CN111551617A - Preparation method of faviravir molecularly imprinted paste electrode sensor - Google Patents
Preparation method of faviravir molecularly imprinted paste electrode sensor Download PDFInfo
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Abstract
The invention discloses a preparation method of a Faviravir molecular imprinting paste electrode sensor, which adopts nano gallium phosphide, graphene oxide, carbon quantum dots and 1-ethyl methyl ether-3-methylimidazole phosphate as raw materials to prepare a gallium phosphide/quantum dot paste electrode; modifying the gallium phosphide/quantum dot paste electrode by adopting gamma-glycidoxypropyltrimethoxysilane to obtain a gamma-glycidoxypropyltrimethoxysilane modified gallium phosphide/quantum dot paste electrode; preparing a Faviravir molecularly imprinted polymer by using Faviravir template molecules, tetraethylenepentamine and 2-aminopyrrole as monomers and trimethylolpropane triglycidyl ether as a cross-linking agent; modifying the Viravir molecular imprinting polymer on a gamma-glycidyl ether oxypropyl trimethoxy silane modified gallium phosphide/quantum dot paste electrode by adopting a dropping coating method to prepare the Faravravir molecular imprinting paste electrode sensor. The sensor has the advantages of high sensitivity, good specificity, rapid detection and repeated use.
Description
Technical Field
The invention relates to the technical field of a preparation method and rapid detection application of a molecular imprinting sensor, in particular to a preparation method of a faviravir molecular imprinting paste electrode sensor, which is used for detecting faviravir in a sample.
Background
Faviravir (also known as Favipiravir), a novel RNA-dependent RNA polymerase (RdRp) inhibitor, belongs to a broad-spectrum anti-influenza virus drug, and is used for treating novel and recurrent influenza. Research shows that the medicine has good antiviral effect on various RNA viruses except influenza virus, such as Ebola virus, arenavirus, bunyavirus, rabies virus and the like. According to the information published in 2020, 2, 15 and 15 days of the Ministry of science and technology, Favipiravir is one of three clinical trial western medicines for treating new coronary pneumonia, and initially shows a relatively obvious curative effect and relatively low adverse reaction for treating the new coronary pneumonia. Favilavir is expected to become a new choice for COVID-19 antiviral treatment of pneumonia. The method for measuring the Vilarvir by simple, quick, accurate and high-sensitivity research is very important for the performance of the medicine. At present, the method for detecting the Vilarvir mainly comprises high performance liquid chromatography, the accuracy of the methods is limited to a certain extent, instruments are expensive, professional personnel are required to operate, the detection period is long, and the application of the methods is also limited. In addition, because the content of the Vilarvir in a biological sample is low, and coexisting substances are mutually interfered, the accurate detection of the Vilarvir is difficult. Therefore, the method for detecting the Vilarvir, which has the advantages of good selectivity, high sensitivity and simple and convenient operation, has an important use value.
The molecular imprinting technique is one of the main methods for developing a highly selective material having a molecular recognition function, and is a technique that exhibits a high selective recognition property for a template molecule by forming a highly cross-linked rigid polymer around the template molecule, and removing the template molecule to leave a recognition site having a binding ability in a network structure of the molecularly imprinted polymer. This technology is receiving increasing attention due to its configuration predetermination and specific recognition. The molecular imprinting sensor prepared according to the technology plays an important role in drug analysis and life science research. The functional molecules are modified to the electrodes in an appropriate manner. The method is sensitive, rapid, simple, convenient, high in specificity, good in repeatability and economical, and is necessary for accurately and quantitatively measuring the content of the faviravir in the medicines and biological samples.
The crystal structure of gallium phosphide is zinc blende type, lattice constant is 5.447 + -0.06 angstrom, chemical bond is a mixed bond mainly comprising covalent bond, and its ionic bond componentAbout 20%, and the energy gap at 300K is 2.26eV, and the semiconductor is an indirect transition type semiconductor. Gallium phosphide, like other wide band gap group iii-v compound semiconductors (e.g., GaAS, InP), can bring the fermi level close to the middle of the band gap by introducing a deep center. Graphene, as a novel supercapacitor electrode material, exhibits numerous advantages as follows: ultra-high specific surface area, good conductivity, excellent stable chemical properties and wide potential window. The capacity of the single-layer graphene can reach 21 mu F/cm2However, in many cases, graphene is stacked in multiple layers, the area between layers is not effectively utilized, the actual capacity is lower than that of single-layer graphene, and the graphene is compounded with other nano structures to inhibit the overlapping of graphene sheets. The high-capacity transition metal nitride or the conductive polymer is laminated and compounded with the graphene, so that on one hand, the graphene is isolated by other substances, the agglomeration can be reduced, the mobility of the electrolyte is improved, and on the other hand, the graphene provides a three-dimensional network for electron conduction for the compounded pseudo-capacitance substance. The graphene-based composite material shows a synergistic effect, and maintains good rate performance while obtaining high capacity.
