CN111454921B - Ketoreductase mutant with improved enzyme activity and application thereof - Google Patents

Abstract

The invention discloses a ketoreductase mutant with improved enzyme activity and application thereof, belonging to the technical field of biology, wherein the ketoreductase mutant is a wild ketoreductase derived from Meyerozyma guilliermondii and can convert 4-chloroacetoacetic acid ethyl ester into (R) -4-chloro-3-hydroxy ethyl butyrate, and compared with a wild sequence, the ketoreductase mutant has higher alcohol dehydrogenase activity and more than 90% of similarity with SEQ ID NO. 8. The ketoreductase mutant of the invention has obvious high specific enzyme activity, is improved by 2-10 times compared with wild ketoreductase, has mild reaction condition, low requirement on equipment, no need of high temperature or cooling in the production process and low energy consumption, and has high efficiency, specific selectivity and convenient purification due to enzyme catalysis, in addition, most of solvents are water, no solvents such as butyl acetate and the like are added to form a two-phase reaction system, the discharge of three wastes is low, and the preparation process is green and environment-friendly.

Description

Ketoreductase mutant with improved enzyme activity and application thereof

Technical Field

The invention relates to a ketoreductase mutant with improved enzyme activity and application thereof, belonging to the technical field of biology.

Background

Ketoreductases are versatile catalysts that selectively reduce an aldehyde or ketone enantiomer to the corresponding alcohol. The (R) -specific ketoreductase enzymes have different properties from the (S) -specific ketoreductase enzymes, and these catalysts are used more and more frequently in the industrial synthesis of optically active alcohols. Optical activity is a prerequisite for the selective action of many pharmaceutically and pesticidally active compounds, in some cases one enantiomer having beneficial pharmaceutical activity and the other enantiomer having genotoxic effect. Therefore, in the synthesis of active compounds for pharmaceutical and agricultural chemicals, it is necessary to synthesize optically active alcohols using a catalyst having the required stereospecificity.

Chirally pure ethyl (R) -4-chloro-3-hydroxybutyrate is an important chiral alcohol. (R) -CHBE (ethyl 4-chloro-3-hydroxybutyrate) has been used as the primary precursor for (R) -carnitine, R-4-amino-3-hydroxybutyrate, (R) -4-hydroxy-2-pyrrolidone and other pentaene chemicals. In recent years, a new synthetic route for applying (R) -4-chloro-3-hydroxy ethyl butyrate to super statins is reported, and the patent monopoly of the original synthetic process is expected to be broken. Some oxidoreductases belonging to the SDRs have been screened and used for the biological reduction of ethyl 4-chloroacetoacetate to CHBE (ethyl 4-chloro-3-hydroxybutyrate). Most of the reported microbial enzymes produce CHBE in (S) form, and have both high enantioselectivity and high yield. However, there are few enzymes that produce (R) -type CHBEs, and they are associated with low stereoselectivity (e.g., 70% e.e.) or low yield. Many people report a ketogenic bacillus reductase, wherein a carbonyl reductase can convert COBE into (R) -CHBE (99.6% e.e.) with a yield of 91.7%, but a water-toluene two-phase system is needed, a substrate needs to be added into a reaction system for three times, the production cost is increased, and the production burden is increased; the activity of the catalytic system is low, the reaction can be finished only by inputting a large amount of enzyme or cells (2.158 g of substrate is catalyzed by 0.5g of stem cells), and the process has little amplification prospect for the intermediate CHBE with large market demand and low price.

The ketoreductase from Sporobolomyces salmonicolor is used as (R) -4-chloro-3-hydroxy ethyl butyrate by Kataoka and the like of Japanese, the concentration of a substrate is as high as 300gL, but the chiral purity is only 92%, and the ketoreductase cannot be applied to the synthesis of drugs with high requirements on the chiral purity.

Chinese patent CN104988085A utilizes a biosynthesis method of (R) ethyl 4-chloro-3-hydroxybutyrate and derivatives thereof, takes ethyl 4-Chloroacetoacetate (COBE) and derivatives thereof as starting materials, utilizes recombinant e.

In the Chinese patent CN 103160547, ethyl (R) -4-chloro-3-hydroxybutyrate is prepared by asymmetrically reducing ethyl 4-chloroacetoacetate by using alcohol dehydrogenase derived from Candida albicans, and ethyl (R) -4-chloro-3-hydroxybutyrate is prepared by asymmetrically reducing the ethyl 4-chloroacetoacetate serving as a substrate and NADH serving as a cofactor by using the alcohol dehydrogenase as a catalyst. However, the concentration of the substrate is only 25g/L-50g/L, the enzyme dosage is too high (5g of bacteria/25 ml system), the conversion rate is low, and the industrial production prospect is not provided.

Disclosure of Invention

The invention mainly aims to provide a ketoreductase mutant with improved enzyme activity and application thereof.

The purpose of the invention can be achieved by adopting the following technical scheme:

a ketoreductase mutant with improved enzymatic activity, derived from the wild-type ketoreductase enzyme of Meyerozyma guilliermondii, capable of converting ethyl 4-chloroacetoacetate into ethyl (R) -4-chloro-3-hydroxybutyrate, having a higher alcohol dehydrogenase activity compared to the wild-type sequence, more than 90% similarity to SEQ ID No.8 and having one or more mutations in the following characteristics: R13K, G77D, N89G, T92A, L97N, F131Y, T138P, L146I, G149N, L150V, M154V, V157A, L166Y, V169I, H170K, L176V, A180S, N200A, Y201F, C203A, C208V, C247V, G248S, L278F, S283L, R285L, G295W, W307L, T309S, Q327E, K330E, R331K, and the sequence of the ketoreductase mutant is SEQ ID NO. 6.

A ketoreductase mutant having an improved enzymatic activity, which ketoreductase mutant has an enzymatic activity at least 2-10 times greater than that of the wild-type ketoreductase.

A polynucleotide encoding a polypeptide which is recombinant by a ketoreductase having the sequence SEQ ID No. 6.

A polynucleotide, the sequence of which is SEQ ID NO. 5.

A recombinant plasmid comprising an expression vector having a polynucleotide having the sequence of SEQ ID No.5 attached thereto.

A host cell comprising said recombinant plasmid.

A host cell, which is an Escherichia coli.

A host cell, wherein the codons of the recombinant plasmid have been optimized for expression in the host cell.

A method for producing ethyl (R) -4-chloro-3-hydroxybutyrate, comprising converting ethyl 4-chloroacetoacetate to ethyl (R) -4-chloro-3-hydroxybutyrate in the presence of a ketoreductase mutant having the sequence of SEQ ID No. 6.

