CN111445957B - Mother and infant flora transfer monitoring system and method - Google Patents
Mother and infant flora transfer monitoring system and method Download PDFInfo
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- 238000011156 evaluation Methods 0.000 claims abstract description 81
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Abstract
A maternal and infant flora transfer monitoring system and method divide intestinal flora of pregnant and lying-in women and newborns into healthy flora, missing flora and pathogenic flora through a pregnant and lying-in women flora monitoring and evaluating subsystem and a newborns flora monitoring and evaluating subsystem so as to carry out standardized management on transfer of maternal and infant flora, and carry out effective tracking management on the intestinal flora of the pregnant and lying-in women and newborns through flora evaluation, thereby providing important data support for individual nutrition recommendation and health management.
Description
Technical Field
The invention belongs to the technical field of human microbiome, and relates to a maternal and infant flora transmission monitoring system and method.
Background
Various researches on genetics, biology and immunology show that, as the neonate of caesarean section does not pass through the maternal birth canal, the maternal flora is not normally transferred to the neonate of caesarean section, so that the risk of various diseases such as obesity, allergy, asthma and the like of the neonate of caesarean section in the growing and developing process is increased. During the whole gestation period, the current hospital does not detect and distinguish the flora of the pregnant and lying-in women, especially for the pregnant and lying-in women suffering from chronic diseases such as gestational diabetes mellitus, if the flora is diseased, bad flora can be brought to the neonate to influence the healthy development of the neonate, so that the flora is monitored and intervened before the delivery of the pregnant and lying-in women, and the flora transferred to the neonate during the delivery can be kept healthy.
At present, for the transfer of maternal and infant flora of a parturient in caesarean section, there are some rags and the like for transferring maternal and infant flora on the market, vaginal secretion of the parturient is directly taken out through the rags to wipe a newborn, and the operation process is lack of standardization, so that adverse effects can be brought to infants or parturients.
Disclosure of Invention
In order to solve the problems, the invention provides a maternal and infant flora transfer monitoring system and a maternal and infant flora transfer monitoring method, which are used for carrying out standardized management on the transfer of maternal and infant flora, effectively monitoring and managing the maternal flora and the intestinal flora of newborns, and providing important data support for individual nutrition advice and health management.
The invention is realized by the following technical scheme:
The maternal and infant flora transfer monitoring system comprises a maternal and puerperal flora monitoring and evaluating subsystem, a neonatal flora monitoring and evaluating subsystem and a flora database, wherein the maternal and puerperal flora monitoring and evaluating subsystem and the neonatal flora monitoring and evaluating subsystem respectively comprise a flora acquisition module and a flora analysis module; the flora database is preset with flora types classified according to maternal and infant flora transfer health grades, the flora types can be updated in time and iteratively according to the maternal and infant microbiology and immunology researches in the continuous development of pregnancy and neonatal period, and the flora types include but are not limited to: a healthy group of bacteria, a deleted group of bacteria and a pathogenic group of bacteria;
the healthy flora comprises at least bacteroides, bifidobacteria and escherichia; the bifidobacterium genus at least comprises bifidobacterium longum and bifidobacterium breve, and the escherichia genus at least comprises escherichia coli; if the bacterium does not contain bacteroides, the bacterium is the deletion group; if enterobacter cloacae or klebsiella pneumoniae exists, the bacterium group is the pathogenic bacterium group;
The maternal and parturient flora monitoring and evaluating subsystem evaluates the flora according to the maternal and parturient flora acquired by the flora acquisition module, and the flora analysis module evaluates the flora in the flora database to obtain a flora type evaluation result of the maternal and parturient flora and gives related advice, and when the evaluation result is a healthy flora, advice which is continuously maintained and can be used for supplementing the neonatal flora is given; when the evaluation result is a missing flora, a suggestion that flora colonization supplementation is needed is given; when the evaluation result is a pathogenic bacteria group, advice which needs to be regulated and actively treated is given;
The system comprises a flora analysis module, a flora database, a flora analysis module, a community type evaluation module and a display module, wherein the flora analysis module is used for performing flora evaluation according to the initial flora of the neonate, which is acquired by the flora acquisition module, to obtain a flora type evaluation result of the initial flora of the neonate and give related advice, and finally the evaluation result and the related advice are displayed on the display module; when the evaluation result is a healthy flora, advice that flora supplementation is not needed is given; when the evaluation result is the deficiency flora, advice for flora supplementation is given; and when the evaluation result is a pathogenic bacteria group, advice that the flora is required to be supplemented and actively treated is given.
Preferably, the maternal and infant flora transmission monitoring system further comprises a maternal breast milk flora monitoring and evaluating subsystem, wherein the maternal breast milk flora monitoring and evaluating subsystem comprises a maternal breast milk flora acquisition module and a maternal breast milk flora analysis module, and the maternal breast milk flora monitoring and evaluating subsystem evaluates flora according to the maternal breast milk flora acquired by the maternal breast milk flora acquisition module and flora types in a flora database through the maternal breast milk flora analysis module to obtain a flora type evaluation result of the maternal breast milk flora and give related advice; when the evaluation result is a healthy bacterial group, giving a suggestion for continuing to keep; when the evaluation result is a deficiency group, advice which needs to be supplemented and regulated is given; and when the evaluation result is a pathogenic bacteria group, advice which needs to be regulated and actively treated is given.
Preferably, the neonatal initial flora comprises a neonatal period gestational flora.
Preferably, the maternal breast milk flora includes flora obtained by high-throughput sequencing of the maternal colostrum.
Preferably, the maternal bacterial flora comprises bacterial flora obtained by performing bacterial flora high-throughput sequencing on vaginal secretion of the maternal bacterial flora or bacterial flora obtained by performing bacterial flora high-throughput sequencing on maternal excrement.
Preferably, the maternal and infant flora transfer monitoring system further comprises a display module for displaying the evaluation result and the related advice, and a storage module for storing the maternal and maternal flora and the neonatal flora according to the settings of each user.
A flora collection auxiliary device for assisting the maternal and infant flora transfer monitoring system to collect flora comprises a sterile cleaning wet tissue for cleaning the periphery of a vagina, a sterile vaginal dilation clamp for draining vaginal secretions of a puerpera, medical sterile cotton for dipping the vaginal secretions, medical gloves, a culture dish for culturing flora, a soft cotton stick capable of extending into the oral cavity of a neonate and soft cotton gauze capable of being smeared on the neonate and breasts of the puerpera.
Preferably, the flora collection auxiliary device further comprises a heater for keeping the sterile cleaning wet tissue, the sterile vaginal dilation clip and the medical sterile cotton at the body temperature; and the auxiliary bag for producing is used for sealing and placing the sterile cleaning wet tissues, the sterile vaginal dilation clamp, medical sterile cotton, a culture dish, a soft cotton stick, soft cotton gauze and a heater.
The maternal and infant flora transfer monitoring method is characterized by comprising the following steps of:
Firstly, acquiring a maternal and lying-in woman flora by using the flora acquisition auxiliary device, namely acquiring the maternal and lying-in woman flora, and performing flora evaluation on the acquired maternal and lying-in woman intestinal flora by using the maternal and infant flora transmission monitoring system, and when the evaluation result is a healthy flora, giving a suggestion for continuous maintenance, and continuously acquiring and evaluating the flora to monitor the maternal and lying-in woman flora state; when the evaluation result is a deficiency flora, giving a suggestion to be regulated, and continuously collecting and evaluating flora after regulation to monitor the flora state of pregnant and lying-in women; when the evaluation result is a pathogenic bacteria group, advice which needs to be regulated and actively treated is given, and flora collection and evaluation are continuously carried out to monitor the flora state of pregnant and lying-in women;
Step two, after the pregnant and lying-in women with qualified flora detection enter a delivery room, collecting vaginal secretion of the lying-in women before a caesarean operation is performed by using the flora collecting auxiliary device, directly smearing a part of collected pregnant and lying-in women flora on operation gloves of a delivery doctor or a delivery nurse, and wearing the operation gloves smeared with the pregnant and lying-in women flora by the delivery doctor or the delivery nurse when taking out a fetus so that a newborn with the pregnant and lying-in women just separated from a mother comes into contact with the mother flora immediately, and particularly, the newborn needs to pay attention to directly suck part of the pregnant and lying-in women flora on the operation gloves into the intestines of the newborn through the oral cavity; and the other part of the acquired maternal bacterial flora is placed in a culture dish, so that the bacterial flora is fully obtained by sterile cotton and medical gloves, when the neonate is successfully delivered and taken out and is handed to a nurse for treating placenta and umbilical cord, the nurse can operate by the medical gloves, and when the neonate is weighed and wrapped, the skin is smeared by the sterile cotton with the obtained maternal bacterial flora and the nipple of the mother is smeared at the same time, so that the bacterial flora loss of the neonate without birth canal during delivery is reduced to the maximum.
Collecting neonatal stool by using the flora collection auxiliary device, performing fecal flora high-throughput sequencing, namely collecting neonatal stool flora, performing flora evaluation on the collected neonatal stool flora by using the maternal and infant flora transmission monitoring system, giving a suggestion that re-smearing is not needed and the neonatal stool is kept continuously when the evaluation result is a healthy flora, and continuously collecting and evaluating the flora to monitor the neonatal flora state; when the evaluation result is a missing flora, providing advice that re-smearing or probiotic supplementation and field planting are needed, and continuously collecting and evaluating flora after adjustment to monitor the neonatal flora state; and when the evaluation result is a pathogenic bacteria group, advice that probiotic bacteria are required to be regulated and actively treated is given, and flora collection and evaluation are continuously carried out to monitor the neonatal flora state.
Preferably, during the collection of the parturient flora in the second step, the parturient flora is left in a culture dish and fermented, so that the parturient flora can be used enough for smearing with neonatal fetal fecal flora, and can also be used for detecting related flora.
Compared with the prior art, the invention has the advantages that:
1. The maternal and infant flora transfer monitoring system and method disclosed by the invention are used for carrying out standardized management on the transfer of maternal and infant flora, and can be used for effectively tracking and managing intestinal flora of pregnant and lying-in women and newborns, so that important data support is provided for individual nutrition advice and health management.
2. According to the maternal and infant flora transmission monitoring system and method, health monitoring management is conducted on intestinal flora of pregnant and lying-in women, unhealthy flora structures and harmful pathogenic diseases are improved and adjusted conveniently through dietary advice, flora adjustment, even treatment and other methods, so that flora obtained from a mother body is healthier when a neonate is born, the defect of transmission of maternal thalli of the neonate after caesarean section can be timely overcome, and positive effects are played on reducing risks of diseases such as allergy, asthma and the like of the neonate.
3. The flora collection auxiliary device can safely take out secretion of a parturient birth canal before parturient birth, and can be smeared timely after parturient birth, so that the flora is contacted with a newborn birth, the colonization from a parent flora is performed timely at the key moment of setting the first intestinal flora of the newborn birth, and the flora is designed into an operation package, so that the flora of the parturient birth canal of the mother is smeared on the oral cavity and the skin of the newborn birth, and the defect of flora transmission is reduced.
Drawings
FIG. 1 is a block diagram of a system and method for monitoring the transfer of a mother-infant flora;
FIG. 2 is a flow chart of a system and method for monitoring the transfer of a mother and infant flora.
Detailed Description
In order that the invention may be readily understood, a more particular description thereof will be rendered by reference to specific embodiments that are illustrated in the appended drawings.
A maternal and infant flora transfer monitoring system comprises a maternal and parturient flora monitoring and evaluating subsystem, a neonatal flora monitoring and evaluating subsystem and a flora database,
The maternal and parturient flora monitoring and evaluating subsystem and the neonatal flora monitoring and evaluating subsystem both comprise a flora acquisition module and a flora analysis module;
The flora database is preset with flora types classified according to the maternal and infant flora transfer health grade.
Just like the constantly iterative genetic studies, the flora patterns are constantly updated according to the constantly evolving maternal and infant microbiology and immunology studies in the gestation and neonatal period. The invention takes microbiology and immunology as main dimensions, and the flora types are evaluated and classified according to the related latest researches, wherein the flora types comprise but are not limited to the following groups: a healthy group, a deleted group, and a pathogenic group;
The healthy flora comprises at least bacteroides, bifidobacteria and escherichia; the bifidobacterium genus at least comprises bifidobacterium longum and bifidobacterium breve, and the escherichia genus at least comprises escherichia coli;
if the bacterium does not contain bacteroides, the bacterium is the deletion group;
if enterobacter cloacae or klebsiella pneumoniae exists, the bacterium group is the pathogenic bacterium group;
The maternal and parturient flora monitoring and evaluating subsystem evaluates the flora according to the maternal and parturient flora acquired by the flora acquisition module, and the flora analysis module evaluates the flora in the flora database to obtain a flora type evaluation result of the maternal and parturient flora and gives related advice, and when the evaluation result is a healthy flora, advice which is continuously maintained and can be used for supplementing the neonatal flora is given; when the evaluation result is a missing flora, a suggestion that flora colonization supplementation is needed is given; when the evaluation result is a pathogenic bacteria group, advice for positive treatment and regulation is given;
The system comprises a flora analysis module, a flora database, a flora analysis module, a community type evaluation module and a display module, wherein the flora analysis module is used for performing flora evaluation according to the initial flora of the neonate, which is acquired by the flora acquisition module, to obtain a flora type evaluation result of the initial flora of the neonate and give related advice, and finally the evaluation result and the related advice are displayed on the display module; when the evaluation result is a healthy flora, advice that flora supplementation is not needed is given; when the evaluation result is the deficiency flora, advice for flora supplementation is given; and when the evaluation result is a pathogenic bacteria group, advice that the flora is required to be supplemented and actively treated is given.
Example 2
The difference between the embodiment and the above embodiment is that the maternal and infant flora transfer monitoring system further comprises a maternal milk flora monitoring and evaluating subsystem, the maternal milk flora monitoring and evaluating subsystem comprises a maternal milk flora acquisition module and a maternal milk flora analysis module, and the maternal milk flora monitoring and evaluating subsystem obtains a flora type evaluation result of the maternal milk flora and gives related advice according to the maternal milk flora acquired by the maternal milk flora acquisition module and flora type in a flora database; when the evaluation result is a healthy bacterial group, giving a suggestion for continuing to keep; when the evaluation result is a deficiency group, advice which needs to be supplemented and regulated is given; and when the evaluation result is a pathogenic bacteria group, advice which needs to be regulated and actively treated is given. Preferably, the neonatal initial flora comprises a neonatal period gestational flora.
Preferably, the maternal flora comprises flora obtained by performing high-throughput sequencing on vaginal secretion of the maternal flora.
Preferably, the maternal breast milk flora includes flora obtained by high-throughput sequencing of the maternal colostrum.
Preferably, the maternal and infant flora transfer monitoring system further comprises a display module for displaying the evaluation result and the related advice.
Example 3
The flora collection auxiliary device for assisting the maternal and infant flora transfer monitoring system in flora collection is characterized by comprising an aseptic cleaning wet tissue for cleaning the periphery of a vagina, an aseptic vaginal dilation clamp for draining vaginal secretions of a puerpera, medical aseptic cotton for dipping the vaginal secretions, aseptic medical gloves, a culture dish for culturing flora, a soft cotton stick capable of extending into the oral cavity of a neonate and soft cotton gauze capable of being smeared on the neonate. The sterile medical glove is convenient for a surgeon or nurse to wear so as to obtain the flora of the pregnant and lying-in women in a sterile environment.
The flora collection auxiliary device further comprises a heater for keeping the sterile cleaning wet tissues, the sterile vaginal dilation clips, the medical sterile cotton and the like at the body temperature; and the auxiliary bag for producing is used for sealing and placing the sterile cleaning wet tissues, the sterile vaginal dilation clamp, medical sterile cotton, a culture dish, a soft cotton stick, soft cotton gauze and a heater.
The flora collection auxiliary device also comprises an auxiliary bag to be produced, and is used for placing the sterile cleaning wet tissue, the sterile vaginal dilation clamp, medical sterile cotton, a culture dish, a soft cotton stick, soft cotton gauze and a heater.
Example 4
The maternal and infant flora transfer monitoring method is characterized by comprising the following steps of:
Collecting vaginal flora of pregnant and lying-in women by using the flora collecting auxiliary device, namely collecting the flora of the pregnant and lying-in women, carrying out flora evaluation on the collected intestinal flora of the pregnant and lying-in women by using the maternal and infant flora transmission monitoring system, giving out a suggestion for continuous maintenance when the evaluation result is a healthy flora, and continuously collecting and evaluating the flora to monitor the flora state of the pregnant and lying-in women; when the evaluation result is a deficiency flora, giving a suggestion that modification and adjustment are needed, and continuously collecting and evaluating flora after modification and adjustment to monitor the flora state of pregnant and lying-in women; when the evaluation result is a pathogenic bacteria group, giving a suggestion that modification and adjustment are needed and active treatment is carried out, and continuously collecting and evaluating flora to monitor the flora state of pregnant and lying-in women;
Step two, after the pregnant and lying-in women with qualified flora detection enter a delivery room, collecting vaginal flora of the lying-in women before a caesarean operation is performed by using the flora collecting auxiliary device, directly smearing a part of collected vaginal flora of the pregnant and lying-in women with operation gloves of a delivery doctor or a delivery nurse, and wearing the operation gloves smeared with the maternal flora by the delivery doctor or the delivery nurse when taking out a fetus so that a newborn with the pregnant and lying-in women just separated from a mother can be immediately contacted with the maternal flora, and particularly, the newborn needs to pay attention to directly suck part of the maternal flora on the operation gloves into the intestines of the newborn through the oral cavity; and the other part of the acquired maternal bacterial flora is placed in a culture dish, so that the bacterial flora is fully obtained by sterile cotton and medical gloves, when the neonate is successfully delivered and taken out and is handed to a nurse for treating placenta and umbilical cord, the nurse can operate by the medical gloves, and when the neonate is weighed and wrapped, the skin is smeared by the sterile cotton with the obtained maternal bacterial flora and the nipple of the mother is smeared at the same time, so that the bacterial flora loss of the neonate without birth canal during delivery is reduced to the maximum.
Collecting neonatal stool by using the flora collection auxiliary device, performing fecal flora high-throughput sequencing, namely collecting neonatal stool flora, performing flora evaluation on the collected neonatal stool flora by using the maternal and infant flora transmission monitoring system, giving a suggestion that re-smearing is not needed and the neonatal stool is kept continuously when the evaluation result is a healthy flora, and continuously collecting and evaluating the flora to monitor the neonatal flora state; when the evaluation result is a missing flora, providing advice that re-smearing or probiotic supplementation and field planting are needed, and continuously collecting and evaluating flora after adjustment to monitor the neonatal flora state; and when the evaluation result is a pathogenic bacteria group, advice that probiotic bacteria are required to be regulated and actively treated is given, and flora collection and evaluation are continuously carried out to monitor the neonatal flora state.
Preferably, in the second step, the maternal bacterial flora is collected, and the maternal bacterial flora is left in a culture dish and fermented, so that the maternal bacterial flora can be used enough when being smeared with newborns, and the maternal bacterial flora detection method can also be applied to bacterial flora detection.
While embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are illustrative and not to be construed as limiting the invention, and that variations, modifications, alternatives and variations may be made to the above embodiments by one of ordinary skill in the art within the scope of the invention.
Claims (8)
1. The maternal and infant flora transfer monitoring system is characterized by comprising a maternal and puerperal flora monitoring and evaluating subsystem, a neonate flora monitoring and evaluating subsystem, a flora database, a display module and a storage module;
the maternal and parturient flora monitoring and evaluating subsystem comprises a flora acquisition module and a flora analysis module;
The flora database is preset with flora types classified according to maternal and infant flora transfer health grades, and the flora types can be updated in time and iteratively according to the continuously developed maternal and infant microbiology and immunology researches in the gestation period and the neonatal period; the types of flora currently used for pregnant and lying-in women include, but are not limited to: a healthy group of bacteria, a deleted group of bacteria and a pathogenic group of bacteria;
The healthy flora comprises at least bacteroides, bifidobacteria and escherichia; the bifidobacterium genus at least comprises bifidobacterium longum and bifidobacterium breve, and the escherichia genus at least comprises escherichia coli;
if the bacterium does not contain bacteroides, the bacterium is the deletion group;
if enterobacter cloacae or klebsiella pneumoniae exists, the bacterium group is the pathogenic bacterium group;
The maternal and parturient flora monitoring and evaluating subsystem evaluates the flora according to the maternal and parturient flora acquired by the flora acquisition module, and the flora analysis module evaluates the flora in the flora database to obtain a flora type evaluation result of the maternal and parturient flora and gives related advice, and when the evaluation result is a healthy flora, advice which is continuously maintained and can be used for supplementing the neonatal flora is given; when the evaluation result is a missing flora, a suggestion that flora colonization supplementation is needed is given; when the evaluation result is a pathogenic bacteria group, giving a suggestion for active treatment and regulation;
The system comprises a flora analysis module, a flora database, a flora analysis module, a community type evaluation module and a display module, wherein the flora analysis module is used for performing flora evaluation according to the initial flora of the neonate, which is acquired by the flora acquisition module, to obtain a flora type evaluation result of the initial flora of the neonate and give related advice, and finally the evaluation result and the related advice are displayed on the display module; when the evaluation result is a healthy flora, advice that flora supplementation is not needed is given; when the evaluation result is the deficiency flora, advice for flora supplementation is given; when the evaluation result is a pathogenic bacteria group, giving advice for supplementing and actively treating the bacteria group, wherein the neonatal initial bacteria group comprises fetal excrement bacteria group during neonatal period;
The display module is used for displaying the evaluation result and the related advice, and the storage module is used for storing the maternal and maternal milk flora and the neonatal flora according to the setting of each user.
2. The maternal and infant flora transfer monitoring system according to claim 1, further comprising a maternal milk flora monitoring and evaluation subsystem comprising a maternal milk flora acquisition module and a maternal milk flora analysis module, wherein the maternal milk flora monitoring and evaluation subsystem obtains a flora type evaluation result of the maternal milk flora and gives related advice by performing flora evaluation of the flora type in the maternal milk flora database according to the maternal milk flora acquired by the maternal milk flora acquisition module; when the evaluation result is a healthy bacterial group, giving a suggestion for continuing to keep; when the evaluation result is a deficiency group, advice which needs to be supplemented and regulated is given; and when the evaluation result is a pathogenic bacteria group, advice which needs to be regulated and actively treated is given.
3. The maternal and infant flora transfer monitoring system of claim 2, wherein said maternal breast milk flora comprises a flora obtained by high throughput sequencing of the flora of maternal colostrum.
4. The maternal and infant flora transfer monitoring system of claim 1, wherein said maternal flora comprises a flora obtained after high throughput sequencing of its vaginal secretions.
5. A flora collection assisting device for assisting the flora collection of a maternal and infant flora transfer monitoring system according to any one of claims 1-4, characterized by comprising a sterile cleaning wet tissue for cleaning the periphery of the vagina, a sterile vaginal dilation clip for draining vaginal secretions of a puerpera, medical sterile cotton for dipping the vaginal secretions, medical gloves, a culture dish for culturing the flora, a soft cotton stick capable of being stretched into the oral cavity of a neonate, and soft cotton gauze capable of being smeared on the neonate and breasts of a puerpera.
6. The flora collection attachment of claim 5, further comprising a heater for maintaining the sterile cleaning wet wipe, sterile vaginal dilation clip, medical sterile cotton at body temperature; and the auxiliary bag for producing is used for sealing and placing the sterile cleaning wet tissues, the sterile vaginal dilation clamp, medical sterile cotton, a culture dish, a soft cotton stick, soft cotton gauze and a heater.
7. The maternal and infant flora transfer monitoring method is characterized by comprising the following steps of:
Collecting the flora of the pregnant and lying-in women by using the flora collection auxiliary device according to claim 5 or 6, namely collecting the flora of the pregnant and lying-in women, performing flora evaluation on the collected intestinal flora of the pregnant and lying-in women by using the maternal and infant flora transmission monitoring system according to any one of claims 1-4, giving a suggestion for continuous maintenance when the evaluation result is a healthy flora, and continuously collecting and evaluating the flora to monitor the flora state of the pregnant and lying-in women; when the evaluation result is a deficiency flora, giving a suggestion to be regulated, and continuously collecting and evaluating flora after regulation to monitor the flora state of pregnant and lying-in women; when the evaluation result is a pathogenic bacteria group, advice which needs to be regulated and actively treated is given, and flora collection and evaluation are continuously carried out to monitor the flora state of pregnant and lying-in women;
Step two, after the pregnant and lying-in women with qualified flora detection enter a delivery room, collecting vaginal flora of the lying-in women before a caesarean operation is performed by using the flora collecting auxiliary device, directly smearing a part of collected vaginal flora of the pregnant and lying-in women with operation gloves of a delivery doctor or a delivery nurse, and wearing the operation gloves smeared with the maternal flora by the delivery doctor or the delivery nurse when taking out a fetus so that a newborn with the pregnant and lying-in women just separated from a mother can be immediately contacted with the maternal flora, and particularly, the newborn needs to pay attention to directly suck part of the maternal flora on the operation gloves into the intestines of the newborn through the oral cavity; placing the other part of acquired maternal and parturient flora into a culture dish, fully obtaining the flora by sterile cotton and medical gloves, and operating by the medical gloves when the neonate is delivered to a nurse to treat placenta and umbilical cord after successful delivery, and smearing the skin by the sterile cotton with the obtained maternal flora when the neonate is weighed up and wrapped, and smearing the nipple of the mother at the same time so as to maximally reduce the flora deficiency of the neonate without birth canal during delivery;
Collecting neonatal stool by using the flora collection auxiliary device, performing fecal flora high-throughput sequencing, namely collecting neonatal stool flora, performing flora evaluation on the collected neonatal stool flora by using the maternal and infant flora transmission monitoring system, giving a suggestion that re-smearing is not needed and the neonatal stool is kept continuously when the evaluation result is a healthy flora, and continuously collecting and evaluating the flora to monitor the neonatal flora state; when the evaluation result is a missing flora, providing advice that re-smearing or probiotic supplementation and field planting are needed, and continuously collecting and evaluating flora after adjustment to monitor the neonatal flora state; and when the evaluation result is a pathogenic bacteria group, advice that probiotic bacteria are required to be regulated and actively treated is given, and flora collection and evaluation are continuously carried out to monitor the neonatal flora state.
8. The method according to claim 7, wherein during the collection of the maternal bacterial flora in the second step, the maternal bacterial flora is left in a culture dish and fermented, so that the maternal bacterial flora can be used enough for application with neonatal fetal fecal bacterial flora, and can also be used for bacterial flora detection.
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2026681C1 (en) * | 1991-06-18 | 1995-01-20 | Оренбургский Государственный Медицинский Институт | Method of forming microflora of neonate's intestine in prenatal preparation of woman in childbirth |
| CN102462538A (en) * | 2010-11-10 | 2012-05-23 | 程定超 | Disposable gynecological medical appliance bag |
| CN105288605A (en) * | 2015-11-13 | 2016-02-03 | 刘传新 | First bacterination method for caesarean delivery babies |
| WO2016183535A1 (en) * | 2015-05-14 | 2016-11-17 | University Of Puerto Rico | Methods for restoring microbiota of newborns |
| CN106267383A (en) * | 2016-09-22 | 2017-01-04 | 南昌大学 | A kind of preparation method for recovering cesarean neonate symbiotic microorganism rag |
| CN106529128A (en) * | 2016-10-21 | 2017-03-22 | 大连千松科技有限公司 | Internet-based postpartum health assessment method and apparatus |
| CN207259507U (en) * | 2017-08-29 | 2018-04-20 | 李同民 | A kind of gynaecology's private parts flora samples classification inoculation apparatus |
| CN108841974A (en) * | 2018-06-28 | 2018-11-20 | 北京水母科技有限公司 | Extract the method that fecal sample 16s rRNA is monitored in infant's intestinal microecology maturity |
| CN110060778A (en) * | 2019-04-23 | 2019-07-26 | 完美(上海)健康科技有限公司 | Using intestinal flora as the health management scheme of target spot |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105796606A (en) * | 2014-12-29 | 2016-07-27 | 广州海思医疗科技有限公司 | Probiotics composition for reducing risk of fetal congenital heart disease and detecting method thereof |
| CN111445957B (en) * | 2020-02-25 | 2024-05-24 | 北京德润良品健康科技有限公司 | Mother and infant flora transfer monitoring system and method |
-
2020
- 2020-02-25 CN CN202010117604.XA patent/CN111445957B/en active Active
-
2021
- 2021-02-25 WO PCT/CN2021/077780 patent/WO2021170019A1/en active Application Filing
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2026681C1 (en) * | 1991-06-18 | 1995-01-20 | Оренбургский Государственный Медицинский Институт | Method of forming microflora of neonate's intestine in prenatal preparation of woman in childbirth |
| CN102462538A (en) * | 2010-11-10 | 2012-05-23 | 程定超 | Disposable gynecological medical appliance bag |
| WO2016183535A1 (en) * | 2015-05-14 | 2016-11-17 | University Of Puerto Rico | Methods for restoring microbiota of newborns |
| CN105288605A (en) * | 2015-11-13 | 2016-02-03 | 刘传新 | First bacterination method for caesarean delivery babies |
| CN106267383A (en) * | 2016-09-22 | 2017-01-04 | 南昌大学 | A kind of preparation method for recovering cesarean neonate symbiotic microorganism rag |
| CN106529128A (en) * | 2016-10-21 | 2017-03-22 | 大连千松科技有限公司 | Internet-based postpartum health assessment method and apparatus |
| CN207259507U (en) * | 2017-08-29 | 2018-04-20 | 李同民 | A kind of gynaecology's private parts flora samples classification inoculation apparatus |
| CN108841974A (en) * | 2018-06-28 | 2018-11-20 | 北京水母科技有限公司 | Extract the method that fecal sample 16s rRNA is monitored in infant's intestinal microecology maturity |
| CN110060778A (en) * | 2019-04-23 | 2019-07-26 | 完美(上海)健康科技有限公司 | Using intestinal flora as the health management scheme of target spot |
Non-Patent Citations (2)
| Title |
|---|
| 母婴间菌群传递的研究进展;边燕飞;孙志宏;孙天松;;中国微生态学杂志(第06期);第725-728页 * |
| 边燕飞 ; 孙志宏 ; 孙天松 ; .母婴间菌群传递的研究进展.中国微生态学杂志.2017,(第06期),第725-728页. * |
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