CN111529557B - Application of plant fruit oil in preparation of medicines and health foods for preventing ovarian injury caused by radiotherapy and chemotherapy - Google Patents
Application of plant fruit oil in preparation of medicines and health foods for preventing ovarian injury caused by radiotherapy and chemotherapy Download PDFInfo
- Publication number
- CN111529557B CN111529557B CN202010525333.1A CN202010525333A CN111529557B CN 111529557 B CN111529557 B CN 111529557B CN 202010525333 A CN202010525333 A CN 202010525333A CN 111529557 B CN111529557 B CN 111529557B
- Authority
- CN
- China
- Prior art keywords
- chemotherapy
- radiotherapy
- ovarian
- seed oil
- application
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000002611 ovarian Effects 0.000 title claims abstract description 50
- 238000002512 chemotherapy Methods 0.000 title claims abstract description 48
- 230000006378 damage Effects 0.000 title claims abstract description 44
- 238000001959 radiotherapy Methods 0.000 title claims abstract description 43
- 208000027418 Wounds and injury Diseases 0.000 title claims abstract description 25
- 208000014674 injury Diseases 0.000 title claims abstract description 25
- 239000003814 drug Substances 0.000 title claims abstract description 24
- 235000013402 health food Nutrition 0.000 title claims description 7
- 235000013399 edible fruits Nutrition 0.000 title abstract description 17
- 229940079593 drug Drugs 0.000 title abstract description 15
- 238000002360 preparation method Methods 0.000 title abstract description 3
- 241000229143 Hippophae Species 0.000 claims abstract description 59
- 235000003935 Hippophae Nutrition 0.000 claims description 6
- 239000000839 emulsion Substances 0.000 claims description 6
- 239000000865 liniment Substances 0.000 claims description 6
- 239000006210 lotion Substances 0.000 claims description 6
- 239000002674 ointment Substances 0.000 claims description 6
- 239000006187 pill Substances 0.000 claims description 6
- 239000007901 soft capsule Substances 0.000 claims description 6
- 239000000829 suppository Substances 0.000 claims description 6
- 239000003826 tablet Substances 0.000 claims description 6
- 229940040145 liniment Drugs 0.000 claims 2
- 235000003145 Hippophae rhamnoides Nutrition 0.000 abstract description 53
- 235000015112 vegetable and seed oil Nutrition 0.000 abstract description 46
- 210000001672 ovary Anatomy 0.000 abstract description 36
- 230000012173 estrus Effects 0.000 abstract description 27
- 235000019198 oils Nutrition 0.000 abstract description 16
- 241000196324 Embryophyta Species 0.000 abstract description 14
- 230000000694 effects Effects 0.000 abstract description 11
- 230000002265 prevention Effects 0.000 abstract description 9
- 235000013305 food Nutrition 0.000 abstract description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 5
- 241000699670 Mus sp. Species 0.000 description 22
- 230000006870 function Effects 0.000 description 19
- 108010005853 Anti-Mullerian Hormone Proteins 0.000 description 18
- 102100030173 Muellerian-inhibiting factor Human genes 0.000 description 18
- 239000000868 anti-mullerian hormone Substances 0.000 description 17
- 241000699666 Mus <mouse, genus> Species 0.000 description 14
- 210000004027 cell Anatomy 0.000 description 14
- 210000001519 tissue Anatomy 0.000 description 12
- 206010028980 Neoplasm Diseases 0.000 description 9
- 239000002246 antineoplastic agent Substances 0.000 description 8
- 230000003325 follicular Effects 0.000 description 8
- 239000013641 positive control Substances 0.000 description 8
- COVZYZSDYWQREU-UHFFFAOYSA-N Busulfan Chemical compound CS(=O)(=O)OCCCCOS(C)(=O)=O COVZYZSDYWQREU-UHFFFAOYSA-N 0.000 description 7
- CMSMOCZEIVJLDB-UHFFFAOYSA-N Cyclophosphamide Chemical compound ClCCN(CCCl)P1(=O)NCCCO1 CMSMOCZEIVJLDB-UHFFFAOYSA-N 0.000 description 7
- 229960002092 busulfan Drugs 0.000 description 7
- 229960004397 cyclophosphamide Drugs 0.000 description 7
- 229940044683 chemotherapy drug Drugs 0.000 description 6
- 210000002503 granulosa cell Anatomy 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 230000008859 change Effects 0.000 description 5
- 229940011871 estrogen Drugs 0.000 description 5
- 239000000262 estrogen Substances 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 108010050144 Triptorelin Pamoate Proteins 0.000 description 4
- 210000004246 corpus luteum Anatomy 0.000 description 4
- 230000007423 decrease Effects 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 239000002552 dosage form Substances 0.000 description 4
- 210000002919 epithelial cell Anatomy 0.000 description 4
- 210000000265 leukocyte Anatomy 0.000 description 4
- 230000016087 ovulation Effects 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- 238000010186 staining Methods 0.000 description 4
- 208000024891 symptom Diseases 0.000 description 4
- 238000011282 treatment Methods 0.000 description 4
