CN111454309A - Extraction method of saffron petal anthocyanin - Google Patents
Extraction method of saffron petal anthocyanin Download PDFInfo
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- CN111454309A CN111454309A CN202010409354.7A CN202010409354A CN111454309A CN 111454309 A CN111454309 A CN 111454309A CN 202010409354 A CN202010409354 A CN 202010409354A CN 111454309 A CN111454309 A CN 111454309A
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- saffron
- extraction
- extracting
- anthocyanin
- petals
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
- C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
- C07H17/06—Benzopyran radicals
- C07H17/065—Benzo[b]pyrans
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
- C09B67/0096—Purification; Precipitation; Filtration
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- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a method for extracting saffron petal anthocyanin, which comprises the following steps: taking saffron petals, crushing, adding an ethanol solution with the ethanol volume percentage of 20-90%, extracting, taking an extracting solution, and drying to obtain the saffron health care product. Under the specific extraction conditions of the invention, the yield of saffron petal anthocyanin in the obtained extract is obviously improved. The extraction method provided by the invention is simple, mild in condition, easy to operate, excellent in extraction effect and suitable for expanded production.
Description
Technical Field
The invention belongs to the field of medicine extraction, and particularly relates to a method for extracting saffron petal anthocyanin.
Background
Modern researches show that the saffron can prolong the blood coagulation time, inhibit platelet aggregation induced by adenosine diphosphate and collagen, and enhance the fibrinolytic activity, and can be used for preventing and treating cardiovascular system diseases such as coronary heart disease, angina pectoris and the like.
Saffron anthocyanin, also called saffron anthocyanin, is a pigment extracted from saffron. Research shows that the crocin anthocyanin is an antioxidant substance, can maintain normal cell connection and blood vessel stability, enhance the circulation of micro blood vessels, improve the flow of the micro blood vessels and veins, and has good effects of enhancing vision and eliminating eye fatigue. The Chinese patent application CN201210426A discloses a formula of a health product for protecting eyesight, which is prepared by mixing crocin and anthocyanin, can effectively improve the blood circulation of eyes, protect retina from being damaged by visible light and ultraviolet light, can effectively protect eyes, prevent and reduce the generation of myopia, hypermetropia and amblyopia, and is widely suitable for various crowds. However, the patent application does not disclose a process for extracting crocin from crocus sativus, and other documents are not reported.
Therefore, the research of the technology for extracting the saffron anthocyanin, which has the advantages of mild condition, easy operation and high extraction rate, is suitable for expanded production and has important significance for preparing medicaments for enhancing eyesight and the like.
Disclosure of Invention
The invention aims to provide a method for extracting saffron petal anthocyanin with high extraction rate.
The invention provides a method for extracting saffron petal anthocyanin, which comprises the following steps: taking saffron petals, crushing, adding an ethanol solution with the ethanol volume percentage of 20-90%, extracting, taking an extracting solution, and drying to obtain the saffron health care product.
Further, the volume percentage of ethanol in the ethanol solution is 50-70%, preferably 50%.
Furthermore, the mass volume ratio of the saffron petals to the ethanol solution is 1 (20-120) g/m L.
Furthermore, the mass volume ratio of the saffron petals to the ethanol solution is 1 (20-60) g/m L.
Further, the mass-volume ratio of the saffron petals to the ethanol solution is 1: 40g/m L.
Further, the extraction temperature is 25-80 ℃.
Further, the extraction temperature is 50-60 ℃, and preferably 60 ℃.
Further, the extraction time is 15-25 min, preferably 20 min.
Further, the extraction mode is ultrasonic extraction; preferably, the ultrasonic power during ultrasonic extraction is 200-600W, and the ultrasonic frequency is 30-70 kHz; more preferably, the ultrasonic power during ultrasonic extraction is 300W, and the ultrasonic frequency is 50 kHz.
Further, the saffron petals are dry saffron petals.
The experimental result shows that under the specific extraction condition of the invention, the yield of the saffron petal anthocyanin in the obtained extract is obviously improved. The extraction method provided by the invention is simple, mild in condition, easy to operate, excellent in extraction effect and suitable for expanded production.
Obviously, many modifications, substitutions, and variations are possible in light of the above teachings of the invention, without departing from the basic technical spirit of the invention, as defined by the following claims.
The present invention will be described in further detail with reference to the following examples. This should not be understood as limiting the scope of the above-described subject matter of the present invention to the following examples. All the technologies realized based on the above contents of the present invention belong to the scope of the present invention.
Detailed Description
The raw materials and equipment used in the invention are known products and are obtained by purchasing commercial products.
Example 1 extraction Process of saffron petal anthocyanin
Taking dry saffron petals (1.0015g), crushing, sieving by a third pharmacopoeia sieve to obtain petal powder, extracting the petal powder for 20min under the ultrasonic condition of 60 ℃ by using a 50% ethanol solution with the volume percentage of ethanol, wherein the volume of the ethanol solution is 1: 40g/m L according to the mass of the petals, and distilling the obtained extract under reduced pressure until no alcohol smell exists, standing the extract in a refrigerator at the temperature of-20 ℃ for freezing overnight, and then freeze-drying the extract until the weight is constant to obtain 0.5743g of crude saffron petal anthocyanin extract.
The beneficial effects of the extraction process of the present invention are demonstrated by the following experimental examples.
Experimental example 1 Process screening experiment
1. Experimental methods
With reference to the extraction process of example 1, the volume percentage of ethanol in the ethanol solution, the volume ratio of petal mass to ethanol solution, and the extraction temperature were changed according to the parameters shown in table 1, and extraction was performed for 20min under ultrasonic conditions to obtain each extract;
diluting the above extractive solutions by 10 times by ultraviolet spectrophotometry, scanning maximum absorption wavelength, detecting absorbance at the maximum absorption wavelength, and calculating the obtained saffron petal anthocyanin yield (calculated as pelargonidin-3-glucoside) according to the absorbance:
the yield of anthocyanin in saffron petals is A × M × F × V/(×L× M)
In the formula, A is an absorbance value of an extracting solution at the maximum absorption wavelength, M is the molar molecular weight of pelargonidin-3-glucoside, F is a dilution multiple, the molar extinction coefficient of pelargonidin-3-glucoside is 22400L/(mol cm), V is the volume of the extracting solution, M L, L is the thickness of a cuvette, 1cm, and M is the mass of saffron petals, g.
2. Results of the experiment
TABLE 1 comparison of extraction results under various process parameters
As can be seen from Table 1, according to the extraction method provided by the invention, when the volume ratio of the petal mass to the ethanol solution is 1: 40g/m L, the extraction temperature is 50-60 ℃, and the volume percentage of ethanol in the ethanol solution is 50-70%, the yield of the obtained saffron petal anthocyanin reaches 4.367228721-4.728844548%, and the extraction effect is remarkably superior to that of other conditions.
In conclusion, the invention provides a method for extracting saffron petal anthocyanin from saffron petals, and the yield of the saffron petal anthocyanin in the obtained extract is obviously improved under the specific extraction conditions of the invention. The extraction method provided by the invention is simple, mild in condition, easy to operate, excellent in extraction effect and suitable for expanded production.
Claims (10)
1. A method for extracting saffron petal anthocyanin is characterized by comprising the following steps: the method comprises the following steps: taking saffron petals, crushing, adding an ethanol solution with the ethanol volume percentage of 20-90%, extracting, taking an extracting solution, and drying to obtain the saffron health care product.
2. The method of claim 1, wherein: the volume percentage of the ethanol in the ethanol solution is 50-70%, preferably 50%.
3. The method according to claim 1 or 2, wherein the mass-to-volume ratio of the saffron petals to the ethanol solution is 1 (20-120) g/m L.
4. The method as claimed in claim 3, wherein the mass-to-volume ratio of the saffron petals to the ethanol solution is 1 (20-60) g/m L.
5. The method as claimed in claim 4, wherein the mass-to-volume ratio of saffron petals to the ethanol solution is 1: 40g/m L.
6. The method according to any one of claims 1 to 5, wherein: the extraction temperature is 25-80 ℃.
7. The method of claim 6, wherein: the extraction temperature is 50-60 ℃, and preferably 60 ℃.
8. The method according to any one of claims 1 to 7, wherein: the extraction time is 15-25 min, preferably 20 min.
9. The method according to any one of claims 1 to 7, wherein: the extraction mode is ultrasonic extraction; preferably, the ultrasonic power during ultrasonic extraction is 200-600W, and the ultrasonic frequency is 30-70 kHz; more preferably, the ultrasonic power during ultrasonic extraction is 300W, and the ultrasonic frequency is 50 kHz.
10. The method according to any one of claims 1 to 7, wherein: the saffron petals are dry saffron petals.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010409354.7A CN111454309A (en) | 2020-05-14 | 2020-05-14 | Extraction method of saffron petal anthocyanin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010409354.7A CN111454309A (en) | 2020-05-14 | 2020-05-14 | Extraction method of saffron petal anthocyanin |
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| CN111454309A true CN111454309A (en) | 2020-07-28 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CH710983A2 (en) * | 2015-04-17 | 2016-10-31 | Tagora Ip Ag | Cosmetic composition with saffron petal leaf extract. |
| CN108026382A (en) * | 2015-06-30 | 2018-05-11 | 马斯公司 | Dye compositions and its application method based on acylated anthocyanin |
| CN109843314A (en) * | 2016-07-28 | 2019-06-04 | 活力因赛德公司 | The highly concentrated plant extracts of safranal, its production method and purposes |
-
2020
- 2020-05-14 CN CN202010409354.7A patent/CN111454309A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CH710983A2 (en) * | 2015-04-17 | 2016-10-31 | Tagora Ip Ag | Cosmetic composition with saffron petal leaf extract. |
| CN108026382A (en) * | 2015-06-30 | 2018-05-11 | 马斯公司 | Dye compositions and its application method based on acylated anthocyanin |
| CN109843314A (en) * | 2016-07-28 | 2019-06-04 | 活力因赛德公司 | The highly concentrated plant extracts of safranal, its production method and purposes |
Non-Patent Citations (5)
| Title |
|---|
| L. LOTFI，等: "Effects of sulfur water extraction on anthocyanins properties of tepals in flower of saffron (Crocus sativus L)" * |
| 王伟华;曹员;刘飞;: "慕萨莱思酒中原花青素提取与纯化条件的优化" * |
| 范瑞霞，等: "响应面法在葡萄果皮色素超声辅助提取工艺中的应用" * |
| 闫征，等: "大孔树脂分离纯化草莓中花色苷工艺研究" * |
| 陈娜，等: "西红花非药用部位化学成分和药理作用研究进展" * |
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