CN111529549A

CN111529549A - Antibacterial and antiviral high-valence electrolytic silver ion solution and preparation method thereof

Info

Publication number: CN111529549A
Application number: CN202010395234.6A
Authority: CN
Inventors: 王平康; 李治通; 王瑞; 王安亭; 张克勋; 张琦炜
Original assignee: Luoyang Guanyin Biotechnology Co ltd
Current assignee: Luoyang Guanyin Biotechnology Co ltd
Priority date: 2020-05-12
Filing date: 2020-05-12
Publication date: 2020-08-14
Anticipated expiration: 2040-05-12
Also published as: CN111529549B

Abstract

The invention belongs to the technical field of pharmaceutical preparations, and relates to an antibacterial and antiviral high-valence electrolytic silver ion solution and a preparation method thereof. The antibacterial and antiviral high-valence electrolytic silver ion solution takes ultrapure water as a solvent, and contains positive divalent silver ions, a complexing agent and a coordination agent; the concentration of the positive divalent silver ions is 25-200 mg^‑1(ii) a The concentration of the complexing agent is 0.01-2.0 g.kg^‑1(ii) a The concentration of the complexing agent is 0.02-1.2 g.kg^‑1(ii) a The pH value of the high-valence electrolytic silver ion solution is 8.0-10.0; wherein the complexing agent is EDTA disodium of analytical grade; the complexing agent was an analytically pure grade of 25% ammonia. The positive divalent silver ions are generated in an electrolytic bath by an electrolytic method, have good compatibility with normal saline, can be used for in vivo antibacterial and antiviral treatment, and have very good stability.

Description

Antibacterial and antiviral high-valence electrolytic silver ion solution and preparation method thereof

Technical Field

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to an antibacterial and antiviral high-valence electrolytic silver ion solution and a preparation method thereof.

Background

Silver ion products supplied in the current market are classified into two types, one is a silver nitrate system, and the other is a nano silver system; the valence of silver ions in a silver nitrate system is basically one and is very sensitive to light, so the stability of a silver nitrate solution is poor, white precipitates appear quickly under indoor common light to lose efficacy, a silver nitrate product can only be used for in-vitro antibacterial disinfection and cannot be used for in-vivo antibacterial treatment, because human blood contains about 90 percent of normal saline (namely a sodium chloride solution with the concentration of 0.9 percent), the normal saline contains a large amount of chloride ions, the silver ions in the silver nitrate solution react with the chloride ions in the blood to quickly generate silver chloride white precipitates to embolize blood vessels to cause dangerous complications, and the basic reason that the silver nitrate system cannot enter an organism through venous blood vessels to kill bacteria and viruses is up to now; the nano silver is a novel antibacterial product which is formed in recent years, is not silver ions and is fine silver crystal particles with the particle size of 10-120 nanometers, Lara H, Mori Y and the like report in 2011, the nano silver can release silver ions from 111 crystal faces and 100 crystal faces of crystal grains in aqueous solution, and the good effect of inhibiting viruses such as HIV, HBV, influenza virus H1N1 and the like is achieved; 6 professors such as the medical college of university of Dalian Lian and Chinese academy of sciences in 2009, human disease focus laboratories and the molecular immunopharmacology laboratory Wangyun Huahuangning reported in the modern preventive medicine at stage 21: CC (toxicity of nano-silver to C8166 cells (leukemia T cells))₅₀105.73mg.kg^-1EC for directly killing HIV₅₀Is 12.08mg.kg^-1The action mechanism is mainly to inhibit the replication of HIV by direct virucidal, but the nano-silver isThe particle size of the silver particles is usually in the range of 15-120 nm, and once entering into the body, the nano silver particles are viewed as foreign matters by levels of phagocytic cells and are quickly worded (as shown in fig. 11), so that immune cells are killed by the nano silver particles while nano silver is removed, the immune system of the body is rapidly broken down, bacteria and germs are naturally not damaged, and therefore, the nano silver is a hundred of harmful effects and nothing of the harmful effects when entering into the body, which is a main reason why the nano silver product cannot treat an AIDS model in animal experiments for many years.

The strong antibacterial and antiviral properties of the silver-based antibacterial agent are accepted by wide scholars at home and abroad, but the current silver-based antibacterial agent cannot be used for antibacterial and antiviral treatment in a body, which is the biggest defect.

It is worth pointing out that although silver ion has definite antiviral action in vitro, it has antiviral action in vivo; no one can make scientific reasoning answers so far; the main reason is that all silver ion products sold in the market at present cannot safely enter the body through vein vessels, so that experimental research on in vivo antivirus cannot be carried out.

Disclosure of Invention

The invention aims to provide an antibacterial and antiviral high-valence electrolytic silver ion solution, a preparation device and a preparation method thereof, aiming at the defects of the prior art, the high-valence electrolytic silver ion solution has good compatibility with normal saline, can not react with chloride ions in the normal saline to generate white silver chloride precipitate, can be used for in-vivo antibacterial and antiviral treatment, and has very good stability.

The invention adopts the following technical scheme for achieving the purpose:

an antibacterial and antiviral high-valence electrolytic silver ion solution takes ultrapure water as a solvent, and contains positive divalent silver ions, a complexing agent and a complexing agent; the concentration of the positive divalent silver ions is 25-200 mg^-1(ii) a The concentration of the complexing agent is 0.01-2.0 g.kg^-1(ii) a The concentration of the coordination agent is 0.02-1.2 g.kg^-1(ii) a The above-mentionedThe pH value of the high-valence electrolytic silver ion solution is 8.0-10.0; wherein the complexing agent is EDTA disodium of analytical grade; the complexing agent is 25% ammonia water of analytical purity grade.

The ultrapure water is water with the resistivity reaching 18M omega cm (25 ℃).

A preparation device of antibacterial and antiviral high-valence electrolytic silver ion solution comprises an electrolytic bath; the electrolytic bath is an insulator, one end of the electrolytic bath is an anode plate made of a high-purity silver plate, and the other end of the electrolytic bath is a cathode plate made of a stainless steel plate; the back of the negative plate is sealed with an ultrasonic oscillator, and an labyrinth partition plate for controlling the water flow direction is arranged in the electrolytic bath; the center of the electrolytic tank is provided with a stirrer, and the anode plate and the cathode plate are respectively connected with the anode and the cathode of the direct current power supply.

The preparation material of the electrolytic cell is engineering plastics or a metal material covered with an insulating material layer, and the electrolytic cell can be a cuboid, an ellipsoid or a cylinder.

The purity of the high-purity silver plate in the prepared anode plate is 99.9-99.999%, the thickness of the high-purity silver plate is 0.3-5.0 mm, and the area of the anode plate is 3.0-30% of the inner surface area of the electrolytic cell.

The cathode plate is composed of a 304 stainless steel plate, the thickness of the stainless steel plate is 0.5-3 mm, and the area of the stainless steel plate is 3.0-20% of the inner surface area of the electrolytic cell.

The power of ultrasonic oscillator is 50 ~ 200W, and ultrasonic oscillator's frequency is 20 ~ 60KHz, and quantity is 2 ~ 8, seals at the negative plate back.

The labyrinth partition plate is made of engineering plastics and is 1-3 cm thick, the height of the labyrinth partition plate is 3-10 cm higher than the liquid level, the gap of the partition plate is 5-30 cm, and the gap of the partition plate is 15-25% of the width of the partition plate;

the motor power of the stirrer is 1-10 kW, the variable-frequency speed regulation is realized, the rotating speed range is 50-1500 revolutions, and the stirrer blade is made of engineering plastics.

The voltage of the direct current power supply is 10-200V, the current is 0.5-30A, and the direct current power supply is constant in voltage and constant in current.