The invention has the beneficial technical effects that:

the patent provides a preparation method of a medical intermediate (R) -4-chloro-3-hydroxy ethyl butyrate, the whole system is catalyzed by single enzyme or double enzymes, glucose or alcohol is used for coenzyme circulation, the concentration of a substrate is up to 120g/L-200g/L, the chiral purity is more than 99%, and the dosage of the substrate/NAD is up to 3017: 1, the coenzyme has high cycle times, so that the production cost of the (R) -4-chloro-3-hydroxybutyric acid ethyl ester is close to that of the (S) -4-chloro-3-hydroxybutyric acid ethyl ester, and the downstream application range is effectively expanded.

The present inventors have found that the wild-type ketoreductase enzyme naturally present in the microorganism Meyerozyma guilliermondii has the potential to convert ethyl 4-chloroacetoacetate to ethyl (R) -4-chloro-3-hydroxybutyrate. The inventors of the present disclosure have found that ketoreductases comprising a mutation at a position exhibit increased catalytic activity as compared to the wild-type ketoreductase enzyme produced by Meyerozymeaguilliermondii (SEQ ID NO: 8), "wild-type ketoreductase", "wild-type KRED enzyme", and "wild-type KRED ketoreductase" refer to a ketoreductase enzyme produced by Meyerozymea guilliermondii having the sequence of SEQ ID NO: 8 amino acid sequence ketoreductase. The enzyme can convert 4-chloroacetoacetic acid ethyl ester into (R) -4-chloro-3-hydroxy butyric acid ethyl ester. "wild-type" refers to the same form of material or substance as found in nature. Examples for example wild-type protein or nucleic acid sequences are the original sequence forms which can be isolated from nature and which are present in the organism without artificial modification. "increased catalytic activity" refers to a ketoreductase enzyme that exhibits an increased rate of conversion of a substrate (e.g., ethyl 4-chloroacetoacetate) to a product (e.g., ethyl (R) -4-chloro-3-hydroxybutyrate) as compared to the wild-type ketoreductase enzyme, as measured in an in vitro or in vivo assay.

The present invention provides a ketoreductase mutant, derived from the wild-type ketoreductase enzyme of Meyerozyma guilliermondii, that exhibits an increased rate of conversion of a substrate (e.g., ethyl 4-chloroacetoacetate) to a product (e.g., ethyl (R) -4-chloro-3-hydroxybutyrate). The ketoreductase mutant shows stronger catalytic activity compared with the wild-type ketoreductase of SEQ ID NO. 8. Ketoreductase mutants and polynucleotides encoding such mutants can be prepared using methods commonly used by those skilled in the art. Mutants can be obtained by in vitro recombination, polynucleotide mutagenesis, DNA shuffling, error-prone PCR and directed evolution methods etc. encoding the enzyme.

The ketoreductase mutant described above, having one or more mutations selected from the following features:

R13K，G77D，N89G，T92A，L97N，F131Y，T138P，L146I，G149N，L150V，M154V，V157A，L166Y，V169I，H170K，L176V，A180S，N200A，Y201F，C203A，C208V，C247V，G248S，L278F，S283L，R285L，G295W，W307L，T309S，Q327E，K330E，R331K。

the ketoreductase mutant is preferably selected from the sequence SEQ ID NO. 4. Full-length mutant ketoreductases are not necessary to maintain the catalytic activity of the enzyme. Accordingly, truncated analogs and catalytically active fragments of ketoreductase mutants are contemplated. For example, in some embodiments, several amino acids may be deleted from the C-or N-terminus. Any particular truncated analog or fragment can be used in a corresponding assay to assess catalytic activity. Likewise, additional amino acid residues may be added to one or both termini without affecting catalytic activity. The additional sequences may be functional or non-functional. For example, the additional amino acid sequence may be used to aid in purification, as a marker, or to perform some other function. Thus, the ketoreductase mutants of the present disclosure may be in the form of fusion proteins, in which the ketoreductase mutants (or fragments thereof) are fused to other proteins, such as by way of example and not limitation, a solubilizing tag (e.g., SUMO protein), a purification tag (e.g., a metal-binding His tag), and a bacterial localization signal (e.g., a secretion signal).

The present invention provides a ketoreductase mutant which has at least 2-10 fold increased ketoreductase activity over wild-type ketoreductase.

The ketoreductase mutant coding sequence described above, which is preferably selected from SEQ ID NO.3, has been sequence optimized for expression in E.coli. In some embodiments, the polynucleotide comprises codons optimized for expression in a particular type of host cell. The codon usage and preferences for each different type of microorganism are known as are optimized codons for the expression of a particular amino acid in these microorganisms. The present invention provides a recombinant plasmid, and in some embodiments, the control sequence includes a promoter, a leader sequence, a polyadenylation sequence, a propeptide sequence, a signal peptide sequence, a transcription terminator, and the like. For bacterial host cells, suitable promoters for directing transcription of the coding sequence include, but are not limited to, the genes selected from bacteriophage T5, bacteriophage T7, bacteriophage lambda, E.coli lacUV5 operon, E.coli trp operon, E.coli tac operon, and the like.

Drawings

FIG. 1 is an expression plasmid map of Mgu-3.

FIG. 2 shows the TLC pattern of Mgu-CK, Mgu-1, Mgu-2, Mgu-3, biotransformation reaction for 21 hours, from left to right, Mgu-CK, Mgu-1, Mgu-2, Mgu-3, the upper yellow band as substrate and the lower white band as product.

FIG. 3 is a TLC pattern of Mgu-2 bioconversion for 20 hours under different reaction conditions, and the reaction results of example 12, example 13, example 14 and example 15 are from left to right.

FIG. 4 is a TLC pattern of Mgu-3 at 200g/L substrate concentration for 5.5 hours of biotransformation, with the results of the reactions of example 16, example 17, and example 18 proceeding from left to right.

FIG. 5 is a chiral detection spectrum, which comprises, from top to bottom, an S-type standard, an R-type standard, a product of comparative reaction example 19, and a product of example 10.

Detailed Description

In order to make the technical solutions of the present invention more clear and definite for those skilled in the art, the present invention is further described in detail below with reference to the examples and the accompanying drawings, but the embodiments of the present invention are not limited thereto.

As shown in FIGS. 1 to 5, the ketoreductase mutants having improved enzymatic activities provided in the present example were obtained by the following examples.

Example 1:

the secondary structure and codon preference of the gene are adjusted by a whole-gene synthesis method so as to realize high expression in escherichia coli.

The splicing primers are obtained by utilizing Primer Premier (http:// Primer3.ut. ee /) and OPTIMIZER (http:// genes. urv. es/OPTIMIZER /) to carry out design, and ensuring that the Tm difference is controlled within 3 ℃ and the Primer length is controlled within 60 base.

The above primers were synthesized, and the obtained primers were dissolved in double distilled water and added to the following reaction system so that the final concentration of each primer was 30nM and the final concentration of the head and tail primers was 0.6. mu.M.

