CN111528191B - Hydraulic-driven deep-sea biological sample conservator - Google Patents
Hydraulic-driven deep-sea biological sample conservator Download PDFInfo
- Publication number
- CN111528191B CN111528191B CN202010386255.1A CN202010386255A CN111528191B CN 111528191 B CN111528191 B CN 111528191B CN 202010386255 A CN202010386255 A CN 202010386255A CN 111528191 B CN111528191 B CN 111528191B
- Authority
- CN
- China
- Prior art keywords
- reagent
- biological sample
- cabin
- sealing cover
- injection port
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000012472 biological sample Substances 0.000 title claims abstract description 66
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 114
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 87
- 238000007789 sealing Methods 0.000 claims abstract description 76
- 238000003825 pressing Methods 0.000 claims abstract description 45
- 230000007246 mechanism Effects 0.000 claims abstract description 17
- 238000003860 storage Methods 0.000 claims abstract description 4
- 238000002347 injection Methods 0.000 claims description 61
- 239000007924 injection Substances 0.000 claims description 61
- 230000000903 blocking effect Effects 0.000 claims description 17
- 230000001681 protective effect Effects 0.000 claims description 14
- 239000013535 sea water Substances 0.000 claims description 12
- 230000007704 transition Effects 0.000 claims description 10
- 230000009471 action Effects 0.000 claims description 6
- 230000008859 change Effects 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 238000013461 design Methods 0.000 claims description 4
- 239000000834 fixative Substances 0.000 claims description 4
- 230000000670 limiting effect Effects 0.000 claims description 4
- 238000009827 uniform distribution Methods 0.000 claims description 4
- 230000007547 defect Effects 0.000 claims description 3
- 230000007306 turnover Effects 0.000 claims description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 2
- 238000007599 discharging Methods 0.000 claims description 2
- 238000001125 extrusion Methods 0.000 claims description 2
- 239000000741 silica gel Substances 0.000 claims description 2
- 229910002027 silica gel Inorganic materials 0.000 claims description 2
- 238000005070 sampling Methods 0.000 abstract description 6
- 239000000523 sample Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 239000012528 membrane Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000004026 adhesive bonding Methods 0.000 description 1
- 108010066057 cabin-1 Proteins 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000009828 non-uniform distribution Methods 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K80/00—Harvesting oysters, mussels, sponges or the like
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
Abstract
The invention belongs to the technical field of marine organism sampling and storage, and relates to a water pressure driven deep sea biological sample conservator which comprises a driving device, a reagent cabin, a biological sample cabin, a combined frame, a sealing cover anti-rotation bolt and a sealing cover closing sliding mechanism, wherein the combined frame comprises an integrated pressing plate, a middle layer clamping plate, a bottom plate and a supporting rod, the driving device is arranged on the middle layer clamping plate and the bottom plate, the biological sample cabin is arranged on the integrated pressing plate and the middle layer clamping plate, the reagent cabin is communicated with the biological sample cabin through a reagent output pipeline, the sealing cover anti-rotation bolt is communicated with the driving device through a large R pipeline, and the sealing cover closing sliding mechanism is connected with the driving device through a smooth round inner wall hole pipe. The invention has light weight and small volume, can be used by carrying an underwater Robot (ROV) and an underwater Lander (Lander), effectively stores the obtained biological samples, can be independently used after being provided with a power supply, traps and collects organisms on a seabed site and fixes the organisms.
Description
Technical Field
The invention belongs to the technical field of marine organism sampling and preservation, and relates to a deep sea organism sample conservator driven by water pressure.
Background
The development and utilization of marine organism resources have become one of the competitive focuses of the oceans in the world, and the sampling of deep-sea organisms is the precondition for the development and utilization of marine organism resources. Conventional samplers do not subject the biological sample to any protective treatment and many active and genetic materials are easily degraded during the lengthy period from subsea sampling to experimental analysis, thus affecting the study.
In the course of transport of deep-sea biological samples, which is usually done according to the research objective, the samples are preserved hermetically with a suitable fixative, the best result being to preserve the samples directly in situ from the sampling. Because of the different research requirements, the ratio of fixative to sample (including seawater) is different, the highest volume ratio is required to be 9: 1.
the patent publication No. CN108142384A discloses a deep sea in situ biological fixing trap, but the structure can only collect and fix the RNA of deep sea biological samples by a trapping mode, and the proportion of the fixing liquid cannot be changed, so the applicability is low. In addition, the mode that the trapping cylinder is pressed into the reagent cabin by the motor driving can damage partial biological samples, and simultaneously, the sample storage failure is easily caused by faults or untight sealing.
Disclosure of Invention
In order to solve the technical problems in the prior art, the invention provides a deep sea biological sample conserver driven by water pressure, and the specific technical scheme is as follows.
The utility model provides a hydraulic pressure driven deep sea biological sample conservator, prevents that bolt and closing cap are closed glide machanism including drive arrangement, reagent cabin, biological sample cabin, combined frame, closing cap, combined frame includes integrated clamp plate, intermediate level splint, bottom plate and bracing piece, drive arrangement sets up on intermediate level splint and bottom plate, biological sample cabin sets up on integrated clamp plate and intermediate level splint, the reagent cabin passes through reagent output pipeline and biological sample cabin UNICOM, the closing cap prevent that the bolt passes through big R pipeline with drive arrangement UNICOM, the closing cap is closed glide machanism and is connected through smooth round inner wall hole pipe and drive arrangement.
