CN109491069B - Device and method for remotely acquiring microscopic images of fungal spores in air - Google Patents

Abstract

The invention provides a method for remotely acquiring microscopic images of fungal spores in air, wherein a glass slide can be automatically replaced, spore adsorbents can be automatically and uniformly coated, different capturing time can be set during aerial spore capturing, the microscopic images can be acquired in real time by amplifying the fungal spores by 100-400 times according to requirements, the remote wireless transmission of image information can be realized through a wireless transmission technology, and the continuous monitoring of the fungal spores is realized through solar power supply; the invention also provides a device for realizing the method, which comprises a slide glass taking device, an object stage, a greasing device, a spore capturing air channel device, a microscopic imaging device and a solar power supply module, and is provided with an integrated wireless communication module, so that the remote wireless transmission of image information can be realized.

Description

Device and method for remotely acquiring microscopic images of fungal spores in air

Technical Field

The invention belongs to the technical field of intelligent agriculture and bioscience, and particularly relates to a device and a method for remotely acquiring microscopic images of fungal spores in air.

Background

The device is mainly used for remotely acquiring plant pathogenic fungal spore microscopic images which are transmitted and infected along with air, and further measuring the quantity of fungal spores in a plant production environment, can provide basic data for prediction and forecast of plant fungal diseases, and is an important basis for making a correct prevention and control strategy and taking prevention and control measures as soon as possible.

With the updating and popularization of spore traps, spore traps are mostly adopted for sampling and monitoring airborne plant pathogenic fungi spores. However, in the process of capturing fungal spores in field fields, the measuring points of the spore trap are scattered, unattended, large in quantity and remote in geographical position under many conditions. The traditional spore trapping method has the defects of large workload, low efficiency, time and labor waste, manual regular replacement of slides or trapping belts and the like, and cannot automatically, real-timely and large-scale rapidly monitor the concentration condition of the aerial spores in the farmland, so that the measurement result cannot reflect the real-time and dynamic change condition of the fungal spores in the large-scale farmland. Plant protection experts are keenly looking for a solution that can automatically capture and remotely count the captured results in real time.

The invention provides a device for automatically capturing and snapshotting a spore image, which is named as an integrated intelligent spore capturing device and is filed by inventor Rodripine with Chinese application number of 201010178307.2, the application date of which is 21.5.2010, but the capturing device adopts a plastic belt as a spore capturing carrier, because the spore volume is only micron, the spore can be visually seen only by optical microscopic magnification of more than 100 times, tests show that the plastic belt is easy to deform, the surface is uneven, and the automatic microscopic imaging of different areas of the plastic belt is easy to cause the failure of focusing and the blurring of the image area due to large optical magnification and small depth of field during the microscopic imaging.

Disclosure of Invention

In order to overcome the defects of the prior art, the invention aims to provide a device and a method for remotely acquiring microscopic images of fungal spores in air, which take a non-deformable glass slide as a spore capturing carrier, can automatically complete the functions of taking the glass slide, coating grease, capturing spores in the air, acquiring microscopic images of the spores, remotely transmitting and recovering the glass slide and the like, and can simplify the complexity of the device and improve the definition of the images.

In order to achieve the purpose, the invention adopts the technical scheme that:

a microscopic image remote acquisition device for fungal spores in air comprises a box body 6 and is characterized in that a glass slide taking device 1, an object stage 2, a greasing device 3, a spore capturing air channel device 4, a microscopic imaging device 5 and a solar power supply module are arranged in the box body 6, wherein the glass slide taking device 1 utilizes a stepping motor to provide glass slides 17 for the object stage 2, the greasing device 3 utilizes the stepping motor to control a gluing head 35 and a scraper blade 37 to evenly coat spore adsorbent on an acquisition area of the glass slides 17 on the object stage 2, the spore capturing air channel device 4 comprises an air box 42 without a bottom plate, an acquisition air channel air inlet 40 of the air box 42 is connected with an air inlet 9 on the box body 6 through a pipeline, an acquisition air channel air outlet 41 of the air box 42 is connected with an air suction fan 10 through a pipeline, the bottom of the air box 42 can be tightly attached to the glass slides 17 on the object stage 2 through the control of the stepping motor 38, the microscopic imaging device 5 is arranged above the object stage 2 and is used for carrying out image acquisition on a microscopic image of spores in the glass slide 17 on the microscopic imaging device.