2mM dNTP mix(2mM each dNTP)	5μl
		10×Pfu buffer	5μl
Pfu DNA polymerase(10U/μl)	0.5μl
		ddH2O	The total volume of the reaction system was adjusted to 50. mu.l

The prepared PCR reaction system is placed in a Bori XP cycler gene amplification instrument and amplified according to the following procedures: 30s at 98 ℃, 45s at 55 ℃, 120s at 72 ℃ and 35 x. The DNA fragment obtained by PCR was purified by gel cutting and cloned into the NdeI/XhoI site of pET30a by the homologous recombination method. Single clones were picked for sequencing. The DNA sequence successfully sequenced is SEQ ID NO.1 and is named as Mgu-1, and the corresponding amino acid sequence is SEQ ID NO. 2.

Example 2:

the secondary structure and codon preference of the gene are adjusted by a whole-gene synthesis method so as to realize high expression in escherichia coli.

The splicing primers are obtained by utilizing Primer Premier (http:// Primer3.ut. ee /) and OPTIMIZER (http:// genes. urv. es/OPTIMIZER /) to carry out design, and ensuring that the Tm difference is controlled within 3 ℃ and the Primer length is controlled within 60 base.

The above primers were synthesized, and the obtained primers were dissolved in double distilled water and added to the following reaction system so that the final concentration of each primer was 30nM and the final concentration of the head-to-tail primer was 0.6. mu.M.

2mM dNTP mix(2mM each dNTP)	5μl
		10×Pfu buffer	5μl
Pfu DNA polymerase(10U/μl)	0.5μl
		ddH2O	The total volume of the reaction system was adjusted to 50. mu.l

The prepared PCR reaction system is placed in a Bori XP cycler gene amplification instrument and amplified according to the following procedures: 30s at 98 ℃, 45s at 55 ℃, 120s at 72 ℃ and 35 x. The DNA fragment obtained by PCR was purified by gel cutting and cloned into the NdeI/XhoI site of pET30a by homologous recombination. Single clones were picked for sequencing. The DNA sequence successfully sequenced is SEQ ID NO.3 and is named as Mgu-2, and the corresponding amino acid sequence is SEQ ID NO. 4.

Example 3:

the secondary structure and codon preference of the gene are adjusted by a whole-gene synthesis method,

to achieve high expression in E.coli.

The splicing primers are obtained by utilizing Primer Premier (http:// Primer3.ut. ee /) and OPTIMIZER (http:// genes. urv. es/OPTIMIZER /) to carry out design, and ensuring that the Tm difference is controlled within 3 ℃ and the Primer length is controlled within 60 base.

The above primers were synthesized, and the obtained primers were dissolved in double distilled water and added to the following reaction system so that the final concentration of each primer was 30nM and the final concentration of the head and tail primers was 0.6. mu.M.

2mM dNTP mix(2mM each dNTP)	5μl
		10×Pfu buffer	5μl
Pfu DNA polymerase(10U/μl)	0.5μl
		ddH2O	The total volume of the reaction system was adjusted to 50. mu.l

The prepared PCR reaction system is placed in a Bori XP cycler gene amplification instrument and amplified according to the following procedures: 30s at 98 ℃, 45s at 55 ℃, 120s at 72 ℃ and 35 x. The DNA fragment obtained by PCR was purified by gel cutting and cloned into the NdeI/XhoI site of pET30a by the homologous recombination method. Single clones were picked for sequencing. The DNA sequence successfully sequenced is SEQ ID NO.5 and is named as Mgu-3, and the corresponding amino acid sequence is SEQ ID NO. 6.

Example 4:

synthesis of reference protein Ssa-CK Gene sequence:

according to the gene sequence which is shown in U26463.1 and derived from Sporidiobolus salmonicolor, the Shanghai Jieli organism is entrusted to carry out whole gene synthesis on the coding sequence of the protein, and the coding sequence is cloned into pET30a, so that a control protein expression plasmid Ssa-CK is obtained, and the corresponding amino acid sequence is SEQ ID NO. 7.

Example 5:

synthesis of reference protein Mgu-CK Gene sequence:

according to the sequence shown in EDK37381.2, a Shanghai Czeri organism is entrusted to carry out whole gene synthesis on the coding sequence of the protein, and the coding sequence is cloned into pET30a, so that a control protein expression plasmid Mgu-CK is obtained, and the corresponding amino acid sequence is SEQ ID NO. 8.

Example 6:

shake flask expression test:

coli single colonies containing the expression vector were picked and inoculated into 10ml of autoclaved medium: 10g/L tryptone, 5g/L yeast extract, 3.55g/L disodium hydrogen phosphate, 3.4g/L potassium dihydrogen phosphate, 2.68g/L ammonium chloride, 0.71g/L sodium sulfate, 0.493g/L magnesium sulfate heptahydrate, 0.027g/L ferric chloride hexahydrate, 5g/L glycerol, 0.8g/L glucose, and kanamycin to 50 mg/L. The culture was carried out at 30 ℃ and 250rpm overnight. The following day, a 1L Erlenmeyer flask was taken and inoculated into 100ml of autoclaved medium according to the inoculation ratio example of 1: 100: 10g/L tryptone, 5g/L yeast extract, 3.55g/L disodium hydrogen phosphate, 3.4g/L potassium dihydrogen phosphate, 2.68g/L ammonium chloride, 0.71g/L sodium sulfate, 0.493g/L magnesium sulfate heptahydrate, 0.027g/L ferric chloride hexahydrate, 5g/L glycerol, 0.3g/L glucose, and kanamycin to 50 mg/L. The cells were cultured at 30 ℃ until the OD 5-6 of the cells became zero, and the cells were immediately placed in a flask in a shaker at 25 ℃ and cultured at 250rpm for 1 hour. IPTG was added to a final concentration of 0.1mM and incubation was continued at 25 ℃ for 16 hours at 250 rpm. After completion of the culture, the culture was centrifuged at 12000g at 4 ℃ for 20 minutes to collect wet cells. Then the bacterial pellet is washed twice with distilled water, and the bacterial is collected and preserved at-70 ℃. Meanwhile, 2g of the thalli are added into 6ml of pure water for ultrasonic disruption, SDS-PAGE detection is carried out, and the crude enzyme liquid is stored at the temperature of minus 20 ℃.