Further, the driving device comprises a magnetic pump, the magnetic pump is provided with a first water filling port, a second water filling port and a third water filling port, and the magnetic pump drives seawater to be sequentially filled into the sealing cover anti-rotation bolt, the sealing cover closing sliding mechanism and the reagent cabin through the first water filling port, the second water filling port and the third water filling port; the first water filling port is tangent to the straight-through water outlet of the magnetic pump, the second water filling port is located at the middle of the water outlet and is arranged at the vertical position upwards, the caliber of the second water filling port is smaller than that of the first water filling port, the third water filling port is close to the pump head and is arranged at the vertical position downwards, and the caliber of the third water filling port is smaller than that of the second water filling port.
Furthermore, the sealing cover closing and sliding mechanism consists of a sliding piston, a sliding guide path, a shape-changing soft guide rod, a movable hinge sealing cover, a clamping column and a magnet; one end of the sliding guide path is connected with a second water filling port of the magnetic pump by adopting a smooth round inner wall hole pipe, the other end of the sliding guide path is connected with a round groove of the integrated pressing plate in a matching way through an O-shaped ring, and the sliding piston is arranged at one end of the shape-following flexible guide rod; the movable hinge sealing cover is hinged with the integrated pressing plate through a connecting rod, a clamping column, a magnet and a one-way valve are arranged on the movable hinge sealing cover, the clamping column is connected with the other end of the shape-changing soft guide rod, a groove is formed in the clamping column, an opening faces towards the sealing cover anti-rotation bolt when the clamping column is assembled, after the movable hinge sealing cover is completely opened, a moving mandrel bolt of the sealing cover anti-rotation bolt is inserted into the groove of the clamping column, seawater output by the magnetic pump drives a sliding piston through a second water filling port, the movable hinge sealing cover is driven to turn over through the connected shape-changing soft guide rod, and the biological sample cabin is closed; the magnets are respectively embedded in the movable hinge cover and the grooves of the integrated pressing plate, and are symmetrically distributed in an opposite manner from top to bottom; the total length of the shape-following-change soft guide rod and the sliding piston after being matched is not more than 3/4 of the sliding guide way, the diameter ratio of the diameter of the sliding piston to the inner diameter of the sliding guide way is less than 5/6, a matching gap is formed, a conservator drives the sliding piston to drive a movable hinge sealing cover connected with the shape-following-change soft guide rod under small water pressure, the movable hinge sealing cover is turned over, the sealing of the biological sample cabin is realized by the attraction of a magnet on the movable hinge sealing cover and a magnet on the integrated pressing plate, and the sealing is finished by a silica gel pad between the movable hinge sealing cover and the integrated pressing plate; the check valve on the movable hinge sealing cover is arranged in a stepped circular hole at the movable end of the movable hinge sealing cover.
Furthermore, the integrated pressing plate is arranged above the storage device structure, a large stepped round hole at the bottom of the integrated pressing plate is matched with a stepped surface of the reagent cabin, a U-shaped groove opening arranged at the middle position of the integrated pressing plate is embedded into a clamping column on the movable hinge sealing cover when the movable hinge sealing cover is opened, and a circular hole surface is arranged below the U-shaped groove to assemble a sliding guide way; a small D-shaped hole is formed above the integrated pressing plate and used for the entrance and exit of organisms, a large D-shaped groove is formed above the integrated pressing plate and used for installing a movable hinge cover, and a round hole below the integrated pressing plate is used for being matched with a biological sample cabin; the middle layer clamping plate is arranged in the middle of the conserver structure, the biological sample cabin is clamped through the movable clamping block of the middle layer clamping plate, and a reagent pipeline inserted by a small circular through hole is arranged at the vacant position of the middle layer clamping plate; the bottom plate is arranged at the bottom of the conservator structure, so that the reagent chamber is arranged in the upper circular groove, the transition sleeve on the bottom plate is used for overlapping the magnetic pump to eliminate the length defect of the magnetic pump, and water inlets are formed in two sides of the transition sleeve; the support rod is used for connecting the limiting integrated pressing plate, the middle layer clamping plate and the bottom plate, and the driving device, the reagent cabin, the magnetic pump and the biological sample cabin are compressed and fixed by the long screw rod arranged in the support rod.
Furthermore, the anti-rotation bolt of the sealing cover comprises a movable mandrel bolt, a restoring pressure spring, a blocking embedded blocking cap and a movable cavity formed by an integrated pressing plate, wherein the restoring pressure spring is arranged at the opening end of the movable mandrel bolt and is jointly placed in the movable cavity through the blocking embedded blocking cap, one end of the movable mandrel bolt is clamped in a clamping column on the sealing cover closing and sliding mechanism when the sealing cover is closed, a pattern cushion block is arranged on the outer end face of the movable mandrel bolt and used for manually sliding the movable mandrel bolt so that the sealing cover is fixed when the sealing cover is opened, and the front end of the movable cavity is close to the mounting position of the blocking embedded blocking cap and is provided with a small water drainage hole; the integrated pressure plate is close to the moving cavity and is provided with a moving stroke concave surface; the movable mandrel bolt is connected with the magnetic pump through a pipeline, the pipeline is in smooth transition through a large R fillet, seawater output by the magnetic pump firstly flows into a movable cavity formed by the integrated pressing plate through the first water filling port, the movable mandrel bolt is pushed by water pressure, the movable mandrel bolt is moved out of a clamping column of the sealing cover closing sliding mechanism, and the moving state is kept.
