CN107037098A - A kind of preparation method for being used to detect Kaempferol molecular engram sensor - Google Patents
A kind of preparation method for being used to detect Kaempferol molecular engram sensor Download PDFInfo
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Abstract
The invention discloses a kind of preparation method for being used to detect Kaempferol molecular engram sensor, it is characterised by, first using gold chloride：Glass-carbon electrode is modified with graphene oxide, nanogold graphene modified glass-carbon electrode is obtained；Then, added in reactor, ethanol：44 ~ 50%, GDMA：10 ~ 14%, styrene：The butyl hexafluorophosphate of 5 ~ 8%, 1 vinyl 3：30 ~ 35%, azodiisobutyronitrile：1.0 ~ 2.0%, Kaempferide：2.0 ~ 5.0%, stirring and dissolving, anaerobic atmosphere, 55 ± 2 DEG C of 28 ~ 30h of stirring reaction, using methanol：Acetic acid mixed solution removes template molecule and produces Kaempferol molecularly imprinted polymer；Again by molecularly imprinted polymer drop coating to nanogold graphene modified glass-carbon electrode, Kaempferol molecular engram sensor is obtained.The sensor has high compatibility and selectivity.With sensitivity height, specificity is good, detect quick, can Reusability.
Description
Technical field
The present invention relates to a kind of preparation method of molecular engram sensor and quick detection applied technical field, especially
It is related to a kind of preparation method for being used to detect Kaempferol molecular engram sensor, is specifically made based on molecular engram specific recognition
With for detecting the Kaempferol in medicine, food, biological sample.
Background technology
Kaempferide（Scientific name is 3,5,7- trihydroxy -4'- methoxy flavones）Its molecular formula is C16H12O6, relative molecular mass
It is a kind of bioflavonoid for having a special molecular structure, with a variety of pharmacological activity for 300.26.Kaempferide is yellow crystal
Shape powder, is slightly soluble in water, is dissolved in hot ethanol, ether and alkali.Kaempferol can activate melan-a melanocyte tyrosinases
Activity, promote melanocyte proliferation, make melanin genesis amount increase etc. effect, with anticancer, suppress fertility, anti-epileptic, anti-inflammatory,
Antioxidant, spasmolysis, antiulcer, cholagogic diuretics, cough-relieving.Oxygen radical toxicity be induce a variety of diseases major reason it
One, the generation of anoxic, aging, the artery sclerosis even disease such as tumour is relevant with the toxicity of oxygen radical.Kaempferol has very
Strong removal oxygen radical ability.Kaempferide also has very strong antivirus action
At present, the method for detection Kaempferide mainly has high performance liquid chromatography, a liquid chromatography-mass spectrography, the chromatographic degree of accuracy by
One definite limitation and instrument is somewhat expensive needs professional to operate, also limit its application.Further, since Kaempferide with it is other
It is difficult to separate that flavonoids molecular structure is very close, is interfered with each other during measure, and accurate detection Kaempferide is highly difficult.Therefore, find
A kind of selectivity is good, sensitivity is high, the method for the detection Kaempferide easy to operate being used for is critically important.
Molecular imprinting technology is one of the current main method of high selectivity material of exploitation with molecular recognition function, it
It is by forming a highly cross-linked rigid macromolecule around template molecule, removing after template molecule in molecular engram polymerization
The recognition site with binding ability is left in the network structure of thing, one kind of high selection recognition performance is shown to template molecule
Technology.This technology imitates precordainment with its structure and specific recognition is increasingly paid close attention to by people, has been used successfully to solid phase
Extraction or micro- SPE, the field such as affinity chromatography or Capillary Electrophoresis and sensor.
