CN111543633A - Novel method for extracting okra seed polyphenol compound - Google Patents
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- CN111543633A CN111543633A CN202010421468.3A CN202010421468A CN111543633A CN 111543633 A CN111543633 A CN 111543633A CN 202010421468 A CN202010421468 A CN 202010421468A CN 111543633 A CN111543633 A CN 111543633A
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- 241001075517 Abelmoschus Species 0.000 title claims abstract description 20
- 235000013824 polyphenols Nutrition 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 19
- -1 polyphenol compound Chemical class 0.000 title abstract description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 14
- 238000000605 extraction Methods 0.000 claims abstract description 14
- 235000020238 sunflower seed Nutrition 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 108090000790 Enzymes Proteins 0.000 claims abstract description 12
- 102000004190 Enzymes Human genes 0.000 claims abstract description 12
- 229940088598 enzyme Drugs 0.000 claims abstract description 12
- 238000009210 therapy by ultrasound Methods 0.000 claims abstract description 10
- 108010059892 Cellulase Proteins 0.000 claims abstract description 9
- 108010059820 Polygalacturonase Proteins 0.000 claims abstract description 9
- 229940106157 cellulase Drugs 0.000 claims abstract description 9
- 108010093305 exopolygalacturonase Proteins 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 150000008442 polyphenolic compounds Chemical class 0.000 claims abstract description 9
- 238000004108 freeze drying Methods 0.000 claims abstract description 7
- 239000000843 powder Substances 0.000 claims abstract description 6
- 239000000284 extract Substances 0.000 claims abstract description 5
- 238000007873 sieving Methods 0.000 claims abstract description 4
- 239000006228 supernatant Substances 0.000 claims abstract description 4
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 230000009849 deactivation Effects 0.000 claims description 3
- 239000008176 lyophilized powder Substances 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 abstract description 6
- 210000002421 cell wall Anatomy 0.000 abstract description 4
- 239000002994 raw material Substances 0.000 abstract description 3
- 238000003287 bathing Methods 0.000 abstract 1
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- 239000003960 organic solvent Substances 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 12
- 235000016709 nutrition Nutrition 0.000 description 5
- 235000013305 food Nutrition 0.000 description 4
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 3
- CWEZAWNPTYBADX-UHFFFAOYSA-N Procyanidin Natural products OC1C(OC2C(O)C(Oc3c2c(O)cc(O)c3C4C(O)C(Oc5cc(O)cc(O)c45)c6ccc(O)c(O)c6)c7ccc(O)c(O)c7)c8c(O)cc(O)cc8OC1c9ccc(O)c(O)c9 CWEZAWNPTYBADX-UHFFFAOYSA-N 0.000 description 3
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- 235000003934 Abelmoschus esculentus Nutrition 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 235000009754 Vitis X bourquina Nutrition 0.000 description 2
- 235000012333 Vitis X labruscana Nutrition 0.000 description 2
- 235000014787 Vitis vinifera Nutrition 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
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- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000007760 free radical scavenging Effects 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
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- 238000012986 modification Methods 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 1
- PFTAWBLQPZVEMU-ZFWWWQNUSA-N (+)-epicatechin Natural products C1([C@@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-ZFWWWQNUSA-N 0.000 description 1
- PFTAWBLQPZVEMU-UKRRQHHQSA-N (-)-epicatechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-UKRRQHHQSA-N 0.000 description 1
- XFZJEEAOWLFHDH-UHFFFAOYSA-N (2R,2'R,3R,3'R,4R)-3,3',4',5,7-Pentahydroxyflavan(48)-3,3',4',5,7-pentahydroxyflavan Natural products C=12OC(C=3C=C(O)C(O)=CC=3)C(O)CC2=C(O)C=C(O)C=1C(C1=C(O)C=C(O)C=C1O1)C(O)C1C1=CC=C(O)C(O)=C1 XFZJEEAOWLFHDH-UHFFFAOYSA-N 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 229940123457 Free radical scavenger Drugs 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000208818 Helianthus Species 0.000 description 1
- 235000003222 Helianthus annuus Nutrition 0.000 description 1
- MOJZMWJRUKIQGL-FWCKPOPSSA-N Procyanidin C2 Natural products O[C@@H]1[C@@H](c2cc(O)c(O)cc2)Oc2c([C@H]3[C@H](O)[C@@H](c4cc(O)c(O)cc4)Oc4c3c(O)cc(O)c4)c(O)cc(O)c2[C@@H]1c1c(O)cc(O)c2c1O[C@@H]([C@H](O)C2)c1cc(O)c(O)cc1 MOJZMWJRUKIQGL-FWCKPOPSSA-N 0.000 description 1
- 241001295692 Pyracantha fortuneana Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 235000009392 Vitis Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 1
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 1
