CN111543360A - Method for measuring deposition rate of calcium carbonate for culturing shellfish and application - Google Patents
Method for measuring deposition rate of calcium carbonate for culturing shellfish and application Download PDFInfo
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- CN111543360A CN111543360A CN202010431782.XA CN202010431782A CN111543360A CN 111543360 A CN111543360 A CN 111543360A CN 202010431782 A CN202010431782 A CN 202010431782A CN 111543360 A CN111543360 A CN 111543360A
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- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 title claims abstract description 99
- 235000015170 shellfish Nutrition 0.000 title claims abstract description 72
- 238000000034 method Methods 0.000 title claims abstract description 53
- 229910000019 calcium carbonate Inorganic materials 0.000 title claims abstract description 49
- 230000008021 deposition Effects 0.000 title claims abstract description 48
- 238000012258 culturing Methods 0.000 title claims description 8
- 239000013535 sea water Substances 0.000 claims abstract description 68
- 238000005259 measurement Methods 0.000 claims abstract description 16
- 238000012544 monitoring process Methods 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 28
- 238000005070 sampling Methods 0.000 claims description 20
- 229920003023 plastic Polymers 0.000 claims description 12
- 239000004033 plastic Substances 0.000 claims description 12
- 241000237502 Ostreidae Species 0.000 claims description 10
- 235000020636 oyster Nutrition 0.000 claims description 10
- 238000005086 pumping Methods 0.000 claims description 10
- 241000237536 Mytilus edulis Species 0.000 claims description 8
- 235000020638 mussel Nutrition 0.000 claims description 8
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical class Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 claims description 7
- 235000020637 scallop Nutrition 0.000 claims description 7
- 241000237891 Haliotidae Species 0.000 claims description 4
- 241000237503 Pectinidae Species 0.000 claims description 4
- 230000007797 corrosion Effects 0.000 claims description 4
- 238000005260 corrosion Methods 0.000 claims description 4
- 241000237509 Patinopecten sp. Species 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- 241000237858 Gastropoda Species 0.000 claims description 2
- RCTYPNKXASFOBE-UHFFFAOYSA-M chloromercury Chemical class [Hg]Cl RCTYPNKXASFOBE-UHFFFAOYSA-M 0.000 claims 1
- 238000004062 sedimentation Methods 0.000 claims 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 5
- 230000008901 benefit Effects 0.000 description 3
- 230000004071 biological effect Effects 0.000 description 3
- 230000007547 defect Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 2
- 240000000249 Morus alba Species 0.000 description 1
- 235000008708 Morus alba Nutrition 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- JYYOBHFYCIDXHH-UHFFFAOYSA-N carbonic acid;hydrate Chemical compound O.OC(O)=O JYYOBHFYCIDXHH-UHFFFAOYSA-N 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 229960002523 mercuric chloride Drugs 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004584 weight gain Effects 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
- 238000004804 winding Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/51—Culture of aquatic animals of shellfish of gastropods, e.g. abalones or turban snails
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention belongs to the technical field of seawater shellfish culture, and discloses a method for measuring the deposition rate of cultured shellfish calcium carbonate and application thereof. The invention reflects the growth condition of the shellfish based on the precise component method of analytical chemistry, the whole operation can be completed within 1 day, the intervention of the field measurement in the culture area on the shellfish growth can be reduced to the minimum, the growth of the tested individual at the next stage is not influenced, and the invention is suitable for monitoring the instant growth condition of the shellfish. Compared with the prior art, the method provides direct and instant data reference for the farmers to master the growth conditions of the cultured shellfish in real time, and provides powerful support for the farmers to more flexibly make and improve culture schemes.
Description
Technical Field
The invention belongs to the technical field of seawater shellfish culture, and particularly relates to a method for measuring the deposition rate of cultured shellfish calcium carbonate and application thereof.
Background
At present, the traditional method for monitoring the growth of the shellfish is to measure the length and the width of the shell by using a ruler and weigh the shellfish by using balance. Since the growth and weight gain of the shells take a long time, it is necessary to wait for 20 to 30 days to grasp the growth condition of the shellfish. The traditional method is time-consuming, and shellfish individuals growing and winding together need to be separated in the measuring process, so that the time and labor are wasted, the shells are damaged, and the growth of the measured individuals is influenced. The calcium carbonate deposition rate is a direct, reliable and accurate index of shellfish shell growth, and although the index can be measured in an indoor controllable environment, the outdoor environment is complex, and no mature field measurement method can be applied at present.
