CN111559564A

CN111559564A - Biological reagent container for gene sequencing

Info

Publication number: CN111559564A
Application number: CN202010501341.2A
Authority: CN
Inventors: 张志峰; 张学强
Original assignee: Chengdu Wanzhong One Core Biotechnology Co ltd
Current assignee: Chengdu Wanzhong One Core Biotechnology Co ltd
Priority date: 2020-06-04
Filing date: 2020-06-04
Publication date: 2020-08-21

Abstract

The invention discloses a biological reagent container for gene sequencing, and belongs to the technical field of biochemical detection. The biological reagent container includes: the box body is provided with an opening at one end along a first direction; the reagent accommodating part comprises an accommodating body and a positioning body arranged at one end of the accommodating body, and the accommodating body is arranged in the box body; the cover plate assembly is covered at the opening; the cover plate assembly comprises a first cover plate and a second cover plate which are sequentially arranged along the direction far away from the box body, and the second cover plate is used for pressing the positioning body on the first cover plate; a first sealing element is arranged between the first cover plate and the box body; and a second sealing element is arranged between the positioning body and the first cover plate. According to the invention, through the arrangement of the first sealing element and the second sealing element and the pressing effect of the cover plate, good sealing between the reagent accommodating element and the box body is realized.

Description

Biological reagent container for gene sequencing

Technical Field

The invention relates to the technical field of biochemical detection, in particular to a biological reagent container for gene sequencing.

Background

Gene sequencing is the detection of nucleic acid sequences of living organisms based on the base complementary pairing principle. In order to facilitate the uniform management of reagents, in the gene sequencing process, the reagent tubes for gene sequencing are generally uniformly arranged in a reagent kit, and then the whole reagent kit is moved and loaded into a gene detection instrument to complete detection. However, the existing reagent kit has poor stability of placing reagent tubes, and the reagent tubes are easy to shake and collide during moving; secondly, the existing reagent box has poor sealing performance, if the reagent leaks, the reagent can enter the reagent box and pollute the whole reagent box, the reagent box is scrapped, and meanwhile, the leaked reagent can be doped in the reagent box, so that the detection accuracy is influenced.

Therefore, it is desirable to provide a biological reagent container for use in gene sequencing to solve the above problems.

Disclosure of Invention

The invention aims to provide a biological reagent container for gene sequencing, which can stably place a reagent accommodating part in a box body and realize the stable transfer and loading of the biological reagent container; meanwhile, the biological reagent container has good sealing performance, and can effectively avoid the pollution of the biological reagent container and the doping of reagents.

In order to realize the purpose, the following technical scheme is provided:

a biological reagent container for use in gene sequencing, comprising:

the box body is provided with an opening at one end along a first direction;

the reagent accommodating part comprises an accommodating body and a positioning body arranged at one end of the accommodating body, and the accommodating body is arranged in the box body;

the cover plate assembly is covered at the opening; the cover plate assembly comprises a first cover plate and a second cover plate which are sequentially arranged along the direction far away from the box body, and the second cover plate is used for pressing the positioning body on the first cover plate;

a first sealing element is arranged between the first cover plate and the box body; and a second sealing element is arranged between the positioning body and the first cover plate.

Preferably, the first cover plate is connected with the box body through a fastener, and the fastener penetrates through the first cover plate and the first sealing element in sequence and then is locked on the box body; a third seal is disposed between the fastener and the first cover plate.

Preferably, the positioning body is of a T-shaped structure and comprises a connecting portion and an abutting portion, the connecting portion is connected with the accommodating body, and the abutting portion abuts against the first cover plate and the second cover plate respectively along two end faces of the first direction.

Preferably, the bottom of the second cover plate is provided with a yielding groove, and the abutting part is accommodated in the yielding groove and abuts against the groove top of the yielding groove.

Preferably, a first through hole is formed in the first cover plate, and the accommodating body of the reagent accommodating part enters the box body from the first through hole; the periphery of the first through hole is provided with a first sealing groove, and the first sealing element is arranged in the first sealing groove and is simultaneously abutted against the groove bottom of the first sealing groove and the bottom surface of the positioning body.

Preferably, the second cover plate is connected with the box body through a snap assembly, so that the second cover plate presses the sealing member.

