CN111551718A - 一种检测前列腺癌患者外周血循环肿瘤细胞pd-l1表达的免疫荧光试剂盒及检测方法 - Google Patents

一种检测前列腺癌患者外周血循环肿瘤细胞pd-l1表达的免疫荧光试剂盒及检测方法 Download PDF

Info

Publication number
CN111551718A
CN111551718A CN202010312650.5A CN202010312650A CN111551718A CN 111551718 A CN111551718 A CN 111551718A CN 202010312650 A CN202010312650 A CN 202010312650A CN 111551718 A CN111551718 A CN 111551718A
Authority
CN
China
Prior art keywords
peripheral blood
fluorescence
prostate cancer
labeled goat
filter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
CN202010312650.5A
Other languages
English (en)
Chinese (zh)
Inventor
李胜
王焕昇
李�浩
高德海
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shandong First Medical University and Shandong Academy of Medical Sciences
Original Assignee
Shandong First Medical University and Shandong Academy of Medical Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shandong First Medical University and Shandong Academy of Medical Sciences filed Critical Shandong First Medical University and Shandong Academy of Medical Sciences
Priority to CN202010312650.5A priority Critical patent/CN111551718A/zh
Publication of CN111551718A publication Critical patent/CN111551718A/zh
Priority to PCT/CN2021/087985 priority patent/WO2021213292A1/fr
Withdrawn legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C12N5/0694—Cells of blood, e.g. leukemia cells, myeloma cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57407—Specifically defined cancers
    • G01N33/57434—Specifically defined cancers of prostate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C12N2509/10—Mechanical dissociation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70503—Immunoglobulin superfamily, e.g. VCAMs, PECAM, LFA-3
    • G01N2333/70532—B7 molecules, e.g. CD80, CD86

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Oncology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Hospice & Palliative Care (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202010312650.5A 2020-04-20 2020-04-20 一种检测前列腺癌患者外周血循环肿瘤细胞pd-l1表达的免疫荧光试剂盒及检测方法 Withdrawn CN111551718A (zh)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN202010312650.5A CN111551718A (zh) 2020-04-20 2020-04-20 一种检测前列腺癌患者外周血循环肿瘤细胞pd-l1表达的免疫荧光试剂盒及检测方法
PCT/CN2021/087985 WO2021213292A1 (fr) 2020-04-20 2021-04-19 Kit de test d'immunofluorescence permettant de mesurer l'expression de pd-l1 dans des cellules tumorales circulantes dans le sang périphérique chez un patient atteint d'un cancer de la prostate, et méthode de mesure

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010312650.5A CN111551718A (zh) 2020-04-20 2020-04-20 一种检测前列腺癌患者外周血循环肿瘤细胞pd-l1表达的免疫荧光试剂盒及检测方法

Publications (1)

Publication Number Publication Date
CN111551718A true CN111551718A (zh) 2020-08-18

Family

ID=72003856

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010312650.5A Withdrawn CN111551718A (zh) 2020-04-20 2020-04-20 一种检测前列腺癌患者外周血循环肿瘤细胞pd-l1表达的免疫荧光试剂盒及检测方法

Country Status (2)

Country Link
CN (1) CN111551718A (fr)
WO (1) WO2021213292A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021213322A1 (fr) * 2020-04-21 2021-10-28 山东第一医科大学(山东省医学科学院) Kit d'immunofluorescence pour détecter l'expression pd-l1 de cellules tumorales circulantes du sang périphérique d'un patient atteint d'un cancer du rein et procédé de détection
WO2021213292A1 (fr) * 2020-04-20 2021-10-28 山东第一医科大学(山东省医学科学院) Kit de test d'immunofluorescence permettant de mesurer l'expression de pd-l1 dans des cellules tumorales circulantes dans le sang périphérique chez un patient atteint d'un cancer de la prostate, et méthode de mesure

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105588943B (zh) * 2016-01-28 2018-03-02 山东省肿瘤防治研究院 一种胃癌患者外周血循环肿瘤细胞Her‑2基因的检测方法
CN106198984B (zh) * 2016-08-22 2019-07-05 上海立闻生物科技有限公司 非小细胞肺癌患者外周血循环肿瘤细胞pdl1基因的检测方法
US20180267046A1 (en) * 2017-03-14 2018-09-20 Micareo Taiwan Co., Ltd. Methods for diagnosing and treating cancer
WO2019089740A1 (fr) * 2017-11-03 2019-05-09 Dana-Farber Cancer Institute, Inc. Biomarqueurs de réponse clinique et de bienfait d'une thérapie par inhibiteur de point de contrôle immunitaire
CN110632292A (zh) * 2019-06-12 2019-12-31 江苏莱尔生物医药科技有限公司 一种检测pd-l1和cd8抗原的免疫荧光试剂盒及应用方法
CN110716041B (zh) * 2019-10-23 2023-04-18 郑州大学 一种用于胃癌早期筛查和诊断的血清蛋白标志物、试剂盒及检测方法
CN111551718A (zh) * 2020-04-20 2020-08-18 山东第一医科大学(山东省医学科学院) 一种检测前列腺癌患者外周血循环肿瘤细胞pd-l1表达的免疫荧光试剂盒及检测方法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021213292A1 (fr) * 2020-04-20 2021-10-28 山东第一医科大学(山东省医学科学院) Kit de test d'immunofluorescence permettant de mesurer l'expression de pd-l1 dans des cellules tumorales circulantes dans le sang périphérique chez un patient atteint d'un cancer de la prostate, et méthode de mesure
WO2021213322A1 (fr) * 2020-04-21 2021-10-28 山东第一医科大学(山东省医学科学院) Kit d'immunofluorescence pour détecter l'expression pd-l1 de cellules tumorales circulantes du sang périphérique d'un patient atteint d'un cancer du rein et procédé de détection