- VXKHXGOKWPXYNA-PGBVPBMZSA-N triptorelin Chemical compound C([C@@H](C(=O)N[C@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N1[C@@H](CCC1)C(=O)NCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)[C@H]1NC(=O)CC1)C1=CC=C(O)C=C1 VXKHXGOKWPXYNA-PGBVPBMZSA-N 0.000 description 4
- 229960004824 triptorelin Drugs 0.000 description 4
- 238000010171 animal model Methods 0.000 description 3
- 208000035475 disorder Diseases 0.000 description 3
- 230000003203 everyday effect Effects 0.000 description 3
- 229940088597 hormone Drugs 0.000 description 3
- 239000005556 hormone Substances 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 230000028327 secretion Effects 0.000 description 3
- 201000000736 Amenorrhea Diseases 0.000 description 2
- 206010001928 Amenorrhoea Diseases 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 206010016654 Fibrosis Diseases 0.000 description 2
- 102000006771 Gonadotropins Human genes 0.000 description 2
- 108010086677 Gonadotropins Proteins 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- 208000019255 Menstrual disease Diseases 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 210000000683 abdominal cavity Anatomy 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 231100000540 amenorrhea Toxicity 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 229940041181 antineoplastic drug Drugs 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 210000003756 cervix mucus Anatomy 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 230000035558 fertility Effects 0.000 description 2
- 230000004761 fibrosis Effects 0.000 description 2
- 239000002622 gonadotropin Substances 0.000 description 2
- 230000002055 immunohistochemical effect Effects 0.000 description 2
- 238000003364 immunohistochemistry Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000005906 menstruation Effects 0.000 description 2
- 238000000465 moulding Methods 0.000 description 2
- 238000010172 mouse model Methods 0.000 description 2
- 229940127234 oral contraceptive Drugs 0.000 description 2
- 239000003539 oral contraceptive agent Substances 0.000 description 2
- 201000004535 ovarian dysfunction Diseases 0.000 description 2
- 231100000377 ovarian toxicity Toxicity 0.000 description 2
- 230000027758 ovulation cycle Effects 0.000 description 2
- 210000004681 ovum Anatomy 0.000 description 2
- 239000012188 paraffin wax Substances 0.000 description 2
- 230000010412 perfusion Effects 0.000 description 2
- 230000003449 preventive effect Effects 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 238000011127 radiochemotherapy Methods 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 210000004291 uterus Anatomy 0.000 description 2
- 210000001215 vagina Anatomy 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- GVJHHUAWPYXKBD-IEOSBIPESA-N α-tocopherol Chemical compound OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-IEOSBIPESA-N 0.000 description 2
- HBOQXIRUPVQLKX-UHFFFAOYSA-N 2,3-di(octadeca-9,12-dienoyloxy)propyl octadeca-9,12-dienoate Chemical compound CCCCCC=CCC=CCCCCCCCC(=O)OCC(OC(=O)CCCCCCCC=CCC=CCCCCC)COC(=O)CCCCCCCC=CCC=CCCCCC HBOQXIRUPVQLKX-UHFFFAOYSA-N 0.000 description 1
- 206010002383 Angina Pectoris Diseases 0.000 description 1
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- 206010003694 Atrophy Diseases 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 206010065559 Cerebral arteriosclerosis Diseases 0.000 description 1
- 206010008342 Cervix carcinoma Diseases 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 208000030453 Drug-Related Side Effects and Adverse reaction Diseases 0.000 description 1
- NMJREATYWWNIKX-UHFFFAOYSA-N GnRH Chemical compound C1CCC(C(=O)NCC(N)=O)N1C(=O)C(CC(C)C)NC(=O)C(CC=1C2=CC=CC=C2NC=1)NC(=O)CNC(=O)C(NC(=O)C(CO)NC(=O)C(CC=1C2=CC=CC=C2NC=1)NC(=O)C(CC=1NC=NC=1)NC(=O)C1NC(=O)CC1)CC1=CC=C(O)C=C1 NMJREATYWWNIKX-UHFFFAOYSA-N 0.000 description 1
- 208000033830 Hot Flashes Diseases 0.000 description 1
- 206010060800 Hot flush Diseases 0.000 description 1
- 208000031226 Hyperlipidaemia Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 description 1
- 101001011651 Mus musculus Muellerian-inhibiting factor Proteins 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 208000001132 Osteoporosis Diseases 0.000 description 1
- 206010033128 Ovarian cancer Diseases 0.000 description 1
- 206010033165 Ovarian failure Diseases 0.000 description 1
- 206010061535 Ovarian neoplasm Diseases 0.000 description 1
- 102000004451 Pituitary Gonadotropins Human genes 0.000 description 1
- 108010081865 Pituitary Gonadotropins Proteins 0.000 description 1
- 206010036590 Premature baby Diseases 0.000 description 1
- 101000857870 Squalus acanthias Gonadoliberin Proteins 0.000 description 1
- 208000005718 Stomach Neoplasms Diseases 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 229940087168 alpha tocopherol Drugs 0.000 description 1
- JKOQGQFVAUAYPM-UHFFFAOYSA-N amifostine Chemical compound NCCCNCCSP(O)(O)=O JKOQGQFVAUAYPM-UHFFFAOYSA-N 0.000 description 1
