CN101298625A - Highly effective particle filter biological detection method and positive-negative pressure facility detection system - Google Patents
Highly effective particle filter biological detection method and positive-negative pressure facility detection system Download PDFInfo
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Abstract
The invention relates to a high efficient particle filter biological detecting method and a positive/negative pressure facility detecting system. The front end of the high efficient particle filter at an exhaust outlet of the biological safety cabinet is provided with a microorganism aerosol generator and an air microorganism biological sampler; meanwhile, the rear end of the high efficient particle filter is provided with one to four air microorganism biological samplers, a microorganism culture dish is arranged inside each sampler; when the air is delivered to a front window operating opening of the cabinet body, the analog indicating microorganism is produced artificially, the analog indicating microorganism is the bacterium and virus which do not exist in the natural air and are not harmful to human, animals and the environment; the analog indicating microorganism is collected by the culture dishes arranged inside the front air microorganism biological sampler and the rear air microorganism biological sampler of the high efficient particle filter; the counting is realized after the culture; the filter efficiency of the efficient particle on the microorganism is obtained by counting the ratio of the pre-counting result to the post-counting result of the high efficient particle filter.
Description
Technical field
The present invention relates to a kind of biological detection method and proofing unit, particularly about highly effective particle filter biological detection method and positive-negative pressure facility detection system in the sending of just a kind of/negative pressure facility/exhaust system.
Background technology
In biological study and experiment, often to be applied to various malleations or negative pressure facility, raise clean room's (as clean operating room, medicament compartment) etc. of the positive laminated structure of disrupter, three grades of negative pressure, level Four Biosafety chamber and various uses as the negative pressure infection animal.The setting of above-mentioned these positive-negative pressure facility air-supplies or exhaust system, to prevent the pollution of infectious polluted air that the negative pressure structure facility produces exactly, prevent that positive laminated structure facility from entering air in the facility internal contamination facility with the bioaerosol of external environment outside atmosphere.
At present, both at home and abroad to the detection of the highly effective particle filter in the various positive-negative pressure facilities (HEPA), all be to adopt abiotic aerocolloidal method, promptly adopt physics methods such as particle scanning, photo densitometry to detect evaluation.During detection, the general monodisperse aerosol (0.3 μ m or 0.5 μ m) that uses artificial generation dioctyl phthalate (DOP) (DOP), poly-alpha olefins (PAO), polyoxyethylene glycol etc., the detection of leaking.Because bioaerosol particle and abiotic aerosol particles exist at aspects such as moiety, structure, physical propertys than big-difference, particularly the microbial aerosol that produces of laboratory be polydisperse, particle diameter is little, institute is electrically charged also different because of microbe species.Therefore adopt existing method the negative pressure infection animal to be raised the detection evaluation result of highly effective particle filter in the exhaust system of disrupter, often be not inconsistent with the actual filtration effect of highly effective particle filter to microbial aerosol.Simultaneously conventional detection only to highly effective particle filter detections of lining by line scan, can't be leaked the structure of whole highly effective particle filter, damage leakage, material structure leakage make systematic detection.The physics detection method also exists the complicacy and the demanding characteristics of instrument of execute-in-place in addition.
Summary of the invention
At the problems referred to above, the purpose of this invention is to provide a kind of just being used for/negative pressure facility send/exhaust system, operational safety is reliable, detects the effect true and accurate, can not produce the highly effective particle filter biological detection method and the biological detection method of secondary pollution to environment.
For achieving the above object, the present invention takes following technical scheme: a kind of highly effective particle filter biological detection method, it is characterized in that: (1) is provided with a microbial aerosol producer and an air microorganism sampler at the filtration front end of the highly effective particle filter of a malleation or negative pressure facility, simultaneously 1～4 air microorganism sampler is set, is provided with the microorganism culturing ware in each described sampling thief in the rear end of highly effective particle filter; (2) in by the air-supply of highly effective particle filter front end, manually simulate indicator microoraganism by the air microbe aerosol dispenser to safe and efficient particle filter front end, described simulation indicator microoraganism is respectively not to be had in the natural air, to the bacterium and the virus of people, animal, the harm of environment lifeless matter; (3), under the situation of bacterium and two kinds of simulations of virus indicator microoraganism, carry out the air collection respectively by being arranged on the forward and backward air microorganism sampler of highly effective particle filter; (4) culture dish in described each collector of taking-up carries out biology to described simulation indicator microoraganism and cultivates the back counting; (5) by calculating the proportionlity that highly effective particle filter filters forward and backward count results, obtain the filtration efficiency of highly effective particle filter for microorganism.
