CN111500505A - Intestinal tract repairing method for reducing heavy metal residues in vivo by using probiotics - Google Patents
Intestinal tract repairing method for reducing heavy metal residues in vivo by using probiotics Download PDFInfo
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- CN111500505A CN111500505A CN202010432513.5A CN202010432513A CN111500505A CN 111500505 A CN111500505 A CN 111500505A CN 202010432513 A CN202010432513 A CN 202010432513A CN 111500505 A CN111500505 A CN 111500505A
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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Abstract
The invention provides an intestinal tract repairing method for reducing heavy metal residue in vivo by using probiotics, which is characterized by comprising the following steps: (1) separating microorganisms from yogurt in the grazing land of Qinghai-Tibet plateau; (2) preparing an MRS culture medium and a PBS solution; (3) inoculating and culturing; (4) detecting strains; (5) analyzing the antioxidant capacity of the probiotics; (6) analyzing the heavy metal resistance of the probiotics; (7) and (4) analyzing the heavy metal adsorption capacity of the probiotics. The method applies the probiotics with strong oxidation resistance to the preparation of the yoghourt, and reduces the oxidation pressure in the intestinal tract by eating the probiotic yoghourt, thereby protecting the intestinal flora and leading the microbial community to better play a role in reducing the heavy metal residue.
Description
Technical Field
The invention relates to the field of microbial genetic engineering, in particular to a probiotic strain and an intestinal tract repair method for reducing heavy metal residues in vivo by using the probiotic strain.
Background
Heavy metal contamination has become a global problem. In recent years, under the influence of discharge of heavy metal industrial wastewater and daily activities of human beings, about 46700hm is generated every year in China2The land is contaminated with heavy metals. Heavy metals enter human bodies through food chains, and pose serious threats to human health, for example, cadmium poisoning can cause diseases such as osteoporosis, arthralgia and renal failure, and chromium poisoning can cause damage to liver, kidney and endocrine glands, so that the diseases are diseased. At present, heavy metal pollution in soil is mainly carried out by methods such as physical remediation, chemical remediation, phytoremediation and the like, but the methods have the problems of time consumption, high cost, secondary pollution and the like. In addition, the existing heavy metal restoration is mainly carried out in the environment, and the restoration area of the methods is extremely limited in consideration of the large-area heavy metal pollution range in the world, so that the human beings are still inevitably threatened by the heavy metals. Therefore, a safe and effective heavy metal remediation method is urgently needed to be found.
The intestinal tract is the largest immune organ of the human body, and has important functions of improving the immunity of the human body besides functions of digestion, absorption and secretion. At least 1000 different types of microorganisms exist in the intestinal tract of a human body, and the microorganisms play an important role in regulating the functions of the intestinal tract, and researches show that sterile mice have nerve development defects, poor lymph development and low immune function compared with normal mice. Intestinal microorganisms affect various physiological activities such as development, absorption, immunity and the like of the intestinal tract. Thus, the function of the gut can be improved by modulating the gut microflora composition.
The probiotics are active microorganisms which colonize in human intestinal tracts and are beneficial to organisms, and the Tibetan plateau probiotics can not only directly repair heavy metal toxicity, but also indirectly repair the heavy metal toxicity by adjusting (/ remodeling) intestinal microflora. The method for repairing the intestinal tract by using the probiotics is safe and effective, simple and convenient to operate, low in price and long in lasting action time, not only can reduce the residue of heavy metal in a body, but also can enhance the immunity of a human body by regulating the composition and metabolism of intestinal flora.
The problems existing in the prior art are as follows:
(1) at present, in clinical practice, the method for detoxifying human body weight metal mainly uses a chelating agent for treatment, but the method has large side effects, such as the risks of carcinogenesis and teratogenesis, and simultaneously, due to the difficult degradability of most chelating agents, secondary pollution is caused to the environment after the chelating agents are discharged from the body through intestinal tracts.