Preferably, the biological reagent container is a square structure, and at least one buckle component is arranged on each side of the square structure.

Preferably, the positioning body is provided with an opening to communicate the accommodating body with the outside; the reagent holding member further comprises a first blocking member for blocking the opening;

and a second through hole is formed in the second cover plate, and the first plugging piece is exposed out of the second through hole.

Preferably, the other end of the box body along the first direction is closed, and a handle is arranged on the box body; still set up first holding tank on the terminal surface of the blind end of box body, the handle can be retrieved in the first holding tank.

Preferably, the box body further comprises a supporting body, the supporting body is arranged in the box body, a second accommodating groove is formed in the supporting body, and the accommodating body is arranged in the second accommodating groove.

Preferably, the number of reagent holding members is at least two.

Preferably, the reagent holding member is a flexible bag; the first cover plate is provided with a vent hole which is communicated with the box body; and a second plugging piece is arranged at the vent hole.

Compared with the prior art, the invention has the beneficial effects that:

according to the invention, the cover plate component is pressed on the positioning body to realize the assembly of the reagent accommodating part and the box body, so that the stable placement of the reagent accommodating part is ensured, the mutual collision caused by shaking of the reagent accommodating part is avoided, and the stable transfer and loading of a biological reagent container are further facilitated; simultaneously through the setting of first sealing member and second sealing member, combine the pressure of apron to establish the effect, realized that reagent holds the good sealing between piece and the box body, not only can avoid reagent to leak and cause the pollution to biological reagent container, can also avoid the doping between the reagent to the influence of testing result accuracy.

Drawings

FIG. 1 is a schematic diagram of a biological reagent container for use in gene sequencing according to an embodiment of the present invention, shown from a first perspective;

FIG. 2 is an exploded view of a biological reagent container for use in gene sequencing according to an embodiment of the present invention;

FIG. 3 is an exploded view of a biological reagent container for use in gene sequencing according to an embodiment of the present invention with the second cover removed;

FIG. 4 is a first cross-sectional view of a biological reagent container for use in gene sequencing according to an embodiment of the present invention;

FIG. 5 is a second cross-sectional view of a biological reagent container for use in gene sequencing in an embodiment of the invention;

FIG. 6 is a cross-sectional view of a reagent holding member in an embodiment of the present invention;

FIG. 7 is a schematic view of a buckle assembly disposed on a box portion according to an embodiment of the present invention;

FIG. 8 is a cross-sectional view of another reagent holding member in an embodiment of the invention;

FIG. 9 is a schematic diagram of a biological reagent container for gene sequencing according to an embodiment of the present invention, shown from a second perspective.

Reference numerals:

10-a box body; 20-a cover plate assembly; 30-a reagent holding member; 40-a buckle assembly; 50-a first seal; 60-a second seal; 70-a third seal; 80-a support;

11-a mounting plate; 12-a fastener; 13-a handle; 14-a first accommodating groove; 21-a first cover plate; 22-a second cover plate; 31-a containment body; 32-a positioning body; 33-a first closure; 34-a liquid inlet pipe; 35-an exhaust pipe; 36-a liquid discharge port; 41-snap mounting plate; 42-hook of the hasp; 43-hasp clips; 44-snap ring; 81-a second receiving groove;

211 — a first via; 212-first seal groove; 213-a second seal groove; 214-a vent; 215-a second closure; 221-a second via; 222-a yield slot; 223-third via; 321-a connecting part; 322-an abutment; 323-opening.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the description of the present invention, it should be noted that the terms "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings or orientations or positional relationships that are conventionally placed when the products of the present invention are used, and are used only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the devices or elements to be referred to must have specific orientations, be constructed in specific orientations, and operate, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance. In the description of the present invention, "a plurality" means two or more unless otherwise specified.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed" and "connected" are to be interpreted broadly, e.g., as being either fixedly connected, detachably connected, or integrally connected; either mechanically or electrically. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

In the present invention, unless otherwise expressly stated or limited, "above" or "below" a first feature means that the first and second features are in direct contact, or that the first and second features are not in direct contact but are in contact with each other via another feature therebetween. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.