A preparation method of antibacterial and antiviral high-valence electrolytic silver ion solution comprises the following specific steps:

(1) injecting ultra-pure water into the electrolytic cell, wherein the distance between the horizontal plane and the upper end surface of the electrolytic cell is 5-15 cm;

(2) starting the stirrer, slowly increasing the rotating speed, and keeping the water temperature at 20-25 ℃ all the time in the subsequent reaction process based on the highest rotating speed when the water surface does not splash and does not overflow⁰C；

(3) Adding a complexing agent and a complexing agent into the electrolytic bath, and stirring to fully dissolve the complexing agent and the complexing agent;

(4) after the complexing agent and the complexing agent are fully dissolved, a direct-current power supply and an ultrasonic oscillator are started to electrolyze silver atoms on a high-purity silver plate to obtain monovalent silver ions, and the monovalent silver ions obtained by electrolysis enter water under the attraction of a cathode electric field and then undergo a coordination reaction with the ammonia water serving as the complexing agent to generate a silver diammine coordination compound taking divalent silver ions as a core; the silver diammine coordination compound and the complexing agent EDTA disodium are subjected to coordination reaction to generate an amine carboxyl silver diammine coordination compound solution; in the reaction process, the concentration of the positive divalent silver ions in the amine carboxyl diammine silver complex solution is detected every 1 hour, and when the concentration of the positive divalent silver ions is 25-200 mg/kg^-1Turning off the direct-current power supply and the ultrasonic oscillator, and only keeping the stirrer to stir at a low speed of less than 200 revolutions for aging treatment for 48-96 hours to obtain a stable amine carboxyl diammine silver complex solution, namely the antibacterial and antiviral high-valence electrolytic silver ion solution;

(5) after aging, packaging and encapsulating the product, sealing in a light-tight or weak light-transmission clean container, and storing in shade, wherein the container is preferably made of HDPE, PP, glass and 304 stainless steel.

Compared with the prior art, the antibacterial and antiviral high-valence electrolytic silver ion solution and the preparation method thereof provided by the invention have the following outstanding advantages and effects:

(1) the high-valence electrolytic silver ion solution has good compatibility with normal saline, 5% glucose solution, 75% alcohol, 25% hydrogen peroxide and 84 disinfectant, always exists in a state of silver ion coordination compound, does not generate precipitate, and is colorless and transparent; the virus is characterized in that the virus is parasitic in cells, general medicines are ineffective to the virus, but the high-valence electrolytic silver ion solution is a novel silver ion product which can form a coordination compound with chloride ions in physiological saline, the performance is very stable, the high-valence electrolytic silver ion solution can safely enter a body through an intravenous drip way, the high-valence electrolytic silver ion solution can reach places where saline can reach, saline exists in capillaries, saline also exists in cells, and no matter the virus is distributed in blood or hidden in the cells, the silver ions can chase after.

(2) The silver ions in the high-valence electrolytic silver ion solution are high-valence silver ions with positive valence and bivalent valence, and the high-valence electrolytic silver ion solution is characterized in that the high-valence electrolytic silver ion solution is obtained by an electrolysis method in an electrolytic tank, and has no nitrate component, which is incomparable with other silver ion products.

(3) The high-valence electrolytic silver ion solution is completely different from nano silver, the high-valence electrolytic silver ion solution is silver ions, is not silver particles, cannot be phagocytosed by the kidney and the heart like metal ions such as calcium, magnesium, iron and the like in blood, cannot be precipitated with physiological saline in blood, and can maximally exert good effects of antibiosis, antivirus and anticancer in blood;

(4) the redox potential of the silver ions in the high-valence electrolytic silver ion solution is as high as 1.987v, which is obviously higher than that of monovalent silver ions by 0.798v, and the redox potential of divalent silver ions is more than 300 times of monovalent silver ions, so that the high-valence electrolytic silver ion solution has high activity, less dosage and slight toxicity.

(5) The high-valence electrolytic silver ion solution is very stable, the indoor shelf life in a sealed container can reach more than 24 months, and the high-valence electrolytic silver ion solution can be stably stored for more than 6 months under normal light even if the high-valence electrolytic silver ion solution is placed on an indoor table in a transparent container.

(6) The high-valence electrolytic silver ion solution of the invention not only can resist bacteria and viruses, but also can resist cancers, and experiments show that the high-valence electrolytic silver ionsThe concentration of the solution is 1.0mg.kg^-1Can kill the lung cancer cell KY-30 (see figure 10), and has much lower concentration than the existing metal anticancer cisplatin in clinic.

(7) The high-valence electrolytic silver ion solution has excellent antiviral performance, and the concentration of the high-valence electrolytic silver ion solution is 12 mg/kg according to 2020 detection of microbiological research institute in Guangzhou city^-1When the virus is used, 75.45% of H1N1 virus can be killed within half an hour (see the attached figures 14 and 15).

(8) The high-valence electrolytic silver ion solution has almost no toxicity when being orally taken, and according to the detection of 2019 of Henan Zhongke union detection service Co., Ltd, the acute oral administration median lethal dose of the high-valence electrolytic silver ion solution to KM male and female mice is more than 5000mg/kg of body weight, so that the high-valence electrolytic silver ion solution is practically nontoxic (see attached figure 17).

(9) The high-valence electrolytic silver ion solution is injected into an animal body through vein, and the toxicity is slight through continuous observation for 10 days, animal blood biochemical indexes and animal lung, liver and kidney tissue pathological section observation (see table 2 and attached figure 18).

(10) The high-valence electrolytic silver ion solution has good compatibility with gastric juice (containing acidic components) and intestinal juice (containing alkaline components) (see attached figures 5 and 6), and the characteristic also determines that the high-valence electrolytic silver ion solution can enter a body through an oral way, so that bacteria and viruses in a digestive tract and digestive tract tumors with active proliferation are conveniently killed, and if the high-valence electrolytic silver ion solution is used in drinking water of animals, the high-valence electrolytic silver ion solution also has a quite good control effect on avian influenza and swine fever.

(11) The surfaces of bacteria and viruses are mostly proteins, the pH of the solution is relatively high, so the bacteria and the viruses are mostly negatively charged, silver ions are positively charged, the bacteria and the viruses are rapidly combined in a detour way, and the bacteria and the viruses cannot avoid the pursuit of the silver ions as long as the surfaces of the bacteria and the viruses have the proteins.

Drawings

FIG. 1 is a schematic view of the construction of an electrolytic cell of the present invention;

FIG. 2 is a photograph showing the addition of a high-valence electrolytic silver ion solution and silver nitrate to 0.9% physiological saline, respectively;

FIG. 3 is a photograph of a 5-10% glucose solution added with a high valence electrolytic silver ion solution;

FIG. 4 is a photograph of a solution of high valence electrolytic silver ions in 75% alcohol and in 25% hydrogen peroxide;

FIG. 5 is a photograph showing the high valence electrolytic silver ion solution and silver nitrate separately added to dilute hydrochloric acid;

FIG. 6 is a photograph showing a comparison of the high valence electrolytic silver ion solution and silver nitrate to which 2.0% sodium hydroxide is added;

FIG. 7 is a microscopic morphology of erythrocytes after 24h of adding a high-valence electrolytic silver ion solution to human blood;

FIG. 8 is a photograph showing the high valence electrolytic silver ion solution and Japanese electrolytic silver after adding 2.0% sodium hydroxide, respectively;

FIG. 9 is a bacteriostatic experimental graph of a high-valence electrolytic silver ion solution;

FIG. 10 is a graph showing the experiment of killing lung cancer cell KY-30 by high valence electrolytic silver ion solution;

fig. 11 is a graph of a graphite particle configured for a cellular channel on a side of a channel;

FIG. 12 is a diagram of a high valence electrolytic silver ion solution placed indoors for 1 year (left) and 2 years (right);

FIG. 13 is a color change precipitate plot after cisplatin solution was diluted 5 times and placed indoors for 1 month;

FIG. 14 is the test report 1 of the killing experiment of high valence electrolytic silver ion solution to influenza coronavirus;

FIG. 15 is the test report 2 of the high valence electrolytic silver ion solution for killing influenza coronavirus;

FIG. 16 is a report of various basic data measurements of a high valence electrolytic silver ion solution;

FIG. 17 is a toxicity test report of a high valence electrolytic silver ion solution taken orally;

FIG. 18 is a report of pathological section of animal lung and liver and kidney tissue cytotoxicity after intravenous injection of high valence electrolytic silver ion solution;

FIG. 19 is a photograph showing the case where a high-valence electrolytic silver ion solution is added to 84 disinfectant.

