CN111575151A - Animal and plant cell culture machine - Google Patents

Animal and plant cell culture machine Download PDF

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Publication number
CN111575151A
CN111575151A CN202010456873.9A CN202010456873A CN111575151A CN 111575151 A CN111575151 A CN 111575151A CN 202010456873 A CN202010456873 A CN 202010456873A CN 111575151 A CN111575151 A CN 111575151A
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China
Prior art keywords
block
cell culture
plate
animal
plant cell
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CN202010456873.9A
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Chinese (zh)
Inventor
李小伟
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Beijing Tianfeng Minghua Hotel Management Co ltd
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Beijing Tianfeng Minghua Hotel Management Co ltd
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Priority to CN202010456873.9A priority Critical patent/CN111575151A/en
Publication of CN111575151A publication Critical patent/CN111575151A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M39/00Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms

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  • Wood Science & Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses an animal and plant cell culture machine, which comprises a machine body, a placing frame, a control panel, a support and a sealing door, wherein the control panel is electrically connected with the machine body, the support is arranged at the bottom of the machine body, the sealing door is hinged with the machine body, the placing frame is movably clamped with the inner wall of the machine body, when a lower pressing ring loses the pressure of a yeast cell culture medium, the control panel can swing downwards through the gravity of a swinging plate, so that a scraping block on the swinging plate can slide downwards, the scraping block can scrape the culture solution attached to the outer surface of the swinging plate, the yeast cell culture solution attached to a clearing block can be guided between two partition plates through the partition plates on the clearing block, so that a height difference is generated between the yeast cell culture solution and other cell culture mediums which are placed on the surface of the placing frame again, and the culture solution attached to the clearing block is effectively prevented from being difficult to clear, leading to contamination of other cell culture media.