Further, the reagent cabin comprises a reagent cabin, an injection pressure chamber, an upper protective cover, a lower protective cover, a reagent soft diaphragm, a movable guide pipe, a pressing plate, a split type one-way valve and a plug; the reagent soft diaphragm is fixedly connected with the upper protective cover and the lower protective cover to form a reagent chamber and an injection chamber, and U-shaped convolution attachment of the reagent soft diaphragm during movement is realized by extruding, fixing and supporting the reagent soft diaphragm at the bottom of the reagent soft diaphragm through the movable guide tube and the pressing plate; the split one-way valve is arranged on one side above the reagent chamber, is communicated outwards in a single direction and is symmetrically distributed with the liquid injection port; the reagent chamber adopts a conical surface design, and a vertical small hole is formed in the liquid injection port for discharging gas during reagent injection; the pressure injection chamber is communicated with a third water injection port of the magnetic pump through a water pressure input pipeline, seawater output by the magnetic pump is injected into the pressure injection chamber through the pressure increase of the third water injection port after the movement of the movable mandrel bolt and the movable hinge sealing cover is finished through the first water injection port and the second water injection port respectively, and the reagent in the reagent chamber is injected into the biological sample chamber through the split one-way valve by driving the reagent soft diaphragm under the action of water pressure; the reagent cabin is connected with the reagent output pipeline through a split type one-way valve and communicated with the biological sample cabin, and a ball valve is arranged in the pipeline to enable the biological sample cabin to be conveniently taken out integrally when a sample is collected after the pipeline is closed.
Furthermore, split type check valve comprises valve body, regulating block, spring, case and sealing washer, split type check valve changes fixative output flow through the position change spring pressure size of removal regulating block to the liquid speed of annotating of control biological sample cabin.
Further, the biological sample cabin comprises a transparent collection cabin body, a cabin cover, a reagent uniform distribution baffle and a biological escape prevention pouring port; the reagent uniformly-distributed baffle is arranged in a stepped groove of the hatch cover through a clamp spring, small non-uniformly-distributed holes are formed in the reagent uniformly-distributed baffle, sparse holes with non-uniform sizes are formed in the positions far away from a reagent injection port, and tight holes with non-uniform distribution are formed in the positions close to the reagent injection port; the side surface of the cabin cover is provided with a threaded hole which is connected with the through seat and communicated with the reagent output pipeline, and the cabin cover is connected and sealed with the transparent collection cabin body through threads and an O-shaped ring; the anti-biological-escape inverted opening is matched with the conical net and the conical support to be arranged at an inlet of the transparent collection cabin body to prevent biological escape, the transparent collection cabin body is tightly pressed after being matched with the integrated pressing plate, and an O-shaped ring is arranged at the matching position of the transparent collection cabin body and the integrated pressing plate to seal.
Has the advantages that:
1. the invention uses a power source, namely a magnetic pump to drive a plurality of different execution devices simultaneously, and slides and moves the mandrel bolt to open the limit movable hinge sealing cover through water pressure, at the moment, the flexible guide rod along with the change of shape drives the movable hinge sealing cover to turn over while driving the piston through the water pressure, the biological sample cabin is closed under the attraction of the magnet, and simultaneously, the water pressure output by the magnetic pump injects the reagent in the reagent cabin into the biological sample cabin; in the whole driving process, different execution devices are driven by smaller power to complete the injection of the reagent and the closing of the biological sample cabin, so that redundant power source driving is overcome, and the practicability and reliability of deep sea equipment are improved;
2. the invention provides a soft diaphragm movable reagent chamber structure, and provides a sample fixing mode for injecting a reagent into a biological sample chamber by using a hydraulic pressure extrusion reagent soft diaphragm, so that the reagent in the chamber can be completely injected into the biological sample chamber, the mixing proportion can be adjusted and fixed by the volume of the reagent, the mechanical movement fault is reduced, the reagent waste is avoided, and the use cost is reduced;
3. the invention provides a biological sample sealing structure using a movable hinge cover, and designs the biological sample cabin to be hollow without shielding, thereby not only greatly improving the trapping rate of benthonic organisms, but also being used for introducing biological samples collected by other sampling equipment, and increasing the applicability of collecting and storing deep sea biological samples;
4. the invention provides a method for effectively sealing a reagent in a reagent chamber by using a split type one-way valve, thereby overcoming the influence on organisms before reagent injection so as to avoid the influence of leakage of the reagent on the trapping of the organisms, and simultaneously controlling the injection speed of a fixing agent by adjusting the split type one-way valve so as to improve the mixing uniformity and efficiency; the invention provides a method for arranging the reagent uniformly-distributed baffle at the bottom of the biological sample cabin, which can effectively improve the uniform mixing distribution of the reagent during injection.
5. The invention provides a method for integrating the execution part of the driving device on the integrated pressing plate by using the integrated pressing plate, thereby greatly improving the integrity and high concentration of the equipment.