In the figure: 1. the device comprises an electrolytic cell, 2, an anode plate, 3, a cathode plate, 4, an ultrasonic oscillator, 5, a labyrinth separator, 6, a stirrer, 7 and a direct current power supply.

Detailed Description

The present invention is further illustrated by the following examples, which are intended to be purely exemplary and are not intended to be limiting, and all such modifications and alterations are intended to be included within the scope of the invention, as defined by the appended claims.

The first embodiment is as follows:

adding 400-600 kg of ultrapure water into a small electrolytic cell, starting a stirrer, rotating at a speed of 210-530 revolutions to ensure that water flow does not splash and does not overflow, ensuring that water flow does not splash when passing through a labyrinth formed by a partition plate, slowly adding 0.11-0.480 kg of EDTA disodium serving as a complexing agent under stirring, slowly adding 0.15-0.430 kg of ammonia water serving as a complexing agent and accounting for 25% of a complexing agent after 10 minutes, stirring for 15 minutes, starting a direct-current power supply, adjusting the voltage to 5-36V and the current to 0.5-1.3A, starting 2-4 ultrasonic oscillators, controlling the frequency of the oscillators to be 30-50 KHz, ensuring that no obvious white precipitate exists on the surface of a cathode in the electrolytic reaction process, and controlling the water temperature to be 20-25 ℃ in the whole preparation process⁰And C, sampling and detecting the silver ion concentration once per hour until the concentration of the positive divalent silver ions in the solution reaches 25-30 mg^-1When the direct current power supply and the ultrasonic oscillator are turned off, the reaction duration of the system is not subject to rigid regulation, the comprehensive quality of the designed high-valence electrolytic silver ion solution is guaranteed, the product concentration and the product stability are guaranteed to reach the standard (the quality guarantee period is more than 24 months), it is very important to properly adjust the voltage, the current, the water temperature and the rotating speed of the stirrer during electrolysis, and if the voltage is too high and the current is too high, the water temperature exceeds 25^oC, the rotating speed is too high, the concentration of silver ions can be increased too fast, but the stability is poor, so that the silver ions and the silver ions must be considered at the same time, and the comprehensive quality is guaranteed; when the DC power supply is turned offAnd immediately reducing the rotating speed of the stirrer to about 180 revolutions after the ultrasonic oscillator, running at a low speed for aging for two days, wherein the aging aims to increase the stability of silver ions, and after two days, subpackaging and encapsulating the stabilized amino carboxyl diamino silver coordination compound, and storing in a shade place to obtain the formal product of the high-valence electrolytic silver ion solution.

As shown in figure 1, the preparation device of the antibacterial and antiviral high-valence electrolytic silver ion solution comprises an electrolytic bath 1; the electrolytic bath 1 is an insulator, one end of the electrolytic bath is an anode plate 2 made of a high-purity silver plate, and the other end of the electrolytic bath is a cathode plate 3 made of a stainless steel plate; the back of the negative plate 3 is sealed with an ultrasonic oscillator 4, and an labyrinth separator 5 for controlling the water flow direction is arranged in the electrolytic bath; the center of the electrolytic tank 1 is provided with a stirrer 6, and the anode plate 2 and the cathode plate 3 are respectively connected with the anode and the cathode of a direct current power supply 7.

The preparation material of the electrolytic cell 1 is engineering plastics or a metal material covered with an insulating material layer, and the electrolytic cell can be a cuboid, an ellipsoid or a cylinder.

Example two:

adding 800-1100 kg of ultrapure water into a medium-sized electrolytic tank, starting a stirrer, rotating at a speed of 180-500 revolutions, taking the principle that water flow does not splash and does not overflow, taking the principle that water flow does not splash when passing through a labyrinth formed by a partition plate, slowly adding 0.19-0.88 kg of EDTA disodium as a complexing agent under stirring, slowly adding 0.27-0.80 kg of ammonia water with 25% of a complexing agent after 10 minutes, stirring for 15 minutes, starting a direct-current power supply, adjusting the voltage to 15-45V, the current to 1.1-3.0A, starting 4-6 ultrasonic oscillators, controlling the frequency of the oscillators to be 30-50 KHz, taking the principle that no obvious white or brown black precipitate exists on the surface of a cathode in the electrolytic reaction process as the whole preparation process, and controlling the water temperature to be 20-25⁰And C, sampling and detecting the silver ion concentration once per hour until the silver ion concentration reaches 45-60 mg/kg^-1When the direct current power supply and the ultrasonic oscillator are turned off, the reaction duration of the system is not subject to rigid regulation, the comprehensive quality of the designed high-valence electrolytic silver ion solution is guaranteed, the product concentration and the product stability are guaranteed to reach the standard (the quality guarantee period is more than 24 months), it is very important to properly adjust the voltage, the current, the water temperature and the rotating speed of the stirrer during electrolysis, and if the voltage is too high and the current is too high, the water temperature exceeds 25^oC, the rotating speed is too high, the concentration of silver ions can be increased too fast, but the stability is poor, so that the silver ions and the silver ions must be considered at the same time, and the comprehensive quality is guaranteed; when the direct current power supply and the ultrasonic oscillator are turned off, the rotating speed of the stirrer is immediately reduced to about 180 revolutions, the stirrer is operated at low speed for three days, the aging is carried out for three days, the aging aims to increase the stability of silver ions, the stabilized amine carboxyl diammine silver coordination compound is packaged and encapsulated after three days, and the product is stored in a shade place, namely the formal product of the high-valence electrolytic silver ion solution.

Example three:

adding 1800-2100 kg of ultrapure water into a large electrolytic tank, starting a stirrer, rotating at a speed of 160-550 r, and slowly adding 0.45-1.60 kg of complexing agent EDTA disodium under stirring to ensure that water flow does not splash and overflow and does not splash when passing through an labyrinth formed by a partition plate, and slowly adding 0.55-100 ℃ of ammonia water containing 25% of complexing agent after 10 minutes1.65kg, stirring for 15 minutes, starting a direct current power supply, adjusting the voltage to be 28-65V, the current to be 2.5-5.8A, starting 6-8 ultrasonic oscillators, controlling the frequency of the oscillators to be 30-50 KHz, controlling the water temperature to be 20-25 ℃ in the whole preparation process based on no obvious white or brownish black precipitate on the surface of a cathode in the electrolytic reaction process⁰And C, sampling and detecting the silver ion concentration once per hour until the silver ion concentration reaches 80-100 mg/kg^-1When the direct current power supply and the ultrasonic oscillator are turned off, the reaction duration of the system is not subject to rigid regulation, the comprehensive quality of the designed high-valence electrolytic silver ion solution is guaranteed, the product concentration and the product stability are guaranteed to reach the standard (the quality guarantee period is more than 24 months), it is very important to properly adjust the voltage, the current, the water temperature and the rotating speed of the stirrer during electrolysis, and if the voltage is too high and the current is too high, the water temperature exceeds 25^oC, the rotating speed is too high, the concentration of silver ions can be increased too fast, but the stability is poor, so that the silver ions and the silver ions must be considered at the same time, and the comprehensive quality is guaranteed; when the direct current power supply and the ultrasonic oscillator are turned off, the rotating speed of the stirrer is immediately reduced to about 200 revolutions, the stirrer is operated at a low speed for aging for four days, the aging aims to increase the stability of silver ions, and the stabilized amine carboxyl diammine silver complex is packaged and encapsulated after four days and stored in a shade place, thus obtaining the formal product of the high-valence electrolytic silver ion solution.

