CN111455041A - Probe combination for gene screening and application thereof - Google Patents

Abstract

A probe combination for gene screening and application thereof. The invention belongs to the field of biomedicine, and provides a probe combination and application thereof. The probe combination can specifically recognize the pathogenic genes of thalassemia, phenylketonuria, hepatolenticular degeneration, tuberous sclerosis, tetrahydrobiopterin deficiency, hereditary deafness, gaucher disease, amyotrophic lateral sclerosis, methyl malonic acidemia, Usher syndrome, albinism, pulmonary cystic fibrosis, citrullinemia type I, X-linked congenital adrenal insufficiency, Fabry disease, ichthyosis, familial hypercholesterolemia, hemophilia, osteogenesis imperfecta (brittle bone disease) and familial hemophilus lymphohistiocytosis. The invention also provides a corresponding detection kit which is used for quickly and simply screening the population and diagnosing the prenatal period.

Description

Probe combination for gene screening and application thereof

Technical Field

The invention belongs to the field of biomedicine, and relates to a probe combination for screening multiple monogenic diseases and application thereof.

Background

China is one of the countries with high birth defects. According to statistics, the total incidence rate of birth defects in China is about 5%, including chromosome abnormality (0.5%), monogenic disease (1.8%) and polygenic disease (2.6%). Birth defects can be prevented by "three lines of defense" at present: first-degree prevention-pre-pregnancy consultation and examination; secondary prevention-prenatal examination; third-level prevention-neonatal disease screening.

Monogenic disease refers to a disease controlled by a pair of alleles, i.e., a disease transmitted in mendelian fashion. According to the World Health Organization (WHO) statistics, the incidence of all monogenic diseases in the world's birth population is about 10/1000. Except for a few diseases, the monogenic diseases mostly have no cure method, the treatment and nursing cost is high, and the heavy economic burden is brought to the society and the family. Screening of high-risk population before pregnancy, before birth and after birth is a main method for preventing monogenic diseases, planning reproduction and carrying out clinical intervention in advance. And (3) carrying out common genetic disease related gene mutation detection on the couple of childbearing age before pregnancy, and deciding the optimal birth plan according to the detection result and the information provided by genetic consultation. Detecting and diagnosing whether the embryo or the fetus has diseases before delivery, mastering the opportunity, and selecting proper time for intrauterine treatment of the curable diseases; for non-treatable diseases, informed selection can be made. The disease screening of the newborn enables the infant to be diagnosed and treated early, and growth, intellectual development disorder and even death caused by brain, liver, kidney and other damages are avoided.

At present, clinically, for the detection of the single-gene diseases, the traditional detection methods such as low-density chip, Realtime PCR, M L PA and Sanger sequencing are mostly adopted, the products generally mainly adopt hot spot mutation detection, the detected diseases are limited, the detection methods have the defects of low flux, limited detection range and the like, products capable of simultaneously detecting various genetic diseases are also available on the market, and for example, the products adopt full exome sequencing or large Panel (hundreds of thousands of genetic diseases) combined with a second-generation sequencing method, the products are relatively wide in disease coverage, but have the defects of overlarge sequencing data, high cost, insufficient sequencing gene coverage depth, detection omission and the like.

Disclosure of Invention

The invention aims to provide a novel probe combination for detecting multiple common genetic diseases and application thereof, overcomes the defects and shortcomings of the prior art, and provides a novel solution for noninvasive prenatal monogenic diseases detection and monogenic diseases screening of people.

The invention provides a probe combination, wherein the probe is used for identifying the following pathogenic genes: thalassemia, phenylketonuria, hepatolenticular degeneration, tuberous sclerosis, tetrahydrobiopterin deficiency, hereditary deafness, gaucher's disease, amyotrophic lateral sclerosis, methylmalonic acidemia, Usher syndrome, citrullinemia type I, X-linked congenital adrenal insufficiency, Fabry's disease, bullous ichthyosis, familial hypercholesterolemia, hemophilia, and familial hemophilus lymphohistiocytosis.

The pathogenic gene is one or more of ASS1, ATP7B, GBA, GCH1, G L A, HBB, KRT1, KRT10, F L G, STS, MUT, MMAA, MMAB, MMACHC, NR0B L, PAH, PCBD L, PRF L, PTS, QDPR, SOD L, STX L, UNC 13L, TSC L, TSC L, USH 1L, F L, F L, TYR, S L C45A L, OCA L, CFTR, USH 2L, CDH L, PCDH L, USH 1L, USH 1L, MYO 7L, CO L A L or VAPB.

The probe recognizes a protein coding region (CDS) and a splice site region of the above pathogenic gene.

As shown in a preferred embodiment of the present invention, the probe combination comprises a mixture of probes having sequences shown in SEQ ID NO1-SEQ ID NO 378.

The probe can be artificially synthesized based on the nucleotide sequence or obtained by a conventional method in the art.

Correspondingly, the invention provides a gene detection kit, which contains the probe combination.

Preferably, the gene detection kit further comprises negative and positive controls for detecting the gene. The kit can also comprise common reagents, basic consumables, tools, instructions and the like for gene detection.

In another aspect, the invention provides the use of the probe combination for preparing a detection reagent for the following diseases: thalassemia, phenylketonuria, hepatolenticular degeneration, tuberous sclerosis, tetrahydrobiopterin deficiency, hereditary deafness, gaucher's disease, amyotrophic lateral sclerosis, methylmalonic acidemia, Usher syndrome, albinism, pulmonary cystic fibrosis, citrullinemia type I, X-linked congenital adrenal insufficiency, Fabry's disease, ichthyosis, familial hypercholesterolemia, hemophilia, osteogenesis imperfecta (fragility) and familial hemophilus lymphohistiocytosis.

The probe combination of the present invention can be used for recognizing or binding to the causative gene of the above-mentioned diseases.

Preferably, the probe combination of the invention can be used for screening or detecting the related monogenic diseases of the population.

The probe combination of the invention can be used for detecting the variation of the related gene of the monogenic disease, and the detection comprises the following steps:

(1) obtaining separated and purified genome DNA;

(2) adding the probe combination of the invention;

(3) analyzing and detecting the variation of the target gene in the sample.

The genomic DNA is typically taken from a sample ex vivo, such as blood, saliva, hair, etc.

Genomic DNA of a sample is typically subjected to DNA fragmentation library construction and target gene capture prior to detection.

The gene variation detection of the present invention requires a bioinformatic analysis. For example, in a preferred embodiment of the present invention, a blood sample from a breeding couple is used to detect a pathogenic mutation in the ichthyosis KRT10 gene.

The invention discloses a probe combination for detecting common monogenic diseases based on a high-throughput sequencing technology and application thereof. The combination of probes was based on common monogenic disorders including: thalassemia, phenylketonuria, hepatolenticular degeneration, tuberous sclerosis, tetrahydrobiopterin deficiency, hereditary deafness, gaucher disease, amyotrophic lateral sclerosis, methylmalonic acidemia, Usher syndrome, citrullinemia type I, X-linked congenital adrenal insufficiency, Fabry disease, bullous fish scale disease, familial hypercholesterolemia, hemophilia and familial hemophilus lymphohistiocytosis, for a total of 20 disease-associated 39 pathogenic gene-designed capture probes. The probe combination used in the application comprises all core coding sequences and splice junction regions of genes in design, can be used for detecting 20 genetic diseases, and is suitable for population screening and detection.

The invention has the advantages that:

(1) the invention can detect a plurality of common monogenic genetic diseases, while the traditional product mainly detects or screens single disease species, and the detected diseases are limited;

(2) the invention can realize the detection of the whole exon coding sequence and the splicing region of the pathogenic gene, the coverage area is more comprehensive, and the traditional product is mainly used for detecting partial hot spot mutation sites on the gene, the coverage area is narrower, and the omission detection is easy;

(3) the probe designed by the invention has a certain base matching fault tolerance rate, can realize higher capture efficiency and coverage degree, and can detect single-point mutation, insertion deletion, small fragment repetition, deletion and the like.

Detailed Description

The technical solutions in the embodiments of the present application will be described clearly and completely below, and it should be understood that the described embodiments are only a part of the embodiments of the present application, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

Example 1 detection of target Gene Using Probe combination

A. Library construction and on-machine sequencing

1. Sample DNA preparation

The sample can be blood, saliva or oral cavity cast-off cells for extracting genome DNA;

2. sample DNA fragmentation

Approximately 50ng of genomic DNA and/or fetal cell WGA product was sonicated (covaris) to generate DNA fragments predominantly at 200 and 250bp in length.

3. Genomic library construction using KAPA reagents

The library can be constructed by using a commercialized reagent KAPA Hyper Prep Kit (KAPA, KK8504), and the main steps comprise: and (3) carrying out end repair and tail A addition on the fragmented DNA fragments, connecting and purifying adapters (the adapters contain Index sequences, the adapters contain adapters with different Index sequences for connection, and the adapter sequences are T protruding ends), and finally carrying out PCR amplification and purification to obtain a genome DNA library.

4. Capture hybridization Using IDT reagent

After mixing a plurality of (generally 8-12) genomic DNA libraries, 1.5. mu.g of the mixed genomic DNA libraries are captured by hybridization by using the capture probe set of the invention, and the main steps comprise: blocking a genomic DNA library before hybridization by using Hyb Block (IDT), then carrying out hybridization capture on the genomic library by using a capture probe set, enriching a captured target sequence by using streptavidin-labeled magnetic beads, carrying out PCR amplification on the enriched target sequence, and purifying to obtain a target gene library.

The genes capturable by the capture probe set (liquid phase capture probe) include: the synthesis of 41 disease-causing gene probes for 20 monogenic genetic diseases was synthesized by IDT corporation and labeled with biotin.

5. Sequencing on machine

The libraries were mixed at equimolar concentrations and double-ended sequencing was performed using the Illumina sequencing platform, while tag sequences on the samples were also sequenced, NextSeq500 sequencing was performed using the 1.8pM library, the sequencing data preferably being >200 × average depth.

B. Data analysis

1. Sequencing data quality control and filtering: and performing quality control and filtration on each sample original data obtained by sequencing by adopting fastp software, wherein the filtration conditions comprise: removing reads shorter than 75bp, removing reads with N more than 6, and removing reads with low-quality base ratio more than 40%;

2. sequence alignment, mutation site analysis and SNP reading: alignment of filtered double-ended sequencing reads to the human genome (hg19, GRCh37) using BWA software (0.7.15), removal of PCR repeats (duplicates) and non-uniquely positioned reads using Picard software, while filtering out low quality reads (alignment quality <10, base quality <15), and mutation site analysis of gene coding regions using GATK software.

Example 2 detection of ichthyosis Gene

1. Collecting samples: a small blood sample of a couple of child-bearing age (male with suspected bullous ichthyosiform erythroderma and family history, female with normal skin) was collected 1 and subjected to extraction of genomic DNA.

2. Library construction and on-machine sequencing was performed using the probe combinations of the invention and data analysis was performed as in example 1.

3. The data analysis results were as follows:

(1) and (3) data quality control:

the coverage rate of the coding regions of 41 gene regions detected by the probe combination is 100%, wherein the genes comprise ichthyosis related genes F L G, KRT1, KRT10 and the like.

(2) Detection of pathogenic gene loci:

the male detects 1 site pathogenic or possibly pathogenic heterozygous mutation of KRT10 and F L G, wherein KRT10p, L161S missense mutation is pathogenic mutation of bullous ichthyosis (autosomal dominant inheritance), F L G p.K4022X nonsense mutation, and ichthyosis vulgaris (autosomal incomplete exon) is possibly pathogenic mutation, while the female detects 1F L Gp. L ys4022Ter possibly pathogenic heterozygous mutation and does not detect KRT10 pathogenic mutation.

Example 3 Gene testing for familial hypercholesterolemia

Peripheral blood of a plurality of individuals is collected, extracted with genomic DNA, and subjected to library construction and on-board sequencing by using the probe combination of the present invention according to the method of example 1, and subjected to data analysis, gene mutation detection and screening of pathogenic genes related to thalassemia, phenylketonuria, hepatolenticular degeneration, tuberous sclerosis, tetrahydrobiopterin deletion, hereditary deafness, gaucher disease, amyotrophic lateral sclerosis, methyl malonic acid syndrome, Usher syndrome, citrullinemia type I, X-linked congenital adrenal insufficiency, Fabry disease, bullous ichthyosis, familial hypercholesterolemia, hemophilia and familial hemophagocytosis lymphocytosis, wherein 1 of the neonatal familial hypercholesterolemia-related genes L D L R is found to have pathogenic mutation, wherein the pathogenic mutation is L D L R NM-000527.4 C.9G > A (p.AaO7T) missense mutation, the result is verified by Sanger sequencing, the result is verified to be consistent with the parental DNA 6229, and the like, thus the clinical diagnosis of hypercholesterolemia, early-stage diagnosis, early stage diagnosis, and so on the like by the probe combination of the probe combination.

The above description is only for the specific embodiments of the present application, but the scope of the present application is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present disclosure should be covered within the scope of the present application. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.