Drawings
FIG. 1 is a front view of the overall structure of the present invention;
FIG. 2 is a top view of the living hinge cover of the present invention closed;
FIG. 3 is a cross-sectional view of the living hinge cover of the present invention closed;
FIG. 4 is a top view of the living hinge cover of the present invention when open;
FIG. 5 is a cross-sectional view of the living hinge cover of the present invention open;
FIG. 6 is a cross-sectional view of a reagent chamber of the present invention;
FIG. 7 is a cross-sectional view of a biological specimen capsule of the present invention;
FIG. 8 is a view of the water injection port of the magnetic pump of the present invention;
FIG. 9 is a uniform reagent distribution baffle of the present invention;
fig. 10 is a broken-away view of the split check valve of the present invention.
The reference numbers in the figures are: 1-reagent chamber; 1-1-lower protective cover; 1-2-moving the guide tube; 1-3-reagent soft membrane; 1-4-pressing plate; 1-5-upper protective cover; 1-6-plug; 1-7-split one-way valve; 1-7-1-valve body; 1-7-2-adjusting block, 1-7-3-spring, 1-7-4-valve core and 1-7-5-sealing ring; 2-an integrated press plate; 3-biological sample cabin; 3-1-hatch cover; 3-2-reagent uniform distribution baffle; 3-3-a transparent collection chamber; 3-4-prevention of escape from the pouring spout; 4-middle layer splint; 5-a ball valve; 6-a bottom plate; 7-a transition sleeve; 8-a magnetic pump; 8-1-a first water injection port; 8-2-a second water injection port; 8-3-a third water injection port; 9-movable hinge cover sealing; 10-clamping column; 11-a one-way valve; 12-a support bar; 13-flexible guide rod changing with shape; 14-a sliding piston; 15-moving the mandrel bolt; 16-barrier insert stop cap; 17-restoring the pressure spring; 18-pattern cushion blocks; 19-reagent output line; 20-large R pipe; 21-a slippage guide path; 22-magnet.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the accompanying drawings and embodiments.
A hydraulically-driven deep sea biological sample holder as shown in fig. 1 to 5 and 8 includes a driving means, a reagent chamber 1, a biological sample chamber 3 and a combined frame; the driving device is specifically as follows: the magnetic pump 8 drives seawater to sequentially inject a sealing cover anti-rotation bolt, a sealing cover closing sliding mechanism and a reagent chamber 1 through a first water injection port 8-1, a second water injection port 8-2 and a third water injection port 8-3, wherein a chamber body of the magnetic pump 8 penetrates through a round hole of an intermediate layer clamping plate 4 for limiting, a water inlet end and a water outlet end are seated in a transition sleeve 7, the position of a water suction port is improved while the length direction of the magnetic pump 8 is compensated for, and the situation that sundries such as silt enter and damage connected instruments when the base is trapped is avoided;
the anti-rotation bolt of the sealing cover comprises a moving mandrel bolt 15, a restoring pressure spring 17, a blocking embedded blocking cap 16, a pattern cushion block 18 and a moving cavity formed by the integrated pressing plate 2; the anti-rotation bolt of the sealing cover is communicated with a first water filling port 8-1 of the magnetic pump 8 through a large R pipeline 20 through a through seat; the movable mandrel bolt 15 is placed in a movable cavity formed by the integrated pressing plate 2, the restoring compression spring 17 is positioned at the opening end of the movable mandrel bolt 15 and is embedded in a stepped hole of the blocking embedded blocking cap 16, the movable mandrel bolt 15 is screwed into the front end of the movable cavity through threads, and the restoring compression spring 17 is compressed and moves in the movable mandrel bolt under the action of water pressure during work; the pattern cushion block 18 is fixed at the outer end face of the movable mandrel bolt 15 through a screw so as to facilitate the sliding of the movable mandrel bolt 15 through manual operation; when the restoring pressure spring 17 is in the original position, the inserting end of the moving mandrel bolt 15 is clamped into a clamping column groove after the sealing cover closing sliding mechanism is opened, the moving mandrel bolt 15 is limited to move and rotate in a non-working state, the moving mandrel bolt 15 can move to enable the sealing cover closing sliding mechanism to be in a free state, and the flexible guide rod 13 changing along with the shape in the sealing cover closing sliding mechanism pushes the movable hinge sealing cover 9 to rotate under the hydraulic action of the driving device.
The sealing cover closing sliding mechanism comprises a sliding piston 14, a sliding guide path 21, a shape-change-following soft guide rod 13, a movable hinge sealing cover 9, a clamping column 10, a one-way valve 11 and a magnet 22; the two ends of the sliding guide path 21 are respectively matched and connected with a second water outlet 8-2 of the magnetic pump 8 and a circular groove of the integrated pressing plate 2 through O-shaped rings, and a sliding piston 14 matched with the shape-changing soft guide rod 13 is placed in a sliding hole; the movable hinge cover 9 is formed by a hinge connected by a hinge, wherein a fixed end is fixed in a large D-shaped groove through a pin shaft and a bolt and the integrated pressure plate 2, a movable end is connected with the other end of the shape-following change soft guide rod 13 in a gluing matching way through a clamping column 10, and when the movable end is completely opened, in order to be closed under the underwater without being influenced by the outside, the groove of the clamping column 10 is matched with an insertion end of a moving mandrel bolt 15 of an anti-rotation bolt of the cover, and when the movable end works, the shape-following change soft guide rod 13 connected with a sliding piston 14 is driven to rotate by the hydraulic action of a driving device, and is attracted and sealed by magnets 22 arranged in square grooves which are symmetrically distributed at the upper part and the lower part of the integrated pressure plate 2; the one-way valve 11 is arranged in a stepped circular hole at the movable end of the movable hinge sealing cover 9, so that the one-way discharge of the redundant seawater in the process of injecting the reagent into the biological sample cabin 3 after the movable hinge sealing cover 9 is closed is realized.