Carbon Quantum Dots (CQDs) are a recently discovered novel Carbon nano material with outstanding fluorescence, are composed of quasi-discrete spherical Carbon nano particles with the size less than 10 nm, and are an environment-friendly material with excellent water solubility, high biocompatibility and low toxicity. Research has shown many advantages: CQDs have up-conversion photoluminescence characteristics and can excite semiconductors to form more photo-generated electron-hole pairs; the CQDs have strong electron transmission performance, can effectively transfer and store photo-generated electrons, and achieve the effect of improving the charge separation efficiency. Therefore, CQDs are compounded with semiconductors and are important ways to improve the conductivity.
The invention aims to combine molecular imprinting with an electrochemical sensor and provides a preparation method of a Faviravir molecular imprinting paste electrode sensor.
Disclosure of Invention
Instruments and reagents
CHI660B electrochemical workstation (shanghai chenhua instruments), the experiment was carried out using a three-electrode system: the platinum wire electrode is an auxiliary electrode, the Ag/AgCl electrode is a reference electrode (SCE), and the Glassy Carbon Electrode (GCE) is a working electrode; KQ-250E model ultrasonic cleaner (Kunfeng ultrasonic Instrument Co., Ltd.).
Nano gallium phosphide, graphene oxide, tetraethylenepentamine, 2-aminopyrrole, trimethylolpropane triglycidyl ether, azodiisoheptanonitrile, faviravir, carbon quantum dots, 1-ethyl methyl ether-3-methylimidazole phosphate, gamma-glycidyl ether oxypropyltrimethoxysilane, glycerol, ethanol, N, N-dimethylformamide and a buffer solution; all the reagents are analytically pure, and the experimental water is secondary distilled water.
A preparation method of a Faviravir molecularly imprinted paste electrode sensor is characterized by comprising the following process steps:
(1) preparing a gallium phosphide/quantum dot paste electrode: adding the nano gallium phosphide into an agate mortar according to the following mass percentages: 44-48%, graphene oxide: 16-20%, 1-ethyl methyl ether-3-methylimidazole phosphate: 12-16%, carbon quantum dots: 4-6%, glycerin: 8-12%, ethanol: 6-10%, wherein the sum of the mass percentages of the components is one hundred percent, and the mixture is uniformly ground to obtain a mixture carbon paste; then the carbon paste is put into a glass tube which is connected with a lead and has an inner diameter of phi 5mm, and the glass tube is compacted, dried, polished by polishing powder, polished and washed by deionized water, thus obtaining the gallium phosphide/quantum dot paste electrode;
(2) preparing a gamma-glycidoxypropyltrimethoxysilane modified gallium phosphide/quantum dot paste electrode: adding ethanol into a reactor according to the mass percentage concentration of the following components: 70-74% of gamma-glycidyl ether oxypropyl trimethoxysilane: 26-30%, wherein the sum of the mass percentages of the components is one hundred percent, stirring and dissolving, putting the polished gallium nitride paste electrode into the electrode, soaking the electrode at room temperature for 4 hours, heating the electrode to 55 +/-2 ℃, reacting at constant temperature for 4 hours, taking out the electrode, washing the electrode with absolute ethyl alcohol, and drying to obtain the gamma-glycidyl ether oxypropyl trimethoxysilane modified gallium phosphide/quantum dot paste electrode;
(3) preparation of a Faviravir molecularly imprinted polymer: adding ethanol into a reactor according to the following composition mass percentage: 66-72%, tetraethylenepentamine: 8-12%, 2-aminopyrrole: 6-10%, favilavir: 4-8%, trimethylolpropane triglycidyl ether: 4-8%, azobisisoheptonitrile: 1.0-3.0%, the sum of the contents of all the components is one hundred percent, stirring and dissolving, introducing argon to remove oxygen for 15min, carrying out anaerobic atmosphere, stirring and reacting at 55 +/-2 ℃ for 2-4 h, carrying out solid-liquid separation, and adding alcohol into the obtained product: soaking the mixed solution with the volume ratio of acetic acid being 8:1 for 8 hours, washing for multiple times, removing template molecules, and drying to obtain a faviravir molecularly imprinted polymer;
(4) preparing a Faviravir molecularly imprinted paste electrode sensor: adding N, N-dimethylformamide into a reactor according to the mass percentage concentration of the following components: 95-97%, vinyl resin: 1.0-3.0%, Faviravir molecularly imprinted polymer: 1.0-3.0%, wherein the sum of the contents of all the components is one hundred percent, and heating and dissolving to prepare a faviravir molecularly imprinted polymer modification solution; and (3) dropwise adding 20-30 mu L of the solution into the gamma-glycidoxypropyltrimethoxysilane modified gallium phosphide/quantum dot paste electrode prepared in the step (2), placing the electrode under an infrared lamp, and volatilizing a dry solvent to obtain the Faviravir molecularly imprinted paste electrode sensor.