FIG. 2 is a compatibility experiment of high valence electrolytic silver ion solution with physiological saline:

taking a national medicine standard-size physiological saline (0.9% sodium chloride injection) sold in a pharmacy of 100ml specification 2 bottles, and injecting the first bottle with the concentration of 50mg.kg^-11.0ml of high-valence electrolytic silver ion solution stock solution (the conventional use concentration is 5-50 mg/kg)^-1) 1.0ml of 1% silver nitrate solution (with the conventional use concentration of 0.1-1.0%) is injected into the second bottle, indoor observation is continuously carried out for 24 hours under normal light, if the solution is still colorless transparent solution, the phase property is judged to be qualified, and if white precipitate is separated out, the solution is judged to be unqualified; the result is shown in figure 2, the solution of high valence electrolytic silver ions is colorless and transparent when added on the left, and white precipitate is generated when added with silver nitrate on the right, thereby indicating high valence electrolytic silver ionsThe sub-solution has excellent compatibility with normal saline, which lays a foundation for the high valence electrolytic silver ion solution to enter the body through intravenous drip.

To further verify the compatibility of the high valence electrolytic silver ion solution with physiological saline, we examined the compatibility with a spectrophotometer while visually observing the clarity of the solution: referring to the clarity inspection method of the solution in China, a 722 type visible spectrophotometer is selected to measure the absorbance and transmittance of a sample at the wavelength of 550nm (see table 1).

1. The reference sample is ultrapure water;

2. the comparative samples were 0.9% saline and 2mm thick clear glass slides;

3. the sample (1) to be tested is prepared by adding a small amount of physiological saline into a large amount of high-valence electrolytic silver ion solution, the sample (2) to be tested is prepared by adding a small amount of high-valence electrolytic silver ion solution into a large amount of physiological saline, the blood volume of the adult is about 5000ml, if 50ml of high-valence electrolytic silver ion solution stock solution is intravenously dripped every time, the volume is equal to 100 times of the blood volume, namely, 1ml of high-valence electrolytic silver ion solution is added into 100ml of physiological saline, and intravenous drip is performed at 1 drop per second; and only the positive and negative interfaces (a small amount of physiological saline added with a large amount of high-valence electrolytic silver ion solution and a small amount of high-valence electrolytic silver ion solution added with a large amount of physiological saline) can reach the light transmittance of the transparent glass with the light transmittance of more than 2mm, and the transparent glass can be judged to be a qualified product.

4. When the compatibility of strong base (sodium hydroxide solution) with high-valence electrolytic silver ion solution and silver nitrate is detected, not only small amount of high-valence electrolytic silver ion solution and silver nitrate are added into a large amount of sodium hydroxide solution, but also small amount of sodium hydroxide solution is added into a large amount of high-valence electrolytic silver ion solution and silver nitrate, and the condition can be judged to be qualified only when the front interface and the back interface are colorless and transparent.

The values of the portions of the samples examined with the spectrophotometer are shown in the following table:

attached table 1 data of spectrophotometer detection data

FIG. 3 is a compatibility experiment of high valence electrolytic silver ion solution and glucose solution:

filling 100ml of commercial grade 5% and 10% glucose solutions into 1 bottle respectively, injecting 10ml of high-valence electrolytic silver ion solution stock solution with the concentration of 50mg.kg < -1 >, continuously observing for 24h under indoor normal light, judging that the compatibility is qualified if the solution is still colorless and transparent, and judging that the solution is unqualified if white precipitates are separated out; as shown in the attached figure 3, the high valence electrolytic silver ion solution is colorless and transparent no matter added into the isotonic or hypertonic glucose solution, i.e. the high valence electrolytic silver ion solution has good compatibility with the glucose solution, which brings convenience for intravenous drip of the high valence electrolytic silver ion solution for the hypertension patients and also enlarges the application range of the high valence electrolytic silver ion solution.

FIG. 4 is a compatibility experiment of high valence electrolytic silver ion solution with alcohol and hydrogen peroxide:

taking 2 reagent bottles of 30ml, respectively adding 25ml of 75% ethanol and 25% hydrogen peroxide solution, respectively adding 1.5ml of high-valence electrolytic silver ion solution stock solution, observing under indoor light, judging that the compatibility is qualified if the solution is still colorless transparent solution, and judging that the compatibility is unqualified if white precipitate is separated out; the result is shown in figure 4, when the high valence electrolytic silver ion solution is added into 75% ethanol and 25% hydrogen peroxide, the solution is still colorless and transparent, which shows that the high valence electrolytic silver ion solution has excellent compatibility with ethanol and hydrogen peroxide. The result indicates that if the high-valence electrolytic silver ion solution is added into alcohol or hydrogen peroxide, the disinfection time of the alcohol or hydrogen peroxide can be obviously prolonged, and the combination of the alcohol and the hydrogen peroxide can complement the rapid disinfection and the long-time disinfection.

FIG. 5 is a compatibility experiment of high valence electrolytic silver ion solution with dilute hydrochloric acid solution:

2 bottles of analytically pure grade 3.0% strength hydrochloric acid solution, 25ml each, were added 50mg.kg to the first bottle^-1High chemical combination of concentration1.0ml of original solution of silver ion solution is electrolyzed, and 50mg.kg is added into a second bottle^-11.0ml of silver nitrate solution with the concentration is continuously observed under indoor common light for 24 hours, if the silver nitrate solution is still colorless transparent solution, the compatibility is judged to be qualified, and if white precipitate is separated out, the silver nitrate solution is judged to be unqualified; the result is shown in figure 5, the high valence electrolytic silver ion solution is colorless and transparent when diluted hydrochloric acid is added, and the silver nitrate is precipitated when diluted hydrochloric acid is added, which shows that the high valence electrolytic silver ion solution has good compatibility with the diluted hydrochloric acid, and lays a foundation for the oral application of the high valence electrolytic silver ion solution (hydrochloric acid in gastric juice).

FIG. 6 is a valence determination and a strong base compatibility experiment of a high valence electrolytic silver ion solution:

2 reagent bottles of 30ml are taken, and 50mg is added into one reagent bottle^-128ml of high valence electrolytic silver ion solution, 50mg of one silver ion solution is added^-1Adding 0.5ml of 2% sodium hydroxide into 2 test tubes respectively, observing under indoor normal light, judging that the compatibility is qualified and has high valence if the solution is still colorless transparent solution, and judging that the compatibility is unqualified and has valence of only 1 if white precipitate is separated out; the result is shown in figure 6, the solution is colorless and transparent when the left high-valence electrolytic silver ion solution is added with strong alkali, and a large amount of precipitate is separated out when the right silver nitrate solution is added with strong alkali, thereby showing that the high-valence electrolytic silver ion solution has excellent compatibility with alkali liquor, which lays a foundation for the intestinal application of the high-valence electrolytic silver ion solution (the intestinal tract has dilute alkali liquor). Meanwhile, the valence of the high-valence electrolytic silver ion solution is proved to be 2.