The reagent chamber 1 shown in FIG. 6 comprises a reagent chamber, an injection pressure chamber, a reagent soft diaphragm 1-3, a movable guide tube 1-2, a pressure plate 1-4, a split type one-way valve 1-7 and a plug 1-6; the reagent chamber and the injection chamber are fixedly connected with an upper protective cover 1-5 and a lower protective cover 1-1 through a reagent soft diaphragm 1-3 by bolts, and are extruded, fixed and supported at the bottom of the reagent soft diaphragm 1-3 through a movable guide tube 1-2 and a pressure plate 1-4 to realize U-shaped convolution attachment when the reagent soft diaphragm 1-3 moves;
the split type one-way valve 1-7 shown in figure 10 comprises a 1-7-1 valve body, a 1-7-2 adjusting block, a 1-7-3 spring, a 1-7-4 valve core and a 1-7-5 sealing ring, wherein a reagent is conducted outwards in a single direction through the 1-7-1 valve body directly arranged above an upper protective cover 1-5, the reagent is injected into a reagent chamber through a flexible pipe and injection ports symmetrically distributed with the reagent, a conical surface design is adopted for facilitating the air exhaust inner wall in the chamber, and a vertical small hole is arranged at the injection port; the injection chamber is communicated with a third water injection port of the magnetic pump 8 through a water pressure input pipeline, so that when the device works, seawater input by the magnetic pump 8 enters the injection chamber through the water pressure input pipeline, the reagent soft membranes 1-3 are extruded and driven under the action of water pressure, the movable guide pipe 1-1 is driven to move, the reagent soft membranes 1-3 are kept in a U-shaped convolution fit state, most of reagents are input into the biological sample cabin 3 through the split one-way valves 1-7 through the reagent output pipeline 19 and the ball valve 5, the ball valve 5 is in an open state at the moment, and the ball valve 5 is closed when the sample collection biological sample cabin 3 is integrally taken out.
As shown in fig. 7 and 9, the biological sample chamber 3 comprises a transparent collection chamber body 3-3, a chamber cover 3-1, a reagent uniform distribution baffle 3-2 and a biological escape prevention pouring port 3-4; the reagent uniformly-distributed baffle 3-2 is arranged in a stepped groove of the hatch cover 3-1 through a clamp spring, sparse and unevenly-distributed holes are arranged at the position far away from a reagent injection port, and tight and unevenly-distributed holes are arranged close to the reagent injection port; the cabin cover 3-1 is connected and sealed with the transparent collection cabin body 3-3 through threads and an O-shaped ring, a threaded hole on the side face of the cabin cover is communicated with a reagent output pipeline 19 through a through seat, a biological escape preventing inverted opening 3-4 consisting of a conical netting and a conical support is arranged on the step face of the transparent collection cabin body 3-3 to prevent biological escape, the transparent collection cabin body 3-3 is matched with the integrated pressure plate and then is compressed, and the O-shaped ring seal is arranged on the matching position of the transparent collection cabin body and the integrated pressure plate.
The combined frame comprises an integrated pressing plate 2, a middle layer clamping plate 4, a bottom plate 6 and a supporting rod 12; the integrated pressing plate 2 is arranged above the structure of the invention, a large step round hole at the bottom of the integrated pressing plate is matched with a step surface of the reagent cabin shown in figure 6, a clamping column 10 on the movable hinge page sealing cover is embedded into a U-shaped notch at the middle position when the movable hinge page sealing cover is opened, a sliding guide way 14 is arranged in a round hole below the U-shaped notch, a small D-shaped hole on the sliding guide way is used for the in and out of organisms, a movable hinge page sealing cover is arranged in a large D-shaped groove, and a biological sample cabin 3 is arranged in a round hole below the small D-shaped hole; the middle layer splint 4 is arranged in the middle of the structure and consists of a fixed end and a movable end, wherein the fixed end is used for limiting the radial freedom degrees of the reagent cabin, the magnetic pump 8 and the sliding guide channel 1-2, the biological sample cabin 3 is clamped by the movable end of the middle layer splint, and a small circular through hole is arranged at the vacant position of the middle layer splint for inserting a reagent pipeline; the bottom plate 6 is arranged at the bottom of the structure of the invention, so that the reagent cabin is arranged in the upper circular groove, the transition sleeve 7 on the bottom plate is used for overlapping the magnetic pump 9 to eliminate the length defect, and water inlets are arranged at two sides of the transition sleeve 7; the support rod 12 is used for connecting the spacing integrated pressure plate 2, the middle layer clamping plate 4 and the bottom plate 6, and a long screw rod arranged in the support rod compresses and fixes the driving device, the reagent cabin 1, the magnetic pump 8 and the biological sample cabin 3.