As shown in fig. 1 to 4, the present embodiment provides a biological reagent container for gene sequencing, comprising:

the box body 10 is of a hollow structure; one end of the box body 10 along the first direction is provided with an opening; in this embodiment, the first direction is the height direction of the box body 10;

the reagent accommodating part 30 comprises an accommodating body 31 and a positioning body 32 arranged at one end of the accommodating body 31, wherein the accommodating body 31 is arranged in the box body 10;

a cover plate assembly 20 covering the opening; the positioning device comprises a first cover plate 21 and a second cover plate 22 which are sequentially arranged along the direction far away from the box body 10, wherein the second cover plate 22 is used for pressing the positioning body 32 on the first cover plate 21; a first sealing element 50 is arranged between the first cover plate 21 and the box body 10; a second seal 60 is arranged between the positioning body 32 and the first cover plate 21.

In the embodiment, the cover plate assembly 20 presses the positioning body 32 to assemble the reagent accommodating part 30 and the box body 10, so that the reagent accommodating part 30 is stably placed, mutual collision caused by shaking is avoided, and stable transfer and loading of biological reagent containers are facilitated; simultaneously through the setting of first sealing member 50 and second sealing member 60, combine the pressure of apron to establish the effect, realized that reagent holds between 30 and the box body 10 well sealed, not only can avoid reagent to leak and cause the pollution to biological reagent container, can also avoid the doping between the reagent to the influence of testing result accuracy.

The first cover 21 is fixedly connected to the box 10 to ensure the stability of the positioning body 32 supported on the first cover 21. Referring to fig. 3 and 4, further, the first cover 21 is fastened to the box 10 by a fastener 12; specifically alternatively, the fastener 12 may be a screw or bolt; the fastening member 12 is fastened to the case 10 after passing through the first cover 21 and the first sealing member 50 in turn. Further, a third sealing member 70 is disposed between the fastening member 12 and the first cover plate 21, so as to further prevent leakage from a gap between the fastening member 12 and the first cover plate 21. Further alternatively, an annular mounting plate 11 is disposed on the end surface of the open end of the box body 10, and the first cover plate 21 is fixedly mounted on the annular mounting plate 11. Optionally, the first sealing element 50 is a ring-shaped sealing ring, and its shape follows the shape of the mounting plate 11; the mounting plate 11 not only increases the assembly space of the first fastening member 12 and ensures the connection strength between the first cover plate 21 and the box body 10, but also increases the area of the corresponding first sealing element 50 and improves the sealing effect of the first sealing element 50. Further, referring to fig. 4, a first sealing groove 212 is disposed at the bottom of the first cover plate 21, and the first sealing element 50 is accommodated in the first sealing groove 212, thereby achieving the positioning and stable placement of the first sealing element 50.

Specifically, referring to fig. 6, the positioning body 32 is a T-shaped structure, and includes a connecting portion 321 and an abutting portion 322 that are connected perpendicularly to each other, the connecting portion 321 is connected to the accommodating body 31, and two end faces of the abutting portion 322 along the first direction are respectively abutted to the first cover plate 21 and the second cover plate 22, so that the reagent accommodating member 30 can be stably supported on the first cover plate 21, thereby ensuring stable placement of the reagent accommodating member in the biological reagent container.

Further, referring to fig. 2 and 3, the first cover plate 21 is provided with a first through hole 211, and the size of the first through hole 211 is larger than the size of the connecting portion 321 between the accommodating body 31 and the positioning body 32, but smaller than the size of the abutting portion 322 of the positioning body 32, so that when the reagent accommodating member 30 is put into the cartridge 10 through the first through hole 211, the accommodating body 31 and a part of the positioning body 32 of the reagent accommodating member 30 enter the cartridge 10, and the abutting portion 322 of the positioning body 32 is supported on the first cover plate 21. Further alternatively, referring to fig. 3 and 4, a second sealing groove 213 is disposed at the periphery of the first through hole 211, and the second sealing element 60 is disposed in the second sealing groove 213, so that the positioning and stable placement of the second sealing element 60 are ensured, and the sealing effect is better achieved. Alternatively, the second seal 60 may be provided between the annular sidewall of the second seal groove 213 and the annular sidewall of the abutment portion 322 of the positioning body 32; the second seal 60 may be provided between the bottom surface of the contact portion 322 and the top surface of the second seal groove 213, that is, the contact portion 322 and the second seal groove may be provided in this order in the first direction; in this embodiment, the latter is preferred, and thus the second sealing element 60 can be sufficiently compressed between the positioning body 32 and the first cover plate 21 when the second cover plate 22 is pressed, so as to achieve a better sealing effect. In the embodiment, the positioning body 32 has a cylindrical structure, and the second sealing element 60 is an annular sealing ring, so as to achieve better sealing effect.

Further alternatively, referring to fig. 4, an abdicating groove 222 is formed at the bottom of the second cover plate 22, and the abutting portion 322 of the positioning body 32 can be accommodated in the abdicating groove 222 and abut against the groove top of the abdicating groove 222, so as to reduce the size of the whole biological reagent container in the first direction. Since the second cover plate 22 indirectly presses the first cover plate 21 even in the process of pressing the setting body 32, the first seal 50 is compressed, and the sealing effect is improved. In specific implementation, the non-grooved part on the bottom surface of the second cover plate 22 can be abutted to the first cover plate 21, so that the contact area between the second cover plate 22 and the first cover plate 21 is increased, the pressure on the first cover plate 21 when the second cover plate 22 is pressed is increased, and the sealing effect of the first sealing element 50 is further improved.

Since the sealing member is generally an elastic member, a large pressing force is usually required to press the sealing member in order to ensure the sealing effect of the sealing member. Thus, referring to fig. 1, the second cover 22 is connected to the case 10 by a snap assembly 40; alternatively, the box body 10 is a cubic structure, and the snap components 40 are provided in plurality, and at least one snap component 40 is provided on each side of the cubic structure, so as to realize uniform pressing of the second cover plate 22. Further, referring to fig. 2 and 7, the buckle assembly 40 includes: a hasp mounting plate 41 arranged on the box body 10; a hook latch 42 provided on the second cover plate 22; the hasp buckle plate 43 is rotatably arranged on the hasp mounting plate 41; a buckle ring 44 rotatably provided on the buckle plate 43; in specific implementation, the buckle plate 43 is opened to place the buckle ring 44 in the buckle hook 42, and then the buckle plate 43 is closed to drive the buckle ring 44 to hook the buckle hook 42 downwards, so as to give a certain pressing force to the second cover plate 22, and the second cover plate 22 further compresses the sealing element.

The reagent container 30 needs to be connected to the outside so that external liquid can be timely introduced into the container body 31 or the reagent in the container body 31 can be discharged, which is referred to as reagent loading. Therefore, referring to fig. 6, the positioning body 32 is opened with an opening 323 to communicate the outside and the accommodating body 31. Further, referring to fig. 2 and 4, the reagent holding member 30 further includes a first blocking member 33 for blocking the opening 323 so that the reagent is not scattered when the reagent is not required to be used. Optionally, the first closure 33 is a first plug. Furthermore, the second cover plate 22 is provided with a second through hole 221, and the first blocking piece 33 of the reagent accommodating part 30 is exposed from the second through hole 221, so that when the biological reagent container is loaded, the loading of the reagent can be completed only by pulling out the first blocking piece 33 without disassembling the cover plate assembly 20.

Alternatively, the reagent holding member 30 employs a reagent bag; preferably, the reagent bag is a soft bag; after the first plugging piece 33 is pulled out, the reagent can be supplied by air pressure pressurization; the reagent container 30 is also reduced in weight by a bag-pack form, and the phenomenon of collision and breakage easily occurring when a glass bottle body is used is avoided. Specifically, in the case of using a soft bag for the reagent accommodating part 30, in order to implement pressurized liquid supply, referring to fig. 2 and 5, a vent hole 214 is further formed on the first cover plate 21, the vent hole 214 is communicated with the case body 10, so that gas can enter the case body 10 through the vent hole 214, and since the case body 10 is sufficiently sealed by the cover plate assembly 20, when the air pressure in the case body 10 is increased, the soft bag is squeezed to complete liquid supply. Further, a second blocking member 215 is further disposed at the vent hole 214 for blocking the vent hole 214, so that external impurities do not enter the inside of the case body 10 when the air intake device is not connected. Further, when the reagent accommodating part 30 adopts a reagent bag, the accommodating body 31 and the positioning body 32 are respectively a bag body of the reagent bag and a skeleton head of the bag body head, and the skeleton head can be supported on the first cover plate 21, so that the reagent bag is stably placed. Optionally, the skeleton head and the bag body are formed by a thermoforming process.

The reagent holding member 30 is provided in plural, and thus, it is possible to store plural kinds of reagents. In one embodiment, the number of reagent holding members 30 may be set to at least two. In this embodiment, eight reagent containers 30 are provided. Optionally, the reagent holding member 30 is used for holding a sample to be detected; further, the reagent holding member 30 may also be used to hold waste liquid generated during the detection process. When the reagent container 30 is used as a waste liquid storage, the reagent container 30 can be selected to be a waste liquid bottle with a bottle-shaped structure, and the container body 31 and the positioning body 32 are respectively a bottle body and a bottle cap of the waste liquid bottle. Further, referring to fig. 8, when the reagent container 30 is used as a waste liquid bottle, a liquid inlet pipe 34 and an exhaust pipe 35 are disposed on a bottle cap of the waste liquid bottle to communicate the outside with the inside of the bottle, the liquid inlet pipe 34 is used for introducing waste liquid, and the exhaust pipe 35 is used for exhausting gas in the waste liquid bottle to ensure smooth entry of the waste liquid. Further, the inlet pipe 34 and the outlet pipe 35 are closed by a second plug. Further, the bottom of bottle still sets up drain 36 for the waste liquid of discharging, drain 36 adopts the shutoff of third plug.

Referring to fig. 3, 4 and 9, the other end of the case 10 in the first direction is closed, and a handle 13 is provided on an end surface of the closed end; after the reagent is loaded, the whole biological reagent container can be taken by a detection person through the handle 13, so that the biological reagent container is convenient to transfer. Optionally, a first accommodating groove 14 is further formed in the end face of the closed end of the box body 10; the handle 13 is a reversible handle 13 and has two states of retraction and extension; when the reagent is loaded, the handle 13 is in a retraction state and is hidden in the first accommodating groove 14, so that the biological reagent container can be seated on the supporting surface and kept stably; when the biological reagent container is transferred, the handle 13 is in the unfolded state, which is convenient for the operator to hold.

The handle 13 and the reagent loading end are located at both ends of the first direction of the case body 10, so that the reagent accommodating member 30 is inverted when the container for biological reagent is carried; because the box body 10 is hollow, the length of the accommodating body 31 is also longer than that of the positioning body 32, the accommodating body 31 is easy to shake in the process of lifting and taking the biological reagent container, and particularly when reagent is loaded in a reagent bag, the bag body is easy to collapse or deform, so that subsequent reagent loading is influenced. Therefore, referring to fig. 3 and 4, the biological reagent container further includes a support body 80, the support body 80 being accommodated in the cartridge body 10 and having an outer contour following an inner wall contour of the cartridge body 10; the second holding tank 81 has been seted up on the supporter 80, and reagent holds 30 and locates in the second holding tank 81, and then the cell wall of second holding tank 81 can fix a position and effectively support holding body 31, avoids taking place to warp at biological reagent container transfer in-process, guarantees that reagent holds the steadiness that 30 placed in box body 10. Alternatively, the second receiving groove 81 is a through groove disposed throughout the support body 80 in the first direction. Optionally, supporter 80 adopts the cotton material of bubble to make, and the cotton material quality of bubble is light, and has certain elasticity, can reduce biological reagent container weight while, can also implement effective support to the test tube holding piece.

In specific implementation, the first cover 21 is tightly connected with the box body 10, and the buckle assembly 40 is in an open state; then, after the reagent accommodating part 30 is loaded with the reagent, the reagent enters the box body 10 through the first through hole 211 of the first cover plate 21; a second seal 60 is placed in the second seal groove 213, and the positioning body 32 is finally supported on the second seal 60 in the second seal groove 213; covering the second cover plate 22 and closing the buckle assembly 40, wherein the second cover plate 22 compresses the second sealing member 60 to complete the assembly of the reagent accommodating member 30 and the biological reagent container; the handle 13 is lifted to realize the transfer and loading of the biological reagent container.

It is to be noted that the foregoing is only illustrative of the preferred embodiments of the present invention and the technical principles employed. It will be understood by those skilled in the art that the present invention is not limited to the particular embodiments described herein, but is capable of various obvious changes, rearrangements and substitutions as will now become apparent to those skilled in the art without departing from the scope of the invention. Therefore, although the present invention has been described in greater detail by the above embodiments, the present invention is not limited to the above embodiments, and may include other equivalent embodiments without departing from the spirit of the present invention, and the scope of the present invention is determined by the scope of the appended claims.

Claims

1. A biological reagent container for use in gene sequencing, comprising:

the box body (10), one end of the box body (10) along a first direction is provided with an opening;

the reagent accommodating part (30) comprises an accommodating body (31) and a positioning body (32) arranged at one end of the accommodating body (31), and the accommodating body (31) is arranged in the box body (10);

the cover plate component (20) is covered at the opening; the cover plate assembly (20) comprises a first cover plate (21) and a second cover plate (22) which are sequentially arranged along the direction far away from the box body (10), and the second cover plate (22) is used for pressing the positioning body (32) on the first cover plate (21);

a first sealing element (50) is arranged between the first cover plate (21) and the box body (10); a second seal (60) is arranged between the positioning body (32) and the first cover plate (21).

2. The biological reagent container according to claim 1, wherein the first cover plate (21) is connected with the box body (10) through a fastener (12), and the fastener (12) is locked on the box body (10) after sequentially passing through the first cover plate (21) and the first sealing member (50); a third seal (70) is provided between the fastener (12) and the first cover plate (21).

3. The biological reagent container according to claim 1, wherein the positioning body (32) is of a T-shaped structure and comprises a connecting portion (321) and an abutting portion (322), the connecting portion (321) is connected to the accommodating body (31), and two end faces of the abutting portion (322) along the first direction abut against the first cover plate (21) and the second cover plate (22), respectively.

4. The container according to claim 3, wherein the second cover plate (22) has a recess (222) formed in a bottom thereof, and the abutting portion (322) is received in the recess (222) and abuts against a top of the recess (222).

5. The biological reagent container according to claim 1, wherein the first cover plate (21) is provided with a first through hole (211), and the accommodating body (31) of the reagent accommodating member (30) enters the box body (10) from the first through hole (211); the periphery of the first through hole (211) is provided with a sealing groove, and the second sealing element (60) is arranged in the sealing groove and is simultaneously abutted against the groove bottom of the sealing groove and the bottom surface of the positioning body (32).

6. The bioreagent container according to claim 1, wherein the second cover (22) is connected to the cassette (10) by a snap assembly (40) such that the second cover (22) compresses the seal.

7. The biological reagent container according to claim 6, wherein the biological reagent container is a cube structure, at least one snap assembly (40) being provided on each side of the cube structure.

8. The biological reagent container according to claim 1, wherein the positioning body (32) is provided with an opening (323) for communicating the containing body (31) with the outside; the reagent holding member (30) further comprises a first blocking member (33) for blocking the opening (323);

a second through hole (221) is formed in the second cover plate (22), and the first blocking piece (33) is exposed from the second through hole (221).

9. The biological reagent container according to claim 1, wherein the other end of the cartridge body (10) in the first direction is closed and provided with a handle (13); still set up first holding tank (14) on the terminal surface of the blind end of box body (10), handle (13) can be withdrawed to in first holding tank (14).

10. The biological reagent container according to claim 1, further comprising a support body (80), wherein the support body (80) is disposed in the cartridge body (10), the support body (80) has a second receiving groove (81) formed therein, and the receiving body (31) is disposed in the second receiving groove (81).

11. The biological reagent container according to claim 1, wherein the number of the reagent holding members (30) is at least two.

12. A biological reagent container according to claim 1, wherein the reagent holding member (30) is a flexible bag; the first cover plate (21) is provided with a vent hole (214), and the vent hole (214) is communicated with the box body (10); a second blocking piece (215) is arranged at the vent hole (214).