (9) in a threaded mode; the fixed installation of the rotary table (8) is realized by utilizing the threaded inner hole (9), so that the rotary adjustment of the rotary plate (6) is realized by utilizing the rotary table (8).
2. The sampling device of claim 1, wherein the sampling device is capable of reducing the probability of contamination of cells during the inspection process, and comprises: the inner cavity in the middle of the lower end of the fixing frame (4) is vertically provided with a circular retainer (27) with the inner diameter being the same as the outer diameter of the negative pressure sampling pipe (18), and the inner wall of the retainer (27) is tightly attached to the outer wall of the negative pressure sampling pipe (18).
3. The sampling device of claim 1, wherein the sampling device is capable of reducing the probability of contamination of cells during the inspection process, and comprises: the utility model discloses a vacuum sampling device, including extrusion apron (22), jack (23) have been seted up to the circular arc outer wall of extrusion apron (22), the upper end of extrusion apron (22) is provided with a pair of turning handle (24) of bilateral symmetry, and the circular arc outer wall of extrusion apron (22) is provided with external screw thread (28), and the lower extreme inner wall of extrusion apron (22) supports the upper end at negative pressure sampling pipe (18), and external screw thread (28) and internal thread (26) screw thread are rotated and are connected, the inner chamber of jack (23) is pegged graft and is had rubber sealing plug (19.
4. The sampling device of claim 1, wherein the sampling device is capable of reducing the probability of contamination of cells during the inspection process, and comprises: the lower extreme of negative pressure sampling pipe (18) is provided with connects hole (30), the inner wall of connecting hole (30) is provided with a pair of constant head tank (29) of bilateral symmetry, the upper end of connection pad (31) is provided with locating piece (32) of pegging graft with constant head tank (29) cooperation, locating piece (32) are fixed to be pegged graft in constant head tank (29).
5. The sampling device of claim 1, wherein the sampling device is capable of reducing the probability of contamination of cells during the inspection process, and comprises: the connecting disc (31) is installed in an inner cavity of the connecting inner hole (30), a sampling needle (15) at the upper end of the connecting disc (31) vertically penetrates through the lower rubber sealing plug (20), and the upper end of the sampling needle (15) extends into the sampling inner cavity (21).
6. The sampling device of claim 1, wherein the sampling device is capable of reducing the probability of contamination of cells during the inspection process, and comprises: the spring (10) is vertically arranged in a gap between the lifting plate (14) and the rotating plate (6), the upper end of the spring (10) abuts against the lower end face of the lifting plate (14), and the lower end of the spring (10) abuts against the upper end face of the rotating plate (6).
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| CN202010568724.1A CN111579306B (en) | 2020-06-20 | 2020-06-20 | Sampling device capable of reducing cell pollution probability in submission process |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010568724.1A CN111579306B (en) | 2020-06-20 | 2020-06-20 | Sampling device capable of reducing cell pollution probability in submission process |
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| CN111579306B true CN111579306B (en) | 2020-12-01 |
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| CN205719606U (en) * | 2016-06-24 | 2016-11-23 | 周赞虎 | Food test sampling box |
| CN209974782U (en) * | 2019-04-28 | 2020-01-21 | 新疆隆博叶希丽生态环保有限公司 | Be used for desert algae cell high density heterotrophic culture fermentation cylinder |
| CN210331172U (en) * | 2019-06-17 | 2020-04-17 | 常州市金坛区人民医院 | Infectious disease blood sample processing apparatus is used to infectious department |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110514488A (en) * | 2018-05-22 | 2019-11-29 | 佛山市嘉懿行农业科技有限公司 | A kind of sampler for biotechnology separation |
| US11137336B2 (en) * | 2018-10-31 | 2021-10-05 | Essen Instruments, Inc. | Integrated flow cytometer module and liquid handling system and methods for use |
| CN109880739A (en) * | 2019-03-17 | 2019-06-14 | 胥振国 | A kind of blood bacterium specimen sampling, culture apparatus and application method |
| CN211170664U (en) * | 2019-12-03 | 2020-08-04 | 河南侨创生命科技有限公司 | Umbilical cord mesenchymal stem cell combined type separation and extraction device |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4736636A (en) * | 1985-07-18 | 1988-04-12 | Dideco S.P.A. | Device for selectively sampling fluid from two sections of a line |
| CN205719606U (en) * | 2016-06-24 | 2016-11-23 | 周赞虎 | Food test sampling box |
| CN209974782U (en) * | 2019-04-28 | 2020-01-21 | 新疆隆博叶希丽生态环保有限公司 | Be used for desert algae cell high density heterotrophic culture fermentation cylinder |
| CN210331172U (en) * | 2019-06-17 | 2020-04-17 | 常州市金坛区人民医院 | Infectious disease blood sample processing apparatus is used to infectious department |
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Effective date of registration: 20201104 Address after: 266426 e-401, 58 Qinglonghe Road, Huangdao District, Qingdao City, Shandong Province Applicant after: Qingdao zhongdehua large cell technology Co., Ltd Address before: 310018 Zhejiang University of Technology, Hangzhou, Zhejiang Province, No. 18 Chao Wang Road Applicant before: Huang Lingfeng |
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