Claims (7)
1. A deep sea biological sample conservator driven by water pressure comprises a driving device, a reagent cabin (1), a biological sample cabin (3), a combined frame, a sealing cover anti-rotation bolt and a sealing cover closing sliding mechanism, wherein the combined frame comprises an integrated pressing plate (2), a middle layer clamping plate (4), a bottom plate (6) and a supporting rod (12), the driving device is arranged on the middle layer clamping plate (4) and the bottom plate (6), the biological sample cabin (3) is arranged on the integrated pressing plate (2) and the middle layer clamping plate (4), the reagent cabin (1) is communicated with the biological sample cabin (3) through a reagent output pipeline (19), the sealing cover anti-rotation bolt is communicated with the driving device through a large R pipeline (20), the sealing cover closing sliding mechanism is connected with the driving device through a smooth round inner wall hole pipe, and is characterized in that the driving device comprises a magnetic pump (8), the magnetic pump is provided with a first water injection port (8-1), a second water injection port (8-2) and a third water injection port (8-3), the magnetic pump (8) drives seawater to sequentially inject a sealing cover anti-rotation bolt, a sealing cover closing sliding mechanism and a reagent cabin (1) through the first water injection port (8-1), the second water injection port (8-2) and the third water injection port (8-3); the first water injection port (8-1) is tangent to the water outlet of the magnetic pump (8) in a straight-through manner, the second water injection port (8-2) is located at the middle vertical position of the water outlet and is upward, the caliber of the second water injection port is smaller than that of the first water injection port (8-1), the third water injection port (8-3) is close to the pump head and is downward, and the caliber of the third water injection port is smaller than that of the second water injection port (8-2).
2. The deep sea biological sample conservator driven by water pressure according to claim 1, wherein, the sealing cover closing sliding mechanism is composed of a sliding piston (14), a sliding guide path (21), a shape-changing soft guide rod (13), a movable hinge cover (9), a clamping column (10) and a magnet (22); one end of the sliding guide path (21) is connected with a second water filling port (8-2) of the magnetic pump (8) by adopting a smooth round inner wall hole pipe, the other end of the sliding guide path is matched and connected with a round groove of the integrated pressure plate (2) through an O-shaped ring, and the sliding piston (14) is arranged at one end of the shape-following flexible guide rod (13); the movable hinge sealing cover (9) is hinged with the integrated pressing plate (2) through a connecting rod, a clamping column (10), a magnet (22) and a one-way valve (11) are arranged on the movable hinge sealing cover (9), the clamping column (10) is connected with the other end of the shape-following change soft guide rod (13), the clamping column (10) is provided with a groove, an opening faces to the sealing cover anti-rotation bolt during assembly, after the movable hinge sealing cover (9) is completely opened, a movable mandrel bolt (15) of the sealing cover anti-rotation bolt is inserted into the groove of the clamping column (10), seawater output by the magnetic pump (8) drives a sliding piston (14) through a second water injection port (8-2), and the connected shape-following change soft guide rod (13) drives the movable hinge sealing cover (9) to turn over, so that the biological sample cabin (3) is closed; the magnets (22) are respectively embedded in the grooves of the movable hinge cover (9) and the integrated pressing plate (2), and the magnets (22) are provided with a plurality of pieces which are distributed in an opposite manner in an up-down symmetrical manner; the total length of the shape-following-change soft guide rod (13) and the sliding piston (14) after being matched is not more than 3/4 of the sliding guide path (21), the diameter ratio of the diameter of the sliding piston (14) to the inner diameter of the sliding guide path (21) is less than 5/6, a matching gap is formed, the conservator drives the sliding piston (14) to drive the movable hinge sealing cover (9) connected with the shape-following-change soft guide rod (13) under small water pressure, the movable hinge sealing cover (9) is turned over, the magnet (22) on the movable hinge sealing cover (9) is attracted with the magnet (22) on the integrated pressing plate (2) to seal the biological sample cabin, and sealing is completed through a silica gel pad between the movable hinge sealing cover (9) and the integrated pressing plate (2); the check valve (11) on the movable hinge cover (9) is arranged in a stepped circular hole at the movable end of the movable hinge cover (9).
3. The deep sea biological sample conservator driven by water pressure according to claim 1, wherein the integrated pressing plate (2) is arranged above the storage structure, a large step round hole at the bottom of the integrated pressing plate (2) is matched with a step surface of the reagent cabin (1), a U-shaped groove arranged at the middle position of the integrated pressing plate (2) is embedded into a clamping column (10) on the movable hinge cover (9) when the movable hinge cover (9) is opened, and a sliding guide way (21) is arranged on the round hole surface below the U-shaped groove; a small D-shaped hole is formed above the integrated pressing plate (2) and used for the entrance and exit of organisms, a large D-shaped groove is formed above the integrated pressing plate (2) and used for installing a movable hinge cover (9), and a round hole below the integrated pressing plate (2) is used for being matched with and installing a biological sample cabin (3); the middle layer splint (4) is arranged in the middle of the conservator structure, the biological sample cabin (3) is clamped through the movable clamping block of the middle layer splint (4), and a small round through hole is arranged at the vacant position of the middle layer splint (4) to insert a reagent output pipeline (19); the bottom plate (6) is arranged at the bottom of the conservator structure, so that the reagent chamber (1) is arranged in the circular groove above the bottom plate, the transition sleeve on the bottom plate (6) is used for superposing the magnetic pump (8) to eliminate the length defect of the magnetic pump, and water inlets are formed in two sides of the transition sleeve; the support rod (12) is used for connecting the limiting integrated pressing plate (2), the middle clamping plate (4) and the bottom plate (6), and the long screw rod arranged in the support rod (12) is used for compressing and fixing the driving device, the reagent cabin (1), the magnetic pump (8) and the biological sample cabin (3).
