CN111494508B - Preparation method of traditional Chinese medicine gout plaster and product thereof - Google Patents
Preparation method of traditional Chinese medicine gout plaster and product thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/708—Rheum (rhubarb)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/076—Poria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/21—Amaranthaceae (Amaranth family), e.g. pigweed, rockwort or globe amaranth
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/489—Sophora, e.g. necklacepod or mamani
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/65—Paeoniaceae (Peony family), e.g. Chinese peony
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/86—Violaceae (Violet family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
- A61K47/38—Cellulose; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/70—Web, sheet or filament bases ; Films; Fibres of the matrix type containing drug
- A61K9/7023—Transdermal patches and similar drug-containing composite devices, e.g. cataplasms
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/06—Antigout agents, e.g. antihyperuricemic or uricosuric agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Abstract
The invention discloses a preparation method of a traditional Chinese medicine gout plaster and a product thereof, wherein the preparation method comprises the steps of mixing a rhubarb extract, a red peony root extract, a achyranthes root extract, a tuckahoe extract, a Chinese violet herb extract and a subprostrate sophora extract according to a certain mass ratio to prepare a traditional Chinese medicine extract; in the gout plaster composition, by weight, the traditional Chinese medicine extract accounts for 5-10%, the sodium carboxymethylcellulose accounts for 2-4%, the polyvinylpyrrolidone-K30 accounts for 15-20%, the ethylparaben accounts for 0.4-0.8%, the alcohol accounts for 15-18%, the glycerol accounts for 18-20%, the polyethylene glycol 400 accounts for 1.5-2%, the kaolin accounts for 10-15%, the citric acid accounts for 2-3%, the aluminum hydroxide accounts for 4-5%, and the balance is water. According to the invention, the active ingredients in the rhubarb extract, the red peony root extract, the achyranthes root extract, the tuckahoe extract, the Chinese violet extract and the subprostrate sophora extract act synergistically, and a specific preparation process is combined, so that the traditional Chinese medicine composition has a better curative effect on gout.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a preparation method of a traditional Chinese medicine gout plaster and a product thereof.
Background
Arthralgia syndrome is a syndrome of main symptoms of limb, joint, pain, soreness, numbness, stress and movement disorder caused by wind-cold-dampness-heat, and has the characteristic of gradual or repeated attack clinically. Its pathogenesis is caused by obstruction of qi and blood and failure of nourishing tendons, vessels and joints. According to the etiology and pathogenesis and clinical manifestations of arthralgia syndrome, the diseases mainly comprise rheumatism, rheumatoid arthritis, gout, sciatica, hyperosteogeny and the like.
Gout is a metabolic disease caused by increased uric acid synthesis due to abnormal purine metabolism. The onset of acute gout is manifested by severe pain, swelling, erythema, stiffness, fever, and sudden onset of symptoms in the affected joint. The onset generally lasts from several days to 1 week, and is usually not induced, but may be secondary to mild trauma or after consumption of purine-rich food. From the manifestation of gout, the attack part of gout is mainly red, swollen, hot and severe pain, and ancient people mostly belong to exogenous pathogenic factors according to syndrome differentiation.
The cataplasm is an external preparation which is prepared by taking a water-soluble polymer material as a main matrix, adding medicines, and carrying out processes such as mixing, coating, cutting and the like, and is generally applied clinically at present. The cataplasm is a novel external patch, and is deeply favored by patients due to the advantages of large drug-loading rate, wide application range, small allergic stimulation, comfortable and convenient use, low price and the like.
At present, internal medicines such as non-steroidal anti-inflammatory drugs and colchicine are commonly used for treating gout, the curative effect is reliable, the side effect is large, the gout is easy to relapse after the medicines are stopped, certain limitations and dependence are provided, and external medicines for clinical treatment are relatively lacking. Therefore, the search and development of external preparations for treating gout, especially traditional Chinese medicines, are important strategies and ways for treating and preventing gout.
Disclosure of Invention
This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. In this section, as well as in the abstract and the title of the invention of this application, simplifications or omissions may be made to avoid obscuring the purpose of the section, the abstract and the title, and such simplifications or omissions are not intended to limit the scope of the invention.
The present invention has been made keeping in mind the above and/or other problems occurring in the prior art.
Therefore, the invention aims to overcome the defects in the prior art and provide a preparation method of a traditional Chinese medicine gout patch.
In order to solve the technical problems, the invention provides the following technical scheme: a preparation method of a traditional Chinese medicine gout plaster comprises the following steps of taking a rhubarb extract, a red peony root extract, a achyranthes root extract, a poria cocos extract, a Chinese violet extract and a subprostrate sophora extract according to a mass ratio of 1-2: 2-3: 1-2: 2-3: 2-3: 1-2, mixing to obtain a traditional Chinese medicine extract; adding sodium carboxymethylcellulose into water with the mass of 10-12 times of that of the sodium carboxymethylcellulose, performing cold expansion for 10-12 hours, putting the sodium carboxymethylcellulose into a water bath kettle with the temperature of 50-55 ℃ for water bath swelling for 6-8 hours, and stirring the mixture until the mixture is uniform and transparent to be used as a phase a; mixing polyvinylpyrrolidone-K30, ethylparaben, 30-40% alcohol by volume fraction and the traditional Chinese medicine extract, and putting the mixture into a water bath kettle at the temperature of 40-55 ℃ for swelling for 8-10 hours to serve as a phase b; uniformly stirring glycerol, polyethylene glycol 400 and kaolin until no granular feeling exists, and taking the mixture as a phase c; mixing citric acid and aluminum hydroxide to obtain a d phase; adding the phase d into the phase a, fully and uniformly stirring, adding the phase c, then adding the phase b, stirring at a high speed, and carrying out ultrasonic treatment for 15min to obtain a gout plaster composition; coating the gout plaster composition, placing the gout plaster composition in an electric heating constant-temperature blast drying oven at 40-55 ℃ for drying for 8h, taking out the gout plaster composition, slightly cooling the gout plaster composition, and covering an anti-mucous membrane to obtain the traditional Chinese medicine gout plaster; the gout patch composition comprises, by mass, 5-10% of a traditional Chinese medicine extract, 2-4% of sodium carboxymethylcellulose, 15-20% of polyvinylpyrrolidone-K30, 0.4-0.8% of ethyl paraben, 15-18% of alcohol, 18-20% of glycerol, 1.5-2% of polyethylene glycol 400, 10-15% of kaolin, 2-3% of citric acid, 4-5% of aluminum hydroxide and the balance of water.