- 235000005487 catechin Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229950001002 cianidanol Drugs 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- LPTRNLNOHUVQMS-UHFFFAOYSA-N epicatechin Natural products Cc1cc(O)cc2OC(C(O)Cc12)c1ccc(O)c(O)c1 LPTRNLNOHUVQMS-UHFFFAOYSA-N 0.000 description 1
- 235000012734 epicatechin Nutrition 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000006408 oxalic acid Nutrition 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- HGVVOUNEGQIPMS-UHFFFAOYSA-N procyanidin Chemical compound O1C2=CC(O)=CC(O)=C2C(O)C(O)C1(C=1C=C(O)C(O)=CC=1)OC1CC2=C(O)C=C(O)C=C2OC1C1=CC=C(O)C(O)=C1 HGVVOUNEGQIPMS-UHFFFAOYSA-N 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- IKGXIBQEEMLURG-NVPNHPEKSA-N rutin Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-NVPNHPEKSA-N 0.000 description 1
- 238000002137 ultrasound extraction Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
- C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
- C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a method for extracting polyphenol compounds from sunflower seeds, which is characterized by comprising the following steps: (1) freeze-drying okra seeds, crushing, and sieving with a 60-mesh sieve; (2) taking okra seed freeze-dried powder, and adding 60% ethanol solution according to the material-liquid ratio of 1: 30; (3) adjusting pH to 3.5, adding cellulase and pectinase in the amount of 4.5mg/g cellulase and 40mg/g pectinase, water bathing at 50 deg.C for 30min, taking out, and inactivating enzyme; (4) treating with ultrasonic cell pulverizer at 210W for 30 min; (5) centrifuging the mixed system by using a centrifuge after ultrasonic treatment; (6) and (4) taking the centrifuged supernatant, and freeze-drying to obtain the okra seed polyphenol extract freeze-dried powder. The invention adopts the sunflower seeds with rich polyphenol compounds as raw materials, utilizes enzyme and ultrasonic waves to fully dissolve and crush the cell walls of the seeds, does not add organic solvent in the extraction process, successfully extracts the polyphenol in the sunflower seeds on the premise of safety and harmlessness, and obviously improves the extraction rate.
Description
Technical Field
The invention relates to a novel method for extracting okra seed polyphenol compounds, and belongs to the technical field of active substance extraction.
Background
Okra (Abelmoschus Medicus) is an annual, biennial or perennial herbaceous plant, mainly grown in tropical and subtropical regions of the eastern hemisphere, with the reputation of "the king of vegetables". The okra contains rich nutrient substances, vitamin C, dietary fiber and other substances, has extremely high calcium content and low oxalic acid content, has considerable absorption rate of calcium in a human body, and has been widely applied to the food industry along with the improvement of the yield of the okra and the continuous improvement and improvement of the processing technology of the okra in recent years. The seeds of the sunflower are rich in Procyanidins (PCs) and are biological active ingredients which are very important to human bodies. In the current food processing technology, the functions of the sunflower seeds are not paid more attention, and the rich nutritional value of the sunflower seeds is not widely utilized.
Procyanidine is called OPC for short, is a bioflavonoid with a special molecular structure, is prepared by condensing different amounts of catechin or epicatechin, has incomparable advantages in the aspect of antioxidation, is one of free radical scavengers with the best effect found at present, has good DPPH free radical scavenging capacity and ABTS free radical scavenging capacity, has a relatively obvious inhibiting effect on various pathogenic factors and viruses, and has the function of improving the color and taste of food. Procyanidin is widely present in fruits such as fructus Vitis Viniferae, and has strong antioxidant effect. In order to fully utilize the health-care function and the nutritional ingredients of polyphenol compounds represented by procyanidine in plants, some researchers extract the polyphenol compounds in different plants, such as grape seeds, black fungus and pyracantha fortuneana fruits, by adopting an enzymatic method and an ultrasonic extraction method. However, the existing procyanidine has few plant sources and low extraction rate of experiments, and generally adopted methods mainly comprise an enzyme-assisted solvent method, an ultrasonic microwave method and the like, for example, ultrasonic waves are adopted to extract procyanidine in grape seeds. The invention can also fully broaden the plant sources of the polyphenol compounds and fully exert the economic value and the nutritional value brought by the food processing field of the plant polyphenol.