The judgment principle of the silver ionization valence (reported on the network) is as follows: monovalent silver ion Ag¹⁺Belongs to soft acid, is difficult to hydrolyze in solution, can generate silver oxide precipitate to be separated out when meeting strong alkali, and has divalent silver ions Ag²⁺It belongs to boundary acid, when adding strong alkali, it can not produce silver oxide precipitate, and after adding strong alkali liquor, it can conveniently judge the valence of silver ion. Monovalent silver ion Ag¹⁺Has an oxidation-reduction potential of 0.798v and a divalent silver ion Ag²⁺Oxidation reduction potential ofThe efficiency and the reduction potential of the silver ion electrolytic solution are more than 300 times of those of monovalent silver salt (such as silver nitrate) and simple substance silver (such as nano silver) up to 1.987v, so that the activity and the physicochemical property of the high-valence electrolytic silver ion solution are much better than those of the silver nitrate and the nano silver.

FIG. 7 is a compatibility experiment of high valence electrolytic silver ion solution with erythrocytes:

adding 3ml of fresh human blood which is just extracted into a glass tube with anticoagulant, adding 0.5ml of high-valence electrolytic silver ion solution stock solution with the batch number of 190217, wherein the volume ratio of the high-valence electrolytic silver ion solution to the human blood is 1: 6 (equivalent to injecting the stock solution of the high-valence electrolytic silver ion solution into a blood vessel of an adult by nearly 1000ml), shaking uniformly, taking 0.1ml, spreading on a glass slide, observing whether red blood cells are hemolyzed or not by using an optical microscope, and judging the compatibility of the high-valence electrolytic silver ion solution and the red blood cells; the results are shown in figure 7, the red blood cells have normal morphology, no change after being placed for 24 hours, and no hemolysis, which indicates that the high valence electrolytic silver ion solution has good compatibility with the red blood cells.

FIG. 8 is a differential experiment between a high valence electrolytic silver ion solution and a Japanese electrolytic silver ion solution:

at a concentration of 25mg.kg in 28ml^-1The high valence electrolytic silver ion solution and 28ml concentration of the high valence electrolytic silver ion solution are 25mg^-11.0ml of 2.0% sodium hydroxide solution was added to each of the Japanese electrolytic silver ion solutions, and the silver ionization valence of a colorless and transparent sample was judged to be +2 valence, and the silver ionization valence of a discolored sample was judged to be +1 valence, when the samples were continuously observed for 24 hours. As shown in the attached FIG. 8, the high valence electrolytic silver ion solution with strong base added on the left side is colorless and transparent, and the valence is judged to be +2, and the Japanese electrolytic silver ion solution with strong base added on the right side has precipitate, and is judged to be + 1. According to the publicity reports of Japan Rizhang Kabushiki Kaisha, the purity of silver materials is improved from 99.9% to 99.999999% by an electrolytic method, then the high-purity silver materials are added with nitric acid and nitrate and are filtered by a molecular sieve to form a high-stability silver nitrate solution, the essence of the silver nitrate solution does not get rid of the scope of silver nitrate, and the stable concentration sold on the market is 25mg kg^-1And the lowest side is marked with a small character to indicate that the product will be matched withChlorine element reacts to generate white precipitate which is only used for in vitro disinfection and chemical products; the high valence electrolytic silver ion solution is a silver ion product prepared by an electrolytic method in an electrolytic cell, does not contain any nitrate component, and has stable concentration of more than 50mg.kg^-1The valence of the silver ion is +2, and the silver ion does not react with chlorine to generate white precipitate, so that the silver ion can be used in vitro and in vivo, namely the Chinese high-valence electrolytic silver ion solution is different from the Japanese electrolytic silver ion solution in nature.

FIG. 9 is a bacteriostatic experiment of high valence electrolytic silver ion solution:

entrusted with the detection of medical colleges of the university of science and technology in Henan, the method is simple; the result is shown in figure 9, the concentration of the high valence electrolytic silver ion solution is 0.25mg^-1When the antibacterial agent is used, the inhibition rate of P.g bacteria is as long as 72 hours, and the high-valence electrolytic silver ion solution has strong antibacterial capability.

FIG. 10 shows the experiment of killing lung cancer cell KY-30 with high valence electrolytic silver ion solution:

the detection method is carried out by the medical college of science and technology university in Henan, and is omitted. The results are shown in FIG. 10, and the concentration of the high valence electrolytic silver ion solution is 1.0mg.kg^-1Can kill the lung cancer cells KY-30, which is equivalent to 5.0mg of high-valence electrolytic silver ion solution (the average blood volume of an adult is about 5kg, according to the concentration of 1.0 mg.kg) dripped into the vein of an adult every time^-1The mass of the high-valence electrolytic silver ion solution is 5.0mg) is obtained by calculation, the cisplatin which is a clinically common anticancer drug is a coordination compound of heavy metal platinum, the atomic weight of the platinum is 195 which is 87 greater than the atomic weight of the silver 108, the proportion of the metal platinum in the cisplatin is 65%, and when the cisplatin is clinically used for intravenous drip of an impact therapy, the dosage can reach 240mg each time, wherein the weight of the platinum ion is 156mg which is more than 30 times of the weight of the silver ion in the high-valence electrolytic silver ion solution.

Fig. 11 is a drawing of a nano-silver configured throughput on a cell-level card:

the end result of the actions of silver nanoparticles is that they are first phagocytosed and then killed, and then expelled out of the body, resulting in a breakdown of the immune system and a massive proliferation of bacterial viruses.

FIG. 12 is a graph of stability experiment of high valence electrolytic silver ion solution:

the high valence electrolytic silver ion solution is still colorless and transparent after being placed in a room for 1 year (left) and 2 years (right), and the concentration detection is more than 95% of the concentration of the stock solution, which shows that the high valence electrolytic silver ion solution has high stability, strong tolerance to indoor common light and more than 24 months of shelf life.

FIG. 13 is a chart of stability tests of cisplatin, a commonly used anticancer agent:

the bottled cisplatin solution is diluted by 5 times and then is placed indoors for 1 month to generate discoloring precipitates, and the cisplatin is also a metal ion coordination compound and is a clinically common broad-spectrum anti-cancer drug, but the aqueous solution of the cisplatin solution has poor tolerance to light; various scholars at home and abroad prove that the silver ion solution also has good anticancer performance.

FIGS. 14 and 15 are experimental reports of the killing of influenza virus H1N1 by high-valence electrolytic silver ion solution:

the microbial research institute of Guangzhou city is entrusted to detect, and the detection is based on the disinfection technical Specification of 2002, and the disinfection detection system contains 0.5ml of H1N1 virus solution, 0.5ml of 3% bovine serum albumin and 15mg.kg of high-valence electrolytic silver ion solution^-14ml, and 5ml of the total system, wherein the albumin concentration is diluted by 10 times and is 0.3% from 3%, namely the concentration is 3000mg.kg^-1The concentration of the high valence electrolytic silver ion solution is diluted by 1.25 times, namely 12mg^-1That is, the concentration of albumin in the detection system is 250 times of the concentration of the high-valence electrolytic silver ion solution, the albumin has negative charges, the silver ions have positive charges, that is, most of the silver ions in the system are adsorbed by the albumin, and only a very small amount of the high-valence electrolytic silver ion solution participates in virus killing, however, the high-valence electrolytic silver ion solution can kill 75.45% of viruses within half an hour, and the antiviral effect of the high-valence electrolytic silver ion solution is quite strong.