Description

Animal and plant cell culture machine
Technical Field
The invention relates to the field of animal and plant cell culture, in particular to an animal and plant cell culture machine.
Background
The cell culture machine is mainly used for equipment for culturing animal and plant cells, can provide constant temperature and sterile environment for an animal and plant cell culture medium, so that cells in the cell culture medium can be stably propagated, and is a common equipment in the cell culture industry, and based on the above description, the inventor finds that the existing animal and plant cell culture machine mainly has the following defects, for example:
because the yeast cells can breathe during reproduction to produce a large amount of bubbles on the culture medium, if the culture medium placed in the cell culture machine holds too much culture solution, the bubbles that lead to the yeast cells respiration to produce easily overflow the culture medium and attach on the shelf of cell culture machine to when again cultivating different cells, the yeast cells attached on the shelf can cause the pollution to different cultured cells.
Disclosure of Invention
In order to solve the problems, the invention provides an animal and plant cell culture machine.
In order to achieve the purpose, the invention is realized by the following technical scheme: an animal and plant cell culture machine structurally comprises a machine body, a placing frame, a control panel, a support and a sealing door, wherein the control panel is electrically connected with the machine body; the placing frame comprises an outer frame and a touch prevention mechanism, wherein the touch prevention mechanism is fixedly embedded at the bottom of the inner wall of the outer frame.
As a further optimization of the invention, the anti-touch mechanism comprises a lower press ring, two swing plates and a bottom plate, wherein the lower press ring is movably clamped with the bottom plate, the swing plates are hinged with the bottom plate through the lower press ring, and the two swing plates are uniformly and symmetrically distributed on two sides of the bottom plate.
As a further optimization of the invention, the swing plate comprises a scraping block and a plate surface, the scraping block is movably clamped with the plate surface, and the outer surface of the scraping block is of an arc structure.
As a further optimization of the invention, the scraping block comprises a removing block, an absorbing block and a connecting block, the removing block and the connecting block are of an integrated structure, the absorbing block is embedded at the bottom of the removing block, and the absorbing block is made of polyester sponge with strong water absorption.
As a further optimization of the invention, the absorption block comprises a block body, through pipes and connecting pipes, the through pipes and the block body are of an integrated structure, the connecting pipes penetrate between the two through pipes, and the through pipes and the connecting pipes are both of hollow structures.
As a further optimization of the invention, the clearing block comprises four partition plates and four combination plates, wherein the partition plates and the combination plates are in an integrated structure, and the four partition plates are uniformly distributed on the upper surface of the combination plate in a parallel sheet mode.
As a further optimization of the invention, the partition plate comprises a liquid guide block, plate blocks and a connecting block, wherein the liquid guide block is fixedly embedded at the upper end of each plate block, the connecting block is arranged between every two adjacent plate blocks, and the liquid guide block is in a triangular structure.
As a further optimization of the invention, the liquid guide block comprises a liquid permeable block, a liquid storage tank and a plate body, the liquid permeable block is embedded and fixed at the top of the liquid storage tank, the liquid storage tank and the plate body are of an integrated structure, fourteen liquid storage tanks are arranged, and two liquid storage tanks are uniformly distributed on two sides of the plate body in a group and are symmetrical.
The invention has the following beneficial effects:
1. produce decurrent pressure to pushing down the clamp ring through placing the culture medium that prevents touching mechanism upper end, can make pushing down the clamp ring descend along the bottom plate, thereby make the swing board can expand it through the bottom plate both sides to produce ascending thrust, the institute makes the inside culture solution that overflows of yeast cell culture medium adhere to swing board upper surface, when the pressure ring loses the pressure of yeast cell culture medium down, can swing downwards through the gravity of swing board self, thereby make the piece of scraping on the swing board can slide down, thereby make the piece of scraping can scrape off the adnexed culture solution of swing board surface.
2. Can lead to between two baffles with the yeast cell culture solution that adheres to on cleaing away the piece through the baffle on the piece of cleaing away to make yeast cell culture solution and put into once more and produce the difference in height between the other cell culture mediums on the shelf surface, effectually avoided cleaing away the piece on adnexed culture solution difficult clear away, lead to causing the circumstances of pollution to other cell culture mediums.
Drawings
FIG. 1 is a schematic structural diagram of an animal and plant cell culture machine according to the present invention.
FIG. 2 is a schematic view of the partial front view of the interior of the shelf of the present invention.
Fig. 3 is a front view of the interior of the anti-touch mechanism of the present invention.
FIG. 4 is a front view of the interior of the swing plate of the present invention.
Fig. 5 is a front view of the interior of the scraping block of the present invention.
FIG. 6 is a front view of the interior of the absorbent block of the present invention.
FIG. 7 is a schematic diagram of a clearing block according to the present invention.
FIG. 8 is a schematic view of the structure of the separator of the present invention.
Fig. 9 is a front view of the inner structure of the liquid guide block of the present invention.
In the figure: the device comprises a machine body-1, a placing frame-2, a control panel-3, a support-4, a sealing door-5, an outer frame-21, an anti-touch mechanism-22, a pressing ring-a 1, a swinging plate-a 2, a bottom plate-a 3, a scraping block-a 21, a plate surface-a 22, a clearing block-b 1, an absorbing block-b 2, a connecting block-b 3, a block body-b 21, a through pipe-b 22, a connecting pipe-b 23, a partition plate-c 1, a combining plate-c 2, a liquid guide block-c 11, a plate-c 12, a connecting block-c 13, a liquid guide block-d 1, a liquid storage tank-d 2 and a plate body-d 3.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
As shown in fig. 1-6:
the invention provides an animal and plant cell culture machine, which structurally comprises a machine body 1, a placing frame 2, a control panel 3, a support 4 and a sealing door 5, wherein the control panel 3 is electrically connected with the machine body 1, the support 4 is arranged at the bottom of the machine body 1, the sealing door 5 is hinged with the machine body 1, and the placing frame 2 is movably clamped with the inner wall of the machine body 1; the placement frame 2 comprises an outer frame 21 and a touch prevention mechanism 22, wherein the touch prevention mechanism 22 is embedded and fixed at the bottom of the inner wall of the outer frame 21.
Wherein, it includes underpush ring a1, swing board a2, bottom plate a3 to prevent touching mechanism 22, underpush ring a1 and bottom plate a3 activity block, swing board a2 is through underpush ring a1 and bottom plate a3 hinged joint, swing board a2 is equipped with two, and even both sides at bottom plate a3 are the symmetric distribution, produce decurrent gravity to underpush ring a1 through the yeast cell culture medium of placing in the mechanism, can make underpush ring a1 descend along bottom plate a3 to make the avris 2 of bottom plate a3 both sides upwards promote by bottom plate a3 and strut.