Disclosure of Invention
The method takes the sunflower seeds which are rich in polyphenol compounds serving as main components and various functional nutritional components as raw materials, and performs full wall breaking treatment on the sunflower seeds by using enzyme and ultrasonic technology, so that the aim of extracting the polyphenol compounds which are mainly procyanidine in the sunflower seeds is fulfilled, and the characteristics of low extraction rate and difficult extraction of plant procyanidine are improved.
The method comprises the following steps: pretreatment of the sunflower seeds: freeze-drying okra seeds, fully crushing, sieving with a 60-mesh sieve, and bagging for later use;
step two: preparing feed liquid: taking a proper amount of okra seed freeze-dried powder, and adding the prepared ethanol solution according to the proportion, wherein the material-liquid ratio is 1:30, the concentration of ethanol is 60 percent;
step three: enzyme treatment: adjusting the pH value, adding pectinase and cellulase into the mixed system, placing the mixed system in a water bath kettle for water bath treatment, adjusting the pH value of the system to 3.5, adding the pectinase at the addition amount of 4.5mg/g, adding the cellulase at the addition amount of 40mg/g, controlling the water bath temperature at 50 ℃, controlling the water bath time to be 30min, and carrying out enzyme deactivation treatment after the reaction is finished;
step four: ultrasonic treatment: and (3) processing the mixed system by using an ultrasonic cell crushing instrument. The ultrasonic power of the ultrasonic cell crusher is 210W, and the ultrasonic treatment time is 30 min;
step five: centrifuging: centrifuging the mixed system by a centrifuge after ultrasonic treatment, wherein the centrifugal speed is 3000 Xg, and the centrifugal treatment time is 20 min;
step six: and taking the centrifuged supernatant, removing the bottom solid, and freeze-drying the supernatant to obtain the lyophilized powder of the sunflower seed polyphenol extract.
The invention has the beneficial effects that:
the method comprises using Abelmoschus esculentus seeds rich in polyphenols represented by procyanidine and various nutritional ingredients as raw materials, performing enzyme treatment, namely adding corresponding enzyme after the Abelmoschus esculentus seeds are freeze-dried and fully pulverized, and performing full treatment at proper temperature and pH. Both pectinase and cellulase can effectively enzymolyze plant cell walls, so that the plant cell walls are easy to extract; and then, the material liquid is processed by using an ultrasonic cell crushing instrument to further destroy the plant cell walls, thereby being beneficial to dissolving the procyanidine in the solvent. The extraction efficiency of the method is obviously higher than that of the procyanidine extraction process carried out by other plants and other methods. Therefore, the method can effectively improve the problem and the current situation that the extraction rate of the plant polyphenol is low to a certain extent, and fully exert the nutritional value of the functional polyphenol components.
Drawings
FIG. 1 is a process flow of a novel method for extracting polyphenol compounds from sunflower seeds
Detailed Description
The present invention is further described in detail below with reference to examples to enable those skilled in the art to practice the invention with reference to the description.
The experimental procedures described in the following embodiments are conventional unless otherwise specified. The reagents and materials are commercially available, unless otherwise specified.
