CN111467561A

CN111467561A - Dressing containing trehalose and capable of repairing skin screen and manufacturing process of dressing

Info

Publication number: CN111467561A
Application number: CN202010435484.8A
Authority: CN
Inventors: 舒凌
Original assignee: Chayan Biotechnology Co Ltd
Current assignee: Chunyan Biotechnology Co ltd
Priority date: 2020-05-21
Filing date: 2020-05-21
Publication date: 2020-07-31
Anticipated expiration: 2040-05-21
Also published as: CN111467561B

Abstract

The invention provides a dressing containing trehalose for repairing a skin screen and a manufacturing process thereof, wherein the dressing comprises the following raw materials in parts by weight: 55-100 parts of trehalose, 13-25 parts of sea buckthorn polysaccharide, 65-95 parts of glycerol, 32-46 parts of sweet almond oil, 13-20 parts of helicase, 25-35 parts of pentanediol, 3.0-5.0 parts of phenoxyethanol, 2.3-3.5 parts of triethanolamine and 765-852 parts of water.

Description

Dressing containing trehalose and capable of repairing skin screen and manufacturing process of dressing

Technical Field

The invention relates to the field of biomedicine, in particular to a dressing containing trehalose and capable of repairing a skin screen barrier and a manufacturing process thereof.

Background

In recent years, the medical plastic beauty industry of China is different military prominence, the development is rapid, the demand market is vigorous, beauty in the 21 st century is a necessary living need of young women, laser treatment is a novel medical beauty technology which is made up at present, most of patients with skin problems need to receive laser treatment, laser beauty actually utilizes lasers with different types and wavelengths to cause certain influence on skin tissues, for example, the heat effect generated by the laser treatment can cause the reduction of the skin barrier function, so that the skin becomes sensitive, the main expression is inflammatory cell infiltration around dermal blood vessels, the epidermis has intracellular edema and intercellular edema, and erythema, swelling and even blister are generated; at present, products aiming at repairing after the face laser operation are diversified in the market, but the problems of poor repairing effect, long repairing time and the like exist.

Disclosure of Invention

In view of the above, the present invention provides a dressing containing trehalose capable of repairing a skin screen and a manufacturing process thereof, which solve the above problems.

The technical scheme of the invention is realized as follows: a dressing containing trehalose for repairing skin screen barrier: the feed comprises the following raw materials in parts by weight: 55-100 parts of trehalose, 13-25 parts of sea buckthorn polysaccharide, 65-95 parts of glycerol, 32-46 parts of sweet almond oil, 13-20 parts of helicase, 25-35 parts of pentanediol, 3.0-5.0 parts of phenoxyethanol, 2.3-3.5 parts of triethanolamine and 765-852 parts of water.

Further, the skin barrier trehalose dressing comprises the following raw materials in parts by weight: 73 parts of trehalose, 20 parts of sea buckthorn polysaccharide, 80 parts of glycerol, 39 parts of sweet almond oil, 18 parts of helicase, 30 parts of pentanediol, 4.0 parts of phenoxyethanol, 3.1 parts of triethanolamine and 805 parts of water.

Further, the trehalose comprises α -trehalose, α -trehalose and β -trehalose in a mass ratio of (2-5) to (5-30).

Further, the water is one of purified water, distilled water and softened water.

Further, a process for manufacturing a skin barrier trehalose dressing, comprising the steps of:

s1, adding water accounting for 80% of the total amount into a preparation tank, adding trehalose and sea buckthorn polysaccharide in a stirring state in a grading manner, stirring for dissolving, wherein the stirring frequency is 20-30 Hz, and the stirring time is 20-40 min, so as to obtain a phase A;

s2, adding 1.3-2.0 times of composite phosphate solution into the phase A, placing the phase A in a water bath oscillator, adjusting the pH value to 6-8, and oscillating for 50-80 min at the temperature of 50-70 ℃ to obtain a phase B;

s3, centrifuging the phase B at 5000-7000 rpm for 20-30 min, ultra-filtering the supernatant obtained by centrifugation with an ultra-filtration membrane, allowing the ultra-filtered permeate to pass through an acid-base column at a flow rate of 3-5 m L/S, collecting the liquid passing through the acid-base column as phase C,

and S4, sequentially adding glycerol, sweet almond oil, helicase, pentanediol and phenoxyethanol into the phase C, adding the rest water, stirring uniformly, stirring at the frequency of 20-30 Hz for 25-35 min, and regulating the pH value of the solution to 4.5-6.5 by using triethanolamine to obtain the skin barrier trehalose dressing.

Further, the composite phosphate is prepared by uniformly mixing sodium tripolyphosphate and sodium trimetaphosphate according to the mass ratio of (1.3-2.0) to (0.4-1.0).

Compared with the prior art, the invention has the beneficial effects that:

(1) the raw materials are selected according to the skin repair mechanism, the raw materials are scientifically proportioned and cooperatively play a role, the skin repair effect is improved, and skin allergy caused after laser treatment is avoided, wherein α -trehalose, α -trehalose, β -trehalose and hippophae rhamnoides polysaccharide are mixed according to a proportion, can effectively enter cells to play a unique role of water substitution stress factors, can form a layer of protective film on the cell surface layer, α -trehalose and β -trehalose can fully lock skin moisture, reduce evaporation of facial moisture of the skin, and enable the moisture to be freely diffused to the horny layer, so that the cell membrane is effectively protected;

(2) secondly, the glycerol, the sweet almond oil and the helicase are added into the phosphorylated polysaccharide solution, phosphate radical ions can be promoted to be fully combined with trehalose hydroxyl and sea buckthorn polysaccharide glycosidic bonds, the anti-freezing activity of sugar molecules is improved, the molecular structure of cell membrane components is stabilized, damage caused by changes of the membrane is reduced, when the combination of the helicase, the sweet almond oil, the pentanediol and the phenoxyethanol permeates into skin surface cells of a human body, the viscosity of the whole cells and the concentration of solutes in the cells are increased, and therefore the phenomenon that the skin is inflamed due to uneven concentrations of the solutes and the solution in the cells is avoided.

(3) In the preparation process, the stirring frequency and time are strictly controlled, and the centrifugal liquid is centrifuged at a certain speed to obtain a purified centrifugate, wherein the composite phosphate solution is used for oscillating to obtain a phase B, so that phosphate ions can be fully combined with trehalose hydroxyl and a seabuckthorn polysaccharide glycosidic bond, the frost resistance activity of sugar molecules is improved, and the skin allergy symptom is relieved.

Detailed Description

In order to better understand the technical content of the invention, specific examples are provided below to further illustrate the invention.

The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified.

The materials, reagents and the like used in the examples of the present invention can be obtained commercially without specific description.

Example 1

A dressing containing trehalose for repairing skin screen barrier comprises the following raw materials, by weight, 55 parts of trehalose, 13 parts of sea buckthorn polysaccharide, 65 parts of glycerol, 32 parts of sweet almond oil, 13 parts of helicase, 25 parts of pentanediol, 3.0 parts of phenoxyethanol, 2.3 parts of triethanolamine and 765 parts of water, wherein α -trehalose, α -trehalose and β -trehalose are contained in the trehalose components in a mass ratio of 1:2: 5.

Example 2

A dressing containing trehalose for repairing skin screen barrier comprises the following raw materials, by weight, 100 parts of trehalose, 25 parts of sea buckthorn polysaccharide, 95 parts of glycerol, 46 parts of sweet almond oil, 20 parts of helicase, 35 parts of pentanediol, 5.0 parts of phenoxyethanol, 3.5 parts of triethanolamine and 852 parts of water, wherein α -trehalose, α -trehalose and β -trehalose are contained in the trehalose, and the trehalose is in a mass ratio of 1:5: 30.

Example 3

A dressing containing trehalose for repairing skin screen barrier comprises the following raw materials, by weight, 73 parts of trehalose, 20 parts of sea buckthorn polysaccharide, 80 parts of glycerol, 39 parts of sweet almond oil, 18 parts of helicase, 30 parts of pentanediol, 4.0 parts of phenoxyethanol, 3.1 parts of triethanolamine and 805 parts of water, wherein α -trehalose, α -trehalose and β -trehalose are contained in the trehalose, and the trehalose is in a mass ratio of 1:3: 18.

The skin barrier trehalose dressings of examples 1-3 above were prepared using the following method:

s1, adding water accounting for 80% of the total amount into a preparation tank, adding trehalose and sea buckthorn polysaccharide in a stirring state, stirring for dissolving, wherein the stirring frequency is 25Hz, and the stirring time is 30min to obtain a phase A;

s2, adding the phase A into 1.7 times of the composite phosphate solution, placing the mixture in a water bath oscillator, adjusting the pH value to 7, and oscillating the mixture for 60min at the temperature of 60 ℃ to obtain a phase B; the composite phosphate is prepared by uniformly mixing sodium tripolyphosphate and sodium trimetaphosphate in a mass ratio of 1.8: 0.7;

s3, centrifuging the phase B at 6000rpm for 25min, ultrafiltering the supernatant with ultrafiltration membrane, passing the ultrafiltration permeate through acid-base column at flow rate of 4m L/S, collecting the C phase,

and S4, sequentially adding glycerol, sweet almond oil, helicase, pentanediol and phenoxyethanol into the phase C, adding the rest water, stirring uniformly, stirring at the frequency of 25Hz for 30min, and regulating the pH value of the solution to 5.5 by using triethanolamine to obtain the skin barrier trehalose dressing.

Example 4

A dressing containing trehalose for repairing skin screen barrier comprises the following raw materials, by weight, 73 parts of trehalose, 20 parts of sea buckthorn polysaccharide, 80 parts of glycerol, 39 parts of sweet almond oil, 18 parts of helicase, 30 parts of pentanediol, 4.0 parts of phenoxyethanol, 3.1 parts of triethanolamine and 805 parts of water, wherein α -trehalose, α -trehalose and β -trehalose are contained in the trehalose, and the trehalose is contained in the trehalose, wherein the trehalose is contained in the trehalose, and the trehalose is;

the skin barrier trehalose dressing was prepared using the following method:

s1, adding water accounting for 80% of the total amount into a preparation tank, adding trehalose and sea buckthorn polysaccharide in a stirring state, stirring for dissolving, wherein the stirring frequency is 20Hz, and the stirring time is 20min to obtain a phase A;

s2, adding the phase A into 1.3 times of the composite phosphate solution, placing the mixture in a water bath oscillator, adjusting the pH value to 6, and oscillating the mixture for 50min at the temperature of 50 ℃ to obtain a phase B; the composite phosphate is prepared by uniformly mixing sodium tripolyphosphate and sodium trimetaphosphate in a mass ratio of 1.3: 0.4;

s3, centrifuging the phase B at 5000rpm for 20min, ultrafiltering the supernatant with ultrafiltration membrane, passing the ultrafiltration permeate through acid-base column at flow rate of 3m L/S, collecting the C phase,

and S4, sequentially adding glycerol, sweet almond oil, helicase, pentanediol and phenoxyethanol into the phase C, adding the rest water, stirring uniformly, stirring at the frequency of 20Hz for 25min, and regulating the pH value of the solution to 4.5 by using triethanolamine to obtain the skin barrier trehalose dressing.

Example 5

the skin barrier trehalose dressing was prepared using the following method:

s1, adding water accounting for 80% of the total amount into a preparation tank, adding trehalose and sea buckthorn polysaccharide in a stirring state, stirring for dissolving, wherein the stirring frequency is 30Hz, and the stirring time is 40min to obtain a phase A;

s2, adding 2.0 times of composite phosphate solution into the phase A, placing the phase A in a water bath oscillator, adjusting the pH value to 8, and oscillating for 80min at the temperature of 70 ℃ to obtain a phase B; the composite phosphate is prepared by uniformly mixing sodium tripolyphosphate and sodium trimetaphosphate according to the mass ratio of 2.0: 1.0;

s3, centrifuging the phase B at 7000rpm for 30min, ultrafiltering the supernatant with ultrafiltration membrane, passing the ultrafiltration permeate through acid-base column at flow rate of 5m L/S, collecting the C phase,

and S4, sequentially adding glycerol, sweet almond oil, helicase, pentanediol and phenoxyethanol into the phase C, adding the rest water, stirring uniformly, stirring at the frequency of 30Hz for 35min, and regulating the pH value of the solution to 6.5 by using triethanolamine to obtain the skin barrier trehalose dressing.

Example 6

This example differs from example 3 in that the trehalose component is α -trehalose.

Example 7

This example differs from example 3 in that: phase A was not added to the composite phosphate solution and shaken in a water bath.

Example 8

This example differs from example 3 in that: in step S3, phase B is centrifuged at 8000rpm for 25 min.

Example 9

This example differs from example 3 in that: the composite phosphate is sodium tripolyphosphate and sodium trimetaphosphate in a mass ratio of (1.3-2.0) to (0.4-1.0).

Comparative example 1

This comparative example differs from example 3 in that: a dressing containing trehalose for repairing skin screen comprises the following raw materials in parts by weight: 50 parts of trehalose, 12 parts of sea buckthorn polysaccharide, 55 parts of glycerol, 50 parts of sweet almond oil, 10 parts of helicase, 40 parts of pentanediol, 6 parts of phenoxyethanol, 2 parts of triethanolamine and 750 parts of water.

Comparative example 2

This comparative example differs from example 3 in that: a dressing containing trehalose for repairing skin screen comprises the following raw materials in parts by weight: 50 parts of trehalose, 12 parts of sea buckthorn polysaccharide, 80 parts of glycerol, 39 parts of sweet almond oil, 18 parts of helicase, 30 parts of pentanediol, 4.0 parts of phenoxyethanol, 3.1 parts of triethanolamine and 805 parts of water.

Comparative example 3

This comparative example differs from example 3 in that: a dressing containing trehalose for repairing skin screen comprises the following raw materials in parts by weight: 73 parts of trehalose, 20 parts of sea buckthorn polysaccharide, 55 parts of glycerol, 50 parts of sweet almond oil, 10 parts of helicase, 40 parts of pentanediol, 6 parts of phenoxyethanol, 2 parts of triethanolamine and 750 parts of water.

Comparative example 4

This comparative example differs from example 3 in that: a dressing containing trehalose for repairing skin screen is free of fructus Hippophae polysaccharide.

Comparative example 5

This comparative example differs from example 3 in that: a dressing containing trehalose for repairing skin screen barrier contains helicase replaced by protease.

Comparative example 6

This comparative example differs from example 3 in that: a dressing containing trehalose for repairing skin screen barrier is prepared by replacing semen Caryae Cathayensis oil with oleum Lini.

First, animal skin sensitization test

(1) The standard of the test basis is GB/T16886.10-2017 medical instrument biological evaluation

(2) Test system:

animal species: guinea pig

Animal number: 75 are provided with

Body weight range: 316.9g-499.7g

(3) Animal selection: healthy, early adult, previously unused test animals were selected, and females were infertile and infertile.

(4) The test sample, normal saline and 0.2g/m L2-mercaptobenzothiazole sodium carbonate solution are acted on each test group animal according to the method required by the standard, 3 days of repeated induction are continued in one week, 3 weeks are operated according to the method, 13 days after the last induction, the excitation test is carried out on the spine side skin which is not subjected to test treatment on the test animal, the erythema and edema conditions of the skin of each group animal are observed 24 hours and 48 hours after the dressing is removed, and the erythema and the edema are graded.

(5) And (3) animal observation: the skin condition at the challenge sites was observed in both the test and control animals 24h and 48h after dressing removal, and the skin response was described for each challenge site and each observation time using the classification score system specified in table 1 below.

(6)75 guinea pigs were randomly divided into 15 groups, and the dressings prepared in examples 1 to 9 of the present invention and comparative examples 1 to 6 were applied to the guinea pigs and tested, and the test results were as follows:

as can be seen from the above table, the raw materials used in the invention have no sensitization reaction according to reasonable proportion.

Second, the red swelling subsides

(1) 160 volunteers aged 16-40 years, half of both men and women, and the facial skin were treated by laser treatment, and divided into 16 groups of 10 persons each, using the dressings of examples 1-9 and comparative examples 1-6 and the commercial trehalose repair dressing, respectively, during which no repair product was used.

(2) The using method comprises the following steps: after cleaning the face, the product was applied to the repaired site, gently massaged until absorption, once in the morning and evening, and after 7 days of use, the therapeutic effect was recorded.

(3) And (3) effect measurement:

the effect is shown: the red swelling subsides by more than 40 percent, and the symptoms are relieved

The method has the following advantages: the red swelling is reduced by 15-40%, and the symptom is improved

And (4) invalidation: the red swelling subsided < 15% and the clinical symptoms were improved.

(4) The specific therapeutic effects are as follows:

the above table shows that the effect of the skin barrier trehalose dressing is better than that of a trehalose repair dressing sold in the market, compared with examples 1-9 and comparative examples 1-3, the raw materials used in the skin barrier trehalose dressing are scientifically proportioned, the obtained dressing can effectively improve skin redness and swelling, compared with examples 1-9 and comparative example 4, the repairing capability of the dressing can be improved by compounding the hippophae rhamnoides polysaccharide and the trehalose; compared with the comparative examples 5 and 6, the snailase can be combined with trehalose to protect cell membranes, and the sweet almond oil is combined with glycerin to promote skin comfort and anti-allergic capacity; compared with the examples 1 to 5 and 6, trehalose is compounded according to the proportion of the embodiment of the invention to achieve better effect, and compared with the examples 1 to 5 and 7, the phase A is reacted with a composite phosphate solution in the preparation process, so that phosphate ions can be fully combined with trehalose hydroxyl and a sea buckthorn polysaccharide glycosidic bond, and the frost resistance activity of sugar molecules is improved; comparing examples 1-5 with example 8, the purification effect of the solution is influenced by the centrifugal rate in the centrifugal process; examples 1-3 are compared with examples 4 and 5, which show that the selection of each process parameter has a certain influence on the effect of the dressing in the preparation process, and compared with examples 1-2 and 3, the scientific proportion of the raw materials in example 3 synergistically plays a role in the optimal proportion, so that the efficacy of the dressing in repairing damaged skin is improved.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims

1. A dressing that contains trehalose can restore skin screen barrier, its characterized in that: the feed comprises the following raw materials in parts by weight: 55-100 parts of trehalose, 13-25 parts of sea buckthorn polysaccharide, 65-95 parts of glycerol, 32-46 parts of sweet almond oil, 13-20 parts of helicase, 25-35 parts of pentanediol, 3.0-5.0 parts of phenoxyethanol, 2.3-3.5 parts of triethanolamine and 765-852 parts of water.

2. The dressing of claim 1, comprising a trehalose repairable skin screen, wherein: the feed comprises the following raw materials in parts by weight: 73 parts of trehalose, 20 parts of sea buckthorn polysaccharide, 80 parts of glycerol, 39 parts of sweet almond oil, 18 parts of helicase, 30 parts of pentanediol, 4.0 parts of phenoxyethanol, 3.1 parts of triethanolamine and 805 parts of water.

3. The dressing containing trehalose capable of repairing skin screen according to claim 1, wherein the trehalose comprises α -trehalose, α -trehalose and β -trehalose in a mass ratio of 1 (2-5) to (5-30).

4. The dressing of claim 1, comprising a trehalose repairable skin screen, wherein: the water is one of purified water, distilled water and softened water.

5. A process for manufacturing a dressing comprising a trehalose repairable skin screen according to claim 1, wherein: the method comprises the following steps:

6. A process for manufacturing a dressing comprising a trehalose repairable skin screen according to claim 5, wherein: the composite phosphate is prepared by uniformly mixing sodium tripolyphosphate and sodium trimetaphosphate according to the mass ratio of (1.3-2.0) to (0.4-1.0).