CN111453163A - Rapid fluorescent quantitative PCR detection kit for porcine reproductive and respiratory syndrome virus - Google Patents

Rapid fluorescent quantitative PCR detection kit for porcine reproductive and respiratory syndrome virus Download PDF

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Publication number
CN111453163A
CN111453163A CN202010286419.3A CN202010286419A CN111453163A CN 111453163 A CN111453163 A CN 111453163A CN 202010286419 A CN202010286419 A CN 202010286419A CN 111453163 A CN111453163 A CN 111453163A
Authority
CN
China
Prior art keywords
box body
foam
reagent bottle
respiratory syndrome
syndrome virus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202010286419.3A
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Chinese (zh)
Inventor
汪劲能
陈菲菲
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanghai Xiongtu Biotechnology Co ltd
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Shanghai Xiongtu Biotechnology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shanghai Xiongtu Biotechnology Co ltd filed Critical Shanghai Xiongtu Biotechnology Co ltd
Priority to CN202010286419.3A priority Critical patent/CN111453163A/en
Publication of CN111453163A publication Critical patent/CN111453163A/en
Pending legal-status Critical Current

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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D25/00Details of other kinds or types of rigid or semi-rigid containers
    • B65D25/02Internal fittings
    • B65D25/10Devices to locate articles in containers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D81/00Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
    • B65D81/02Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents specially adapted to protect contents from mechanical damage
    • B65D81/05Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents specially adapted to protect contents from mechanical damage maintaining contents at spaced relation from package walls, or from other contents
    • B65D81/107Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents specially adapted to protect contents from mechanical damage maintaining contents at spaced relation from package walls, or from other contents using blocks of shock-absorbing material
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D85/00Containers, packaging elements or packages, specially adapted for particular articles or materials
    • B65D85/30Containers, packaging elements or packages, specially adapted for particular articles or materials for articles particularly sensitive to damage by shock or pressure
    • B65D85/302Containers, packaging elements or packages, specially adapted for particular articles or materials for articles particularly sensitive to damage by shock or pressure for carboys

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  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a rapid fluorescent quantitative PCR detection kit for porcine reproductive and respiratory syndrome virus, which comprises an outer box body, a reagent bottle and an inner box body, wherein the inner box body is arranged inside the outer box body, second foam is fixed at the inner bottom of the inner box body, a stabilizing mechanism is arranged on the second foam, the stabilizing mechanism comprises a rubber block, an extension groove and a placing groove, and the surface of the second foam is provided with the placing groove for the reagent bottle to be inserted; the invention has the beneficial effects that: through the designed stabilizing mechanism, when the reagent bottle storage device is used, a reagent bottle for storing a reagent penetrates through the penetrating opening formed in the surface of the rubber block in a penetrating manner, and the bottom of the reagent bottle is placed in the placing groove to play a role in stably supporting the reagent bottle; the medial surface fastening of outer box has the first foam of symmetric distribution, and the tip of first foam can offset with interior box body, utilizes first foam and interior box body to offset, plays the firm effect when placing internal box body, when reducing the removal kit, rocks of interior box body.

Description

Rapid fluorescent quantitative PCR detection kit for porcine reproductive and respiratory syndrome virus
Technical Field
The invention belongs to the technical field of PCR detection kits, and particularly relates to a rapid fluorescent quantitative PCR detection kit for porcine reproductive and respiratory syndrome virus.
Background
Porcine reproductive and respiratory syndrome virus is a highly contagious disease caused by porcine reproductive and respiratory syndrome virus characterized by fever, anorexia, late abortion, premature birth, stillbirth, weak fetus and mummy fetus in infected pigs, and respiratory disorders in pigs of various ages, particularly piglets.
Mycoplasma in the live non-culturable (VBNC) state cannot be detected using conventional Mycoplasma culture methods and there is also a risk that false negative results may be obtained. The PCR kit provides a method for early detection of mycoplasma contamination. These kits are rapid, highly specific, sensitive, and meet international guidelines.
The existing rapid fluorescent primer PCR detection kit for the porcine reproductive and respiratory syndrome virus has the following defects when in use:
there is a deficiency in increasing the stability of reagent bottle placement.
Disclosure of Invention
The invention aims to provide a rapid fluorescence quantitative PCR detection kit for porcine reproductive and respiratory syndrome virus, which solves the problem that the stability of placing a reagent bottle is not enough in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a quick porcine reproductive and respiratory syndrome virus fluorescence quantitative PCR detect reagent box, includes outer box body, reagent bottle, interior box body, the inside of outer box body is arranged in to interior box body, and the interior bottom of this interior box body is fixed with the second foam, be provided with firm mechanism on the second foam, this firm mechanism includes rubber piece, extending groove and standing groove, the surface of second foam is seted up and is supplied reagent bottle male standing groove, the extending groove has been seted up to the notch department of standing groove, the extending groove is put on the shelf and is equipped with the rubber piece, the inside that the rubber piece can be followed to the reagent bottle passes.
Preferably, the cross section of the rubber block is annular, and a through opening is formed in the surface of the rubber block in a penetrating mode.
Preferably, the placing grooves are provided in plurality and are evenly distributed.
Preferably, the outer box is provided with an outer box top cover.
Preferably, the outer box body top cover and the inner box body are all processed by white cardboard.
Preferably, the inner side surface of the outer box body is fastened with first foams which are symmetrically distributed, and the end parts of the first foams can be abutted against the inner box body.
Compared with the prior art, the invention has the beneficial effects that:
(1) through the designed stabilizing mechanism, when the reagent bottle storage device is used, a reagent bottle for storing a reagent penetrates through the penetrating opening formed in the surface of the rubber block in a penetrating manner, and the bottom of the reagent bottle is placed in the placing groove to play a role in stably supporting the reagent bottle;
(2) the medial surface fastening of outer box has the first foam of symmetric distribution, and the tip of first foam can offset with interior box body, utilizes first foam and interior box body to offset, plays the firm effect when placing internal box body, when reducing the removal kit, rocks of interior box body.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is an enlarged view of the Y-region of FIG. 1 according to the present invention;
FIG. 3 is a schematic perspective view of a rubber block according to the present invention;
in the figure: 1. an outer case; 2. a reagent bottle; 3. a top cover of the outer box body; 4. an inner box body; 5. a first foam; 6. a second foam; 7. a rubber block; 8. an extension groove; 9. and (6) placing the groove.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1, fig. 2 and fig. 3, the present invention provides a technical solution: a rapid fluorescence quantitative PCR detection kit for porcine reproductive and respiratory syndrome virus comprises an outer box body 1, a reagent bottle 2 and an inner box body 4, wherein the inner box body 4 is arranged in the outer box body 1, a second foam 6 is fixed at the inner bottom of the inner box body 4, a stabilizing mechanism is arranged on the second foam 6 and comprises a rubber block 7, an extending groove 8 and a placing groove 9, the placing groove 9 for inserting the reagent bottle 2 is formed in the surface of the second foam 6, the extending groove 8 is formed in the notch of the placing groove 9, the rubber block 7 is erected on the extending groove 8, the cross section of the rubber block 7 is annular, a through hole is formed in the surface of the rubber block 7 in a penetrating manner, the reagent bottle 2 can conveniently penetrate through the through hole of the rubber block 7, and the reagent bottle 2 is limited; during the use, to deposit reagent bottle 2 of reagent, run through the mouth of wearing of seting up from the rubber block 7 surface and pass to make reagent bottle 2's bottom place in standing groove 9, play the firm support to reagent bottle 2.
In this embodiment, preferably, the placing grooves 9 are provided in a plurality, and the placing grooves 9 are uniformly distributed, so as to facilitate storage of the reagent bottles 2.
In this embodiment, it is preferable that the outer case 1 is provided with an outer case lid 3, and the outer case 1 is protected by the outer case lid 3.
In this embodiment, preferably, the outer box 1, the outer box top cover 3, and the inner box 4 are all made of white cardboard, which facilitates the use of the kit.
In this embodiment, preferred, the medial surface fastening of outer box 1 has the first foam 5 of symmetric distribution, and the tip of first foam 5 can offset with interior box body 4, utilizes first foam 5 and interior box body 4 to offset, plays the firm effect when placing interior box body 4, and when reducing the removal kit, interior box body 4 rocks.
The working principle and the using process of the invention are as follows: the top cover 3 of the outer box body is opened, the first foam 5 is fastened on the inner side surface of the outer box body 1, the inner box body 4 is arranged inside the outer box body 1, the end part of the first foam 5 can abut against the inner box body 4, the first foam 5 abuts against the inner box body 4, the stabilizing effect when the inner box body 4 is arranged is achieved, and shaking of the inner box body 4 when the kit is moved is reduced;
when the reagent bottle holder is used, the reagent bottle 2 for storing the reagent penetrates through the penetrating opening formed in the surface of the rubber block 7, and the bottom of the reagent bottle 2 is placed in the placing groove 9 to stably support the reagent bottle 2;
and taking out the reagent in the reagent bottle 2 to detect the porcine reproductive and respiratory syndrome virus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (6)

1. The utility model provides a quick porcine reproductive and respiratory syndrome virus fluorescence quantitative PCR detect reagent box, includes outer box body (1), reagent bottle (2), interior box body (4), the inside of outer box body (1) is arranged in to interior box body (4), and the interior bottom of this interior box body (4) is fixed with second foam (6), its characterized in that: be provided with firm mechanism on second foam (6), this firm mechanism includes block rubber (7), extension (8) and standing groove (9), supply reagent bottle (2) male standing groove (9) have been seted up on the surface of second foam (6), extension (8) have been seted up to the notch department of standing groove (9), extension (8) have been put on the shelf and have been equipped with block rubber (7), inside that block rubber (7) can be followed to reagent bottle (2) is passed.
2. The rapid fluorescent quantitative PCR detection kit for porcine reproductive and respiratory syndrome virus according to claim 1, which is characterized in that: the cross section of the rubber block (7) is annular, and a through opening is formed in the surface of the rubber block (7) in a penetrating mode.
3. The rapid fluorescent quantitative PCR detection kit for porcine reproductive and respiratory syndrome virus according to claim 1, which is characterized in that: the plurality of placing grooves (9) are arranged and uniformly distributed.
4. The rapid fluorescent quantitative PCR detection kit for porcine reproductive and respiratory syndrome virus according to claim 1, which is characterized in that: the outer box body (1) is provided with an outer box body top cover (3).
5. The rapid fluorescent quantitative PCR detection kit for porcine reproductive and respiratory syndrome virus according to claim 4, which is characterized in that: the outer box body (1), the outer box body top cover (3) and the inner box body (4) are all formed by processing white cardboard.
6. The rapid fluorescent quantitative PCR detection kit for porcine reproductive and respiratory syndrome virus according to claim 1, which is characterized in that: the inner side surface of the outer box body (1) is fastened with first foams (5) which are symmetrically distributed, and the end parts of the first foams (5) can be abutted against the inner box body (4).
CN202010286419.3A 2020-04-13 2020-04-13 Rapid fluorescent quantitative PCR detection kit for porcine reproductive and respiratory syndrome virus Pending CN111453163A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010286419.3A CN111453163A (en) 2020-04-13 2020-04-13 Rapid fluorescent quantitative PCR detection kit for porcine reproductive and respiratory syndrome virus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010286419.3A CN111453163A (en) 2020-04-13 2020-04-13 Rapid fluorescent quantitative PCR detection kit for porcine reproductive and respiratory syndrome virus

Publications (1)

Publication Number Publication Date
CN111453163A true CN111453163A (en) 2020-07-28

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CN202010286419.3A Pending CN111453163A (en) 2020-04-13 2020-04-13 Rapid fluorescent quantitative PCR detection kit for porcine reproductive and respiratory syndrome virus

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CN (1) CN111453163A (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11227775A (en) * 1998-02-18 1999-08-24 Taisei Corp Storage case for reagent bottle or the like
CN203946384U (en) * 2014-07-14 2014-11-19 王识博 A kind of experiment reagent case
CN205256899U (en) * 2015-12-14 2016-05-25 重庆博士泰生物技术有限公司 Reagent packing carton
CN206537648U (en) * 2017-03-15 2017-10-03 广东国盛医学科技有限公司 One kind tool shockproof kit with dissection
CN207748208U (en) * 2017-10-20 2018-08-21 深圳市金其美医疗器械有限公司 A kind of new medical kit

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11227775A (en) * 1998-02-18 1999-08-24 Taisei Corp Storage case for reagent bottle or the like
CN203946384U (en) * 2014-07-14 2014-11-19 王识博 A kind of experiment reagent case
CN205256899U (en) * 2015-12-14 2016-05-25 重庆博士泰生物技术有限公司 Reagent packing carton
CN206537648U (en) * 2017-03-15 2017-10-03 广东国盛医学科技有限公司 One kind tool shockproof kit with dissection
CN207748208U (en) * 2017-10-20 2018-08-21 深圳市金其美医疗器械有限公司 A kind of new medical kit

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Application publication date: 20200728