CN110025642B

CN110025642B - Application of sea buckthorn berry in medicine for treating polycystic ovarian syndrome

Info

Publication number: CN110025642B
Application number: CN201910455720.XA
Authority: CN
Inventors: 何瑞; 于普光; 李广永; 马春梅; 李研; 王燕蓉; 孙苗; 塔拉; 喻晨; 胡亚楠; 吴紫瑶
Original assignee: Ningxia Medical University
Current assignee: Ningxia Medical University
Priority date: 2019-05-29
Filing date: 2019-05-29
Publication date: 2021-12-07
Anticipated expiration: 2039-05-29
Also published as: CN110025642A

Abstract

The application discloses a new application of sea buckthorn berry, namely the application of the sea buckthorn berry in preparing a medicament for treating polycystic ovarian syndrome and the application of the sea buckthorn berry in preparing a health food for treating the polycystic ovarian syndrome. The experimental result shows that the seabuckthorn fruit pulp has comprehensive treatment effect on the polycystic ovarian syndrome. The sea buckthorn berry not only can improve polycystic ovarian syndrome mouse ovarian polycystic lesion, but also can correct the estrus cycle disorder presented by the polycystic ovarian syndrome, maintain the AMH level, recover the normal reserve of the ovary, effectively reduce the blood sugar and relieve the obesity state in the aspect of endocrine metabolism. The application provides scientific basis for directly utilizing the seabuckthorn pulp to treat the polycystic ovarian syndrome.

Description

Application of sea buckthorn berry in medicine for treating polycystic ovarian syndrome

Technical Field

The application relates to the technical field of application of sea buckthorn berry, in particular to application of sea buckthorn berry in a medicine for treating polycystic ovarian syndrome.

Background

Polycystic ovary syndrome (PCOS) is an endocrine disease characterized primarily clinically by insulin resistance, androgen elevation, and ovarian polycystic ovary. The hyperandrogenism and ovarian dysfunction exhibited by PCOS, often accompanied by glycolipid metabolic disorders such as hyperinsulinemia and dyslipidemia, ultimately leads to the development of obesity, type 2 diabetes and cardiovascular disease, with PCOS patients at a 4-fold higher risk of diabetes than healthy people. Obese PCOS patients exhibit more severe insulin resistance, abdominal fat deposition and hyperandrogenism, with over 60% of PCOS patients being overweight or obese and about 70% exhibiting insulin resistance.

PCOS is one of the most common reproductive and endocrine disorder diseases of women of reproductive age, the morbidity of the PCOS in China is 12.5% -15%, the PCOS can cause infertility of the women, and the health of the women is seriously harmed. The etiology of PCOS is not well defined, and its central pathological mechanisms are insulin resistance and hyperandrogenism. The main clinical manifestations are insulin resistance, hyperandrogenism, hyperlipidemia, obesity and chronic inflammation, accompanied by abnormal menstruation and ovarian polycystic lesions. Thus, PCOS treatment is focused on reducing body weight, improving insulin resistance and hyperandrogenism, and restoring ovarian function.

Current treatment for PCOS mainly includes surgical treatment and drug treatment. The operation is ovarian perforation, but because the treatment effect is poor, the complications are many, and the ovarian reserve can be influenced, the operation is rarely adopted at present; the drug therapy is mainly divided into four symptomatic treatments: firstly, treating hyperandrogenism-Daying-35; regulating menstrual cycle-oral contraceptive, progestogen; ③ insulin resistance therapy-metformin; clomiphene citrate as ovulation-promoting medicine. However, each drug is only a symptomatic treatment, the curative effect is poor, most drugs are hormone drugs, and complications are more, so that an effective comprehensive treatment drug for treating the PCOS is lacked at present.