FIG. 16 shows the results of various basic data tests of high valence electrolytic silver ion solutions:

the detection of the United creation detection service company Limited in the department of the south of entrusted river is carried out by a slight method; the results are shown in FIG. 16: the content, the validity period, the PH value, the mercury content, the lead content and the arsenic content of the high-valence electrolytic silver ion solution all accord with the regulations, the bacteriostatic action on staphylococcus aureus, escherichia coli and candida albicans all accord with the regulations, and the experiment on microbial pollution accords with the requirements.

FIG. 17 shows the results of animal toxicity tests when high valence electrolytic silver ion solutions were administered orally:

the detection of the United creation detection service company Limited in the department of the south of entrusted river is carried out by a slight method; the result is shown in figure 17, the high valence electrolytic silver ion solution has no toxicity when the animal orally takes the solution, which indicates that the high valence electrolytic silver ion solution can not generate obvious toxic and side effects on the organism when entering the organism through the digestive tract, and also indicates that the high valence electrolytic silver ion solution has good prevention and treatment effects on digestive tract infection and digestive tract tumor caused by bacteria and viruses, and if a trace amount of the high valence electrolytic silver ion solution is added into drinking water of the animal, the high valence electrolytic silver ion solution can have good prevention and treatment effects on avian influenza and swine fever.

The attached table 2 shows the results of the animal biochemical indicators (liver function and kidney function) after the high valence electrolytic silver ion solution is injected into the vein:

the institute of medicine of the university of science and technology of Henan is entrusted with detection. The method comprises the following steps: 8 Japanese great white rabbits were divided into 3 groups, the first group 2 was a control group without injection of drugs, the second group 3 was a low dose test group, and the third group 3 was a high dose test group. On the first day, before injection, blood is drawn from ear veins of all rabbits by 1.0ml to serve as a normal value control, and then high-valence electrolytic silver ion solution is injected from ear veins of rabbits in an experimental group, wherein the amount of pure silver ions contained in the high-valence electrolytic silver ion solution injected each time in a low-dose experimental group is 17.2ug^-1.d^-1The method is equivalent to that 20ml of high-valence electrolytic silver ion solution stock solution is injected into an adult per time, the injection amount of the high-dosage experimental group is 3 times of that of the low-dosage experimental group, and the method is equivalent to that 60ml of high-valence electrolytic silver ion solution stock solution is injected into an adult per time, the high-valence electrolytic silver ion solution stock solution is injected into the adult per time, and the animals are killed once a day, continuously for 3 days and on the 10 th day. Animals before sacrifice on day 4 and day 10Each blood is drawn again, and the blood is drawn for 3 times in total to be used as the biochemical index detection of the liver function and the kidney function of the animal; on day 10, the animals were sacrificed and the lung, liver and kidney were taken for pathological section examination.

The results are shown in the attached table 2, the 10 biochemical indexes of the experimental animals are slightly different from those of the control group, and the low-dose experimental group is also slightly different from that of the high-dose experimental group, which indicates that the intravenous vascular administration of the high-valence electrolytic silver ion solution has slight toxicity to the liver function and the kidney function of the animals under the administration dose of the experiment.

Attached table 2: animal 10 biochemical index detection value

FIG. 18 is a report of pathological section of animal lung, liver and kidney tissue cytotoxicity caused by intravenous injection of high-valence electrolytic silver ion solution:

the completion of the medical college of the science and technology university of Henan and the third subsidiary hospital of the science and technology university of Henan was entrusted. The section of the animal tissue cells is shown in FIG. 18. Before the animals in the two groups of the experimental group die by day 10, the animals have no weight loss, no disturbance in body posture, normal diet and defecation, and no difference from the animals in the control group. After the animals die, lung, liver and kidney tissues are taken for pathological section observation, and the pathological section observation shows that the lung, liver and kidney tissue sections of the animals in the high-dose experimental group and the animals in the low-dose experimental group have no obvious difference when being injected with the high-valence electrolytic silver ion solution intravenously, and have no obvious difference when being compared with the lung, liver and kidney tissue sections of the animals in the normal control group, namely, the two groups of animal tissue sections in the experimental group have no obvious pathological change. In the figure, the

reference numbers

1, 2 and 3 are lung liver and kidney tissue sections of control animals, and the

reference numbers

4, 5 and 6 are lung liver and kidney tissue sections of high-dose experimental animals. From the condition of animal lung, liver and kidney tissue sections, the intravenous administration of the high-valence electrolytic silver ion solution has low toxicity to animal lung, liver and kidney tissue organs at the administration dosage of the experiment.

FIG. 19 is a photograph of a solution of high valence electrolytic silver ions added to 84 disinfectant:

30ml of 84 disinfectant of two brands are respectively added into two reagent bottles, then 1ml of stock solution of the high-valence electrolytic silver ion solution is respectively added, after the solution is stabilized for 12 hours, the solution clarity is observed by naked eyes, and simultaneously, the absorbance and the transmissivity are detected by a spectrophotometer, the result is shown in figure 19 and the detection of the spectrophotometer in attached table 1, the two bottles are colorless transparent solutions, no precipitation exists, the detection is qualified by a photometer, the transmissivity is more than 99.8%, which shows that the high-valence electrolytic silver ion solution can be used together with the 84 disinfectant, so that the rapid disinfection and the long-term disinfection are complementary, and the disinfection time and the disinfection effect can be obviously increased.

The administration method of the high-valence electrolytic silver ion solution suggests:

1. intravenous drip

According to 0.01-0.1 mg.kg^-1Calculating the mass of silver ions, converting, accurately extracting a certain volume of stock solution of the high-valence electrolytic silver ion solution, and performing intravenous drip at the speed of 60 drops/minute under indoor common light. The method is suitable for patients with virus and bacterial infection and cancer, such as AIDS virus, hepatitis B virus, coronavirus and various kinds of mutant virus, acute pancreatitis, septicemia, burn, leukemia, hepatocarcinoma, lung cancer, renal cancer, pancreatic cancer, brain cancer, etc., and also has obvious effect on diseases caused by virulent "super bacteria" such as Escherichia coli O-157 and resistant staphylococcus (MRSA).

And oral administration

According to 0.1-0.5 mg.kg^-1Calculating the mass of silver ions, accurately extracting a certain volume of high-valence electrolytic silver ion solution stock solution after conversion, and adding into 100ml of cold boiled water for slow oral administration. The method is suitable for patients with diseases and malignant tumors caused by oral digestive tract infection by bacteria and viruses, such as acute and chronic pharyngitis, gastritis, enteritis, gastric cancer, intestinal cancer, etc. caused by helicobacter pylori. The animal can be used for preventing and treating avian influenza and swine fever.

Local injection medicine

It is mainly used for body surface, lymph node, oral cavity and maxillofacial tumor focus, and the dosage is determined by the focus volume.

Aerosol therapy

The aerosol is mainly used for preventing and treating early upper respiratory tract and lung infection caused by influenza virus, coronavirus and various mutant viruses, and can be conveniently inhaled into a household atomizer at low cost for 0.5 hour every time and 3 times a day. Intravenous drip is used when the disease is serious.

Jet therapy

It is mainly used for treating acute and chronic diseases of nasopharynx, throat and eye, and burn wound. The method can use an elephant nose bottle with the volume of about 30ml, aims at the nasal cavity, throat or eyes (eyes are struggled), sprays for 3-5 times a day, and has good curative effects on preventing and treating cold in the early stage and preventing virus from spreading through respiratory tract.