Example 7:

fed-batch fermentation:

the fed-batch fermentation was carried out in a computer-controlled bioreactor (Shanghai Seisaku) with a reactor capacity of 15L and a working volume of 8L, using 24g/L yeast extract, 12g/L peptone, 0.4% glucose, 2.31g/L catalase phosphate and 12.54g/L dipotassium hydrogen phosphate, pH 7.0. 200ml of culture was prepared for the primary inoculum and inoculated at OD 2.0. Throughout the fermentation, the temperature was maintained at 37 ℃, the dissolved oxygen concentration during fermentation was automatically controlled at 30% by the agitation rate (rpm) and aeration supply cascade, while the pH of the medium was maintained at 7.0 by 50% (v/v) orthophosphoric acid and 30% (v/v) aqueous ammonia. During the fermentation, when a large amount of dissolved oxygen rises, feeding is started. The feed solution contained 9% w/v peptone, 9% w/v yeast extract, 14% w/v glycerol. When OD600 was about 35.0 (wet weight about 60g/L), induction was carried out with 0.2mM IPTG for 16 hours. Taking 2g of thallus, adding 6ml of pure water, carrying out ultrasonic disruption, carrying out SDS-PAGE detection, and storing the crude enzyme liquid at-20 ℃.

Example 8:

and (3) carrying out biotransformation reaction:

680ml of buffer solution (containing 0.1M PB 7.0 buffer solution, 10% glycerol and 1mM zinc chloride), 120ml of isopropanol, 50ml of Mgu-CK crude enzyme solution and NAD with the final concentration of 1mM are sequentially added into a 3L three-neck flask, evenly mixed for 2 minutes, then 120g of ethyl 4-chloroacetoacetate is added, evenly mixed and then the mixture is adjusted to react at the temperature of 25 ℃ in a water bath. And sampled and stored for 21 hours.

Example 9:

and (3) carrying out biotransformation reaction:

680ml of buffer (containing 0.1M PB 7.0 buffer, 10% glycerol, 1mM zinc chloride), 120ml of isopropanol, 50ml of Mgu-1 enzyme and NAD with the final concentration of 1mM are sequentially added into a 3L three-necked flask, mixed uniformly for 2 minutes, and then 120g of ethyl 4-chloroacetoacetate is added, mixed uniformly and then the mixture is adjusted to a water bath for reaction at 25 ℃. And sampled and stored for 21 hours.

Example 10:

and (3) carrying out biotransformation reaction:

680ml of buffer (containing 0.1M PB 7.0 buffer, 10% glycerol, 1mM zinc chloride), 120ml of isopropanol, 50ml of Mgu-2 enzyme and NAD with the final concentration of 1mM are sequentially added into a 3L three-necked flask, mixed uniformly for 2 minutes, and then 120g of ethyl 4-chloroacetoacetate is added, mixed uniformly and then the mixture is adjusted to a water bath for reaction at 25 ℃. And sampled and stored for 21 hours.

Example 11:

and (3) carrying out biotransformation reaction:

680ml of buffer (containing 0.1M PB 7.0 buffer, 10% glycerol and 1mM zinc chloride), 120ml of isopropanol, 50ml of Mgu-3 enzyme and NAD with the final concentration of 1mM are sequentially added into a 3L three-necked flask, and mixed uniformly for 2 minutes, and then 120g of ethyl 4-chloroacetoacetate is added, mixed uniformly and then the mixture is adjusted to react at 25 ℃ in a water bath. And sampled and stored for 21 hours. The samples of example 8, example 9, example 10 and example 11 were subjected to thin layer chromatography and the results of the reaction are shown in FIG. 2.

Example 12:

and (3) carrying out biotransformation reaction:

680ml of buffer (containing 0.1M PB 7.0 buffer, 10% glycerol, 1mM zinc chloride), 120ml of isopropanol, 100g of Mgu-2 enzyme and NAD with the final concentration of 0.3mM are sequentially added into a 3L three-necked flask, mixed for 2 minutes, 160g of ethyl 4-chloroacetoacetate is added, mixed evenly and then the mixture is adjusted to a water bath for reaction at 25 ℃. And sampled and stored for 20 hours.

Example 13:

and (3) carrying out biotransformation reaction:

680ml of buffer solution (containing 0.1M PB 7.0 buffer solution, 10% glycerol and 1mM zinc chloride), 90ml of isopropanol, 75g of Mgu-2 enzyme and NAD with the final concentration of 0.3mM are sequentially added into a 3L three-neck flask, and are uniformly mixed for 2 minutes, 120g of ethyl 4-chloroacetoacetate is then added, and the mixture is uniformly mixed and is adjusted to react at the temperature of 25 ℃ in a water bath. And sampled and stored for 20 hours.

Example 14:

and (3) carrying out biotransformation reaction:

680ml of buffer solution (containing 0.1M PB 7.0 buffer solution, 10% glycerol and 1mM zinc chloride), 120ml of isopropanol, 100g of Mgu-2 enzyme and NAD with the final concentration of 0.15mM are sequentially added into a 3L three-neck flask, and are uniformly mixed for 2 minutes, 160g of ethyl 4-chloroacetoacetate is then added, and the mixture is uniformly mixed and is adjusted to react at the temperature of 25 ℃ in a water bath. And sampled and stored for 20 hours.

Example 15:

and (3) carrying out biotransformation reaction:

680ml of buffer (containing 0.1M PB 7.0 buffer, 10% glycerol, 1mM zinc chloride), 90ml of isopropanol, 75g of Mgu-2 enzyme and NAD with the final concentration of 0.15mM are sequentially added into a 3L three-necked flask, mixed for 2 minutes, then 120g of 4-chloroacetoacetic acid ethyl ester is added, mixed evenly and then the water bath is adjusted for reaction at 25 ℃. And sampled and stored for 20 hours. The samples of example 12, example 13, example 14 and example 15 were subjected to thin layer chromatography and the results are shown in FIG. 3.

Example 16:

and (3) carrying out biotransformation reaction:

100ml of 1M PB pH7.0 buffer solution, 300ml of pure water, 300g of glucose monohydrate, 200mg of NAD, 1mM of zinc chloride, 50ml of Mgu-3 enzyme solution and 10ml of crude glucose dehydrogenase derived from Bacillus subtilis are sequentially added into a 3L three-neck flask, water is supplemented to 830ml of the total system, premixing is carried out for 2 minutes, 200g of 4-chloroacetoacetic acid ethyl ester is added, the mixture is uniformly mixed, the water bath is adjusted to react at 25 ℃, and the pH is controlled to be about 7.0 by 5M NaOH. And sampled and stored at 5.5 hours.

Example 17:

and (3) carrying out biotransformation reaction:

100ml of 1M PB pH7.0 buffer solution, 300ml of pure water, 300g of glucose monohydrate, 66mg of NAD, 1mM of zinc chloride, 100ml of Mgu-3 enzyme solution and 10ml of crude glucose dehydrogenase derived from Bacillus subtilis are sequentially added into a 3L three-neck flask, water is supplemented to 830ml of the total system, premixing is carried out for 2 minutes, 200g of 4-chloroacetoacetic acid ethyl ester is added, the mixture is uniformly mixed, the water bath is adjusted to react at 25 ℃, and the pH is controlled to be about 7.0 by 5M NaOH. And sampled for storage at 5.5 hours.