Through the above analysis, the problems and defects of the prior art are as follows: the traditional method for monitoring the shellfish growth is long in time consumption, wastes time and labor, can damage shells and influences the growth of measured individuals.
The difficulty in solving the above problems and defects is: how to obtain the growth condition of the cultured shellfish in a short time and labor-saving way, and the shellfish shell is not damaged in the operation process and the growth of the next stage is not interfered. Meanwhile, the operation flow is needed to be realized quickly, easily and effortlessly.
The significance of solving the problems and the defects is as follows: compared with the prior art, the method provides direct and instant data reference for the farmers to master the growth conditions of the cultured shellfish in real time, does not damage the shells of the shellfish and influence the growth of the shells, and provides powerful support for the farmers to flexibly make and improve the culture scheme.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a method for measuring the deposition rate of calcium carbonate for cultivating shellfish and application thereof.
The method for measuring the deposition rate of the calcium carbonate for the cultured shellfish comprises the steps of weighing shellfish to be measured on site, temporarily culturing running water for a short time, measuring the running water rate, sampling and storing the cultured seawater on site, measuring the total alkalinity of a seawater sample indoors, and calculating the deposition rate of the calcium carbonate for the cultured shellfish to be measured according to the measured weight and the running water rate of the shellfish;
the method for measuring the deposition rate of the calcium carbonate for the cultured shellfish adopts a total alkalinity difference method and adopts the following formula to calculate the deposition rate of the calcium carbonate:
wherein G: calcium carbonate deposition rate in units of μmolg-1h-1；TAi、TAf: the total alkalinity of the seawater and the pumped seawater on site is in mu molL-1(ii) a V: the water pump pumps water at a rate of lh-1(ii) a M: the total weight of the experimental shellfish is g.
Furthermore, the cultured shellfish of the method for measuring the deposition rate of the calcium carbonate of the cultured shellfish comprises oysters, mussels, scallops, bivalves, abalones and snails.
Furthermore, the method for measuring the deposition rate of the calcium carbonate for culturing the shellfish needs one whole string or cage of shellfish to be measured.
Further, the method for measuring the deposition rate of calcium carbonate in the cultured shellfish comprises the following steps:
(1) measuring fresh weight of a whole string of suspended oysters or mussels and scallops and abalones cultured in a whole cage, putting the measured fresh weight into a plastic cylinder with two open ends, immersing the cylinder into seawater vertically, and fixing the top end of the cylinder to a ship board of a fishing boat, wherein the top end of the cylinder is slightly higher than the sea level;
(2) immersing the submersible pump into the seawater at the top end of the cylinder;
(3) the submersible pump is connected with a power supply, seawater is pumped out from the top end of the cylinder, the seawater being pumped out is connected into a bucket with the volume accurately calibrated, timing is respectively carried out from the beginning to the end of pumping, and the flowing water speed V is calculated according to the accurately calibrated volume and the pumping time;
(4) after the flow rate measurement is finished, sampling the seawater pumped out into a sample bottle; simultaneously sampling on-site seawater and storing the seawater into a sample bottle; saturated mercuric chloride was added to all the sampling bottles.
Further, oyster or mussel that (1) will whole string of hanging foster, scallop, the abalone of whole cage breed, measure the fresh weight after, put into length 2m, diameter 30 ~ 50cm, in the equal open-ended plastic cylinder in both ends, cylinder thickness 2 ~ 3 mm.
Further, the submersible pump with the lift of 2-4 m, the flow speed of 2-4 m, multiple adjustable gears and seawater corrosion resistance is selected in the step (2).
Further, the power supply voltage of (3) is 220v, and the storage capacity is more than 500 Wh; the submersible pump is connected with a power supply, seawater is pumped out from the top end of the cylinder, and after 5 minutes, the seawater being pumped out is connected into a water bucket with the volume accurately calibrated to 10L-15L.
Further, after the flow rate measurement (4) is finished, the seawater being pumped out is sampled to a sample bottle with a volume of 200 ml.
Further, 0.1ml of saturated mercuric chloride was added to all the sampling bottles of the above (4).
