CN111568791B - Anti-saccharification composition and preparation method and application thereof - Google Patents
Anti-saccharification composition and preparation method and application thereof Download PDFInfo
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- CN111568791B CN111568791B CN202010417452.5A CN202010417452A CN111568791B CN 111568791 B CN111568791 B CN 111568791B CN 202010417452 A CN202010417452 A CN 202010417452A CN 111568791 B CN111568791 B CN 111568791B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/37—Esters of carboxylic acids
- A61K8/375—Esters of carboxylic acids the alcohol moiety containing more than one hydroxy group
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/42—Amides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/55—Phosphorus compounds
- A61K8/553—Phospholipids, e.g. lecithin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9771—Ginkgophyta, e.g. Ginkgoaceae [Ginkgo family]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
Abstract
The invention discloses an anti-saccharification composition and a preparation method and application thereof, belonging to the field of cosmetics. According to the invention, the anoectochilus roxburghii extract, the ginkgo leaf extract, the hydroxytyrosol and the panthenol are compounded in a specific ratio, so that active ingredients such as flavone and polyphenol can better permeate into skin and better play a role in resisting saccharification; on the basis, hydroxytyrosol and panthenol are added to prepare the anti-saccharification composition into nano emulsion, so that the permeability of the effective components can be further enhanced; in addition, the nano emulsion is filtered by the mesoporous silica nano material, so that heavy metal, active oxygen and microorganisms in the nano emulsion can be effectively removed, the safety of the nano emulsion is improved, and the quality guarantee period of the nano emulsion is prolonged.
Description
Technical Field
The invention belongs to the field of cosmetics, and particularly relates to an anti-saccharification composition, and a preparation method and application thereof.
Background
The saccharification reaction means that redundant sugar is free in blood and attached to collagen to generate condensation, rearrangement and chain extension reactions to generate a high-reactivity saccharification intermediate product; further interaction with proteins leads to the formation of irreversible glycation end-products (AGEs). The damage of protein crosslinking and the accumulation of AGEs and their binding to specific receptors caused by glycation reaction cause a series of problems in skin, such as skin elasticity decrease, wrinkles, dryness, sagging, dark yellow and colored spots. At the same time, the skin renewal cycle is prolonged, and the metabolic function of skin regeneration is reduced. Anti-glycation is therefore required to allow the collagen of the skin to regain its physiological function, bringing the skin to a more youthful state. Therefore, there is a need for a cosmetic having an excellent anti-glycation effect.
Disclosure of Invention
In order to solve the disadvantages and shortcomings of the prior art, the present invention provides an anti-glycation composition, and a preparation method and application thereof.
In a first aspect, the invention provides an anti-glycation composition, which comprises the following components in parts by weight: 1-60 parts of anoectochilus roxburghii extract, 1-60 parts of ginkgo leaf extract, 1-10 parts of hydroxytyrosol and 0.1-30 parts of panthenol. The anoectochilus formosanus extract and the ginkgo leaf extract are rich in flavone and have good effect of removing free radicals; hydroxytyrosol is a natural polyphenol compound and has super-strong antioxidant whitening effect; panthenol is a precursor of vitamin B5, and can effectively permeate into stratum corneum to achieve the effect of moisturizing. The anti-glycation composition is prepared by compounding the anoectochilus roxburghii extract, the ginkgo leaf extract, hydroxytyrosol and panthenol at a specific ratio, so that flavone and polyphenol can better permeate into skin and better play the anti-glycation role.
As a preferred embodiment of the anti-glycation composition of the present invention, the anoectochilus formosanus extract and the ginkgo biloba leaf extract are both aqueous solutions.
As a preferred embodiment of the anti-glycation composition of the invention, the total flavone content in the anoectochilus formosanus extract is more than 10 wt%; the content of total flavonoids in the ginkgo leaf extract is more than 20 wt%; the purity of the hydroxytyrosol and the purity of the panthenol are respectively more than 99.0 wt%.