The carbon quantum dots in the step (1) are oil-soluble carbon quantum dots.
In step (3), the mole of tetraethylenepentamine and 2-aminopyrrole is 1: 2.
and (3) introducing argon into the oxygen-free atmosphere in the step (3) during the polymerization reaction.
The invention has the advantages and effects that:
the invention combines the imprinting technology, the layer-by-layer self-assembly method and the drop coating method, successfully develops a Faviravir molecular imprinting paste electrode sensor by doping a gallium phosphide paste electrode at a carbon quantum dot, and combines the imprinting technology, the layer-by-layer self-assembly method and the drop coating methodCompared with the response of the carbon quantum dot doped molecular imprinting electrode, the Faviravir molecular imprinting paste electrode sensor prepared by the invention has greatly improved response, good selectivity and high sensitivity, shows higher affinity and selectivity to Faviravir, and has the response current and the concentration of the Faviravir of 2.0 × 10-8~8.0×10-6Has good linear relation in the mol/L range and the detection limit is 8.32 × 10-8The Faviravir molecularly imprinted paste electrode sensor prepared by the invention is successfully used for detecting Faviravir in a sample at a recovery rate of 95.12-104.56 mol/L, so that the Faviravir molecularly imprinted paste electrode sensor prepared by the invention can be widely applied to related fields of medicine, biology, environmental protection detection and the like.
The prepared favelavir molecularly imprinted paste electrode sensor does not use toxic reagents in the preparation process, is environment-friendly and green, and has a simple preparation process.
Detailed Description
Example 1
(1) Preparing a gallium phosphide/quantum dot paste electrode: in an agate mortar, adding nano gallium phosphide: 46g, graphene oxide: 18g, 1-ethylmethyl ether-3-methylimidazole phosphate: 14g, carbon quantum dot: 5g, glycerol: 10g, ethanol: 9 mL, and grinding uniformly to obtain a mixture carbon paste; then the carbon paste is put into a glass tube which is connected with a lead and has an inner diameter of phi 5mm, and the glass tube is compacted, dried, polished by polishing powder, polished and washed by deionized water, thus obtaining the gallium phosphide/quantum dot paste electrode;
(2) preparing a gamma-glycidoxypropyltrimethoxysilane modified gallium phosphide/quantum dot paste electrode: in the reactor, ethanol: 91 mL, γ -glycidoxypropyltrimethoxysilane: 28g, stirring and dissolving, placing the polished gallium nitride paste electrode, soaking at room temperature for 4h, heating to 55 +/-2 ℃, reacting at constant temperature for 4h, taking out the electrode, washing with absolute ethyl alcohol, and drying to obtain the gamma-glycidyl ether oxypropyl trimethoxysilane modified gallium phosphide/quantum dot paste electrode;
(3) preparation of a Faviravir molecularly imprinted polymer: in the reactor, ethanol: 86 mL, tetraethylenepentamine: 10g, 2-aminopyrrole: 8g, faviravir: 6g, trimethylolpropane triglycidyl ether: 6g, azobisisoheptonitrile: 2g, stirring and dissolving, introducing nitrogen to remove oxygen for 15min, stirring and reacting for 2-4 h at 55 +/-2 ℃, performing solid-liquid separation, and adding alcohol into the obtained product: soaking the mixed solution with the volume ratio of acetic acid being 8:1 for 8 hours, washing for multiple times, removing template molecules, and drying to obtain a faviravir molecularly imprinted polymer;
(4) preparing a Faviravir molecularly imprinted paste electrode sensor: in the reactor, N-dimethylformamide: 10mL, vinyl resin: 0.1g, faviravir molecularly imprinted polymer: 0.3g, heating and dissolving to prepare a favelavir molecularly imprinted polymer modification solution; and (3) dropwise adding 25 mu L of the solution into the gamma-glycidoxypropyltrimethoxysilane modified gallium phosphide/quantum dot paste electrode prepared in the step (2), placing the electrode under an infrared lamp, and volatilizing a dry solvent to obtain the Faviravir molecularly imprinted paste electrode sensor.