The cylinder of this specification just in time can hold whole breed cage, convenient operation. The adaptive water pump is easy to find out by the storage battery with voltage and electric quantity, multiple field measurements can be carried out, the times of repeated measurement are increased, and the accuracy is improved. The water pump with the lift of 2-4 m can ensure the smooth operation of the experiment. The pump lift is small, the water pumping speed is low, the experiment consumes long time, and the pump lift is large, so that the electric quantity of the storage battery can be consumed too fast, and the number of times of field measurement is reduced. The sampling seawater 200ml is added with 0.1ml of mercuric chloride, which can ensure that all biological activities are inhibited, thereby preventing the influence of microorganisms and the like on a water carbonate system. Because the method of the present invention is based on accurate carbonate system measurements, it is desirable to minimize the impact of other organisms on the carbonate system.
Another object of the present invention is to provide a method for monitoring shellfish growth using the method for determining the deposition rate of calcium carbonate in cultured shellfish.
By combining all the technical schemes, the invention has the advantages and positive effects that: the method is based on the seawater chemical principle, can master the deposition rate of the shellfish calcium carbonate in one day by measuring the change of a seawater carbonate system and combining the field experiment in a culture area, and reflects the growth condition of the shellfish to be detected. The method is based on the analysis and determination method of seawater chemistry, so that the obtained data is more accurate and reliable. And relative to a conventional 20-30 day measurement period. The invention can obtain accurate results on the same day, and simply, conveniently and quickly monitor the instant growth condition of the cultured shellfish. A rapid and accurate determination method of the calcium carbonate deposition rate of the marine cultured shellfish. The method has the advantage of reflecting the actual culture condition in real time and accurately.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments of the present application will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained from the drawings without creative efforts.
FIG. 1 is a flow chart of a method for measuring the deposition rate of calcium carbonate in cultured shellfish according to the embodiment of the invention.
FIG. 2 is a schematic structural diagram of a device for measuring the deposition rate of calcium carbonate in cultured shellfish according to the embodiment of the invention;
in the figure: 1. a plastic cylinder; 2. a submersible pump; 3. hanging a rope; 4. a conduit; 5. a water bucket.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides a method for measuring the deposition rate of calcium carbonate for cultivating shellfish and an application thereof, and the invention is described in detail with reference to the accompanying drawings.
As shown in figure 1, the method for measuring the deposition rate of the calcium carbonate for cultivating shellfish provided by the invention comprises the following steps:
s101: weighing shellfish to be detected on site, temporarily culturing in flowing water for a short time, and measuring the flowing water rate;
s102: sampling and storing the cultivated seawater on site;
s103: indoor measurement of total alkalinity of seawater samples;
s104: and calculating the deposition rate of the calcium carbonate of the cultured shellfish to be detected according to the measured shellfish weight, the water flowing speed and the like.
The method for determining the deposition rate of the calcium carbonate for cultivating the shellfish comprises the following steps:
(1) oyster or mussel of will whole cluster of hanging foster, scallop, the abalone of whole cage breed, measure fresh and heavy back, put into length 2m, diameter 30 ~ 50cm, in the equal open-ended plastics drum in both ends, drum thickness 2 ~ 3mm, drum dip seawater perpendicularly, the top is a little higher than the sea level, the drum top is fixed to the fishing boat ship side.
(2) The method comprises the steps of selecting a submersible pump with the lift of 2-4 m, the flow speed of multiple gears of adjustable speed and seawater corrosion resistance, immersing the submersible pump into seawater at the top end of a cylinder, and ensuring that the submersible pump is slightly higher than an individual to be tested.
(3) The fully charged portable power supply is selected, the voltage is 220v, and the storage capacity is more than 500 Wh. The submersible pump is connected with a power supply, seawater is pumped out from the top end of the cylinder, after 5 minutes, the seawater being pumped out is connected into a bucket with the volume accurately calibrated to 10L-15L, the time is respectively timed from the beginning to the end of pumping, and the flowing speed V (lh) is calculated through the accurately calibrated volume and the pumping time-1)。
(4) After the flow rate measurement is finished, the seawater being pumped out is sampled to a sample bottle with the volume of 200 ml. And simultaneously sampling on-site seawater and storing the seawater into a sample bottle. Saturated mercuric chloride 0.1ml is added into all sampling bottles to prevent the influence of biological activities on a seawater sample carbonate system.
(5) Measuring the total alkalinity of a seawater sample to be measured by adopting an automatic total alkalinity titrator, and calculating the deposition rate of calcium carbonate by applying the following formula according to a total alkalinity difference method:
g: calcium carbonate deposition Rate (. mu.molg)-1h-1)；
TAi、TAf: total alkalinity (mu molL) of on-site seawater and pumped-out seawater-1)；
V: speed of water pump (lh)-1)；
M: total weight of the shellfish (g) was tested.
