CN111505259A - Method and system for simulating virus inactivation test in high-speed train passenger room - Google Patents
Method and system for simulating virus inactivation test in high-speed train passenger room Download PDFInfo
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- CN111505259A CN111505259A CN202010316660.6A CN202010316660A CN111505259A CN 111505259 A CN111505259 A CN 111505259A CN 202010316660 A CN202010316660 A CN 202010316660A CN 111505259 A CN111505259 A CN 111505259A
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Abstract
The invention discloses a method and a system for simulating virus inactivation test in a high-speed train passenger room, wherein the method for simulating virus inactivation test in the high-speed train passenger room comprises the following steps: preparing a target phage suspension and diluting the target phage suspension into aerosol generating solution; taking the aerosol generating liquid to generate phage aerosol in the guest room; sampling air in the passenger room to obtain the total number of bacterial colonies of the target phage in unit volume S1; filtering and sterilizing the air in the passenger room; sampling the filtered and sterilized air to obtain the total bacterial colony number S2 of the target phage in unit volume; and obtaining the deactivation rate rho. The invention carries out virus inactivation sampling on the filtered and sterilized air in the passenger room of the high-speed train by a test method, thereby evaluating the virus inactivation effect, providing scientific guidance for the research of the air purification control strategy in the passenger room of the train under the environment with virus micelles, reducing the risk of the passengers being infected by high-risk viruses and improving the public health safety level of the environment in the passenger room of the high-speed train.
Description
Technical Field
The invention particularly relates to a method and a system for simulating virus inactivation test in a high-speed train passenger room.
Background
At present, the novel coronavirus seriously harms the life safety of human beings, the virus has extremely strong infectivity, and the transmission route of the virus is mainly transmitted through substances such as aerosol with the virus, particulate matters and the like in the air, so that the virus inactivation in a closed space is an important measure for effectively inhibiting the transmission of the virus and protecting the personal health safety of passengers.
Nowadays, with the continuous development of railway technology, high-speed rails have become a main trip mode of people. Since a high-speed train is a relatively closed space, once a virus carrier exists on the train, the health of other passengers is possibly endangered. The inactivation and killing of the virus in the train carriage can be influenced to a certain extent by the passenger seating position in the high-speed train carriage and the running speed of the high-speed train, so that special systematic simulation virus inactivation test research needs to be carried out aiming at the specificity of the high-speed train.
On the premise of ensuring that the virus inactivation test achieves the expected effect, a virus inactivation test method and a virus inactivation test system considering passenger safety are required to be researched so as to improve the public health safety level of the passenger room of the high-speed train.
Disclosure of Invention
The invention aims to provide a method and a system for simulating virus inactivation test in a high-speed train passenger room, aiming at the blank of the research on the simulation virus inactivation test in the high-speed train passenger room in the prior art.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a test method for simulating virus inactivation in a high-speed train passenger room is characterized by comprising the following steps:
step 1, preparing a target phage suspension, and diluting the target phage suspension into an aerosol generating solution;
step 2, taking aerosol generating liquid with the volume of V to generate phage aerosol in a passenger room of a high-speed train;
step 3, sampling air in the passenger room of the high-speed train to obtain the total number S1 of bacterial colonies of the target phage in unit volume;
step 4, filtering and sterilizing air in the high-speed train passenger room;
step 5, sampling the air in the passenger room of the high-speed train processed in the step 4 to obtain the total number S2 of the bacterial colonies of the target phage in unit volume;
and 6, solving the deactivation rate rho by using the following formula:
preferably, in step 3, a natural sedimentation method is used for sampling.
In a preferable mode, in the step 5, the sampling is performed by using a plate sedimentation method.
As a preferable mode, the high-speed train passenger room is set according to the following conditions that the train 1 is actually operated: 1, the interior decoration of the laboratory real vehicle is the same as that of an actual running train.