- 229960001097 amifostine Drugs 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 230000031016 anaphase Effects 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 230000037444 atrophy Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 235000005473 carotenes Nutrition 0.000 description 1
- 150000001746 carotenes Chemical class 0.000 description 1
- 201000010881 cervical cancer Diseases 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 230000001054 cortical effect Effects 0.000 description 1
- 229940127089 cytotoxic agent Drugs 0.000 description 1
- 238000013480 data collection Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 238000011038 discontinuous diafiltration by volume reduction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 230000035611 feeding Effects 0.000 description 1
- 210000004996 female reproductive system Anatomy 0.000 description 1
- 210000005002 female reproductive tract Anatomy 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 206010017758 gastric cancer Diseases 0.000 description 1
- 210000004392 genitalia Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000007490 hematoxylin and eosin (H&E) staining Methods 0.000 description 1
- 230000003054 hormonal effect Effects 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
- 230000002267 hypothalamic effect Effects 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 201000005851 intracranial arteriosclerosis Diseases 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 238000011866 long-term treatment Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 230000004630 mental health Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000004660 morphological change Effects 0.000 description 1
- 208000013435 necrotic lesion Diseases 0.000 description 1
- 230000001613 neoplastic effect Effects 0.000 description 1
- 206010029410 night sweats Diseases 0.000 description 1
- 230000036565 night sweats Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000000287 oocyte Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 208000015124 ovarian disease Diseases 0.000 description 1
- 231100000543 ovarian dysfunction Toxicity 0.000 description 1
- 231100000539 ovarian failure Toxicity 0.000 description 1
- 210000002394 ovarian follicle Anatomy 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 230000031877 prophase Effects 0.000 description 1
- 239000011251 protective drug Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000002271 resection Methods 0.000 description 1
- 230000035946 sexual desire Effects 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 210000004085 squamous epithelial cell Anatomy 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 201000011549 stomach cancer Diseases 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- 230000000451 tissue damage Effects 0.000 description 1
- 231100000827 tissue damage Toxicity 0.000 description 1
- 229960000984 tocofersolan Drugs 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- NCYCYZXNIZJOKI-UHFFFAOYSA-N vitamin A aldehyde Natural products O=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-UHFFFAOYSA-N 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000002076 α-tocopherol Substances 0.000 description 1
- 235000004835 α-tocopherol Nutrition 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
- A23D9/007—Other edible oils or fats, e.g. shortenings, cooking oils characterised by ingredients other than fatty acid triglycerides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Public Health (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Medicinal Chemistry (AREA)
- Reproductive Health (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Medical Informatics (AREA)
- Epidemiology (AREA)
- Gynecology & Obstetrics (AREA)
- Pregnancy & Childbirth (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Endocrinology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Botany (AREA)
- Nutrition Science (AREA)
- Biotechnology (AREA)
- Alternative & Traditional Medicine (AREA)
- Medicinal Preparation (AREA)
Abstract
The application discloses application of plant fruit oil in preparation of medicines and health-care foods for preventing ovarian damage caused by radiotherapy and chemotherapy, wherein the plant fruit oil is sea buckthorn seed oil. The application finds that the sea buckthorn seed oil has a good prevention effect on ovarian injury caused by radiotherapy and chemotherapy. The sea buckthorn seed oil can prevent mouse ovary damage and estrus cycle disorder caused by radiotherapy and chemotherapy, and can effectively improve ovary reserve function and the like. The invention provides a basis for directly utilizing the seabuckthorn seed oil to prevent ovarian injury caused by radiotherapy and chemotherapy.
Description
Technical Field
The application relates to the technical field of application of plant fruit oil, in particular to application of plant fruit oil in preparing medicines and health-care foods for preventing ovarian damage caused by radiotherapy and chemotherapy.