As shown in fig. 2, the device for measuring the deposition rate of calcium carbonate for cultivating shellfish provided by the embodiment of the invention comprises: plastic cylinder 1, immersible pump 2, string 3, pipe 4 and cask 5.
The upper end of the plastic cylinder 1 is welded with a hanging ring, and a hanging rope 3 is bound by the hanging ring; a submersible pump 2 is arranged in the plastic cylinder 1, and the submersible pump 2 is connected with a water barrel 5 through a conduit 4. Wherein the plastic cylinder 1 is open at the top and bottom.
The whole string of suspended oysters or mussels is measured for freshness and weight and then put into a plastic cylinder 1 with openings at two ends, the cylinder is vertically immersed in seawater, the top end of the cylinder is slightly higher than the sea level, and the top end of the cylinder is fixed on the side of a fishing boat; immersing the submersible pump 2 in the seawater at the top end of the cylinder; the submersible pump 2 is connected with a power supply, pumps out seawater from the top end of the cylinder, connects the seawater being pumped into the water barrel 5 with accurate volume calibration, respectively times from the beginning to the end of pumping, and calculates the flowing water speed V according to the accurately calibrated volume and the pumping time; after the flow rate measurement is finished, sampling the seawater pumped out into a sample bottle; simultaneously sampling on-site seawater and storing the seawater into a sample bottle; saturated mercuric chloride was added to all the sampling bottles.
The technical solution of the present invention is further described with reference to the following specific examples.
The method for determining the deposition rate of the calcium carbonate for cultivating the shellfish comprises the following steps:
(1) in 7 months, 3 strings of suspended oysters are weighed to 1560g, 1786g and 1456g in the area of the Rongcheng Mulberry gulf sea, and put into a plastic cylinder with the length of 2m, the diameter of 30cm and two open ends, the thickness of the cylinder is 2-3 mm, the cylinder is immersed into the cylinder and vertically immersed into seawater, the top end of the cylinder is slightly higher than the sea level, and the top end of the cylinder is fixed on the side of a fishing boat.
(2) Adopt 2 ~ 4m of lift, many grades of adjustable and resistant sea water corrosion's of velocity of flow immerse the immersible pump (yihan, new exquisite 1000) in the sea water on drum top, guarantee that the immersible pump is a little higher than the individual that awaits measuring.
(3) A fully charged portable mobile power supply (DXPOWER/Electricity Xiao' II; 1000W outdoor power supply) is selected, the voltage is 220v, and the storage capacity is 1002 Wh. The submersible pump is connected with a power supply, seawater is pumped out from the top end of the cylinder, after 5 minutes, the seawater being pumped out is connected into a bucket with the volume accurately calibrated to 15L, the water pumping time is 96s, and therefore the water flow speed V is 562.5L/h.
(4) After the flow rate measurement is finished, the seawater being pumped out is sampled to a sample bottle with the volume of 200 ml. And simultaneously sampling on-site seawater and storing the seawater into a sample bottle. Saturated mercuric chloride 0.1ml is added into all sampling bottles to prevent the influence of biological activities on a seawater sample carbonate system.
(5) The total alkalinity TA was determined immediately after sampling and was measured by spot-location method using a 848Titrino plus alkalinity autotitrator manufactured by the company Vantohm, Switzerland (Metrohm). The total alkalinity of the on-site seawater is 2200 [ mu ] moll-1The total alkalinity of the pumped seawater is 2148 mu moll respectively-1、2133μmoll-1And 2160. mu. moll-1。
(6) And (3) adopting a total alkalinity difference method, substituting the measured numerical value into the following formula to calculate the deposition rate of the calcium carbonate. The calculated calcium carbonate deposition rate of the oyster shell of the hanging sample in the gulf of Morus alba is 9.21 +/-1.15 mu molg-1h-1. Indicating that the calcium carbonate deposition rate of oysters in this region during 7 months was at a higher level.
Calculation of calcium carbonate deposition rate:
g: calcium carbonate deposition Rate (. mu.molg)-1h-1)；
TAi、TAf: total alkalinity (mu molL) of on-site seawater and pumped-out seawater-1)；
V: water pumpRate of (lh)-1)；
M: total weight of the shellfish (g) was tested.