As a preferred embodiment of the anti-glycation composition of the present invention, the anti-glycation composition nanoemulsion further comprises the following components in parts by weight: 0.1-5 parts of lecithin and 5-20 parts of caprylic/capric triglyceride. By adding lecithin and caprylic/capric triglyceride, the anti-saccharification composition can be prepared into nano emulsion to increase the permeability of the effective components and better exert the anti-saccharification effect.
As a preferred embodiment of the anti-glycation composition of the present invention, the anti-glycation composition comprises the following components in parts by weight: 38 parts of anoectochilus roxburghii extract, 36 parts of ginkgo leaf extract, 6 parts of hydroxytyrosol, 20 parts of panthenol, 3 parts of lecithin and 15 parts of caprylic/capric triglyceride.
In a second aspect, the present invention provides a method for preparing the above anti-glycation composition, which comprises the steps of:
(1) uniformly mixing anoectochilus formosanus extract, ginkgo leaf extract, hydroxytyrosol and panthenol to obtain a mixed solution;
(2) adding lecithin into the mixed solution obtained in the step (1), uniformly mixing, adding caprylic/capric triglyceride, performing dispersion pretreatment to obtain primary emulsion, and performing high-pressure homogenization treatment on the primary emulsion to obtain nano emulsion;
(3) and (3) filtering the nano emulsion obtained in the step (2) through a silicon dioxide nano material film to obtain the anti-saccharification composition, wherein the silicon dioxide nano material film is mainly prepared from N-carboxymethyl chitosan and a mesoporous silicon dioxide nano material.
The plant active ingredients are inevitably brought into metal ions during extraction, and a large amount of active oxygen exists in the plant extract, and the active oxygen is generated mainly due to the following aspects: on the first hand, the plant cell in the plant extract generates a large amount of active oxygen due to the leakage of electrons in the electron transfer process at all times on chloroplasts, mitochondria and plasma membranes during the life process of the plant cell, and on the second hand, some molecular oxygen (O) enters into the plant body2) The molecular oxygen can be reduced by a single electron to be converted into superoxide anion free radical, and can be derived into active oxygen such as hydroxyl free radical, singlet oxygen, hydrogen peroxide, lipid peroxide free radical and the like, and the generated active oxygen can cause skin tissue degeneration and edema, hyperemia, epidermal hyperplasia and cuticle formation, reduce the water entering into the skin, accelerate the skin drying, increase the pigment precipitation and accelerate the skin aging. The silicon dioxide nano material film is mainly prepared from N-carboxymethyl chitosan and a mesoporous silicon dioxide nano material, wherein the N-carboxymethyl chitosan is an amphoteric polyelectrolyte, can efficiently adsorb heavy metal ions with positive charges, and has the effect of resisting active oxygen, so that the skin can be protected from being damaged by the active oxygen; will be negatively chargedThe mesoporous silica nano material is embedded into the N-carboxymethyl chitosan membrane, so that the adsorption effect of the filtering membrane on heavy metals can be greatly improved, meanwhile, the scavenging effect of the N-carboxymethyl chitosan membrane on active oxygen can be improved, and the safety of the anti-saccharification composition can be further improved. In addition, the self-made filter membrane can be used for filtering microorganisms in the anti-saccharification composition, and the shelf life of the anti-saccharification composition is effectively prolonged.
The preparation method is characterized in that the dispersion treatment is carried out before the high-pressure homogenization, so that the stability of the obtained nano emulsion is improved.
As a preferred embodiment of the preparation method of the present invention, the preparation method of the silica nanomaterial membrane comprises the steps of:
A. dissolving N-carboxymethyl chitosan in water, and uniformly stirring to obtain an N-carboxymethyl chitosan solution;
B. and (3) adding the mesoporous silica nano material into the N-carboxymethyl chitosan solution obtained in the step (A), uniformly stirring, drying and forming a film to obtain the silica nano material film.
In a preferred embodiment of the preparation method of the present invention, the mesoporous silica nanomaterial is a cage-type mesoporous silica nanomaterial.