The Chinese medicine Seabuckthorn (Seabuckthorn) has a long medicinal history in China, and is recorded in the modern Chinese medicine dictionary: the properties and application of sea buckthorn: sour in flavor, neutral in nature, entering lung and stomach meridians. Has effects in moistening lung, nourishing stomach, promoting salivation, quenching thirst, promoting digestion, and relieving diarrhea, and can be used for treating lung dryness, chronic cough, cough with little phlegm, gastric and duodenal ulcer, fever, body fluid deficiency, xerostomia, and diabetes. More than 400 active ingredients of seabuckthorn fruit mainly comprise: vitamins, flavonoids, triterpenes, steroids, oils and fatty acids, amino acids, proteins, phenols, organic acids, volatile oils, trace elements, phospholipids, 5-hydroxytryptamine, saccharides, etc. The seabuckthorn fruit pulp is the main component of seabuckthorn fruits and is obtained by squeezing through a physical method, the seabuckthorn fruit pulp is rich in nutrition and contains more than 100 active substances such as various vitamins, fatty acids, trace elements, phytosterol, linolein, seabuckthorn flavone, alpha-tocopherol, superoxide and the like and various amino acids required by a human body according to determination. To date, no therapeutic effect of seabuckthorn pulp on polycystic ovarian syndrome (reproductive field) has been reported.

Disclosure of Invention

The application discloses a new application of sea buckthorn berry, namely the application of sea buckthorn berry in a medicine and a health food for treating polycystic ovarian syndrome, and solves the problem that an effective comprehensive treatment medicine is lacked for treating polycystic ovarian syndrome at present.

According to an embodiment of the application, there is provided use of seabuckthorn pulp in the manufacture of a medicament for the treatment of polycystic ovary syndrome.

According to an embodiment of the present application, there is provided a use of seabuckthorn pulp for the manufacture of a health food for the treatment of polycystic ovary syndrome.

Furthermore, the dosage form of the medicine comprises soft capsules, dripping pills, emulsions, tablets, lotions, liniments, ointments or suppositories.

Further, the dosage form of the health food comprises soft capsules, dripping pills, emulsions, tablets, lotions, liniments, ointments or suppositories.

The application proves that the seabuckthorn pulp has a comprehensive treatment effect on the polycystic ovarian syndrome for the first time. The sea buckthorn berry not only can improve polycystic ovarian syndrome mouse ovarian polycystic lesion, but also can correct the estrus cycle disorder presented by the polycystic ovarian syndrome, maintain the AMH level, recover the normal reserve of the ovary, effectively reduce the blood sugar and relieve the obesity state in the aspect of endocrine metabolism. The application provides a foundation for directly utilizing the seabuckthorn pulp to treat the polycystic ovarian syndrome.

Drawings

In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

FIG. 1 is a technical roadmap for the present application;

FIG. 2 shows the H & E staining results of paraffin sections of the ovarian tissues of various groups of mice;

FIG. 3 is the vaginal secretion observation results of the mice in each group;

FIG. 4 is the results of morphological observation of the mice in each group;

FIG. 5 is a line graph showing the change in body weight of mice in each group;

FIG. 6 is a bar graph of the body weight gain ratio of the mice in each group;

FIG. 7 shows the results of glucose tolerance experiments in mice of each group;

FIG. 8 is the area under the curve of the glucose tolerance test of each group of mice;

FIG. 9 shows the AMH expression results of the mice in each group.

Wherein, 1-normal control group, 2-Sham group (model group), 3-positive control group, and 4-fructus Hippophae pulp group.

Detailed Description

The embodiment of the application provides application of seabuckthorn fruit pulp in preparing a medicament for treating polycystic ovarian syndrome.

The embodiment of the application provides application of sea buckthorn berry pulp in preparing health food for treating polycystic ovarian syndrome.

In order to confirm that the seabuckthorn fruit pulp has the new functions, the following experiments are carried out:

1. technical scheme

(1) Preparing sea-buckthorn pulp:

collecting fresh mature fructus Hippophae, cleaning, air drying, wrapping with double-layer gauze, and physically squeezing to obtain pulp.

(2) Establishing a mouse PCOS animal model:

letrozole is an aromatase inhibitor that inhibits the conversion of ovarian androgens to estrogens, thereby causing hyperandrogenism. High-fat feeding can cause insulin resistance. ICR mice were given high-fat feeding plus gavage of letrozole at 200 ug/day for 21 days, which resulted in a PCOS model of insulin resistance and hyperandrogenism, with estrogenic cycle disturbances, persistent intervals, and elevated blood glucose (this is the PCOS standard model building approach accepted in the industry). Meanwhile, a group of normally bred ICR mice was set as a normal control group.