The probe combination related by the invention comprises one or a plurality of probes with the following sequences:

SEQUENCE LISTING

<110> and Zodiac Biotech (Shanghai) Co., Ltd

<120> probe combination for gene screening and application thereof

<130>

<160>378

<170>PatentIn version 3.5

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<211>121

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<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>1

ggcagaatcc agatgctcaa ggcccttcat aatatccccc agtttagtag ttggacttag 60

ggaacaaagg aacctttaat agaaattgga cagcaagaaa gcgagcttag tgatacttgt 120

g 121

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<212>DNA

<213>Artificial Sequence

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gcattagcca caccagccac cactttctga taggcagcct gcactggtgg ggtgaattct 60

ttgccaaagt gatgggccag cacacagacc agcacgttgc ccaggagctg tgggaggaag 120

a 121

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<213>Artificial Sequence

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attctttgcc aaagtgatgg gccagcacac agaccagcac gttgcccagg agctgtggga 60

ggaagataag aggtatgaac atgattagca aaagggccta gcttggactc agaataatcc 120

a 121

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<212>DNA

<213>Artificial Sequence

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cgtctgtttc ccattctaaa ctgtaccctg ttacttatcc ccttcctatg acatgaactt 60

aaccatagaa aagaagggga aagaaaacat caagcgtccc atagactcac cctgaagttc 120

t 121

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<212>DNA

<213>Artificial Sequence

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tgaagttctc aggatccacg tgcagcttgt cacagtgcag ctcactcagt gtggcaaagg 60

tgcccttgag gttgtccagg tgagccaggc catcactaaa ggcaccgagc actttcttgc 120

c 121

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<212>DNA

<213>Artificial Sequence

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ccttcacctt agggttgccc ataacagcat caggagtgga cagatcccca aaggactcaa 60

agaacctctg ggtccaaggg tagaccacca gcagcctaag ggtgggaaaa tagaccaata 120

g 121

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<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>7

tcaaagaacc tctgggtcca agggtagacc accagcagcc taagggtggg aaaatagacc 60

aataggcaga gagagtcagt gcctatcaga aacccaagag tcttctctgt ctccacatgc 120

c 121

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<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

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ctatcagaaa cccaagagtc ttctctgtct ccacatgccc agtttctatt ggtctcctta 60

aacctgtctt gtaaccttga taccaacctg cccagggcct caccaccaac ttcatccacg 120

t 121

<210>9

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

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cacgttcacc ttgccccaca gggcagtaac ggcagacttc tcctcaggag tcagatgcac 60

catggtgtct gtttgaggtt gctagtgaac acagttgtgt cagaagcaaa tgtaagcaat 120

a 121

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<212>DNA

<213>Artificial Sequence

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<223> Artificial sequence

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taagctgaga tcgtgagctg aagattgaaa agtcccggga aaaaaaaaag tgagtgactg 60

cccttccaag agaaagcaac tgccctccac ctcatgtttt aacagtaatg tagatctgag 120

c 121

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<212>DNA

<213>Artificial Sequence

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<223> Artificial sequence

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cccaggacag gggccttggc tcagcacaga gcatgtgggc tcagtggttt ctgttttccc 60

atcttgacaa atctgggcag cctgcctcct ggttcttacc tgacaaagat ctcagagcat 120

c 121

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<212>DNA

<213>Artificial Sequence

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aaaacgagga aactctgaaa aggataagat gactcagtct ctctccctct tcccgtagga 60

gggtacctcc gtctctatgt caaacagatg tggggctaat tcttaacgag ctccagggaa 120

g 121

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<212>DNA

<213>Artificial Sequence

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agtggtttct ttcaccatcc accatcttct gccggtgagc aggctgaggc cagtgtgtga 60

gggagcagga aggagcccgg agatgtgtgc agcttgggca gctaccactt ctctgcctcc 120

t 121

<210>14

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<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>14

tttttaagct gacagatttg tctccaccat tcttctcttc tctgagctct ttgatgctta 60

tgaccttgtt tccagtcaca tatttggggc attcagccag gaggtcctgc tgggtatccc 120

a 121

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<212>DNA

<213>Artificial Sequence

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gccttatctt tcctgagatg acatagtttc agtctgtggt cactaggcag ggagctcact 60

caagttaata gcgttaaagg tgtcagagca gtattaagga cttggaaaat gaagaaacac 120

c 121

<210>16

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

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tgaggacctc gagaccccac cataggttgt ggcaacttgc aattcatttt ggtctgggga 60

gaggagcccc cggttatctg tggaggaaga gacagaaaga aacagatctc acatgaggcc 120

c 121

<210>17

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

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cccccccgcc aggctggaca ctcacagctc tgtgtcctcc agggccggcg ggcgctccat 60

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g 121

<210>18

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<212>DNA

<213>Artificial Sequence

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<223> Artificial sequence

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gacgctgatg ctgcggaggt cgaggtcaga gcacagcatc aaagcctcga ggtcgatctt 60

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c 121

<210>19

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<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

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ggccaggaag gtctccagcg ggctagtctc gggctccagg tcctcgtcca agcctaaatc 60

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c 121

<210>20

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

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aggctgtcat cgtccaggct gggggagctc tgcagccgac cccgcggcgc tcccacccca 60

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<212>DNA

<213>Artificial Sequence

<220>

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c 121

<210>22

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

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cgggagacgc tgtcctcgtc cgagaggaac atgtcccgga gcggggctcg gccccactcc 60