In a preferred embodiment of the preparation method of the present invention, the mass concentration of the N-carboxymethyl chitosan solution is 1 to 5%.
In a preferred embodiment of the preparation method of the present invention, in the step B, the solid-to-liquid ratio of the mesoporous silica nanomaterial to the N-carboxymethyl chitosan solution is 1g:5 mL.
As a preferred embodiment of the preparation method of the present invention, the dispersion pretreatment is performed at a rotation speed of 12000r/min 5000-.
As a preferred embodiment of the preparation method of the present invention, the time of the dispersion pretreatment is 3 to 6 min.
As a preferred embodiment of the preparation method of the present invention, the conditions of the high-pressure homogenization treatment are as follows: the pressure is 500-1200bar/min, the temperature is 35-45 ℃, and the cycle time is 6-10 times.
In a third aspect, the present invention provides the use of an anti-glycation composition as described above in cosmetics.
Compared with the prior art, the invention has the following advantages: according to the anti-saccharification composition, the anoectochilus roxburghii extract, the ginkgo leaf extract, the hydroxytyrosol and the panthenol are compounded in a specific ratio, so that the flavone and the polyphenol can better permeate into the skin and can better play the anti-saccharification effect; the anti-saccharification composition is prepared into the nano emulsion by adding hydroxytyrosol and panthenol so as to further enhance the permeability of active ingredients and better play the anti-saccharification effect, and the nano emulsion is filtered by a self-made mesoporous silica nano material, so that heavy metals, active oxygen and microorganisms in the nano emulsion can be effectively removed, the safety of the nano emulsion is improved, and the quality guarantee period of the nano emulsion is prolonged.
Drawings
FIG. 1 is a graph showing the results of the CCK-8 assay in effect example 2 in which AGEs influence the induction of apoptosis of HDF (human dermal fibroblast) by the anti-glycation composition;
FIG. 2 is a graph showing the results of permeation of total flavonoids in the anti-glycation composition measured by Franz diffusion cell method in Effect example 3.
Detailed Description
The invention is further illustrated by the following examples in order to better illustrate the objects, aspects and advantages of the invention. It is apparent that the following examples are only a part of the embodiments of the present invention, and not all of them. It should be understood that the embodiments of the present invention are only for illustrating the technical effects of the present invention, and are not intended to limit the scope of the present invention.
Example 1
This example is an embodiment of the anti-glycation composition of the present invention, which is a nanoemulsion comprising the following components in parts by weight: 38 parts of anoectochilus roxburghii extract, 36 parts of ginkgo leaf extract, 6 parts of hydroxytyrosol, 20 parts of panthenol, 3 parts of lecithin and 15 parts of caprylic/capric triglyceride. The method for preparing the anti-glycation composition comprises the following steps:
(1) uniformly mixing anoectochilus formosanus extract, ginkgo leaf extract, hydroxytyrosol and panthenol to obtain a mixed solution;
(2) adding lecithin into the mixed solution obtained in the step (1), uniformly mixing, adding caprylic acid/capric acid triglyceride, dispersing for 5min under the condition of 8000r/min to obtain primary emulsion, and carrying out high-pressure homogenization treatment on the primary emulsion to obtain nano emulsion, wherein the high-pressure homogenization condition is that the pressure is 700bar/min, the temperature is 35-45 ℃, and the cycle time is 8 times;
(3) filtering the nano emulsion obtained in the step (2) by a silicon dioxide nano material film to obtain the anti-saccharification composition, wherein the preparation method of the silicon dioxide nano material comprises the following steps:
A. dissolving N-carboxymethyl chitosan in water, and uniformly stirring to obtain an N-carboxymethyl chitosan solution with the mass concentration of 3%;
B. and (3) adding the mesoporous silica nano material into the N-carboxymethyl chitosan solution obtained in the step (A) according to the solid-to-liquid ratio of 1g to 5mL, uniformly stirring, drying, and forming a film to obtain the silica nano material film.
