CN111549086A - Preparation method for preparing corn peptide by heat treatment-assisted enzyme method - Google Patents
Preparation method for preparing corn peptide by heat treatment-assisted enzyme method Download PDFInfo
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- CN111549086A CN111549086A CN202010423050.6A CN202010423050A CN111549086A CN 111549086 A CN111549086 A CN 111549086A CN 202010423050 A CN202010423050 A CN 202010423050A CN 111549086 A CN111549086 A CN 111549086A
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Abstract
A preparation method for preparing corn peptide by heat treatment assisted enzyme method comprises the following steps: the method comprises the following steps: pulverizing and refining the crude corn protein powder, adding distilled water into the pulverized corn protein powder, and fully and uniformly mixing to obtain a corn protein powder solution; step two: heating the corn protein powder solution obtained in the step one to 90-120 ℃, and keeping the temperature for 20-40 min; step three: after the heating in the second step is finished, cooling to 50-60 ℃, adding protease, and carrying out enzymolysis reaction for 4-8h under the stirring condition; step four: and (3) drying the mixed solution subjected to the enzymolysis reaction in the third step at the temperature of 125-150 ℃ for 2-3h to obtain the corn peptide product. The corn peptide obtained by the invention has the distilled water content of less than or equal to 5 percent, the crude protein content of more than or equal to 55 percent and the small peptide content of less than 1000Da of molecular weight of more than or equal to 90 percent. The corn peptide yield is improved, and the quality of the corn peptide can be ensured.
Description
Technical Field
The invention belongs to the field of preparation of corn peptides, and particularly relates to a preparation method for preparing corn peptides by using a heat treatment-assisted enzyme method and a preparation method thereof.
Background
The corn protein powder is commonly called as corn gluten meal, the protein content of the corn gluten meal is about 70 percent, and the corn peptide with small molecular weight can be obtained after the corn gluten meal is distilled and hydrolyzed by protease. The corn peptide has the functions of providing essential amino acid and nutrition for organisms, improving immunity, reducing blood pressure, improving digestion and absorption rate of the organisms and the like, and is easy to absorb. These functions and advantages make maize peptides a research hotspot.
Because the yield of the current method for producing the corn peptide is low and the content of the corn peptide protein is low, the corn protein powder is pretreated to improve the enzymolysis efficiency and the yield of the corn peptide. At present, common pretreatment modes include ultrasonic, microwave, high pressure and the like, but practice, use and research show that the conventional pretreatment modes have various problems, the obtained products have different quality, the obtained corn peptide has larger molecular weight, and a method which can improve the yield of the corn peptide and ensure the quality of the corn peptide products is lacked at present.
Disclosure of Invention
The invention provides a preparation method for preparing corn peptide by a heat treatment-assisted enzyme method, which is used for overcoming the defects in the prior art.
The invention is realized by the following technical scheme:
a preparation method for preparing corn peptide by heat treatment assisted enzyme method comprises the following steps:
the method comprises the following steps: pulverizing and refining the crude corn protein powder, adding distilled water into the pulverized corn protein powder, and fully and uniformly mixing to obtain a corn protein powder solution;
step two: heating the corn protein powder solution obtained in the step one to 90-120 ℃, and keeping the temperature for 20-40 min;
step three: after the heating in the second step is finished, cooling to 50-60 ℃, adding protease, and carrying out enzymolysis reaction for 4-8h under the stirring condition;
step four: and (3) drying the mixed solution subjected to the enzymolysis reaction in the third step at the temperature of 125-150 ℃ for 2-3h to obtain the corn peptide product.
The preparation method for preparing the corn peptide by the heat treatment-assisted enzyme method comprises the following steps of crushing and refining the crude corn protein powder, sieving the crushed crude corn protein powder with a 40-mesh sieve for later use, and continuously crushing the particles with the particle size larger than the 40-mesh sieve until all the particles pass through the 40-mesh sieve.
In the preparation method for preparing the corn peptide by the heat treatment-assisted enzyme method, the corn protein powder solid content of the corn protein powder solution obtained in the first step is 8-20%.