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g 121

<210>23

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>23

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c 121

<210>24

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

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t 121

<210>25

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>25

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c 121

<210>26

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>26

gcttacccac cagcttgggg ttgaggctgc tctgcttggc cgcgatggag tccaggtagc 60

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c 121

<210>27

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>27

cgttgtctag gcaccagatg ttggctccga aggacaccag gaaggacagg cagtgcaagt 60

ggccattgga agctgccaga tgcaggggtg tgttgcccca gatgtcacac ttgtccgggt 120

c 121

<210>28

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>28

cctcaagggc actgggtggg gcgtactcac ccgcggctca caatgagacg cagcgactcg 60

aggttgccat ggtaggcagc ccagagagtg ggggtcatgc catcctcgtc gggggcattc 120

a 121

<210>29

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>29

ccatggtagg cagcccagag agtgggggtc atgccatcct cgtcgggggc attcagctcc 60

tttcgggtgg cctccttgag gagctccagg tagccatccc gggctgcccg gtggtactgg 120

t 121

<210>30

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>30

agaaagttta ttttaaaata ttttacactc ttttgcccac agctctttat tcttccctca 60

tggtgaactg cactactgca cttgtgtggc ccacatgact ttatatcttt gtacagagca 120

t 121

<210>31

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>31

atttccagca tcatatcatc catgctgact gtgccgatga tgggcctgaa gaacagttca 60

gcaatgacat tggcattgat gaatctcagc aggaaaaggg tactattaag ttcgatgaat 120

c 121

<210>32

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>32

tctgtcatgg ggcccttggt gcgtcatcct ggtgtgttca ctgagtattt gctgagttcc 60

ccactggagt ccctgaatgt acttcacgca ctgcaggccc ggcacgtctg gagggagaaa 120

a 121

<210>33

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>33

actggagtcc ctgaatgtac ttcacgcact gcaggcccgg cacgtctgga gggagaaaaa 60

tctctttgtt ataaaacagc tcaccacaga gtcctttgct agctttttaa aaatagccat 120

t 121

<210>34

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>34

aaaactcgtt actgcccgcg cccctagata ggcactgccc gatgcttttg tgagctggga 60

aaagccggcg cctaaggcca gtacccttac ccgggttaaa gagcacggtc cccttgaggt 120

a 121

<210>35

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>35

tgaggtaggc gtactccttg gtactgatgt tcagactcca gcatttggaa agaaagcact 60

tgatggcttg gacctgggag gcggagggca ccttcctggc ctccgccggc ggtgccaaat 120

g 121

<210>36

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>36

agcgtgggca cgggcagtgg ctcgttgccc ccggtctccc gccgcctggt ggtgaggatc 60

ttctgcagca tgctgggctc cgagacttcc acagtctcga actgcaagcg gtcctgggcc 120

a 121

<210>37

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>37

gccagctcaa gcatgagcag ggacgcccag cagttgcgca ccagcaccag ctgctggtcc 60

aggggcagca cctggaagca gggcaagtac ttgacgaagc gcagcgtctt caacaggccc 120

g 121

<210>38

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>38

gaggctgcct cgcagaccac ctgtggactc ttgagcgcca ccggccgcag cgcaccagag 60

gaggtgtccc accagggggc cctcggccgc tcctccggag ccgcctgcgc ttgatttgtg 120

c 121

<210>39

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>39

ctcgtgggca cgcagtagag ggtgctgccc tgctgcgggt ggtcttcacc gcaaaagcag 60

cagcggtaca gaagcgccgt ggcccgcccg cctggtagcg cctctttacc ccctggcctc 120

t 121

<210>40

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>40

gcctctgcgc gaagtaggag cggtcccacc acgcgccccc tggccgtgcc tcgggcgctg 60

ccggagccac gtgcgtttgc tttgagctag tgagcaagct gtagaggatg ctgccctgcc 120

g 121

<210>41

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>41

ccgcgggtgg tcttcaccac aaaagcagca gcggtacagg agtgccacgg gccgcccacc 60

cggaagcccc gctctgccca ccccgggatc agagccgcac gaacagcccc agcacggacc 120

c 121

<210>42

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>42

cagcgtcgcc tcgggcgcct tcggtgccgc gtacgtttgc tttgcgctcg tcagcatgct 60

gtagaggatg ctgccctgcc gtgggtggtc tttaccgcaa aagcagcagc ggtacaggag 120

c 121

<210>43

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>43

ttccgcccgc ccagcagccc ctctctgccc accccgggct catcgccgca cgaacagccc 60

cagcactgat ccaccagccg cgtctctgga gcctcaggag ccgcgcgcgt ttgcttcgcg 120

c 121

<210>44

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>44

gcgctcataa gcatgttgta gaggatgctg ccctgccact ggtggttctc gcccgccatg 60

gcccgcggcg cccgtagccc agttctgccc agtggctgcc tcctgggacc tatttatacc 120

t 121

<210>45

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>45

caattcctac ttcatggatg tgaaataccc aggatgctat aaaatcacca tggtctttag 60

catgcacaaa cagtagtttt gtgttttggc tgctctactg tccactgcta gcccacaaga 120

g 121

<210>46

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>46

aggaaaagca aggcttacag aaggatgttc ctttaggaga aagcagcact aaaagcactc 60

tgaatcaaga tgagtgggaa accatctcaa taagcacatt ttggattaaa aaaaaaatta 120

g 121

<210>47

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>47

aaggctgtta tttcaaatta aggtttgctt ttcggacttt ttctgatgaa agaaatagtt 60

ggatctccat caacagattc acagctgaca gaccacattc tgtccatggc tttactttat 120

t 121

<210>48

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>48

actttatttt ctggagggca ctgcaaagga ttccaatttc acctacaaag aaaaacacca 60

tcaaaatggg ccacttgtat aataagcaaa aactcttcaa gtgtcctcag tgcaaagcac 120

t 121

<210>49

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>49

attaaatatt tcaataaatt tattattttt cctatggcga tggtagggaa agacagtctt 60

cgattactga gaaaccgagt ggcctcgtaa ggtgtaaatt acttactgtt aatggaatca 120

g 121

<210>50

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>50

atcagccaaa atcttaagct gctgggtatt gtccaagacc tcaatccttt gggtgtatgg 60

gtcgtagcga actgagaagg gccgaggtat tgtggcagca aagttcctaa gaccaaaacc 120

a 121

<210>51

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>51

agcgaactga gaagggccga ggtattgtgg cagcaaagtt cctaagacca aaaccacagg 60

cttgagtgaa gggcaccatt tggagaaagg tagtcttaag agagttctca gtggcatttt 120

a 121

<210>52

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>52

gctgtagaca ttggagtcca ctctcctggc caaccaccca cagatgagtg gcaccagtca 60

ggaggccccc agagctagtg gctcaccttt gtcaccacct caccttactt tctccttggc 120

a 121

<210>53

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>53

aaactctctg ccacgtaata gaggggctgg aactccgtga cagtgtaatt ttggatggct 60

gtcttctcca gctccagggg gagaagcttt ggcttctctg ataagcagta ctgtaggccc 120

c 121

<210>54

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>54

gctgtcttct ccagctccag ggggagaagc tttggcttct ctgataagca gtactgtagg 60

ccccaagtga aaagttatta tcactgttaa atcaggatca gtattccctg ctgcatccca 120

t 121

<210>55

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>55

agggtttcaa caatattgaa agcacaataa tggttttctg tacccaccac ttttaaatct 60

atccttggtt cctgtgaagg tcatacctgt aattcaccaa aggatgacag gagcccagca 120

c 121

<210>56

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>56

accatatgcc tttatggagt ctccttgttt gcagagccca aactccacag taaaccagta 60

aatctggaat ggaaagtcaa tctgagagca cactctatga tggttaattt tatgtgtcac 120

c 121

<210>57

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>57

ttttttcccc agataacctg gcttccaggg gagtaggaaa gtttcaaaga cctgagggcc 60

atagcctata gcactccacc atccacccag ggagagaagg gacttactgt ggcgagcttt 120

t 121

<210>58

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>58

aatgtattca tcaggtgcac ccagagaggc aaggccaatt tcctgtaatt gggggaaaat 60

agaacctgtt ctgttcctgt aattggaacc acagaaccaa cctagtactt ggccataaaa 120

a 121

<210>59

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>59

tccgctcttg cagagggcat gaggacccct ccctgggctc aactcatttg agaaattcag 60

gtcacagacc tataactaga aggctaaaaa atccattcct tacctgggaa aactgggcaa 120

a 121

<210>60

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>60

agctgcgatc tgaaaacaag ggcacatgtc ccaacagctc atggcagatg tcactgaaag 60

acagaaagca cagagagctc ggaggggagg aggtttaagc caagccagac tcagtctttc 120

t 121

<210>61

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>61

ctgtggacca gccagcaatg aacccaaacc tcattcttgc agcaggaaaa gatggcgctc 60

attgtgcctg gcaactggta gctggaggac agtactcacg gttcgggggt atacatgggc 120

t 121

<210>62

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>62

ggcttggatc catgtctgat gtactgtgtg cagtggaaga ctcggaaggc caggccaccc 60

aagaaatccc gagaggaaag caggccagcc acaggtcgga ggcggaaacc agtgcaagct 120

g 121

<210>63

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>63

aagaaatccc gagaggaaag caggccagcc acaggtcgga ggcggaaacc agtgcaagct 60

gggatgaaaa gaagaaagaa aactcaaagc tcatcaccac tgagtcagag gcactaggag 120

a 121

<210>64

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>64

tcatacttgc ctccacatac ttgtcttccc cttccctctc ctctgcctca atcctccccc 60

aactttctgc agggccattg accctgatgt ggacttactc tgcaggaact gagaaacgtc 120

t 121

<210>65

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>65

gctggggaat gttatcttca tggaagccac agtacttttc aagaagtgga aaaatgtgat 60

tgtactcata gcaagcatgg gttttataca aggacttcag agtcttgaac actgtgcccc 120

a 121

<210>66

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>66

catgttttct tttcttcctc catgtattcc actcgaggga tgggctgccc actagaatac 60

aggcacaaaa taggtgtctc aagcagggca ggggcacagc agaacgcagg ttaggttagc 120

a 121

<210>67

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>67

ttcatgctgg tattttccat cctcaactgg atgagggcaa gggagaagca ggctaggggt 60

gtgtttttct ctcttcccct caacaagcaa ggcagactta ctggcggtag ttgtaggcaa 120

t 121

<210>68

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>68

gtcagcaaac tgcttccgtc ttgcacggta cacaggatct ttaaaaccct aggagaaaag 60

agacacctga tttttcaagg cttcatagga agaggtctgg tacctttatg aatgctttga 120

a 121

<210>69

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>69

gtacattgct ccaagtagag aaggtaagag gaagggaggg gagtggagga gaggcactga 60

aaaaatctca tcctacgggc catggactca cagggtggtc agcatccagt tccgctccat 120

a 121

<210>70

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>70

gaaaaaatct catcctacgg gccatggact cacagggtgg tcagcatcca gttccgctcc 60

atagctgaga atctgattgg caaatctgtc cagctcttga atggttcttg ggaaccaggg 120

c 121

<210>71

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>71

taaatataca tatatttaaa tatgctgtta aatatgtata ttgctgttat tttatgaaga 60

cagtgtggag ttacttatgt tgcaaaattc ctctaattct tacctgtgtc tttcttctta 120

t 121

<210>72

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>72

tatctcgtga aagctcatgg acagtggcac caatgtcatg cctcaagatc ttgatgatgt 60

ttgtcagagc aggcaggcta cgtttatcca aatgggtgaa aaattcatac tcatctttct 120

t 121

<210>73

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>73

ctttaaacga gaaggtctag attcaatgtg ggtcaggttt acatcattct cctagaagag 60

agaatgggga gggtgaggag acagtcactg gaactaacgc aggccaaaga tgcagaacca 120

g 121

<210>74

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>74

ttcaaatctg cctgttccag atcctgtgtt cttttcattg catctaacta gaaaagaaca 60

tggaagtttg ctacgacatt atccaagaca aacatgattg tagcactgac ctcaaataag 120

c 121

<210>75

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>75

ataagcgcaa tactttggcc aatgcaccaa cttcttcttt gagtgagaag atcagtgata 60

tggcaccatt ttgattgcag ttgtcttcaa tatagcttgt ttcctacagg ataagatgca 120

t 121

<210>76

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>76

ttttgattgc agttgtcttc aatatagctt gtttcctaca ggataagatg catttgttta 60

aaacattttc cacagtttag caattcattc ctgttaaacc tccatggaca aagcaagcat 120

g 121

<210>77

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>77

ttcttactga gcttatgaaa ccaggaagca ccagcagtct tcggatctct ttctctggag 60

gcccaaattc ccctaactga gcagctcagg ctgccgtggc tcacctgtcc aaagtcagag 120

a 121

<210>78

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>78

gagtttcctg cccaagcctg ggttttccag gaccgcagtg gacatgctgg ctccccggga 60

gtgaggtctc tggcttttta gggcctcagg tacaggcagg tttgcaaaca gcacgtgggg 120

c 121

<210>79

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>79

caaccataga tgaaagaact ctaccgtcgg gcgccacctc ttcaataatt gtcttgatta 60

ctctggtttt gttagtatct gtgtgaatga tggaaaaaaa attttaaaca gtctgtaggg 120

a 121

<210>80

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>80

ctgattaccc cagctagttt ctgctgacct tggtccctta gatgaagact cgcccacgga 60

cccggaagag gaggacttgt ggcctccgct ggagctgccg ccgccgtatc cgccgccgga 120

g 121

<210>81

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>81

taacttttca ttttaaaatt taccttccct ctccttctag caggctgcgg taggtttgaa 60

tttcattctc cagtcggatc ttaatatcca ggagttgttg gtattcagta ttctggcact 120

c 121

<210>82

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>82

ctggcactcg gtttcagctc gaatctgttg caactgttct tccagagcgg atatctgggc 60

ctgaatctgt gagagctgca cacagtagcg accttctgtt tctgccaagg aggcttccag 120

g 121

<210>83

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>83

atttcatgag agttaacata ccaaggccag ttgggactgt agttctatct ccagagcttg 60

tacattacgt ctcaattcag taatctcaga tttatagctg gatatctgtt caatgttatt 120

a 121

<210>84

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>84

ataccaaggc cagttgggac tgtagttcta tctccagagc ttgtacatta cgtctcaatt 60

cagtaatctc agatttatag ctggatatct gttcaatgtt attatcaatt tctgtagtca 120

g 121

<210>85

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>85