The molecular engram sensor prepared according to this technology, applied to Pharmaceutical Analysis, environmental protection and life science
In play a very important role.Functional molecular is modified on electrode in a suitable manner, prepare selectivity good, sensitivity it is high,
There is the reproducible electrochemical sensor of certain service life to turn into the problem that analysis science worker makes great efforts to explore.But it is traditional
Trace film thickness prepared by immunoblot method is difficult to control to, and high-crosslinking-degree causes electron transmission speed and low-response, Monitoring lower-cut
High and regeneration and poorly reversible, application of the influence molecular imprinting technology in electrochemical sensor.Therefore, a kind of spirit is set up
The detection method that quick, quick, easy, specific high, reproducible economy is used, to researcher, manufacturing enterprise, Quality Control people
Member, import and export commodity inspection, government administration section etc. in the urgent need to, it is accurate to the Kaempferia galanga cellulose content in food, medicine, biological sample
Really quantitative determination is very necessary, also has great importance for Kaempferide production and pharmacological research.
The content of the invention
The purpose of the present invention is that molecular engram is combined to there is provided a kind of Kaempferide molecular engram with electrochemical sensor
The preparation method of sensor, mainly using Kaempferide as template, graphene oxide, nano Au particle are passed through in glassy carbon electrode surface
Modification, improve the sensitivity of sensor, Kaempferide molecular engram sensor prepared using drop coating legal system.
Instrument and reagent
CHI660B electrochemical workstations（Shanghai Chen Hua instrument company）, test and use three-electrode system：Platinum electrode is auxiliary electricity
Pole, Ag/AgCl is reference electrode（SCE）, glass-carbon electrode（GCE）For working electrode；KQ-250E type ultrasonic cleaners（Female peak
Ultrasonic instrument Co., Ltd）.
Graphene oxide；GDMA, styrene；1- vinyl -3- butyl hexafluorophosphates, azo
Bis-isobutyronitrile, absolute ethyl alcohol；Kaempferide；Gold chloride；Animal glue；Phosphate buffer solution；Agents useful for same is that analysis is pure, and experiment is used
Water is redistilled water.
The purpose of the present invention is achieved through the following technical solutions.
A kind of preparation method for being used to detect Kaempferol molecular engram sensor, is characterised by, this method has following work
Skill step：
（1）It is prepared by nanogold graphene modified liquid：In the reactor, added by following composition mass percentage concentration, deionized water：
90 ~ 95%, gold chloride：1.0 ~ 3.0%, graphene oxide：2.5 ~ 8%, each component content sum is a hundred per cent, ultrasonic at room temperature
15min, is uniformly dispersed, and obtains nanogold graphene modified liquid；
（2）It is prepared by nanogold graphene modified glass-carbon electrode：Glass-carbon electrode is subjected to table with 0.2 μm, 0.01 μm of polishing powder successively
Mirror polish, is then cleaned by ultrasonic with redistilled water respectively, ethanol washing, drying, and 18 ~ 20 μ L, which are added dropwise, in glassy carbon electrode surface receives
Rice gold-graphene decorating liquid, is placed under infrared lamp, volatilizees after dry solvent, produces nanogold graphene modified glass-carbon electrode；
（3）The preparation of Kaempferol molecularly imprinted polymer：In the reactor, added by following composition mass percentage concentration, ethanol：
44 ~ 50%, GDMA：10 ~ 14%, styrene：5 ~ 8%, 1- vinyl -3- butyl hexafluorophosphates：30~
35%, azodiisobutyronitrile：1.0 ~ 2.0%, Kaempferide：2.0 ~ 5.0%, each component content sum is absolutely stirring and dissolving,
The logical min of inert gas deoxygenation 10, anaerobic atmosphere, 55 ± 2 DEG C of 28 ~ 30h of stirring reaction, by obtained product methanol：Acetic acid body
Product is than being 10:1 mixed solution soaks 12 ~ 15h, removes template molecule, dries, produces Kaempferol molecularly imprinted polymer；
（4）The preparation method of Kaempferol molecular engram sensor：Appropriate Kaempferol molecularly imprinted polymer is taken to be scattered in 1%
In gelatin solution, 22g/L Kaempferol imprinted polymer solution is made；Then the above-mentioned μ L of solution 12 ~ 15 are added drop-wise to
Step（2）The nanogold graphene modified glass-carbon electrode of preparation, is placed under infrared lamp, after the dry solvent that volatilizees, produces Kaempferol point
Sub- trace sensor.
In step（1）Described in gold chloride and graphene oxide mass ratio be 1：2.5 ~ 3.0 is optimal.