Preferably, the target phage is a Serratia marcescens phage.
In a preferable mode, in the step 4, the filtering process includes filtering PM2.5 dust and germs adhered to the dust.
In a preferable mode, in the step 4, the sterilization process includes killing planktonic bacteria, removing gaseous organic substances and benzene series.
Preferably, the steps 1 to 6 are repeated a plurality of times, and the deactivation rate is averaged.
Based on the same inventive concept, the invention also provides a simulated virus inactivation test system in the high-speed train passenger room, which is characterized by comprising the following steps:
target phage suspension preparation unit: for preparing a suspension of target phage;
an aerosol-generating liquid preparation unit: the device is used for diluting the target phage suspension into aerosol generating liquid;
phage aerosol generating unit: the device is used for generating phage aerosol by using aerosol generating liquid with volume V in a passenger room of a high-speed train;
a first sampling unit: the system is used for sampling air in a passenger room of the high-speed train after the phage aerosol generating unit generates the phage aerosol, and obtaining the total colony number S1 of the target phage in unit volume;
an air purification unit: the air purifier is used for filtering and sterilizing air in the passenger room of the high-speed train;
a second sampling unit: the air purification unit is used for sampling air in the passenger room of the high-speed train after the filtering and sterilization work of the air purification unit is finished, and obtaining the total bacterial colony number S2 of the target phage in unit volume;
a calculation unit: for finding the deactivation rate p according to the following equation:
the invention carries out virus inactivation sampling on the air which is filtered and sterilized by the air purification unit in the high-speed train passenger room by a test method, thereby evaluating the virus inactivation effect, providing scientific guidance for the research of the air purification control strategy in the train passenger room under the environment with virus micelles, reducing the risk of the passengers being infected by high-risk viruses and improving the public health safety level of the environment in the high-speed train passenger room.
Detailed Description
The method is completed through a laboratory real-time test, air in a passenger room of the high-speed train is filtered and sterilized through the air purification unit through the laboratory real-time test, and then the effect of the air purification unit for inactivating the artificially polluted phage is evaluated by adopting a plate sedimentation method.
Taking a Chinese standard motor train unit with the model number of CR400AF as an example, a laboratory real-vehicle test is carried out.
The invention relates to a test method for simulating virus inactivation in a high-speed train passenger room, which comprises the following steps:
step 1, preparing a target phage SM702 suspension, and diluting the target phage suspension into an aerosol generating solution.
The preparation process of the target phage suspension comprises the following steps: taking a pure culture strain of the serratia marcescens to be cultured in a meat soup culture solution to obtain a host bacterium suspension, inoculating the host bacterium suspension and a bacterial strain bacteriophage suspension in an aseptic bottle filled with nutrient broth, and culturing overnight to carry out bacteriophage culture.
The aerosol-generating liquid is prepared by diluting phage suspension to a certain titer (such as 1.8 × 10) with common nutrient broth5pfu/ml), i.e. can be used to generate an aerosol.
And 2, adding the aerosol generating liquid with the volume of V (such as 10ml) into a glass generator, weighing, and generating phage aerosol in a passenger room of a high-speed train.
And 3, sampling the unsterilized air in the passenger room of the high-speed train by using a natural sedimentation method, and settling for 30 minutes to obtain the total bacterial colony number S1 of the target phage in unit volume.
And 4, filtering and sterilizing the air in the passenger room of the high-speed train.
The filtering process includes filtering the PM2.5 dust and pathogens adhering to the dust.
The sterilization process comprises the steps of killing planktonic bacteria, removing gaseous organic substances (TVOC), benzene series substances, peculiar smell and the like.
The filtered and sterilized fresh and clean air is supplied to the vehicle compartment.
And 5, sampling the air in the high-speed train passenger room processed in the step 4 by using a plate sedimentation method, adding a nutrient agar culture medium mixed with host bacteria into the upper layer of a sampling plate to prepare a double-layer plate, and performing phage plaque counting culture to obtain the total number of bacterial colonies of the target phage in unit volume S2.