The swing plate a2 comprises a scraping block a21 and a plate surface a22, the scraping block a21 is movably clamped with the plate surface a22, the outer surface of the scraping block a21 is of an arc-shaped structure, overflowing culture solution can slide on the outer surface of the scraping block a21 more easily, and the scraping block a21 can slide up and down along the outer surface of the plate surface a22 by the throwing force generated by the vertical swing of the plate surface a 22.
The scraping block a21 comprises a removing block b1, an absorbing block b2 and a connecting block b3, the removing block b1 and the connecting block b3 are of an integrated structure, the absorbing block b2 is embedded at the bottom of the removing block b1, and the absorbing block b2 is made of polyester sponge with strong water absorption and can absorb the culture solution remained after the object surface removing block b1 is scraped.
The absorption block b2 comprises a block b21, a penetration tube b22 and a connecting tube b23, the penetration tube b22 and the block b21 are of an integrated structure, the connecting tube b23 penetrates between the two penetration tubes b22, the penetration tube b22 and the connecting tube b23 are both hollow structures, the contact area between the block b21 and the outside can be increased, and therefore the air drying speed of the culture solution in the block b21 is increased.
The detailed use method and action of the embodiment are as follows:
in the present invention, since the yeast cells breathe during propagation to generate a large amount of bubbles in the culture medium, if the culture medium placed on the placement frame 2 contains too much culture medium, the bubbles generated by the respiration of the yeast cells are easily overflowed from the culture medium and attached to the placement frame 2, so that the yeast cells attached to the placement frame 2 pollute different cell culture mediums, and such problems can be effectively solved by the cooperation of the downward pressing ring a1 on the touch prevention mechanism 22 and the swinging plate a2, when the yeast culture medium is placed on the placement frame 2 in the body 1, the downward pressure is generated on the downward pressing ring a1 by the culture medium placed on the upper surface of the touch prevention mechanism 22, so that the downward pressing ring a1 can descend along the bottom plate a3, so that the swinging plate a2 can generate upward pushing force to expand through two sides of the bottom plate a3, and the culture medium overflowed from the interior of the yeast cells can be attached to the upper surface of the swinging plate a2, and the scraping block a21 can slide leftwards along the swinging plate a2 by the throwing force generated by the unfolding of the swinging plate a2, when the culture of the yeast cell culture medium is finished and taken out, the swinging plate a2 can swing downwards by the gravity of the swinging plate a2, so that the scraping block a21 on the swinging plate a2 can slide downwards along the gravity, so that the scraping block a21 can scrape the culture solution attached to the outer surface of the swinging plate a2, and the absorption block b2 on the scraping block a21 can absorb the culture solution remained on the outer surface of the swinging plate a2 after the scraping block a21 is scraped, so that the overflowing yeast cell culture solution can not directly contact other culture solution which is placed again to cause pollution, and the contact area of the absorption block b2 which is beneficial to the outside can be increased through the through tube b22 and the connecting tube b23 which are communicated with the outside on the absorption block b2, thereby accelerating the air drying speed of the culture solution in the absorption block b2, so that the absorbent block b2 can be used again.
Example 2
As shown in fig. 7-9:
the clearing block b1 comprises a partition plate c1 and a combination plate c2, the partition plate c1 and the combination plate c2 are integrated, the number of the partition plates c1 is four, the upper surfaces of the combination plates c2 are uniformly distributed in parallel, and overflowed yeast cell culture solution can be introduced between the two partition plates c1, so that height difference is generated between the overflowed yeast cell culture solution and other cell culture media, and the situation that yeast cells on the combination plate c2 enter other cell culture media is avoided.
The partition plate c1 includes a liquid guide block c11, a plate c12 and a connecting block c13, the liquid guide block c11 is embedded at the upper end of the plate c12, the connecting block c13 is installed between two adjacent plates c12, the liquid guide block c11 has a triangular structure, and the yeast cell culture liquid remaining on the upper surface of the plate c12 can be introduced into the connecting block c13 and then introduced into the mechanism through the connecting block c 13.
The liquid guide block c11 comprises a liquid permeable block d1, a liquid storage tank d2 and a plate body d3, the liquid permeable block d1 is embedded at the top of the liquid storage tank d2, the liquid storage tank d2 and the plate body d3 are of an integrated structure, the liquid permeable block d1 is made of textile fiber materials, has certain water permeability, and is slow in water permeability after being filled with water, the liquid storage tanks d2 are provided with fourteen liquid storage tanks, the fourteen liquid storage tanks and the two liquid storage tanks are uniformly distributed on two sides of the plate body d3 in a group mode, and when yeast cell culture liquid on the upper surface of the connecting block b3 is guided into the mechanism through the surface of the connecting block b3, a small amount of residual yeast cell culture liquid can be guided into the liquid storage tank d2 through the liquid permeable block d 1.
The detailed use method and action of the embodiment are as follows:
in the present invention, since the cleaning block b1 cannot clean the culture solution adhered to the outer surface of the swing plate a2 when scraping the culture solution adhered to the outer surface of the swing plate a2, the phenomenon that the culture solution adhered to the outer surface of the cleaning block b1 easily contacts with other culture solution rested on the shelf 2 can be effectively solved by the partition plate c1 on the cleaning block b1, the culture solution adhered to the outer surface of the cleaning block b1 can be introduced between the two partition plates c1 by the partition plate c1 on the cleaning block b1, so that a height difference is generated between the culture solution adhered to the outer surface of the cleaning block b1 and other cell culture medium rested on the surface of the shelf 2, and the culture solution adhered to the upper surface of the partition plate c1 can be introduced into the connecting block c13 by the liquid guide block c11 on the partition plate c1, so that the connecting block c13 can introduce the culture solution between the two partition plates c1, and a small amount of residual yeast cell culture solution can be guided into the liquid storage tank d2 through the liquid permeating block d1, so that the situation that the culture solution attached to the outer surface of the cleaning block b1 is not easy to clean and causes pollution due to contact with other cell culture media is effectively avoided.
The technical solutions of the present invention or similar technical solutions designed by those skilled in the art based on the teachings of the technical solutions of the present invention are all within the scope of the present invention to achieve the above technical effects.