Example 18:

and (3) carrying out biotransformation reaction:

100ml of 1M PB pH7.0 buffer solution, 300ml of pure water, 300g of glucose monohydrate, 100mg of NAD, 1mM of zinc chloride, 66ml of Mgu-3 enzyme solution and 10ml of crude glucose dehydrogenase derived from Bacillus subtilis are sequentially added into a 3L three-necked flask, water is supplemented to 830ml of the total system, premixing is carried out for 2 minutes, 200g of 4-ethyl chloroacetoacetate is added, the mixture is uniformly mixed, the water bath is adjusted to react at 25 ℃, and the pH is controlled to be about 7.0 by 5M NaOH. And sampled for storage at 5.5 hours. The samples of example 16, example 17 and example 18 were subjected to thin layer chromatography and the results of the reaction are shown in FIG. 4.

Example 19:

control enzyme Ssa-CK conversion reaction:

as the enzyme activity of Ssa-CK in the reaction of catalyzing 4-chloroacetoacetic acid ethyl ester in a single water phase is very low, the reaction can not effectively catalyze the substrate concentration of 150g/L or 200g/L, the reaction system is adjusted to ensure that the substrate reacts completely as much as possible. 100ml of 1M PB pH7.0 buffer solution, 300ml of pure water, 150g of glucose monohydrate, NAD with a final concentration of 1mM, 1mM zinc chloride, 100ml of Ssa-CK crude enzyme solution, 10ml of Bacillus subtilis-derived glucose dehydrogenase crude enzyme solution, water addition to 830ml of the total system, premixing for 2 minutes, adding 100g of 4-chloroacetoacetic acid ethyl ester, mixing uniformly, adjusting the temperature of a water bath for reaction at 25 ℃, and controlling the pH to be about 7.0 by using 5M NaOH. The chiral purity result showed only 90.27%.

Example 20:

thin-layer chromatography analysis:

a small amount of reaction liquid is extracted by ethyl acetate according to the proportion of 1: 3, 0.5ul of reaction liquid is spotted on a silica gel plate, a developing agent is petroleum ether and ethyl acetate which are mixed evenly according to the proportion of 3: 1, and the potassium permanganate develops color after being dried.

Example 21:

and (3) enzyme activity detection:

taking 6 5ml centrifuge tubes, respectively marking 1-6, respectively adding 3mM NADH solution 0ul, 40ul, 80ul, 100ul, 120ul and 160ul, then supplementing 0.1M phosphate buffer solution with pH of 7.0 to 3ml each tube, mixing uniformly, detecting at 340nm and recording the absorbance value; obtaining a standard curve Y ═ k × X of NADH according to the measured values, wherein Y is the value of absorbance, X is the concentration (mM) of NADH, and R2 of the curve is more than 99.5%; diluting the enzyme solution with pure water by a certain dilution ratio (reference dilution ratio: 600-1000 times), wherein the dilution ratio is suitable for changing the light absorption value per minute by 0.02-0.04; 5ml of the centrifuge tubes were sampled and added to the centrifuge tubes according to the following specific examples, mixed rapidly and poured into cuvettes immediately.

Detection reagent	Dosage of
		Isopropanol (I-propanol)	500ul
2％NAD	100uL
		100mM PBS(pH 7.0)	2.35mL
Diluted enzyme solution	50uL

Detecting the change of the absorbance at 340nm, recording a value every 1min, wherein the change rate per minute is basically the same, the absorbance at 0min is S0, and the absorbance at 3min is S3;

the enzyme activity calculation formula is as follows:

enzyme activity (U/ml) [ (S0-S3) × 3ml × N ]/[ kXtime (t/min) × enzyme addition (ml) ]

Wherein N is the dilution multiple of the enzyme solution.

The detection results are as follows:

sample to be tested	Enzyme activity U/ml
		Mgu-1	26
Mgu-2	160
		Mgu-3	114
Mgu-CK	7.6

Example 22:

and (3) chiral purity determination:

taking the standard products of the S-type product and the R-type product as references, purifying the reaction product, and detecting the chiral purity under the following measuring conditions:

ChiralpakAD-3，4.6x100mm，3um；

Hold 5％for 0.5min；

5-50％in 2.5min；

MeCN；3mL/min；

160bar；

25℃；

APCI(+)；

0.05uL injection；

SIM＝167

the results are shown in the following table, and the original map is shown in FIG. 5.

Reaction of	Chiral purity
		Example 10	99.98％
Example 19Ssa-CK control	90.27％

Therefore, the ketoreductase mutant provided by the invention has the ketoreductase activity which is at least enhanced by 2-10 times compared with the activity of the wild ketoreductase. Compared with wild ketoreductase, the conversion rate of the substrate 4-chloroacetoacetic acid ethyl ester to the product (R) -4-chloro-3-hydroxy butyric acid ethyl ester is increased, the substrate concentration per unit volume is further increased, and the production efficiency is improved during industrial amplification.

The above description is only for the purpose of illustrating the present invention and is not intended to limit the scope of the present invention, and any person skilled in the art can substitute or change the technical solution of the present invention and its conception within the scope of the present invention.

Sequence listing

<110> Nanjing Langen Biotech Ltd

<120> ketoreductase mutant with improved enzyme activity and application thereof

<130> 2019

<160> 8

<170> SIPOSequenceListing 1.0

<210> 1

<211> 1047

<212> DNA

<213> Artificial sequence ()

<400> 1

atgctgccgt ctatccagac cgcttacacc ttcaaacgtg gttctcgtga aatcgttcgt 60

cgtgacgact ggccggttcc gcacccggaa gacaaccagg ttctgctgaa agttgaagct 120

gttggtctgt gcatgtctga cctgcacatc ctgatggctc aggaaaccca cgttccggaa 180

accttcgtta tgggtcacga aatcgctggt tctatcgctg ctgttggtgg taaactggaa 240

tctgacccgc gttacaaagt tggtggtcgt ttcaccgttt gcatcggtct gacctgcggt 300

cgttgcgcta actgccgtaa cggtcacgac aactgctgca ccggtaacgg taaattcccg 360

ggtgcttacg gtctgaaccg tgacggtggt ttccagcagt acctgctggt tccggacctg 420

aacaccctgc tgccgctgcc ggacggtctg tcttacgaaa tggctgctgt ttcttctgac 480

gctgttctga ccccgctgca cgctgttcac aaagttaaac cggacctggt tccgaccgct 540

aaaatcctgg ttatgggtct gggtggtctg ggttctaacg ctgttcagat catcaaaaac 600

tacggttgcc acgttgttgc tgttgacgtt aaaccggaac tggaagaatt cgctcgtcag 660

tgcggtgctg acgaattcta caccgacatc aactcttctc cgcacaaacc ggaatctttc 720

gacgtttgct tcgacttctg cggtttccag gaaaccttcg acgtttgcca gaaatacgct 780

cagtctggtg gtaaaatcgt tgttgttggt ctgggtcgtt ctaaactgat gctgcgtaac 840

tacgactctg ctctgcgttc tctgcaggtt atcttctctt tctggggtac cgcttcttct 900

caggaacagt ctctgcagtg ggttaccaaa ggtctggtta aaccgatggt taccaacggt 960

gacttcgctg aactgccgca gtacctgaaa cgtctggcta aaggtgaagt taaaggtcgt 1020

gttgttttcc gtccgtctaa actgtaa 1047

<210> 2

<211> 348

<212> PRT

<213> Artificial sequence ()