Example 2
This example is an embodiment of the anti-glycation composition of the present invention, which is a nanoemulsion comprising the following components in parts by weight: 60 parts of anoectochilus roxburghii extract, 60 parts of ginkgo leaf extract, 10 parts of hydroxytyrosol, 30 parts of panthenol, 5 parts of lecithin and 20 parts of caprylic/capric triglyceride. The method for preparing the anti-glycation composition comprises the following steps:
(1) uniformly mixing anoectochilus formosanus extract, ginkgo leaf extract, hydroxytyrosol and panthenol to obtain a mixed solution;
(2) adding lecithin into the mixed solution obtained in the step (1), uniformly mixing, adding caprylic acid/capric acid triglyceride, dispersing for 6min under the condition of 12000r/min to obtain primary emulsion, and performing high-pressure homogenization treatment on the primary emulsion to obtain nano emulsion, wherein the high-pressure homogenization condition is that the pressure is 1200bar/min, the temperature is 35-45 ℃, and the cycle time is 10 times;
(3) filtering the nano emulsion obtained in the step (2) by a silicon dioxide nano material film to obtain the anti-saccharification composition, wherein the preparation method of the silicon dioxide nano material comprises the following steps:
A. dissolving N-carboxymethyl chitosan in water, and uniformly stirring to obtain an N-carboxymethyl chitosan solution with the mass concentration of 3%;
B. and (3) adding the mesoporous silica nano material into the N-carboxymethyl chitosan solution obtained in the step (A) according to the solid-to-liquid ratio of 1g to 5mL, uniformly stirring, drying, and forming a film to obtain the silica nano material film.
Example 3
This example is an embodiment of the anti-glycation composition of the present invention, which is a nanoemulsion comprising the following components in parts by weight: 1 part of anoectochilus roxburghii extract, 1 part of ginkgo leaf extract, 1 part of hydroxytyrosol, 0.1 part of panthenol, 0.1 part of lecithin and 5 parts of caprylic/capric triglyceride. The method for preparing the anti-glycation composition comprises the following steps:
(1) uniformly mixing anoectochilus formosanus extract, ginkgo leaf extract, hydroxytyrosol and panthenol to obtain a mixed solution;
(2) adding lecithin into the mixed solution obtained in the step (1), uniformly mixing, adding caprylic acid/capric acid triglyceride, dispersing for 3min under the condition of 5000r/min to obtain primary emulsion, and performing high-pressure homogenization treatment on the primary emulsion to obtain nano emulsion, wherein the high-pressure homogenization condition is that the pressure is 500bar/min, the temperature is 35-45 ℃, and the cycle time is 6 times;
(3) filtering the nano emulsion obtained in the step (2) by a silicon dioxide nano material film to obtain the anti-saccharification composition, wherein the preparation method of the silicon dioxide nano material comprises the following steps:
A. dissolving N-carboxymethyl chitosan in water, and uniformly stirring to obtain an N-carboxymethyl chitosan solution with the mass concentration of 3%;
B. and (3) adding the mesoporous silica nano material into the N-carboxymethyl chitosan solution obtained in the step (A) according to the solid-to-liquid ratio of 1g to 5mL, uniformly stirring, drying, and forming a film to obtain the silica nano material film.