Also Published As

Publication number Publication date
WO2021213292A1 (fr) 2021-10-28

Similar Documents

Publication Publication Date Title
CN111562375B (zh) 一种检测胃癌患者外周血循环肿瘤细胞pd-l1表达的免疫荧光试剂盒及检测方法
CN111521796A (zh) 一种检测肾癌患者外周血循环肿瘤细胞pd-l1表达的免疫荧光试剂盒及检测方法
CN111638359A (zh) 检测小细胞肺癌患者外周血循环肿瘤细胞pd-l1基因突变的免疫荧光试剂盒及检测方法
CN111521794A (zh) 检测小细胞肺癌患者外周血循环肿瘤细胞nse基因突变的免疫荧光试剂盒及检测方法
CN111638357A (zh) 非小细胞肺癌患者外周血循环肿瘤细胞E-Cadherin突变的免疫荧光试剂盒及方法
CN111638341A (zh) 一种检测小细胞肺癌患者外周血循环肿瘤细胞pd-l1基因突变的试剂盒及检测方法
CN111521790A (zh) 一种通过外周血循环肿瘤细胞检测结直肠癌患者pd-l1基因突变的非诊断目的方法
CN111521798A (zh) 一种检测肾癌患者外周血循环肿瘤细胞pd-l1基因突变的试剂盒及检测方法
CN111551718A (zh) 一种检测前列腺癌患者外周血循环肿瘤细胞pd-l1表达的免疫荧光试剂盒及检测方法
WO2021213310A1 (fr) Kit d'immunofluorescence pour la détection de l'expression du gѐne pd-l1 chez un patient atteint d'un carcinome épidermoïde œsophagien au moyen de cellules tumorales circulantes du sang périphérique
CN111521793A (zh) 检测非小细胞肺癌患者外周血循环肿瘤细胞cea基因突变的免疫荧光试剂盒及检测方法
CN111521801A (zh) 通过外周血循环肿瘤细胞检测结直肠癌患者braf基因v600e突变表达的试剂盒
CN111679077A (zh) 肾细胞癌患者外周血循环肿瘤细胞E-Cadherin表达的免疫荧光试剂盒及检测方法
CN111521795A (zh) 一种检测非小细胞肺癌患者外周血循环肿瘤细胞pd-l1基因突变的试剂盒及检测方法
CN111638358A (zh) 小细胞肺癌患者外周血循环肿瘤细胞E-Cadherin突变的免疫荧光试剂盒及方法
CN111521791A (zh) 一种检测小细胞肺癌患者外周血循环肿瘤细胞nse基因突变的试剂盒及检测方法
CN111521800A (zh) 通过外周血循环肿瘤细胞检测结直肠癌患者pd-l1基因表达的免疫荧光试剂盒
CN111638349A (zh) 一种检测胃癌患者外周血循环肿瘤细胞ca125表达的免疫荧光试剂盒及检测方法
CN111521792A (zh) 检测非小细胞肺癌患者外周血循环肿瘤细胞pd-l1基因突变的免疫荧光试剂盒及方法
CN111596056A (zh) 一种检测前列腺癌患者外周血循环肿瘤细胞pd-l1基因突变的试剂盒及检测方法
CN111551728A (zh) 一种检测胰腺癌患者外周血循环肿瘤细胞pd-l1表达的免疫荧光试剂盒及检测方法
CN111562376A (zh) 一种检测胃癌患者外周血循环肿瘤细胞pd-l1基因突变的试剂盒及检测方法
CN111551727A (zh) 检测非小细胞肺癌患者外周血循环肿瘤细胞alk基因突变的免疫荧光试剂盒及检测方法
CN111521789A (zh) 一种检测胰腺癌患者外周血循环肿瘤细胞ca199表达的免疫荧光试剂盒及检测方法
CN111638345A (zh) 检测非小细胞肺癌患者外周血循环肿瘤细胞E-Cadherin基因突变的试剂盒及方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WW01 Invention patent application withdrawn after publication
WW01 Invention patent application withdrawn after publication

Application publication date: 20200818