In step（3）Described in GDMA and styrene and 1- vinyl -3- butyl hexafluoro phosphorus
Hydrochlorate mol ratio is 1：1：1 is optimal.
In step（3）Described in inert gas be neon or argon gas.
In step（3）Described in anaerobic atmosphere be passed through inert gas always for polymerization process.
Advantages of the present invention and effect are：
Engram technology, LBL self-assembly method and drop-coating are combined by the present invention, in graphene oxide and decorated by nano-gold glass carbon
Electrode surface successfully have developed a kind of Kaempferol trace electrochemical sensor with specific selectivity.By with anaerobic fossil
The response of that of the molecular engram electrode of black alkene decorated by nano-gold is compared, Kaempferol molecular engram sensing prepared by the present invention
The response of device is greatly improved.The trace sensor shows higher compatibility and selectivity, response current and mountain to Kaempferol
How the concentration of element is 1.0 × 10-7~2.0×10-4It is in good linear relationship in the range of mol/L, detection is limited to 4.21 × 10- 8Kaempferol molecular engram sensor prepared by the present invention is used successfully to Kaempferol in medicine, food, biological sample by mol/L
In detection, the rate of recovery is between 95.32 ~ 104.26%, therefore molecular engram can be widely applied to of sensor prepared by the present invention
The association areas such as work, biological medicine, food, environmental protection tests.
Embodiment
Embodiment 1
（1）It is prepared by nanogold graphene modified liquid：In the reactor, it is separately added into, deionized water：92mL, gold chloride：2g, oxygen
Graphite alkene：6g, ultrasound 15min, is uniformly dispersed at room temperature, obtains nanogold graphene modified liquid；
（2）It is prepared by nanogold graphene modified glass-carbon electrode：Glass-carbon electrode is subjected to table with 0.2 μm, 0.01 μm of polishing powder successively
Mirror polish, is then cleaned by ultrasonic with redistilled water respectively, and L nanometers of 19 μ is added dropwise in glassy carbon electrode surface in ethanol washing, drying
Gold-graphene decorating liquid, is placed under infrared lamp, volatilizees after dry solvent, produces nanogold graphene modified glass-carbon electrode；
（3）The preparation of Kaempferol molecularly imprinted polymer：In the reactor, it is separately added into, ethanol：60 mL, dimethacrylate
Glycol ester：12g, styrene：6g, 1- vinyl -3- butyl hexafluorophosphates：32g, azodiisobutyronitrile：1g, Kaempferide：
2g, stirring and dissolving leads to the min of inert gas deoxygenation 10, anaerobic atmosphere, 55 ± 2 DEG C of stirring reaction 29h, by obtained product first
Alcohol：Acetic acid volume ratio is 10:1 mixed solution soaks 13h, removes template molecule, dries, and produces the polymerization of Kaempferol molecular engram
Thing；
（4）The preparation method of Kaempferol molecular engram sensor：Appropriate Kaempferol molecularly imprinted polymer is taken to be scattered in 1%
In gelatin solution, 22g/L Kaempferol imprinted polymer solution is made；Then the above-mentioned μ L of solution 13 are added drop-wise to step
Suddenly（2）The nanogold graphene modified glass-carbon electrode of preparation, is placed under infrared lamp, volatilizees after dry solvent, produces Kaempferol molecule
Trace sensor.