And 6, calculating the inactivation effect to obtain the virus inactivation effect of the test, wherein the specific calculation method comprises the following steps:
the deactivation rate ρ (%):
the passenger room of the high-speed train used in the test of the invention is as follows: 1, the interior decoration of the laboratory real vehicle is the same as that of an actual running train, and is not a simulated or model train carriage.
In this embodiment, the target phage is a serratia marcescens phage, that is, the serratia marcescens phage is used as a test indicator virus.
Preferably, steps 1 to 6 are repeated a plurality of times (3 times in the present embodiment), and the inactivation rates are averaged.
The invention also provides a high-speed train passenger room virus inactivation simulation test system, which comprises:
target phage suspension preparation unit: for preparing a suspension of target phage;
an aerosol-generating liquid preparation unit: the device is used for diluting the target phage suspension into aerosol generating liquid;
phage aerosol generating unit (e.g. glass aerosol generator): the device is used for generating phage aerosol by using aerosol generating liquid with volume V in a passenger room of a high-speed train;
a first sampling unit: the system is used for sampling air in a passenger room of the high-speed train after the phage aerosol generating unit generates the phage aerosol, and obtaining the total colony number S1 of the target phage in unit volume;
an air purification unit: the air filter is used for filtering and sterilizing air in the passenger room of the high-speed train. The air purification unit is a high-energy ion air purifier or a plasma sterilizing device.
A second sampling unit: the air purification unit is used for sampling air in the passenger room of the high-speed train after the filtering and sterilization work of the air purification unit is finished, and obtaining the total bacterial colony number S2 of the target phage in unit volume;
a calculation unit: for finding the deactivation rate p according to the following equation:
while embodiments of the present invention have been described, the present invention is not limited to the above-described embodiments, which are intended to be illustrative rather than limiting, and many modifications may be made by those skilled in the art without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (9)
1. A method for simulating virus inactivation test in a high-speed train passenger room is characterized by comprising the following steps:
step 1, preparing a target phage suspension, and diluting the target phage suspension into an aerosol generating solution;
step 2, taking aerosol generating liquid with the volume of V to generate phage aerosol in a passenger room of a high-speed train;
step 3, sampling air in the passenger room of the high-speed train to obtain the total number S1 of bacterial colonies of the target phage in unit volume;
step 4, filtering and sterilizing air in the high-speed train passenger room;
step 5, sampling the air in the passenger room of the high-speed train processed in the step 4 to obtain the total number S2 of the bacterial colonies of the target phage in unit volume;
and 6, solving the deactivation rate rho by using the following formula:
2. the method for testing the inactivation of the virus in the high-speed passenger train room according to claim 1, wherein in the step 3, the sampling is performed by a natural sedimentation method.
3. The method for testing the simulated viral inactivation in the high-speed passenger train room according to claim 1, wherein in the step 5, the sampling is performed by a plate sedimentation method.
4. The method for simulating virus inactivation test in a high-speed train passenger room according to claim 1, wherein the high-speed train passenger room is a passenger room that is operated according to a ratio of: 1, the interior decoration of the laboratory real vehicle is the same as that of an actual running train.
5. The method for testing the simulated viral inactivation in the high-speed train passenger compartment of claim 1, wherein the target phage is a Serratia marcescens phage.
6. The method for simulating virus inactivation test in a high-speed train passenger compartment as claimed in claim 1, wherein in the step 4, the filtering process comprises filtering PM2.5 dust and germs adhered to the dust.
7. The method for simulating virus inactivation test in the high-speed train passenger room as claimed in claim 1, wherein in the step 4, the sterilization process comprises killing planktonic bacteria, removing gaseous organic substances and benzene series.