The bacterium of described simulation indicator microoraganism is used serratia marcescens, the virus of described simulation indicator microoraganism is used the phage SM701 of serratia marcescens, the particle diameter scope of described artificial generation simulation microbial aerosol is between 0.5～3 μ m, and wherein the microbial aerosol population of 1 μ m accounts for more than 75%.
Described microbial aerosol producer is gas blowout, downdraught type producer, the aerosolized serratia marcescens liquid 〉=0.2ml of per minute under its nominal operation flow, the following sub-ratio of bioplast 〉=80% of particle diameter 2 μ m in the serratia marcescens aerosol that discharges, the release rate of microbial aerosol is 30 ± 3m/min.
The air microorganism sampler of described highly effective particle filter front end adopts six grades of air microorganism samplers of Anderson that agar plate has been installed, and be in normally at described Biohazard Safety Equipment, under the steady operational status, gather air background sample, sampling time is 10min, and the sampling thief flow is 28.3L/min.
The sampling thief of described highly effective particle filter rear end is three Anderson secondary air microorganism samplers that agar plate has been installed, each described secondary air microorganism sampler is arranged on place, exhaust outlet axis, left side, axis and the right side, axis at distance 40～60cm place, described highly effective particle filter rear end, and after simulation indicator microoraganism aerosol 10min takes place, begin to detect sampling, sampling time is 10min, the sampling thief flow is 28.3L/min, duplicate detection 3 times.
A kind of positive-negative pressure facility detection system of realizing aforesaid method, it is characterized in that: it comprises that one is arranged on the sealing body of wall in the facility, described sealing body of wall is provided with one or more highly effective particle filter, the inlet side of described highly effective particle filter or air side are negative pressure or malleation Biosafety chamber, the air side of described highly effective particle filter is connected with exhaust duct, be provided with at least one microbial aerosol producer and at least one air microorganism sampler in the inlet side of described highly effective particle filter, be provided with 1～4 air microorganism sampler in the air side of described highly effective particle filter, be provided with the microorganism culturing ware in each described sampling thief, and the outlet of each described air microorganism sampler connects an off-gas pump by a pipeline respectively.
The sampling thief of described highly effective particle filter front end is six grades of air microorganism samplers of Anderson.
The sampling thief of described highly effective particle filter rear end is three Anderson secondary air microorganism samplers, and described sampling thief is arranged on place, exhaust outlet axis, left side, axis and the right side, axis at the described highly effective particle filter exhaust outlet 40～60cm of distance place.
The present invention is owing to take above technical scheme, it has the following advantages: 1, the inventive method directly at the highly effective particle filter in malleation or air-supply of negative pressure facility or the exhaust system, has proposed a kind of novel method that adopts microbial aerosol the filtration, purification effect of highly effective particle filter to be carried out Biological Detection.2, the selected simulation indicator microoraganism of using of the microbial aerosol of employing of the present invention, be bacterium and the virus (comprising phage) that does not have in the air, and this bacterium all is safe from harm to crowd, animal, environment etc. with virus, therefore, testing process is safe and reliable, detected result true and accurate, specificity are very high.3, the present invention selects serratia marcescens to substitute malignant bacteria, select the phage of serratia marcescens to substitute Causative virus, collect the aerosol particles of highly effective particle filter front and back by the nutrient agar that is arranged in the collector simultaneously, and by carrying out serratia marcescens bacterium colony and phage plaque counting after the bacterium that falls is cultivated, and then by calculating the actual filtration effect of highly effective particle filter, realize biological detection method of the present invention, remedied the defective of existing physics detection method.4, the inventive method systematicness is strong, and whether have leakage, can also detect the framework of installing between highly effective particle filter and various positive-negative pressure facility body of wall, the pipeline and whether reach airtight sealing if not only can detect highly effective particle filter.5, realize apparatus of the present invention of the inventive method, practical simplicity not only, real result is accurate, and safe and reliable.Apparatus of the present invention can be widely used in the highly effective particle filter biological testing process under the various positive-negative pressure facility air supplying and exhausting system conditions.