The lower part of the box body 6 is provided with a ground bolt screw hole 7 and a universal wheel 8, and the air inlet 9 is provided with a gauze.

The slide glass taking device 1 comprises a slide storage 18 for placing a slide glass 17, the bottom of the slide storage 18 is suspended, a slide pushing plate 19 controlled by a first stepping motor 20 acts on the suspended position to horizontally push the slide glass 17 falling from the slide storage 18 out to a slide plate 22, and the slide plate 22 is controlled by a second stepping motor 21 to rotate so that the slide glass 17 slides into a slide groove 26 of the object stage 2.

The utility model discloses a spore adsorbent, including objective table 2, 16 drive lead screw guide rails, including objective table 2, four 16 drive lead screw guide rails, and objective table 2 is installed on the objective table 2, and the drive lead screw guide rails 15 makes objective table 2 remove along the X direction through three 16 drive lead screw guide rails of step motor, install step motor four 23 on the objective table 2, install spacer pin 25 and slide limiting plate 27 on the objective table 2, spacer pin 25 is controlled by five 24 of step motor, when smearing the spore adsorbent, five 24 promotion spacer pins 25 of step motor block slide 17, and slide limiting plate 27 is controlled by six 28 of step motor, pushes away slide 17 after gathering the image and makes it fall into in the recovery box.

The greasing device 3 comprises a transverse lead screw guide rail II 32, two longitudinal lead screw guide rails III 33 and a lead screw guide rail IV 34, a gluing head 35 is arranged on the lead screw guide rail III 33, a glue box 36 is arranged behind the gluing head 35, the lead screw guide rail III 33 is arranged on the lead screw guide rail II 32, a scraper blade 37 is arranged on the lead screw guide rail IV 34, a stepping motor seven 29 controls the gluing head 35 to move up and down along the lead screw guide rail III 33, a stepping motor eight 31 controls the scraper blade 37 to move up and down along the lead screw guide rail IV 34, and a stepping motor nine 30 controls the lead screw guide rail III 33 to move horizontally along the lead screw guide rail II 32.

The spore capturing air duct device 4 adopts a right-angle air duct design, so that the glass slide 17 is fully contacted with the air flow containing spores in the air box 35 to enable the air flow to be adhered with more spores, when the bottom of the air box 35 is tightly attached to the glass slide 17, the external air flow can be isolated from the interior of the box body, the external air is prevented from polluting the internal device, and the air flow in the air duct is controlled by the air suction fan 10.

The air box 42 is installed on the lead screw guide rail five 39 and driven by the stepping motor ten 38 to move up and down along the lead screw guide rail five 39, the collection air channel air inlet 40 is located at the top of the air box 42, and the collection air channel air outlet 41 is located on the side face of the air box 42.

The microscopic imaging device 5 comprises a microscope digital camera 43, a lens barrel 44 and an objective lens 45, wherein the microscope digital camera 43 is connected with an embedded industrial personal computer through a USB data line, receives an image acquisition signal sent by the industrial personal computer at any time, and can magnify by 100-400 times through the lens barrel 44 and the objective lens 45 to capture microscopic images of fungal spores on the glass slide 17.

The microscope light source 12 is installed to objective table 2 below just right microscopic imaging device 5, 6 tops of box set up solar energy electroplax 14, and 14 inclination adjustable of solar energy electroplax, and inside sets up battery 13, and battery 13 and solar energy electroplax 14 link to each other and use the clear solar energy of collection to supply power for whole device.

The microscope light source 12 is connected with the control module, and the control module adjusts output current through PWM signals with different duty ratios, so that stable and quantitative adjustment of the luminous intensity of the microscope light source 12 is achieved.

The invention adopts an embedded industrial personal computer to control each motor, the industrial personal computer is encapsulated in the box body 6 and is connected with the wireless communication module through a USB interface, the remote image transmission of the acquisition device is realized, meanwhile, the control command signal from the server end is received, and each motion module is started or suddenly stopped according to the signal of the server end.