As a preferred scheme of the preparation method of the traditional Chinese medicine gout plaster, the preparation method comprises the following steps: the rheum officinale extract, the red paeony root extract, the achyranthes bidentata extract, the poria cocos extract, the Chinese violet extract and the subprostrate sophora extract are mixed according to the mass ratio of 2: 3: 1: 3: 3: 2.
as a preferred scheme of the preparation method of the traditional Chinese medicine gout plaster, the preparation method comprises the following steps: the preparation method of the rhubarb extract comprises the steps of taking rhubarb which is ground and sieved by a 20-mesh sieve, adding ethanol with the mass volume fraction of 70 percent of 20 times, carrying out reflux extraction for 2 times at the temperature of 60 ℃ and 60min each time, carrying out suction filtration after reflux extraction, and concentrating the filtrate at the temperature of 25 ℃ to obtain the rhubarb extract, wherein the relative density of the rhubarb is 1.20; the preparation method of the red peony root extract comprises the steps of taking red peony roots which are crushed and then screened by a 40-mesh sieve, adding 18 times of ethanol with the volume fraction of 80%, carrying out reflux extraction for 2 times and 60min each time at 55 ℃, carrying out suction filtration after reflux extraction, and concentrating filtrate at 25 ℃ to obtain the red peony root extract, wherein the relative density of the filtrate is 1.20; the preparation method of the achyranthes bidentata extract comprises the steps of taking achyranthes bidentata which are ground and sieved by a 40-mesh sieve, adding 75% ethanol in an amount which is 10 times of the mass volume fraction, carrying out reflux extraction for 2 times and 50min each time at 70 ℃, carrying out suction filtration after reflux extraction, and concentrating the filtrate to the relative density of 1.20 at 25 ℃ to obtain the achyranthes bidentata extract.
As a preferred scheme of the preparation method of the traditional Chinese medicine gout plaster, the preparation method comprises the following steps: the preparation method of the Poria extract comprises pulverizing Poria, sieving with 20 mesh sieve, adding 10 times of 70% ethanol, reflux-extracting at 70 deg.C for 2 times (each time for 50 min), suction-filtering, and concentrating the filtrate at 25 deg.C to relative density of 1.20 to obtain Poria extract; the preparation method of the Chinese violet extract comprises the steps of taking crushed Chinese violet, adding 75% ethanol in 10 times of mass volume fraction, leaching for 24 hours at 25 ℃, filtering to obtain a first filtrate, adding 80% ethanol in 6 times of mass volume fraction into filter residue, leaching for 12 hours at room temperature, filtering to obtain a second filtrate, adding 75% ethanol in 6 times of mass volume fraction into filter residue again, leaching for 8 hours at room temperature, filtering to obtain a third filtrate, combining the three filtrates, and concentrating the filtrate to the relative density of 1.20 at 25 ℃ to obtain the Chinese violet extract; the preparation method of the subprostrate sophora root extract comprises the steps of adding 85% ethanol in an amount which is 12 times the mass volume of the subprostrate sophora root after being crushed, carrying out reflux extraction for 30min at the temperature of 60 ℃, filtering, concentrating the filtrate at the temperature of 25 ℃ to the relative density of 1.20, and obtaining the subprostrate sophora root extract.
As a preferred scheme of the preparation method of the traditional Chinese medicine gout plaster, the preparation method comprises the following steps: and (3) carrying out high-speed stirring and ultrasonic treatment for 15min to obtain the gout plaster composition, wherein the high-speed stirring rotating speed is 5000rpm, the stirring time is 20-30 min, the ultrasonic frequency is 50kHz, and the ultrasonic temperature is 40-50 ℃.
As a preferred scheme of the preparation method of the traditional Chinese medicine gout plaster, the preparation method comprises the following steps: and putting the mixture into a water bath kettle at the temperature of 40-55 ℃ for water bath swelling for 8-10 h to serve as a phase b, wherein the water bath temperature is 45 ℃.
As a preferred scheme of the preparation method of the traditional Chinese medicine gout plaster, the preparation method comprises the following steps: in the gout plaster composition, by weight, the traditional Chinese medicine extract accounts for 8%, the sodium carboxymethylcellulose accounts for 4%, the polyvinylpyrrolidone-K30 accounts for 15%, the ethylparaben accounts for 0.6%, the alcohol accounts for 15%, the glycerol accounts for 18%, the polyethylene glycol 400 accounts for 1.5%, the kaolin accounts for 10%, the citric acid accounts for 2%, the aluminum hydroxide accounts for 4%, and the balance is water.
The invention further aims to overcome the defects in the prior art and provide the traditional Chinese medicine gout plaster.
In order to solve the technical problems, the invention provides the following technical scheme: the traditional Chinese medicine gout patch prepared by the preparation method of the traditional Chinese medicine gout patch according to any one of claims 1 to 7 comprises a gout patch composition, wherein in the gout patch composition, by weight percent, the traditional Chinese medicine extract accounts for 5-10%, the sodium carboxymethylcellulose accounts for 2-4%, the polyvinylpyrrolidone-K30 accounts for 15-20%, the ethyl paraben accounts for 0.4-0.8%, the alcohol accounts for 15-18%, the glycerol accounts for 18-20%, the polyethylene glycol 400 accounts for 1.5-2%, the kaolin accounts for 10-15%, the citric acid accounts for 2-3%, the aluminum hydroxide accounts for 4-5%, and the balance is water.