Description of drawings
Fig. 1 is a negative pressure facility detection system synoptic diagram of realizing the inventive method
Fig. 2 is a malleation facility detection system synoptic diagram of realizing the inventive method
Embodiment
Below in conjunction with drawings and Examples, the present invention is described in detail.
Embodiment 1
As shown in Figure 1, present embodiment is a negative pressure facility detection system, comprise that one is arranged on the sealing body of wall 1 in the facility, sealing body of wall 1 is provided with one or more highly effective particle filter 2, the inlet side of highly effective particle filter 2 is negative pressure Biosafety chambers 3, and the air side of highly effective particle filter 2 is connected with exhaust duct 4.In the negative pressure safety cage, be provided with at least one microbial aerosol producer 5 and at least one air microorganism sampler 6, be outside equipped with 1～4 air microorganism sampler 7 in negative pressure Biosafety chamber 3, every air microorganism sampler 7 extend in the exhaust duct 4 by a sampling tube respectively.The outlet of each sampling thief 6,7 is connected an off-gas pump (not shown) by a pipeline respectively, inlet end at microbial aerosol producer 7 is identical with ordinary skill, connects a under meter, pressure warning unit, oil-filtering apparatus and an air compressor (not shown) successively by pipeline.
The artificial alternative abiotic particle of simulation indicator microoraganism that takes place of microbial aerosol producer 5 usefulness among the present invention.Wherein simulate indicator microoraganism and comprise two kinds, a kind of is bacterium, and with what do not have in the natural air, to the bacterium of people, animal, the harm of environment lifeless matter, another kind is a virus, with what do not have in the natural air, to the virus of people, animal, the harm of environment lifeless matter.Bacterium of the present invention is used serratia marcescens, also can use other non-pathogenic bacteria; Virus of the present invention is used the phage of serratia marcescens, also can use other nonpathogenic virus, phage.The present invention is carrying out in the process of Biological Detection highly effective particle filter 2, be manually to contain the simulation indicator microoraganism aerosol of bacterium respectively and contain viral simulation indicator microoraganism aerosol by microbial aerosol producer 5, and collect by being arranged on sealing body of wall 1 inside and outside sampling thief 6,7 respectively, again the bacterium or the virus of collecting are carried out biology cultivation and counting, by calculating the proportionlity that highly effective particle filter 2 filters forward and backward count results, obtain the true filtration efficiency of highly effective particle filter 2 corresponding microorganisms then.
Below by specific embodiment, the present invention is further illustrated.
The material that the present invention uses in carrying out the Biological Detection process:
1, simulation indicator microoraganism:
The bacterium of present embodiment is adopted serratia marcescens (Serratia Marcescens), and the serratia marcescens bacterium colony is a rose, and dimpling is smooth moistening, neat in edge.
Virus of the present invention adopts serratia marcescens phage SM701 suspension.
The artificial particle diameter scope that the simulation microbial aerosol takes place is between 0.5～3 μ m, and wherein the microbial aerosol population of 1 μ m accounts for 75%;
2, reagent
(1) phosphate buffered saline buffer (PBS, 0.03mol/L, pH7.2)
Get disodium hydrogen phosphate,anhydrous 2.83 grams, potassium primary phosphate 1.36 grams add distilled water to 1000ml, regulate pH to 7.2～7.4, and sterilization 20min is standby under 121 ℃ of high pressure steam.
(2) plain agar substratum
Composition: peptone 10 gram extractum carnis powder 3 gram NaCl5 gram agar powders 15 grams
Add the fusion of 1000ml distilled water or deionized water Hybrid Heating, regulate pH to 7.2～7.4, sterilization 20min is standby under 121 ℃ of high pressure steam.
(3) broth medium
Composition: extractum carnis 3 gram peptones 10 gram sodium-chlor 5 grams
Add water to 1000ml, regulate pH to 7.2～7.4, sterilization 20min is standby under 121 ℃ of high pressure steam.
(4) LB substratum (Luria-Bertani substratum)
Composition: Tryptones 10 gram yeast extracts 5 gram NaCl10 grams
Add the fusion of 1000ml distilled water or deionized water Hybrid Heating, regulate pH to 7.2～7.4, sterilization 20min is standby under 121 ℃ of high pressure steam.
3, serratia marcescens suspension preparation
(1) under aseptic condition, serratia marcescens (Serratia Marcescens) bacterial classification streak inoculation is arrived plain agar substratum plate (on 90 * 15mm);
(2) cultivate 24 ± 2h down at 30 ± 0.5 ℃;
(3) under aseptic condition from characteristic bacterium colony (rose, dimpling, smooth moistening, the neat in edge) surface picking one transfering loop bacterium transfer in the test tube that 5ml meat soup liquid nutrient medium is housed;
(4) under 37 ± 0.5 ℃, shaking table (160rpm) is cultivated 24 ± 2h;
(5) preserve down at 4 ℃, the storage life is 15 days, determines bacterial concentration with the plate dilution method of counting before using, Zhi Bei bacterium liquid as stated above, and its mean concns should be 1 * 10
10～4 * 10
10/ ml;
(6) with the PBS diluent bacterium stoste can be used by after the suitable proportion dilution.
4, the preparation and the cultivation of serratia marcescens phage SM701 suspension
(1) preparation of host bacterium bacterium liquid
The serratia marcescens that to grow on the plain agar substratum is inoculated in the meat soup liquid nutrient medium, and under 37 ℃, 150rpm shaking culture 8h preserves standby down at 4 ℃.
(2) propagation of serratia marcescens phage SM701
Serratia marcescens phagocytosis body fluid 0.1ml and host bacterium liquid 0.2ml are added in the 5ml LB liquid nutrient medium, room temperature is placed 1h, behind 37 ℃ of following 50rpm shaking culture 3.5h, and the centrifugal 10min of whizzer 10000rpm, with 0.22 μ m membrane filtration, gained amplification liquid is tired and is checked and should be 10 with supernatant liquor
9～10
10Pfu/ml preserves down at 4 ℃.As the deficiency of tiring, can repeat propagation.
(3) phage is cultivated counting:
Double-deck Plating: lower floor puts into sampling thief 6,7 and carries out taking out after the phage sampling with the LB substratum 7～9ml shop ware that contains 1.5% agar, under aseptic condition, adds in upper strata semisolid medium 4～6ml of 50 ℃ and contains 0.3ml 10
9The serratia marcescens of pfu/ml is placed on the table top then and shakes up, and makes the upper strata substratum be paved with flat board, after waiting to solidify, cultivates 12～24h down at 30～36 ℃, the counting plaque.
Adopt apparatus of the present invention to carry out biological detection method, may further comprise the steps:
1, background detects
Keep negative pressure Biosafety chamber 3 to be in normal, steady operational status,, in cabinet 1, gather air background sample with six grades of air microorganism samplers 6 of Anderson that agar plate has been installed, sampling time 10min, sampling thief 6 flows are 28.3L/min.
2, should satisfy following condition to the selection of microbial aerosol producer 5:
(1) gas blowout, downdraught type producer;
(2) the aerosolized serratia marcescens liquid 〉=0.2ml of per minute under the nominal operation flow;
(3) the following sub-ratio of bioplast 〉=80% of particle diameter 2 μ m in the serratia marcescens aerosol of Shi Fanging;
(4) release rate of microbial aerosol is 30 ± 3m/min.
3, following method is adopted in 5 calibrations to the microbial aerosol producer:
(1) area (m of the fog nozzle of measurement microbial aerosol producer
2), when calculating the speed ejection aerosol with 0.5m/s, the airshed (m of aerosol dispenser
3/ s);
(2) the husky Lei Shi suspension of the cement bacterium of adding certain volume in microbial aerosol producer 5, suspension bacterium colony concentration is calculated with plate dilution method;
(3) open microbial aerosol producer 5 and sampling thief 6, the control airshed, making aerosol ejection speed is 0.5m/s, to the damage rate of microorganism less than 15%.Microbial aerosol producer 5 work back shunting phegmas accurately calculate its bacterium colony concentration with plate dilution method after the measurement volumes, sampling 20min.Accurate measurement in sampling back and record residue bacteria liquid are long-pending;
(4) result calculates
Aerosol generating capacity=(bacteria liquid amasss-the phegma volume behind bacterium liquid original volume-spraying 20min)/20;
Aerosol ejection speed=air flow rate (m
3/ s)/fog nozzle area (m
2);
The sub-ratio of the following bioplast of particle diameter 2 μ m in the aerosol=6,7 grades of plate colony numbers of Andersen formula sampling thief/plate colony number sums at different levels
The rate of recovery of serratia marcescens (PBS diluent)=phegma bacterium colony concentration/bacterium liquid original content.