In the method for preparing corn peptide by using the heat treatment-assisted enzyme method, the protease in the third step is a mixture obtained by mixing any one or more of alkaline protease, neutral protease and flavourzyme in any proportion.
In the preparation method for preparing the corn peptide by the heat treatment-assisted enzyme method, the addition amount of the protease in the third step is 0.3-3.0% of the mass of the corn protein powder.
The invention has the advantages that: the corn peptide obtained by the invention has the distilled water content of less than or equal to 5 percent, the crude protein content of more than or equal to 55 percent and the small peptide content of less than 1000Da of molecular weight of more than or equal to 90 percent. The corn peptide yield is improved, and the quality of the corn peptide can be ensured; the corn protein powder is treated by heat treatment, and the heat treatment is green and environment-friendly, has no pollution and damage to the environment, and has the advantages of simple operation, economy, energy conservation and the like; meanwhile, the heat-treated zein can denature proteins through high temperature, and the secondary structure of the proteins, particularly disulfide bonds and hydrophobic and distilled water bonds, is changed, so that the combination of protease and the bottom is increased to a certain extent, and the effects of improving the yield of the zein and ensuring the quality are achieved.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The method comprises the following steps: weighing 100g of crude corn protein powder, crushing and refining, sieving with a 40-mesh sieve for later use, continuously crushing particles with the particle size larger than 40-mesh sieve until all particles pass through the 40-mesh sieve, adding the crushed corn protein powder into 733.3ml of distilled water, and fully and uniformly mixing to prepare a corn protein powder solution with the corn protein powder solid content of 12%;
step two: heating the corn protein powder solution obtained in the step one to 115 ℃, and keeping the temperature for 30 min;
step three: after the heating in the second step is finished, cooling to 55 ℃, adding 0.8g of alkaline protease, 0.8g of neutral protease and 0.8g of flavourzyme, and carrying out enzymolysis reaction for 7 hours under the stirring condition;
step four: and (3) drying the mixed solution subjected to the enzymolysis reaction in the third step at the temperature of 135 ℃ for 2.7 hours to obtain the corn peptide product.
Example 2
The method comprises the following steps: weighing 100g of crude corn protein powder, crushing and refining, sieving with a 40-mesh sieve for later use, continuously crushing particles with the particle size larger than 40-mesh sieve until all particles pass through the 40-mesh sieve, adding 566.7ml of distilled water into the crushed corn protein powder, and fully and uniformly mixing to prepare a corn protein powder solution with the corn protein powder solid content of 15%;
step two: heating the corn protein powder solution obtained in the step one to 115 ℃, and keeping the temperature for 40 min;
step three: after the heating in the second step is finished, cooling to 55 ℃, adding 0.8g of alkaline protease, 0.8g of neutral protease and 0.8g of flavourzyme, and carrying out enzymolysis reaction for 8 hours under the stirring condition;
step four: and (3) drying the mixed solution subjected to the enzymolysis reaction in the third step at the temperature of 135 ℃ for 2 hours to obtain the corn peptide product.
Example 3
The method comprises the following steps: weighing 100g of crude corn protein powder, crushing and refining, sieving with a 40-mesh sieve for later use, continuously crushing particles with the particle size larger than 40-mesh sieve until all particles pass through the 40-mesh sieve, adding 900ml of distilled water into the crushed corn protein powder, and fully and uniformly mixing to prepare a corn protein powder solution with the corn protein powder solid content of 10%;
step two: heating the corn protein powder solution obtained in the step one to 115 ℃, and keeping the temperature for 25 min;
step three: after the heating in the second step is finished, cooling to 55 ℃, adding 0.6g of alkaline protease, 0.6g of neutral protease and 0.6g of flavourzyme, and carrying out enzymolysis reaction for 7 hours under the stirring condition;
step four: and (3) drying the mixed solution subjected to the enzymolysis reaction in the third step at the temperature of 135 ℃ for 2.5 hours to obtain the corn peptide product.