Example 2
(1) Preparing a gallium phosphide/quantum dot paste electrode: in an agate mortar, adding nano gallium phosphide: 22g, graphene oxide: 10g, 1-ethylmethyl ether-3-methylimidazole phosphate: 8g, carbon quantum dot: 2g, glycerol: 4g, ethanol: 5 mL, and grinding uniformly to obtain a mixture carbon paste; then the carbon paste is put into a glass tube which is connected with a lead and has an inner diameter of phi 5mm, and the glass tube is compacted, dried, polished by polishing powder, polished and washed by deionized water, thus obtaining the gallium phosphide/quantum dot paste electrode;
(2) preparing a gamma-glycidoxypropyltrimethoxysilane modified gallium phosphide/quantum dot paste electrode: in the reactor, ethanol: 47 mL, γ -glycidoxypropyltrimethoxysilane: 13g, stirring and dissolving, placing the polished gallium nitride paste electrode, soaking at room temperature for 4h, heating to 55 +/-2 ℃, reacting at constant temperature for 4h, taking out the electrode, washing with absolute ethyl alcohol, and drying to obtain the gamma-glycidyl ether oxypropyl trimethoxysilane modified gallium phosphide/quantum dot paste electrode;
(3) preparation of a Faviravir molecularly imprinted polymer: in the reactor, ethanol: 44 mL, tetraethylenepentamine: 6g, 2-aminopyrrole: 5g, faviravir: 2g, trimethylolpropane triglycidyl ether: 2g, azobisisoheptonitrile: 0.5g, stirring and dissolving, introducing nitrogen to remove oxygen for 15min, stirring and reacting for 2-4 h at 55 +/-2 ℃ in an oxygen-free atmosphere, carrying out solid-liquid separation, and adding alcohol into the obtained product: soaking the mixed solution with the volume ratio of acetic acid being 8:1 for 8 hours, washing for multiple times, removing template molecules, and drying to obtain a faviravir molecularly imprinted polymer;
(4) preparing a Faviravir molecularly imprinted paste electrode sensor: in the reactor, N-dimethylformamide: 11mL, vinyl resin: 0.1g, faviravir molecularly imprinted polymer: 0.2g, heating and dissolving to prepare a favelavir molecularly imprinted polymer modification solution; and (3) dropwise adding 30 mu L of the solution into the gamma-glycidoxypropyltrimethoxysilane modified gallium phosphide/quantum dot paste electrode prepared in the step (2), placing the electrode under an infrared lamp, and volatilizing a dry solvent to obtain the Faviravir molecularly imprinted paste electrode sensor.
Example 3
(1) Preparing a gallium phosphide/quantum dot paste electrode: in an agate mortar, adding nano gallium phosphide: 48g, graphene oxide: 16g, 1-ethylmethyl ether-3-methylimidazole phosphate: 12g, carbon quantum dot: 6g, glycerol: 12g, ethanol: 8 mL, and grinding uniformly to obtain a mixture carbon paste; then the carbon paste is put into a glass tube which is connected with a lead and has an inner diameter of phi 5mm, and the glass tube is compacted, dried, polished by polishing powder, polished and washed by deionized water, thus obtaining the gallium phosphide/quantum dot paste electrode;
(2) preparing a gamma-glycidoxypropyltrimethoxysilane modified gallium phosphide/quantum dot paste electrode: in the reactor, ethanol: 88 mL, γ -glycidoxypropyltrimethoxysilane: 30g, stirring and dissolving, placing the polished gallium nitride paste electrode, soaking at room temperature for 4h, heating to 55 +/-2 ℃, reacting at constant temperature for 4h, taking out the electrode, washing with absolute ethyl alcohol, and drying to obtain the gamma-glycidyl ether oxypropyl trimethoxysilane modified gallium phosphide/quantum dot paste electrode;
(3) preparation of a Faviravir molecularly imprinted polymer: in the reactor, ethanol: 45 mL, tetraethylenepentamine: 4g, 2-aminopyrrole: 3g, faviravir: 4g, trimethylolpropane triglycidyl ether: 5g, azobisisoheptonitrile: 0.8g, stirring and dissolving, introducing nitrogen to remove oxygen for 15min, stirring and reacting for 2-4 h at 55 +/-2 ℃ in an oxygen-free atmosphere, carrying out solid-liquid separation, and adding alcohol into the obtained product: soaking the mixed solution with the volume ratio of acetic acid being 8:1 for 8 hours, washing for multiple times, removing template molecules, and drying to obtain a faviravir molecularly imprinted polymer;
(4) preparing a Faviravir molecularly imprinted paste electrode sensor: in the reactor, N-dimethylformamide: 10mL, vinyl resin: 0.3g, faviravir molecularly imprinted polymer: 0.2g, heating and dissolving to prepare a favelavir molecularly imprinted polymer modification solution; and (3) dropwise adding 20 mu L of the solution into the gamma-glycidoxypropyltrimethoxysilane modified gallium phosphide/quantum dot paste electrode prepared in the step (2), placing the electrode under an infrared lamp, and volatilizing a dry solvent to obtain the Faviravir molecularly imprinted paste electrode sensor.