(3) Intervention in a mouse PCOS animal model:

the experiment is divided into a normal control group, a model group, a metformin (metform) positive control group and a seabuckthorn fruit pulp treatment group (pulp group). On the 21 st day after molding, after confirming that molding was successful, the normal control group and the model group were administered with saline of the same amount per day by intragastric gavage, the metformin (metformine) positive control group was administered with metformin of 50mg/kg (0.1ml) per day by intragastric gavage, and the seabuckthorn berry pulp treatment group (pulp group) was administered with seabuckthorn berry pulp of 5ml/kg per day by intragastric gavage for 28 days.

(4) Experimental results monitoring and data collection

Mice in each group were tested and recorded for weight, feeding and estrous cycle changes every 2 days.

Blood glucose was measured every 7 days after fasting and glucose tolerance OGTT test was given 27 days after the administration treatment.

Materials were taken 28 days after the dosing treatment. Ovarian morphological changes were observed by paraffin embedding medium section staining technique. Changes in AMH were detected by immunohistochemical techniques.

2. Technical route map

A technical roadmap is shown in fig. 1.

3. Main techniques

(1) Establishment of mouse PCOS animal model

Letrozole is an aromatase inhibitor that inhibits the conversion of ovarian androgens to estrogens, thereby causing hyperandrogenism. High-fat feeding can cause insulin resistance. High fat feeding plus gavage letrozole, 200 ug/day, given for 21 days, may cause a PCOS model of insulin resistance and hyperandrogenism.

(2) Oral sugar tolerance test (OGTT)

The mice are fasted for 16-18h, the glucose is gavaged for 3g/kg body weight, and the blood sugar is measured and recorded at the time points of 0min, 15min, 30min, 60min, 90min and 120 min.

(3) Examination of estrus cycle by vaginal cast-off cell washing smear method

Sucking a proper amount of normal saline by a dropper, washing and sucking vaginal secretion on a glass slide at the same time point every 2 days, rapidly observing cell morphology under a microscope, and judging estrus cycle.

4. Results of the experiment

4.1 fructus Hippophae pulp can improve polycystic ovary pathological changes of mouse with polycystic ovary syndrome and improve ovary morphology

Follicular dysgenesis and anovulation are the core features of reproductive dysfunction of patients with PCOS, and the fundamental manifestations of follicular dysgenesis of patients with PCOS are excessive follicular recruitment, obstructed follicular selection and domination, and arrested follicular development, resulting in anovulation. The disease detection result shows that the ovary is changed like a polycycle, the number of corpus luteum is obviously reduced, the leucocyte is thickened, and a plurality of follicles and atretic follicles with different degrees are seen under the leucocyte. The white membrane of the ovary is obviously collagenized and wraps the ovary in a lamellar way to finally form vacuole-like changes, so that the ovulation is abnormal. In the research, the application finds that the sea buckthorn berry can be used for remarkably treating PCOS mouse ovarian polycystic lesion and improving the ovarian morphology.

General observation: the ovary of the normal control group mouse is normal in shape and ruddy in color; the ovary of the mouse of the Sham model group is bigger, the surface is pale, and single or more cystic follicles can be seen on the surface of the ovary; the ovarian appearance of the metformin control group and the seabuckthorn berry group are both close to the normal control.

As shown in fig. 2, histomorphometric results: the ovary of a normal control group mouse can see a plurality of follicles and corpus luteum at different development stages under H & E staining, granular cells are complete in shape and are regularly arranged, and the number of the granular cells is 8-9; the ovary of the mouse of the Sham model group can see a plurality of primary follicles and a small number of secondary follicles, which is similar to the situation that no egg cells are seen in the follicles in the maturation stage, the number of layers of visible granular cells is thinned, the arrangement is loose, part of the granular cells fall off, the corpus luteum is less formed, the follicular atresia is increased and is saccular expansion, and the cell layer of the follicular membrane is thicker; the positive control group (metformin) ovarian morphology was close to the normal control group; the ovary of the mouse with the seabuckthorn berry pulp group can see corpus luteum and follicles at different development stages, the follicles close to the maturation stage can see ova and radial crowns, granular cells are arranged closely, the number of layers is increased, the cell layer of the follicular membrane is thin, and the shape of the follicle membrane is close to that of a normal control group.