The invention also provides an acquisition method using the device for remotely acquiring the microscopic image of the fungal spore in the air, which is controlled by an embedded industrial control machine and is characterized by comprising the following steps:

step S1: the industrial personal computer is electrified, the software is automatically started, and a link is established with the server end;

step S2: the system self-check, which sequentially sends handshake instructions to the driving module, receives feedback instructions, sends instructions to the I/O module, receives the feedback instructions, reads the setting parameters of the microscope digital camera 43, and detects whether the camera works normally;

step S3: and (4) after self-checking is completed, sending the equipment state to a server side: drive module status, I/O module status, microscope digital camera 43 parameters;

step S4: resetting each motion module through a limit switch, and waiting for an acquisition instruction of a server end;

step S5: receiving a collection instruction, operating the slide glass taking device 1, and taking out the slide glass 17;

step S6: the object stage 2 moves leftwards, and the slide glass 17 is conveyed to the greasing device 3 to be evenly smeared with the spore adsorbent;

step S7: the object stage 2 moves leftwards, the glass slide 17 is horizontally conveyed to the position of the spore trapping air channel device 4, the spore trapping air channel device 4 operates to enable the bottom of the air box 42 to be tightly attached to the glass slide 17, and meanwhile, the air suction fan 10 is started;

step S8: the time range for capturing the fungal spores is 2-24 hours, and the capturing time can be set according to actual requirements;

step S9: after the capture is finished, the air suction fan 10 is closed, the objective table 2 moves leftwards, the slide glass 17 is horizontally conveyed to the microscopic imaging position 5, and the microscope light source 12 is started;

step S10: controlling the object stage 2 to move in a plane so as to collect spore microscopic images at different positions on the glass slide 17, and simultaneously starting the microscope digital camera 43 to collect the microscopic images;

step S11: the industrial personal computer stores the image data in the hard disk and remotely transmits the image to the server end through a wireless network;

step S12: turning off the microscope light source 12, controlling the stepping motor six 28 to move to convey the slide 17 to a recovery position for discarding, and finishing the collection;

and looping steps S2-S12.

Compared with the prior art, the invention has the beneficial effects that:

according to the device and the method for remotely acquiring the microscopic image of the fungal spore in the air, disclosed by the invention, the glass slide can be automatically replaced, different capturing time can be set during the capture of the fungal spore in the air, the microscopic image can be acquired in real time by amplifying the fungal spore by 100-400 times, and the device is provided with the integrated wireless communication module, so that the remote wireless transmission of image information can be realized. The power is supplied by solar energy, so that the continuous monitoring of the fungal spores is realized. The method can timely and accurately capture and monitor fungal spores in the air, can provide basic data for prediction and forecast of plant fungal diseases, and is an important basis for making a correct prevention and control strategy and taking prevention and control measures as soon as possible.

Drawings

FIG. 1 is a schematic structural diagram of each module of the apparatus of the present invention.

FIG. 2 is a schematic view of the main structure of the apparatus of the present invention.

FIG. 3 is a schematic view of a slide taking device according to the present invention.

Fig. 4 is a schematic view of the stage of the present invention.

FIG. 5 is a schematic view of a fat liquoring device of the present invention.

Fig. 6 is a schematic view of the collecting duct device of the present invention.

FIG. 7 is a schematic view of a microscopic imaging apparatus of the present invention.

Fig. 8 is a software flow chart of the remote acquisition device of the present invention.

Detailed Description

The following describes the embodiment of the invention in detail by taking the embodiment of collecting wheat stripe rust germ summer spore and selecting Vaseline as an adsorbent.

As shown in figure 1, the invention is a schematic diagram of the structure of each module of the microscopic image remote acquisition device of fungal spores in air, the device takes an embedded industrial personal computer as a control core, the wireless communication module receives a control instruction of the server end, the wireless communication module and the camera are connected with the embedded industrial personal computer through a USB line, the driving module and the I/O module are connected with the embedded industrial personal computer through a communication 485 bus to realize signal transmission, the driving module adjusts the driving chip to output different pulse numbers through PWM signals, thereby realizing the motion of the motor module, the limit switch is connected with the driving module to control the start and stop of the motor, the I/O module is connected with the air suction fan and the control module, the control module adopts a PT4115 chip with adjustable output current, the output current is adjusted by outputting PWM signals with different duty ratios, so that the stable and quantitative adjustment of the luminous intensity of the microscope light source is realized. The solar power supply module supplies power to all modules of the device.