The method comprises the following steps: pretreatment of the sunflower seeds: freeze-drying okra seeds, fully crushing, sieving with a 60-mesh sieve, and bagging for later use;
step two: preparing feed liquid: taking a proper amount of okra seed freeze-dried powder, and adding the prepared ethanol solution according to a ratio of 1:30, wherein the ethanol concentration is 60%;
step three: enzyme treatment: adjusting the pH value, adding pectinase and cellulase into the mixed system, placing the mixed system in a water bath kettle for water bath treatment, adjusting the pH value of the system to 3.5, adding the pectinase at the addition amount of 4.5mg/g, adding the cellulase at the addition amount of 40mg/g, controlling the water bath temperature at 50 ℃, controlling the water bath time to be 30min, and carrying out enzyme deactivation treatment after the reaction is finished;
step four: ultrasonic treatment: and (3) processing the mixed system by using an ultrasonic cell crushing instrument. The ultrasonic power of the ultrasonic cell crusher is 210W, and the ultrasonic treatment time is 30 min;
step five: centrifuging: centrifuging the mixed system by a centrifuge after ultrasonic treatment, wherein the centrifugal speed is 3000 Xg, and the centrifugal treatment time is 20 min;
while embodiments of the invention have been disclosed above, the foregoing is merely illustrative of the preferred practice of the invention. And are not intended to limit the invention in any other way. Any equivalent embodiment that may be changed or modified by the technical contents disclosed above to be equivalent to the above embodiment can be applied to other fields by those skilled in the art, but any simple modification to the above embodiment according to the technical essence of the present invention, which is equivalent to the change and modification, still belongs to the protection scope of the technical solution of the present invention without departing from the technical contents of the present invention.
Claims (5)
1. A novel okra seed polyphenol extraction process is characterized in that: the method comprises the following steps:
the method comprises the following steps: pretreatment of the sunflower seeds: selecting commercially available fresh okra, peeling seeds, freeze-drying the okra seeds, fully crushing, sieving with a 60-mesh sieve, and bagging for later use;
step two: preparing feed liquid: taking a proper amount of okra seed freeze-dried powder, and adding a prepared ethanol solution into the okra seed freeze-dried powder according to a certain proportion;
step three: enzyme treatment: adjusting the pH value to 3.5, adding pectinase and cellulase into the mixed system, putting the mixed system into a water bath kettle for water bath treatment, and performing enzyme deactivation treatment after the reaction is finished;
step four: ultrasonic treatment: processing the mixed system by using an ultrasonic cell crushing instrument;
step five: centrifuging: after ultrasonic treatment, a centrifugal machine is used for carrying out centrifugal treatment on the mixed system;
step six: the supernatant was taken and the bottom solid was discarded. Freeze drying the clear liquid to obtain the lyophilized powder of the polyphenol extract of the sunflower seeds.
2. The extraction process according to claim 1, characterized in that: in the second step, the ratio of the feed liquid to the feed liquid is 1:30, and the concentration of the ethanol is 60 percent.
3. The extraction process according to claim 1, characterized in that: in the third step, the pH value of the system is adjusted to be 3.5, the addition amount of the cellulase is 4.5mg/g, the addition amount of the pectinase is 40mg/g, the water bath temperature is 50 ℃, and the water bath time is 30 min.
4. The extraction process according to claim 1, characterized in that: in the fourth step, the ultrasonic power of the ultrasonic cell crusher is 210W, and the ultrasonic treatment time is 30 min.
5. The extraction process according to claim 1, characterized in that: in the fifth step, the centrifugal rotating speed is 3000 Xg, and the centrifugal processing time is 20 min.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102040577A (en) * | 2010-09-27 | 2011-05-04 | 南京泽朗医药科技有限公司 | Method for purifying flavone of okra |
| CN105440000A (en) * | 2015-11-13 | 2016-03-30 | 刘亚平 | Extraction process of hibiscus esculentus flavone |
| CN106924305A (en) * | 2017-05-05 | 2017-07-07 | 龙门县绿天源生态农业发展有限公司 | A kind of gumbo extract and its preparation method and application |
-
2020
- 2020-05-18 CN CN202010421468.3A patent/CN111543633A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102040577A (en) * | 2010-09-27 | 2011-05-04 | 南京泽朗医药科技有限公司 | Method for purifying flavone of okra |
| CN105440000A (en) * | 2015-11-13 | 2016-03-30 | 刘亚平 | Extraction process of hibiscus esculentus flavone |
| CN106924305A (en) * | 2017-05-05 | 2017-07-07 | 龙门县绿天源生态农业发展有限公司 | A kind of gumbo extract and its preparation method and application |
Non-Patent Citations (2)
| Title |
|---|
| 慢生活工坊: "《绿色蔬菜栽培入门手册》", 30 November 2018, 福建科学技术出版社 * |
| 梁彬霞，等: "黄秋葵籽的功能性成分及综合开发利用的研究进展", 《农产品加工》 * |
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