The experiment proves that the ovary morphology of the mice in the seabuckthorn berry treatment group is obviously recovered, and the recovery effect is close to that of the normal group and the metformin positive control group.

4.2 the sea buckthorn pulp can remarkably recover the estrus cycle of the PCOS mouse

Having a regular estrus cycle is a marker of sexual maturity in mammals, meaning that the ovaries begin to function. Menstrual disorder is one of the most typical clinical manifestations of patients with polycystic ovarian syndrome, manifested as infrequent menstruation, scanty menstruation and even amenorrhea. The expression in mice is indicative of a disturbance in the estrous cycle, i.e., a sustained interval. The application finds that the sea buckthorn berry pulp can effectively treat the disorder of the estrus cycle of the PCOS mouse, so that the mouse can recover the normal regular estrus cycle.

As shown in fig. 3, the observation of the continuous estrus cycle every 2 days after the molding revealed that: the normal control group of mice has an obvious 4-5 d estrus cycle and the distribution of each phase is relatively balanced; the Sham model group mice are in a continuous estrus interval from 7 days of model building, have disordered estrus cycles and are in a continuous estrus interval 16 days of model building, and no estrus, early estrus and late estrus appear any more, so that no ovulation is prompted; the positive control (metformin) was improved over the Sham model group, but was less pronounced than the hippophae pulp group; when the mice in the seabuckthorn fruit pulp group are treated for 7 days, the estrus cycle is gradually recovered until the treatment for 14 days, the normal estrus cycle is recovered, and the regular estrus cycle appears, so that the recovery of the estrus cycle after the application of the seabuckthorn fruit pulp is prompted.

Therefore, the sea-buckthorn berry has a good treatment effect on PCOS mouse estrous cycle disorder.

4.3 the use of Hippophae rhamnoides pulp for reducing obesity in PCOS mice

About 50% -70% of patients with PCOS are overweight or obese, and clinical studies show that patients with PCOS combined obesity have more severe symptoms such as insulin resistance and hyperphoremia, so that weight loss is an effective way to relieve the symptoms of PCOS. The present application finds that sea buckthorn pulp can significantly reduce the weight of PCOS mice.

As shown in fig. 4, the body type of the model group mice was significantly larger than that of the normal control group, while the body type of the mice was close to normal after the metformin positive control and the hippophae rhamnoides pulp treatment were given, and the body type of the mice given the hippophae rhamnoides pulp group was more slim.

As shown in fig. 5, the body weight of the mice was continuously monitored and a line graph was drawn, and it was seen that the body weight of the mice in the model group after the administration of high fat feeding and letrozole was significantly increased compared to the normal control group, while the body weight of the mice in the metformin and hippophae rhamnoides pulp treated group was slowly increased, and even before the material was obtained, the mice were close to the control group and no significant obesity occurred.

As shown in fig. 6, the initial body weight of the mouse was subtracted from the pre-body weight of the mouse during the experimental period to obtain the body weight gain of the mouse, and then divided by the initial body weight of the mouse to obtain the body weight gain ratio of the mouse. It can be seen that the weight gain of both groups of mice treated with metformin and hippophae rhamnoides pulp was close to that of the normal control group. While the body weight of the model group mice increased significantly.

4.4 the fructus Hippophae pulp can improve hyperglycemia of PCOS mouse, and relieve insulin resistance

Insulin resistance is one of the most important manifestations of PCOS, and the application finds that the seabuckthorn fruit pulp has good effects of improving impaired glucose tolerance and reducing blood sugar.