Embodiment 2
（1）It is prepared by nanogold graphene modified liquid：In the reactor, it is separately added into, deionized water：9.5mL, gold chloride：0.1g,
Graphene oxide：0.4g, ultrasound 15min, is uniformly dispersed at room temperature, obtains nanogold graphene modified liquid；
（2）It is prepared by nanogold graphene modified glass-carbon electrode：Glass-carbon electrode is subjected to table with 0.2 μm, 0.01 μm of polishing powder successively
Mirror polish, is then cleaned by ultrasonic with redistilled water respectively, and L nanometers of 18 μ is added dropwise in glassy carbon electrode surface in ethanol washing, drying
Gold-graphene decorating liquid, is placed under infrared lamp, volatilizees after dry solvent, produces nanogold graphene modified glass-carbon electrode；
（3）The preparation of Kaempferol molecularly imprinted polymer：In the reactor, it is separately added into, ethanol：6.3 mL, dimethyl allene
Sour glycol ester：1.0g, styrene：0.5g, 1- vinyl -3- butyl hexafluorophosphates：3.0g, azodiisobutyronitrile：0.2g,
Kaempferide：0.3g, stirring and dissolving, the logical min of inert gas deoxygenation 10, anaerobic atmosphere, 55 ± 2 DEG C of stirring reaction 28h will be obtained
Product methanol：Acetic acid volume ratio is 10:1 mixed solution soaks 14h, removes template molecule, dries, produces Kaempferol molecule
Imprinted polymer；
（4）The preparation method of Kaempferol molecular engram sensor：Appropriate Kaempferol molecularly imprinted polymer is taken to be scattered in 1%
In gelatin solution, 22g/L Kaempferol imprinted polymer solution is made；Then the above-mentioned μ L of solution 14 are added drop-wise to step
Suddenly（2）The nanogold graphene modified glass-carbon electrode of preparation, is placed under infrared lamp, volatilizees after dry solvent, produces Kaempferol molecule
Trace sensor.
Embodiment 3
（1）It is prepared by nanogold graphene modified liquid：In the reactor, it is separately added into, deionized water：9mL, gold chloride：0.3g, oxygen
Graphite alkene：0.7g, ultrasound 15min, is uniformly dispersed at room temperature, obtains nanogold graphene modified liquid；
（2）It is prepared by nanogold graphene modified glass-carbon electrode：Glass-carbon electrode is subjected to table with 0.2 μm, 0.01 μm of polishing powder successively
Mirror polish, is then cleaned by ultrasonic with redistilled water respectively, and L nanometers of 20 μ is added dropwise in glassy carbon electrode surface in ethanol washing, drying
Gold-graphene decorating liquid, is placed under infrared lamp, volatilizees after dry solvent, produces nanogold graphene modified glass-carbon electrode；
（3）The preparation of Kaempferol molecularly imprinted polymer：In the reactor, it is separately added into, ethanol：5.6 mL, dimethyl allene
Sour glycol ester：1.3g, styrene：0.7g, 1- vinyl -3- butyl hexafluorophosphates：3.1g, azodiisobutyronitrile：0.1g,
Kaempferide：0.4g, stirring and dissolving, the logical min of inert gas deoxygenation 10, anaerobic atmosphere, 55 ± 2 DEG C of stirring reaction 30h will be obtained
Product methanol：Acetic acid volume ratio is 10:1 mixed solution soaks 12h, removes template molecule, dries, produces Kaempferol molecule
Imprinted polymer；
（4）The preparation method of Kaempferol molecular engram sensor：Appropriate Kaempferol molecularly imprinted polymer is taken to be scattered in 1%
In gelatin solution, 22g/L Kaempferol imprinted polymer solution is made；Then the above-mentioned μ L of solution 12 are added drop-wise to step
Suddenly（2）The nanogold graphene modified glass-carbon electrode of preparation, is placed under infrared lamp, volatilizees after dry solvent, produces Kaempferol molecule
Trace sensor.