tcactataag gaagattact ttaccttttc attgaaccag gcttcagcat ctttgcggtt 60

ttgttcagca agttgttcat attggcttct catgttattc agaagttgag tcagatcaac 120

a 121

<210>86

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>86

gttttgttca gcaagttgtt catattggct tctcatgtta ttcagaagtt gagtcagatc 60

aacacccggg gcagcattca tttccacatt cacatcacca gtggacacat ttcgaaggtc 120

t 121

<210>87

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>87

ggaaaagtat aacttttgtg tcacctcctc gtggttcttc ttcagatagg ccagctcttc 60

agtcaggctc tcaatttgca tctccaggtc agccttggtc agggtcagct catccagcac 120

c 121

<210>88

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>88

ctcttcagtc aggctctcaa tttgcatctc caggtcagcc ttggtcaggg tcagctcatc 60

cagcacccta cgcaggccgt tgatgtcagc ctccacgctc tggcgcagag ctacctcatt 120

c 121

<210>89

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>89

tgatagtatt atacaacgat cacttaactt acttcagcct gaagtcatca gctgccagcc 60

tggcattgtc gatctgaagc aggatgttgg cattatcagt tgttaggttg agaatctgga 120

g 121

<210>90

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>90

ctggatttaa aaatacctct tacctgattt ttaaggtcat cgatggtttt gtagtatttg 60

ctgtagtcac gaggctcccc ctgatgtgag ttgccatgct tttcatacca ctccttgatt 120

t 121

<210>91

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>91

ccactccttg attttgcctt ccagctcata gtttgattct tccagagccc gaactttgtc 60

caagtaggaa gccaggcggt cattcagatt ctgcatggtt actttttcat ttccagagag 120

a 121

<210>92

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>92

ctgcatggtt actttttcat ttccagagag aaggccacca tctcctccaa atccaccacc 60

aaagcctcct ccaaagccgc ctccaccaaa gccgcctcca ccaaagctgc ccccaccaaa 120

g 121

<210>93

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>93

ccaaagctgc cacctccgaa actgccccct ccaaagatgc ctccataact cccaccaaag 60

ctgctacttc catagcttcc acgaaagcta cctccaccaa aacctcctaa tcctccatag 120

c 121

<210>94

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>94

ctcctaatcc tccatagcca cctgatgagc ccccaaagca gcccccacca gagctcccac 60

ggctaaaaga gccaccactg aacccccctg agctaaatcc tccaccaagg gagcctttgc 120

t 121

<210>95

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>95

ctttgctgct agaaattctt agggatgaca ctcctcctcc tcctccacat cctcctcctc 60

ctcctcctcc tccactgcgg gaggaagagt agtgcttgct tgagctgtat cgaacagaca 120

t 121

<210>96

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>96

tcagcccatc ctgctgctgg ctgtcctgct cagcttgtgg tgagtggagg caagtccctg 60

ccccggcccg cctgcctgaa gtcatcagat gtactgctcc tcatccctgc cattcaggag 120

c 121

<210>97

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>97

ttcaggagca gagaccactt gtccccatca tcgtctgctg cagcgctgtg ccgagatggc 60

ttgtcggacg tcagggagga cagcgacacc tggctgacat agctgacctg gtcccatggt 120

g 121

<210>98

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>98

tgtggccctg ggggagtccc gccacctgtc atccatgcct atgtgcacac tgacctggga 60

tgccgtcagg ggcttcatgt ggccatgcgc ctgtgcctca tacctctcca ggtcaggctt 120

c 121

<210>99

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>99

tgccgtcagg ggcttcatgt ggccatgcgc ctgtgcctca tacctctcca ggtcaggctt 60

cttatagctg gaaagcagga acgcaacagc atctgagcca ttctagaaac aaggcttttt 120

t 121

<210>100

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>100

gtcccaggtg aatgaatggg aaatgagagg caagttccac tgtgctaagc atgcagaatg 60

acaaggcctc ctgggagcct cccacagatg ctccacctga ggggactcac cacttgagct 120

g 121

<210>101

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>101

gctgcaggga tgagagcacc acagacacag aggaggctgc catggccgct gagcccatcc 60

agggctgcag cacaatgccg atgggcatga agacacctgg ggaagaaaga actcgcactc 120

a 121

<210>102

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>102

ccatccaggg ctgcagcaca atgccgatgg gcatgaagac acctggggaa gaaagaactc 60

gcactcacac ctaggtctgg ggagaggagc caggagaggg cttcaggcac cgggctgccc 120

c 121

<210>103

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>103

ctctagccag ccagtgagtg agccactcac taaccccagc aggaacctgg gagacagaag 60

cctttctggg cgcagctgga gcacagtggg taagagctgc ctacctgctg caatgggtat 120

c 121

<210>104

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>104

atcccaacca ggttataaat cagtgccagg accaggttga tgcgtatcct tcggacagtc 60

ctcttggaaa ggtgaatgct agccaccaca tccagcaaat cattctgatg gagaggagca 120

c 121

<210>105

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>105

cttggaaagg tgaatgctag ccaccacatc cagcaaatca ttctgatgga gaggagcaca 60

cagtgaggaa ggggtctgcc cattgccctc ccagcaccca cagcctggct gcagccacgc 120

t 121

<210>106

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>106

catccagcaa atcattctga tggagaggag cacacagtga ggaaggggtc tgcccattgc 60

cctcccagca cccacagcct ggctgcagcc acgctcactc tgataaggac gacgtcggct 120

g 121

<210>107

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>107

gccacatccg tgccggtgcc aatggccaca cccatgtctg cctgggccaa ggccggggag 60

tcattgaccc catcccccac catggcgact ttcttccctt tattctggag ctcctggacc 120

t 121

<210>108

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>108

tggaccttgg ccaccttgtg cgaaggcagc acctctgcaa agactttgtt gatgccaacc 60

taagacaaaa ggaaggcaat gcctagtgtt ggcaaaaggt atcagatagc agcagaaacc 120

t 121

<210>109

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>109

caggagtaca gctcagtgct gggccaactg gtgcttactt ttgtctctaa ctgcttttat 60

gagctttaca cagtttgcaa cattaaaggg ctgtacctgg gtggcaatag ctctggctgt 120

c 121

<210>110

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>110

cttccggttg tcccccgtga tcagaaccac gtccacaccc atgctctgca gcgtgtgcac 60

agccagggca gcctcctgct tgacagcgtc tgcgattgcg atcatcccac agagcacacc 120

t 121

<210>111

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>111

cagggcagcc tcctgcttga cagcgtctgc gattgcgatc atcccacaga gcacacctgg 60

agcgaaccag ccagcatcag cagctacaca agttggggca ccccgcacca agataccaca 120

c 121

<210>112

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>112

cttttctttt ataaaggagg actcttttgc ctgatatctg cagaaaactg tatttctgag 60

agagcggaag gaaggcagaa gcagaagata ccgtcaatag ccaccaggat ggctgtctgt 120

c 121

<210>113

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>113

gtctgtcctt tcatctcgtg gtctgtcata gcgtcactga catcgctaga aatggttaaa 60

ccgttgcgcc tcagccactc acggtttcca atcagcacag agaaggtctg ggggactgca120

t 121

<210>114

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>114

cgcctcagcc actcacggtt tccaatcagc acagagaagg tctgggggac tgcatctatt 60

caaaagaggc tgtggttatt tctaaatggt ccaatttcac tgtgaactaa aaaccatgca 120

a 121

<210>115

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>115

cttagccatg aaccgtctgc cgcacagcag aggcaatcac tgctgggcgt ggtgctctct 60

gtggtttgac ccacctctac ttttaaccag ctgcagagac aaaagccagc aatacctttt 120

t 121

<210>116

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>116

gggaaggctg ccagcctcat tcaggtgact ggccggtgca ctcaaagggc gctcactgtg 60

ggccaggatg ccttccacgt tgctgacttt gcacccaatt ccacagcctg gcactgcctg 120

g 121

<210>117

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>117

ctggaagtcc gtgcagtatc ccaaggtctc tgttccaagt tcctgggaag gtggaaagag 60

aggaagagga aactgtaagc caagaggggt gaagtgaaag ggaggggcag gcaggacagg 120

g 121

<210>118

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>118

acacagagaa ggctcctcga gggcagctag gagagaagga catggtgagg aataaaagag 60

cattggcggg gagggcaggg ccacgcccaa gtccacgtac ctctttacag tatttggtga 120

c 121

<210>119

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>119

actgccacgc ccaaggggtg ttcactgctg gcctccgcag tccccaccac agccagaacc 60

ttcctgaggg gcagtgtggc cacatccccc agcaggagca cccgcatgac cctggggacg 120

c 121

<210>120

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>120

ccatgggtaa tggtgccagt cttgtcaaac atcacagtct ttatctgcca aaaacaacca 60

caactcactg accacaatac agatggaggg gcttccatag tcacactcct gaggcagaac 120

t 121

<210>121

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>121

gtcttgagtg gctctcaggc ttttctctca atgtgaaata gtaaacagat actactttca 60

tctctcagga tggggaaagc cgtgctacag gctgaccttg tgcgccatct ccaggggctt 120

g 121

<210>122

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>122

cctcccttga tgaggatgcc gttctgcgcg gccaccccgg tgcccaccat gacagccgtg 60

ggcgtggcca gccccaggga gcaggggcag gcaatgcaca gcaccgtgat ggacgtctgg 120

a 121

<210>123

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>123

aagcaaaccg gatgatcacc tctgtctggg agatgtgctt gttggggttc tgaaaacagg 60

acagagtcag aggcaggttg agagttcaat aaggaagctc ccagaactct aatcacataa 120

g 121

<210>124

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>124

tgaataatta aagcccagtg aatctaagat atgaaagaac aggatcaatg tcagtagatt 60

atttaaaaca caaccaccat atagcccaag gcattcaact tacaggaaag tatctctgaa 120

c 121

<210>125

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>125

acacaaccac catatagccc aaggcattca acttacagga aagtatctct gaacaacacc 60

aaaatcgata aaaccgatta caatccatac caccaacgtc aaagttgaca tgatgatgat 120

a 121

<210>126

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>126

taaatgggac aaaatatcca ctaaaccggt cagccagctg ctgaatgggt gcctatgaaa 60

ataaaacacc aagaccatgg gaaattacaa cctatgaaga aataaaacac cacaagcatg 120

g 121

<210>127

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>127

tcaataaaac tgtctgattt cccagaactc ttcacataat ttctaaaacg agaaagatga 60

agttagtttt aaaaatttct tcattacctt tgacatctga gcctcttcca ccagtttcac 120

a 121

<210>128

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>128

atctgagcca aagtggtgtc attgcccacg tgggtagctt taatgagcac agagccatgt 60

gcatttatag accccgcaat tacagtgctt ccgggtttct tagtgactgg catggcttct 120

c 121

<210>129

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>129

atttatagac cccgcaatta cagtgcttcc gggtttctta gtgactggca tggcttctcc 60

tagacgtagg aaagagacaa ctgtcacttg ctcagcccca tccagcactc atgtgacctg 120

a 121

<210>130

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>130

gtaggaaaga gacaactgtc acttgctcag ccccatccag cactcatgtg acctgacagc 60

tgctatgata tcctcctgag ggaacatgaa acaagccatc tcacctgtga tgagggactc 120

a 121

<210>131

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>131

tcatcagcca tggtattgcc ttccaggact ttcccatcca ctggaaactt tcccccaggg 60

accaccttga cgatatcgcc ccgctgcacc agctccatgg ggacttgctc ctccctgcaa 120

c 121

<210>132

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>132

gacgatatcg ccccgctgca ccagctccat ggggacttgc tcctccctgc aacaaacgcc 60

acttatcact cacatggcca ctcattcggt caccgggtca ggttctaggc cagctgttac 120

a 121

<210>133

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>133

caatgtcaat acaacatggg catctgatgc agctcacaca gattgataga taccaaccac 60

aaagacattt gataaccata actcacctga tgattaaatt gtcctcacca agggtcacaa 120

c 121

<210>134

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>134

cggtggcttc tgtggcttgg agagacatga gtttagccag ggcttctgag gttttgctct 60

aggaaataac cagaatgtga aatgagagct atcgaaagca gacctgtcca aacccagcct 120

g 121

<210>135

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>135

ctgtctctat gctgtgtata attagtaatt ctaaacatgg tgttcagagg aagtgagatt 60

tgtttactga aggagcagct cttttctgaa cctgaagctg ctgttacctt tgccaagtgt 120

t 121

<210>136

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>136

agtgttccag ccaccggccc agggcaatga acacaaagag catggggggc gtgtcgaaga 60

atgtcacagg gctcctctcc gccttctcag ccacagcaac caccaggatg accagagaat 120

a 121

<210>137

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>137

aacataagca atgcttgtgg ccaggacgat gagcacgtcc atgttggctg acctgtgtct 60

cagagatttg taggcctgaa cgtagaagta ccacccaccg aggagctgaa agacaaggac 120

a 121

<210>138

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>138

gagatttgta ggcctgaacg tagaagtacc acccaccgag gagctgaaag acaaggacag 60

tgaaggctgc cagcaagtag ggaggagagt tcaatgagcg acacagggcc aagggccctg 120

g 121

<210>139

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>139

aagtgccatt taaaccaagc taaagcacta tgtttgcgct tagcgggcag aatatctgag 60

ggccacacac agcatggaag ggagaggtct gcccactttc tcatatatac ctggacaaag 120

g 121

<210>140

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>140

acaagataaa gaagatgaga tttagaatgg acagtcctgg aatgatgttg tggtccagga 60

ccatggactg gtggggctcg ttgctgggta tcagcatata gatcattaag gccatgacag 120

g 121

<210>141

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>141

acagggatgc caaacaccag gctgcacagg aaagacttct tccacctgga aagcaaatgc 60

agcaacacag atatatcaga tgctgcttgt cacctggatt acaagccact ccccttctga 120

g 121

<210>142

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>142

aggcaccatg ggaagacaag accagctggc agagttgggc ccaggtagag gaagggactt 60

agatgagagc tggagtttat cttttgtgtt ctacctactg ctttatttcc atcttgtggt 120

c 121

<210>143

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>143

agtgatgagc gttggggttt ctctgggcca gggaagcatg aaagccaatt tccttgtcat 60

taaaaagaga ggggtgggga aaaaggagga aggtacttgg ttaaaatatg cattggcaga 120

a 121

<210>144

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>144

gaagaatttt ggttattttc attttttctt agctatattt tctcattttt cttcactgat 60

tatatattac tgtttttaaa aaggtgacta caatttttta atgaattact tacctcaata 120

a 121

<210>145

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>145

tttgataata tcccgtggac cgataatttc cgggtcaaac ttaacaaggg ctttgctggt 60

ggcaagggca acggaggcat aagtgatgcc atttgtcctc gtgagtttgg actctatgtt 120

g 121

<210>146

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>146