<400> 2

Met Leu Pro Ser Ile Gln Thr Ala Tyr Thr Phe Lys Arg Gly Ser Arg

1 5 10 15

Glu Ile Val Arg Arg Asp Asp Trp Pro Val Pro His Pro Glu Asp Asn

20 25 30

Gln Val Leu Leu Lys Val Glu Ala Val Gly Leu Cys Met Ser Asp Leu

35 40 45

His Ile Leu Met Ala Gln Glu Thr His Val Pro Glu Thr Phe Val Met

50 55 60

Gly His Glu Ile Ala Gly Ser Ile Ala Ala Val Gly Gly Lys Leu Glu

65 70 75 80

Ser Asp Pro Arg Tyr Lys Val Gly Gly Arg Phe Thr Val Cys Ile Gly

85 90 95

Leu Thr Cys Gly Arg Cys Ala Asn Cys Arg Asn Gly His Asp Asn Cys

100 105 110

Cys Thr Gly Asn Gly Lys Phe Pro Gly Ala Tyr Gly Leu Asn Arg Asp

115 120 125

Gly Gly Phe Gln Gln Tyr Leu Leu Val Pro Asp Leu Asn Thr Leu Leu

130 135 140

Pro Leu Pro Asp Gly Leu Ser Tyr Glu Met Ala Ala Val Ser Ser Asp

145 150 155 160

Ala Val Leu Thr Pro Leu His Ala Val His Lys Val Lys Pro Asp Leu

165 170 175

Val Pro Thr Ala Lys Ile Leu Val Met Gly Leu Gly Gly Leu Gly Ser

180 185 190

Asn Ala Val Gln Ile Ile Lys Asn Tyr Gly Cys His Val Val Ala Val

195 200 205

Asp Val Lys Pro Glu Leu Glu Glu Phe Ala Arg Gln Cys Gly Ala Asp

210 215 220

Glu Phe Tyr Thr Asp Ile Asn Ser Ser Pro His Lys Pro Glu Ser Phe

225 230 235 240

Asp Val Cys Phe Asp Phe Cys Gly Phe Gln Glu Thr Phe Asp Val Cys

245 250 255

Gln Lys Tyr Ala Gln Ser Gly Gly Lys Ile Val Val Val Gly Leu Gly

260 265 270

Arg Ser Lys Leu Met Leu Arg Asn Tyr Asp Ser Ala Leu Arg Ser Leu

275 280 285

Gln Val Ile Phe Ser Phe Trp Gly Thr Ala Ser Ser Gln Glu Gln Ser

290 295 300

Leu Gln Trp Val Thr Lys Gly Leu Val Lys Pro Met Val Thr Asn Gly

305 310 315 320

Asp Phe Ala Glu Leu Pro Gln Tyr Leu Lys Arg Leu Ala Lys Gly Glu

325 330 335

Val Lys Gly Arg Val Val Phe Arg Pro Ser Lys Leu

340 345

<210> 3

<211> 1047

<212> DNA

<213> Artificial sequence ()

<400> 3

atgctgccgt ctatccagac cgcttacacc ttcaaaaaag gttctcgtga aatcgttcgt 60

cgtgacgact ggccggttcc gcacccggaa gacaaccagg ttctgctgaa agttgaagct 120

gttggtctgt gcatgtctga cctgcacatc ctgatggctc aggaaaccca cgttccggaa 180

accttcgtta tgggtcacga aatcgctggt tctatcgctg ctgttggtgg taaactggaa 240

tctgacccgc gttacaaagt tggtaaccgt ttcaccgttt gcatcggtct gacctgcggt 300

cgttgcgcta actgccgtaa cggtcacgac aactgctgca ccggtaacgg taaattcccg 360

ggtgcttacg gtctgaaccg tgacggtggt ttccagcagt acctgctggt taccgacctg 420

aacaccctgc tgccgatccc ggacggtctg tcttacgaag ttgctgctgt ttcttctgac 480

gctgttctga ccccgtacca cgctgttcac aaagttaaac cggacctggt tccgaccgct 540

aaaatcctgg ttatgggtct gggtggtctg ggttctaacg ctgttcagat catcaaaaac 600

tacggttgcc acgttgttgc ttgcgacgtt aaaccggaac tggaagaatt cgctcgtcag 660

tgcggtgctg acgaattcta caccgacatc aactcttctc cgcacaaacc ggaatctttc 720

gacgtttgct tcgacttcgt ttctttccag gaaaccttcg acgtttgcca gaaatacgct 780

cagtctggtg gtaaaatcgt tgttgttggt ctgggtcgtt ctaaactgat gttccgtaac 840

tacgacctgg ctcgtcgttc tctgcaggtt atcttctctt tcggtggtac cgcttcttct 900

caggaacagt ctctgcagct ggtttctaaa ggtctggtta aaccgatggt taccaacggt 960

gacttcgctg aactgccgca gtacctgaaa cgtctggcta aaggtgaagt taaaggtcgt 1020

gttgttttcc gtccgtctaa actgtaa 1047

<210> 4

<211> 348

<212> PRT

<213> Artificial sequence ()

<400> 4

Met Leu Pro Ser Ile Gln Thr Ala Tyr Thr Phe Lys Lys Gly Ser Arg

1 5 10 15

Glu Ile Val Arg Arg Asp Asp Trp Pro Val Pro His Pro Glu Asp Asn

20 25 30

Gln Val Leu Leu Lys Val Glu Ala Val Gly Leu Cys Met Ser Asp Leu

35 40 45

His Ile Leu Met Ala Gln Glu Thr His Val Pro Glu Thr Phe Val Met

50 55 60

Gly His Glu Ile Ala Gly Ser Ile Ala Ala Val Gly Gly Lys Leu Glu

65 70 75 80

Ser Asp Pro Arg Tyr Lys Val Gly Asn Arg Phe Thr Val Cys Ile Gly

85 90 95

Leu Thr Cys Gly Arg Cys Ala Asn Cys Arg Asn Gly His Asp Asn Cys

100 105 110

Cys Thr Gly Asn Gly Lys Phe Pro Gly Ala Tyr Gly Leu Asn Arg Asp

115 120 125

Gly Gly Phe Gln Gln Tyr Leu Leu Val Thr Asp Leu Asn Thr Leu Leu

130 135 140

Pro Ile Pro Asp Gly Leu Ser Tyr Glu Val Ala Ala Val Ser Ser Asp

145 150 155 160

Ala Val Leu Thr Pro Tyr His Ala Val His Lys Val Lys Pro Asp Leu

165 170 175

Val Pro Thr Ala Lys Ile Leu Val Met Gly Leu Gly Gly Leu Gly Ser

180 185 190

Asn Ala Val Gln Ile Ile Lys Asn Tyr Gly Cys His Val Val Ala Cys

195 200 205

Asp Val Lys Pro Glu Leu Glu Glu Phe Ala Arg Gln Cys Gly Ala Asp

210 215 220

Glu Phe Tyr Thr Asp Ile Asn Ser Ser Pro His Lys Pro Glu Ser Phe

225 230 235 240

Asp Val Cys Phe Asp Phe Val Ser Phe Gln Glu Thr Phe Asp Val Cys

245 250 255

Gln Lys Tyr Ala Gln Ser Gly Gly Lys Ile Val Val Val Gly Leu Gly

260 265 270

Arg Ser Lys Leu Met Phe Arg Asn Tyr Asp Leu Ala Arg Arg Ser Leu

275 280 285

Gln Val Ile Phe Ser Phe Gly Gly Thr Ala Ser Ser Gln Glu Gln Ser

290 295 300

Leu Gln Leu Val Ser Lys Gly Leu Val Lys Pro Met Val Thr Asn Gly

305 310 315 320

Asp Phe Ala Glu Leu Pro Gln Tyr Leu Lys Arg Leu Ala Lys Gly Glu

325 330 335

Val Lys Gly Arg Val Val Phe Arg Pro Ser Lys Leu

340 345

<210> 5

<211> 1047

<212> DNA

<213> Artificial sequence ()

<400> 5

atgctgccgt ctatccagac cgcttacacc ttcaaacgtg gttctcgtga aatcgttcgt 60

cgtgacgact ggccggttcc gcacccggaa gacaaccagg ttctgctgaa agttgaagct 120

gttggtctgt gcatgtctga cctgcacatc ctgatggctc aggaaaccca cgttccggaa 180

accttcgtta tgggtcacga aatcgctggt tctatcgctg ctgttggtga caaactggaa 240

tctgacccgc gttacaaagt tggtaaccgt ttcgctgttt gcatcggtaa cacctgcggt 300

cgttgcgcta actgccgtaa cggtcacgac aactgctgca ccggtaacgg taaattcccg 360

ggtgcttacg gtctgaaccg tgacggtggt taccagcagt acctgctggt taccgacctg 420

aacaccctgc tgccgatccc ggacaacgtt tcttacgaag ttgctgctgc ttcttctgac 480

gctgttctga ccccgtacca cgctatcaaa aaagttaaac cggacgttgt tccgacctct 540

aaaatcctgg ttatgggtct gggtggtctg ggttctaacg ctgttcagat catcaaagct 600

ttcggtgctc acgttgttgc ttgcgacgtt aaaccggaac tggaagaatt cgctcgtcag 660

tgcggtgctg acgaattcta caccgacatc aactcttctc cgcacaaacc ggaatctttc 720

gacgtttgct tcgacttctg cggtttccag gaaaccttcg acgtttgcca gaaatacgct 780

cagtctggtg gtaaaatcgt tgttgttggt ctgggtcgtt ctaaactgat gctgcgtaac 840

tacgactctg ctcgtcgttc tctgcaggtt atcttctctt tctggggtac cgcttcttct 900

caggaacagt ctctgcagtg ggttaccaaa ggtctggtta aaccgatggt taccaacggt 960

gacttcgctg aactgccgga atacctggaa aaactggcta aaggtgaagt taaaggtcgt 1020

gttgttttcc gtccgtctaa actgtaa 1047

<210> 6

<211> 348

<212> PRT

<213> Artificial sequence ()

<400> 6

Met Leu Pro Ser Ile Gln Thr Ala Tyr Thr Phe Lys Arg Gly Ser Arg

1 5 10 15

Glu Ile Val Arg Arg Asp Asp Trp Pro Val Pro His Pro Glu Asp Asn

20 25 30

Gln Val Leu Leu Lys Val Glu Ala Val Gly Leu Cys Met Ser Asp Leu

35 40 45

His Ile Leu Met Ala Gln Glu Thr His Val Pro Glu Thr Phe Val Met

50 55 60

Gly His Glu Ile Ala Gly Ser Ile Ala Ala Val Gly Asp Lys Leu Glu

65 70 75 80

Ser Asp Pro Arg Tyr Lys Val Gly Asn Arg Phe Ala Val Cys Ile Gly

85 90 95

Asn Thr Cys Gly Arg Cys Ala Asn Cys Arg Asn Gly His Asp Asn Cys

100 105 110

Cys Thr Gly Asn Gly Lys Phe Pro Gly Ala Tyr Gly Leu Asn Arg Asp

115 120 125

Gly Gly Tyr Gln Gln Tyr Leu Leu Val Thr Asp Leu Asn Thr Leu Leu

130 135 140

Pro Ile Pro Asp Asn Val Ser Tyr Glu Val Ala Ala Ala Ser Ser Asp

145 150 155 160

Ala Val Leu Thr Pro Tyr His Ala Ile Lys Lys Val Lys Pro Asp Val

165 170 175

Val Pro Thr Ser Lys Ile Leu Val Met Gly Leu Gly Gly Leu Gly Ser

180 185 190

Asn Ala Val Gln Ile Ile Lys Ala Phe Gly Ala His Val Val Ala Cys

195 200 205

Asp Val Lys Pro Glu Leu Glu Glu Phe Ala Arg Gln Cys Gly Ala Asp

210 215 220

Glu Phe Tyr Thr Asp Ile Asn Ser Ser Pro His Lys Pro Glu Ser Phe

225 230 235 240

Asp Val Cys Phe Asp Phe Cys Gly Phe Gln Glu Thr Phe Asp Val Cys

245 250 255

Gln Lys Tyr Ala Gln Ser Gly Gly Lys Ile Val Val Val Gly Leu Gly

260 265 270

Arg Ser Lys Leu Met Leu Arg Asn Tyr Asp Ser Ala Arg Arg Ser Leu

275 280 285

Gln Val Ile Phe Ser Phe Trp Gly Thr Ala Ser Ser Gln Glu Gln Ser

290 295 300

Leu Gln Trp Val Thr Lys Gly Leu Val Lys Pro Met Val Thr Asn Gly

305 310 315 320

Asp Phe Ala Glu Leu Pro Glu Tyr Leu Glu Lys Leu Ala Lys Gly Glu

325 330 335

Val Lys Gly Arg Val Val Phe Arg Pro Ser Lys Leu

340 345

<210> 7

<211> 323

<212> PRT

<213> Sporidiobolus salmonicolor

<400> 7

Met Val Gly Thr Thr Thr Leu Asn Thr Gly Ala Ser Leu Glu Leu Val

1 5 10 15

Gly Tyr Gly Thr Trp Gln Ala Ala Pro Gly Glu Val Gly Gln Gly Val

20 25 30

Lys Val Ala Ile Glu Thr Gly Tyr Arg His Leu Asp Leu Ala Lys Val

35 40 45

Tyr Ser Asn Gln Pro Glu Val Gly Ala Ala Ile Lys Glu Ala Gly Val

50 55 60

Lys Arg Glu Asp Leu Phe Ile Thr Ser Lys Leu Trp Asn Asn Ser His

65 70 75 80

Arg Pro Glu Gln Val Glu Pro Ala Leu Asp Asp Thr Leu Lys Glu Leu

85 90 95

Gly Leu Glu Tyr Leu Asp Leu Tyr Leu Ile His Trp Pro Val Ala Phe

100 105 110

Pro Pro Glu Gly Asp Ile Thr Gln Asn Leu Phe Pro Lys Ala Asn Asp

115 120 125

Lys Glu Val Lys Leu Asp Leu Glu Val Ser Leu Val Asp Thr Trp Lys

130 135 140

Ala Met Val Lys Leu Leu Asp Thr Gly Lys Val Lys Ala Ile Gly Val

145 150 155 160

Ser Asn Phe Asp Ala Lys Met Val Asp Ala Ile Ile Glu Ala Thr Gly

165 170 175

Val Thr Pro Ser Val Asn Gln Ile Glu Arg His Pro Leu Leu Leu Gln

180 185 190

Pro Glu Leu Ile Ala His His Lys Ala Lys Asn Ile His Ile Thr Ala

195 200 205

Tyr Ser Pro Leu Gly Asn Asn Thr Val Gly Ala Pro Leu Leu Val Gln

210 215 220

His Pro Glu Ile Lys Arg Ile Ala Glu Lys Asn Gly Cys Thr Pro Ala

225 230 235 240

Gln Val Leu Ile Ala Trp Ala Ile Val Gly Gly His Ser Val Ile Pro

245 250 255

Lys Ser Val Thr Pro Ser Arg Ile Gly Glu Asn Phe Lys Gln Val Ser

260 265 270

Leu Ser Gln Glu Asp Val Asp Ala Val Ser Lys Leu Gly Glu Gly Ser

275 280 285

Gly Arg Arg Arg Tyr Asn Ile Pro Cys Thr Tyr Ser Pro Lys Trp Asp

290 295 300

Ile Asn Val Phe Gly Glu Glu Asp Glu Lys Ser Cys Lys Asn Ala Val

305 310 315 320

Lys Ile Lys

<210> 8

<211> 348

<212> PRT

<213> Meyerozyma guilliermondii

<400> 8

Met Leu Pro Ser Ile Gln Thr Ala Tyr Thr Phe Lys Arg Gly Ser Arg

1 5 10 15

Glu Ile Val Arg Arg Asp Asp Trp Pro Val Pro His Pro Glu Asp Asn

20 25 30

Gln Val Leu Leu Lys Val Glu Ala Val Gly Leu Cys Met Ser Asp Val

35 40 45

His Ile Leu Met Ala Gln Glu Thr His Val Pro Glu Thr Phe Val Met

50 55 60

Gly His Glu Ile Ala Gly Ser Ile Ala Ala Val Gly Gly Lys Leu Glu

65 70 75 80

Ser Asp Pro Arg Tyr Lys Val Gly Asn Arg Phe Thr Val Cys Ile Gly

85 90 95

Leu Thr Cys Gly Arg Cys Ala Asn Cys Arg Asn Gly His Asp Asn Cys

100 105 110

Cys Thr Gly Asn Gly Lys Phe Pro Gly Ala Tyr Gly Leu Asn Arg Asp

115 120 125

Gly Gly Phe Gln Gln Tyr Leu Leu Val Thr Asp Leu Asn Thr Leu Leu

130 135 140

Pro Leu Pro Asp Gly Leu Ser Tyr Glu Met Ala Ala Val Ser Ser Asp

145 150 155 160

Ser Val Leu Thr Pro Leu His Ala Val His Lys Val Lys Pro Asp Leu

165 170 175

Val Pro Thr Ala Lys Ile Leu Val Met Gly Leu Gly Gly Leu Gly Ser

180 185 190

Asn Ala Val Gln Ile Ile Lys Asn Tyr Gly Cys His Val Val Ala Cys

195 200 205

Asp Val Lys Pro Glu Leu Glu Glu Phe Ala Arg Gln Cys Gly Ala Asp

210 215 220

Glu Phe Tyr Thr Asp Ile Asn Ser Ser Pro His Lys Pro Glu Ser Phe

225 230 235 240

Asp Val Cys Phe Asp Phe Cys Gly Phe Gln Glu Thr Phe Asp Ser Cys

245 250 255

Gln Lys Tyr Ala Gln Ser Gly Gly Lys Ile Val Val Val Gly Leu Gly

260 265 270

Arg Ser Lys Leu Met Leu Arg Asn Tyr Asp Ser Ala Arg Arg Ser Leu

275 280 285

Gln Val Ile Phe Ser Phe Gly Gly Thr Ala Ser Ser Gln Glu Gln Ser

290 295 300

Leu Gln Trp Val Thr Lys Gly Leu Val Lys Pro Met Val Thr Asn Gly

305 310 315 320

Asp Phe Ala Glu Leu Pro Gln Tyr Leu Lys Arg Leu Ala Lys Gly Glu

325 330 335

Val Lys Gly Arg Val Val Phe Arg Pro Ser Lys Leu

340 345

Claims (6)

1. A ketoreductase mutant with improved enzyme activity is characterized in that the sequence of the ketoreductase mutant is SEQ ID NO. 6.

2. A polynucleotide encoding a ketoreductase mutant having the sequence of SEQ ID No. 6.

3. A polynucleotide according to claim 2, wherein the sequence of the polynucleotide is SEQ ID No. 5.

4. A recombinant plasmid comprising an expression vector having a polynucleotide having the sequence of SEQ ID No.5 linked thereto.

5. A host cell comprising the recombinant plasmid of claim 4.

6. A host cell according to claim 5, wherein the cell is an E.coli cell.

Images