8. The method for testing the inactivation of a virus in a high-speed train passenger compartment according to claim 1, wherein the steps 1 to 6 are repeated a plurality of times, and the inactivation rates are averaged.
9. The utility model provides a simulated virus inactivation test system in high speed train guest room which characterized in that includes:
target phage suspension preparation unit: for preparing a suspension of target phage;
an aerosol-generating liquid preparation unit: the device is used for diluting the target phage suspension into aerosol generating liquid;
phage aerosol generating unit: the device is used for generating phage aerosol by using aerosol generating liquid with volume V in a passenger room of a high-speed train;
a first sampling unit: the system is used for sampling air in a passenger room of the high-speed train after the phage aerosol generating unit generates the phage aerosol, and obtaining the total colony number S1 of the target phage in unit volume;
an air purification unit: the air purifier is used for filtering and sterilizing air in the passenger room of the high-speed train;
a second sampling unit: the air purification unit is used for sampling air in the passenger room of the high-speed train after the filtering and sterilization work of the air purification unit is finished, and obtaining the total bacterial colony number S2 of the target phage in unit volume;
a calculation unit: for finding the deactivation rate p according to the following equation:
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010316660.6A CN111505259A (en) | 2020-04-21 | 2020-04-21 | Method and system for simulating virus inactivation test in high-speed train passenger room |
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| CN202010316660.6A CN111505259A (en) | 2020-04-21 | 2020-04-21 | Method and system for simulating virus inactivation test in high-speed train passenger room |
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| CN111505259A true CN111505259A (en) | 2020-08-07 |
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| CN202010316660.6A Pending CN111505259A (en) | 2020-04-21 | 2020-04-21 | Method and system for simulating virus inactivation test in high-speed train passenger room |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113504339A (en) * | 2021-06-02 | 2021-10-15 | 湖南中净生物科技有限公司 | Method for testing smoke inactivation effect of tertiary virus |
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| CN1472331A (en) * | 2003-06-19 | 2004-02-04 | 上海交通大学 | Method for testing virus removing effect with air purifier |
| CN101298625A (en) * | 2008-07-02 | 2008-11-05 | 中国人民解放军军事医学科学院微生物流行病研究所 | Highly effective particle filter biological detection method and positive-negative pressure facility detection system |
| CN101590798A (en) * | 2009-07-08 | 2009-12-02 | 吴世禄 | A kind of vehicle with epidemic prevention function |
| CN101603069A (en) * | 2008-06-10 | 2009-12-16 | 于玺华 | The detection method of collecting one-step virus aerosol and concentration thereof |
| CN110734951A (en) * | 2019-09-05 | 2020-01-31 | 武汉东湖科创中试基地科技有限公司 | Method for detecting virus removal rate of air purifier |
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2020
- 2020-04-21 CN CN202010316660.6A patent/CN111505259A/en active Pending
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| CN1472331A (en) * | 2003-06-19 | 2004-02-04 | 上海交通大学 | Method for testing virus removing effect with air purifier |
| CN101603069A (en) * | 2008-06-10 | 2009-12-16 | 于玺华 | The detection method of collecting one-step virus aerosol and concentration thereof |
| CN101298625A (en) * | 2008-07-02 | 2008-11-05 | 中国人民解放军军事医学科学院微生物流行病研究所 | Highly effective particle filter biological detection method and positive-negative pressure facility detection system |
| CN101590798A (en) * | 2009-07-08 | 2009-12-02 | 吴世禄 | A kind of vehicle with epidemic prevention function |
| CN110734951A (en) * | 2019-09-05 | 2020-01-31 | 武汉东湖科创中试基地科技有限公司 | Method for detecting virus removal rate of air purifier |
Non-Patent Citations (1)
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Cited By (1)
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| CN113504339A (en) * | 2021-06-02 | 2021-10-15 | 湖南中净生物科技有限公司 | Method for testing smoke inactivation effect of tertiary virus |
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Application publication date: 20200807 |