Targeted therapy

The high valence electrolytic silver ion solution stock solution is encapsulated in a high molecular microcapsule by a microcapsule technology, is injected to the center of a focus, slowly releases divalent silver ions at local parts, is mainly used for treating malignant tumors, and plays a role in quickly killing cancer cells and relieving toxic and side effects on the whole body.

Magnetic microcapsule guided therapy

The high valence electrolytic silver ion solution stock solution is encapsulated in microcapsules consisting of high molecular materials and magnetic materials, and is guided to move by a magnet on the body surface and concentrated in the center of a focus, so that the super-targeting therapeutic effect is achieved, and the super-targeting therapeutic agent is mainly used for body surface tumors, lymph cancer, oral cancer, nasopharyngeal cancer and the like.

The high-valence electrolytic silver ion solution is developed from 2016 for 3 months, has various physical and chemical properties which are greatly different from those of currently known silver nitrate, nano silver and Japanese electrolytic silver ions, does not contain any nitrate component and polymer carrier, is not a nano particle, has good compatibility with physiological saline, glucose solution, dilute hydrochloric acid, dilute alkali liquor, alcohol, hydrogen peroxide, 84 disinfectant and the like, is colorless and transparent, has no irritation, corrosiveness and drug resistance, and has the effective period of more than 2 years; the characteristics that the high-valence electrolytic silver ion solution cannot be phagocytosed by the phagocytic level and cannot react with physiological saline in blood to generate silver chloride precipitate like calcium ion and magnesium ion, determine that the high-valence electrolytic silver ion solution can enter into the body through intravenous drip so as to conveniently kill various bacteria, viruses and cancer cells with active proliferation, and is also effective on mutated bacteria and viruses with drug resistance; the characteristics are incomparable with the common silver nitrate system, the nano silver system and the electrolytic silver ion system produced in Japan.

The high valence electrolytic silver ion solution has good compatibility with gastric juice (containing acidic components) and intestinal juice (containing alkaline components), and the characteristic also determines that the high valence electrolytic silver ion solution can enter into organisms through oral administration, thereby conveniently killing bacteria and viruses in the digestive tract and digestive tract tumors with active proliferation.

80% of infectious diseases of human beings are caused by viruses, particularly AIDS, hepatitis B, SARS, coronary pneumonia and infectious diseases caused by various variant viruses, which cause serious damage to the life and the body health of human beings, and the batch production and the application of the high-valence electrolytic silver ion solution can add a new effective means and a method for treating a plurality of viral diseases.

The silver ion is positively charged, and the microbial shell is negatively charged, so that when the silver ion contacts the cell membrane of the bacteria, the silver ion can be rapidly combined with the cell membrane and enter the cell nucleus, and the intracellular protein is coagulated, denatured and necrotic. The killing effect on the protoplasm is the same as that of the intact cells, and the silver ions can be combined with the protoplasm more quickly to denature and die the protoplasm.

Silver ions can generate active oxygen, the active oxygen has oxidative decomposition capacity which is several times that of ultraviolet rays and ozone, and has extremely strong killing and decomposition effects on bacteria and viruses, and even in water without light and in a dark environment, the silver ions can also generate active oxygen and still have killing properties on the bacteria and the viruses.

The antibacterial and antiviral effects of silver ions have long been known, and the first phase of winding reported by doctor of high warrior in 2002 in "the college chemical engineering journal" 16 is: silver ion is the most abundant in waterThe low effective antibacterial concentration MED is 0.05mg^-1The concentration can meet the sanitary requirement of GB5749-85 sanitary Standard for Drinking Water.

According to the report of the United nations health administration, when the concentration of silver ions in drinking water is 0.05mg^-1When the concentration of silver ion is 0.3mg.kg, the colibacillus in water can be completely killed, and new flora can not be propagated in water within 90 days^-1It can kill most pathogenic bacteria and inactivate viruses. That is, silver ions have a strong antiviral effect at a low concentration.

Expert in digestive sciences, J Poweil, reported that one antibiotic killed 6 pathogenic organisms, while silver ions killed 650 biological and mutant recalcitrant strains.

In 2016, Beijing coordination Hospital Guohua reports that the polypeptide anti-tumor efficacy antagonized by CXCR4 and the toxicity research of nano-silver: the ability of the ancient opiating and phagocytic cells to take up large quantities of silver nanoparticles, but not silver ions, was confirmed by EDX.

Doctor huguansong, southern science and technology university, 2016 reports: the nano silver is at 1.6X10^-2And in the uM process, the breast cancer Mcf-7 cells can be killed, the killing power of the rodlike nano silver on the Mcf-7, K562 and A549 cancer cells is superior to that of the anticancer drug trichostatin, and the anticancer effect of the nano silver is also caused by silver ions released from the surface of the nano silver.

Arakawa H2001 reports that silver ions released from the surface of nano silver can be bound to DNA and RNA to form Ag-DNA and Ag-RNA complexes, and when the concentration of the silver ions is low, the silver ions are bound with N7 atoms on DNA guanine, and when the concentration of the silver ions is high, the silver ions are bound with N7 atoms on adenine to block the replication of cells, so that the cells are apoptotic. Cancer cells are abundant in blood supply, take up much higher amounts of silver ions than normal cells, and therefore also die quickly.

In 2010, Lankveld D P K et al reported that 20-110 nm silver nanoparticles were intravenously injected once a day for 5 consecutive days into rats, and that the nanoparticles disappeared rapidly after being injected into blood, and were capable of quickly removing the silver nanoparticles on the phagocytic side of the blood, regardless of the size of the silver nanoparticles, and were also killed by the silver nanoparticles.

Recordati reported in 2016 that the distribution of silver ions in rats was distinct from that of silver nanoparticles on the rat tail vein after the same dose of silver nanoparticles and silver ions were injected, and that silver nanoparticles were capable of being phagocytosed by phagocytic cells and were incapable of inducing the biological effects of silver ions in vivo, which were much different from that of silver nanoparticles.

Cisplatin, an anticancer drug consisting of heavy metal platinum, is officially approved in the United states in 1978 to be clinically outstanding, and silver ions, a silver ion drug consisting of precious metal silver, have been proved to have good anticancer effects by various scholars.

The concentration of the nano-silver for killing leukemia cells is CC₅₀105.73mg.kg^-1While the concentration of the high-valence electrolytic silver ion solution for killing the lung cancer cells KY-30 is 1.0mg.kg^-1It can be seen that the anticancer capacity of the high valence electrolytic silver ion solution is far higher than that of nano silver, and the toxicity is far lower than that of nano silver due to small dosage.

Reported in journal of pharmaceutical research by professor Sun Jianfei of Shandong province's society of pharmacy in 2017, wherein the concentration of silver ions is lower than 12.5mg.kg^-1It is non-cytotoxic. Silver is one of trace elements in human tissues, and WHO stipulates that the safety value of silver to human body is 0.05mg^-1The trace silver is harmless to human bodies, and the low content of silver ions in the bodies of people who are often infected by bacterial viruses is also one of important reasons. The poisoning dose of the silver element ingested by the human body is 60mg, and the lethal dose is 1300-6200 mg. The dosage of the high-valence electrolytic silver ion solution for intravenous drip is 10-90 ml, namely 0.5-4.5 mg, which is only 13-120 times of the toxic dosage.

After 22 burn patients of Coombs C J use sulfadiazine silver 6 hours in 1992, the maximum content of silver ions in blood can reach 310ug^-1The silver ion amount discharged from the urine of the patient is 100-400 ug every day. Silver ions can be safely discharged out of the body, are not accumulated in the body, do not cause environmental pollution, and enter when the silver sulfadiazine is used for treating burn and wound surfacesThe concentration in blood can reach 20mg.kg^-1(the concentration is 4-40 times of the concentration of the high valence electrolytic silver ion solution in common use).

