CN111505007A - Non-lens holographic image collecting device for fungal spores in air - Google Patents
Non-lens holographic image collecting device for fungal spores in air Download PDFInfo
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- CN111505007A CN111505007A CN202010478674.8A CN202010478674A CN111505007A CN 111505007 A CN111505007 A CN 111505007A CN 202010478674 A CN202010478674 A CN 202010478674A CN 111505007 A CN111505007 A CN 111505007A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N21/85—Investigating moving fluids or granular solids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
A lens-free holographic image acquisition device for fungal spores in air comprises a box body and is characterized in that a lens-free holographic imaging device, a spore transmission device and a spore acquisition air channel device are arranged in the box body, wherein the spore transmission device comprises a transparent spore transmission belt, the spore acquisition air channel device comprises an air box positioned above the spore transmission belt, the bottom of the air box is provided with a blanking port, the top of the air box is provided with an acquisition air channel air inlet, the side wall of the air box is provided with an acquisition air channel air outlet, the acquisition air channel air inlet is connected with an air inlet on the box body through a pipeline, the acquisition air channel air outlet is connected with an air suction fan through a pipeline, the lens-free holographic imaging device comprises an L ED light source, a small hole and a CMOS camera, light emitted by a L ED light source is irradiated onto the spore transmission belt through the small hole, and the CMOS camera is arranged below the spore transmission belt and is opposite to the.
Description
Technical Field
The invention belongs to the technical field of intelligent agriculture and bioscience, and particularly relates to a lens-free holographic image acquisition device for fungal spores in air.
Background
With the updating and popularization of spore traps, spore traps are mostly adopted for sampling and monitoring airborne plant pathogenic fungi spores. However, the traditional spore trapping method has the defects of large workload, low efficiency, time and labor waste, manual regular replacement of slides or trapping belts and the like, and cannot automatically, real-timely and large-scale rapidly monitor the concentration condition of the aerial spores in the farmland, so that the measurement result cannot reflect the real-time and dynamic change condition of the fungal spores in the large-scale farmland.
The traditional optical microscope structurally comprises a light source, an optical lens, a light detector, a mechanical part and the like. In the imaging process, a plurality of mechanical parts are used in a matched mode, and clear images can be obtained only through adjusting focal lengths for a plurality of times. Therefore, the traditional optical microscope has a complex structure and a large volume, and is inconvenient to carry, so that the method cannot be used for observing the wheat stripe rust germ spores in a large-scale, rapid and all-weather real-time manner. Meanwhile, the field of view of the traditional optical microscope is small due to the fact that the traditional optical microscope comprises a plurality of lenses, and only a very small area of a cell to be detected can be seen at one time. In practice, professional researchers are required to observe the counts, and national satisfactory researchers are far from sufficient relative to the area to be observed. Therefore, when the spores in the air in the field are observed, the development of a device which is convenient to carry, can be used by non-professional persons for quickly collecting samples and analyzing the results in real time is urgent.
In the prior art, the invention named as a remote collecting device for microscopic images of fungal spores in air, which is filed by an inventor in thunderstorm and has the application number of 201821739143.4, the application date of 2018, 10 and 25, provides a device for automatically capturing images with spores, but the capturing device needs to be matched with a plurality of mechanical parts during working, has a complex structure and a large volume, and is inconvenient to carry; and because of the built-in precise optical microscope, the cost is expensive.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a lens-free holographic image acquisition device for fungal spores in air, which replaces the traditional microscopic imaging technology with the lens-free holographic imaging technology and creates a spore real-time detection hardware system with small volume, low manufacturing cost and good performance.
In order to achieve the purpose, the invention adopts the technical scheme that:
the utility model provides a fungi spore does not have lens holographic image collection system in air, includes box 1, its characterized in that sets up no lens holographic imaging device 2, spore transmission device 5 and spore collection air duct device 3 in box 1, wherein spore transmission device 5 includes transparent spore transmission band 13, and spore collection air duct device 3 is including being located bellows 19 of spore transmission band 13 top, and bellows 19 bottom has blanking mouth 22, and there is collection wind channel air intake 20 at the top, and the lateral wall has collection wind channel air outlet 21, and collection wind channel air intake 20 links to each other through air intake 7 on pipeline and the box 1, and collection wind channel air outlet 21 passes through the pipeline and links to each other with induced draft fan 17, no lens holographic imaging device 2 includes L ED light source 9, aperture 10 and CMOS camera 11, and the light that L ED light source 9 sent shines to spore transmission band 13 through aperture 10 on, CMOS camera 11 sets up in spore transmission band 13 below, and is just right with aperture 10.
Preferably, the back of the box body 1 is provided with an installation lug 8 with a screw hole 15, the air inlet 7 is provided with a gauze, the top cover 18 is installed at the top of the box body 1, the top cover is provided with a rain baffle, the rain baffle is fixed through support legs, and the side wall of the box body 1 is provided with a switch for controlling the storage battery and a charging port.
Preferably, the spore conveyor belt 13 is connected with two stepping motors 14, driven by the stepping motors to move left and right, and guided by the guide wheels 12.
Preferably, the invention also comprises a control device 4, the control device 4 is connected with the stepping motor 14 and the CMOS camera 11, the stepping motor 14 is controlled to transmit the collected spores to the middle of the CMOS camera 11, the CMOS camera 11 is controlled to collect lens-free holographic images, and the remote image transmission of the collecting device is realized.
Preferably, the control device 4 is connected to the CMOS camera 11 through a USB data line and a raspberry pi.
Preferably, the L ED light source 9 is a monochromatic coherent light source, and is the only light source of the whole device, and is packaged in a closed shell with only a small hole 10 at the lower part, and the light beam passing through the small hole 10 completely covers the detection area of the CMOS camera 11.
Preferably, the collection air duct of the spore collection air duct device 3 is in a right-angle form of an upper vertical section, a horizontal section and a lower vertical section from an air inlet 20 of the collection air duct to a blanking port 22 in the air box 19, and an air outlet 21 of the collection air duct is formed on a corresponding side wall of the lower vertical section.
Preferably, the distance between the blanking opening 22 and the upper surface of the spore conveying belt 13 is 1-3 mm. The upper surface of the spore transmission belt 13 is provided with an adhesive coating for adhering spores.
Preferably, a storage battery 6 is arranged at the bottom of the device, and the storage battery 6 is used for supplying power to the whole device and is arranged inside the box body 1.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention is a small platform, has light weight, small volume and convenient carrying, and can be used for on-site rapid detection.
2. The invention is based on the holographic optical principle, has simple structure and can achieve good effect without matching a plurality of mechanical parts.
3. The devices required by the invention are all general products in the market, and the manufacturing cost is low.
Drawings
FIG. 1 is a schematic view of the main structure of the apparatus of the present invention.
FIG. 2 is a schematic view of a lensless holographic imaging apparatus of the present invention.
Fig. 3 is a schematic view of an air duct device for collecting spores of the invention.
Fig. 4 is a flow chart of the operation of the acquisition device of the present invention.
Detailed Description
The following will explain the implementation mode of the invention in detail by taking the collection of the wheat stripe rust fungus summer spores and the selection of the adhesive tape as an example in combination with the attached drawings of the invention. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
As shown in FIG. 1, the device for collecting a lens-free holographic image of fungal spores in air comprises a box body 1, wherein a lens-free holographic imaging device 2, a spore transmission device 5 and a spore collection air duct device 3 are arranged in the box body 1.
Wherein, 1 back of box sets up the installation auricle 8 that has screw 15, and air intake 7 is provided with the gauze, and top cap 18 is installed at 1 top of box, and the top cap is equipped with the weather shield, and the weather shield passes through the stabilizer blade to be fixed, is equipped with the switch and the mouth that charges of control battery on the 1 lateral wall of box.
The spore transmission device 5 is used for transmitting the captured spores to a detection area of the lens-free holographic imaging device and comprises a transparent spore transmission belt 13, the spore transmission belt 13 needs to be kept horizontal, two ends of the spore transmission belt 13 are respectively connected with a stepping motor 14, the two stepping motors are driven to move left and right and are guided by a guide wheel 12, specifically, two ends of the spore transmission belt 13 can be respectively connected to a feeding wheel and a recycling wheel, and the two material wheels are respectively connected with an output shaft of the stepping motor 14.
The upper surface of the spore transfer belt 13 may be provided with an adhesive coating to which spores are adhered. The adhesive coating can be selected from transparent materials with self-adhesive property, such as adhesive tape, high transparent PC tape, etc.
As shown in fig. 2, the lens-free holographic imaging device 2 is used for performing holographic imaging on captured spores, and includes a light source assembly, a light through hole assembly, and an image collecting assembly, in this embodiment, the light source assembly includes a light source fixing structure, a voltage stabilizing circuit, and a L ED light source 9, the L ED light source 9 is the only light source of the whole device, and in order to avoid interference of other light sources, it needs to be packaged, in this embodiment, it is packaged in a closed housing with only a small hole 10 left at the lower portion, the small hole 10 is used as the light through hole assembly, the image collecting assembly is a CMOS camera 11, and is disposed below the spore transmission belt 13 and right opposite to the small hole 10, light emitted by the L ED light source 9 irradiates the spore transmission belt 13 through the small hole 10, and spores thereon are projected onto the CMOS camera 11, preferably, a light beam passing through the small hole 10 should completely cover a detection area of the CMOS camera 11.
The spore collecting air duct device 3 is used for collecting spores suspended in air and comprises an air box 19 positioned above a spore transmission belt 13, wherein the air box 19 is a hollow shell, the bottom of the air box is provided with a blanking port 22, the top of the air box is provided with a collecting air duct air inlet 20, the side wall of the air box is provided with a collecting air duct air outlet 21, and the distance between the blanking port 22 and the upper surface of the spore transmission belt 13 is about 1-3 mm. The collection air duct air inlet 20 is connected with the air inlet 7 at the top of the box body 1 through a pipeline, and the collection air duct air outlet 21 is connected with the suction fan 17 through a pipeline.
The bottom of the device is provided with a storage battery 6, the storage battery 6 supplies power for the whole device, the storage battery is arranged in the box body 1, and a rechargeable storage battery with 12V/5A direct current can be adopted.
According to the above structure, the process of acquiring images of the present invention is as follows:
the spore collection air duct device 4 starts the air suction fan 17, spores enter the collection air duct device 3 through the air inlet 7 on the box body, and are finally enriched on the spore conveying belt 13 from the blanking port 22 of the air box 19, collected spores are conveyed to the central position of the lens-free holographic imaging device 2 by controlling the stepping motor 14, the L ED light source 9 is started, light emitted by the L ED light source 9 irradiates the spore conveying belt 13 through the small hole 10, the spores on the spore conveying belt are projected to the CMOS camera 11, and the collection is completed by the CMOS camera 11.
Example 2
Referring to fig. 3, in the present embodiment, the collection air duct of the spore collection air duct device 3 is in a right-angled form of an upper vertical section-a horizontal section-a lower vertical section in the air box 19 from the air inlet 20 to the blanking port 22 of the collection air duct, and the air outlet 21 of the collection air duct is opened on the corresponding side wall of the lower vertical section. The spore transmission belt 13 can be brought into sufficient contact with the spores in the bellows 19, and the outside air flow can be isolated from the inside of the bellows 19 to avoid contaminating the inside environment.
Example 3
In this embodiment, the device further includes a control device 4 to realize automatic control, the control device 4 is connected to the stepping motor 14 and is connected to the CMOS camera 11 through the USB data line and the raspberry pi, the control device 4 controls the stepping motor 14 to transmit the collected spores to the center of the CMOS camera 11, and the CMOS camera 11 is controlled to collect lens-free holographic images through image collecting signals sent from the raspberry pi to the CMOS camera 11.
In this embodiment, the device uses the raspberry group as a control core, the lens-free holographic imaging device 2 is connected with the raspberry group through a USB line, the driving module and the I/O module are connected with the raspberry group through a communication 485 bus to realize signal transmission, the driving module adjusts the number of different pulses output by the driving chip through PWM signals, thereby realizing the movement of the stepping motor 14, the limit switch is connected with the driving module to control the motor to start and stop, and the I/O module is connected with the suction fan and the control module.
As shown in fig. 4, the working flow in this embodiment is as follows:
step S1: the raspberry group is powered on, and software is automatically started;
step S2: the system self-check is used for sending handshake commands to the driving module in sequence and detecting whether each part of device works normally or not;
step S3: the air suction fan 17 is started by the spore collecting air channel device 3, and the capturing time can be set according to the actual requirement;
step S4, after the capture is finished, the air suction fan 17 is closed, the spore conveying belt 13 moves clockwise, the spore adsorption position is horizontally conveyed to the center of the lens-free holographic imaging device 2, and the L ED light source 9 is started;
step S5: starting a CMOS camera 11 to collect a lens-free holographic image, and storing image data in a hard disk by a raspberry group;
step S6, turning off L ED light source 9 and finishing the acquisition;
step S7: the spore conveyor belt 13 moves clockwise to recover the observed spores.
This embodiment enables automatic remote acquisition.
The above description of the present invention is intended to be illustrative. Various modifications, additions and substitutions for the specific embodiments described may be made by those skilled in the art without departing from the scope of the invention as defined in the accompanying claims.
Claims (9)
1. The utility model provides a fungi spore does not have lens holographic image collection system in air, includes box (1), its characterized in that sets up no lens holographic imaging device (2), spore transmission device (5) and spore collection air duct device (3) in box (1), wherein spore transmission device (5) are including transparent spore transmission band (13), and air duct device (3) are gathered including bellows (19) that are located spore transmission band (13) top to spore, and bellows (19) bottom has blanking mouth (22), and there is collection wind channel air intake (20) at the top, and the lateral wall has collection wind channel air outlet (21), and collection wind channel air intake (20) link to each other through air intake (7) on pipeline and box (1), and collection wind channel air outlet (21) link to each other with induced draught fan (17) through the pipeline, no lens holographic imaging device (2) include L ED light source (9), aperture (10) and CMOS camera (11), and the aperture (10) that L ED light source (9) sent shine to spore transmission band (13), CMOS camera (11) set up in transmission band (13) below, and aperture (10) are just to.
2. The device for collecting lens-free holographic images of fungal spores in air as claimed in claim 1, wherein the back of the box body (1) is provided with a mounting lug (8) with a screw hole (15), the air inlet (7) is provided with a gauze, the top of the box body (1) is provided with a top cover (18), the top cover is provided with a rain shield, the rain shield is fixed by supporting legs, and the side wall of the box body (1) is provided with a switch and a charging port for controlling the storage battery.
3. The device for collecting the lens-free holographic image of the fungal spore in air as claimed in claim 1, wherein the spore conveying belt (13) is connected with two stepping motors (14) and driven by the stepping motors to move left and right, and is guided by the guide wheel (12).
4. The device for collecting lens-free holographic images of fungal spores in air as claimed in claim 3, further comprising a control device (4), wherein the control device (4) is connected with the stepping motor (14), the CMOS camera (11) and the 4G communication module, the stepping motor (14) is controlled to transmit the collected spores to the middle of the CMOS camera (11), the CMOS camera (11) is controlled to collect lens-free holographic images, and remote image transmission of the collecting device is realized.
5. The device for acquiring a lensless holographic image of fungal spores in air according to claim 4, wherein the control device (4) is connected with the CMOS camera (11) through a USB data line and a raspberry pie.
6. The device for collecting lens-free holographic images of fungal spores in air as claimed in claim 1, wherein said L ED light source (9) is a monochromatic coherent light source and is the only light source of the whole device, and is packaged in a closed shell with only a small hole (10) at the lower part, and the light beam passing through the small hole (10) completely covers the detection area of the CMOS camera (11).
7. The device for collecting lens-free holographic images of fungal spores in air as claimed in claim 1, wherein the collection air duct of the spore collection air duct device (3) is in a right-angle form of upper vertical section-horizontal section-lower vertical section in the air box (19) from the air inlet (20) to the blanking port (22), and the air outlet (21) of the collection air duct is opened on the corresponding side wall of the lower vertical section.
8. The device for collecting the lens-free holographic image of the fungal spore in the air as claimed in claim 1, wherein the distance between the blanking port (22) and the upper surface of the spore conveying belt (13) is 1-3 mm.
9. The device for lens-free holographic image collection of fungal spores in air as claimed in claim 1, wherein the upper surface of the spore conveyor belt (13) is provided with an adhesive coating for adhering spores.
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CN212159587U (en) * | 2020-05-29 | 2020-12-15 | 西北农林科技大学 | Non-lens holographic image collecting device for fungal spores in air |
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2020
- 2020-05-29 CN CN202010478674.8A patent/CN111505007A/en active Pending
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US20120148141A1 (en) * | 2010-12-14 | 2012-06-14 | Aydogan Ozcan | Compact automated semen analysis platform using lens-free on-chip microscopy |
CN203259479U (en) * | 2013-05-06 | 2013-10-30 | 上海创塔电子科技有限公司 | Spore monitoring warning device |
CN207007729U (en) * | 2017-07-19 | 2018-02-13 | 浙江托普云农科技股份有限公司 | A kind of spore collection and detection device |
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