4. The deep sea biological sample conservator driven by water pressure according to claim 1, wherein, the cover anti-rotation bolt comprises a moving mandrel bolt (15), a restoring pressure spring (17), a blocking embedding blocking cap (16) and a moving cavity formed by an integrated pressure plate (2), the restoring pressure spring (17) is arranged at the opening end of the moving mandrel bolt (15) and is jointly placed in the moving cavity through the blocking embedding blocking cap (16), one end of the moving mandrel bolt (15) is blocked in a blocking column (10) on the closing sliding mechanism of the cover, a pattern cushion block (18) is arranged at the outer end surface of the moving mandrel bolt (15) for manually sliding the moving mandrel bolt (15) so as to fix the cover when the cover is opened, and the front end of the moving cavity is close to the mounting position of the blocking embedding blocking cap (16) and is provided with a small water drainage hole; the integrated pressure plate (2) is close to the position of the moving cavity and is provided with a moving stroke concave surface; the movable core shaft bolt (15) is connected with the magnetic pump (8) through a large R pipeline (20), the large R pipeline (20) is in smooth transition through a large R fillet, seawater output by the magnetic pump (8) firstly flows into a movable cavity formed by the integrated pressing plate (2) through the first water injection port (8-1), the movable core shaft bolt (15) is pushed through water pressure, the movable core shaft bolt (15) is made to move out of the clamping column (10) of the sealing cover closing sliding mechanism, and the movable core shaft bolt (15) is kept in a moving-out state.
5. The deep sea biological sample conservator driven by water pressure according to claim 1, wherein the reagent chamber (1) comprises reagent chamber, injection chamber, upper protective cover (1-5), lower protective cover (1-1), reagent soft diaphragm (1-3), mobile guide tube (1-2), pressure plate (1-4), split type one-way valve (1-7) and plug (1-6); the reagent soft diaphragm is fixedly connected with the lower protective cover (1-1) through the upper protective cover (1-5) to form a reagent chamber and an injection pressure chamber, and U-shaped convolution attachment of the reagent soft diaphragm (1-3) during movement is realized through extrusion, fixation and support of the movable guide tube (1-2) and the pressure plate (1-4) at the bottom of the reagent soft diaphragm (1-3); the split one-way valves (1-7) are arranged on one side above the reagent chamber, are communicated outwards in a single direction and are symmetrically distributed with the liquid injection port; the reagent chamber adopts a conical surface design, and a vertical small hole is formed in the liquid injection port for discharging gas during reagent injection; the pressure injection chamber is communicated with a third water injection port (8-3) of the magnetic pump (8) through a water pressure input pipeline, seawater output by the magnetic pump (8) is injected into the pressure injection chamber after the movement of a movable mandrel bolt (15) and a movable hinge page sealing cover (9) is respectively completed through the first water injection port and the second water injection port, and finally the pressure of the third water injection port (8-3) is increased, and a reagent in the reagent chamber (1) is injected into the biological sample chamber (3) through a split type one-way valve (1-7) by driving a reagent soft diaphragm (1-3) under the action of water pressure; the reagent cabin (1) is connected with a reagent output pipeline (19) and communicated with the biological sample cabin (3) through split type one-way valves (1-7), and a ball valve (5) is arranged in the reagent output pipeline (19) to enable the reagent output pipeline to be closed so as to facilitate the biological sample cabin (3) to be integrally taken out when samples are collected.
6. The deep sea biological sample conservator driven by water pressure according to claim 5, wherein the split one-way valve (1-7) is composed of a valve body (1-7-1), an adjusting block (1-7-2), a spring (1-7-3), a valve core (1-7-4) and a sealing ring (1-7-5), the split one-way valve (1-7) changes the output flow of the fixative by changing the pressure of the spring (1-7-3) by moving the position of the adjusting block (1-7-2), thereby controlling the filling speed of the biological sample chamber.