FIG. 2 is a schematic view of the main structure of the apparatus of the present invention; FIG. 3 is a schematic view of a slide taking device of the present invention; FIG. 4 is a schematic view of an object table according to the present invention; FIG. 5 is a schematic view of a fat liquoring device according to the present invention; FIG. 6 is a schematic view of an air collection duct assembly according to the present invention; FIG. 7 is a schematic view of a microscopic imaging apparatus of the present invention.

As shown in fig. 2, fig. 3, fig. 4, fig. 5, fig. 6 and fig. 7, the device for remotely acquiring microscopic images of fungal spores in air provided by the invention comprises a slide glass taking device 1, an object stage 2, a greasing device 3, a spore capturing air duct device 4, a microscopic imaging device 5 and a solar power supply module; the remote acquisition device is arranged in the box body 6, and the bottom of the box body 6 is provided with a ground bolt screw hole 7 and a universal wheel 8; the slide glass taking device 1 comprises a slide library 18 for placing a slide glass 17, the bottom of the slide library 18 is suspended, when the device 1 runs, a first stepping motor 20 firstly drives a slide pushing plate 19 to move forwards to act on the suspended position to push the slide glass 17 in the slide library 18 to move to a slide plate 22, and at the moment, a second stepping motor 21 drives the slide plate 22 to incline for a certain angle to enable the slide glass 17 to slide to a slide groove 26 on the object stage 2; the greasing device 3 comprises a transverse lead screw guide rail II 32, two longitudinal lead screw guide rails III 33 and a lead screw guide rail IV 34, a gluing head 35 is arranged on the lead screw guide rail III 33, a glue box 36 is arranged behind the gluing head 35, the lead screw guide rail III 33 is arranged on the lead screw guide rail II 32, a scraper blade 37 is arranged on the lead screw guide rail IV 34, a stepping motor VII 29 controls the gluing head 35 to move up and down along the lead screw guide rail III 33, a stepping motor VII 30 controls the lead screw guide rail III 33 to move horizontally along the lead screw guide rail II 32, and a stepping motor VIII 31 controls the scraper blade 37 to move up and down along the lead screw guide rail IV 34, so that vaseline is uniformly smeared on a glass slide 17 acquisition area; the spore capturing air channel device 4 comprises an air box 42 without a bottom plate, an air inlet 40 of an air collecting channel of the air box 42 is connected with an air inlet 9 at the top of the box body 6 through a hose, an air outlet 41 of the air box 42 is connected with an air suction fan 10 through a hose, when spore collection is started, a stepping motor ten 38 is started to enable a lead screw guide rail five 39 to rotate to drive the air box 42 to descend, so that the bottom of the air box 42 is tightly attached to the glass slide 17 and then stops, the air suction fan 10 is started at the moment, negative pressure is formed in the air channel, air mixed with spores is sucked from the air inlet 9 and discharged from an air outlet 11, and the spores in the air collide with the glass slide 17 and are adhered to the glass slide 17 along with the movement of the air flow in the air box 42; the microscopic imaging device 5 is arranged above the objective table 2 and comprises a microscope digital camera 43, a lens barrel 44 and an objective lens 45, wherein the microscope digital camera 43 is connected with the embedded industrial personal computer through a USB data line, receives an image acquisition signal sent by the embedded industrial personal computer at any time, and captures microscopic images of summer spores in the slide glass on the objective table 2 by amplifying the image acquisition signal by 200 times through the lens barrel 44 and the objective lens 45 according to the sizes of the summer spores. The embedded industrial personal computer and the circuit module are loaded in the box body 6. The collection device adopts a 12V storage battery 13 to provide a power supply, and the storage battery 13 is connected with a solar panel 14 to collect clean solar energy to supply power to the whole device.

In order to automatically complete the operation functions of taking a glass slide, coating grease, capturing summer spores in the air, collecting spore microscopic images, recovering the glass slide and the like, the method is specifically implemented as follows: the object stage 2 is arranged on the first lead screw guide rail 15, the first lead screw guide rail 15 is driven by the third stepping motor 16 to enable the object stage 2 to move along the X direction, and the functions of taking a glass slide, coating grease, collecting summer spores in the air and the like are automatically completed; meanwhile, a stepping motor IV 23 is further mounted on the object stage 2, so that the object stage 2 can move along the Y direction. The stepping motors 16 and 23 receive pulse signals output by the industrial personal computer to drive the object stage 2 to realize plane motion in the direction of X, Y so as to acquire spore microscopic images at different positions on the slide glass. The glass slide recovery box is characterized in that a limiting pin 25 and a glass slide limiting plate 27 are mounted on the objective table 2, the limiting pin 25 is controlled by a five-step motor 24, when vaseline is smeared, the five-step motor 24 pushes the limiting pin 25 to clamp the glass slide 17, the glass slide is prevented from being stuck and taken away by the glue spreading head 35 when the vaseline is smeared, the glass slide limiting plate 27 is controlled by a six-step motor 28, and after the image collection is finished, the glass slide 17 is pushed to fall into the recovery box.

According to the device for remotely acquiring the microscopic images of the fungal spores in the air, the embedded industrial personal computer is connected with the wireless communication module through the USB interface, so that the remote image transmission of the acquisition device is realized. The wireless communication module not only receives image transmission signals sent by an industrial personal computer, but also receives control command signals from the server, and starts or suddenly stops each motion module according to the signals of the server, for example, the server finds that no spore picture is received for a long time, and the movement of the object stage can be suspended so as to save electric quantity.

According to the device for remotely collecting the microscopic images of the fungal spores in the air, which is provided by the invention, the spore capturing air channel device 4 adopts a right-angle air channel design, so that the glass slide 17 is fully contacted with the airflow containing the spores in the air box 35 to enable the air slide to be adhered with more spores. When the bottom of the bellows 35 is in close contact with the slide 17, the external air flow is isolated from the interior of the case, preventing the external air from contaminating the internal devices. Meanwhile, the air flow in the air duct can be controlled by the suction fan 10.

According to the device for remotely acquiring the microscopic images of the fungal spores in the air, the slide library 18 is vertically stacked for storage, 100 slides can be stored to the maximum extent, and the device is used for remotely acquiring spore image data continuously in the field for a long time.

According to the device for remotely acquiring the microscopic image of the fungal spore in the air, which is provided by the invention, the acquisition device needs to work in the field with a severe environment for a long time, and in consideration of the use requirements of dust prevention, water prevention and moisture prevention, the box body 6 is designed into a sealed box body with a door by adopting 304 stainless steel materials, so that the box body is prevented from being rusted and corroded to cause water leakage and damage internal equipment, and the air inlet 9 at the top of the box body is provided with a gauze. Wind-resistant design is also considered for the device used in the field for a long time, so that a bolt hole is reserved at the bottom of the box body, and the device is convenient for fixing ground legs when needed.

As shown in fig. 8, the steps of the software workflow of the device for remotely acquiring microscopic images of fungal spores in air provided by the invention are as follows:

step S1: the industrial personal computer is electrified, the software is automatically started, and a link is established with the server end;

step S2: the system self-check, which sequentially sends handshake commands to the driving module, receives feedback commands, sends commands to the I/O module, receives feedback commands, reads the setting parameters (exposure time and gain) of the microscope digital camera 43, and detects whether the camera works normally;

step S3: and (4) after self-checking is completed, sending the equipment state to a server side: drive module status, I/O module status, microscope digital camera 43 parameters (exposure time, gain);

step S4: resetting each motion module through a limit switch, and waiting for an acquisition instruction of a server end;

step S5: receiving a collection instruction, operating the slide glass taking device 1, and taking out the slide glass 17;

step S6: the object stage 2 moves leftwards, and the glass slide 17 is conveyed to the greasing device 3 to be evenly smeared with vaseline;

step S7: the object stage 2 moves leftwards, the glass slide 17 is horizontally conveyed to the spore collecting air channel position 4, the spore capturing air channel device 4 operates to enable the bottom of the air box 42 to be tightly attached to the glass slide 17, and meanwhile, an air suction fan is started;

step S8: the summer spore is captured for 2 hours;

step S9: after the capture is finished, the air suction fan 10 is closed, the objective table 2 moves leftwards, the slide glass 17 is horizontally conveyed to the microscopic imaging position 5, and the microscope light source 12 is started;

step S10: controlling the object stage 2 to move in a plane so as to collect the summer spore microscopic images at different positions on the glass slide 17, and simultaneously starting the microscope digital camera 43 to collect the microscopic images;

step S11: the industrial personal computer stores the image data in the hard disk and remotely transmits the image to the server end through a wireless network;

step S12: the microscope light source 12 is turned off, the stepping motor six 28 is controlled to move to convey the slide 17 to the recovery position to be discarded, and the collection is finished. And circulating the steps S2-S12, and automatically replacing the functions of glass slide taking, vaseline coating, spore capturing, microscopic imaging, image remote transmission and the like. The functions of automatically finishing the slide taking, the greasing, the spore collection in the air, the spore microscopic image collection, the remote transmission, the slide recovery and the like are realized.

The above description of the present invention is intended to be illustrative. Various modifications, additions and substitutions for the specific embodiments described may be made by those skilled in the art without departing from the scope of the invention as defined in the accompanying claims.

Claims (7)

1. A microscopic image remote acquisition device for fungal spores in air comprises a box body (6), and is characterized in that a glass slide sheet taking device (1), an object stage (2), a greasing device (3), a spore capturing air channel device (4), a microscopic imaging device (5) and a solar power supply module are arranged in the box body (6); wherein the slide glass taking device (1) provides a slide glass (17) to the object stage (2) by utilizing a stepping motor; the greasing device (3) controls the gluing head (35) and the scraper blade (37) by using a stepping motor to evenly paint the spore adsorbent on the collection area of the glass slide (17) on the objective table (2); the spore capturing air channel device (4) comprises an air box (42) without a bottom plate, an air inlet (40) of a collecting air channel of the air box (42) is connected with an air inlet (9) on the box body (6) through a pipeline, an air outlet (41) of the collecting air channel of the air box (42) is connected with an induced draft fan (10) through a pipeline, and the bottom of the air box (42) can be tightly attached to a glass slide (17) on the objective table (2) through ten (38) control of a stepping motor; the microscopic imaging device (5) is arranged above the objective table (2) and is used for collecting microscopic images of spores in a glass slide (17) on the microscopic imaging device;

wherein:

the slide glass taking device (1) comprises a slide library (18) for placing a slide glass (17), the bottom of the slide library (18) is suspended, a slide pushing plate (19) controlled by a first stepping motor (20) acts on the suspended position to horizontally push out the slide glass (17) falling from the slide library (18) to a slide sliding plate (22), and the slide sliding plate (22) is controlled by a second stepping motor (21) to rotate so that the slide glass (17) slides into a slide groove (26) of the objective table (2);

the object stage (2) is mounted on a first screw guide rail (15), the first screw guide rail (15) is driven by a third stepping motor (16) to enable the object stage (2) to move along the X direction, a fourth stepping motor (23) is mounted on the object stage (2), the fourth stepping motor (23) drives the object stage (2) to move along the Y direction, a limiting pin (25) and a slide limiting plate (27) are mounted on the object stage (2), the limiting pin (25) is controlled by a fifth stepping motor (24), when spore adsorbent is coated, the fifth stepping motor (24) pushes the limiting pin (25) to clamp the slide (17), the slide limiting plate (27) is controlled by a sixth stepping motor (28), and after the image collection is finished, the slide (17) is pushed to fall into a recovery box;

the greasing device (3) comprises a transverse lead screw guide rail II (32), two longitudinal lead screw guide rails III (33) and a lead screw guide rail IV (34), a gluing head (35) is arranged on the lead screw guide rail III (33), a gluing box (36) is arranged behind the gluing head (35), the lead screw guide rail III (33) is arranged on the lead screw guide rail II (32), a scraper blade (37) is arranged on the lead screw guide rail IV (34), a stepping motor seven (29) controls the gluing head (35) to move up and down along the lead screw guide rail III (33), a stepping motor eight (31) controls the scraper blade (37) to move up and down along the lead screw guide rail IV (34), and a stepping motor nine (30) controls the lead screw guide rail III (33) to move horizontally along the lead screw guide rail II (32);

the spore capturing air channel device (4) adopts a right-angle air channel design, so that the glass slide (17) is in full contact with the airflow containing spores in the air box (42) to enable the air slide to be adhered with more spores, when the bottom of the air box (42) is tightly adhered to the glass slide (17), the external airflow can be isolated from the interior of the box body, the external air is prevented from polluting the internal device, and the size of the airflow in the air channel is controlled by the air suction fan (10);

bellows (42) are installed on lead screw guide rail five (39), are driven by step motor ten (38) and move up and down along lead screw guide rail five (39), gather wind channel air intake (40) and be located the top of bellows (42), gather wind channel air outlet (41) and be located the side of bellows (42).

2. The device for remotely acquiring microscopic images of fungal spores in air according to claim 1, wherein a geodesic screw hole (7) and a universal wheel (8) are arranged at the lower part of the box body (6), and a gauze is arranged at an air inlet (9) on the box body (6).

3. The device for remotely acquiring microscopic images of fungal spores in air according to claim 1, wherein the microscopic imaging device (5) comprises a microscope digital camera (43), a lens barrel (44) and an objective lens (45), the microscope digital camera (43) is connected with an embedded industrial personal computer through a USB data line, receives image acquisition signals sent by the industrial personal computer at any time, and can be amplified by 100-400 times through the lens barrel (44) and the objective lens (45) to capture microscopic images of the fungal spores on the glass slide (17).

4. The device for remotely acquiring microscopic images of fungal spores in air according to claim 3, wherein a microscope light source (12) is arranged under the object stage (2) and is opposite to the microscopic imaging device (5), a solar panel (14) is arranged at the top of the box body (6), the inclination angle of the solar panel (14) can be adjusted, a storage battery (13) is arranged inside the box body, and the storage battery (13) is connected with the solar panel (14) to acquire clean solar energy to supply power to the whole device.

5. The device for remotely acquiring microscopic images of fungal spores in air according to claim 4, wherein the microscope light source (12) is connected with the control module, and the control module adjusts the output current through PWM signals with different duty ratios to realize stable and quantitative adjustment of the luminous intensity of the microscope light source (12).

6. The device for remotely acquiring microscopic images of fungal spores in air according to claim 1, is characterized in that an embedded industrial personal computer is adopted to control each motor, is packaged in the box body (6) and is connected with the wireless communication module through a USB interface to realize the remote image transmission of the acquisition device, receives a control command signal from a server end at the same time, and starts or suddenly stops each motion module according to the signal of the server end.

7. The method for collecting the microscopic image of the fungal spores in the air by using the device for remotely collecting the microscopic image of the fungal spores in the air as claimed in claim 4 is controlled by an embedded industrial control machine, and is characterized by comprising the following steps of:

step S1: the industrial personal computer is electrified, the software is automatically started, and a link is established with the server end;

step S2: the system self-check is used for sequentially sending handshake instructions to the driving module, receiving feedback instructions, sending instructions to the I/O module, receiving the feedback instructions, reading the setting parameters of the microscope digital camera (43), and detecting whether the camera works normally;

step S3: and (4) after self-checking is completed, sending the equipment state to a server side: drive module status, I/O module status, microscope digital camera (43) parameters;

step S4: resetting each motion module through a limit switch, and waiting for an acquisition instruction of a server end;

step S5: receiving a collection instruction, operating the slide glass taking device (1) and taking out the slide glass (17);

step S6: the objective table (2) moves leftwards, and the slide glass (17) is conveyed to the greasing device (3) to be evenly smeared with the spore adsorbent;

step S7: the objective table (2) moves leftwards, the glass slide (17) is horizontally conveyed to the position of the spore capturing air channel device (4), the spore capturing air channel device (4) operates to enable the bottom of the air box (42) to be tightly attached to the glass slide (17), and meanwhile, the air suction fan (10) is started;

step S8: the time range of capturing the fungal spores is 2-24 hours, and the capturing time is set according to actual requirements;

step S9: after the capture is finished, the air suction fan (10) is closed, the objective table (2) moves leftwards, the glass slide (17) is horizontally conveyed to the microscopic imaging device (5), and the microscope light source (12) is started;

step S10: controlling the object stage (2) to move in a plane so as to collect spore microscopic images at different positions on the glass slide (17), and simultaneously starting a microscope digital camera (43) to collect microscopic images;

step S11: the industrial personal computer stores the image data in the hard disk and remotely transmits the image to the server end through a wireless network;

step S12: turning off a microscope light source (12), controlling a stepping motor six (28) to move to convey the slide glass (17) to a recovery position for discarding, and finishing collection;

the steps S2 to S12 are repeated.

Images