Claims (8)

1. The utility model provides an animal and plant cell culture machine, its structure includes organism (1), places frame (2), control panel (3), support (4), sealing door (5), control panel (3) are connected with organism (1) electricity, the bottom position in organism (1) is installed in support (4), sealing door (5) and organism (1) hinged joint, its characterized in that: the placing frame (2) is movably clamped with the inner wall of the machine body (1);
the placement frame (2) comprises an outer frame (21) and a touch prevention mechanism (22), wherein the touch prevention mechanism (22) is embedded and fixed at the bottom of the inner wall of the outer frame (21).
2. An animal and plant cell culture machine according to claim 1, characterized in that: prevent touching mechanism (22) and include lower clamping ring (a1), swing board (a2), bottom plate (a3), lower clamping ring (a1) and bottom plate (a3) activity block, swing board (a2) are through lower clamping ring (a1) and bottom plate (a3) hinged joint.
3. An animal and plant cell culture machine according to claim 2, characterized in that: the swinging plate (a2) comprises a scraping block (a21) and a plate surface (a22), and the scraping block (a21) is movably clamped with the plate surface (a 22).
4. An animal and plant cell culture machine according to claim 3, characterized in that: the scraping block (a21) comprises a clearing block (b1), an absorbing block (b2) and a connecting block (b3), the clearing block (b1) and the connecting block (b3) are of an integrated structure, and the absorbing block (b2) is embedded at the bottom position of the clearing block (b 1).
5. An animal and plant cell culture machine according to claim 4, characterized in that: the absorption block (b2) comprises a block body (b21), a through pipe (b22) and a connecting pipe (b23), wherein the through pipe (b22) and the block body (b21) are of an integrated structure, and the connecting pipe (b23) penetrates between the two through pipes (b 22).
6. An animal and plant cell culture machine according to claim 4, characterized in that: the clearing block (b1) comprises a partition plate (c1) and a combination plate (c2), wherein the partition plate (c1) and the combination plate (c2) are of an integrated structure.
7. An animal and plant cell culture machine according to claim 6, characterized in that: the partition plate (c1) comprises a liquid guide block (c11), a plate block (c12) and a connecting block (c13), the liquid guide block (c11) is embedded at the upper end of the plate block (c12), and the connecting block (c13) is installed between two adjacent plate blocks (c 12).
8. An animal and plant cell culture machine according to claim 7, characterized in that: the liquid guide block (c11) comprises a liquid permeating block (d1), a liquid storage tank (d2) and a plate body (d3), the liquid permeating block (d1) is embedded at the top of the liquid storage tank (d2), and the liquid storage tank (d2) and the plate body (d3) are of an integrated structure.
CN202010456873.9A 2020-05-26 2020-05-26 Animal and plant cell culture machine Pending CN111575151A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010456873.9A CN111575151A (en) 2020-05-26 2020-05-26 Animal and plant cell culture machine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010456873.9A CN111575151A (en) 2020-05-26 2020-05-26 Animal and plant cell culture machine

Publications (1)

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CN111575151A true CN111575151A (en) 2020-08-25

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CN202010456873.9A Pending CN111575151A (en) 2020-05-26 2020-05-26 Animal and plant cell culture machine

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112006573A (en) * 2020-09-01 2020-12-01 黄亦堃 Multifunctional bathroom cabinet
CN112916073A (en) * 2021-02-05 2021-06-08 陈敏 A separating funnel frame for chemical industry
CN115011460A (en) * 2022-06-22 2022-09-06 徐群伟 Biochemical incubator for biological detection

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112006573A (en) * 2020-09-01 2020-12-01 黄亦堃 Multifunctional bathroom cabinet
CN112916073A (en) * 2021-02-05 2021-06-08 陈敏 A separating funnel frame for chemical industry
CN115011460A (en) * 2022-06-22 2022-09-06 徐群伟 Biochemical incubator for biological detection

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