Example 4
(1) Preparing a gallium phosphide/quantum dot paste electrode: in an agate mortar, adding nano gallium phosphide: 23g, graphene oxide: 9g, 1-Ethylmethyl ether-3-methylimidazole phosphate: 6g, carbon quantum dot: 2g, glycerol: 6g, ethanol: 7 mL, and grinding uniformly to obtain a mixture carbon paste; then the carbon paste is put into a glass tube which is connected with a lead and has an inner diameter of phi 5mm, and the glass tube is compacted, dried, polished by polishing powder, polished and washed by deionized water, thus obtaining the gallium phosphide/quantum dot paste electrode;
(2) preparing a gamma-glycidoxypropyltrimethoxysilane modified gallium phosphide/quantum dot paste electrode: in the reactor, ethanol: 91 mL, γ -glycidoxypropyltrimethoxysilane: 28g, stirring and dissolving, placing the polished gallium nitride paste electrode, soaking at room temperature for 4h, heating to 55 +/-2 ℃, reacting at constant temperature for 4h, taking out the electrode, washing with absolute ethyl alcohol, and drying to obtain the gamma-glycidyl ether oxypropyl trimethoxysilane modified gallium phosphide/quantum dot paste electrode;
(3) preparation of a Faviravir molecularly imprinted polymer: in the reactor, ethanol: 42 mL, tetraethylenepentamine: 6g, 2-aminopyrrole: 4g, faviravir: 3g, trimethylolpropane triglycidyl ether: 4g, azobisisoheptonitrile: 2g, stirring and dissolving, introducing nitrogen to remove oxygen for 15min, stirring and reacting for 2-4 h at 55 +/-2 ℃, performing solid-liquid separation, and adding alcohol into the obtained product: soaking the mixed solution with the volume ratio of acetic acid being 8:1 for 8 hours, washing for multiple times, removing template molecules, and drying to obtain a faviravir molecularly imprinted polymer;
(4) preparing a Faviravir molecularly imprinted paste electrode sensor: in the reactor, N-dimethylformamide: 10mL, vinyl resin: 0.2g, faviravir molecularly imprinted polymer: 0.1g, heating and dissolving to prepare a favelavir molecularly imprinted polymer modification solution; and (3) dropwise adding 28 mu L of the solution into the gamma-glycidoxypropyltrimethoxysilane modified gallium phosphide/quantum dot paste electrode prepared in the step (2), placing the electrode under an infrared lamp, and volatilizing a dry solvent to obtain the Faviravir molecularly imprinted paste electrode sensor.
Example 5
The Faviravir molecularly imprinted paste electrode sensor prepared in the embodiment 1-4 is used for detection of Faviravir, and comprises the following steps:
(1) preparing a standard solution: preparing a group of Vivira standard solutions with different concentrations including blank standard samples, wherein the base solution is phosphate buffer solution with the pH value of 7.0;
(2) drawing a working curve: Ag/AgCl is used as a reference electrode, a platinum wire electrode is used as an auxiliary electrode, the Faviravir molecular imprinting paste electrode sensor prepared by the invention is used as a working electrode to form a three-electrode system, and the three-electrode system is connected with a CHI660B electrochemical workstation at K3[Fe(CN)6]In the solution, the cyclic voltammetry is adopted to carry out detection in the potential range of-1.0-1.2V, and the response current of the blank standard sample is recorded asI 0 The response current of the favelawir standard solution with different concentrations isI i The difference of the decrease of the response current is△ I=I 0 -I i ,△IAnd favelawei markMass concentration of quasi-solutioncAre in linear relation with each other, and are drawn△I ~cA working curve;
(3) detection of Favilavir: replacing the standard Faviravir solution in the step (1) with a sample to be detected, detecting according to the method in the step (2), and detecting according to the difference value of the reduction of the response current△IAnd working curve to obtain the content of the favilavir in the sample to be detected;
said K3[Fe(CN)6]The concentration of the solution is 4.0 mmol/L;
the concentration of the phosphate buffer solution with the pH value of 7.0 is 0.05 mol/L.
Claims (5)
1. A preparation method of a Faviravir molecularly imprinted paste electrode sensor is characterized by comprising the following process steps:
(1) preparing a gallium phosphide/quantum dot paste electrode: adding the nano gallium phosphide into an agate mortar according to the following mass percentages: 44-48%, graphene oxide: 16-20%, 1-ethyl methyl ether-3-methylimidazole phosphate: 12-16%, carbon quantum dots: 4-6%, glycerin: 8-12%, ethanol: 6-10%, wherein the sum of the mass percentages of the components is one hundred percent, and the mixture is uniformly ground to obtain a mixture carbon paste; then the carbon paste is put into a glass tube which is connected with a lead and has an inner diameter of phi 5mm, and the glass tube is compacted, dried, polished by polishing powder, polished and washed by deionized water, thus obtaining the gallium phosphide/quantum dot paste electrode;
(2) preparing a gamma-glycidoxypropyltrimethoxysilane modified gallium phosphide/quantum dot paste electrode: adding ethanol into a reactor according to the mass percentage concentration of the following components: 70-74% of gamma-glycidyl ether oxypropyl trimethoxysilane: 26-30%, wherein the sum of the mass percentages of the components is one hundred percent, stirring and dissolving, putting the polished gallium nitride paste electrode into the electrode, soaking the electrode at room temperature for 4 hours, heating the electrode to 55 +/-2 ℃, reacting at constant temperature for 4 hours, taking out the electrode, washing the electrode with absolute ethyl alcohol, and drying to obtain the gamma-glycidyl ether oxypropyl trimethoxysilane modified gallium phosphide/quantum dot paste electrode;
(3) preparation of a Faviravir molecularly imprinted polymer: adding ethanol into a reactor according to the following composition mass percentage: 66-72%, tetraethylenepentamine: 8-12%, 2-aminopyrrole: 6-10%, favilavir: 4-8%, trimethylolpropane triglycidyl ether: 4-8%, azobisisoheptonitrile: 1.0-3.0%, the sum of the contents of all the components is one hundred percent, stirring and dissolving, introducing argon to remove oxygen for 15min, carrying out anaerobic atmosphere, stirring and reacting at 55 +/-2 ℃ for 2-4 h, carrying out solid-liquid separation, and adding alcohol into the obtained product: soaking the mixed solution with the volume ratio of acetic acid being 8:1 for 8 hours, washing for multiple times, removing template molecules, and drying to obtain a faviravir molecularly imprinted polymer;
(4) preparing a Faviravir molecularly imprinted paste electrode sensor: adding N, N-dimethylformamide into a reactor according to the mass percentage concentration of the following components: 95-97%, vinyl resin: 1.0-3.0%, Faviravir molecularly imprinted polymer: 1.0-3.0%, wherein the sum of the contents of all the components is one hundred percent, and heating and dissolving to prepare a faviravir molecularly imprinted polymer modification solution; and (3) dropwise adding 20-30 mu L of the solution into the gamma-glycidoxypropyltrimethoxysilane modified gallium phosphide/quantum dot paste electrode prepared in the step (2), placing the electrode under an infrared lamp, and volatilizing a dry solvent to obtain the Faviravir molecularly imprinted paste electrode sensor.
2. The method for preparing the faviravir molecularly imprinted paste electrode sensor according to claim 1, wherein the carbon quantum dots in step (1) are oil-soluble carbon quantum dots.
3. The method for preparing the faviravir molecularly imprinted paste electrode sensor according to claim 1, wherein the molar ratio of the tetraethylenepentamine to the 2-aminopyrrole in step (3) is 1: 2.
4. the method for preparing the faviravir molecularly imprinted paste electrode sensor according to claim 1, wherein argon is continuously introduced into the oxygen-free atmosphere in the step (3) during the polymerization reaction.
5. The faviravir molecularly imprinted paste electrode sensor prepared by the method according to claim 1.
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