In the description of the present invention, "a plurality" means two or more unless otherwise specified; the terms "upper", "lower", "left", "right", "inner", "outer", "front", "rear", "head", "tail", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are only for convenience in describing and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, should not be construed as limiting the invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
The above description is only for the purpose of illustrating the present invention and the appended claims are not to be construed as limiting the scope of the invention, which is intended to cover all modifications, equivalents and improvements that are within the spirit and scope of the invention as defined by the appended claims.
Claims (10)
1. A method for determining the deposition rate of calcium carbonate for culturing shellfish is characterized in that the method for determining the deposition rate of calcium carbonate for culturing shellfish comprises the steps of on-site weighing of shellfish to be determined, temporary running water culture, determination of running water rate, on-site sampling and storage of seawater after culture, indoor measurement of total alkalinity of a seawater sample, and calculation of the deposition rate of calcium carbonate for culturing shellfish to be determined according to the measured weight and running water rate of the shellfish;
the method for measuring the deposition rate of the calcium carbonate for the cultured shellfish adopts a total alkalinity difference method and adopts the following formula to calculate the deposition rate of the calcium carbonate:
wherein G: calcium carbonate deposition rateIn units of μmolg-1h-1；TAi、TAf: the total alkalinity of the seawater and the pumped seawater on site is in mu molL-1(ii) a V: the water pump pumps water at a rate of lh-1(ii) a M: the total weight of the experimental shellfish is g.
2. The method for measuring the deposition rate of calcium carbonate in cultured shellfish according to claim 1, wherein the cultured shellfish used in the method for measuring the deposition rate of calcium carbonate in cultured shellfish comprises bivalves such as oyster, mussel and scallop, abalone and snail.
3. The method for measuring the deposition rate of calcium carbonate for cultivating shellfish according to claim 1, wherein the number of shellfish to be measured in one measurement is a whole string or a whole cage.
4. The method for measuring the deposition rate of calcium carbonate in cultured shellfish according to claim 1, wherein the method for measuring the deposition rate of calcium carbonate in cultured shellfish comprises the steps of:
(1) measuring fresh weight of a whole string of suspended oysters or mussels and scallops and abalones cultured in a whole cage, putting the measured fresh weight into a plastic cylinder with two open ends, immersing the cylinder into seawater vertically, and fixing the top end of the cylinder to a ship board of a fishing boat, wherein the top end of the cylinder is slightly higher than the sea level;
(2) immersing the submersible pump into the seawater at the top end of the cylinder;
(3) the submersible pump is connected with a power supply, seawater is pumped out from the top end of the cylinder, the seawater being pumped out is connected into a bucket with the volume accurately calibrated, timing is respectively carried out from the beginning to the end of pumping, and the flowing water speed V is calculated according to the accurately calibrated volume and the pumping time;
(4) after the flow rate measurement is finished, sampling the seawater pumped out into a sample bottle; simultaneously sampling on-site seawater and storing the seawater into a sample bottle; saturated mercuric chloride was added to all the sampling bottles.
5. The method for measuring the deposition rate of calcium carbonate for cultivating shellfish according to claim 1, wherein (1) the whole string of hung oysters or mussels, the whole cage of cultivated scallops or abalones is measured for fresh weight and then placed into a plastic cylinder with the length of 2m, the diameter of 30-50 cm and two open ends, and the thickness of the cylinder is 2-3 mm.
6. The method for determining the deposition rate of the calcium carbonate for the cultured shellfish according to claim 1, wherein the submersible pump (2) with the lift of 2-4 m, the flow rate of 2-4 m, multiple adjustable gears and seawater corrosion resistance is selected.
7. The method for measuring the deposition rate of calcium carbonate in cultured shellfish according to claim 1, wherein (3) the power supply voltage is 220v, the storage capacity is more than 500 Wh; the submersible pump is connected with a power supply, seawater is pumped out from the top end of the cylinder, and after 5 minutes, the seawater being pumped out is connected into a water bucket with the volume accurately calibrated to 10L-15L.
8. The method for measuring the sedimentation rate of calcium carbonate in cultured shellfish according to claim 1, wherein, after the flow rate measurement in (4) is finished, the seawater being pumped is sampled to a sample bottle with a volume of 200 ml.
9. The method for measuring the calcium carbonate deposition rate of the cultured shellfish according to claim 1, wherein 0.1ml of saturated mercury chloride is added into all the sampling bottles in the step (4).
10. A method for monitoring shellfish growth, characterized in that the method for monitoring shellfish growth uses the method for determining the deposition rate of calcium carbonate in cultured shellfish as claimed in any of claims 1-9.
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