As a preferred scheme of the traditional Chinese medicine gout plaster, the invention comprises the following steps: the traditional Chinese medicine extract is prepared from a rheum officinale extract, a red peony root extract, a achyranthes bidentata extract, a poria cocos extract, a philippine violet herb extract and a subprostrate sophora root extract according to a mass ratio of 1-2: 2-3: 1-2: 2-3: 2-3: 1-2.
As a preferred scheme of the traditional Chinese medicine gout plaster, the invention comprises the following steps: in the gout plaster composition, by weight, the traditional Chinese medicine extract accounts for 8%, the sodium carboxymethylcellulose accounts for 4%, the polyvinylpyrrolidone-K30 accounts for 15%, the ethylparaben accounts for 0.6%, the alcohol accounts for 15%, the glycerol accounts for 18%, the polyethylene glycol 400 accounts for 1.5%, the kaolin accounts for 10%, the citric acid accounts for 2%, the aluminum hydroxide accounts for 4%, and the balance is water.
The invention has the beneficial effects that:
(1) aiming at the problems that most of the existing gout treatment medicines are oral medicines and have the first pass effect and toxic and side effects, the search and development of external preparations for treating gout, especially the traditional Chinese medicine, are traditional Chinese medicine strategies and ways for treating and preventing gout and related diseases. The invention provides a preparation method of a traditional Chinese medicine gout patch, and the novel external patch prepared by the preparation method has the advantages of accurate local administration dosage, stable blood concentration and timely pain relieving effect, small allergic stimulation, comfortable and convenient use and the like, can reduce adverse reaction of medicines, improve compliance of clinical treatment, and is convenient for patients to use.
(2) The invention provides a preparation method of a traditional Chinese medicine gout plaster, which extracts active ingredients from rhubarb, red paeony root, twotooth achyranthes root, Indian buead, Chinese violet and subprostrate sophora by a specific process, wherein, the rhubarb contains active ingredients such as anthraquinone, anthrone, stilbene, tannin, organic acid, polysaccharide and the like, the red peony root contains monoterpene and glycosides with pinane structure such as paeoniflorin, hydroxyl-paeony oxypaeoniflorin, benzoyl-paeoniflorin and the like, the achyranthes root mainly contains sterone, saponin and polysaccharide compounds, the tuckahoe contains triterpenes and polysaccharide compounds, the Chinese violet contains flavone and coumarin compounds, the subprostrate sophora contains active ingredients such as alkaloid, flavone, triterpene and triterpenoid saponin and the like, and preferably selecting a rhubarb extract, a red peony extract, a achyranthes root extract, a poria cocos extract, a Chinese violet extract and a subprostrate sophora extract according to a mass ratio of 1-2: 2-3: 1-2: 2-3: 2-3: 1-2 to prepare the traditional Chinese medicine extract, and the active ingredients in the extracts have synergistic effect and have better curative effect on gout.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive exercise. Wherein:
fig. 1 is a traditional Chinese medicine gout patch prepared in embodiment 2 of the invention.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention more comprehensible, specific embodiments thereof are described in detail below with reference to examples of the specification.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described and will be readily apparent to those of ordinary skill in the art without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.
Furthermore, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one implementation of the invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.
The preparation method of the rhubarb extract comprises the following steps: pulverizing radix et rhizoma Rhei, sieving with 20 mesh sieve, adding 20 times of 70% ethanol, reflux extracting at 60 deg.C for 60min for 2 times, vacuum filtering, and concentrating the filtrate at 25 deg.C to relative density of 1.20 to obtain radix et rhizoma Rhei extract.
The preparation method of the red peony root extract comprises the following steps: collecting pulverized radix Paeoniae Rubra, sieving with 40 mesh sieve, adding 18 times of 80% ethanol, reflux-extracting at 55 deg.C for 2 times (60 min each time), suction-filtering, and concentrating the filtrate at 25 deg.C to relative density of 1.20 to obtain radix Paeoniae Rubra extract.
The preparation method of the achyranthes bidentata extract comprises the following steps: taking the radix achyranthis bidentatae which is crushed and sieved by a 40-mesh sieve, adding ethanol with the mass volume fraction of 10 times of 75%, carrying out reflux extraction for 2 times at 70 ℃ for 50min each time, carrying out suction filtration after reflux extraction, and concentrating the filtrate at 25 ℃ to obtain the radix achyranthis bidentatae extract, wherein the relative density of the filtrate is 1.20.
The preparation method of the poria extract comprises the following steps: pulverizing Poria, sieving with 20 mesh sieve, adding 10 times of 70% ethanol, reflux-extracting at 70 deg.C for 50min for 2 times, suction-filtering, and concentrating the filtrate at 25 deg.C to relative density of 1.20 to obtain Poria extract.
The preparation method of the Chinese violet extract comprises the following steps: taking crushed Chinese violet, adding 10 times of ethanol with the mass volume fraction of 75%, leaching for 24h at 25 ℃, filtering to obtain a first filtrate, adding 6 times of ethanol with the mass volume fraction of 80% into filter residue, leaching for 12h at room temperature, filtering to obtain a second filtrate, adding 6 times of ethanol with the mass volume fraction of 75% into the filter residue again, leaching for 8h at room temperature, filtering to obtain a third filtrate, combining the third filtrates, and concentrating the filtrate to the relative density of 1.20 at 25 ℃ to obtain the Chinese violet extract.
The preparation method of the subprostrate sophora extract comprises the following steps: adding 85% ethanol 12 times the weight volume of the pulverized radix Sophorae Tonkinensis, reflux extracting at 60 deg.C for 30min, filtering, and concentrating the filtrate at 25 deg.C to relative density of 1.20 to obtain radix Sophorae Tonkinensis extract.
Example 1
The embodiment provides a traditional Chinese medicine gout plaster and a preparation method thereof:
(1) the traditional Chinese medicine gout plaster comprises the following components: the traditional Chinese medicine composition comprises traditional Chinese medicine extracts, sodium carboxymethylcellulose, polyvinylpyrrolidone-K30, ethylparaben, alcohol, glycerol, polyethylene glycol 400, kaolin, citric acid, aluminum hydroxide and water, wherein the traditional Chinese medicine extracts comprise rheum officinale extracts, red paeony root extracts, achyranthes bidentata extracts, poria cocos extracts, Chinese violet extracts and subprostrate sophora extracts in a mass ratio of 1: 2: 1: 2: 2: 1, mixing;
by weight percentage of the gout plaster composition, the traditional Chinese medicine extract is 5%, the sodium carboxymethylcellulose is 4%, the polyvinylpyrrolidone-K30 is 20%, the ethylparaben is 0.4%, the alcohol is 15%, the glycerol is 18%, the polyethylene glycol 400 is 1.5%, the kaolin is 10%, the citric acid is 2%, the aluminum hydroxide is 4%, and the balance is water.
(2) Preparing the traditional Chinese medicine gout plaster according to the formula of the traditional Chinese medicine gout plaster in the step (1):
taking a rhubarb extract, a red peony root extract, a achyranthes root extract, a poria cocos extract, a Chinese violet extract and a subprostrate sophora extract according to a mass ratio of 1: 2: 1: 2: 2: 1, mixing to obtain a traditional Chinese medicine extract;
adding sodium carboxymethylcellulose into 10 times of water, cold swelling for 10h, swelling in 50 deg.C water bath for 6h, and stirring to obtain a uniform transparent phase a;
mixing polyvinylpyrrolidone-K30, ethylparaben, 30% alcohol by volume fraction, and Chinese medicinal extract, and swelling in water bath at 40 deg.C for 8 hr to obtain phase b;
uniformly stirring glycerol, polyethylene glycol 400 and kaolin until no granular feeling exists, and taking the mixture as a phase c;
mixing citric acid and aluminum hydroxide to obtain a d phase;
adding phase d into phase a, stirring thoroughly, adding phase c, adding phase b, stirring at 5000rpm for 20min, ultrasonic treating at 40 deg.C and ultrasonic frequency of 50kHz for 15min to obtain gout plaster composition;
coating the gout plaster composition, placing in a 40 ℃ electric heating constant temperature blast drying oven for drying for 8h, taking out, slightly cooling, and covering with a mucous membrane to obtain the traditional Chinese medicine gout plaster.
Example 2
The embodiment provides a traditional Chinese medicine gout plaster and a preparation method thereof:
(1) the traditional Chinese medicine gout plaster comprises the following components: the traditional Chinese medicine composition comprises traditional Chinese medicine extracts, sodium carboxymethylcellulose, polyvinylpyrrolidone-K30, ethylparaben, alcohol, glycerol, polyethylene glycol 400, kaolin, citric acid, aluminum hydroxide and water, wherein the traditional Chinese medicine extracts comprise rheum officinale extracts, red paeony root extracts, achyranthes bidentata extracts, poria cocos extracts, Chinese violet extracts and subprostrate sophora extracts in a mass ratio of 2: 3: 1: 3: 3: 2, mixing;
in the gout plaster composition, by weight, the traditional Chinese medicine extract accounts for 8%, the sodium carboxymethylcellulose accounts for 4%, the polyvinylpyrrolidone-K30 accounts for 15%, the ethylparaben accounts for 0.6%, the alcohol accounts for 15%, the glycerol accounts for 18%, the polyethylene glycol 400 accounts for 1.5%, the kaolin accounts for 10%, the citric acid accounts for 2%, the aluminum hydroxide accounts for 4%, and the balance is water.
(2) Preparing the traditional Chinese medicine gout plaster according to the formula of the traditional Chinese medicine gout plaster in the step (1):
taking a rhubarb extract, a red peony root extract, a achyranthes root extract, a poria cocos extract, a Chinese violet extract and a subprostrate sophora extract according to a mass ratio of 2: 3: 1: 3: 3: 2 mixing to obtain Chinese medicinal extract;
adding sodium carboxymethylcellulose into 10 times of water, cold swelling for 10h, swelling in 50 deg.C water bath for 6h, and stirring to obtain a uniform transparent phase a;
mixing polyvinylpyrrolidone-K30, ethylparaben, 30% alcohol by volume fraction, and Chinese medicinal extract, and swelling in water bath at 40 deg.C for 8 hr to obtain phase b;
uniformly stirring glycerol, polyethylene glycol 400 and kaolin until no granular feeling exists, and taking the mixture as a phase c;
mixing citric acid and aluminum hydroxide to obtain a d phase;
adding phase d into phase a, stirring thoroughly, adding phase c, adding phase b, stirring at 5000rpm for 20min, ultrasonic treating at 40 deg.C and ultrasonic frequency of 50kHz for 15min to obtain gout plaster composition;
coating the gout plaster composition, drying in 55 deg.C electric heating constant temperature blast drying oven for 8 hr, taking out, cooling, and covering with anti-mucosa to obtain Chinese medicinal gout plaster shown in figure 1.
Example 3
The embodiment provides a traditional Chinese medicine gout plaster and a preparation method thereof:
(1) the traditional Chinese medicine gout plaster comprises the following components: the traditional Chinese medicine composition comprises traditional Chinese medicine extracts, sodium carboxymethylcellulose, polyvinylpyrrolidone-K30, ethylparaben, alcohol, glycerol, polyethylene glycol 400, kaolin, citric acid, aluminum hydroxide and water, wherein the traditional Chinese medicine extracts comprise rheum officinale extracts, red paeony root extracts, achyranthes bidentata extracts, poria cocos extracts, Chinese violet extracts and subprostrate sophora extracts in a mass ratio of 2: 3: 2: 3: 3: 1, mixing;
by weight percentage of the gout plaster composition, the traditional Chinese medicine extract is 6%, the sodium carboxymethylcellulose is 2%, the polyvinylpyrrolidone-K30 is 20%, the ethylparaben is 0.4%, the alcohol is 15%, the glycerol is 20%, the polyethylene glycol 400 is 2%, the kaolin is 15%, the citric acid is 3%, the aluminum hydroxide is 5%, and the balance is water.
(2) Preparing the traditional Chinese medicine gout plaster according to the formula of the traditional Chinese medicine gout plaster in the step (1):
taking a rhubarb extract, a red peony root extract, a achyranthes root extract, a poria cocos extract, a Chinese violet extract and a subprostrate sophora extract according to a mass ratio of 2: 3: 2: 3: 3: 1, mixing to obtain a traditional Chinese medicine extract;
adding sodium carboxymethylcellulose into 12 times of water, cold swelling for 10h, swelling in 55 deg.C water bath for 8h, and stirring to obtain a uniform transparent phase a;
mixing polyvinylpyrrolidone-K30, ethylparaben, 40% ethanol by volume fraction, and Chinese medicinal extract, and swelling in 55 deg.C water bath for 10 hr to obtain phase b;
uniformly stirring glycerol, polyethylene glycol 400 and kaolin until no granular feeling exists, and taking the mixture as a phase c;
mixing citric acid and aluminum hydroxide to obtain a d phase;
adding phase d into phase a, stirring thoroughly, adding phase c, adding phase b, stirring at 5000rpm for 30min, ultrasonic treating at 50 deg.C and ultrasonic frequency of 50kHz for 15min to obtain gout plaster composition;
coating the gout plaster composition, placing in an electric heating constant temperature blast drying oven at 55 ℃ for drying for 8h, taking out, slightly cooling, and covering with a mucous membrane to obtain the traditional Chinese medicine gout plaster.
Clinical data: the invention takes gout in traditional Chinese medicine as an example, and selects 120 cases for clinical observation.
The traditional Chinese medicine gout diagnosis is selected as a standard: patients with hyperuricemia often have severe pain in joints at midnight, usually red, swollen, hot and painful joints, effusion in joint cavities and painful stone precipitation.
In selected cases, 70 men and 50 women had no other serious diseases, and the age was between 40 and 55 years, with the average age being 46.5 years.
In clinical trial, other medicines and health care nutriments are not used.
The treatment method comprises the following steps: the traditional Chinese medicine gout plaster prepared in the embodiment 1-3 is applied to the affected part, and the gout plaster is changed once a day.
Control group: the oral liquid for gout treatment of the control group is taken 3 times a day, and the dosage is 10mL each time.
The curative effect index is as follows: local symptoms, signs.
The therapeutic effect judgment standard is as follows:
the effect is shown: after the treatment, the symptoms of pain and red swelling of the joint of the patient disappear.
The method has the following advantages: after finger treatment, the pain and redness of the joint of the patient are relieved.
And (4) invalidation: means that after treatment, the clinical symptoms of the patient are not improving or are aggravating.
The total effective rate of the treatment is (effective + effective) cases/total cases multiplied by 100 percent
The total therapeutic effect of examples 1 to 3 and the control group is shown in Table 1.
TABLE 1
| Total number (human) | Display effect (human) | Effective (human) | Invalid (human) | |
| Example 1 | 30 | 22 | 7 | 1 |
| Example 2 | 30 | 24 | 5 | 1 |
| Example 3 | 30 | 23 | 6 | 1 |
| Control group | 30 | 15 | 13 | 2 |
Example 4
(1) The traditional Chinese medicine gout plaster has the influence on the microcrystalline sodium urate-induced rat foot swelling, and the volume difference of feet 4h after inflammation is calculated, so that the swelling inhibition rate is calculated:
swelling inhibition ratio (%) (difference in volume of blank matrix foot-difference in volume of foot after traditional Chinese medicine gout patch action) ÷ difference in volume of blank matrix foot
On the basis of example 2, the formulas of the traditional Chinese medicine extracts (the mass ratio of the extracts) are different, other conditions are unchanged, the influence of the traditional Chinese medicine gout patch on the microcrystalline sodium urate-induced rat foot swelling is calculated, and the swelling inhibition rate is calculated, and the result is shown in table 2.
TABLE 2
As can be seen from Table 2, when no extract of radix et rhizoma Rhei, radix Paeoniae Rubra, Achyranthis radix, Poria, herba Violae and radix Sophorae Tonkinensis is added, the effect of gout plaster for inhibiting mouse swelling for 4 hr is reduced, and may be caused by the fact that radix et rhizoma Rhei contains active ingredients such as anthraquinone, anthrone, stilbene, tannin, organic acid and polysaccharide, radix Paeoniae Rubra contains monoterpene with pinane structure and its glycosides such as paeoniflorin, hydroxy Paeoniflorin, benzoyl paeoniflorin, Achyranthis radix mainly contains sterone, saponin and polysaccharide, Poria contains triterpene and polysaccharide, herba Violae contains flavone and coumarin compounds, radix Sophorae Tonkinensis contains active ingredients such as alkaloid, flavone, triterpene and triterpene saponin, and preferably radix et rhizoma Rhei, radix Paeoniae Rubra and Achyranthis radix extract, The weight ratio of the poria cocos extract to the viola yedoensis makino extract to the subprostrate sophora subprostrata extract is 1-2: 2-3: 1-2: 2-3: 2-3: 1-2 to prepare the traditional Chinese medicine extract, and the active ingredients in the extracts have synergistic effect to achieve the best curative effect on the swelling inhibition rate.
(2) On the basis of the example 2, different traditional Chinese medicine extracts are taken, other conditions are unchanged, the influence of the traditional Chinese medicine gout plaster on the microcrystalline sodium urate-induced rat foot swelling is calculated, and the swelling inhibition rate is calculated, and the result is shown in table 3.
TABLE 3
Note: the cortex phellodendri extract, the radix paeoniae rubra extract, the cortex moutan extract and the radix gentianae macrophyllae extract are all ethanol extracts, the crushed traditional Chinese medicine raw materials are sieved by a 40-mesh sieve, 10 times of ethanol with the mass volume fraction of 75% is added, reflux extraction is carried out for 2 times at the temperature of 30 ℃, each time is 60min, suction filtration is carried out after reflux extraction, and the filtrate is concentrated to the relative density of 1.20 at the temperature of 25 ℃, so that the extracts of the active ingredients are obtained.
As can be seen from Table 3, the raw materials of the present invention were replaced with other raw materials, and the inhibition rate of swelling was decreased, and the final results were different due to different active ingredients in different Chinese medicinal materials and different effects between the active ingredients. According to the invention, extracts of rhubarb, red peony root, achyranthes root, tuckahoe, Chinese violet and subprostrate sophora are preferably selected as active ingredients of the traditional Chinese medicine gout plaster, and the active ingredients in the extracts have synergistic effect, so that the traditional Chinese medicine gout plaster has a better curative effect on gout.
Example 5
On the basis of example 2, the preparation methods of the rhubarb extract, the red peony extract, the achyranthes root extract, the tuckahoe extract, the Chinese violet extract and the subprostrate sophora extract are replaced by the following steps: respectively taking the traditional Chinese medicine raw materials, adding water with the weight of 10 times of the medicinal materials, decocting for 2 times, each time for 1 hour, filtering, combining the filtrates, and concentrating the filtrate at 25 ℃ to obtain a clear paste with the relative density of 1.20.
And (3) calculating the influence of the traditional Chinese medicine gout plaster on the microcrystalline sodium urate-induced rat foot swelling and calculating the swelling inhibition rate under the condition that other conditions are unchanged, wherein the results are shown in a table 4.
TABLE 4
| Example 2 | Example 5 | |
| 4h swelling inhibition (%) | 46 | 32 |
As can be seen from table 4, different herb extracts have different active ingredients in the final herb extract due to different extraction methods, resulting in a large difference in the final swelling inhibition effect.
Example 6
The embodiment provides a traditional Chinese medicine gout plaster and a preparation method thereof:
(1) the traditional Chinese medicine gout plaster comprises the following components: the traditional Chinese medicine composition comprises traditional Chinese medicine extracts, sodium carboxymethylcellulose, polyvinylpyrrolidone-K30, ethylparaben, alcohol, glycerol, polyethylene glycol 400, kaolin, citric acid, aluminum hydroxide and water, wherein the traditional Chinese medicine extracts comprise rheum officinale extracts, red paeony root extracts, achyranthes bidentata extracts, poria cocos extracts, Chinese violet extracts and subprostrate sophora extracts in a mass ratio of 2: 3: 1: 3: 3: 2, mixing;
in the gout plaster composition, by weight, the traditional Chinese medicine extract accounts for 8%, the sodium carboxymethylcellulose accounts for 4%, the polyvinylpyrrolidone-K30 accounts for 15%, the ethylparaben accounts for 0.6%, the alcohol accounts for 15%, the glycerol accounts for 18%, the polyethylene glycol 400 accounts for 1.5%, the kaolin accounts for 10%, the citric acid accounts for 2%, the aluminum hydroxide accounts for 4%, and the balance is water.
(2) Preparing the traditional Chinese medicine gout plaster according to the formula of the traditional Chinese medicine gout plaster in the step (1):
taking a rhubarb extract, a red peony root extract, a achyranthes root extract, a poria cocos extract, a Chinese violet extract and a subprostrate sophora extract according to a mass ratio of 2: 3: 1: 3: 3: 2 mixing to obtain Chinese medicinal extract;
adding sodium carboxymethylcellulose into 10 times of water, cold swelling for 10h, swelling in 50 deg.C water bath for 6h, and stirring to obtain a uniform transparent phase a;
mixing polyvinylpyrrolidone-K30, ethylparaben, 30% alcohol by volume fraction, and Chinese medicinal extract, and swelling in water bath at 40 deg.C for 8 hr to obtain phase b;
uniformly stirring glycerol, polyethylene glycol 400 and kaolin until no granular feeling exists, and taking the mixture as a phase c;
mixing citric acid and aluminum hydroxide to obtain a d phase;
adding phase c into phase a, stirring thoroughly, adding phase d, adding phase b, stirring at 5000rpm for 20min, ultrasonic treating at 40 deg.C and ultrasonic frequency of 50kHz for 15min to obtain gout plaster composition;
coating the gout plaster composition, placing in an electric heating constant temperature blast drying oven at 55 ℃ for drying for 8h, taking out, slightly cooling, and covering with a mucous membrane to obtain the traditional Chinese medicine gout plaster.
The influence of the traditional Chinese medicine gout plaster on the microcrystalline sodium urate-induced rat foot swelling is calculated, the swelling inhibition rate is calculated, and the result is shown in table 5.
TABLE 5
| Example 2 | Example 6 | |
| 4h swelling inhibition (%) | 46 | 38 |
As can be seen from table 5, in the preparation method of the traditional Chinese medicine gout plaster of the present invention, phase d needs to be added into phase a, after fully and uniformly stirring, phase c is added, phase b is added, high speed stirring is performed, and after ultrasonic treatment is performed for 15min, the gout plaster composition is obtained. Experiments prove that only by the preparation method and the feeding sequence, the obtained traditional Chinese medicine gout plaster can obtain better gout treatment curative effect, which shows that the preparation method and the feeding sequence also play a decisive role in the properties of finished gout plaster products.
The main chemical components of the rhubarb in the invention are anthraquinone, anthrone, stilbenes, tannin, organic acid, polysaccharide and the like. Pharmacological research proves that the water and alcohol extracts of rhubarb have pharmacological effects in vitro including antibiosis, anti-inflammation, anti-tumor, immunoregulation and the like.
The main chemical components of red peony root in the invention are monoterpene and its glycosides, and the monoterpene and its glycosides with pinane structure include paeoniflorin, hydroxy-paeoniflorin, benzoylpaeoniflorin, etc. Pharmacological research proves that the water and alcohol extracts of red peony root have pharmacological actions in vitro including analgesia, antibiosis, anti-inflammation and the like.
The achyranthes bidentata in the invention mainly contains sterone, saponin and polysaccharide compounds. The triterpene saponin component can inhibit osteoclast formation, thereby playing the role of resisting osteoporosis, especially the highest activity of the glucuronide of oleanolic acid; the total saponins of achyranthes have obvious effects of resisting inflammation, relieving pain and promoting blood circulation, and have good treatment effect on gonarthritis.
The tuckahoe mainly contains two compounds of triterpenoid box polysaccharide. The total triterpenes component is one of the effective parts with the anti-inflammatory effect of the tuckahoe, can resist acute and chronic inflammation under different experimental models, and has high remarkable anti-inflammatory effect which is recognized in foreign countries.
The main chemical components of the Chinese violet in the invention are flavone and coumarin compounds. Pharmacological research proves that the aqueous and alcohol extracts of the Chinese violet have wide pharmacological effects, and particularly have obvious effects such as anti-inflammatory effect.
The subprostrate sophora in the invention contains various chemical components such as alkaloid, flavone, triterpene and triterpene saponin. The subprostrate sophora aqueous solution has good anti-inflammatory effect.
The invention provides a preparation method of a traditional Chinese medicine gout plaster, which extracts active ingredients from rhubarb, red paeony root, twotooth achyranthes root, Indian buead, Chinese violet and subprostrate sophora by a specific process, wherein, the rhubarb contains active ingredients such as anthraquinone, anthrone, stilbene, tannin, organic acid, polysaccharide and the like, the red peony root contains monoterpene and glycosides with pinane structure such as paeoniflorin, hydroxyl-paeony oxypaeoniflorin, benzoyl-paeoniflorin and the like, the achyranthes root mainly contains sterone, saponin and polysaccharide compounds, the tuckahoe contains triterpenes and polysaccharide compounds, the Chinese violet contains flavone and coumarin compounds, the subprostrate sophora contains active ingredients such as alkaloid, flavone, triterpene and triterpenoid saponin and the like, selecting a rhubarb extract, a red peony root extract, a achyranthes root extract, a poria cocos extract, a Chinese violet extract and a subprostrate sophora extract according to a mass ratio of 1-2: 2-3: 1-2: 2-3: 2-3: 1-2 to prepare the traditional Chinese medicine extract, and the active ingredients in the extracts have synergistic effect and have better curative effect on gout.
It should be noted that the above-mentioned embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the claims of the present invention.
Claims (8)
1. A preparation method of a traditional Chinese medicine gout plaster is characterized by comprising the following steps: comprises the steps of (a) preparing a mixture of a plurality of raw materials,
taking a rhubarb extract, a red peony root extract, a achyranthes root extract, a poria cocos extract, a Chinese violet extract and a subprostrate sophora extract according to a mass ratio of 1-2: 2-3: 1-2: 2-3: 2-3: 1-2, mixing to obtain a traditional Chinese medicine extract;
adding sodium carboxymethylcellulose into water with the mass of 10-12 times of that of the sodium carboxymethylcellulose, performing cold expansion for 10-12 hours, putting the sodium carboxymethylcellulose into a water bath kettle with the temperature of 50-55 ℃ for water bath swelling for 6-8 hours, and stirring the mixture until the mixture is uniform and transparent to be used as a phase a;
mixing polyvinylpyrrolidone-K30, ethylparaben, 30-40% alcohol by volume fraction and the traditional Chinese medicine extract, and putting the mixture into a water bath kettle at the temperature of 40-55 ℃ for swelling for 8-10 hours to serve as a phase b;
uniformly stirring glycerol, polyethylene glycol 400 and kaolin until no granular feeling exists, and taking the mixture as a phase c;
mixing citric acid and aluminum hydroxide to obtain a d phase;
adding the phase d into the phase a, fully and uniformly stirring, adding the phase c, then adding the phase b, stirring at a high speed, and carrying out ultrasonic treatment for 15min to obtain a gout plaster composition;
coating the gout plaster composition, placing the gout plaster composition in an electric heating constant-temperature blast drying oven at 40-55 ℃ for drying for 8h, taking out the gout plaster composition, slightly cooling the gout plaster composition, and covering an anti-mucous membrane to obtain the traditional Chinese medicine gout plaster;
in the gout plaster composition, by weight, the traditional Chinese medicine extract accounts for 5-10%, the sodium carboxymethylcellulose accounts for 2-4%, the polyvinylpyrrolidone-K30 accounts for 15-20%, the ethyl hydroxybenzoate accounts for 0.4-0.8%, the alcohol accounts for 15-18%, the glycerol accounts for 18-20%, the polyethylene glycol 400 accounts for 1.5-2%, the kaolin accounts for 10-15%, the citric acid accounts for 2-3%, the aluminum hydroxide accounts for 4-5%, and the balance is water;
the preparation method of the rhubarb extract comprises the steps of taking rhubarb which is ground and sieved by a 20-mesh sieve, adding ethanol with the mass volume fraction of 70 percent of 20 times, carrying out reflux extraction for 2 times at the temperature of 60 ℃ and 60min each time, carrying out suction filtration after reflux extraction, and concentrating the filtrate at the temperature of 25 ℃ to obtain the rhubarb extract, wherein the relative density of the rhubarb is 1.20;
the preparation method of the red peony root extract comprises the steps of taking red peony roots which are crushed and then screened by a 40-mesh sieve, adding 18 times of ethanol with the volume fraction of 80%, carrying out reflux extraction for 2 times and 60min each time at 55 ℃, carrying out suction filtration after reflux extraction, and concentrating filtrate at 25 ℃ to obtain the red peony root extract, wherein the relative density of the filtrate is 1.20;
the preparation method of the achyranthes bidentata extract comprises the steps of taking achyranthes bidentata which are crushed and sieved by a 40-mesh sieve, adding 75% ethanol in an amount which is 10 times of the mass volume fraction, performing reflux extraction for 2 times at 70 ℃ for 50min each time, performing suction filtration after reflux extraction, and concentrating filtrate at 25 ℃ to obtain the achyranthes bidentata extract, wherein the relative density of the filtrate is 1.20;
the preparation method of the Poria extract comprises pulverizing Poria, sieving with 20 mesh sieve, adding 10 times of 70% ethanol, reflux-extracting at 70 deg.C for 2 times (each time for 50 min), suction-filtering, and concentrating the filtrate at 25 deg.C to relative density of 1.20 to obtain Poria extract;
the preparation method of the Chinese violet extract comprises the steps of taking crushed Chinese violet, adding 75% ethanol in 10 times of mass volume fraction, leaching for 24 hours at 25 ℃, filtering to obtain a first filtrate, adding 80% ethanol in 6 times of mass volume fraction into filter residue, leaching for 12 hours at room temperature, filtering to obtain a second filtrate, adding 75% ethanol in 6 times of mass volume fraction into filter residue again, leaching for 8 hours at room temperature, filtering to obtain a third filtrate, combining the three filtrates, and concentrating the filtrate to the relative density of 1.20 at 25 ℃ to obtain the Chinese violet extract;
the preparation method of the subprostrate sophora root extract comprises the steps of adding 85% ethanol in an amount which is 12 times the mass volume of the subprostrate sophora root after being crushed, carrying out reflux extraction for 30min at the temperature of 60 ℃, filtering, concentrating the filtrate at the temperature of 25 ℃ to the relative density of 1.20, and obtaining the subprostrate sophora root extract.
2. The preparation method of the traditional Chinese medicine gout plaster of claim 1, which is characterized in that: the rheum officinale extract, the red paeony root extract, the achyranthes bidentata extract, the poria cocos extract, the Chinese violet extract and the subprostrate sophora extract are mixed according to the mass ratio of 2: 3: 1: 3: 3: 2.
3. the preparation method of the traditional Chinese medicine gout plaster of claim 1, which is characterized in that: and (3) carrying out high-speed stirring and ultrasonic treatment for 15min to obtain the gout plaster composition, wherein the high-speed stirring rotating speed is 5000rpm, the stirring time is 20-30 min, the ultrasonic frequency is 50kHz, and the ultrasonic temperature is 40-50 ℃.
4. The preparation method of the traditional Chinese medicine gout plaster of claim 1, which is characterized in that: and putting the mixture into a water bath kettle at the temperature of 40-55 ℃ for water bath swelling for 8-10 h to serve as a phase b, wherein the water bath temperature is 45 ℃.
5. The preparation method of the traditional Chinese medicine gout plaster of claim 1, which is characterized in that: in the gout plaster composition, by weight, the traditional Chinese medicine extract accounts for 8%, the sodium carboxymethylcellulose accounts for 4%, the polyvinylpyrrolidone-K30 accounts for 15%, the ethylparaben accounts for 0.6%, the alcohol accounts for 15%, the glycerol accounts for 18%, the polyethylene glycol 400 accounts for 1.5%, the kaolin accounts for 10%, the citric acid accounts for 2%, the aluminum hydroxide accounts for 4%, and the balance is water.
6. The traditional Chinese medicine gout plaster prepared by the preparation method of the traditional Chinese medicine gout plaster according to any one of claims 1 to 5, and is characterized in that: the patch composition comprises, by weight, 5-10% of a traditional Chinese medicine extract, 2-4% of sodium carboxymethylcellulose, 15-20% of polyvinylpyrrolidone-K30, 0.4-0.8% of ethyl paraben, 15-18% of alcohol, 18-20% of glycerol, 1.5-2% of polyethylene glycol 400, 10-15% of kaolin, 2-3% of citric acid, 4-5% of aluminum hydroxide and the balance water.
7. The traditional Chinese medicine gout plaster prepared by the preparation method of the traditional Chinese medicine gout plaster according to any one of claims 1 to 5, and is characterized in that: the traditional Chinese medicine extract is prepared from a rheum officinale extract, a red peony root extract, a achyranthes bidentata extract, a poria cocos extract, a philippine violet herb extract and a subprostrate sophora root extract according to a mass ratio of 1-2: 2-3: 1-2: 2-3: 2-3: 1-2.
8. The traditional Chinese medicine gout plaster prepared by the preparation method of the traditional Chinese medicine gout plaster according to any one of claims 1 to 5, and is characterized in that: in the gout plaster composition, by weight, the traditional Chinese medicine extract accounts for 8%, the sodium carboxymethylcellulose accounts for 4%, the polyvinylpyrrolidone-K30 accounts for 15%, the ethylparaben accounts for 0.6%, the alcohol accounts for 15%, the glycerol accounts for 18%, the polyethylene glycol 400 accounts for 1.5%, the kaolin accounts for 10%, the citric acid accounts for 2%, the aluminum hydroxide accounts for 4%, and the balance is water.
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| CN101590168A (en) * | 2008-05-27 | 2009-12-02 | 北京因科瑞斯医药科技有限公司 | A kind of Chinese medicine composition that is used for the treatment of gout and preparation method thereof |
| CN108904614A (en) * | 2018-09-19 | 2018-11-30 | 陈小朵 | The Chinese medicine for treating gout and legs and feet pain |
| CN110051804A (en) * | 2019-05-20 | 2019-07-26 | 贵州灵康士生物科技有限公司 | A kind of composition improving gout |
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| CN100531769C (en) * | 2006-09-05 | 2009-08-26 | 广州固志医药科技有限公司 | Chinese medicine composition for treating gout and preparing method thereof |
| CN108721414A (en) * | 2017-04-13 | 2018-11-02 | 上海寿叶生物科技有限公司 | Treat the cataplasm and preparation method thereof of gout |
| CN110354222A (en) * | 2019-08-20 | 2019-10-22 | 江苏博润医疗集团有限公司 | It is a kind of for treating the external drug of gout |
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| CN101590168A (en) * | 2008-05-27 | 2009-12-02 | 北京因科瑞斯医药科技有限公司 | A kind of Chinese medicine composition that is used for the treatment of gout and preparation method thereof |
| CN108904614A (en) * | 2018-09-19 | 2018-11-30 | 陈小朵 | The Chinese medicine for treating gout and legs and feet pain |
| CN110051804A (en) * | 2019-05-20 | 2019-07-26 | 贵州灵康士生物科技有限公司 | A kind of composition improving gout |
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