(2) At present, extracts with oxidation resistance are also utilized to play a role in oxidation resistance, for example, extracts of fungi such as ganoderma lucidum spore powder and truffle have strong oxidation resistance; the plant extracts of ramulus Mori, folium Pruni and folium Ziziphi Spinosae also have antioxidant effect, and can be administered to reduce oxidation pressure in intestinal tract. However, compared with probiotics, the extraction process of the extract is more complicated, the preparation cost is higher, the acting time in the gastrointestinal tract is shorter, and the extract needs to be eaten for a long time.
(3) In the existing method for reducing the residual of heavy metals in vivo by using probiotics, the selected strain basis is the adsorption effect on the heavy metals, and a single strain is aimed at a single heavy metal. But the environment is polluted by a plurality of heavy metals at present, so the action range of removing the heavy metals by the existing probiotics is smaller.
Disclosure of Invention
In view of the defects of the prior art, the invention provides an intestinal tract repairing method for reducing heavy metal residues in vivo by using probiotics. A new strain is screened out, and the intestinal tract repairing method for reducing heavy metal residues in vivo by using the strain is provided. The invention adopts the following technical scheme:
1. a probiotic strain capable of reducing heavy metal residues in vivo, wherein the probiotic strain is Pediococcus acidilactici (Pediococcus acidilactici) QZ-01, which is deposited in China Center for Type Culture Collection (CCTCC) at 11 months and 14 days in 2019, and the address is as follows: in Wuhan university school of Wuhan 299 in Wuchang district of Wuhan city, Hubei province, the preservation number is CCTCCNO: m2019930.
2. A separation method of probiotics capable of reducing heavy metal residues in vivo comprises the following steps:
(1) separating microorganisms from yogurt in the grazing land of Qinghai-Tibet plateau; (2) preparing an MRS culture medium and a PBS solution; (3) inoculating and culturing; (4) detecting strains; (5) analyzing the antioxidant capacity of the probiotics; (6) analyzing the heavy metal resistance of the probiotics; (7) and (4) analyzing the heavy metal adsorption capacity of the probiotics.
3. And (3) comprehensively selecting a strain pediococcus acidilactici QZ-01 with high heavy metal resistance and oxidation resistance from the screened probiotics for preparing the functional yogurt in the next step.
4. A method for preparing yoghourt for reducing heavy metal residues in vivo comprises the following steps:
(1) activation of probiotic strains; (2) fermenting and making yoghourt; (3) and (4) carrying out experimental analysis on the yoghourt animal.
5. The application of the probiotics QZ-01 in reducing heavy metals in vivo comprises but is not limited to Cr, Hg, Pb and Ni.
6. Use of probiotic QZ-01 for scavenging hydroxyl and DPPH radicals.
7. Application of probiotic QZ-01 in adsorption of Cr, Hg, Pb and Ni.
8. Application of probiotic QZ-01 in repairing of Cr-induced liver oxidative stress and injury.
9. The application of the probiotics QZ-01 in reducing the abundance of harmful bacteria and increasing the abundance of beneficial bacteria.
10. The probiotic QZ-01 can be used for up-regulating and expressing the genes related to Cr reduction and oxidation resistance in the intestinal microflora.
11. The probiotic QZ-01 up-regulates the Cr resistance and the reduction capability of the expressed gene with unknown function.
12. Application of probiotic QZ-01 in reducing intestinal tract repair of heavy metal residues in vivo.
Has the advantages that:
the application provides a new bacterial strain, and is applied to the preparation of acidophilus milk through this bacterial strain, through edible probiotic acidophilus milk reduction oxidation pressure in the intestinal to protection intestinal flora makes better performance of microbial community reduce the effect that the heavy metal is remained, and this kind of method has the broad spectrum nature to the getting rid of heavy metal, and it is effectual to get rid of, easy operation, and the low price, and can establish advantages such as continuously playing the effect in the intestinal.