When present embodiment detects, use the PBS diluted to (1～8) * 10 the serratia marcescens suspension
7/ ml, ice bath is placed, and is connected on the fluid inlet of microbial aerosol producer 5 in apparatus of the present invention, and the particle diameter scope that the mimic microbial aerosol takes place is between 0.5～3 μ m, and wherein the microbial aerosol population of 1 μ m accounts for 75%.
4, the acquisition testing of exhaust duct analog sample
Place, exhaust duct 4 axis, left side, axis and right side, axis at distance highly effective particle filter outlet 40～60cm place, one sampling tube is set respectively, the outlet side that every sampling tube connects 7, three sampling thiefs 7 of an Anderson secondary air microorganism sampler respectively off-gas pump that can be connected in parallel.
After keeping negative pressure Biosafety chamber 3 to be under normal, the steady operational status, simulation indicator microoraganism aerosol 10min takes place negative pressure Biosafety chamber 3 in, begin to detect and sample.Sampling time is 10min, and sampling thief 7 flows are 28.3L/min, and every kind (comprising bacterium and virus) detected and repeat 3 times.
5, test sample culture assays
After background gathered sample, analog detection and gather sample and place 30 ± 0.5 ℃ of incubators to cultivate 24 ± 2h, carry out serratia marcescens bacterium colony and phage plaque counting.
6, the calculating of detected result of the present invention:
(1) bacterium colony concentration is with CFU/m
3Expression, the indication bacteria concentration should be 0CFU/m in the background
3
(2) averaging analog indicator aerosol load should be greater than 10 in the negative pressure Biosafety chamber 3
4CFU/m
3Whether indicator aerosol load and highly effective particle filter 2 filtered back indicator aerosol loads and calculate before 2 pairs of aerocolloidal filterabilities of indicator of exhaust duct 4 place's highly effective particle filters should be filtered according to highly effective particle filter 2, qualifiedly should judge according to relevant national standard.
As shown in Figure 2, the difference of present embodiment and embodiment 1 is, present embodiment is a malleation facility detection system, in the air side of highly effective particle filter 2 are malleation Biosafety chambers 8, the exhaust duct 4 of highly effective particle filter 2 outlets is positioned at malleation Biosafety chamber 8, microbial aerosol producer 5 is arranged on 8 outsides, malleation Biosafety chamber, the air microorganism sampler of 8 outsides, malleation Biosafety chamber is six grades of air microorganism samplers 6, and the air microorganism sampler in the malleation Biosafety chamber 8 is a secondary air microorganism sampler 7.
It is similar to Example 1 that present embodiment carries out the biological detection process, do not repeat them here.
Claims (9)
1, a kind of highly effective particle filter biological detection method is characterized in that:
(1) the filtration front end at the highly effective particle filter of a malleation or negative pressure facility is provided with a microbial aerosol producer and an air microorganism sampler, simultaneously 1～4 air microorganism sampler is set, is provided with the microorganism culturing ware in each described sampling thief in the rear end of highly effective particle filter;
(2) in by the air-supply of highly effective particle filter front end, manually simulate indicator microoraganism by the air microbe aerosol dispenser to safe and efficient particle filter front end, described simulation indicator microoraganism is respectively not to be had in the natural air, to the bacterium and the virus of people, animal, the harm of environment lifeless matter;
(3), under the situation of bacterium and two kinds of simulations of virus indicator microoraganism, carry out the air collection respectively by being arranged on the forward and backward air microorganism sampler of highly effective particle filter;
(4) culture dish in described each collector of taking-up carries out biology to described simulation indicator microoraganism and cultivates the back counting;
(5) by calculating the proportionlity that highly effective particle filter filters forward and backward count results, obtain the filtration efficiency of highly effective particle filter for microorganism.
2, a kind of highly effective particle filter biological detection method as claimed in claim 1, it is characterized in that: the bacterium of described simulation indicator microoraganism is used serratia marcescens, the virus of described simulation indicator microoraganism is used the phage SM701 of serratia marcescens, the particle diameter scope of described artificial generation simulation microbial aerosol is between 0.5～3 μ m, and wherein the microbial aerosol population of 1 μ m accounts for more than 75%.
3, a kind of highly effective particle filter biological detection method as claimed in claim 2, it is characterized in that: described microbial aerosol producer is gas blowout, downdraught type producer, the aerosolized serratia marcescens liquid 〉=0.2ml of per minute under its nominal operation flow, the following sub-ratio of bioplast 〉=80% of particle diameter 2 μ m in the serratia marcescens aerosol that discharges, the release rate of microbial aerosol is 30 ± 3m/min.
4, as claim 1 or 2 or 3 described a kind of highly effective particle filter biological detection methods, it is characterized in that: the air microorganism sampler of described highly effective particle filter front end adopts six grades of air microorganism samplers of Anderson that agar plate has been installed, and be in normally at described Biohazard Safety Equipment, under the steady operational status, gather air background sample, sampling time is 10min, and the sampling thief flow is 28.3L/min.
5, as claim 1 or 2 or 3 described a kind of highly effective particle filter biological detection methods, it is characterized in that: the sampling thief of described highly effective particle filter rear end is three Anderson secondary air microorganism samplers that agar plate has been installed, each described secondary air microorganism sampler is arranged on the place, exhaust outlet axis at distance 40～60cm place, described highly effective particle filter rear end, left side, axis and right side, axis, and after simulation indicator microoraganism aerosol 10min takes place, begin to detect sampling, sampling time is 10min, the sampling thief flow is 28.3L/min, duplicate detection 3 times.
6, a kind of highly effective particle filter biological detection method as claimed in claim 4, it is characterized in that: the sampling thief of described highly effective particle filter rear end is three Anderson secondary air microorganism samplers that agar plate has been installed, each described secondary air microorganism sampler is arranged on the place, exhaust duct axis at distance 40～60cm place, described highly effective particle filter rear end, left side, axis and right side, axis, and after simulation indicator microoraganism aerosol 10min takes place, begin to detect sampling, sampling time is 10min, the sampling thief flow is 28.3L/min, duplicate detection 3 times.
7, a kind of positive-negative pressure facility detection system of realizing as highly effective particle filter biological detection method as described in the claim 1～6, it is characterized in that: it comprises that one is arranged on the sealing body of wall in the facility, described sealing body of wall is provided with one or more highly effective particle filter, the inlet side of described highly effective particle filter or air side are negative pressure or malleation Biosafety chamber, the air side of described highly effective particle filter is connected with exhaust duct, be provided with at least one microbial aerosol producer and at least one air microorganism sampler in the inlet side of described highly effective particle filter, be provided with 1～4 air microorganism sampler in the air side of described highly effective particle filter, be provided with the microorganism culturing ware in each described sampling thief, and the outlet of each described air microorganism sampler connects an off-gas pump by a pipeline respectively.
8, a kind of positive-negative pressure facility detection system as claimed in claim 7 is characterized in that: the sampling thief of described highly effective particle filter front end is six grades of air microorganism samplers of Anderson.
9, as claim 7 or 8 described a kind of positive-negative pressure facility detection systems, it is characterized in that: the sampling thief of described highly effective particle filter rear end is three Anderson secondary air microorganism samplers, and described sampling thief is arranged on place, exhaust outlet axis, left side, axis and the right side, axis at the described highly effective particle filter exhaust outlet 40～60cm of distance place.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105510278A (en) * | 2014-09-25 | 2016-04-20 | 中国科学院大连化学物理研究所 | Test method for particle filtering efficiency of thermal desorption liner tube |
| CN111505259A (en) * | 2020-04-21 | 2020-08-07 | 中南大学 | Method and system for simulating virus inactivation test in high-speed train passenger room |
| CN112195159A (en) * | 2020-09-29 | 2021-01-08 | 深圳市疾病预防控制中心（深圳市卫生检验中心、深圳市预防医学研究所） | Bacteriophage and application thereof |
-
2008
- 2008-07-02 CN CN 200810116063 patent/CN101298625A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105510278A (en) * | 2014-09-25 | 2016-04-20 | 中国科学院大连化学物理研究所 | Test method for particle filtering efficiency of thermal desorption liner tube |
| CN111505259A (en) * | 2020-04-21 | 2020-08-07 | 中南大学 | Method and system for simulating virus inactivation test in high-speed train passenger room |
| CN112195159A (en) * | 2020-09-29 | 2021-01-08 | 深圳市疾病预防控制中心（深圳市卫生检验中心、深圳市预防医学研究所） | Bacteriophage and application thereof |
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