7. The deep sea biological sample conservator driven by water pressure according to claim 1, wherein the biological sample chamber (3) comprises transparent collection chamber body (3-3), chamber cover (3-1), reagent uniform distribution baffle (3-2) and anti-biological escape pouring port (3-4); the reagent uniformly-distributed baffle (3-2) is arranged in a stepped groove of the hatch cover (3-1) through a clamp spring, small non-uniformly-distributed holes are formed in the reagent uniformly-distributed baffle (3-2), sparse and non-uniformly-distributed holes are formed at positions far away from a reagent injection port, and tight and non-uniformly-distributed holes are formed at positions close to the reagent injection port; the side surface of the cabin cover (3-1) is provided with a threaded hole which is connected with a through seat and communicated with a reagent output pipeline (19), and the cabin cover is connected and sealed with the transparent collection cabin body (3-3) through threads and an O-shaped ring; the anti-biological-escape pouring opening (3-4) is formed in the inlet of the transparent collection cabin body (3-3) in a matching mode through a conical netting and a conical support to prevent biological escape, when the transparent collection cabin body (3-3) is matched with the integrated pressing plate (2) and then is compressed, and an O-shaped ring is arranged at the matching position of the transparent collection cabin body (3-3) and the integrated pressing plate (2) to seal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010386255.1A CN111528191B (en) | 2020-05-09 | 2020-05-09 | Hydraulic-driven deep-sea biological sample conservator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010386255.1A CN111528191B (en) | 2020-05-09 | 2020-05-09 | Hydraulic-driven deep-sea biological sample conservator |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111528191A CN111528191A (en) | 2020-08-14 |
| CN111528191B true CN111528191B (en) | 2021-08-17 |
Family
ID=71970801
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010386255.1A Active CN111528191B (en) | 2020-05-09 | 2020-05-09 | Hydraulic-driven deep-sea biological sample conservator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111528191B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112314541B (en) * | 2020-10-21 | 2022-03-18 | 杭州电子科技大学 | Portable deep sea biological light trapping device based on cam drive |
| CN113532906B (en) * | 2021-07-08 | 2022-07-26 | 上海交通大学 | Sampler is fixed to deposit normal position |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999012475A1 (en) * | 1997-09-11 | 1999-03-18 | Biopsytec Gmbh | Method and device for withdrawing biological samples |
| CN105420097A (en) * | 2015-12-15 | 2016-03-23 | 中国科学院深海科学与工程研究所 | In-situ automatic gathering and fixing device and method applied to microorganisms at all sea depths |
| CN108142384A (en) * | 2017-12-28 | 2018-06-12 | 国家海洋局第二海洋研究所 | A kind of deep sea in-situ biological fixation trapper |
-
2020
- 2020-05-09 CN CN202010386255.1A patent/CN111528191B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999012475A1 (en) * | 1997-09-11 | 1999-03-18 | Biopsytec Gmbh | Method and device for withdrawing biological samples |
| CN105420097A (en) * | 2015-12-15 | 2016-03-23 | 中国科学院深海科学与工程研究所 | In-situ automatic gathering and fixing device and method applied to microorganisms at all sea depths |
| CN108142384A (en) * | 2017-12-28 | 2018-06-12 | 国家海洋局第二海洋研究所 | A kind of deep sea in-situ biological fixation trapper |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111528191A (en) | 2020-08-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111528191B (en) | Hydraulic-driven deep-sea biological sample conservator | |
| CN208239142U (en) | A kind of portable intelligent measurement sampling apparatus | |
| RU2415253C1 (en) | Immersed pump with cleaned in well filter | |
| CN209559571U (en) | A kind of water project management water sampler | |
| CN113640038B (en) | Pressure maintaining transfer device and method for covering water in submarine sediment | |
| CN210005299U (en) | Water sample collection system for underwater robots | |
| CN114367144A (en) | In-situ multi-channel water enrichment, filtration and fixation device and method | |
| CN107144514B (en) | Device for radial penetration experiment of cylindrical hollow soil sample and using method | |
| LU502451B1 (en) | Deep-sea biological sample preserver driven by water pressure | |
| US20230273097A1 (en) | Full-sea depth multi-point in-situ sampler, and sampling and transfer method using the same | |
| CN117108277A (en) | Wellhead full liquid sampling device | |
| CN116678678A (en) | Deep sea multifunctional double-cavity time sequence sampler and working method thereof | |
| CN115307978A (en) | Sampling repair equipment and sampling repair method for water quality and sludge | |
| CN115683978A (en) | Spontaneous imbibition experimental measurement device capable of accurately simulating reservoir and field development conditions | |
| CN211477652U (en) | Pressure-maintaining sampling device for whole-sea deep macrobiology | |
| CN112729947B (en) | Series-type deepwater automatic depth-setting water quality sample collector | |
| CN109724877A (en) | A kind of system and method for oil pipe drag-reduction effect and pressure test | |
| CN219038525U (en) | Stratified sampling device for water body monitoring | |
| CN210071392U (en) | ROV-based deep sea multichannel in-situ fluid sampling and filtering device | |
| KR102011372B1 (en) | Multi Channel Vacuum Water Sampler for In-situ Underwater Lab | |
| CN117405455A (en) | Submarine shallow surface sediment pore water in-situ experiment sample collection device | |
| CN213313635U (en) | Water sample filter equipment for environmental monitoring | |
| CN117515264A (en) | Seawater farm water inlet and outlet valve and use method thereof | |
| CN218349850U (en) | Sampling device for water quality testing | |
| CN215293831U (en) | Flat gate valve for oil exploitation device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20221114 Address after: Room 13460, Building 5, Chengzhi Business Center, No. 5000, Jiangdong 1st Road, Qiantang District, Hangzhou, Zhejiang, 310000 Patentee after: Hangzhou Huanying Technology Co.,Ltd. Address before: 310018 No. 2 street, Xiasha Higher Education Zone, Hangzhou, Zhejiang Patentee before: HANGZHOU DIANZI University |