ctatgttgtg gacacaggac gcgcaggtca tccctgtgat ctgcaacaca ggatggcaag 60

aatcccacag acccaggatc aaggaaagcc tgtgaaagcc agtccaggga gtctaggtaa 120

c 121

<210>147

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>147

aacaacaaca aaccagacac gtccaagatg gggaaattta ctaatcacaa agatggatgt 60

gtccaaaatg caaactgtca gaagcctgta accccgtaac gcacccacag tacttactgt 120

c 121

<210>148

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>148

atgttgccat cggagcctgc gtagtcctcc atgactgctg cctcaaaacc caggtcctgg 60

atgaactgag ctatctcgag gggctggatg acctctgggt catacttgat ctctgccttt 120

c 121

<210>149

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>149

ctttcctgcc atcaaggcaa ccaacacgga gagaacacct ggaaccatca ggtcatggct 60

gtaacactct gggtgggcag ggcctctagg ttgacacagt caatataacc gaacaaagaa 120

a 121

<210>150

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>150

gggctactga taaacacagt tgctgggtat tctgaaggga gaatacgagg tctatacgca 60

gcattcctaa gttcaacatg ggcgttcatc tcttaccagc ttctttctgc agattccttt 120

c 121

<210>151

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>151

tctatgttag acacacagga tgcacaggtc atgcctttga tctgtaagaa gcacttctgc 60

ggtgccactg ctctggttga ttgtggggac tttgccaaga tgtccggggc atggtttgca 120

g 121

<210>152

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>152

gggagcctcc cagtgtgggg agccacttcc tgcacagatg taggtgtacc atctgtagtt 60

tgcaccatgg aattcccagc actgtggttt ccaagagggt tagtagaaca gctttctagg 120

a 121

<210>153

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>153

gcaccatgga attcccagca ctgtggtttc caagagggtt agtagaacag ctttctagga 60

taaaatgtca gaaaatattc aaattagaag agcaaataat atgtttttca gaacaagagg 120

t 121

<210>154

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>154

ctcacctata ccaccatcca ggagctataa gacacaaaga gaaaaggaga caagctcagg 60

acatgcctca aacacactac gtaccagaaa cgactgaagc ctcaaatccc atgtcttcta 120

t 121

<210>155

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>155

tctatagcag ctctgagttc ttctgggcta attacagagg gattataaag aactgttgca 60

gtcccttcgg ccaaagacac cgatatttgc tgcacccctt ccagttggga gatcatgcct 120

t 121

<210>156

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>156

ttcaatggaa tggacacagg atgcacaggt catgccggca atggcaatca gagtggtact 60

gcatgtgccc tggacctggt ttctcggtgg ggagccaggg gaatgagaac tggaagacct 120

g 121

<210>157

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>157

atctgtccca ctcccttcgg ctccatcagg aagagaaact ttaaaattcc caggtggaag 60

tgcctcgata gccctctgca gagccactgg gctggtacaa gaagggtcat actttacttg 120

g 121

<210>158

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>158

cttgggcagt tttgttctcc aaggacactt gaatactttg aacccctagg agctggccaa 60

tattttcttc aatattcaag acgcaagact tacaatgcat tccatctatt ctcagttgga 120

g 121

<210>159

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>159

gagggtgacc acatggcttc cttggtgccc caaggtctca gaattattaa aattctggtt 60

agcagaagat aaaggtctct ttgggttagt gctttgtaac cgctcaatat caattggtcc 120

c 121

<210>160

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>160

cttaagggag ccactttgct cttgatggca gcttcaaatc ccatgtcatt tacatggtcc 60

ctgaggtctt cgggctgaat gagataaggc tgataagtga tgacggcctc ttggttgctg 120

a 121

<210>161

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>161

agactttgac tctcactact ccttgcagtt tccggacctt gccttcaatg gagctgacac 60

aggactggca ggtcatgccc tccacccgga gcttgaccac agcctcctgg gcaggcaagg 120

a 121

<210>162

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>162

aaggaccttg agggccagga ggctgccttt ccttctgcaa tgctggcctc gaagcccatg 60

tccccaattt gatggcaaac ctgttgcagg cacacaaccg atggcacata tttcacagtg 120

g 121

<210>163

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>163

actgccttgt tccagggaaa ccttcatgct gatgatgcct ttcaaattgg aaatcctgtc 60

ctcaatggac ttcacacatg actggcaagt catgcccaag atcctgactg tgctggtggc 120

c 121

<210>164

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>164

ccacctgaga agaagggccc aggccatcca gaccaccttc atagccaaca ttgtcaaaag 60

caaaactctt cttcattgct ggttcccagg cacgggtagg caaagaaagc ttagataaga 120

t 121

<210>165

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>165

aaagcaaaac tcttcttcat tgctggttcc caggcacggg taggcaaaga aagcttagat 60

aagatctaaa aagaaaagaa ataacatttt ttaaccttga aaccaaatat tttctacaac 120

a 121

<210>166

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>166

gctcggcctt ccctgcgcac cccctggggg cgagtaagcg ccgaacgcgg ggaggaaaat 60

cctcctggtg ggagtgagca cgctgcgcgg acgcggggga acaaaactca ctttccgact 120

g 121

<210>167

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>167

ggccccttct ctggctgtga tctgtctctc ctgctcaggc atcgtcccgc acggacaccg 60

aattcttctc tgatctggct cagagcaaaa ggtcacctgg tcggtggagg agagcggggt 120

g 121

<210>168

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>168

ctgtaagctc acactggccc tgctgtgccc tctttagtca cagacagcgt gtgagctgac 60

tctgtccctt taatgcccag gctgagccca gtgcctcctt gagtatctgc tccatcactg 120

g 121

<210>169

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>169

gccacaggta ggtgtgaatg gagtagccag gtgagattgt ctccaggaag cccacagcag 60

gatccttgat ggtaagaggc acatccttag aggagctagg gagcagggag gagaagctga 120

g 121

<210>170

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>170

cacatcctta gaggagctag ggagcaggga ggagaagctg agagtgtgat cctgccaagg 60

cccccaacgc tgtcttcagc ccacttccca gacctcacca ttgccctcac cggtttagca 120

c 121

<210>171

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>171

cacaacagca gagccatcgg gatgcatcag tgccactgcg tccaggtcgt tcttctgact 60

ggcaaccagc cccactctct gggagccctc aggaatgaac ttgctgaatg tgggaacaga 120

t 121

<210>172

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>172

tggcaaccag ccccactctc tgggagccct caggaatgaa cttgctgaat gtgggaacag 60

atggtcagag tccctcgggg tacctcccat gaaaccctca tctaagaagt cacccaccca 120

c 121

<210>173

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>173

cgaggttcca ccctgaacac cttcctgctc cctcgtggtg tagagtgatg taagccatcc 60

gatgtaggag atgataggcc tggtatggaa tgggggtgcc cgccctccac tcacctgaag 120

t 121

<210>174

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>174

tccgatgtag gagatgatag gcctggtatg gaatgggggt gcccgccctc cactcacctg 60

aagtggccaa ggtggtagaa catgggctgt ttgtaaaacg tgtccttggt gatgtctaca 120

a 121

<210>175

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>175

aatgatggga ctgtcgacaa agttacgcac ccaattgggt cctccttcgg ggttcagggc 60

aaggttccag tcggtccagc cgaccacatg gtacaggagg ttctagggta aggacaaagg 120

c 121

<210>176

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>176

aggttccagt cggtccagcc gaccacatgg tacaggaggt tctagggtaa ggacaaaggc 60

aaagagacaa aggcgcaaca ctgggggtcc ccagagagtg taggtaaggg tcacatgtgg 120

g 121

<210>177

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>177

ctcaaaaggg caggctagct ggggaaagct ggacaggaag ggcttctgtc agtctttggt 60

gaaactagta agaggtctga ggtctgcttt gcaggaaggg agactggggt ggcttaccgt 120

g 121

<210>178

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>178

gtaagaggtc tgaggtctgc tttgcaggaa gggagactgg ggtggcttac cgtgatgatg 60

ctgtggctgt actgcatccc tcgatcccag gagcctagcc gcacactctg ctcccagaac 120

t 121

<210>179

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>179

cttggagccc acacaggcct ctgaggcaaa gagcatggtg ttggggaaca ggcggtgtgt 60

ctcccctagg gtggctttgg ctggagccag aaagtccagg taccaatgta cagcaatgcc 120

a 121

<210>180

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>180

catgaacata tttagctgct tctgggtctg tcagtacctg caaaggaaga gcaactgatc 60

ctggaccttg cacacaggct tctggaactt ctagttcctg ttgtaggaat cctggagttg 120

g 121

<210>181

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>181

ggaatccata gttgggtaga gaaatcgctc taagtttggg agccagtcat ttggatgctg 60

gatttgaagg tcactggagc accatggagg tccaggcctt accacctttg cccagtgggg 120

c 121

<210>182

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>182

tggcgagggt aggacctagg tcacgggcaa tgaagtctcg ctgatgttca ggggtgaagc 60

ccaggcactg gaaggggtat ccactcaaca gcccagcaga aggctcattt tcagctgtca 120

c 121

<210>183

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>183

gcccagaact gtaacttgtg ctcagcatag gcatccagga acctggcaag agaaaggtca 60

tgaatgatcc ggccaagaaa gtggaccaga ccagctgggt gtggtggctc acacctgtaa 120

t 121

<210>184

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>184

ctgaggacag gcagatctgg aagtggaact aggttgaggg ttgggacaca gatcagcatg 60

gctaaatggg aggccagtcc tgatcccaca tccttgctga tcccttactt cacaaagtat 120

c 121

<210>185

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>185

gcccaggtct ggtggtagat gtctccgggc tgtcccttga gtgacccctt cccattcacc 60

gctccattgg tcttgagcca agtgggtgat gtccaggggc tggcaaggag tgaaacggga 120

c 121

<210>186

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>186

gaaacgggac gctgggccaa ctgcagggct cggtgaatca ggggtatcta gagacaaagg 60

tagtgaagag agaagcaccc agagttggaa cacatactag cccaaccagt gcatccggtt 120

c 121

<210>187

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>187

cagcagggga caaaatgtct gtacaagcag acctacccta cagtttctca acccccagac 60

atcagggccc tcagggcctg aaaaagctag aatgcctacc ttgagcttgg tatcttcctc 120

t 121

<210>188

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>188

ctgggaggct gaagttgtgc aactggaaat catcaggggt gtctgcatag gtgtaggtgc 60

ggatggagaa gtcacagctg gccatgggta cccggatgat gttatatccg attcctacag 120

a 121

<210>189

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>189

ggagaagtca cagctggcca tgggtacccg gatgatgtta tatccgattc ctacagaaaa 60

ggatgatcaa gatatggtag tccgagtcaa taggagagta tgggactctg cttatcactt 120

g 121

<210>190

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>190

tgagattctg cctcaaaaaa attttaaaaa aagaaaagaa aaacgaaaag tttcaatggc 60

tctatgtcat cttgtcccct tcctcctcac cttcttcaga gaagtacgat ttaagtagca 120

a 121

<210>191

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>191

aaattttggg cagggggtga cagggcaagg atgttgagag cagcagcatc tgtcatggcc 60

cctccaaatc ccttcacttt ctggaacttc tgttctggct gcagggtcag tagcaggcct 120

g 121

<210>192

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>192

gcccctccaa atcccttcac tttctggaac ttctgttctg gctgcagggt cagtagcagg 60

cctgaggaca tccacaggga ataagggtat cagtacccag cgggaaactc catggtgatc 120

a 121

<210>193

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>193

aagggtatca gtacccagcg ggaaactcca tggtgatcac tgacaccatt tacctctagg 60

aggacccagc ctggcccagg gggtgagggg tgtaatggtt acctgtgccc gtgtgattag 120

c 121

<210>194

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>194

agcctggatg ggccccatac tcagctccat ccgtcgccca ctgcgtgtac tctcatagcg 60

gctgaaggta ccaagggcag gaaaggtcgg ggggtcaaag gagtcacagt atgtggcatt 120

g 121

<210>195

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>195

agacacacac caccgagctg tagccgaagc ttttagggat gcaggggcgg gcacctggga 60

gggagggagt acaagcagag tgaggtctga tgaagacatg gagaatggac acatctgcta 120

g 121

<210>196

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>196

aagtctacca ccagcttact ggaaggctac caaaggacta tgaggcagaa gggaggctct 60

gtgctacctc cccactgcct tgactcactc acctgatgcc cacgacactg cctgaagtag 120

a 121

<210>197

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>197

aatcctgtga ggctgccagc catgatgctt accctactca aaggcttggg acattcctga 60

ggacagaatg aggaatgact gaaaagcaag cccctctcca cccctcaact actctcctgg 120

g 121

<210>198

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>198

ggccctggat tcaaagagag tctgtcattc attaaattcc agtgccagga ttccagaagc 60

actgtgacaa tgctgattgg gagctctctc tcttacctct ctggaaggac ttgaaaactc 120

c 121

<210>199

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>199

gcagggagtt tgtggaggag ttcatctggc cggccatcca gtccagcgca ctgtatgagg 60

accgctacct cctgggcacc tctcttgcca ggccctgcat cgcccgcaaa caagtggaaa 120

t 121

<210>200

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>200

cgcccagcgg gagggggcca agtatgtgtc ccacggcgcc acaggaaagg tgaggcacct 60

gggaagggcc gggcagaggg agatggaggc ggaggggtgt gggaaggaga tgagcacccc 120

t 121

<210>201

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>201

tctgcccagc tctgtctccg ccacgggctg tccttgtcct cacgtcctcc cccagactcc 60

agaaccccca tcctgtggct cctgacagcc ctctgttctg cattgcaggg gaacgatcag 120

g 121

<210>202

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>202

cggtttgagc tcagctgcta ctcactggcc ccccagataa aggtaggatg tggctcctcc 60

ccttagcagg gagcactagc atctgcagca cctgatgggg aggagggcac agggttctgg 120

g 121

<210>203

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>203

cccccatggg cccctcccag gagaggccag ctctgcagct tacaggccag gggaagccca 60

cagctcggcc ctcccggctc tgaccccttg tcctatgtcc aggtcattgc tccctggagg 120

a 121

<210>204

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>204

gaattctaca accggttcaa gggccgcaat gacctgatgg agtacgcaaa ggtatggccg 60

agtctcccca ccacccccaa cctttcccta tagccccctt cccgggcacg tccctgctgg 120

g 121

<210>205

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>205

gggggccaga gtttggggct ctctgaaaaa gcagggcccc tgggacggac ctcacgcgtc 60

cttccagccg ccttacctcc acctgtgctg tctctttcct gcagcaacac gggattccca 120

t 121

<210>206

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>206

tcactcccaa gaacccgtgg agcatggatg agaacctcat gcacatcagg taaatcccac 60

cctccaccca tccttggtcc tcccgggctc attccaaagg acggccacgc gctgccccag 120

g 121

<210>207

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>207

atgggcacaa tggggtgtgt gtgttggggg atggggacat gccatgtccc tccccagctg 60

accctgtctt tcctttcccc tccgcagcta cgaggctgga atcctggaga accccaaggt 120

a 121

<210>208

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>208

cccagctgac cctgtctttc ctttcccctc cgcagctacg aggctggaat cctggagaac 60

cccaaggtaa tcccccaaac cccatctcct cccagctggc cacctttggt ggtagcagct 120

c 121

<210>209

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>209

ggagaggggt gcagatcccc gcgggaggtg ggctgtaggg tgtccaggga ctggtatgtc 60

atctgcccac cactttctgt cttttttcag aaccaagcgc ctccaggtct ctacacgaag 120

a 121

<210>210

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>210

gaagacccag gacccagcca aagcccccaa cacccctgac attctcgaga tcgagttcaa 60

aaaaggtatg tgcccacctg ttgggactcg aagggggttg acttttgggg ccctggctcc 120

t 121

<210>211

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>211

cgtttcggga ggagggagag ggtggggtgg cgggtgcaga ctcctccgct gagccgggcc 60

cagaggccca ctgccctctc ttcccaccct aggggtccct gtgaaggtga ccaacgtcaa 120

g 121

<210>212

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>212

ggatggcacc acccaccaga cctccttgga gctcttcatg tacctgaacg aagtcgcgtg 60

agtgtctgca gccctgtccg gcctcttggg aaccgccgtc tcgggcgagc acgagcctgg 120

a 121

<210>213

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>213

atgtgtggcc taagctgtgc ccaccctggg acagcccagc tgggtgggtg actctgagcc 60

ttgcggtagc gcccgaacct aatggaccag ttcttcccac aggggcaagc atggcgtggg 120

c 121

<210>214

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>214

gggccgtatt gacatcgtgg agaaccgctt cattggaatg aagtcccgag gtgagtctgc 60

tcagcctccc tcagggcctg cctcgggacc cagcaaaccc agagatccct gagaccctgt 120

c 121

<210>215

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>215

tattattatt attttttttt ttgtcatttg ctgacagttt gggtttcatg cgtttctctc 60

ttttttctcc ttttccccct gcctggaaaa atggctaggt atctacgaga ccccagcagg 120

c 121

<210>216

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>216

gcaggcacca tcctttacca tgctcattta gacatcgagg ccttcaccat ggaccgggaa 60

gtgcgcaaaa tcaaacaagg cctgggcttg aaatttgctg agctggtgta taccggtgcg 120

t 121

<210>217

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>217

aaatcaaaca aggcctgggc ttgaaatttg ctgagctggt gtataccggt gcgtaagact 60

ctatggctgc cccctctaac cgcctcacaa gggatcccaa agtactatca ggctctcggg 120

c 121

<210>218

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>218

aaccatgggg cacccttcct gtgccccagg tctccctgtg tcctcgcggt gcaggaggcc 60

tccctagtgg tatcctgttt tcctccctgt aggtttctgg cacagccctg agtgtgaatt 120

t 121

<210>219

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>219

gtccgccact gcatcgccaa gtcccaggag cgagtggaag ggaaagtgca ggtgtccgtc 60

ctcaagggcc aggtgtacat cctcggccgg gagtccccac tgtctctcta caatgaggag 120

c 121

<210>220

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>220

gggccaggtg tacatcctcg gccgggagtc cccactgtct ctctacaatg aggagctggt 60

gaggtaggtg ccccacacct cattctgacc cccacttggg cacagagcct atcttttgac 120

t 121

<210>221

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>221

aaatgccctg gcatctggga ggtggggctg cttctggccc cagcagtcct cccttcaagc 60

agaggccagg gccaggctga gctgacaagc ttctactctc cttgcagcat gaacgtgcag 120

g 121

<210>222

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>222

ttatgagcca actgatgcca ccgggttcat caacatcaat tccctcaggt gagaagctca 60

gggccctgac gggccttcag agcctccagg tgtaaagggt aggctttgag agcccccagg 120

t 121

<210>223

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>223

cttaacaaac agctgcccca gccaccccag ctctgcctga attaattgaa cccagtgtgt 60

gttgttattg ttaatttaca tttttctttg ttttgaatct ggtttacagg ctgaaggaat 120

a 121

<210>224

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>224

aatatcatcg tctccagagc aaggtcactg ccaaatagac ccgtgtacaa tgaggagctg 60

gggcctcctc aatttgcaga tcccccaagt acaggcgcta attgttgtga taatttgtaa 120

t 121

<210>225

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>225

acctctcagg ggtctccccg ccaaaggtgc tccgccgcta aggaacatgg cgaaggtgga 60

gcaggtcctg agcctcgagc cgcagcacga gctcaaattc cgaggtaagc cccagaggcc 120

g 121

<210>226

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>226

agtctctgtc tcattctaca ggtcccttca ccgatgttgt caccaccaac ctaaagcttg 60

gcaacccgac agaccgaaat gtgtgtttta aggtgaagac tacagcacca cgtaggtact 120

g 121

<210>227

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>227

ccaacctaaa gcttggcaac ccgacagacc gaaatgtgtg ttttaaggtg aagactacag 60

caccacgtag gtactgtgtg aggcccaaca gcggaatcat cgatgcaggg gcctcaatta 120

a 121

<210>228

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>228

ctcctaatga aatattttct ttctcagtga tgttacagcc tttcgattat gatcccaatg 60

agaaaagtaa acacaagttt atggttcagt ctatgtttgc tccaactgac acttcagata 120

t 121

<210>229

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>229

ttataatatc ttgattatta aatttaaatt gttttagtgg aaggaggcaa aaccggaaga 60

ccttatggat tcaaaactta gatgtgtgtt tgaattgcca gcagagaatg ataaaccagt 120

a 121

<210>230

<211>150

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>230

cttaggcttt catttgtttt tgaacagcat gatgtagaaa taaataaaat tatatccaca 60

actgcatcaa agacagaaac accaatagtg tctaagtctc tgagttcttc tttggatgac 120

aagaaacacc aatagtgtct aagtctctga 150

<210>231

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>231

agaaacacca atagtgtcta agtctctgag ttcttctttg gatgacaccg aagttaagaa 60

ggttatggaa gaatgtaaga ggctgcaagg tgaagttcag aggctacggg aggagaacaa 120

g 121

<210>232

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>232

gaaatgtgcg tttgctcccg taggaagaag atggactgcg gatgaggaag acagtgcaga 60

gcaacagccc catttcagca ttagccccaa ctgggaagga agaaggcctt agcacccggc 120

t 121

<210>233

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>233

tgcagagcaa cagccccatt tcagcattag ccccaactgg gaaggaagaa ggccttagca 60

cccggctctt ggctctggtg gttttgttct ttatcgttgg tgtaattatt gggaagattg 120

c 121

<210>234

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>234

gtcctcggaa ccaggacctc ggcgtggcct agcgagttat ggcgacgaag gccgtgtgcg 60

tgctgaaggg cgacggccca gtgcagggca tcatcaattt cgagcagaag gcaagggctg 120

g 121

<210>235

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>235

ccaactttgc acttttctta aaggaaagta atggaccagt gaaggtgtgg ggaagcatta 60

aaggactgac tgaaggcctg catggattcc atgttcatga gtttggagat aatacagcag 120

g 121

<210>236

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>236

taattcataa tttagctttt ttttcttctt cttataaata ggctgtacca gtgcaggtcc 60

tcactttaat cctctatcca gaaaacacgg tgggccaaag gatgaagaga ggtaacaaga 120

t 121

<210>237

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>237

atccatctga tgctttttca ttattaggca tgttggagac ttgggcaatg tgactgctga 60

caaagatggt gtggccgatg tgtctattga agattctgtg atctcactct caggagacca 120

t 121

<210>238

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>238

ttcattatta ggcatgttgg agacttgggc aatgtgactg ctgacaaaga tggtgtggcc 60

gatgtgtcta ttgaagattc tgtgatctca ctctcaggag accattgcat cattggccgc 120

a 121

<210>239

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>239

tcttcttaaa attttttaca ggtccatgaa aaagcagatg acttgggcaa aggtggaaat 60

gaagaaagta caaagacagg aaacgctgga agtcgtttgg cttgtggtgt aattgggatc 120

g 121

<210>240

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>240

tggtcctttc caagctcact gttctcacca cacggcccca ctccggtttc ctggaggctc 60

ccccagaagc atttggggga catagtccag gcaggtgcct cctcccaggt ggggcaagca 120

c 121

<210>241

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>241

gtggggcaag cacctggcat gatagcggaa ttttaggtgg ccatgattca ggttgcatct 60

cacctcatgg gaaccagact tgggagcctg atcacaggtg ccaaggaggt catcgtccct 120

g 121

<210>242

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>242

tcatcgtccc tgccagagtc ctgatcccag acctgcaacc tcaggggccc ccctgtggcc 60

aggagcacat ccccaaaatc cagccgcact gaccagatgg ggttgttatt gtcccacacg 120

g 121

<210>243

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>243

tcctcagctc ctggccacca aagaagagct tcacataggc atccgtggca gtgaaccagt 60

ccccccacag gccccatgct tggatgaagg tcacctccag ctgggccagg cccctctgcc 120

g 121

<210>244

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>244

gcccctctgc cgagggcagc agtcctgggt ggtgaccgct gagccatggc acacacactg 60

gcatgggtct cgggggctct tctgccgccc tggtgggcac ggccggctgc agtccctcca 120

g 121

<210>245

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>245

cgagccctgt ccgtcaggta ctgactcagg gccctcctca gtgcctcccg ccgcgggtcc 60

tggctgtcca gcagcacgtg caggggttcc agggtgtagt ccaccaggcc agggctgccg 120

g 121

<210>246

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>246

gccagggctg ccgggcagcg agtttaccca ggctgagtac tgctcgggcc cggcctggat 60

cccgaacagc aggtcgttaa tggaggtgtg atggccgcca accacttccg agtggcgctc 120

c 121

<210>247

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>247

ctcccggtag gtttggtgga aggaggccgt catcttgtgc ttcttcttct tctcctcaca 60

ggccttggct tcggcagaga tgctgccgtg gatgcctatg ttgacctggg cctcgacagt 120

c 121

<210>248

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>248

tcgacagtca ggcagtcctc cacctcgttg tccgtgagcc cttccagggc cagctcgcag 60

gtgcgcaggg cagtgagggc cgatatgcgg ccacccagct ccacagcccg gatgaagtgg 120

g 121

<210>249

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>249

gccgtagttg gagataagcc tgaggtaggc gggctgggtg gaggcgttga agtggtgggg 60

caggtccccg agggccctct tgaagtcagg gtgcagcggg ggagtgtgta ccacatggaa 120

a 121

<210>250

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>250

agccccccac ccctagcccc agctctcacc tgtagaagcg gcactccacc gtgtcagtgc 60

tgaagctgta ctggtcctgg tgggtcttct gggctgcaaa gttggctgcc tgtgagtgtg 120

a 121

<210>251

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>251

gctgcctgtg agtgtgagcc ggccacagac acatgcacat tgctggtgggcttaggagtc 60

acgtccagcc cgaccttcca gtcgttgcgg atgctacgag ccgcatcccg ggccacagct 120

t 121

<210>252

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>252

agcttcagtg gagctgactt tggccctggt tacatggcgc tggcagccag agccctgggc 60

ccgccagttg gtgagcgcca gaggcaggcg ctggagggtg ccctcctgta gggcattttc 120

a 121

<210>253

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>253

agggcatttt cacagagggt gcaggtgccg tcgggccgca ggaacctttg tgtgtccact 60

gggaaggagc ccgagcggcg gaggctggtc acgtccacac cctccccggc cagccatgca 120

c 121

<210>254

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>254

ccatgcacca ggcacgaact tgtggctgcg cttgcactct gagcgtgcgg ctgtgtggca 60

cggggcaggg acgggcaggg gcagcagcag gagaaggatg cccaggagga gcagacgggc 120

t 121

<210>255

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>255

cattatctct acttgcaggc aaaatgaaag accggctagc agaacttctg gacttgtcca 60

agcaatatga ccagcagttc ccagacgggg acgatgagtt tgactcgccc cacgaggaca 120

t 121

<210>256

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>256

actcgcccca cgaggacatc gtgttcgaga cggaccacat cctggagtcc ctgtaccgag 60

acatccggga cattcaggat gaaaaccagc tgctggtggc cgacgtgaag cggctgggaa 120

a 121

<210>257

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>257

ggaaagcaga acgcccgctt cctcacgtcc atgcggcgcc tcagcagcat caagcgcgac 60

accaactcca tcgccaaggc catcaaggcc cggggcgagg tcatccactg caagctgcgc 120

g 121

<210>258

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>258

gggcgaggtc atccactgca agctgcgcgc catgaaggag ctgagcgagg cggctgaggc 60

ccagcacggc ccgcactcgg cagtggcgcg catttcgcgg gcgcagtaca acgcgctcac 120

c 121

<210>259

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>259

cctcaccttc cagcgcgcca tgcacgacta caaccaggcc gagatgaagc agcgcgacaa 60

ctgcaagatc cgcatccagc gccagctgga gatcatgggc aaggaagtct cgggcgacca 120

g 121

<210>260

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>260

cgggcgacca gatcgaggac atgttcgagc agggtaagtg ggacgtgttt tccgagaact 60

tgctggccga cgtgaagggc gcgcgggccg ccctcaacga gatcgagagc cgccaccgcg 120

a 121

<210>261

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>261

caccgcgaac tgctgcgcct ggagagccgc atccgcgacg tacacgagct cttcttgcag 60

atggcggtgc tggtggagaa gcaggccgac accctgaacg tcatcgagct caacgtacaa 120

a 121

<210>262

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>262

aacgtacaaa agacggtcga ctacaccggc caggccaagg cgcaggtgcg gaaggccgtg 60

cagtacgagg agaagaaccc ctgccggacc ctctgctgct tctgctgtcc ctgcctcaag 120

t 121

<210>263

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>263

aaatcaggta ttgaaattaa attgaagaca tagctttact ctcttctttg atcataacta 60

aaataatatt ttagacaaaa gctaaaacaa aaagaggata ttatacagat tgctgcttct 120

t 121

<210>264

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>264

cttttccaaa cacttctcaa tatcatcaag cacctgaacg gcagcctttg gaattcgagt 60

cccaggacca aatacattgg aaacaccaac ttcaaacaga aattcataat cctgttgaaa 120

g 121

<210>265

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>265

gaccaaatac attggaaaca ccaacttcaa acagaaattc ataatcctgt tgaaagaatg 60

tgtttaatta ataagagcca ctatttccat tttcacggaa ataaaaccct atcctttctt 120

c 121

<210>266

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>266

atcaccagga aataatatgt tcctaaatct acaagtttat ctttagaacc acaaataagc 60

tatcattact caagattccc atcacagtac tagaaaaata gagataaaaa atacctgagg 120

t 121

<210>267

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>267

tggtatcacc cctccacaca tgacaagaat atctggccgt ccaagggagt taagttcttt 60

gatgagttca ggaactaggg ttttatgacc agcagcgagg gtgcttatgc ccacagcatg 120

c 121

<210>268

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>268

tccgcatcca cagcctgctg ggccacttca cgaggagtct aaacagtcag aaagtaaaga 60

taaatcattg tttattacta gataatgact ataaaaaccc tggaagacaa tctaggcaat 120

a 121

<210>269

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>269

gctcaatgtc tgtcatcatt ttactacatt ttctaaatgt aagtgactag taaatacttt 60

tgaaattccc cccaacagtt tttagtacct ggaaaagagg gcctatgtcc acatcaaaac 120

c 121

<210>270

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>270

accaagatca gcaaatcctg tagcaataac ttttgctcct ctgtcatggc catcttgtcc 60

catttttgct acaagaagac gaggtctgcg accttcacgt tccatgaatt tatgaaccct 120

g 121

<210>271

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>271

ccatttttgc tacaagaaga cgaggtctgc gaccttcacg ttccatgaat ttatgaaccc 60

tgaaaaacat ttaaaaatat atcagtagtt agatactaat tttccaaagg gaagatataa 120

a 121

<210>272

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>272

aaattttctc agttgtatgt aaggaaatta agctcccagt agattcaagg ggattgtgct 60

aacagaaaag atgaaattct ggcctaagaa accttacata ttacctcttg atagcagatg 120

t 121

<210>273

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>273

atctctttac tttctccaaa ttcctggcga tatgctccac tcaccattcg atcattcgct 60

ttatgttcac caaatacctt tttcagggca tctgtgattt ctcccactgt acatctgaaa 120

c 121

<210>274

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>274

ttcaccaaat acctttttca gggcatctgt gatttctccc actgtacatc tgaaacatga 60

aatggtggtt ccattaactt cattattttg tgataaagat atctgtttac cataatattc 120

a 121

<210>275

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>275

tcttttgggc tcacatggtt tacaggatca accttttatt taagtatact ctgaaaagct 60

aaactggtca acttttagtc tttggaaacc tccaaactta tatatcttca ccttgcccga 120

g 121

<210>276

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>276

gatgcatcca ctgcaagagc caggatattt ccatctccgc tagcagcaca ttcggttagt 60

gcagcaagac aacgttcagc caaagcttga tccctgctgg atttgatcta tggaaaaagt 120

c 121

<210>277

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>277

gcaagacaac gttcagccaa agcttgatcc ctgctggatt tgatctatgg aaaaagtcaa 60

ggaaagggac aatttacatg aagagagaat aaaacagaga tcaagagatt agaccctgat 120

g 121

<210>278

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>278

acacctcatg ctgttgtaag gattaattta agcaggacag tttaaaagct tagccagagc 60

ccagaacaca gaaaatactt aaaaaaaaaa aaaaaagcaa gctattaata ccttcttaag 120

t 121

<210>279

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>279

atctgcctgt ttcgcactga agtattatca attgccagaa cttctacagc gtcttctttt 60

tccaactggt acttatttac tccaacaatt acttcagaac ctggtaattt cccaaagaaa 120

a 121

<210>280

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>280

ctttttccaa ctggtactta tttactccaa caattacttc agaacctggt aatttcccaa 60

agaaaaattt tattcacaaa taattaccta attgtaatgc tctggttatg atgtattttc 120

c 121

<210>281

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>281

aagttccatt tttcattatt tttgttttgt taactataca tgttatcttc aaacagaaat 60

gaatttcttt ttaactactg gatttcatat atgaactttc tcactatctt accagaatct 120

a 121

<210>282

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>282

tattctagct tgtcttcggg cagcacattc ttcaattcga agtttaggta ttccctcagc 60

tacagctttg gccattccac ccatttcttc aatttcatta atgagctaaa aagaaaaaca 120

t 121

<210>283

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>283

ctttggccat tccacccatt tcttcaattt cattaatgag ctaaaaagaa aaacattaac 60

aaaactaaaa gagaatatta aaaatgtgtg taaactatat aaatttgtta tgagtgttat 120

a 121

<210>284

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>284

ataaatttaa aatctataaa tcttgacttg taagttttaa aatctataaa tctttacaaa 60

tctgtaaatt ctgaaaacaa agttgcaaag tggaaaaact tacctttaaa gcagcatcat 120

a 121

<210>285

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>285

catttgtgag acattccatc atgtaagaac ctccccaagg atcagccact ttgggaatcc 60

cagattcttc ttgaatgatg atttgtgtgt tcctggcaat tcgagcactt ttcacagttg 120

g 121

<210>286

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>286

cacagttggc aaacccaaag cttcatcaaa agaatttgtg tgcaaagact gagtccctcc 60

aaatactgct gccattgctt ctattgcagt acggacaata ttattgtagg gatcctaaaa 120

t 121

<210>287

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>287

atactgctgc cattgcttct attgcagtac ggacaatatt attgtaggga tcctaaaata 60

tttgataaaa aacaaaaact caaagaaaca ggtgatagat attgcaacta taaacagcaa 120

c 121

<210>288

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>288

aaatattaac agatagcttc ttaattctat ttcctgcttg tgccacattg ctcagaaaaa 60

atatatatat ataactttat taaaattcta cattttaaat tatatacata cctgctcagt 120

a 121

<210>289

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>289

taagtgacca tccagatgtc tgacagtgtg ctcttagaag aagagatttt gagtttttag 60

gctgaaacat tttctctatt aagtgagccc agagtcttct accagctctc atctttgcta 120

t 121

<210>290

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>290

atatagaaat tcattccaat tccccagaag aaagacaacc taaaatagta acgttaggtc 60

cagaatttaa ttaaagttaa caatatagag cagaaaataa aactaattgt atcacacact 120

a 121

<210>291

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>291

tttataaatt cattttatca atatataaaa tggtcctatg catttcttaa tgttgaaaca 60

atatctaggt ttaatttact caccttggtg caaattcatc aattgtcagg ccagcctgga 120

g 121

<210>292

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>292

ctggagtcca gttctagagt actccaatcc atctgctaaa gtataggcca gctccagaat 60

ggcatcagcc cctgcttcct gcatatggta tccactaatt gaaattgaat taaattttgg 120

c 121

<210>293

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>293

atcagcccct gcttcctgca tatggtatcc actaattgaa attgaattaa attttggcat 60

gtgctacata aaaaaaaaaa ttgtaacagt gaataagtaa aaatattaaa aggtcctatt 120

a 121

<210>294

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>294

ttactcagac taaattttta aattcagtta tagcatgttg taaaaattcc tacattcaag 60

gaactataga aaaacctata ataaccacaa agtatacctt tgctgtatat tcaaatatgt 120

c 121

<210>295

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>295

gtcagcaata attttcatgg atggttctgg aggaaaaatg tatgtatttcgaaccataaa 60

ttcctttagt atatcatttt ggatggtacc agtaagcttc tctttaggta caccttgttc 120

t 121

<210>296

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>296

cttctccagt tactataaaa tttgcaagaa ctggaataac tgctccattc atagtcatgg 60

aaactgacat tttttctaaa ggaattccat caaaaagaat tttggtatct tccacagtgt 120

c 121

<210>297

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>297

tgtcaatagc aactccagcc attccaacat caccacgaac tcgagggttg tctgaatcat 60

agccacgatg tgtcgccaga tcaaaggcaa ctgataatcc ctgctgacca gctaaatata 120

t 121

<210>298

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>298

catagccacg atgtgtcgcc agatcaaagg caactgataa tccctgctga ccagctaaat 60

atataaagaa aaataatgta agattcaaga gtctggaatc aaggtaaaat gatttacttt 120

t 121

<210>299

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>299

tcatctttac agagattaac ccccaaaaaa taaaaaacta ggaggcatat gacttatcat 60

aaatattatg tcttacatta aaatctcacc cttaatgttg tccttataga acttattgct 120

t 121

<210>300

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>300

tgctttcttc cacagtacta aaaccagcat actggcggat ggtccagggc ctaaaggtat 60

acatggtagg atatggtcca cgtgtgaatg gcttcactcc tggaagttct tcaggtaagt 120

c 121

<210>301

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>301

agtatctctc ttggaataca agggttttat agagatccct tccggggtgt gccatattag 60

gtcttctggg tttttgcctt tcagctgctt tttagccagg gcagcccatt ctgggtgaag 120

g 121

<210>302

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>302

tgggtgaagg ggctgttgct ggtgtagaag tcgttgctgt atgagcctgg agcctgatga 60

ttcttttacc tgcctcaggt aatgaggtga aagtaaaaaa agctgattct tagctcttaa 120

c 121

<210>303

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>303

ttacctgcct caggtaatga ggtgaaagta aaaaaagctg attcttagct cttaacatgg 60

tggagcatgg aaacacccaa tagaaataag aactgaccta gaaaaagaaa catagagtaa 120

a 121

<210>304

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>304

gctcccaagg cggccctccg cgcaatgccg ctaccggaag tgcgggtcgc gcttccggcg 60

gcgtcccggg gccagggggg tgcgcctttc tccgcgtcgg ggcggcccgg agcgcggtgg 120

c 121

<210>305

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>305

gcgtcccggg gccagggggg tgcgcctttc tccgcgtcgg ggcggcccgg agcgcggtgg 60

cgcggcgcgg ggtaagtggc ggtccccacg gggcaagtgg cggtccccac ggggcagcgg 120

c 121

<210>306

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>306

cgcggtggcg cggcgcgggg taagtggcgg tccccacggg gcaagtggcg gtccccacgg 60

ggcagcggcc tagagaggcg gaccccgcag tgtccgggtc ccgggccctc acccgcgccc 120

a 121

<210>307

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>307

gggagggtaa gggctcctca gctccctccc tggactccca gttcagtcac cccttccccc 60

ggaagaattc aaagaggaag ggcagggtct atgtcatgga cactgctact tgttcgatga 120

a 121

<210>308

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>308

gccaggttta tgtcccgttc tgaaaggccg gcacactcat gggtgctcag cgtgatgtgg 60

acctgaaatg aaaccagaaa ttctgcttcc actgacttca cttaagaaca ggagggagtc 120

a 121

<210>309

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>309

aggctcattg ttaagtccaa aagccttcag aatgtgtcag agttcgcagt atttggatgg 60

gggcagagca caacagaggc acacagcatc actcaccttg ttgtacacgt taaaccattc 120

a 121

<210>310

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>310

caggatggtg gtccagtttc tcagcctgca gggccactct tgtcatgaac ccaaaggcct 60

atttaagtgg agtgagagcc aggttagtgt tctaagagaa aggacttctg gacatcacca 120

a 121

<210>311

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>311

gagtcttggc taacaagacg tgaagggaga gaacatgctt tgtaaggtga ccccatcagc 60

ccgtctcaga aaactctgtc ccaccctggt gccataccct gttgaagtct ttgaaatgaa 120

a 121

<210>312

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>312

aaactgcttg aagatggcat cacggccttc cagctcattc caccccacag ccctcaggtt 60

tggcagcagc tggtccctct cctcagcgct cagcctgtgt gctttgccag cctagaagag 120

g 121

<210>313

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>313

ttggcagcag ctggtccctc tcctcagcgc tcagcctgtg tgctttgcca gcctagaaga 60

gggaaaaaaa cagaggccca agggcatttc tctttatagg tcaaaacaga ggggctccaa 120

c 121

<210>314

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>314

ccgcagggga ctcgaaaaga ctttcccccg ccccttcccg ctcccacctc gcccgcggct 60

gccccggccc cgctgccccg atcgcggccg cgcacccctg gactcaccat ggcgcgggcg 120

g 121

<210>315

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>315

tggcacgcac tgcgcagccg cggtgggagg aggcaccggc cgcgcggcgg gaggaggtgc 60

cggccgagca ccgcagacag cgccgggaag atgagcacgg aaggtggtgg ccgtcgctgc 120

c 121

<210>316

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>316

caggcacaag tgtcccgccg catctccttc agcgcgagcc accgattgta caggtagggt 60

gtgcacacag gtacagcggc gggcggtggg cgccgggccc cggaacgtca ccggggcggg 120

g 121

<210>317

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>317

caaagatcag tacaaataat aaatataagg aacagagaag ggggtttgaa tgtgatactt 60

gtgtcatgct gacttttttt tttttttttg gtcagtaaat ttctaagtga tgaagaaaac 120

t 121

<210>318

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>318

gaaactgttt gggaaatgca acaatccaaa tggccatggg cacaattata aaggtgagag 60

aaaaactgat gacatttcag cccttcaata aggatgaaag agtattcagc aaatgtagac 120

a 121

<210>319

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>319

ttgccaactt gtgcttgtat gttgctaact tgtgcttgga tgttgatctg ttgaaagtca 60

tgctgttttt tttgtatttt gttttctttc catagttgtg gtgacagtac atggagaggt 120

a 121

<210>320

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>320

gttttttttg tattttgttt tctttccata gttgtggtga cagtacatgg agaggtatgt 60

gcagaaaata tttgtgtggt ttttgcagat tgctgggctc tctttcagcc agtgtggtgg 120

a 121

<210>321

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>321

tgtgcaaatg tgggcacagt ctctgcacat tgtactgcct ttaataattt gccagccgtt 60

taatatggag agcctatcac agtaatattc acctttgttt attctttaga ttgaccctgc 120

t 121

<210>322

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>322

cgggaatggt tatgaatctg gctgatctca aaaaatatat ggaggtaatg gcatgttggg 60

tgcttattat gtgctattcc ctaactgtaa tatttggtgg ccccctatct acctccccaa 120

c 121

<210>323

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>323

gtggctaagt gataaggtga ggtttagagg cataagtgga acaatttgga atttgagtcg 60

taaatggagt caatgatatt ttcccttggt tttgtctcta ggaggcgatt atgcagcccc 120

t 121

<210>324

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>324

ccttgatcat aagaatctgg atatggatgt gccatacttt gcagatgtgg tgaggtgggt 60

ggcactgtat cttgccttat gtggattgta aaacaagaat tgatttgaat actttgattg 120

t 121

<210>325

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>325

aaatctttcg aaactagaat ttctattttc tgtaaatatt aaacatgaaa ttttattgtt 60

tgcattttga attttttttg tttttgtttt tttttcttat agcacgactg aaaatgtagc 120

t 121

<210>326

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>326

tgtttatatc tgggacaacc tccagaaagt tcttcctgta ggagttcttt ataaagtaaa 60

agtatacgaa actgacaata atattgtggt ttataaagga gaatagctat tggggttagc 120

a 121

<210>327

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>327

atcgttggta cgatacgctt tggttaaaac gttggacaca gctcataatg tcttccaccg 60

tcagttcatt ctgtgctcgt tcaggtgcgt ggaagctatg gttctgcaga cctgaaaatg 120

a 121

<210>328

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>328

ccaccgtcag ttcattctgt gctcgttcag gtgcgtggaa gctatggttc tgcagacctg 60

aaaatgatgg gcactctcaa atgtttctgg aaatacttag aaaaatatct tataagatta 120

a 121

<210>329

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>329

gtactatttg aaaaaaatac actaattctt ctcccttccc aggcccctct ggttatctgg 60

cagtggtttt gtgcacgtac ttacactatt agcagttcac tttaatattg ccacaaaaag 120

g 121

<210>330

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>330

tggcaagaag aaagtagagg gctcaaccct ttattatatt tatttgactt cctagaaata 60

attttaaata taattagtga caaggaataa agttcacatc tgtaacaatt gaaaatggaa 120

t 121

<210>331

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>331

acatctgtaa caattgaaaa tggaatgtac aaacaagacc ggacagacag acaatgctac 60

tggcagtacg atcggcaacc aacgcacaca cactgaatga agctcagctc ctaatgagag 120

t 121

<210>332

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>332

aggaactctt cccgagtctt tggatcctcc cggaacacac ccaacattgt gctggtcaca 60

gttttgctgt tcattttctg tacacctcgc attaccatac acatgtgtct acaaaataag 120

g 121

<210>333

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>333

tttgctgttc attttctgta cacctcgcat taccatacac atgtgtctac aaaataaggc 60

aacacaggtt gtttaaagga gtagacagct gctggtttgg tttttaaaag caggcctaaa 120

g 121

<210>334

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>334

acctggtgct acaaaatatg agaagcacta aatagcaaga tcacttctag tgcaccatta 60

tgacgttact aaaggcagat gcagacttac gttgcttcaa ccactacccc gactccagca 120

g 121

<210>335

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>335

ccagcaggcc gcaaggcttc cgtgattgct acagcaattt gttttgtaag gcgctcctga 60

actgtggatg tgataaggag ctcagtttga gagtctgaca caaacagctg gaagcttttt 120

c 121

<210>336

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>336

cagagcaaga ctctgtctca aaaataaaaa tttaaaaaaa gaaaaaaaag aaaaatgttt 60

tctgttaata cagattttta aagcttacct tgtagtcttc tactatagat ttctacaatc 120

c 121

<210>337

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>337

tgttaataca gatttttaaa gcttaccttg tagtcttcta ctatagattt ctacaatcct 60

agaaaagaaa gaattgtttt agttaatcac aaatcatttg aagtaaaatt gataagcctt 120

c 121

<210>338

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>338

gatccataca ggtaaagaga gaaagcctga tgagatagat tctcagcaga tgagggcagg 60

tcctataaac ctgtattctt gttcactgca cagtcacact tacctcgcaa gtttgctgag 120

g 121

<210>339

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>339

gctgaggcca aggacttgct tgttaggaag ataaccaata tggaccttcagagaagagac 60

ggaaatcatt agctgagtga aaatacagtg ccttctttgt gacaaaataa ggaaatatag 120

a 121

<210>340

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>340

acctgagata tcagcaattg gcagctaaaa attttaaata taattattct aattgaaaaa 60

ctttcactat gttttaaatt gctgggaaac aacaaagaga accttacctt tccaacaaat 120

g 121

<210>341

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>341

caacaaatgg aaccaagtga tgctcacaca tggaaaacat gtctatgtcc ttcacaatca 60

ccatctcatc atgatcttca tcaaatatag catcgtttag gacatctgaa atcagaggct 120

t 121

<210>342

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>342

catcatgatc ttcatcaaat atagcatcgt ttaggacatc tgaaatcaga ggcttgcttt 60

agtaacatgt ccaattttat agaaaggtag aattatggat aaacatgaat agacagtcaa 120

c 121

<210>343

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>343

gcccggcttc ctgcgccaaa agtgaggcaa ctccggaaac ttcctggagc cggcgcgcgt 60

ttcctgcaag caccgccccc gccgcccgca cgctctagca gcccgcgggc gcactgacct 120

g 121

<210>344

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>344

gagatggtct cctggtagcc cttggtgaag aactgcatgg ccgaggccgc cctccagggc 60

gtcttgagca gcccttgccg ctgggggttc tcgcccagcg agctcaggat ggacgagtag 120

g 121

<210>345

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>345

ctgccaggtt agggaggttc agctcgttat cctcctcgct gcggggccgc tcgcccttcc 60

agccgtccgc gggctgcgcg ctcttggcct cgggccgcgg gggcttctcc gccggcctgc 120

t 121

<210>346

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>346

ggcccgggcc gcggcggatc ccgctcgggg aacccattgc tgcacctggc gccccgcggc 60

ttctccgccg gtgcccgcac agggcccttc tccatggacc cgccgcagcc gctgccgttc 120

g 121

<210>347

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>347

cttctccgcc ggtgcccgca cagggccctt ctccatggac ccgccgcagc cgctgccgtt 60

cgggaaggac cccggggcgc ttcgaggtct gcggctaaac tccgccggtg gccgcggaca 120

a 121

<210>348

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>348

ctcattatct cgtaccacaa acaggggtta cagaaaacac aaggctggac aaggccacac 60

tgagacaggt tagtgacttt tctggcaggc ccctcatagg cactgagatg aggcctaaaa 120

a 121

<210>349

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>349

atgctggggt gagttccgtc cttccttctg tggttaccac ctggattagg cttcctgagc 60

tcggtcggtt tttccctgtg atccagtcat ggaaagttct ggaacagaaa ataaaagttt 120

t 121

<210>350

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>350

tcggtttttc cctgtgatcc agtcatggaa agttctggaa cagaaaataa aagttttttt 60

tatattctca aagcaaaaat ctgggcacat gctgaccttc acagtgacgg cttgtggggt 120

g 121

<210>351

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>351

tgacgatctc agggaacaca gacttgtcct ctggaccaca gcaggcagag aataggggct 60

ggaagctgct cagtcatgga catgtctgta atactcactc aactaggaat tctaagggtg 120

t 121

<210>352

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>352

tgaagtcagc ctcaggcatt gatttcctgt tcatcggggt atccagggta accctgcaat 60

ggacacagat aagcacgtca ttcatgtcgc tcctttctaa caacaggtgc ccagtccccc 120

t 121

<210>353

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>353

cagaggtgag agcacaccca ggtcgcctgt ggaaagctac agtcagacaa acggtcacct 60

gcagcagtgg ggcagaggtg ggcagcagcc agggaacccc aagcacttac gggagcacag 120

c 121

<210>354

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>354

cacagcgatg gcggctgccc cgggcggcat gccgctgttc ttcccagcca ggctctggca 60

gagctggtga acagcaccct tggccatgcc gtacccgatc atacctggga aatggggaga 120

a 121

<210>355

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>355

ctggtgaaca gcacccttgg ccatgccgta cccgatcata cctgggaaat ggggagaaga 60

tggctcagtg accactggcg gccaggggga cagcaaggac atgcaatctt ctcttgagat 120

c 121

<210>356

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>356

gtaaaggctt taacagtcct catcccatga aagtgcccag cagccccagc aagcaaggta 60

agcaacccca ctccacagat agggaaataa aggcttacca ggagtcccat ccagggcagc 120

c 121

<210>357

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>357

ctttgcgcca gccaaggtca ggaggcctcc ttccttgaga tgcttggtag ccagatggct 60

ggagatggtc gatgtccata tgctctgctt ccacatcagg tcacagttct taaagagagc 120

t 121

<210>358

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>358

ggtcgatgtc catatgctct gcttccacat caggtcacag ttcttaaaga gagctgagtg 60

aaaaaaacat gtgggctcag cattcccagg aaagaaacca aaaggtgagc tcaactcatg 120

c 121

<210>359

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>359

ggcctgtcac ctagtgcaaa cccaatcctt gtcagctgga aaaagagcag agcagaacaa 60

atgagtgact caggaaagga ctcacacttg gatttggcat tgcccccggc ccatcctcca 120

g 121

<210>360

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>360

tcctccagca acgcaaagaa ttgcatccac cttctcttca cccaagagct ttccaacctc 60

agcagtcacc ttcaacacaa gaacaaaaaa atgtataaac tgtaaggtaa gccaacacag 120

g 121

<210>361

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>361

gaagaacata cagccaaagg aagaacatac agccagtggt cacctctccc cagggttccc 60

ctcacaatgt ttgggggcct ttttgaaact acctggtcag cctgctcagt gaacgagtct 120

g 121

<210>362

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>362

ctgtcatttt aacaatgatg ctagcgctgg cctcttcatt ctccaccaca tcaacgctgg 60

caacccactg gaaggagaaa acagctttgg ttaagaggca gtgagttgtt attccatgaa 120

a 121

<210>363

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>363

ggggtgcaca ggggccccca ccttcctcta gactgccccc cgccgggttc cacacgaaag 60

cccccggcca cccccgcgga gacccagcag ccccagcccg cagcattacc cagttgcggg 120

c 121

<210>364

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>364

gggcccgaaa agcctgcacg catcgagaac ccagagcgcc cctgccgccg tacaccagca 60

cccggcgcgc ctcgcctgca gccgccgccg ccgccatcct gctcctgcca gcccggctcc 120

c 121

<210>365

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>365

cagcacccgg cgcgcctcgc ctgcagccgc cgccgccgcc atcctgctcc tgccagcccg 60

gctcccgcag ctccgaatgc ctcgagccgg agcgccgcgc cccgccccgg ccggccaacc 120

c 121

<210>366

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>366

cgcctgccct ggcgacactt tcgaaggact ggagtgggaa tcagagcttc acgggttaaa 60

aagccgatgt cacatcggcc gttcgaaact cctcctcttg cagtgaggtg aagacatttg 120

a 121

<210>367

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>367

taaaaagccg atgtcacatc ggccgttcga aactcctcct cttgcagtga ggtgaagaca 60

tttgaaaatc accccactgc aaactcctcc ccctgctaga aacctcacat tgaaatgctg 120

t 121

<210>368

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>368

aaatcacccc actgcaaact cctccccctg ctagaaacct cacattgaaa tgctgtaaat 60

gacgtgggcc ccgagtgcaa tcgcgggaag ccagggtttc cagctaggac acagcaggtc 120

g 121

<210>369

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>369

gacgtgggcc ccgagtgcaa tcgcgggaag ccagggtttc cagctaggac acagcaggtc 60

gtgatccggg tcgggacact gcctggcaga ggctgcgagc atggggccct ggggctggaa 120

a 121

<210>370

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>370

tgatccgggt cgggacactg cctggcagag gctgcgagca tggggccctg gggctggaaa 60

ttgcgctgga ccgtcgcctt gctcctcgcc gcggcgggga ctgcaggtaa ggcttgctcc 120

a 121

<210>371

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>371

gaaattgcgc tggaccgtcg ccttgctcct cgccgcggcg gggactgcag gtaaggcttg 60

ctccaggcgc cagaataggt tgagagggag cccccggggg gcccttggga atttattttt 120

t 121

<210>372

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>372

gggatgacac tggtgggaag gaaatgccat cttcaagtgg cttgtggaga acacatatag 60

actattgttt gaggctaatt agaaaaacga gcttgtttgt tgcttttcat tcagtccctg 120

c 121

<210>373

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>373

ttaattcctt tgccattaat ttcttactca tagtaatagt atctctgtga ctgactaaat 60

cccagttgtt tcgatatatc actagaatgg ccacataaac ctgggtcctt attaatatac 120

g 121

<210>374

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>374

tatacgttgc ataatacctt ggatgatctt taccaaacgc acttgcttta cagatatcag 60

atctttcctt gaaaacaaca ggattggaat tgtaactaac acttccgtgc tgagagtgtc 120

t 121

<210>375

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>375

cccggattca ccataatcat aatctgcact accatagctg ccatgtctcc aaactaaacc 60

tgattgacct ttttgccttt cagtgccctc agattgataa tgataagaac tagaactgtg 120

a 121

<210>376

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>376

gtgaggactg ccacgtgact gtattcctga gtgatacgca gaatcttgtg aaagactact 60

aaagtgacca tgttccttag cggtactaga gtctgactgt acaggtgaag actgtacatg 120

a 121

<210>377

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>377

ctggctgtat cgcggtgaga ggatccgggg tgtctggagc catctcttga ctgctcccga 60

gaagatccat gatggtttct ggaagcagac tcagatcgcc tctcagagtc ctctgggtat 120

g 121

<210>378

<211>121

<212>DNA

<213>Artificial Sequence

<220>

<223> Artificial sequence

<400>378

ctgggtatgc ctcactgtca ctgtcctggc taacactgga tccctggcgc ctgcttctcc 60

tggaccccgc tgattcaccc tggccggact gtgagtgtct agagctgtca gcctgagtgg 120

a 121

Claims (10)

1. A probe set which recognizes the causative genes of: thalassemia, phenylketonuria, hepatolenticular degeneration, tuberous sclerosis, tetrahydrobiopterin deficiency, hereditary deafness, gaucher's disease, amyotrophic lateral sclerosis, methylmalonic acidemia, Usher syndrome, albinism, pulmonary cystic fibrosis, citrullinemia type I, X-linked congenital adrenal insufficiency, Fabry's disease, ichthyosis, familial hypercholesterolemia, hemophilia, osteogenesis imperfectasis and familial hemophilocytic lymphocytosis.

2. The probe set of claim 1, wherein the probe recognizes a protein coding region or a splice site region of a causative gene of the disease.

3. The probe combination of claim 1, wherein the pathogenic gene is one or more of the following genes:

ASS1, ATP7B, GBA, GCH1, G L a, HBB, KRT1, KRT10, F L G, STS, MUT, MMAA, MMAB, MMACHC, NR0B1, PAH, PCBD1, PRF1, PTS, QDPR, SOD1, STX11, UNC13D, TSC1, TSC2, USH1G, F8, F9, TYR, S L C45A2, OCA2, CFTR, USH2A, CDH23, PCDH15, USH1C, USH1G, MYO7A, CO L1 a1, CO L1 a2 or VAPB.

4. The probe combination of claim 1, wherein the probe combination comprises a mixture of probes having sequences shown as SEQ ID NO1-SEQ ID NO 378.

5. A gene detection kit comprising the probe set according to any one of claims 1 to 4.

6. The gene assaying kit according to claim 5, further comprising a negative control and a positive control for a pathogenic gene.

7. Use of the probe combination according to claim 1 for the preparation of a diagnostic reagent for a disease according to claim 1.

8. The use of claim 7, wherein the combination of probes is used to identify or bind to the disease-causing gene of claim 1.

9. The use of claim 7, wherein the combination of probes is used for screening or detecting human-related monogenic disorders.

10. The use according to claim 7, wherein the combination of probes is used to detect the genotype of the protein coding region or splice site region according to claim 2;

the detection comprises the following steps:

(1) obtaining separated and purified genome DNA;

(2) adding a probe combination according to claim 1;

(3) analyzing and determining the variation of the target gene in the sample.