CN111474293B - Method and system for determining bacterial wilt solution - Google Patents
Method and system for determining bacterial wilt solution Download PDFInfo
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Abstract
The invention discloses a method and a system for determining a bacterial wilt solution, wherein the method comprises the following steps: acquiring a titration signal to obtain titration information, and establishing a titration rate curve according to the titration information; solution titration is carried out according to titration information, meanwhile, flow information of a test solution is detected, and absorption information of the test strip solution is obtained; acquiring solution temperature, determining solution temperature control information, obtaining temperature control interference information, and acquiring correction information by using a trend removal algorithm through the temperature control interference information; determining a measurement mode according to the solution absorption information and the correction information to obtain measurement information; and synchronously displaying the measurement information.
Description
Technical Field
The invention relates to the field of germ detection and solution measurement, in particular to a bacterial wilt solution measurement method and a bacterial wilt solution measurement system.
Background
The tobacco bacterial wilt is a vascular bundle disease caused by Lawsonia of Solanaceae, is difficult to detect at the initial stage, has no effect on drug control once wilting symptoms exist, strengthens detection of undeveloped tobacco roots of the bacteria, is critical for advanced control, and researches show that the incidence of tobacco plants rises along with the increase of the quantity of bacterial wilt in the tobacco roots and the foot leaves.
The traditional bacterial wilt solution measurement is carried out through a fluorescence detection method, quantitative test cannot be carried out in the measurement process, the test effect is poor, in order to accurately measure the bacterial wilt solution, a system matched with the bacterial wilt solution needs to be developed for control, the bacterial wilt solution is accurately measured through the system, and the measurement information is corrected through solution absorption information, so that the bacterial wilt solution is accurately measured, but in the control process, how to accurately control and how to realize the rapidity of data transmission are all the problems which cannot be solved.
Disclosure of Invention
The invention overcomes the defects of the prior art and provides a method and a system for measuring a bacterial wilt solution. A method for determining a bacterial wilt solution comprises the following steps: a first sensor and a second sensor,
the first sensor is arranged on the test strip, monitors titration signals of the bacterial wilt bacteria solution through the first sensor to obtain instantaneous titration information, and establishes a titration rate curve according to the titration information;
the plurality of test solution channels are arranged on the test strip at intervals, solution titration is carried out according to titration information, the titrated solution flows in the test solution channels, meanwhile, the flow information of the test solution in the test solution channels is detected, and the absorption information of the test strip solution is obtained;
monitoring the temperature of the solution of the bacterial wilt bacteria through a second sensor, determining solution temperature control information, obtaining temperature control interference information, and obtaining correction information through a trend removal algorithm through the temperature control interference information;
establishing a titration mathematical model according to Matlab to obtain initial titration information, establishing a database, and extracting solution absorption information through data index to obtain corresponding corrected titration information;
obtaining titration deviation information by correcting titration information to obtain titration deviation rate, and judging whether the titration deviation rate is larger than a preset deviation rate threshold value;
if the deviation rate is larger than the preset deviation rate threshold value, performing reverse correction and adjustment of the corrected titration information;
and determining a measurement mode according to the solution absorption information and the correction information to obtain measurement information, and synchronously displaying the measurement information through a display.
In a preferred embodiment of the invention, a titration signal is obtained to obtain titration information, and an initial titration rate curve is established according to the titration information; the method specifically comprises the following steps:
acquiring a titration signal, acquiring the information of a test solution channel, and establishing a titration mathematical model;
obtaining initial titration information according to a titration mathematical model;
analyzing initial titration information to obtain initial titration rate;
a titration rate curve is established based on the initial titration rate.
In a preferred embodiment of the invention, solution titration is carried out according to titration information, and meanwhile, solution absorption signals on the test paper strip are detected, so that solution absorption information is obtained, and a solution absorption rate curve is established; the method comprises the following steps:
detecting the characteristics of the test strip, acquiring the absorption characteristics of the test strip, and establishing a test strip absorption information database;
screening database information, and removing bad points and deviation data of the data;
extracting effective information of a database, and analyzing and obtaining solution absorption information;
establishing an information model according to the solution absorption information;
and analyzing the information model to obtain a solution absorption rate curve.
In a preferred embodiment of the present invention, corrected titration information is obtained according to the solution absorption information and the solution absorption rate curve; the method specifically comprises the following steps:
performing curve segmentation extraction according to the solution absorption rate curve to obtain extraction information;
according to the extracted information, discrete distribution nodes are established, and drift nodes are removed by using a trending removal algorithm;
extracting curve space state parameters and establishing a database;
and extracting solution absorption information through data index to obtain corresponding corrected titration information.
In a preferred embodiment of the present invention, the titration rate is corrected according to the corrected titration information; the method specifically comprises the following steps:
obtaining initial titration information according to a titration mathematical model;
extracting solution absorption information through data index to obtain corresponding corrected titration information;
obtaining titration deviation information by correcting titration information to obtain titration deviation rate;
judging whether the titration deviation rate is larger than a preset deviation rate threshold value or not;
and if the deviation rate is larger than the preset deviation rate threshold value, performing reverse correction adjustment for correcting the titration information.
In a preferred embodiment of the present invention, the database is divided into a plurality of sub-databases, each sub-database is connected with a signal through a controller, and data in any two sub-databases can be compared and analyzed.
In a preferred embodiment of the present invention, the status parameter information is classified and calculated, the parameter information with the same feature point is stored in the same sub-database, and is recorded as the same class of data, and different classes of data are stored in different sub-databases.
The second aspect of the invention provides a solution titration system for ralstonia solanacearum, comprising: the device comprises a memory and a processor, wherein the memory comprises a bacterial wilt solution measurement method program, and the bacterial wilt solution measurement method program is executed by the processor to realize the following steps:
acquiring a titration signal to obtain titration information, and establishing an initial titration rate curve according to the titration information;
carrying out solution titration according to titration information, detecting a solution absorption signal on the test strip at the same time, acquiring solution absorption information, and establishing a solution absorption rate curve;
acquiring corrected titration information according to the solution absorption information and the solution absorption rate curve;
and correcting the titration rate according to the corrected titration information.
In a preferred embodiment of the invention, solution titration is carried out according to titration information, and meanwhile, solution absorption signals on the test paper strip are detected, so that solution absorption information is obtained, and a solution absorption rate curve is established; the method comprises the following steps:
detecting the characteristics of the test strip, acquiring the absorption characteristics of the test strip, and establishing a test strip absorption information database;
screening database information, and removing bad points and deviation data of the data;
extracting effective information of a database, and analyzing and obtaining solution absorption information;
establishing an information model according to the solution absorption information;
and analyzing the information model to obtain a solution absorption rate curve.
In a preferred embodiment of the present invention, the titration rate is corrected according to the corrected titration information; the method specifically comprises the following steps:
obtaining initial titration information according to a titration mathematical model;
extracting solution absorption information through data index to obtain corresponding corrected titration information;
obtaining titration deviation information by correcting titration information to obtain titration deviation rate;
judging whether the titration deviation rate is larger than a preset deviation rate threshold value or not;
and if the deviation rate is larger than the preset deviation rate threshold value, performing reverse correction adjustment for correcting the titration information.
The invention solves the defects existing in the background technology, and has the following beneficial effects:
1. the test strip absorption characteristics are obtained by detecting the test strip characteristics, and a test strip absorption information database is established; the method can be used for screening database information, eliminating bad points and deviation data of the data, extracting effective information of the database and obtaining a solution absorption rate curve, and the method can be used for extracting the data with larger deviation actual values, so that the data obtained after analysis is closer to the actual values.
2. And the titration rate is subjected to synchronous error correction through the correction matrix, zero drift is removed, and the system response is faster.
3. According to the real-time monitoring solution titration information, early judgment can be carried out through correction processing information, the hysteresis of a system is reduced, discrete distribution nodes are established in the process of determining correction information, and drift nodes are removed by using a trend removal algorithm; extracting curve space state parameters and establishing a database; the solution absorption information is extracted through the data index, the corresponding corrected titration information is obtained, the analysis speed is high through the analysis mode, the gait information after aggregation simulation is closer to reality, and the corrected gait information effect is good.
Drawings
In order that the above-recited objects, features and advantages of the present invention will be more clearly understood, a more particular description of the invention will be rendered by reference to the appended drawings and appended detailed description. It should be noted that, in the case of no conflict, the embodiments of the present application and the features in the embodiments may be combined with each other.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, however, the present invention may be practiced in other ways than those described herein, and therefore the scope of the present invention is not limited to the specific embodiments disclosed below.
FIG. 1 is a block diagram showing a method for measuring bacterial wilt in the present invention.
FIG. 2 is a flow chart of a method for acquiring an initial titration rate curve of a bacterial wilt solution.
FIG. 3 is a flow chart of a method for acquiring the absorption rate curve of the solution of bacterial wilt in the invention.
FIG. 4 is a flow chart of a method for correcting titration information of a bacterial wilt solution.
FIG. 5 is a block diagram of a solution measurement system for bacterial wilt in accordance with the present invention.
Detailed Description
In order that the above-recited objects, features and advantages of the present invention will be more clearly understood, a more particular description of the invention will be rendered by reference to the appended drawings and appended detailed description. It should be noted that, in the case of no conflict, the embodiments of the present application and the features in the embodiments may be combined with each other.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, however, the present invention may be practiced in other ways than those described herein, and therefore the scope of the present invention is not limited to the specific embodiments disclosed below.
As shown in FIG. 1, the invention discloses a block diagram of a solution determination method of bacterial wilt bacteria.
S102, a first sensor is arranged on a test strip, a titration signal of a bacterial wilt solution is monitored through the first sensor, instantaneous titration information is obtained, and a titration rate curve is established according to the titration information;
s104, a plurality of test solution channels are arranged on the test strip at intervals, solution titration is carried out according to titration information, the titrated solution flows in the test solution channels, meanwhile, the flow information of the test solution in the test solution channels is detected, and the solution absorption information of the test strip is obtained;
s106, monitoring the temperature of the solution of the bacterial wilt bacteria through a second sensor, determining solution temperature control information, obtaining temperature control interference information, and obtaining correction information through a trend removal algorithm through the temperature control interference information;
s108, establishing a titration mathematical model according to Matlab to obtain initial titration information, establishing a database, extracting solution absorption information through data index to obtain corresponding corrected titration information;
s110, obtaining titration deviation information by correcting titration information to obtain titration deviation rate, and judging whether the titration deviation rate is larger than a preset deviation rate threshold value;
s112, if the deviation rate is larger than a preset deviation rate threshold value, performing reverse correction adjustment of the corrected titration information;
s114, determining a measurement mode according to the solution absorption information and the correction information, obtaining measurement information, and synchronously displaying the measurement information through a display.
As shown in FIG. 2, the invention discloses a flow chart of a method for acquiring an initial titration rate curve of a bacterial wilt solution.
According to the embodiment of the invention, a titration signal is acquired to obtain titration information, and an initial titration rate curve is established according to the titration information; the method specifically comprises the following steps:
s202, acquiring a titration signal, simultaneously acquiring the information of a test solution channel, and establishing a titration mathematical model;
s204, obtaining initial titration information according to a titration mathematical model;
s206, analyzing initial titration information to obtain initial titration rate;
s208, establishing a titration rate curve according to the initial titration rate.
As shown in FIG. 3, the invention discloses a flow chart of a method for acquiring a solution absorption rate curve of bacterial wilt.
According to the embodiment of the invention, solution titration is carried out according to titration information, meanwhile, a solution absorption signal on a test paper strip is detected, solution absorption information is obtained, and a solution absorption rate curve is established; the method comprises the following steps:
s302, detecting characteristics of a test strip, acquiring absorption characteristics of the test strip, and establishing a test strip absorption information database;
s304, screening database information, and eliminating bad points and deviation data of data;
s306, extracting effective information of a database, and analyzing and obtaining solution absorption information;
s308, establishing an information model according to the solution absorption information;
s310, analyzing the information model to obtain a solution absorption rate curve.
As shown in FIG. 4, the invention discloses a flow chart of a method for correcting titration information of a bacterial wilt solution.
According to the embodiment of the invention, the corrected titration information is obtained according to the solution absorption information and the solution absorption rate curve; the method specifically comprises the following steps:
s402, performing curve segmentation extraction according to the solution absorption rate curve to obtain extraction information;
s404, according to the extracted information, discrete distribution nodes are established, and drift nodes are removed by using a trend removal algorithm;
s406, extracting curve space state parameters and establishing a database;
and S408, extracting solution absorption information through the data index to obtain corresponding corrected titration information.
The database is divided into a plurality of sub-databases, each sub-database is connected with a signal through a controller, and data in any two sub-databases can be compared and analyzed.
And carrying out classifying calculation on the state parameter information, storing the parameter information with the same characteristic point into the same sub-database, marking the parameter information as the same type of data, and storing different types of data into different sub-databases.
As shown in FIG. 5, the invention discloses a block diagram of a solution measurement system of bacterial wilt bacteria.
In a second aspect, the present invention provides a solution titration system for ralstonia solanacearum, the system 5 comprising: the device comprises a memory 51 and a processor 52, wherein the memory 51 comprises a bacterial wilt solution measurement method program, and the bacterial wilt solution measurement method program realizes the following steps when being executed by the processor:
acquiring a titration signal to obtain titration information, and establishing an initial titration rate curve according to the titration information;
carrying out solution titration according to titration information, detecting a solution absorption signal on the test strip at the same time, acquiring solution absorption information, and establishing a solution absorption rate curve;
acquiring corrected titration information according to the solution absorption information and the solution absorption rate curve;
and correcting the titration rate according to the corrected titration information.
The first sensor is arranged on the test strip, a titration signal of the bacterial wilt solution is monitored through the first sensor, instant titration information is obtained, and a titration rate curve is established according to the titration information; the plurality of test solution channels are arranged on the test strip at intervals, solution titration is carried out according to titration information, the titrated solution flows in the test solution channels, meanwhile, the flow information of the test solution in the test solution channels is detected, and the absorption information of the test strip solution is obtained; monitoring the temperature of the solution of the bacterial wilt bacteria through a second sensor, determining solution temperature control information, obtaining temperature control interference information, and obtaining correction information through a trend removal algorithm through the temperature control interference information; establishing a titration mathematical model according to Matlab to obtain initial titration information, establishing a database, and extracting solution absorption information through data index to obtain corresponding corrected titration information; obtaining titration deviation information through correcting the titration information to obtain titration deviation rate, judging whether the titration deviation rate is larger than a preset deviation rate threshold value, and if so, carrying out reverse correction adjustment of the corrected titration information; and determining a measurement mode according to the solution absorption information and the correction information to obtain measurement information, and synchronously displaying the measurement information through a display.
According to the embodiment of the invention, solution titration is carried out according to titration information, meanwhile, a solution absorption signal on a test paper strip is detected, solution absorption information is obtained, and a solution absorption rate curve is established; the method comprises the following steps:
detecting the characteristics of the test strip, acquiring the absorption characteristics of the test strip, and establishing a test strip absorption information database;
screening database information, and removing bad points and deviation data of the data;
extracting effective information of a database, and analyzing and obtaining solution absorption information;
establishing an information model according to the solution absorption information;
and analyzing the information model to obtain a solution absorption rate curve.
According to the embodiment of the invention, the titration rate is corrected according to the corrected titration information; the method specifically comprises the following steps:
obtaining initial titration information according to a titration mathematical model;
extracting solution absorption information through data index to obtain corresponding corrected titration information;
obtaining titration deviation information by correcting titration information to obtain titration deviation rate;
judging whether the titration deviation rate is larger than a preset deviation rate threshold value or not;
and if the deviation rate is larger than the preset deviation rate threshold value, performing reverse correction adjustment for correcting the titration information.
According to the embodiment of the invention, a titration signal is acquired to obtain titration information, and an initial titration rate curve is established according to the titration information; the method specifically comprises the following steps:
acquiring a titration signal, acquiring the information of a test solution channel, and establishing a titration mathematical model;
obtaining initial titration information according to a titration mathematical model;
analyzing initial titration information to obtain initial titration rate;
a titration rate curve is established based on the initial titration rate.
According to the embodiment of the invention, solution titration is carried out according to titration information, meanwhile, a solution absorption signal on a test paper strip is detected, solution absorption information is obtained, and a solution absorption rate curve is established; the method comprises the following steps:
detecting the characteristics of the test strip, acquiring the absorption characteristics of the test strip, and establishing a test strip absorption information database;
screening database information, and removing bad points and deviation data of the data;
extracting effective information of a database, and analyzing and obtaining solution absorption information;
establishing an information model according to the solution absorption information;
and analyzing the information model to obtain a solution absorption rate curve.
According to the embodiment of the invention, the corrected titration information is obtained according to the solution absorption information and the solution absorption rate curve; the method specifically comprises the following steps:
performing curve segmentation extraction according to the solution absorption rate curve to obtain extraction information;
according to the extracted information, discrete distribution nodes are established, and drift nodes are removed by using a trending removal algorithm;
extracting curve space state parameters and establishing a database;
and extracting solution absorption information through data index to obtain corresponding corrected titration information.
In the several embodiments provided in this application, it should be understood that the disclosed apparatus and method may be implemented in other ways. The above described device embodiments are only illustrative, e.g. the division of the units is only one logical function division, and there may be other divisions in practice, such as: multiple units or components may be combined or may be integrated into another system, or some features may be omitted, or not performed. In addition, the various components shown or discussed may be coupled or directly coupled or communicatively coupled to each other via some interface, whether indirectly coupled or communicatively coupled to devices or units, whether electrically, mechanically, or otherwise.
The units described above as separate components may or may not be physically separate, and components shown as units may or may not be physical units; can be located in one place or distributed to a plurality of network units; some or all of the units may be selected according to actual needs to achieve the purpose of the solution of this embodiment.
In addition, each functional unit in each embodiment of the present invention may be integrated in one processing unit, or each unit may be separately used as one unit, or two or more units may be integrated in one unit; the integrated units may be implemented in hardware or in hardware plus software functional units.
Those of ordinary skill in the art will appreciate that: all or part of the steps for implementing the above method embodiments may be implemented by hardware related to program instructions, and the foregoing program may be stored in a computer readable storage medium, where the program, when executed, performs steps including the above method embodiments; and the aforementioned storage medium includes: a mobile storage device, a Read-Only Memory (ROM), a random access Memory (RAM, random Access Memory), a magnetic disk or an optical disk, or the like, which can store program codes.
Alternatively, the above-described integrated units of the present invention may be stored in a computer-readable storage medium if implemented in the form of software functional modules and sold or used as separate products. Based on such understanding, the technical solutions of the embodiments of the present invention may be embodied in essence or a part contributing to the prior art in the form of a software product stored in a storage medium, including several instructions for causing a computer device (which may be a personal computer, a server, or a network device, etc.) to execute all or part of the methods described in the embodiments of the present invention. And the aforementioned storage medium includes: a removable storage device, ROM, RAM, magnetic or optical disk, or other medium capable of storing program code.
The foregoing is merely illustrative of the present invention, and the present invention is not limited thereto, and any person skilled in the art will readily recognize that variations or substitutions are within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (5)
1. A method for determining a bacterial wilt solution comprises the following steps: a first sensor and a second sensor, characterized in that,
the first sensor is arranged on the test strip, monitors titration signals of the bacterial wilt bacteria solution through the first sensor to obtain instantaneous titration information, and establishes a titration rate curve according to the titration information;
the plurality of test solution channels are arranged on the test strip at intervals, solution titration is carried out according to titration information, the titrated solution flows in the test solution channels, meanwhile, the flow information of the test solution in the test solution channels is detected, and the absorption information of the test strip solution is obtained;
monitoring the temperature of the solution of the bacterial wilt bacteria through a second sensor, determining solution temperature control information, obtaining temperature control interference information, and obtaining correction information through a trend removal algorithm through the temperature control interference information;
establishing a titration mathematical model to obtain initial titration information, establishing a database, extracting solution absorption information through data index to obtain corresponding corrected titration information;
obtaining titration deviation information by correcting titration information to obtain titration deviation rate, and judging whether the titration deviation rate is larger than a preset deviation rate threshold value;
if the deviation rate is larger than the preset deviation rate threshold value, performing reverse correction and adjustment of the corrected titration information;
determining a measurement mode according to the solution absorption information and the correction information to obtain measurement information, and synchronously displaying the measurement information through a display;
acquiring a titration signal to obtain titration information, and establishing a titration rate curve according to the titration information; the method specifically comprises the following steps:
acquiring a titration signal, acquiring the information of a test solution channel, and establishing a titration mathematical model;
obtaining initial titration information according to a titration mathematical model;
analyzing initial titration information to obtain initial titration rate;
establishing a titration rate curve according to the initial titration rate;
solution titration is carried out according to titration information, meanwhile, flow information of test solution is detected, and solution absorption information of a test strip is obtained, specifically:
detecting the characteristics of the test strip, acquiring the absorption characteristics of the test strip, and establishing a test liquid flow database and a titration information database;
screening database information, and removing bad points and deviation data of the data;
extracting effective information of a database, and analyzing and obtaining flow information and titration information of a test solution;
according to the flow information of the test solution, an information model is established;
analyzing the information model to obtain the absorption information of the test strip solution;
acquiring corrected titration information according to the solution absorption information and the solution absorption rate curve; the method specifically comprises the following steps:
performing curve segmentation extraction according to the solution absorption rate curve to obtain extraction information;
according to the extracted information, discrete distribution nodes are established, and drift nodes are removed by using a trending removal algorithm;
extracting curve space state parameters and establishing a database;
and extracting solution absorption information through data index to obtain corresponding corrected titration information.
2. The method for determining bacterial wilt solution according to claim 1, wherein the database is divided into a plurality of sub-databases, each sub-database is connected with a signal through a controller, and data in any two sub-databases can be compared and analyzed.
3. The method for determining bacterial wilt solution according to claim 2, wherein the status parameter information is classified and calculated, the parameter information having the same characteristic point is stored in the same sub-database and recorded as the same kind of data, and the different kinds of data are stored in different sub-databases.
4. A solution titration system for ralstonia solanacearum, the system comprising: the device comprises a memory and a processor, wherein the memory comprises a bacterial wilt solution measurement method program, and the bacterial wilt solution measurement method program is executed by the processor to realize the following steps:
acquiring a titration signal to obtain titration information, and establishing a titration rate curve according to the titration information;
solution titration is carried out according to titration information, meanwhile, flow information of a test solution is detected, and absorption information of the test strip solution is obtained;
acquiring the temperature of the solution, determining the temperature control information of the solution to obtain the temperature control interference information,
acquiring correction information by using a trend removal algorithm through the temperature control interference information;
determining a measurement mode according to the solution absorption information and the correction information to obtain measurement information;
synchronously displaying the measurement information;
solution titration is carried out according to titration information, meanwhile, flow information of a test solution is detected, and absorption information of the test strip solution is obtained; the method comprises the following steps:
detecting the characteristics of the test strip, acquiring the absorption characteristics of the test strip, and establishing a test liquid flow database and a titration information database;
screening database information, and removing bad points and deviation data of the data;
extracting effective information of a database, and analyzing and obtaining flow information and titration information of a test solution;
according to the flow information of the test solution, an information model is established;
and analyzing the information model to obtain the absorption information of the test strip solution.
5. The solution titration system for bacterial wilt of claim 4, wherein the titration rate is modified based on the modified titration information; the method specifically comprises the following steps:
obtaining initial titration information according to a titration mathematical model;
extracting solution absorption information through data index to obtain corresponding corrected titration information;
obtaining titration deviation information by correcting titration information to obtain titration deviation rate;
judging whether the titration deviation rate is larger than a preset deviation rate threshold value or not;
and if the deviation rate is larger than the preset deviation rate threshold value, performing reverse correction adjustment for correcting the titration information.
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