Example 4
This example is an embodiment of the anti-glycation composition of the present invention, which is a nanoemulsion comprising the following components in parts by weight: 38 parts of anoectochilus roxburghii extract, 36 parts of ginkgo leaf extract, 6 parts of hydroxytyrosol, 20 parts of panthenol, 4 parts of lecithin and 18 parts of caprylic/capric triglyceride. The method for preparing the anti-glycation composition comprises the following steps:
(1) uniformly mixing anoectochilus formosanus extract, ginkgo leaf extract, hydroxytyrosol and panthenol to obtain a mixed solution;
(2) adding lecithin into the mixed solution obtained in the step (1), uniformly mixing, adding caprylic acid/capric acid triglyceride, dispersing for 5min under the condition of 8000r/min to obtain primary emulsion, and performing high-pressure homogenization treatment on the primary emulsion to obtain nano emulsion, wherein the high-pressure homogenization condition is that the pressure is 500bar/min, the temperature is 35-45 ℃, and the cycle time is 6 times;
(3) filtering the nano emulsion obtained in the step (2) by a silicon dioxide nano material film to obtain the anti-saccharification composition, wherein the preparation method of the silicon dioxide nano material comprises the following steps:
A. dissolving N-carboxymethyl chitosan in water, and uniformly stirring to obtain an N-carboxymethyl chitosan solution with the mass concentration of 5%;
B. and (3) adding the mesoporous silica nano material into the N-carboxymethyl chitosan solution obtained in the step (A) according to the solid-to-liquid ratio of 1g to 5mL, uniformly stirring, drying, and forming a film to obtain the silica nano material film.
Example 5
This example is an embodiment of the anti-glycation composition of the present invention, which is a nanoemulsion comprising the following components in parts by weight: 30 parts of anoectochilus roxburghii extract, 30 parts of ginkgo leaf extract, 10 parts of hydroxytyrosol, 30 parts of panthenol, 2 parts of lecithin and 10 parts of caprylic/capric triglyceride. The method for preparing the anti-glycation composition comprises the following steps:
(1) uniformly mixing anoectochilus formosanus extract, ginkgo leaf extract, hydroxytyrosol and panthenol to obtain a mixed solution;
(2) adding lecithin into the mixed solution obtained in the step (1), uniformly mixing, adding caprylic acid/capric acid triglyceride, dispersing for 5min under the condition of 8000r/min to obtain primary emulsion, and carrying out high-pressure homogenization treatment on the primary emulsion to obtain nano emulsion, wherein the high-pressure homogenization condition is that the pressure is 700bar/min, the temperature is 35-45 ℃, and the cycle time is 8 times;
(3) filtering the nano emulsion obtained in the step (2) by a silicon dioxide nano material film to obtain the anti-saccharification composition, wherein the preparation method of the silicon dioxide nano material comprises the following steps:
A. dissolving N-carboxymethyl chitosan in water, and uniformly stirring to obtain an N-carboxymethyl chitosan solution with the mass concentration of 3%;
B. and (3) adding the mesoporous silica nano material into the N-carboxymethyl chitosan solution obtained in the step (A) according to the solid-to-liquid ratio of 1g to 5mL, uniformly stirring, drying, and forming a film to obtain the silica nano material film.
Comparative example 1
The comparative example relates to an anti-saccharification composition, which is a nano emulsion and comprises the following components in parts by weight: 0.1 part of anoectochilus roxburghii extract, 70 parts of ginkgo leaf extract, 20 parts of hydroxytyrosol, 9.9 parts of panthenol, 6 parts of lecithin and 25 parts of caprylic/capric triglyceride. This comparative example anti-glycation composition was prepared in the same manner as in example 1.
Comparative example 2
The comparative example relates to an anti-saccharification composition, which is a nano emulsion and comprises the following components in parts by weight: 70 parts of anoectochilus roxburghii extract, 0.1 part of ginkgo leaf extract, 20 parts of hydroxytyrosol, 9.9 parts of panthenol, 3 parts of lecithin and 18 parts of caprylic/capric triglyceride. This comparative example anti-glycation composition was prepared in the same manner as in example 1.
Comparative example 3
The comparative example relates to an anti-saccharification composition, which is a nano emulsion and comprises the following components in parts by weight: 38 parts of anoectochilus roxburghii extract, 36 parts of ginkgo leaf extract, 6 parts of hydroxytyrosol, 0 part of panthenol, 3 parts of lecithin and 15 parts of caprylic/capric triglyceride. This comparative example anti-glycation composition was prepared in the same manner as in example 1.
