CN111558080A

CN111558080A - Antibacterial silk suture line with double-light response function and preparation method thereof

Info

Publication number: CN111558080A
Application number: CN202010542618.6A
Authority: CN
Inventors: 杨学涛; 王小磊; 曹飞; 曾斌
Original assignee: Nanchang University
Current assignee: Nanchang University
Priority date: 2020-06-15
Filing date: 2020-06-15
Publication date: 2020-08-21

Abstract

The invention discloses an antibacterial silk suture with double-light response function and a preparation method thereof, wherein zinc nitrate hexahydrate, ammonia water and hexamethylenetetramine are used as main raw materials, and are subjected to one-step reaction and then are subjected to reaction with sodium citrate, hydroxypropyl methylcellulose, carbon powder and lignin, and the obtained product is subjected to freeze drying and microwave treatment to obtain porous zinc oxide; the porous zinc oxide is loaded on the silk thread to obtain the antibacterial silk thread suture thread with the double-light response function, the antibacterial performance of the porous zinc oxide can be further enhanced under the excitation of yellow light, and the temperature of the zinc oxide is increased under the excitation of near infrared light so as to accelerate the degradation of the suture thread. The method has the advantages of simple and safe process, no need of adding organic solvent, simple operation method, low requirement on equipment and suitability for large-scale production and industrialization.

Description

Antibacterial silk suture line with double-light response function and preparation method thereof

Technical Field

The invention relates to the field of silk suture lines, in particular to an antibacterial silk suture line with a double-light response function and a preparation method thereof.

Background

Surgical Site Infection (SSI) remains a ubiquitous problem in modern surgery. Based on the data from the united states center for Disease Control and prevention (CDC), the overall incidence of SSI in the united states is estimated to be 2.8%, which corresponds to 756000 patients per year. Due to inconsistency among studies, the incidence of SSI reported by european countries is 1.5% -20%; however, the actual SSI incidence should be underestimated, which indicates that SSI is also a significant problem in europe. SSI is the third leading cause of nosocomial infections in China, second only to urinary system infections and pneumonia. The data show that SSI accounts for 14-16% of hospital infections throughout hospitalization, with 38% of hospital infections being associated with SSI, with 2/3 being an incisional infection among SSI. Incisions are inevitable away from the use of silk sutures, however, silk sutures currently used clinically do not reduce the incidence of SSI, which is overly dependent on antibiotics. Therefore, there is a need for a suture thread that can prevent bacterial infection from the source. In addition, the degradation of the silk suture is a troublesome problem in clinical use, the degradation of the wound can occur after the wound is not completely healed, and the prognosis of the wound is not optimistic. Therefore, an ideal suture of wire should be able to controllably degrade after wound healing. In conclusion, a silk thread suture based on surface modification of porous zinc oxide is developed, and the porous zinc oxide not only endows the silk thread with excellent antibacterial effect, but also can accelerate the degradation of the silk thread under the irradiation of near infrared light.

Disclosure of Invention

The invention aims to solve the problems, provides an antibacterial silk suture with a double-light response function and a preparation method thereof, and aims to solve the technical problems that the existing suture is weak in antibacterial effect and cannot realize controllable degradation, and the like, and the technical scheme adopted by the invention is as follows:

an antibacterial silk suture with double-light response function and a preparation method thereof are disclosed, wherein the preparation process comprises the following steps:

1) dissolving 0.56g of NH3. H2O, 2.975g of Zn (NO3) 2.6H 2O and 0.7g C6 g of Zn 12N4 in 20ml of deionized water in sequence, and sealing and stirring for 10min to obtain a solution A;

2) reacting the obtained solution A at a constant temperature of 65 ℃ for 15min to obtain a solution B;

3) taking 100ml of the solution B, sequentially adding 0.14g of sodium citrate, 0.1g of hydroxypropyl methyl cellulose, 0.1g of carbon powder and 0.1g of lignin, and reacting at a constant temperature of 85 ℃ for 12 hours;

4) washing the product obtained in the step 3) with purified water for 2 times, then washing with absolute ethyl alcohol for 2 times, and then washing with purified water for 2 times;

5) and (3) carrying out vacuum freeze drying on the product obtained in the step (4), putting the product into a microwave oven for pyrolysis for 15min to obtain porous zinc oxide, and uniformly loading the porous zinc oxide on the surface of the silk thread to obtain the antibacterial silk thread with the double-light response function.

The adding sequence of NH3. H2O, Zn (NO3) 2.6H 2O and hexamethylene tetramine in the step 1).

The isothermal reactions in step 2) and step 3) are all carried out under the condition of water bath.

In the stirring process of the step 1) and the constant-temperature reaction process of the step 2), the container is sealed by a cover.

The antibacterial silk suture line with the double-light response function has the advantages that double light is yellow light and near infrared light, porous zinc oxide is uniformly loaded on the surface of the suture line, the antibacterial performance of the porous zinc oxide can be enhanced under the excitation of the yellow light, the porous zinc oxide can be heated under the excitation of the near infrared light, and therefore the degradation of the silk suture line is accelerated.

The invention has the beneficial effects that:

1. the porous adsorbent has good adsorption effect;

2. the yellow light can be responded, the antibacterial effect can be achieved without ultraviolet excitation, and the yellow light is mild and has small stimulation to eyes, so that the use is safer;

3. the photothermal effect is applied to the field of silk suture lines for the first time, and the degradation controllability can be realized;

4. the method has the advantages of simple and safe process, no need of adding organic solvent, simple operation method, low requirement on equipment and suitability for large-scale production and industrialization.

Drawings

FIG. 1 is a SEM comparison of the surface of a silk suture prepared by the method of the present invention and a conventional silk suture;

FIG. 2 is a schematic view showing the antibacterial performance of a suture thread made by the method of the present invention;

FIG. 3 is a schematic representation of the response of a silk suture made by the method of the present invention to near infrared light;

FIG. 4 is a schematic diagram of wound healing in a mouse according to an embodiment of the present invention;

FIG. 5 is a graph showing the comparison of bacterial infection concentration on wounds and the healing rate of wounds in mice according to an embodiment of the present invention;

FIG. 6 is a schematic representation of HE staining of muscle and skin of a wound in a mouse according to an embodiment of the present invention.

Detailed Description

The invention is further described below with reference to the figures and examples.

Example (b): see fig. 1-6.

Simultaneously using the antibacterial silk suture and the common silk suture for suturing the back wound of the rat, and irradiating yellow light during the process, and observing the curative effect; the results are shown in FIGS. 4 to 6.

Fig. 4 is a graph of the healing effect of the mouse wound of the common silk suture line control group a, the porous zinc oxide-loaded silk suture line control group B and the porous zinc oxide-loaded silk suture line yellow light irradiation experiment group C in sequence from left to right, and it can be observed that the healing effect of the yellow light irradiation experiment group C is obviously better than that of the control groups a and B.

Under the irradiation of yellow light, the wound of the mouse sutured with the suture loaded with the porous zinc oxide in the graph of fig. 5 has lower bacteria concentration in the wound and faster healing speed of the wound surface; the HE staining results of muscle and skin in fig. 6 also show that under yellow light irradiation, the wound of mice sutured with porous zinc oxide-loaded sutures had less inflammatory exudate and inflammatory cell infiltration, less proliferation of local parenchymal cells and interstitial cells, and mild inflammatory symptoms.

Although the present invention has been described in detail with reference to the above embodiments, it should be understood by those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof without departing from the spirit and scope of the invention.

Claims

1. An antibacterial silk suture with double-light response function and a preparation method thereof are characterized in that the preparation process comprises the following steps:

2. The suture of antibacterial silk thread having a double photoresponse function and the process for preparing the same according to claim 1, wherein the order of putting NH3. H2O, Zn (NO3) 2.6H 2O and hexamethylenetetramine in step 1) is selected.

3. The suture of antibacterial silk thread with double photoresponse function and its preparation method according to claim 1, wherein the isothermal reactions in step 2) and step 3) are carried out under water bath conditions.

4. The suture thread of antibacterial silk having a dual photoresponse function and the process for preparing the same according to claim 1, wherein the vessel is sealed at the mouth of the vessel by a cap during the stirring process of step 1) and the isothermal reaction process of step 2).

5. The antibacterial silk suture thread with double photoresponse function and the preparation method thereof according to claim 1, wherein the double light is yellow light and near infrared light, the surface of the antibacterial silk suture thread with double photoresponse function is uniformly loaded with porous zinc oxide, the antibacterial property of the porous zinc oxide can be enhanced by the porous zinc oxide under the excitation of the yellow light, and the temperature of the porous zinc oxide can be raised under the excitation of the near infrared light, so that the degradation of the silk suture thread is accelerated.