As shown in fig. 7 and 8, after fasting for 16 hours, each group of mice was given high glucose and subjected to an Oral Glucose Tolerance Test (OGTT). After the model group which is fed with high fat and is provided with letrozole, the glucose tolerance of the mice is obviously worsened, and the blood sugar is far higher than that of the normal control group. But the glucose tolerance is obviously improved after the metformin and the seabuckthorn fruit pulp are given. The improvement of the seabuckthorn fruit pulp on blood sugar is almost not different from the action of the traditional hypoglycemic agent metformin. The seabuckthorn fruit pulp is proved to have very good effect of improving the impaired glucose tolerance of PCOS mice.

4.5 fructus Hippophae pulp can increase ovary reserve function and AMH content in ovary tissue

Studies have shown that the root cause of reduced fertility with increasing age is associated with a decrease in the number of follicles recruitable in the ovary and a decrease in the quality of the ova, and thus a decrease in fertility potential is also referred to as a decrease in ovarian reserve function. Anti-Mullerian hormone (AMH) is one of the female reproductive regulators currently of great interest. The AMH is secreted by ovarian follicles growing at the early stage of the ovary, participates in the growth and development of the ovarian follicles, is closely related to the functions of the ovary, can accurately reflect the reserve capacity of the ovary, is secreted by granulosa cells of the antral follicle and the antral follicle, inhibits the growth of the ovarian follicles, prevents the premature consumption of the ovarian follicles, and preserves the reserve capacity of the ovary. AMH is therefore one of the most efficient and direct factors for assessing ovarian reserve function. The present application finds that the hippophae rhamnoides pulp also significantly increases the expression of AMH in PCOS mouse ovaries.

As shown in fig. 9, the AMH protein is mainly expressed in cytoplasm, envelope and nucleus of granulosa cells of ovarian tissue sections, and has a brown-yellow color. As shown in the figure, compared with the normal control group, the expression of AMH in the hippophae rhamnoides pulp group is obviously increased, even more than that of the metformin positive control group, and the AMH is mainly distributed in the area where the granular cells are located; the expression of the Model group (Model group) AMH was reduced as compared with the normal group. This indicates that the hippophae rhamnoides pulp can increase the content of AMH in ovarian tissue, thereby increasing the reserve function of the ovary.

The present application has discovered that seabuckthorn pulp has a similar therapeutic effect on PCOS compared to metformin. The medicine can treat insulin resistance, effectively reduce the weight of PCOS, recover regular estrus cycle, improve the ovarian functional state and the like, and is an effective comprehensive medicine for treating PCOS. Moreover, the sea-buckthorn is a pure plant, has no toxic or side effect and is superior to other chemical drugs.

As can be seen from the above experimental results,

(1) the sea buckthorn berry can obviously improve PCOS mouse ovary polycystic lesion and restore ovary morphology.

(2) The sea buckthorn pulp can obviously restore the estrus cycle of the PCOS mouse and restore normal ovulation.

(3) The hippophae rhamnoides pulp can reduce obesity in PCOS mice.

(4) The sea buckthorn berry pulp can obviously improve the hyperglycemia state of a PCOS mouse and reverse insulin resistance.

(5) The fructus Hippophae pulp can increase AMH, and improve the storage function of ovary of PCOS mouse.

Other embodiments of the present application will be apparent to those skilled in the art from consideration of the specification and practice of the application disclosed herein. This application is intended to cover any variations, uses, or adaptations of the invention following, in general, the principles of the application and including such departures from the present disclosure as come within known or customary practice within the art to which the invention pertains. It is intended that the specification and examples be considered as exemplary only, with a true scope and spirit of the application being indicated by the following claims.

It is to be understood that the present application is not limited to what has been described above, and that various modifications and changes may be made without departing from the scope thereof. The scope of the application is limited only by the appended claims.

Claims

1. Application of fructus Hippophae pulp as single active ingredient in preparing medicine for treating polycystic ovary syndrome is provided.

2. The use of claim 1, wherein the medicament is in a dosage form selected from the group consisting of a soft capsule, a drop pill, an emulsion, a tablet, a lotion, a liniment, an ointment, and a suppository.