Comparative example 4
The comparative example relates to an anti-saccharification composition, which is a nano emulsion and comprises the following components in parts by weight: 38 parts of anoectochilus roxburghii extract, 36 parts of ginkgo leaf extract, 6 parts of hydroxytyrosol, 20 parts of panthenol, 3 parts of lecithin and 15 parts of caprylic/capric triglyceride. This comparative example was prepared in the same manner as in example 1 except that no dispersion pretreatment was performed in step (2).
Comparative example 5
The comparative example relates to an anti-saccharification composition, which is a nano emulsion and comprises the following components in parts by weight: 38 parts of anoectochilus roxburghii extract, 36 parts of ginkgo leaf extract, 6 parts of hydroxytyrosol, 20 parts of panthenol, 3 parts of lecithin and 15 parts of caprylic/capric triglyceride. This comparative example was prepared in the same manner as in example 1 except that filtration of the silica nanomaterial membrane was not carried out.
Comparative example 6
The comparative example relates to an anti-saccharification composition, which is a nano emulsion and comprises the following components in parts by weight: 38 parts of anoectochilus roxburghii extract, 36 parts of ginkgo leaf extract, 6 parts of hydroxytyrosol, 20 parts of panthenol, 3 parts of lecithin and 15 parts of monoglyceride. This comparative example an anti-glycation composition was prepared as in example 1 except that the caprylic/capric triglyceride was replaced with the monoglyceride.
Comparative example 7
This comparative example relates to an anti-glycation composition, which is a nanoemulsion and is the same as example 1 except that it does not contain the anoectochilus formosanus extract. This comparative example an anti-glycation composition was prepared as in example 1 except that no Anoectochilus formosanus extract was used.
Comparative example 8
This comparative example relates to an anti-glycation composition, which is a nanoemulsion and is the same as example 1 except that it does not contain ginkgo biloba extract. This comparative example was prepared in the same manner as in example 1 except that the extract of ginkgo biloba leaves was not used.
Comparative example 9
This comparative example relates to an anti-glycation composition which is a nanoemulsion and is the same as example 1 except that it does not contain hydroxytyrosol. This comparative example anti-glycation composition was prepared as in example 1 except that hydroxytyrosol was not used.
In each of the examples and comparative examples, lecithin and caprylic/capric triglyceride were the same; the anoectochilus formosanus extract is water solution with total flavone content above 10 wt%; the folium Ginkgo extract is water solution with total flavone content of above 20 wt%; the hydroxytyrosol is the same and has the purity of more than 99.0 wt%; panthenol is also the same and has a purity of 99.0 wt% or more.
Effect example 1: stability test
Test samples: anti-glycation compositions prepared in examples 1-5 and comparative examples 1-6.
The test method comprises the following steps: placing the prepared anti-saccharification composition into a high-speed centrifuge, centrifuging for 5min, 10min, 15min and 20min at 25 ℃ and 12000r/min respectively, observing whether a sample is layered, detecting particle size change and dispersion index (PDI), and calculating a centrifugation stability parameter KE.
Wherein the centrifugal stability parameter KE is calculated as follows:
wherein, S0 is the average particle size of cosmetic acid before centrifugation; and S is the average grain size of the cosmetic acid after centrifugation.
Table 1 stability test results
As can be seen from table 1, as the centrifugation time is prolonged, the particle size of the anti-saccharification composition provided by the present invention is not changed much, and PDI is smaller and smaller, which indicates that the centrifugation does not affect the particle size of the anti-saccharification composition, but rather the active ingredient is dispersed more uniformly, and the centrifugation does not generate precipitation, which causes oil-water separation. Example 1 the particle size change during centrifugation was minimal and is the best embodiment of the present invention. Comparative examples 4, 5 differ from example 1 in that comparative examples 4, 5 do not respectively have a pre-emulsification treatment (i.e., dispersion pretreatment) before the high-pressure homogenization of the composition, and a silica membrane filtration, illustrating the effects of the pre-emulsification treatment and the silica membrane filtration to stabilize the composition structure. Example 1 compared to comparative example 6, the anti-glycation composition of the present invention using caprylic/capric triglyceride has better stability.
Effect example 2: composition in vitro anti-glycation assay
The anti-saccharification effect of the sample is tested by detecting apoptosis by a CCK-8 method, which specifically comprises the following steps: (1) the primary skin fibroblasts are cultured in DMEM medium containing 15% fetal calf serum, 100U/mL penicillin and 100U/mL streptomycin at 37 ℃ and 5% CO2Culturing in an incubator, changing culture solution for 1-2 days, and carrying out passage when the cells grow to 90% fusion; (2) taking primary skin fibroblast cells in logarithmic growth phase at 1 × 104The density per well was inoculated in a 96-well plate, and after 24 hours of culture, 100. mu.L of each stimulation sample was added to each group as shown in Table 2, and 200. mu.L of DMEM medium containing 15% fetal bovine serum was added thereto. Each group is provided with 5 multiple wells, after 24, 48 and 72 hours of culture, each well is added with 20 microliter of CCK-8 liquid, the cells are placed back into the cell incubator for 1 hour of incubation, and an enzyme linked immunosorbent assay detector is used for detecting the absorbance value of each well at the wavelength of 450 nm. The experiment was repeated 3 times and the results are shown in figure 1.
Table 2 respective groups of corresponding samples
| Group of | Adding stimulation samples |
| Stimulation group 1 | 300μg/mL |
| Stimulation group | |
| 2 | 300. mu.g/mL AGEs +1mmol/L of the anti-glycation composition of example 1 |
| |
300. mu.g/mL AGEs +1mmol/L of the anti-glycation composition of example 2 |
| |
300. mu.g/mL AGEs +1mmol/L example 3 anti-glycation composition |
| Stimulation group 5 | 300. mu.g/mL AGEs +1mmol/L example 4 anti-glycation composition |
| Stimulation group 6 | 300. mu.g/mL AGEs +1mmol/L example 5 anti-glycation composition |
| Stimulation group 7 | 300 μ g/mL AGEs +1mmol/L comparative example 1 anti-glycation |
| Stimulation group | |
| 8 | 300 μ g/mL AGEs +1mmol/L comparative example 2 anti-glycation composition |
| Stimulation group 9 | 300 μ g/mL AGEs +1mmol/L COMPARATIVE EXAMPLE 3 Antiglycation |
| Stimulation group | |
| 10 | 300μgAGEs +1mmol/L COMPARATIVE EXAMPLE 4 AntiGLYCERING COMPOSITIONS |
| Stimulation group 11 | 300 μ g/mL AGEs +1mmol/L COMPARATIVE EXAMPLE 5 Antiglycation compositions |
| Stimulation group 12 | 300 μ g/mL AGEs +1mmol/L COMPARATIVE EXAMPLE 6 AntiGLYCOSYLATING COMPOSITION |
| Stimulation group 13 | 300 μ g/mL AGEs +1mmol/L COMPARATIVE EXAMPLE 7 AntiGLYCOSYLATING |
| Stimulation group | |
| 14 | 300 μ g/mL AGEs +1mmol/L COMPARATIVE EXAMPLE 8 AntiGLYCOSYLIC COMPOSITIONS |
| Stimulation group 15 | 300 μ g/mL AGEs +1mmol/L COMPARATIVE EXAMPLE 9 AntiGLYCOSYLIC COMPOSITION |
| Control group | 300μg/mL BSA |
| Blank group | Is free of |
As can be seen from fig. 1, the anti-sugar composition prepared under the conditions of example 1 had a good effect of inhibiting apoptosis by AGEs, compared to the comparative example in which panthenol was absent, and high-pressure homogeneous pretreatment or filtration of a silica material membrane was not performed.
Effect example 3: absorption test of composition
By the Franz diffusion cell method, 0.1g of the anti-glycation composition (example 1, one of comparative examples 1 to 9) was uniformly applied to the area of the rat skin supplied to the diffusion cell, and each group was paralleled 3 times, 1mL of the receiving solution was sucked at 1.0, 2.0, 4.0, 6.0, 8.0, 10.0, and 12.0 hours, and the same volume of the receiving solution was filled after each sampling, and the total flavone content was measured and the transmittance was calculated. The results are shown in FIG. 2.
As shown in fig. 2, the cumulative permeation amount of example 1 was more effective than that of the anti-glycation composition lacking panthenol, indicating that panthenol has an effect of promoting percutaneous absorption of the composition.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (8)
1. An anti-saccharification composition is characterized by comprising the following components in parts by weight: 1-60 parts of anoectochilus roxburghii extract, 1-60 parts of ginkgo leaf extract, 1-10 parts of hydroxytyrosol, 0.1-30 parts of panthenol, 0.1-5 parts of lecithin and 5-20 parts of caprylic/capric triglyceride; the anoectochilus formosanus extract and the ginkgo leaf extract are both aqueous solutions; the preparation method of the anti-saccharification composition comprises the following steps:
(1) uniformly mixing anoectochilus formosanus extract, ginkgo leaf extract, hydroxytyrosol and panthenol to obtain a mixed solution;
(2) adding lecithin into the mixed solution obtained in the step (1), uniformly mixing, adding caprylic/capric triglyceride, performing dispersion pretreatment to obtain primary emulsion, and performing high-pressure homogenization treatment on the primary emulsion to obtain nano emulsion;
(3) and (3) filtering the nano emulsion obtained in the step (2) through a silicon dioxide nano material film to obtain the anti-saccharification composition, wherein the silicon dioxide nano material film is mainly prepared from N-carboxymethyl chitosan and a mesoporous silicon dioxide nano material.
2. The anti-glycation composition according to claim 1, wherein the total flavone content in the anoectochilus formosanus extract is 10 wt% or more; the content of total flavonoids in the ginkgo leaf extract is more than 20 wt%; the purity of the hydroxytyrosol and the purity of the panthenol are respectively more than 99.0 wt%.
3. The anti-glycation composition according to claim 1, characterized in that the anti-glycation composition comprises the following components in parts by weight: 38 parts of anoectochilus roxburghii extract, 36 parts of ginkgo leaf extract, 6 parts of hydroxytyrosol, 20 parts of panthenol, 3 parts of lecithin and 15 parts of caprylic/capric triglyceride.
4. A method for preparing an anti-glycation composition according to any one of claims 1-3, characterized in that the method comprises the steps of:
(1) uniformly mixing anoectochilus formosanus extract, ginkgo leaf extract, hydroxytyrosol and panthenol to obtain a mixed solution;
(2) adding lecithin into the mixed solution obtained in the step (1), uniformly mixing, adding caprylic/capric triglyceride, performing dispersion pretreatment to obtain primary emulsion, and performing high-pressure homogenization treatment on the primary emulsion to obtain nano emulsion;
(3) and (3) filtering the nano emulsion obtained in the step (2) through a silicon dioxide nano material film to obtain the anti-saccharification composition, wherein the silicon dioxide nano material film is mainly prepared from N-carboxymethyl chitosan and a mesoporous silicon dioxide nano material.
5. The method as claimed in claim 4, wherein the dispersion pretreatment is carried out at a rotation speed of 12000r/min of 5000-.
6. The method according to claim 4, wherein the time for the dispersion pretreatment is 3 to 6 min.
7. The method according to claim 4, wherein the conditions of the high-pressure homogenization treatment are as follows: the pressure is 500-1200bar/min, the temperature is 35-45 ℃, and the cycle time is 6-10 times; the preparation method of the silicon dioxide nano material film comprises the following steps:
A. dissolving N-carboxymethyl chitosan in water, and uniformly stirring to obtain an N-carboxymethyl chitosan solution;
B. and (3) adding the mesoporous silica nano material into the N-carboxymethyl chitosan solution obtained in the step (A), uniformly stirring, drying and forming a film to obtain the silica nano material film.
8. Use of the anti-glycation composition according to any one of claims 1-3 in cosmetics.
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