Comparative example 1
The same as example 1, but without the second step treatment, the third step treatment was directly performed after the first step treatment.
Comparative example 2
The same as example 1, but the second step was changed to ultrasonic treatment for 30min and then the third step was carried out.
Comparative example 3
The same as example 1, but the second step was changed to microwave treatment for 30min and then the third step was carried out.
The results of the product index tests performed on the products obtained in examples 1 to 3 and on the corn peptide products obtained in comparative examples 1 to 3 are shown in Table I.
Watch 1
As can be seen from the data obtained in Table I, although the water contents of comparative examples 1-3 are similar to those of examples 1-3, the crude protein contents and peptide contents with molecular weights of 1000Da or less of comparative examples 1-3 are different from those of examples 1-3, so that the product prepared by the present invention has not only higher yield than the product prepared by the prior art, but also higher quality than the product prepared by the prior art.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (5)
1. A preparation method for preparing corn peptide by a heat treatment-assisted enzyme method is characterized by comprising the following steps: the method comprises the following steps:
the method comprises the following steps: pulverizing and refining the crude corn protein powder, adding distilled water into the pulverized corn protein powder, and fully and uniformly mixing to obtain a corn protein powder solution;
step two: heating the corn protein powder solution obtained in the step one to 90-120 ℃, and keeping the temperature for 20-40 min;
step three: after the heating in the second step is finished, cooling to 50-60 ℃, adding protease, and carrying out enzymolysis reaction for 4-8h under the stirring condition;
step four: and (3) drying the mixed solution subjected to the enzymolysis reaction in the third step at the temperature of 125-150 ℃ for 2-3h to obtain the corn peptide product.
2. The method for preparing corn peptide by heat treatment assisted enzyme method according to claim 1, wherein the method comprises the following steps: and in the step one, the crude corn protein powder is crushed and refined and then passes through a 40-mesh sieve for later use, and the particles with the particle size larger than 40-mesh sieve are continuously crushed until all the particles pass through the 40-mesh sieve.
3. The method for preparing corn peptide by heat treatment assisted enzyme method according to claim 1, wherein the method comprises the following steps: the corn protein powder solid content of the corn protein powder solution obtained in the step one is 8-20%.
4. The method for preparing corn peptide by heat treatment assisted enzyme method according to claim 1, wherein the method comprises the following steps: the protease in the third step is a mixture obtained by mixing any one or more than two of alkaline protease, neutral protease and flavourzyme in any proportion.
5. The method for preparing corn peptide by heat treatment assisted enzyme method according to claim 1, wherein the method comprises the following steps: the addition amount of the protease in the third step is 0.3-3.0% of the mass of the corn protein powder.
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| CN202010423050.6A CN111549086A (en) | 2020-05-19 | 2020-05-19 | Preparation method for preparing corn peptide by heat treatment-assisted enzyme method |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101531700A (en) * | 2008-03-12 | 2009-09-16 | 中国食品发酵工业研究院 | Maize oligopeptide and method for preparing same |
| CN109971811A (en) * | 2019-05-05 | 2019-07-05 | 新疆正生营养研究院（有限公司） | A kind of enzymatic isolation method prepares the method for corn functional peptides and the corn functional peptides of preparation |
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2020
- 2020-05-19 CN CN202010423050.6A patent/CN111549086A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101531700A (en) * | 2008-03-12 | 2009-09-16 | 中国食品发酵工业研究院 | Maize oligopeptide and method for preparing same |
| CN109971811A (en) * | 2019-05-05 | 2019-07-05 | 新疆正生营养研究院（有限公司） | A kind of enzymatic isolation method prepares the method for corn functional peptides and the corn functional peptides of preparation |
Non-Patent Citations (1)
| Title |
|---|
| JINWEI LIU等: "Optimization of Hydrolysis Conditions for Corn Gluten Meal", 《AGRICULTURAL SCIENCE & TECHNOLOGY》 * |
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Application publication date: 20200818 |