Reported by professor haif in 2014 at the society of environmental mutagens in Beijing, the concentration of 5mg.kg after rat tail vein injection^-1When the silver ion content is high, the concentration of the silver ion in the blood of the rat is up to 12.5mg^-1However, the animal body weight is not changed, the micronucleus rate of bone marrow red blood cells is not obviously increased, and only certain damage is generated on chromosomes of the red blood cells.

M S Wysor 1050 mg/kg/day^-1The sulfadiazine silver (containing 30 percent of silver) is orally or subcutaneously applied to mice for one month continuously, the animals have no abnormality at the end of the experiment, and no obvious pathological change is detected in tissue sections.

Reported in journal of Chinese burn in 2004 by professor Wenzhou medical college Chen 28884, silver ions in blood of patients with II-degree burn entering through the silver-carrying auxiliary material layer can be safely discharged out of body through urine within 14 days and restored to the level before treatment, the silver metabolism in body is in a safe range, and the silver content in normal human blood is 1.54 +/-1.09 ug.kg^-1The silver content in urine is 1.0 plus or minus 0.71ug^-1The amount of the silver auxiliary materials reaches 3.88 +/-4.42 ug after 5 days^-1And 3.68 + -4.99 ug.kg^-1The silver content returns to the level before treatment on day 9 after the silver adjuvant is not used, that is, the human body has the functions of automatic metabolism and no accumulation in the body for silver ions.

Most of common salt eaten in daily life is iodized salt, the atomic weight of iodine is 126.9, which is 19.1 times larger than that of silver by 107.8, and people eat iodized salt all the year round without causing iodine accumulation in the body.

The virus is characterized in that the virus is parasitic in cells, general medicines are ineffective to the virus, but a high-valence electrolytic silver ion solution is a novel silver ion product which can form a coordination compound with chloride ions in physiological saline, saline exists in capillaries, saline also exists in cells, no matter whether the virus is distributed in blood or hidden in the cells, the silver ions can be traced, no matter how the bacteria and the virus are mutated, the tracing of the silver ions cannot be avoided as long as the surface of the virus has proteins.

The antiviral drugs sold in the current market can almost only block the replication of the virus but can not kill the virus, once the drugs are stopped, the virus can be replicated and erupted in a large amount, namely the drugs can treat the symptoms and root causes but not the root causes, but if the high-valence electrolytic silver ion solution is used in combination with the virus inhibiting drugs commonly used in the current market, the double sword combines the walls, the symptoms and the root causes are treated, and the major breakthrough is certainly generated in the field of the virus resistance!

The household ultrasonic atomizer has small volume and low price, is particularly suitable for atomizing and inhaling high-valence electrolytic silver ion solution, is very simple and suitable for preventing and treating respiratory tract and pneumonia infection caused by coronavirus, and has almost no obvious side effect.

A significant new epidemic-resisting technology is obtained in the United states, namely, one doctor in New York treats 699 patients with new crown pneumonia by using hydroxychloroquine, azithromycin and zinc sulfate in a combined mode, all the patients are cured, the effect is remarkable, of the three medicines, hydroxychloroquine is a medicine for treating malaria and has a certain antiviral effect, but is not strong, azithromycin is an antibiotic and is not effective on viruses, zinc sulfate is a metal ion medicine, zinc ions are divalent metal ions and have an antibacterial and antiviral function, and the three medicines are used in a combined mode, wherein the antiviral effect of zinc ions cannot be achieved, namely, the metal ions have strong virus killing characteristics and are real, silver ions are included, and the antibacterial and antiviral functions of the silver ions are absolutely greater than those of the zinc ions.

Most diseases in the world are caused by viruses, the treatment of viral diseases is very difficult today with the change of science and technology in the world, although many scholars at home and abroad can prove that silver ions can kill viruses in vitro, the silver ions cannot be infused into the body through veins to treat viral infectious diseases, and the batch production and application of the high-valence electrolytic silver ion solution can add a new means and method for preventing and treating the viral diseases.

Claims

1. An antibacterial and antiviral high-valence electrolytic silver ion solution is characterized in that: high valence electricityThe silver ion solution takes ultrapure water as a solvent, and the high-valence electrolytic silver ion solution contains positive divalent silver ions, a complexing agent and a coordination agent; the concentration of the positive divalent silver ions is 25-200 mg^-1(ii) a The concentration of the complexing agent is 0.01-2.0 g.kg^-1(ii) a The concentration of the coordination agent is 0.02-1.2 g.kg^-1(ii) a The pH value of the high-valence electrolytic silver ion solution is 8.0-10.0; wherein the complexing agent is EDTA disodium of analytical grade; the complexing agent is 25% ammonia water of analytical purity grade.

2. The antibacterial and antiviral high-valence electrolytic silver ion solution as claimed in claim 1, wherein: the ultrapure water is water with the resistivity reaching 18M omega cm (25 ℃).

3. The preparation device of the antibacterial and antiviral high-valence electrolytic silver ion solution is characterized in that: the preparation device comprises an electrolytic bath; the electrolytic bath is an insulator, one end of the electrolytic bath is an anode plate made of a high-purity silver plate, and the other end of the electrolytic bath is a cathode plate made of a stainless steel plate; the back of the negative plate is sealed with an ultrasonic oscillator, and an labyrinth partition plate for controlling the water flow direction is arranged in the electrolytic bath; the center of the electrolytic tank is provided with a stirrer, and the anode plate and the cathode plate are respectively connected with the anode and the cathode of the direct current power supply.

4. The apparatus for preparing antibacterial and antiviral high-valence electrolytic silver ion solution according to claim 3, wherein: the preparation material of the electrolytic cell is engineering plastics or a metal material covered with an insulating material layer, and the electrolytic cell can be a cuboid, an ellipsoid or a cylinder.

5. The apparatus for preparing antibacterial and antiviral high-valence electrolytic silver ion solution according to claim 3, wherein: the purity of the high-purity silver plate for preparing the anode plate is 99.9-99.999%, the thickness of the high-purity silver plate is 0.3-5.0 mm, and the area of the anode plate is 3.0-30% of the inner surface area of the electrolytic cell.

6. The apparatus for preparing antibacterial and antiviral high-valence electrolytic silver ion solution according to claim 3, wherein: the cathode plate is composed of a 304 stainless steel plate, the thickness of the stainless steel plate is 0.5-3 mm, and the area of the stainless steel plate is 3.0-20% of the inner surface area of the electrolytic cell.

7. The apparatus for preparing antibacterial and antiviral high-valence electrolytic silver ion solution according to claim 3, wherein: the power of ultrasonic oscillator is 50 ~ 200W, and ultrasonic oscillator's frequency is 20 ~ 60KHz, and quantity is 2 ~ 8, seals at the negative plate back.

8. The apparatus for preparing antibacterial and antiviral high-valence electrolytic silver ion solution according to claim 3, wherein: the labyrinth partition plate is made of engineering plastics and is 1-3 cm thick, the height of the labyrinth partition plate is 3-10 cm higher than the liquid level, the gap of the partition plate is 5-30 cm, and the gap of the partition plate is 15-25% of the width of the partition plate.

9. The apparatus for preparing antibacterial and antiviral high-valence electrolytic silver ion solution according to claim 3, wherein: the motor power of the stirrer is 1-10 kW, the variable-frequency speed regulation is realized, the rotating speed range is 50-1500 revolutions, and the stirrer blade is made of engineering plastics.

10. The method for preparing the antibacterial and antiviral high-valence electrolytic silver ion solution as claimed in claim 1, is characterized in that: the preparation method comprises the following specific steps: