CN111458327A - Device for rapidly evaluating air bacterial pollution state - Google Patents
Device for rapidly evaluating air bacterial pollution state Download PDFInfo
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- CN111458327A CN111458327A CN202010433115.5A CN202010433115A CN111458327A CN 111458327 A CN111458327 A CN 111458327A CN 202010433115 A CN202010433115 A CN 202010433115A CN 111458327 A CN111458327 A CN 111458327A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
- G01N21/763—Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
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Abstract
The invention discloses a device for rapidly evaluating the air bacterial pollution state, which comprises a target particle separation and concentration system, a sampling system and a control system, wherein the target particle separation and concentration system is used for separating target particles in a sampling airflow and carrying out concentration and liquefaction treatment; the sample conveying and cracking system comprises a quantitative liquid transfer device connected with the target particle separation and concentration system, and an ultrasonic cracking reaction tank connected with the quantitative liquid transfer device through a pipeline, wherein the liquefied sample is subjected to cracking and luminous reaction in the ultrasonic cracking reaction tank; the bioluminescence detection system comprises a light receiving component for collecting the light intensity of the ultrasonic lysis reaction tank and a signal processing device connected with the light receiving component. The whole device integrates sampling and bioluminescence detection, and can quickly evaluate the air bacterial pollution state. The invention can be widely applied to the rapid analysis of the bacterial components of the bioaerosol, and has application prospects in the fields of atmospheric pollution research and monitoring, air microbial pollution analysis in indoor places, research and monitoring of airborne diseases and the like.
Description
Technical Field
The invention relates to a pollution evaluation device, in particular to a device for rapidly evaluating the air bacterial pollution state.
Background
In the research of indoor air microbial pollution, according to different research purposes, the microbial aerosol particles within a certain particle size range are often required to be separated and collected into specific liquid, and then offline analysis is performed. The traditional classical method for detection and analysis of airborne microorganisms is: (1) collecting air microorganisms on a medium, namely solid nutrient agar, semi-solid nutrient agar, a liquid medium and the like by using an air microorganism sampler; (2) there are many quantitative analysis methods, mainly culture analysis; (3) the qualitative analysis mainly includes biochemical analysis, nucleic acid detection analysis, sequencing analysis, etc. The quantitative analysis and the qualitative analysis of the air microorganisms are time-consuming and labor-consuming, and the use of consumables is more.
At present, whether to the microorganism in the atmosphere, still to the microorganism detection in the indoor air, mainly analyze concentration through the laboratory culture of sampling and sample, generally need 48 ~ 72hr, both hard, have time-consuming, can't realize the needs of real-time detection and on-line monitoring. The ATP bioluminescence technology is to take ATP in living microbe cells as a bioluminescence test target, measure the luminous intensity through a bioluminescence reaction and further estimate the concentration of living bacteria in a sample. The ATP bioluminescence detection technology can complete the detection of the concentration of the living microorganisms in the sample within the time of less than or equal to 5 min; the technology has been applied and researched for more than 30 years abroad, and the ATP bioluminescence technology has become a common method for evaluating the surface bacterial pollution. However, the air bacterial pollution detection evaluation is still very rare, and especially, no technology and device for integrating sampling and detection are available at present.
Disclosure of Invention
In view of the above problems, the present invention aims to provide a device for rapidly evaluating the air bacterial pollution state by integrating sampling and bioluminescence detection.
In order to achieve the purpose, the invention adopts the following technical scheme: an apparatus for rapidly evaluating air bacterial pollution state, comprising:
the target particle separation and concentration system is used for separating target particles in the sampling airflow and carrying out concentration and liquefaction treatment;
the sample conveying and cracking system comprises a quantitative liquid transfer device connected with the target ion particle separation and concentration system and an ultrasonic cracking reaction tank connected with the quantitative liquid transfer device through a pipeline, wherein the quantitative liquid transfer device is used for conveying a liquefied sample into the ultrasonic cracking reaction tank, and the liquefied sample is subjected to cracking and luminous reaction in the ultrasonic cracking reaction tank;
the bioluminescence detection system comprises a light receiving component for collecting the light intensity of the ultrasonic lysis reaction tank and a signal processing device connected with the light receiving component, wherein the signal processing device is used for converting a light intensity signal transmitted by the light receiving component into a target particle concentration.
Preferably, the target particle separating and concentrating system includes:
the target particle separation concentrator is used for carrying out separation and concentration treatment on the sampling airflow;
a sample collector, wherein a liquid medium is placed in the sample collector, the sample collector is connected with the target particle separation concentrator through a first pipeline, one end of the first pipeline is communicated with the target particle separation concentrator, the other end of the first pipeline extends into the liquid medium of the sample collector, the sample collector is connected with the quantitative liquid transfer device through a second pipeline, one end of the second pipeline extends into the liquid medium of the sample collector, and the other end of the second pipeline is communicated with the quantitative liquid transfer device;
and the exhaust fan is respectively connected with the target particle separation concentrator and the sample collector through a third pipeline.
Preferably, the target particle separation concentrator comprises a top cover, an air inlet cover, a first-stage flow guide plate, a first-stage impact plate, a second-stage flow guide plate, a sampling cavity, a second-stage collection plate, a second-stage exhaust hole plate, an air outlet, a bottom plate and a sample collection port;
a sampling port is reserved at the top of the air inlet cover, the top cover is connected to the sampling port of the air inlet cover, the first-stage guide plate and the second-stage impact plate are arranged in the air inlet cover at intervals up and down, the bottom of the air inlet cover is communicated with the top of the sampling cavity, a plurality of first guide holes are formed in the first-stage guide plate, a recess is formed in the top surface of the second-stage impact plate which is positioned right below the first guide holes, and a plurality of through holes are formed in the second-stage impact plate which is positioned outside the recess;
the second guide plate and the second exhaust hole plate are arranged in the sampling cavity at intervals up and down, and a plurality of second guide holes are formed in the second guide plate; the second-stage collection cavity is arranged between the second-stage guide plate and the second-stage exhaust hole plate, an annular gap is reserved between the second-stage collection cavity and the sampling cavity, the second-stage collection cavity comprises a collection cavity section, a conical cavity section and a straight cylinder cavity section which are sequentially communicated from top to bottom, the straight cylinder cavity section is fixed on the second-stage exhaust hole plate, and the lower part of the straight cylinder cavity section penetrates through the second-stage exhaust hole plate; the second-stage collecting plate is arranged in the collecting cavity section, a plurality of collecting holes are formed in the second-stage collecting plate, and the collecting holes are in one-to-one correspondence with the second diversion holes vertically;
the exhaust port is vertically arranged on the side wall of the sampling cavity, which is positioned on the second-stage exhaust hole plate in a penetrating manner; the bottom plate is fixedly arranged at the bottom of the sampling cavity; the sample collecting port is fixedly arranged on the bottom plate, and the upper part of the sample collecting port extends into the sampling cavity and corresponds to the lower part of the straight cylinder cavity section; the air outlet is connected with the exhaust fan through a third pipeline, and the lower part of the sample collecting port is connected with the sample collector through a first pipeline.
Preferably, a plurality of second baffle holes on the second baffle are positioned right below the recess on the first-stage impact plate;
the aperture of the through hole on the first-stage impact plate is larger than that of the first flow guide hole on the first-stage flow guide plate, and the aperture of the second flow guide hole is smaller than that of the first flow guide hole.
Preferably, the ultrasonic cracking reaction tank comprises an ultrasonic cracking groove, a cracking reaction tank inserted in the ultrasonic cracking groove, and a light transmission hole formed in the side wall of the ultrasonic cracking groove, and a cracking agent is placed in the cracking reaction tank; the light receiving assembly collects the light intensity of the lysis reaction tank through the light hole.
Preferably, the ultrasonic cracking tank is a tank body made of metal or plastic and has the volume of 20ml～30ml。
Preferably, the light receiving assembly comprises a photomultiplier tube for collecting light intensity of the ultrasonic lysis reaction cell, and the photomultiplier tube is connected with the signal processing device.
Preferably, the signal processing device is provided with a serial port and a USB interface which are connected with a computer and used for exporting data of sampling detection;
the device also comprises an electric control system for controlling the operation of the target particle separation and concentration system, the sample conveying and cracking system and the bioluminescence detection system.
Preferably, the target particle separation concentrator, the sample collector and the exhaust fan are integrally arranged in a first cabinet body, and a first cabinet door is reserved on the first cabinet body;
a power pump is arranged on a third pipeline between the exhaust fan and the target particle separation concentrator;
the sample collector adopts a collecting bottle, and the liquid medium in the collecting bottle is 20 ml.
Preferably, the quantitative pipettor and the ultrasonic lysis reaction tank are integrally arranged in the second cabinet body, and a second cabinet door is reserved on the second cabinet body.
By adopting the technical scheme, the invention has the following advantages:
1. the invention separates the target in the sampling air flow through the target particle separating and concentrating system, and carries out concentration and liquefaction treatment, cracking and luminescence reaction occur in the sample conveying and cracking system, the light receiving component in the bioluminescence detection system collects light intensity and transmits a light intensity signal to the signal processing device, the signal processing device converts the light intensity signal into the concentration of the target particle, namely the concentration of bacteria in the collected sample, the whole device integrates sampling and bioluminescence detection, can detect the sample in situ, and quickly evaluate the air bacterial pollution state. The invention can be widely applied to the rapid analysis of the bacterial components of the bioaerosol, and has application prospects in the fields of atmospheric pollution research and monitoring, air microbial pollution analysis in indoor places, research and monitoring of airborne diseases and the like.
2. The target particle separating and concentrating system comprises a target particle separating and concentrating device, a sample collector and an exhaust fan, wherein the exhaust fan mainly provides airflow flowing power, the target particle separating and concentrating device adopts a virtual impact principle, can remove large particles and small particles in a sample, retains particles with the particle size (D) range of 0.5 mu m-10 mu m, and can concentrate the target particles to be collected into airflow of 5L/min-15L/min, the target particle separating and concentrating device can also adopt other samplers capable of collecting airflow for separation and concentration, the sampling flow rate of the samplers is not limited, and the concentrated airflow containing the target particles impacts liquid media (media comprise sterile water, PBS buffer solution, 0.9% physiological saline and the like) in the sample collector to realize the liquefaction treatment of the sample.
3. The collected sample flows in a totally closed path formed by the target particle separation concentrator, the sample collector, the quantitative liquid transfer system and the cracking luminescence reaction system, so that possible manual operation pollution can be avoided, and the evaluation accuracy is improved.
4. The sample collector, the reaction tank, the pipeline, the lysate, the enzyme and the like are disposable consumables, and are replaced in different detection and evaluation processes, so that cross contamination is avoided.
5. The whole device of the invention controls the operation of the device through the electric control system, realizes the intelligent control of sampling, reduces the interference of human factors and obtains more objective results.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic diagram of the structure of the target particle separation concentrator of the present invention;
in the figure, 1, a target particle separation and concentration system; 10. a cabinet body; 11. a target particle separation concentrator; 12. a sample collector; 13. an exhaust fan; 111, a top cover, 112, an air inlet cover, 113, a first-stage guide plate, 114, a first-stage impact plate, 115 and a second-stage guide plate; 116. a sampling cavity 117, a second-stage collection cavity 118, a second-stage collection plate 119, a second-stage exhaust hole plate 1101, an exhaust hole 1102, a bottom plate 1103 and a sample collection port;
2. a sample transport lysis system; 20. a cabinet body; 21. a quantitative pipettor; 22. an ultrasonic pyrolysis reactor; 221, an ultrasonic cracking tank; 222. a pyrolysis reaction tank; 223. a light-transmitting hole;
3. a bioluminescent detection system; 31. a light receiving member; 311. a photomultiplier tube; 32. a signal processing device.
Detailed Description
The invention is described in detail below with reference to the figures and examples. It is to be understood, however, that the drawings are provided solely for the purposes of promoting an understanding of the invention and that they are not to be construed as limiting the invention.
As shown in fig. 1, the present invention provides an apparatus for rapidly evaluating the air bacterial contamination state, the apparatus comprising:
the target particle separation and concentration system 1 is used for separating target particles in a sampling airflow and carrying out concentration and liquefaction treatment;
the sample conveying and cracking system 2 comprises a quantitative liquid transfer device 21 connected with the target particle separating and concentrating system 1 and an ultrasonic cracking reaction tank device 22 connected with the quantitative liquid transfer device 21 through a pipeline, wherein the quantitative liquid transfer device 21 is used for conveying the liquefied sample into the ultrasonic cracking reaction tank device 22, and the liquefied sample is cracked and subjected to luminous reaction in the ultrasonic cracking reaction tank device 22;
the bioluminescence detection system 3 comprises a light receiving component 31 for collecting the light intensity of the ultrasonic lysis reaction cell 22, and a signal processing device 32 connected with the light receiving component 31, wherein the signal processing device 32 is used for converting the light intensity signal transmitted from the light receiving component 31 into the target particle concentration.
When the invention is used: the target particle separation and concentration system 1 separates target particles in a sampling airflow (aerosol particles in the atmosphere), concentrates and liquefies particles with the particle size of 0.5-10 microns, converts the particles into a liquid sample, a quantitative liquid transfer device 21 conveys the liquefied sample to an ultrasonic cracking reaction tank device 22, and the target particles in a hydraulic sample are cracked and subjected to a luminous reaction in the ultrasonic cracking reaction tank device 22; the light receiving component 31 collects light intensity, and the signal processing device 32 processes the light intensity signal transmitted from the light receiving component 31 and converts the light intensity signal into the concentration of target particles, namely the concentration of bacteria in the collected sample.
In one embodiment, the target particle separating and concentrating system 1 includes: a target particle separation concentrator 11 for performing separation and concentration processing on the sampling gas flow; a sample collector 12, in which a liquid medium is placed, wherein the sample collector 12 is connected with the target particle separation concentrator 11 through a first pipeline, one end of the first pipeline is communicated with the target particle separation concentrator 11, the other end of the first pipeline extends into the liquid medium of the sample collector 12, the sample collector 12 is connected with a quantitative pipette 21 through a second pipeline, one end of the second pipeline extends into the liquid medium of the sample collector 12, and the other end of the second pipeline is communicated with the quantitative pipette 21; and an exhaust fan 13 connected to the target particle separation and concentration unit 11 and the sample collector 12 through a third line, respectively. When the device is used, the exhaust fan 13 is started, the sampling airflow is sucked into the target particle separation concentrator 11, non-target particles separated from the target particle separation concentrator 11 enter the third pipeline along with the airflow and are discharged through the exhaust fan 13, the concentrated target particles enter the sample collector 12, a liquid medium is placed in the sample collector 12, and target particle gas is dissolved in the liquid medium to complete liquefaction treatment of the target particles to form a liquid sample; since the exhaust fan 13 sucks the gas in the sample collector 12 in advance, the target particle gas is prevented from being mixed with other gases.
In one embodiment, as shown in fig. 2, target particle separation concentrator 11 comprises top cap 111, air inlet cap 112, first stage flow guide plate 113, first stage impaction plate 114, second stage flow guide plate 115, sampling cavity 116, second stage collection chamber 117, second stage collection plate 118, second stage vent plate 119, vent 1101, bottom plate 1102, and sample collection port 1103;
a sampling port is reserved at the top of the air inlet cover 112, the top cover 111 is connected to the sampling port of the air inlet cover 112, the first-stage guide plate 113 and the second-stage impact plate 114 are arranged in the air inlet cover 112 at intervals up and down, and the bottom of the air inlet cover 112 is communicated with the top of the sampling cavity 116; a plurality of first flow guide holes are formed in the first-stage flow guide plate 113, a recess is formed in the top surface of the second-stage impact plate 114 located right below the first flow guide holes, and a plurality of through holes are formed in the second-stage impact plate 114 located outside the recess;
the second-stage guide plate 115 and the second-stage vent hole plate 119 are arranged in the sampling cavity 116 at intervals up and down, a plurality of second guide holes are formed in the second guide plate 115, the second-stage collection cavity 117 is arranged between the second-stage guide plate 115 and the second-stage vent hole plate 119, an annular gap is reserved between the second-stage collection cavity 117 and the sampling cavity 116, the second-stage collection cavity 117 comprises a collection cavity section 1171, a conical cavity section 1172 and a straight cylinder cavity section 1173 which are sequentially communicated from top to bottom, the straight cylinder cavity section 1173 is fixed on the second-stage vent hole plate 119, and the lower part of the straight cylinder cavity section 1173 penetrates through the second-stage vent hole plate 119; the second-stage collecting plate 118 is arranged in the collecting cavity section 1171, a plurality of collecting holes are formed in the second-stage collecting plate 118, and the collecting holes are in one-to-one correspondence with the second diversion holes from top to bottom;
the exhaust port 1101 is vertically arranged on the side wall of the sampling cavity 116 below the second-stage exhaust hole plate 119 in a penetrating manner; the bottom plate 1102 is fixedly disposed at the bottom of the sampling cavity 116; the sample collection port 1103 is fixedly arranged on the bottom plate 1102, and the upper part of the sample collection port 1103 extends into the sampling cavity 116 and corresponds to the lower part of the straight cylinder cavity section 1173; the exhaust port 1101 is connected to the suction fan 13 through a third pipe, and the lower portion of the sample collection port 103 is connected to the sample collector 12 through a first pipe.
When the target particle separation concentrator 11 in the embodiment is used, the top cover 111 is opened, particles enter the air inlet cover 112 along with total sampling airflow from a sampling port, after passing through a plurality of first flow guide holes on the first-stage flow guide plate 113, large particles directly impact the depressions on the first-stage impact plate 114, so that most of the particles larger than 10 microns are blocked, small particles pass through holes on the first-stage impact plate 114 along with the airflow, after passing through second flow guide holes on the second-stage flow guide plate 115, the particles larger than 0.5 microns directly pass through the collecting holes positioned right below the second flow guide holes under the action of inertia to enter the second-stage collecting cavity 117, so that most of the particles in the second-stage collecting cavity 117 are particles with the particle size of 0.5-10 microns, the particles with the particle size of less than 0.5 microns continuously move downwards through an annular gap between the second-stage collecting cavity 117 and the sampling cavity 116 under the action of the airflow, the particles pass through small holes of the second-stage air outlet plate 119 to enter a space between the second-stage exhaust hole plate 119 and the bottom plate 1102, and pass through the exhaust hole of the sampling cavity 117 along with the airflow, the sampling tube, the sampling.
In one embodiment, the target particle separating and concentrating device 11 may also adopt other samplers capable of collecting the airflow for separation and concentration, and the sampling flow rate is not limited; for example, a sampler capable of separating particles based on the principle of virtual collision and concentrating particles of a target particle size.
In one embodiment, the second diversion holes of the second diversion plate 115 are located right below the recess of the first-stage impact plate 14, so that after the particles pass through the through holes of the first-stage impact plate 114, larger particles (for example, particles larger than 10 μm) directly impact the second-stage diversion plate 115 under the action of inertia and are retained, and the smaller particles pass through the second diversion holes of the second-stage diversion plate 115 along with the airflow, so that large particles which are not blocked on the first-stage impact plate 114 can be effectively removed, and the purity of the collected target particles of 0.5-10 μm is improved.
In one embodiment, the diameter of the through holes of the first-stage impact plate 114 is larger than the diameter of the first flow guide holes of the first-stage flow guide plate 113, and the diameter of the second flow guide holes is smaller than the diameter of the first flow guide holes.
In a preferred embodiment, the target particle separating and concentrating device 11, the sample collector 12 and the exhaust fan 13 are integrally disposed in a cabinet 10, and a cabinet door is left on the cabinet 10 to facilitate replacement of the sample collector 12 and the pipeline connecting the components, thereby avoiding cross contamination.
In one embodiment, the ultrasonic cracking reaction cell 22 comprises an ultrasonic cracking tank 221, a cracking reaction cell 222 inserted in the ultrasonic cracking tank, and a light-transmitting hole 223 opened on the side wall of the ultrasonic cracking tank 221, wherein a cracking agent is placed in the cracking reaction cell 222; the light receiving member 31 collects the light intensity of the lysis reaction cell 222 through the light transmitting hole 223. When the ultrasonic lysis tank is used, ultrasonic waves are arranged in the ultrasonic lysis tank 221, the lysis reaction tank 222 is inserted into the ultrasonic lysis tank, the bacteria lysis degree in the lysis reaction tank 222 can be improved by utilizing the ultrasonic effect, the lysis time is shortened, and the working efficiency is improved.
In a preferred embodiment, the ultrasonic lysis tank 221 is a tank body made of metal or plastic, and the volume of the tank body can be 15ml to 30 ml.
In a preferred embodiment, the quantitative pipette 21 and the ultrasonic cracking reaction tank 22 are integrally disposed in a cabinet 20, and a cabinet door is left on the cabinet 20 to facilitate replacement of the ultrasonic cracking reaction tank 22 and the pipelines connected between the components, thereby avoiding cross contamination.
In one embodiment, the light receiving assembly 31 includes a photomultiplier tube 311 for collecting the light intensity in the ultrasonic lysis reactor 22, and the photomultiplier tube 311 is connected to the signal processing device 32.
In a preferred embodiment, a power pump (not shown) is disposed on the third line between the exhaust fan 13 and the target particle separating and concentrating unit 11 to facilitate the control of the sampling flow rate.
In a preferred embodiment, the sample collector 12 may be a cylindrical or other shaped collection vial with 20ml of liquid sampling medium therein.
In a preferred embodiment, the particle size range of the target particles is 0.5 μm < D < 10 μm, the ultimate concentration sampling flow rate is 5L/min-15L/min, and the flow rate of the gas flow carrying the target particles between the target particle separation and concentration system 1 and the sample transportation cracking system 2 is 5L/min-15L/min.
In a preferred embodiment, the signal processing device 32 is provided with a serial port and a USB interface connected to a computer for deriving the data of the sampling detection.
In a preferred embodiment, the present invention further comprises an electronic control system for controlling the operation of the target particle separation and concentration system 1, the sample transport lysis system 2 and the bioluminescence detection system 3. So as to facilitate the unified regulation and control of each system.
The present invention has been described with reference to the above embodiments, and the structure, arrangement, and connection of the respective members may be changed. On the basis of the technical scheme of the invention, the improvement or equivalent transformation of the individual components according to the principle of the invention is not excluded from the protection scope of the invention.
Claims (10)
1. An apparatus for rapidly evaluating the air bacterial pollution state, comprising:
the target particle separation and concentration system is used for separating target particles in the sampling airflow and carrying out concentration and liquefaction treatment;
the sample conveying and cracking system comprises a quantitative liquid transfer device connected with the target ion particle separation and concentration system and an ultrasonic cracking reaction tank connected with the quantitative liquid transfer device through a pipeline, wherein the quantitative liquid transfer device is used for conveying a liquefied sample into the ultrasonic cracking reaction tank, and the liquefied sample is subjected to cracking and luminous reaction in the ultrasonic cracking reaction tank;
the bioluminescence detection system comprises a light receiving component for collecting the light intensity of the ultrasonic lysis reaction tank and a signal processing device connected with the light receiving component, wherein the signal processing device is used for converting a light intensity signal transmitted by the light receiving component into a target particle concentration.
2. The apparatus for rapidly evaluating the air bacterial pollution state according to claim 1, wherein the target particle separating and concentrating system comprises:
the target particle separation concentrator is used for carrying out separation and concentration treatment on the sampling airflow;
a sample collector, wherein a liquid medium is placed in the sample collector, the sample collector is connected with the target particle separation concentrator through a first pipeline, one end of the first pipeline is communicated with the target particle separation concentrator, the other end of the first pipeline extends into the liquid medium of the sample collector, the sample collector is connected with the quantitative liquid transfer device through a second pipeline, one end of the second pipeline extends into the liquid medium of the sample collector, and the other end of the second pipeline is communicated with the quantitative liquid transfer device;
and the exhaust fan is respectively connected with the target particle separation concentrator and the sample collector through a third pipeline.
3. The apparatus for rapidly evaluating the air bacterial contamination state according to claim 2, wherein:
the target particle separation concentrator comprises a top cover, an air inlet cover, a first-stage guide plate, a first-stage impact plate, a second-stage guide plate, a sampling cavity, a second-stage collection plate, a second-stage exhaust hole plate, an exhaust port, a bottom plate and a sample collection port;
a sampling port is reserved at the top of the air inlet cover, the top cover is connected to the sampling port of the air inlet cover, the first-stage guide plate and the second-stage impact plate are arranged in the air inlet cover at intervals up and down, the bottom of the air inlet cover is communicated with the top of the sampling cavity, a plurality of first guide holes are formed in the first-stage guide plate, a recess is formed in the top surface of the second-stage impact plate which is positioned right below the first guide holes, and a plurality of through holes are formed in the second-stage impact plate which is positioned outside the recess;
the second guide plate and the second exhaust hole plate are arranged in the sampling cavity at intervals up and down, and a plurality of second guide holes are formed in the second guide plate; the second-stage collection cavity is arranged between the second-stage guide plate and the second-stage exhaust hole plate, an annular gap is reserved between the second-stage collection cavity and the sampling cavity, the second-stage collection cavity comprises a collection cavity section, a conical cavity section and a straight cylinder cavity section which are sequentially communicated from top to bottom, the straight cylinder cavity section is fixed on the second-stage exhaust hole plate, and the lower part of the straight cylinder cavity section penetrates through the second-stage exhaust hole plate; the second-stage collecting plate is arranged in the collecting cavity section, a plurality of collecting holes are formed in the second-stage collecting plate, and the collecting holes are in one-to-one correspondence with the second diversion holes vertically;
the exhaust port is vertically arranged on the side wall of the sampling cavity, which is positioned on the second-stage exhaust hole plate in a penetrating manner; the bottom plate is fixedly arranged at the bottom of the sampling cavity; the sample collecting port is fixedly arranged on the bottom plate, and the upper part of the sample collecting port extends into the sampling cavity and corresponds to the lower part of the straight cylinder cavity section; the air outlet is connected with the exhaust fan through a third pipeline, and the lower part of the sample collecting port is connected with the sample collector through a first pipeline.
4. The apparatus for rapidly evaluating the air bacterial contamination state according to claim 3, wherein:
the second guide holes in the second guide plate are positioned right below the depressions in the first-stage impact plate;
the aperture of the through hole on the first-stage impact plate is larger than that of the first flow guide hole on the first-stage flow guide plate, and the aperture of the second flow guide hole is smaller than that of the first flow guide hole.
5. The apparatus for rapidly evaluating the air bacterial contamination state according to claim 1, wherein:
the ultrasonic cracking reaction tank comprises an ultrasonic cracking groove, a cracking reaction tank inserted in the ultrasonic cracking groove and a light hole formed in the side wall of the ultrasonic cracking groove, and a cracking agent is placed in the cracking reaction tank; the light receiving assembly collects the light intensity of the lysis reaction tank through the light hole.
6. The apparatus for rapidly evaluating the air bacterial contamination state according to claim 5, wherein:
the ultrasonic cracking tank is a tank body made of metal or plastic, and the volume of the ultrasonic cracking tank is 15 ml-30 ml.
7. The apparatus for rapidly evaluating the air bacterial contamination state according to claim 1, wherein:
the light receiving assembly comprises a photomultiplier tube used for collecting light intensity in the ultrasonic cracking reaction tank, and the photomultiplier tube is connected with the signal processing device.
8. The apparatus for rapidly evaluating the air bacterial contamination state according to claim 1, wherein: the signal processing device is provided with a serial port and a USB interface which are connected with a computer and used for exporting data of sampling detection;
the device also comprises an electric control system for controlling the operation of the target particle separation and concentration system, the sample conveying and cracking system and the bioluminescence detection system.
9. The apparatus for rapidly evaluating the air bacterial contamination state according to claim 2, wherein:
the target particle separation concentrator, the sample collector and the exhaust fan are integrally arranged in a first cabinet body, and a first cabinet door is reserved on the first cabinet body;
a power pump is arranged on a third pipeline between the exhaust fan and the target particle separation concentrator;
the sample collector adopts a collecting bottle, and the liquid medium in the collecting bottle is 20 ml.
10. The apparatus for rapidly evaluating the air bacterial contamination state according to claim 1, wherein:
the quantitative pipettor and the ultrasonic cracking reaction tank are integrally arranged in the second cabinet body, and a second cabinet door is reserved on the second cabinet body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010433115.5A CN111458327A (en) | 2020-05-20 | 2020-05-20 | Device for rapidly evaluating air bacterial pollution state |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010433115.5A CN111458327A (en) | 2020-05-20 | 2020-05-20 | Device for rapidly evaluating air bacterial pollution state |
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| CN111458327A true CN111458327A (en) | 2020-07-28 |
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| CN202010433115.5A Pending CN111458327A (en) | 2020-05-20 | 2020-05-20 | Device for rapidly evaluating air bacterial pollution state |
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2020
- 2020-05-20 CN CN202010433115.5A patent/CN111458327A/en active Pending
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