Background
Although generally, the tumor incidence in men is higher than in women, the tumor incidence in women increases very rapidly with the current change of lifestyle. Recently, there has been a report: in the next two decades, the incidence of cancer in uk women is 6 times higher than in men, and this phenomenon is not only in the west but also in our countries, so that the terms breast cancer, cervical cancer, ovarian cancer, gastric cancer, etc. are no longer unknown to us, and the burden of the neoplastic disease is very heavy both for the individual patient and for the whole society. However, for tumors, the treatments that we can select are often very limited. At present, patients who can be treated by surgery mostly adopt surgical resection assistance to carry out radiotherapy and chemotherapy, and the radiotherapy and chemotherapy become the biggest choice for patients who lose surgical chances. Although the radiotherapy and chemotherapy improve the symptoms of patients to a certain extent and prolong life, the damage of radiotherapy and chemotherapy to other organs of the body is always an inevitable problem in clinic. The ovary is the most sensitive part to chemotherapy and radiation in the female reproductive system, and the ovary can be damaged by large-dose and long-time radiotherapy and chemotherapy, so that the ovary is damaged. In recent years, due to the rapid increase of the number of tumors suffered by women, ovarian functions of patients are damaged when the patients receive chemoradiotherapy, perimenopause symptoms such as menstrual disorder and amenorrhea occur, even infertility can be caused to bring great influence on physical and mental health of the women, but for the influence, the restraint is often not done clinically because of the lack of effective preventive medicines. The world health organization estimates that two-fifths of the 1400 ten thousand cancers diagnosed per year are preventable. Therefore, it is important to take a series of measures to protect the structure and function of the ovary before applying a treatment for damage to the ovary. At present, the effect of ovarian protection measures is not ideal, and better medicines are required to be searched for to deal with the damage, so that how to protect the ovarian function of patients undergoing radiotherapy and chemotherapy from being damaged is paid more and more attention by researchers.
Currently, the most studied protective drugs include gonadotropin releasing analogues, oral contraceptives, SIP, amifostine and other antioxidants. However, gonadotropin releasing analogues such as triptorelin and oral contraceptives belong to hormone drugs, and symptoms such as hot flashes, night sweats and the like caused by osteoporosis and low estrogen states can appear after long-term administration; exogenous antioxidant drugs are difficult to be popularized clinically due to factors such as high price, many side effects of drugs, uncertain long-term treatment effect and the like. Therefore, drug developers and clinicians try to find a comprehensive, effective, cheap and convenient drug which can reduce and prevent the damage of ovaries caused by radiotherapy and chemotherapy.
The Chinese medicine seabuckthorn has a long medicinal history in China, and the seabuckthorn has the functions of relieving cough and asthma, promoting blood circulation and removing blood stasis and the like which are recorded in the four medical dictionaries. More than 400 active ingredients of seabuckthorn fruit mainly comprise: nutrient and active ingredients such as flavonoids, crude fibers, polysaccharides, amino acids, vitamins, trace elements, carotene, proteins, organic acids, chlorophyll and the like; can be widely applied to various fields of national economy such as food, medicine, light industry, spaceflight, agriculture, animal husbandry, fishery and the like. The sea-buckthorn seed oil is the main component of sea-buckthorn fruit, is rich in nutrition, contains more than 100 active substances such as various vitamins, fatty acids, trace elements, phytosterol, linolein, sea-buckthorn flavone, alpha-tocopherol, superoxide and the like and various amino acids required by a human body according to determination, has the effects of resisting inflammation, promoting granulation, repairing ulcer, inhibiting tumor and resisting cancer, and particularly has obvious curative effects on cerebral arteriosclerosis, angina, hyperlipidemia and the like. So far, no prevention effect of sea buckthorn seed oil on ovarian injury caused by radiotherapy and chemotherapy is reported.
Disclosure of Invention
The application discloses application of plant fruit oil in preparing medicines and health-care foods for preventing ovarian damage caused by radiotherapy and chemotherapy, namely application of sea buckthorn seed oil in preparing medicines and health-care foods for preventing ovarian damage caused by radiotherapy and chemotherapy.
According to the embodiment of the application, the application of the plant fruit oil in preparing the medicine for preventing the ovarian damage caused by the radiotherapy and chemotherapy is provided, and the plant fruit oil is the sea buckthorn seed oil. .
According to the embodiment of the application, the application of the plant fruit oil in preparing the health-care food for preventing the ovarian damage caused by the radiotherapy and chemotherapy is provided, and the plant fruit oil is the sea buckthorn seed oil.
Furthermore, the dosage form of the medicine comprises decoction, soft capsules, dripping pills, emulsions, tablets, lotions, liniments, ointments or suppositories.
Further, the dosage form of the health food comprises decoction, soft capsules, dripping pills, emulsions, tablets, lotions, liniments, ointments or suppositories.
The application proves that the seabuckthorn seed oil has new functions completely different from the functions described above for the first time: the seabuckthorn seed oil has good prevention effect on ovarian injury caused by radiotherapy and chemotherapy. The sea buckthorn seed oil can prevent mouse ovary damage and estrus cycle disorder caused by radiotherapy and chemotherapy, and can effectively improve ovary reserve function and the like. The application provides a basis for directly utilizing the seabuckthorn seed oil to prevent ovarian injury caused by radiotherapy and chemotherapy.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a design of an experiment of the present application;
FIG. 2 is a technical roadmap for the present application;
FIG. 3 shows the H & E staining results of paraffin sections of the ovarian tissues of various groups of mice;
FIG. 4 is the vaginal secretion observation results of the mice in each group;
FIG. 5 is a graph of the change in estrogen E2 levels in groups of mice;
FIG. 6 shows the results of immunohistochemical AMH expression in ovarian tissues of various groups of mice;
FIG. 7 shows the change in serum AMH content of each group of mice.