Embodiment 4
（1）It is prepared by nanogold graphene modified liquid：In the reactor, it is separately added into, deionized water：9.4mL, gold chloride：0.2g,
Graphene oxide：0.5g, ultrasound 15min, is uniformly dispersed at room temperature, obtains nanogold graphene modified liquid；
（2）It is prepared by nanogold graphene modified glass-carbon electrode：Glass-carbon electrode is subjected to table with 0.2 μm, 0.01 μm of polishing powder successively
Mirror polish, is then cleaned by ultrasonic with redistilled water respectively, and L nanometers of 20 μ is added dropwise in glassy carbon electrode surface in ethanol washing, drying
Gold-graphene decorating liquid, is placed under infrared lamp, volatilizees after dry solvent, produces nanogold graphene modified glass-carbon electrode；
（3）The preparation of Kaempferol molecularly imprinted polymer：In the reactor, it is separately added into, ethanol：5.7 mL, dimethyl allene
Sour glycol ester：1.1g, styrene：0.5g, 1- vinyl -3- butyl hexafluorophosphates：3.5g, azodiisobutyronitrile：0.1g,
Kaempferide：0.5g, stirring and dissolving, the logical min of inert gas deoxygenation 10, anaerobic atmosphere, 55 ± 2 DEG C of stirring reaction 30h will be obtained
Product methanol：Acetic acid volume ratio is 10:1 mixed solution soaks 15h, removes template molecule, dries, produces Kaempferol molecule
Imprinted polymer；
（4）The preparation method of Kaempferol molecular engram sensor：Appropriate Kaempferol molecularly imprinted polymer is taken to be scattered in 1%
In gelatin solution, 22g/L Kaempferol imprinted polymer solution is made；Then the above-mentioned μ L of solution 15 are added drop-wise to step
Suddenly（2）The nanogold graphene modified glass-carbon electrode of preparation, is placed under infrared lamp, volatilizees after dry solvent, produces Kaempferol molecule
Trace sensor.
Embodiment 5
By the Kaempferol molecular engram sensor prepared by above-described embodiment 1 ~ 4, for the detection of Kaempferol, step is as follows：
（1）Standard liquid is prepared：The Kaempferol standard liquid of one group of various concentrations including blank standard specimen is prepared, bottom liquid is
PH7.0 phosphate buffer solution；
（2）Working curve is drawn：It is reference electrode by Ag/AgCl, platinum electrode is auxiliary electrode, electrode prepared by the present invention is
Working electrode constitutes three-electrode system, CHI660B electrochemical workstations is connected, in K3[Fe(CN)6] in solution, lied prostrate using circulation
Peace method is detected that the response current of blank standard specimen is designated as in -0.20 ~ 1.0V potential rangesI 0 , the mountain containing various concentrations
How the response current of plain standard liquid isI i , response current reduction difference be△I=I 0 -I i ,△IIt is molten with Kaempferol standard
The mass concentration of liquidcBetween it is linear, draw△I ~cWorking curve；
（3）The detection of Kaempferol：Step is replaced with testing sample（1）In Kaempferol standard liquid, according to step（2）Method
Detected, the difference reduced according to response current△IAnd working curve, obtain the content of Kaempferol in testing sample；
The K3[Fe(CN)6] solution concentration be 10mmol/L；
The concentration of the phosphate buffer solution of the pH7.0 is in 80m mol/L.
Claims (6)
1. a kind of preparation method for being used to detect Kaempferol molecular engram sensor, is characterised by, this method has following technique
Step：
（1）It is prepared by nanogold graphene modified liquid：In the reactor, added by following composition mass percentage concentration, deionized water：
90 ~ 95%, gold chloride：1.0 ~ 3.0%, graphene oxide：2.5 ~ 8%, each component content sum is a hundred per cent, ultrasonic at room temperature
15min, is uniformly dispersed, and obtains nanogold graphene modified liquid；
（2）It is prepared by nanogold graphene modified glass-carbon electrode：Glass-carbon electrode is subjected to table with 0.2 μm, 0.01 μm of polishing powder successively
Mirror polish, is then cleaned by ultrasonic with redistilled water respectively, ethanol washing, drying, and 18 ~ 20 μ L, which are added dropwise, in glassy carbon electrode surface receives
Rice gold-graphene decorating liquid, is placed under infrared lamp, volatilizees after dry solvent, produces nanogold graphene modified glass-carbon electrode；
（3）The preparation of Kaempferol molecularly imprinted polymer：In the reactor, added by following composition mass percentage concentration, ethanol：
44 ~ 50%, GDMA：10 ~ 14%, styrene：5 ~ 8%, 1- vinyl -3- butyl hexafluorophosphates：30~
35%, azodiisobutyronitrile：1.0 ~ 2.0%, Kaempferide：2.0 ~ 5.0%, each component content sum is absolutely stirring and dissolving,
The logical min of inert gas deoxygenation 10, anaerobic atmosphere, 55 ± 2 DEG C of 28 ~ 30h of stirring reaction, by obtained product methanol：Acetic acid body
Product is than being 10:1 mixed solution soaks 12 ~ 15h, removes template molecule, dries, produces Kaempferol molecularly imprinted polymer；
（4）The preparation method of Kaempferol molecular engram sensor：Appropriate Kaempferol molecularly imprinted polymer is taken to be scattered in 1%
In gelatin solution, 22g/L Kaempferol imprinted polymer solution is made；Then the above-mentioned μ L of solution 12 ~ 15 are added drop-wise to
Step（2）The nanogold graphene modified glass-carbon electrode of preparation, is placed under infrared lamp, after the dry solvent that volatilizees, produces Kaempferol point
Sub- trace sensor.
2. a kind of preparation method for being used to detect Kaempferol molecular engram sensor according to claim 1, is characterised by,
Step（1）Described in gold chloride and graphene oxide mass ratio be 1：2.5 ~ 3.0 is optimal.
3. a kind of preparation method for being used to detect Kaempferol molecular engram sensor according to claim 1, is characterised by,
Step（3）Described in GDMA and styrene and 1- vinyl -3- butyl hexafluorophosphate mol ratios
For 1：1：1 is optimal.
4. a kind of preparation method for being used to detect Kaempferol molecular engram sensor according to claim 1, is characterised by,
Step（3）Described in inert gas be neon or argon gas or Krypton.
5. a kind of preparation method for being used to detect Kaempferol molecular engram sensor according to claim 1, is characterised by,
Step（3）Described in anaerobic atmosphere be passed through inert gas always for polymerization process.
6. a kind of mountain for detecting prepared by the preparation method of Kaempferol molecular engram sensor according to claim 1
How plain molecular engram sensor.
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| CN108760849A (en) * | 2018-06-19 | 2018-11-06 | 孙大明 | A kind of preparation method of the molecularly imprinted polymer modified electrode of quick detection Ofloxacin |
| CN109001285A (en) * | 2018-08-29 | 2018-12-14 | 济南大学 | A kind of preparation method of prometryn molecular engram sensor |
| CN109060910A (en) * | 2018-08-29 | 2018-12-21 | 济南大学 | A kind of preparation method of isopropyl methoxalamine molecular engram sensor |
| CN109142498A (en) * | 2018-08-29 | 2019-01-04 | 济南大学 | A kind of preparation method of paraquat molecular engram sensor |
| CN111551617A (en) * | 2020-04-09 | 2020-08-18 | 济南大学 | Preparation method of faviravir molecularly imprinted paste electrode sensor |
| CN111551618A (en) * | 2020-04-09 | 2020-08-18 | 济南大学 | Preparation method of sirolimus molecularly imprinted erbium nitride composite paste electrode |
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| CN107824220A (en) * | 2017-11-09 | 2018-03-23 | 东南大学 | The preparation method of golden nanometer particle graphene melamine sponge composite |
| CN108760849A (en) * | 2018-06-19 | 2018-11-06 | 孙大明 | A kind of preparation method of the molecularly imprinted polymer modified electrode of quick detection Ofloxacin |
| CN109001285A (en) * | 2018-08-29 | 2018-12-14 | 济南大学 | A kind of preparation method of prometryn molecular engram sensor |
| CN109060910A (en) * | 2018-08-29 | 2018-12-21 | 济南大学 | A kind of preparation method of isopropyl methoxalamine molecular engram sensor |
| CN109142498A (en) * | 2018-08-29 | 2019-01-04 | 济南大学 | A kind of preparation method of paraquat molecular engram sensor |
| CN109142498B (en) * | 2018-08-29 | 2020-09-04 | 济南大学 | Preparation method of paraquat molecular imprinting sensor |
| CN111551617A (en) * | 2020-04-09 | 2020-08-18 | 济南大学 | Preparation method of faviravir molecularly imprinted paste electrode sensor |
| CN111551618A (en) * | 2020-04-09 | 2020-08-18 | 济南大学 | Preparation method of sirolimus molecularly imprinted erbium nitride composite paste electrode |
| CN111551618B (en) * | 2020-04-09 | 2022-05-10 | 济南大学 | Preparation method of sirolimus molecularly imprinted erbium nitride composite paste electrode |
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