Drawings
FIG. 1 shows the results of the isolation of probiotics according to the invention;
FIG. 2 shows the preparation result of the yogurt of the present invention;
FIG. 3 shows the hydroxyl radical and DPPH radical scavenging ability of different probiotics according to the invention;
among them, a hydroxyl radical (A) and a DPPH radical (B).
FIG. 4 shows the results of the resistance of different probiotics of the present invention to heavy metals Cr, Hg, Pb, Ni;
wherein, Cr (A), Hg (B), Pb (C), Ni (D).
FIG. 5 shows experimental groupings of animals according to the present invention;
FIG. 6A shows the daily food intake of a mouse according to the present invention; b is the influence of Cr (VI) and probiotics on the growth rate of the mouse;
FIG. 7 is a graph of the effect of probiotic QZ-01 and XS40 treatment of the invention on the chromium content in mouse feces and tissues;
among these, mouse feces (a) and tissues (B).
FIG. 8 shows the repairing effect of the probiotics QZ-01 and XS40 of the invention on the liver injury of mice caused by Cr (VI);
FIG. 9A is a graph showing the results of a quantitative study of QZ-01 in feces from probiotic QZ-01 treated groups of the present invention; b is the content of QZ-01 in the excrement after 4 different treatments for 20 days; c and D are the reducing capacity of fecal culturable microorganisms to Cr (VI) at 37 ℃ for 72 hours and 44 hours, respectively;
FIG. 10 is the composition of total bacteria and metabolically active bacteria in feces of the invention;
wherein (A) the ratio of firmicutes to bacteroidetes; (B) the relative abundance of the bacterial 16SrRNA gene at the family level; (C) genus level heatmap based on 16S rRNA sequencing; (D) the classification assignment of mRNA at the genus level for each sample.
FIG. 11 is an analysis of the resistance and reducing power of the probiotic QZ-01 of the present invention to Cr (VI) of an unknown functional gene with increased differential expression of gut microbes;
the method comprises the following steps of (A) distribution of Cr repair related genes in the DEGS, (B) growth of engineering escherichia coli strains in L B culture medium containing 1Mm Cr (VI), (C) Cr (VI) reduction rate of engineering escherichia coli B L21 containing GCrR genes, (D) transmission electron microscopy and energy spectrum analysis of engineering escherichia coli treated and untreated with 1mM Cr (VI), (E) evolutionary relationship of GCrR and known chromate reductase of different genera, (F) GCrR homologous sequence analysis, and (G) structural modeling of 53431.
FIG. 12 is a schematic illustration of the mechanism of "bowel repair" according to the present invention;
FIG. 13 is a report of liver and kidney function test of the same school A;
FIG. 14 is a report of routine blood tests of the same school of A.A. in accordance with the present invention;
FIG. 15 is a report of the liver function and kidney function test of the same student class B; (ii) a
FIG. 16 is a report of routine blood tests of the same school according to the present invention;
FIG. 17 is the liver and kidney function test report of the third school student of the invention
FIG. 18 is a report of a routine blood test of the third school of the invention;
FIG. 19 is a report of liver and kidney function tests of Ding classmate in accordance with the present invention;
FIG. 20 is a report of a routine test of blood of the same school according to the present invention;
FIG. 21 is a report of liver and kidney function tests of the same student of the invention;
FIG. 22 is a report of routine blood tests of the pentastudy of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, embodiments of the present invention are described in detail below with reference to the accompanying drawings. Examples of these preferred embodiments are illustrated in the accompanying drawings. The embodiments of the invention shown in the drawings and described in accordance with the drawings are exemplary only, and the invention is not limited to these embodiments.
It should be noted that, in order to avoid obscuring the technical solution of the present invention with unnecessary details, only the structures and/or processing steps closely related to the solution according to the present invention are shown in the drawings, and other details that are not relevant are omitted.
Example 1
The embodiment provides a separation method capable of reducing in-vivo heavy metal residual probiotics, which comprises the following steps:
1. isolation of microorganisms from yogurt from the grazing land of Qinghai-Tibet plateau
The yogurt is prepared by herdsmen on Qinghai-Tibet plateau. Firstly, collecting fresh yak milk, pouring the yak milk into a pot, cooking with small fire, wherein the temperature cannot be too high and the yak milk cannot scald the hands, and cooking for later use. Taking a proper amount of the prepared yoghourt in the previous day, adding the prepared yoghourt into the boiled fresh milk, uniformly stirring, and then fermenting for 3-4 hours at the temperature of about 42 ℃.
2. MRS culture medium and PBS solution are prepared
Putting 63.3g MRS agar into conical flask, adding 1000m L distilled water, sealing with cotton plug, sterilizing at 121 deg.C for 20min, respectively weighing 0.8g NaCl, 0.02g KCl, and 0.364g Na2HPO4·12H2O、0.024g KH2PO4Placing into a conical flask, adding 100m L distilled water, stirring, adjusting pH to 7.4, and sterilizing.
3. Inoculation and culture
Pouring the sterilized MRS solid culture medium into culture dishes in a clean bench, pouring about 30m L into each culture dish, sealing a sealing film, inverting for later use, sucking 5m L PBS solution by using a pipette, adding the PBS solution into a test tube, mixing a small amount of yoghourt into the PBS solution for dilution, coating the diluted yoghourt on the MRS solid culture medium, and culturing at the constant temperature of 37 ℃ for 18 hours.
4. Strain detection
As shown in the attached FIG. 1 and Table 1, single colonies were obtained, and then single colonies were picked for colony PCR using 27F/1492R universal primers, dNTP mix, Taq enzyme, Taq Buffer and other reagents required for PCR were purchased from Takara Bio Inc., and the amplification procedures were 94 ℃ for 5min, (95 ℃ for 30S, 58 ℃ for 30S, 72 ℃ for 90S) for 35 cycles, and 72 ℃ for 10 min. the amplified samples were subjected to 16S rRNA sequencing, and the strains selected are shown in Table 2, including Pediococcus acidilactici (Pediococcus acidilactici), Lactobacillus fermentum (L actans fermentum), Lactobacillus plantarum (L Acobacterium), Lactobacillus acidophilus (L Acidophilus), Lactobacillus casei (L Acobacterium ei), Lactobacillus bulgaricus (L Lactobacillus bulgaricus) and the like.
5. Antioxidant capacity analysis of probiotics
The hydroxyl radical scavenging capacity of several probiotics is determined (1M L0.75 mM phenanthroline, 1.5M L0.15.15M sodium phosphate buffer and 1M L0.75 mM FeSO are respectively taken4And 1m L0.01% H2O2Preparing mixed solution, adding sample to be tested 1m L, mixing, incubating at 37 deg.C for 30min, and measuring absorbance at 536nm2O2Absorbance of (b). Hydroxy radical scavenging ability ═ aSample (I)-ABlank space]/[AControl-ABlank space]) DPPH radical scavenging ability (1 m L sample and 1m L0.2.2 mM DPPH solution (dissolved in methanol) were mixed and reacted in the dark for 30 minutes, using phosphate buffer as a blank, and the absorbance value of the mixture at 517nm was measuredBlank space-ASample (I)]/ABlank space) As shown in fig. 3: the hydroxyl radical scavenging ability and DPPH radical scavenging ability of QZ-01 are the highest, wherein the hydroxyl radical scavenging rate can reach more than 80%, and DPPH radical scavenging rate can reach more than 50%.
6. Analysis of heavy metal resistance of probiotics
Probiotics screened by MRS activationBacteria; ni to be sterilized2+、Cd2+、Cr6+、Pb2+Adding into culture medium respectively; inoculating 1% of the activated strain into each medium; the cells were incubated overnight at a constant temperature of 37 ℃ on a shaker at 150r/min and then measured for OD600 using visible light spectrophotometry, with 3 replicates per set of experiments. As shown in FIG. 4, QZ-01 is most resistant to several heavy metal ions.
7. Analysis of heavy metal adsorption Capacity of Probiotics
Culturing probiotic bacteria with MRS culture medium at 37 deg.C for 18h, centrifuging respectively, measuring dry weight and wet weight of bacteria, suspending probiotic bacteria cells with PBS buffer solution to make bacteria concentration 5 g/L (bacteria wet weight), and adding Ni into the bacteria suspension respectively2+、Cd2+、Cr6 +、 Pb2+Adjusting pH to 6.28 deg.C with NaOH and HC L to reach a final concentration of 20 mM., culturing for 2h, centrifuging, discarding supernatant, collecting precipitate, digesting with mixed solution of concentrated nitric acid and perchloric acid (volume ratio of 4: 1) at 300 deg.C for 10min, and determining the content of each heavy metal by flame atomic absorption.
The results show that: and comprehensively selecting a strain pediococcus acidilactici QZ-01 with high heavy metal resistance, high oxidation resistance and high oxidation resistance from the screened probiotics for the next step of making the functional yogurt.
Example 2
The embodiment provides a method for preparing yoghourt by reducing heavy metal residues in vivo, which comprises the following steps:
1. activation of probiotic bacterial strains
Inoculating the probiotic strain preserved on the MRS slant to skim milk (purchased from American BD company) (milk powder: water 1:8, w/v) to culture at 37-42 ℃, and performing first activation, wherein the culture time is determined according to the specific curd condition, and the culture is completed when the yoghourt is in a uniform solidification state and a small amount of whey is separated out on the surface of the curd.
2. Yoghourt fermentation preparation
Adding 3-10% of sucrose into the prepared skim milk, preheating for 10min at 50 ℃, then sterilizing at 95 ℃ for 5min, and cooling the skim milk to 42 ℃. Inoculating activated Pediococcus acidilactici, Lactobacillus plantarum, Streptococcus thermophilus and Lactobacillus bulgaricus to skim milk according to 2% -5%, and fermenting at 42 deg.C for 4-6 h. After-ripening for 12h at 4 ℃ by refrigeration. The prepared yogurt is shown in figure 2.
3. Experimental analysis of sour milk animals
Animal experiment groups are shown in figure 5, prepared yogurt is intragastrically administered to mice with an intragastric syringe needle, and the intragastric administration is continued for 30 days with 1g of yogurt per day (1 g of yogurt is dissolved in 500ul of sterile water).
(1) Daily food intake and growth rate analysis of mice
Mouse body weight and food intake were recorded weekly. The results are shown in figure 6, and the probiotics have no obvious influence on the daily food intake of the mice, but improve the growth rate of the mice.
(2) Ability of probiotics to remove heavy metals
Dissecting mouse, collecting organs (including liver, kidney, and small intestine), digesting the organs with concentrated nitric acid, and measuring heavy metal content in the organs by flame atomic absorption method. As shown in fig. 7, the heavy metal content in the liver of the mouse is reduced by about 46%, the heavy metal content in the kidney is reduced by about 43%, the heavy metal content in the small intestine is reduced by about 88%, and the heavy metal content in the feces is increased by about 27%, which indicates that the probiotic bacteria effectively reduce the heavy metal residue in the mouse.
(3) Repair effect of probiotics on mouse liver oxidation pressure and injury caused by Cr (VI)
Analysis of the repair ability of probiotics to liver and small intestine showed that pediococcus acidilactici reversed the change in the cr (vi) -induced oxidative stress marker in figure 8. Cr (vi) exposure can cause significant liver damage, including cytoplasmic vacuolization, nuclear pyknosis, and chromatin condensation, which can be mitigated by pediococcus acidilactici. Shows that QZ-01 has obvious inhibition effect on the inflammatory reaction induced by Cr (VI).
(4) Analysis of reducing power of probiotics on Cr (VI)
As shown in figure 9A, the QZ-01 begins to be planted on the 8 th day, and tends to be stable after the planting amount reaches the maximum on the 20 th day, and as shown in figure 9B, the QZ-01 in the feces of different treatment groups is quantitatively found, and the QZ-01 can be effectively planted in the intestinal tract. As shown in FIGS. 9C and 9D, analysis of the reducing power of Cr (VI) in the flora in the fecal samples of the different treatment groups showed that QZ-01 significantly improved the reducing power of Cr (VI) in the fecal flora.
(5) Analysis of gut microflora composition by 16S rRNA sequencing and Macro-transcriptome sequencing
As shown in fig. 10A, at the phylum level, the proportion of Firmicutes to Bacteroides was significantly different between the different sequencing methods, 16S rRNA sequencing analysis showed no significant difference in this proportion between the different treatment groups, macrotranscriptome sequencing analysis showed that this proportion was significantly higher in the QZ-01 repair group than in the other groups, which showed that active metabolic bacteria or play an important role in gut repair at the family level, 16S rRNA analysis showed S24-7 (12.47-42.03%), Prevotellaceae (10.88-36.07%), bacteroidacedaceae (4.57-19.66%), L acetobacter ceraceae (2.92-15.20%) and paramaprezotellaceae (0.46-15.37%) higher abundance (fig. 10B) heat map at the genus level showed that the colony of the qcr-01 repair group was close to the colony of the control group, the colony structure was significantly more relieved from the lactobacillus group, the lactobacillus group was significantly restored from the probiotic group, as shown by the relative change in fig. 0.01 to lactobacillus (0.01-7), the relative abundance was significantly increased from the corresponding to the corresponding intestinal tract infection of bacillus (0.8, the lactobacillus) and the corresponding recovery of the lactobacillus group (20, the corresponding to the corresponding intestinal tract) was significantly increased from the corresponding to the corresponding recovery of bacillus (20% of lactobacillus) (fig. 8, 7, 8-8, 8-8, 8-8, 8-8, and 8-8, 8-8% of lactobacillus-8 (7) and 8-8 (7) and similar to the corresponding recovery of lactobacillus group of lactobacillus-8 (7, and similar to the corresponding intestinal tract infections of all of lactobacillus group of lactobacillus).
(6) Probiotics QZ-01 up-regulate expression of genes related to Cr (VI) reduction and antioxidation in intestinal microflora
As shown in table 2, thioredoxin, desulfurated ferredoxin, flavoreductase, FAD/nad (p) -bound oxidoreductase, and the like, which are involved in cr (vi) reduction, are expressed in the probiotic QZ-01 up-regulated compared to the cr (vi) group. Major erythrosin protein family proteins related to oxidative stress, thiol peroxidase, erythroredoxin, peroxidase, superoxide dismutase, catalase, and the like.
(7) Analysis of Cr (VI) resistance and reducing power of QZ-01 Up-regulated expressed functionally unknown Gene
As shown in fig. 11A, 788 genes whose expression is up-regulated in QZ-01Cr (vi) were selected from 57248 single genes, including 20 known Cr (vi) reduction-related genes, 14 known anti-oxidation-related genes, 546 other annotated genes and 208 genes with unknown functions, fig. 11B is a graph in which 10 genes selected at random from 208 genes with higher abundance are subjected to gene synthesis and a corresponding e.coli engineered bacterium (uns 1-10) is constructed, and the results show that 5 of the engineered bacteria ( uns 2,6,7,8,9) significantly improve resistance to Cr (vi), it is further found that the engineered bacterium e.coli uns 9/GCrR has a stronger reducing ability to Cr (vi) (fig. 11C) is subjected to transmission scanning on e.coli GCrR, and the energy spectrum analysis shows that intracellular Cr (iii) precipitates can be analyzed in GCrR strain gcr 3, the results show that the results are expressed by electron microscopy, see that the map 11A structural analysis of gcr 3, 11B is performed by electron microscopy, 11B is a graph in which shows that the amino acid sequence comparison of gcr 3, 11B is carried out from gcr 14 Cr (gcr) and 11G — 7, 9) is carried out by electron microscopy, and 11B (gcr iii) comparison of the known amino acid reduction system, and 11B).
(8) Mechanism of intestinal tract repair with probiotics
As shown in fig. 12, cr (vi) entering the animal body increases the oxidation pressure of the intestine and liver, increases cr (vi) residue in the small intestine, liver and kidney, causes liver damage and causes intestinal flora disorder, while probiotics can effectively colonize the intestine and reduce the oxidation pressure of the intestine, protect the intestinal flora to better function, reduce cr (vi) and reduce heavy metal residue in the body, thereby protecting the liver.
(9) Safety verification of human body experiment
The normal ranges of aspartate aminotransferase and alanine aminotransferase are 1-49U/L, the normal range of total protein is 60-82U/L, the normal range of total bilirubin is 9.1-30.1U/L, as shown in figures 13, 15, 17, 19 and 21, the student test results are all in the normal range, therefore, the edible probiotic yoghourt does not damage the liver function of the human body, the normal range of urea is 2.9-7.1U/L, the normal range of creatinine is 44-108U/L, the student report results are all normal, the normal range of erythrocyte is 4-1010 ^ 9/L, the normal range of leukocyte is 3.5-5.010 ^ 12/L, the normal range of protein is 110 g/150 g/L, and the student test results of erythrocyte and the student test results are all in the normal range of 18, 18 g/3514, 18 g/18, 18 g, 14 g, 84 g, 14.
The foregoing is directed to embodiments of the present application and it is noted that those skilled in the art may make various changes and modifications without departing from the spirit and scope of the application.
TABLE 1 isolation information of the strains
TABLE 2 probiotic QZ-01 Up-regulated expression and reduction-related genes, antioxidation-related genes and partial function-unknown genes of Cr (VI)
Claims (10)
1. A probiotic strain capable of reducing heavy metal residues in vivo is Pediococcus acidilactici (Pediococcus acidilactici) QZ-01, and is preserved in CCTCC at 11 months and 14 days in 2019 with the preservation number of M2019930.
2. The method for separating probiotics capable of reducing heavy metal residues in vivo according to claim 1, comprising the following steps: (1) separating microorganisms from yogurt in the grazing land of Qinghai-Tibet plateau; (2) preparing an MRS culture medium and a PBS solution; (3) inoculating and culturing; (4) detecting strains; (5) analyzing the antioxidant capacity of the probiotics; (6) analyzing the heavy metal resistance of the probiotics; (7) and (4) analyzing the heavy metal adsorption capacity of the probiotics.
3. A method for preparing yoghourt for reducing heavy metal residues in vivo comprises the following steps: (1) activation of probiotic strains; (2) fermenting and making yoghourt; (3) and (4) carrying out experimental analysis on the yoghourt animal.
4. The use of probiotic QZ-01 isolated by the method of claim 1 for reducing heavy metals in vivo, comprising: cr, Hg, Pb, Ni.
5. Use of probiotic bacteria QZ-01 isolated by the method of claim 1 for scavenging hydroxyl radicals and DPPH radicals.
6. The use of the probiotic QZ-01 isolated by the method of claim 1 for adsorbing Cr, Hg, Pb and Ni.
7. Use of probiotic bacteria QZ-01 isolated according to the method of claim 1 for Cr-induced liver oxidative stress and injury repair.
8. The use of the probiotic QZ-01 isolated by the method of claim 1 to reduce the abundance of harmful bacteria while increasing the abundance of beneficial bacteria.
9. The use of the probiotic QZ-01 isolated according to the method of claim 1 to up-regulate genes associated with Cr reduction and antioxidant in the expressed intestinal microflora.
10. Use of probiotic QZ-01 isolated according to the method of claim 1 for reducing heavy metal residuals in the body in intestinal tract repair.
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