Detailed Description
The application provides an application of plant fruit oil in preparing a medicine for preventing ovarian injury caused by radiotherapy and chemotherapy, wherein the plant fruit oil is sea buckthorn seed oil.
The application provides an application of plant fruit oil in preparing health food for preventing ovarian damage caused by radiotherapy and chemotherapy, wherein the plant fruit oil is sea buckthorn seed oil.
Furthermore, the dosage form of the medicine comprises decoction, soft capsules, dripping pills, emulsions, tablets, lotions, liniments, ointments or suppositories.
Further, the dosage form of the health food comprises decoction, soft capsules, dripping pills, emulsions, tablets, lotions, liniments, ointments or suppositories.
The application proves that the seabuckthorn seed oil has new functions completely different from the functions described above for the first time: the seabuckthorn seed oil has good prevention effect on ovarian injury caused by radiotherapy and chemotherapy. The sea buckthorn seed oil can prevent the ovarian injury and the disturbance of the estrus cycle of mice caused by radiotherapy and chemotherapy, and can effectively improve the ovarian reserve function and the like. The application provides a basis for directly utilizing the seabuckthorn seed oil to prevent ovarian injury caused by radiotherapy and chemotherapy.
The application aims to discuss the protective effect of the preventive application of the seabuckthorn seed oil on ovarian tissue damage caused by radiotherapy and chemotherapy, and provides an effective protection method and scientific basis for ovarian damage caused by application of a large amount of chemotherapy drugs in clinical tumor treatment.
In order to confirm that the seabuckthorn seed oil has the new functions, the following experiments are carried out.
1. Technical scheme
(1) Establishing an animal model of ovarian injury caused by chemoradiotherapy drugs:
the experimental design is shown in figure 1.
Cyclophosphamide (CY) and Busulfan (BUS) are common clinical anticancer drugs and are also representative ovarian toxicity drugs. In the experiment, an ovary injury mouse model is established by injecting 120mg/kg cyclophosphamide and 10mg/kg busulfan into the abdominal cavity of an adult female mouse at one time, and the ovary injury mouse is continuously intragastrically administrated with 2.5ml/kg seabuckthorn seed oil as a seabuckthorn seed oil prevention group (seabuckthorn seed oil protection group) every day from 7 days before modeling to 18 days after modeling; meanwhile, GnRHA triptorelin (38mg/kg) is injected subcutaneously for one time 7 days before modeling to serve as a positive control group (GnRHA group), normal saline (0.2mL/d) is used for replacing CY + BUS to be continuously perfused with stomach to serve as an ovary injury control group (model group) (the perfusion in each day is consistent with that of the model group and a sea buckthorn seed oil prevention group, and the difference caused by the perfusion operation is avoided), and a normal control group is additionally arranged, namely, a normal mouse without ovary injury is perfused with normal saline with the same volume. Materials are taken 18 days after molding, the change of the tissue morphology of the ovary is observed, the number of all-stage follicles in the ovarian tissue is counted, the levels of serum E2 and anti-mullerian hormone (AMH) are detected, and the expression level of AMH protein is detected by immunohistochemistry.
(2) Intervention in an animal model of ovarian injury induced by chemotherapeutic agents:
The experiment comprises a normal control group, a model group, a positive control group (GnRh-a (triptorelin)) and a seabuckthorn seed oil prevention group. After the model building is determined to be successful, the normal control group and the model group are intragastrically administrated with the same amount of saline water every day, and the seabuckthorn seed oil is intragastrically administrated with 2.5ml/kg (0.1ml) of seabuckthorn seed oil every day for 18 days.
(3) Experimental results monitoring and data collection
Mice in each group were tested every 4 days for changes in body weight, feeding and estrous cycles. Materials were taken 18 days after the treatment and observed for ovarian morphological changes by paraffin embedding medium section staining technique. Changes in AMH were detected by immunohistochemical techniques.
2. Technical roadmap
A technical roadmap is shown in fig. 2.
3. Main technique of
1) Animal model for establishing ovarian injury caused by chemotherapeutic drugs
Cyclophosphamide (CY) and Busulfan (BUS) are common anticancer drugs in clinic and are also representative ovarian toxicity drugs. In the research, an ovary injury mouse model is established by injecting 120mg/kg cyclophosphamide and 10mg/kg busulfan into the abdominal cavity of an adult female mouse at one time.
2) Examination of estrus cycle by smear method of vaginal cast-off cell flushing fluid
Sucking a proper amount of normal saline by a dropper, washing and sucking vaginal secretion on a glass slide at the same time point every 4 days, rapidly observing cell morphology under a microscope, and judging estrus cycle.
4. Results of the experiment
4.1 the seabuckthorn seed oil can prevent the lesion of the ovary tissue of the mouse caused by radiotherapy and chemotherapy.
Chemotherapy can directly result in damage to ovarian tissue. Previous studies have demonstrated that chemotherapy causes ovarian volume reduction, cortical atrophy, follicular loss, interstitial fibrosis and hyalinization, granulosa cells manifest acute necrotic lesions, such as a reduction in the number of granulosa cells, the appearance of numerous myelin-like structures, intracellular and intercellular edema, severe arteriosclerosis of the ovaries, marked endomysial hyperplasia and often total luminal occlusion.
As shown in fig. 3, (1) general observation: the ovary of the normal control group mouse has normal shape and ruddy color; the ovary of the model group mouse is atrophic, the surface is pale, and single or more cystic dilatation follicles can be seen on the surface of the ovary; the ovary surface of the sea buckthorn seed oil mouse is smooth and ruddy and is close to the normal tissue. (2) And (4) observing under a mirror: the ovary of a normal control group mouse can see a plurality of follicles and corpus luteum at different development stages under HE staining, granular cells are complete in shape and are regularly arranged, and the number of the granular cells is 8-9; the ovary of the model group mouse can see a plurality of primary follicles and a small number of secondary follicles, no egg cells are seen in the follicles close to the mature period, the number of visible granular cell layers is thinned, the arrangement is loose, part of the follicle falls off, no radial crown is seen, the number of visible granular cell layers can be reduced to 5-6, the corpus luteum is less, the ovarian atresia is increased and is saccular expansion, the follicular membrane cells are proliferated, the oocyte in the follicular is disappeared, and the layer of the follicular membrane cells is thicker; the ovarian tissue follicle of the positive control group is intact and is close to the normal tissue, but the interstitial substance still has obvious fibrosis; the ovary of the seabuckthorn seed oil mouse can be provided with a corpus luteum and a plurality of follicles at different development stages, the follicles close to the maturation stage can be provided with ova and a radial crown, the arrangement of granular cells is compact, the number of layers is increased, the cell layer of the follicular membrane is thin, and the shape of the ovarian follicle is close to that of a normal control group.
4.2 the sea buckthorn seed oil can prevent the disturbance of the estrus cycle of mice caused by radiotherapy and chemotherapy.
The estrus cycle refers to the time interval from the beginning of the last estrus to the beginning of the next estrus, or from the last ovulation to the next ovulation in a female mammal. The oestrus cycle is generally divided into a prophase of estrus (promestrus), an estrus, a postestrus (postestrus) and an interestrus (interval) according to several major aspects, such as the mental status of the animal, the sexual desire performance of the male by the female, the growth and development of follicles in the ovary, ovulation and corpus luteum formation, and physiological changes in the female reproductive tract (uterus, vagina, etc.). The major accessory sex organs such as ovary, uterus and vagina are constantly changing during the oestrus cycle. Having a regular estrus cycle is a marker of sexual maturity in mammals, meaning that the ovaries begin to function. In patients with ovarian damage, menstrual disorders are one of the most typical clinical manifestations, manifested as thinning menstruation, scanty menstruation and even amenorrhea. The expression in mice is indicative of a disturbance in the estrous cycle, i.e., a sustained interval.
As shown in fig. 4, the prematurity (positive control) was observed with spindle-shaped epithelial cells in clusters or alone, and with fewer leukocytes. In estrus (normal control group), a large amount of squamous epithelial cells are seen, the shape is large and irregular, no nucleus is formed, and white blood cells disappear; in the anaphase of estrus (seabuckthorn seed oil), various cell types, white blood cells, anucleate epithelial cells and fusiform epithelial cells can be seen; large numbers of leukocytes and very few fusiform epithelial cells were visible during estrus (model group). After the molding, the observation of the continuous estrus cycle every 4 days shows that: the control group of mice all have obvious 4-5 d estrus cycles and are distributed relatively evenly in each phase; the model group mice do not show obvious existence of estrus cycle, more continuous estrus intervals can be seen, and other phases of the estrus cycle show less expression, thus prompting no ovulation of the estrus cycle disorder; the sea-buckthorn seed oil group mice have obvious estrus cycles, and the recovery of the estrus cycles after the sea-buckthorn seed oil is applied is prompted. Therefore, the sea-buckthorn seed oil has a good prevention effect on the ovarian injury mouse estrus cycle disorder caused by radiotherapy and chemotherapy.
4.3 the sea buckthorn seed oil can prevent the decline of the level of the estrogen E2 in mice caused by radiotherapy and chemotherapy.
The exact mechanism of ovarian function damage caused by chemotherapeutic drugs is not completely understood at present, and the main path of ovarian damage caused by chemotherapy is considered to be probably promoting follicular cell apoptosis. It is generally believed that actively dividing cells are more sensitive to the action of chemotherapeutic drugs which may cause abnormal growth or disappearance of follicles, affecting secretion of relevant hormones, and other symptoms secondary to hormonal dyssecretion, such as decreased estrogen secretion which may feedback to hypothalamic GnRH. Increased secretion of pituitary gonadotropins (FSH, LH), which in turn accelerates the development of primary follicles into growing follicles, so that the growing follicles are destroyed again by the chemotherapeutic drugs, forming a vicious circle, eventually leading to the end of ovarian failure with thirst from the primary follicle reserve.
As shown in fig. 5: the levels of estrogen E2 in the serum of mice in the normal control group, the sea buckthorn seed oil group and the positive control group are similar and are all obviously higher than those in the model group, and the sea buckthorn seed oil group is slightly higher than that in the positive control group, which further proves that the sea buckthorn seed oil is a better medicament for preventing ovarian injury caused by radiotherapy and chemotherapy and has definite curative effect.
4.4 the seabuckthorn seed oil can prevent the hypofunction of the ovary reserve of mice caused by radiotherapy and chemotherapy.
AMH is secreted by granulosa cells of pre-antral follicles and antral follicles, inhibits the growth of the follicles, prevents the premature consumption of the follicles, and preserves the reserve of ovaries, so that the AMH is the best method for detecting the reserve function of the ovaries under different clinical conditions at present. When follicular granulosa cells are abundant and ovarian function is better, the level of AMH will be at a higher level. Conversely, as follicular granulosa cells gradually decrease, ovarian function gradually deteriorates and the level of AMH also gradually decreases. AMH is used as the best index for evaluating the ovarian reserve function, and the higher AMH index indicates that the larger the stock of the ova, the stronger the fertility is relatively. Decreased AMH suggests that the ovaries are aging, indicating a decline in female fertility.
Immunohistochemistry was used to detect AMH expression in ovarian tissue. As shown in fig. 6, there was slight yellow staining around the follicles and between granulosa cells in the normal control group, demonstrating AMH expression; the model group had little visible yellow-stained parts; in the positive control group and the seabuckthorn seed oil group, large areas of yellow stain are formed around the follicles and among granular cells, which indicates that the AMH is highly expressed in the ovaries of the two groups of mice. It can be seen that the yellow-staining fraction in the seabuckthorn oil group was nearly equal to the positive control group, which tells us that seabuckthorn oil had nearly the same efficacy as triptorelin in ovarian reserve.
4.5 the sea buckthorn seed oil can prevent the reduction of the content of mouse AMH hormone caused by radiotherapy and chemotherapy.
The present application uses an enzyme-linked immunosorbent assay (ELISA) to determine serum AMH levels. As shown in figure 7, compared with the model group, the seabuckthorn seed oil can obviously improve the expression of AMH in blood plasma of mice with ovarian injury caused by chemotherapy, and further proves that the seabuckthorn seed oil has a good protective effect on ovarian reserve function reduction caused by chemotherapeutic drugs.
As can be seen from the above experimental results,
(1) the oleum Hippophae can prevent change of ovary tissue structure of mouse caused by radiotherapy and chemotherapy, and improve ovary tissue morphology
(2) The oleum Hippophae can prevent the disturbance of estrus cycle of mice caused by radiotherapy and chemotherapy, and maintain normal physiological cycle of mice
(3) The oleum Hippophae can be used for preventing hypoestrogenic level of mice caused by radiotherapy and chemotherapy
(4) The oleum Hippophae can prevent the damage of ovary reserve function of mice caused by radiotherapy and chemotherapy
(5) The similarity of radiotherapy and chemotherapy to the ovarian injury of mice, and the seabuckthorn seed oil has the prevention and protection effects on the ovarian dysfunction caused by radiotherapy and chemotherapy.
Other embodiments of the present application will be apparent to those skilled in the art from consideration of the specification and practice of the application disclosed herein. This application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the application and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains. It is intended that the specification and examples be considered as exemplary only, with a true scope and spirit of the application being indicated by the following claims.
It is to be understood that the present application is not limited to what has been described above, and that various modifications and changes may be made without departing from the scope thereof. The scope of the application is limited only by the appended claims.
Claims (4)
1. Application of oleum Hippophae in preparing medicine for preventing ovarian injury caused by radiotherapy and chemotherapy is provided.
2. Application of oleum Hippophae in preparing health food for preventing ovarian injury caused by radiotherapy and chemotherapy is provided.
3. The use of claim 1, wherein the medicament is in the form of decoction, soft capsule, dripping pill, emulsion, tablet, lotion, liniment, ointment or suppository.
4. The use of claim 2, wherein the health food is in the form of decoction, soft capsule, dripping pill, emulsion, tablet, lotion, liniment, ointment or suppository.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010525333.1A CN111529557B (en) | 2020-06-10 | 2020-06-10 | Application of plant fruit oil in preparation of medicines and health foods for preventing ovarian injury caused by radiotherapy and chemotherapy |
| US17/344,008 US11911426B2 (en) | 2020-06-10 | 2021-06-10 | Use of a plant in preparation of medicines and health products for preventing and treating ovarian injury |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010525333.1A CN111529557B (en) | 2020-06-10 | 2020-06-10 | Application of plant fruit oil in preparation of medicines and health foods for preventing ovarian injury caused by radiotherapy and chemotherapy |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111529557A CN111529557A (en) | 2020-08-14 |
| CN111529557B true CN111529557B (en) | 2022-06-28 |
Family
ID=71969729
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010525333.1A Active CN111529557B (en) | 2020-06-10 | 2020-06-10 | Application of plant fruit oil in preparation of medicines and health foods for preventing ovarian injury caused by radiotherapy and chemotherapy |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111529557B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007023012A (en) * | 2005-07-20 | 2007-02-01 | Reino:Kk | Skin cream containing hippophae oil and enzymes |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110075139B (en) * | 2019-05-29 | 2021-07-30 | 宁夏医科大学 | Application of sea-buckthorn seed oil in medicine for preventing polycystic ovarian syndrome |
-
2020
- 2020-06-10 CN CN202010525333.1A patent/CN111529557B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007023012A (en) * | 2005-07-20 | 2007-02-01 | Reino:Kk | Skin cream containing hippophae oil and enzymes |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111529557A (en) | 2020-08-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Liu et al. | Cardioprotection activity and mechanism of Astragalus polysaccharide in vivo and in vitro | |
| CN110025642B (en) | Application of sea buckthorn berry in medicine for treating polycystic ovarian syndrome | |
| CN110075139B (en) | Application of sea-buckthorn seed oil in medicine for preventing polycystic ovarian syndrome | |
| CN104800773B (en) | A kind of Chinese medicine composition and preparation method thereof for preventing new milking cow uterine disease | |
| US20170246230A1 (en) | Pharmaceutical composition for treating infertility, and preparation method and use thereof | |
| Li et al. | Protective effect of Clinopodium chinense (Benth.) O. Kuntze against abnormal uterine bleeding in female rats | |
| CN111529557B (en) | Application of plant fruit oil in preparation of medicines and health foods for preventing ovarian injury caused by radiotherapy and chemotherapy | |
| CN110123851B (en) | Application of sea buckthorn seed oil in medicine for treating polycystic ovarian syndrome | |
| CN111481579B (en) | Application of plant fruit pulp in preparing medicine and health food for preventing ovarian damage caused by radiotherapy and chemotherapy | |
| CN111544459B (en) | Application of plant fruit oil in preparation of medicine and health food for treating ovarian injury caused by radiotherapy and chemotherapy | |
| JP7257399B2 (en) | Methods for increasing embryo implantation rates in female patients with polycystic ovary syndrome | |
| CN101332282B (en) | Traditional Chinese medicine composition for treating gynecologic diseases from kidney deficiency and genitals coldness, and its preparation method | |
| CN111643532B (en) | Application of plant fruit pulp in preparing medicine and health food for treating ovarian injury caused by radiotherapy and chemotherapy | |
| Singh et al. | Contemporary approaches in the management of uterine leiomyomas | |
| CN101919991B (en) | Drug composition for treating cervical cancer and preparation method thereof | |
| CN111905022B (en) | Medicine for promoting ovulation and preparation method thereof | |
| CN110123850B (en) | Application of sea-buckthorn berry in medicine for preventing polycystic ovarian syndrome | |
| CN102125671A (en) | Traditional Chinese medicinal composition for treating gynaecopathia caused by deficiency of the kidney and cold and preparation method thereof | |
| US11911426B2 (en) | Use of a plant in preparation of medicines and health products for preventing and treating ovarian injury | |
| CN113616720B (en) | Medicine for preventing and treating early threatened abortion and preparation method thereof | |
| CN112870277B (en) | Traditional Chinese medicine composition for preventing and treating recurrent abortion | |
| CN114748551B (en) | Traditional Chinese medicine composition capable of improving ovarian function injury caused by chemotherapeutic drugs, and preparation method and application thereof | |
| Ismail et al. | Normalisation of human chorionic gonadotrophin (hCG) levels in a patient with partial molar pregnancy with a uterine mass without chemotherapy: impact of using herbal remedies | |
| US11058737B2 (en) | Use of Euycoma longifolia extract in alleviating symptoms and/or conditions associated with hormonal imbalance in females | |
| CN103251860B (en) | One treats dermopathic pharmaceutical composition and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |