CN111443193A - Synthesize control by temperature change incubator - Google Patents
Synthesize control by temperature change incubator Download PDFInfo
- Publication number
- CN111443193A CN111443193A CN202010217037.5A CN202010217037A CN111443193A CN 111443193 A CN111443193 A CN 111443193A CN 202010217037 A CN202010217037 A CN 202010217037A CN 111443193 A CN111443193 A CN 111443193A
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- heating pot
- liquid
- cover plate
- temperature
- heating
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- 238000010438 heat treatment Methods 0.000 claims abstract description 51
- 239000011521 glass Substances 0.000 claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000007788 liquid Substances 0.000 claims description 56
- 238000002474 experimental method Methods 0.000 claims description 8
- 238000001514 detection method Methods 0.000 claims description 3
- 238000009434 installation Methods 0.000 claims description 3
- 239000002699 waste material Substances 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 4
- 238000004321 preservation Methods 0.000 abstract description 3
- 238000011534 incubation Methods 0.000 description 5
- 230000002055 immunohistochemical effect Effects 0.000 description 4
- 238000007599 discharging Methods 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 238000005067 remediation Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a comprehensive temperature control incubator, which comprises a supporting plate (7), a constant temperature water bath box (2), a slide bracket (3), a cover plate (8), a heating pot (10) and a sliding guide rail (4); the heating pot (10) is embedded into the constant-temperature water bath box (2), a plurality of glass slide supports (3) are placed in the heating pot (10), a plurality of cover plate grooves are formed in the glass slide supports (3), 1 cover plate (8) is buckled on each cover plate groove, and 1 glass slide (9) is attached to the inner surface of each cover plate (8); 2 sliding guide rails (4) are respectively fixed on the inner sides of the two supporting plates (7), the heating plate (14) is fixed at the bottom of the heating pot (10), 2 guide blocks are respectively fixed on two side faces of the constant-temperature water bath box (2), and the 2 guide blocks are respectively in sliding connection with the sliding guide rails (4). The invention realizes carrying of a plurality of glass slides at one time, and has the advantages of high efficiency, low cost, good temperature control effect and balanced and reliable moisture preservation.
Description
Technical Field
The invention relates to the technical field of biological sample treatment, in particular to a comprehensive temperature control incubator.
Background
Currently, the automated devices associated with immunohistochemical experiments have some disadvantages, such as: the number of samples processed at one time is too small; a separate high-temperature incubation device is needed, and the structure of the high-temperature incubation device is too complex; the integral temperature control precision is not high, the quality of the glass slide is affected, the integral harmony is not good, and the cost is relatively high.
Disclosure of Invention
The invention aims to provide a comprehensive temperature control incubator, which realizes low cost, high efficiency, good temperature control effect, simple structure, and balanced and reliable moisture preservation, and is suitable for immunohistochemical experiment incubation.
The technical scheme of the invention is that the comprehensive temperature control incubator is characterized in that: the incubator comprises a supporting plate, a constant-temperature water bath box, a slide bracket, a cover plate, a slide glass, a heating pot, a sliding guide rail and a heating plate; the heating pot is embedded in the constant-temperature water bath box and filled with liquid, the plurality of slide supports are transversely and parallelly placed in the heating pot along the heating pot, a plurality of cover plate grooves are formed in the slide supports and are arranged one by one along the length direction of the slide supports, 1 cover plate is buckled on each cover plate groove, and 1 glass slide is attached to the inner surface of each cover plate; 2 sliding guide fixes respectively at two backup pads inboardly, and 2 backup pads are located the both sides of constant temperature water bath respectively, and the bottom at the heating pot is fixed to the hot plate, and 2 guide blocks are fixed respectively on the both sides face of constant temperature water bath, 2 guide blocks respectively with sliding guide sliding connection, and 2 backup pads are fixed respectively on the bottom plate, and the bottom plate is fixed on experiment platform.
The incubator also comprises a liquid inlet pipeline and a liquid discharge pipeline; one end of the liquid inlet pipeline is communicated with a liquid purifying box in the experimental equipment, and the other end of the liquid inlet pipeline is communicated with the heating pot through a pipeline connecting piece; one end of the liquid discharge pipeline is communicated with the heating pot through a pipeline connecting piece, and the other end of the liquid discharge pipeline is communicated with a waste liquid tank in the experimental equipment.
The liquid inlet pipeline and the liquid outlet pipeline adopt flexible pipes, and elastic hose clamp hoops are arranged at the joints of the pipeline connecting pieces to fix the flexible pipes.
The incubator further comprises a temperature sensor; the temperature sensor is fixedly arranged on the inner side of the heating pot, the installation position of the temperature sensor is required to be below the liquid level, and the temperature sensor is used for detecting the real-time temperature of liquid in the heating pot and outputting the detection value to a computer in the experimental equipment.
The liquid in the heating pan is added to the heating pan volume at 2/3.
The sliding guide rail adopts three sections of silent undamped guide rails.
A maximum of 16 cover plates are placed in each slide rack.
A maximum of 3 slide holders are placed in the heating pan.
The integrated glass slide incubator has the advantages that the integrated structure is adopted, the purposes of loading and high-temperature incubation of glass slides are achieved, and the structure is simple; according to the invention, the carrying amount of the glass slides is increased, so that 48 glass slides can be carried at one time, and the glass slide carrying structure is integrated and has higher stability; the structure of the invention adopts time control liquid adding and discharging to replace the traditional liquid level detection sensing, on one hand, the design is simplified, and on the other hand, the effect of controlling the liquid adding and discharging is more accurate; the integral structure of the invention has the advantages of high efficiency, low cost, good temperature control effect and balanced and reliable moisture preservation, and is suitable for an immunohistochemical experiment incubation system.
Drawings
FIG. 1 is a schematic structural diagram of an integrated temperature control incubator of the present invention.
Fig. 2 is a top view of fig. 1.
Detailed Description
The technical scheme of the invention is described in detail in the following with the accompanying drawings and specific embodiments of the specification.
As shown in fig. 1 and fig. 2, the integrated temperature control incubator of the present invention comprises a liquid inlet pipe 1, a liquid outlet pipe 5, a bottom plate 6, a temperature sensor 13, a support shaft 11, a support plate 7, a constant temperature water bath 2, a slide support 3, a cover plate 8, a slide glass 9, a heating pan 10, a slide rail 4, a pipe connection member 12, and a heating plate 14; the line connection 12 employs an M8 male adapter. The heating pan 10 is embedded in the constant temperature water bath tank 2. The plurality of glass slide supports 3 are horizontally and parallelly arranged in the heating pot 10 along the heating pot 10, a plurality of cover plate grooves are arranged in the glass slide supports 3, and the cover plate grooves are arranged one by one along the length direction of the glass slide supports 3, namely, are arranged along the longitudinal direction of the heating pot 10. Each cover plate groove is buckled with 1 cover plate 8. A plurality of slides 9 are respectively attached to the inner surfaces of the plurality of cover plates 8. 2 sliding guide 4 are respectively fixed mounting at two backup pads 7 inboards, and 2 backup pads 7 are located the both sides of thermostatted water bath 2 respectively. The heating plate 14 is fixedly installed at the bottom of the heating pot 10 through screws, 2 guide blocks are respectively fixedly installed on two side surfaces of the constant temperature water bath box 2 through screws, and the 2 guide blocks are respectively in sliding connection with the sliding guide rail 4. The 2 supporting plates 7 are respectively fixedly arranged on the bottom plate 6 through 1 supporting shaft 11. The liquid inlet pipeline 1 and the liquid discharge pipeline 5 are respectively connected with the heating pot 10 through an M8 external thread adapter 12, namely, one end of the liquid inlet pipeline 1 is communicated with a liquid purifying box in experimental equipment, and the other end of the liquid inlet pipeline 1 is communicated with the heating pot 10 through a pipeline connecting piece 12; one end of the liquid discharge pipeline 5 is communicated with the heating pot 10 through a pipeline connecting piece, and the other end of the liquid discharge pipeline 5 is communicated with a waste liquid tank in the experimental equipment. The temperature sensor 13 is fixedly arranged on the inner side of the heating pot 10, and the bottom plate 6 is fixedly arranged on an experiment platform through screws. The heating pan 10 is filled with liquid, i.e. the constant temperature water bath tank 2 is seated in the liquid. The liquid in the heating pot 10 adopts water or repair liquid.
In practical application, the liquid inlet pipeline 1 and the liquid outlet pipeline 5 adopt flexible pipes. The liquid inlet pipeline 1 and the liquid discharge pipeline 5 are controlled by a main control console of the immunohistochemical experiment system to feed liquid and discharge liquid, and elastic hose hooping water pipe clamps are arranged at the joints of the pipeline connecting pieces 12 to fix the hoses.
In practice, water or remediation fluid is added to the heating kettle 10 at 2/3 of its volume.
In practical application, the temperature sensor 13 is used for detecting the real-time temperature of the liquid in the heating pan 10 and outputting the detected value to a computer in the experimental facility, and the installation position of the temperature sensor 13 must be below the liquid level.
In practical applications, the heating pan 10 is used for heating liquid, and sealing measures are provided at all pipe joints.
In practical application, the sliding guide rail 4 is a three-section silent undamped guide rail, and is installed and fixed on the inner side of the support plate 7.
In practice, a maximum of 16 cover plates 8 are placed in each slide rack 3 per slide rack. A maximum of 16 cover plates 8 are placed in 3. The plurality of slide racks 3 is loaded with a maximum of 48 cover plates at a time.
In practice, only one slide 9 per cover plate 8 can be placed.
In practice, the M8 male adapter 12 of the drain line 5 is mounted in a hole flush with the inside bottom surface of the heating pan 10.
In practical application, the glass slide 9 is attached to the cover plate 8 in a self-adaptive manner and needs to be pressed forcibly.
To sum up, this structure increases the disposable volume of experiment achievement, can realize once only carrying 48 slides, and the comprehensive control by temperature change incubator of this structure adopts time control liquid feeding and flowing back to replace traditional liquid level to detect the sensing, makes it more accurate, has high efficiency, low cost, the effectual and balanced reliable advantage of moisturizing of control by temperature change.
The foregoing has described the general principles, essential features, and advantages of the invention. It should be understood by those skilled in the art that the foregoing embodiments are merely illustrative of the technical spirit and features of the present invention, and the present invention is not limited thereto but may be implemented by those skilled in the art.
Claims (8)
1. The utility model provides a synthesize control by temperature change incubator which characterized by: the incubator comprises a supporting plate (7), a constant temperature water bath box (2), a slide glass bracket (3), a cover plate (8), a slide glass (9), a heating pot (10), a sliding guide rail (4) and a heating plate (14); the heating pot (10) is embedded in the constant-temperature water bath box (2), liquid is filled in the heating pot (10), the plurality of glass slide supports (3) are transversely placed in the heating pot (10) in parallel along the heating pot (10), a plurality of cover plate grooves are formed in the glass slide supports (3), the plurality of cover plate grooves are arranged one by one along the length direction of the glass slide supports (3), 1 cover plate (8) is buckled on each cover plate groove, and 1 glass slide (9) is attached to the inner surface of each cover plate (8); 2 sliding guide (4) are fixed respectively at two backup pads (7) inboards, 2 backup pads (7) are located the both sides of constant temperature water bath case (2) respectively, the bottom at heating pot (10) is fixed in hot plate (14), 2 guide blocks are fixed respectively on the both sides face of constant temperature water bath case (2), 2 guide blocks respectively with sliding guide (4) sliding connection, 2 backup pads (7) are fixed respectively on bottom plate (6), bottom plate (6) are fixed on the experiment platform.
2. A comprehensive temperature-controlled incubator according to claim 1, wherein: the incubator also comprises a liquid inlet pipeline (1) and a liquid outlet pipeline (5); one end of the liquid inlet pipeline (1) is communicated with a liquid purifying tank in the experimental equipment, and the other end of the liquid inlet pipeline (1) is communicated with the heating pot (10) through a pipeline connecting piece (12); one end of the liquid discharge pipeline (5) is communicated with the heating pot (10) through a pipeline connecting piece, and the other end of the liquid discharge pipeline (5) is communicated with a waste liquid tank in the experimental equipment.
3. A comprehensive temperature-controlled incubator according to claim 2, wherein: the liquid inlet pipeline (1) and the liquid outlet pipeline (5) adopt flexible pipes, and elastic hose hoops are arranged at the joints of the pipeline connecting pieces (12) to clamp the water pipes to fix the flexible pipes.
4. A comprehensive temperature-controlled incubator according to claim 1, wherein: the incubator further comprises a temperature sensor (13); the temperature sensor (13) is fixedly arranged at the inner side of the heating pot (10), the installation position of the temperature sensor (13) is required to be below the liquid level, and the temperature sensor (13) is used for detecting the real-time temperature of the liquid in the heating pot (10) and outputting the detection value to a computer in experimental equipment.
5. A comprehensive temperature-controlled incubator according to claim 1, wherein: the liquid in the heating pan (10) is added to the heating pan (10) at 2/3 of the volume.
6. A comprehensive temperature-controlled incubator according to claim 1, wherein: the sliding guide rail (4) adopts three sections of silent undamped guide rails.
7. A comprehensive temperature-controlled incubator according to claim 1, wherein: a maximum of 16 cover plates (8) are placed in each slide holder (3).
8. A comprehensive temperature-controlled incubator according to claim 1, wherein: and at most 3 slide supports (3) are placed in the heating pot (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010217037.5A CN111443193A (en) | 2020-03-25 | 2020-03-25 | Synthesize control by temperature change incubator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010217037.5A CN111443193A (en) | 2020-03-25 | 2020-03-25 | Synthesize control by temperature change incubator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111443193A true CN111443193A (en) | 2020-07-24 |
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ID=71629565
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010217037.5A Pending CN111443193A (en) | 2020-03-25 | 2020-03-25 | Synthesize control by temperature change incubator |
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| Country | Link |
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| CN (1) | CN111443193A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112816683A (en) * | 2020-12-28 | 2021-05-18 | 广州金域医学检验中心有限公司 | Incubator for slide incubation and method of use |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106226150A (en) * | 2016-08-31 | 2016-12-14 | 盐城拜明生物技术有限公司 | Concussion incubating device |
| CN106932601A (en) * | 2017-05-02 | 2017-07-07 | 深圳市活水床旁诊断仪器有限公司 | A kind of Biochemical Analyzer and its detection method |
| CN207730528U (en) * | 2018-02-05 | 2018-08-14 | 中南大学湘雅二医院 | A kind of novel immune groupization experiment incubating device |
| CN211978936U (en) * | 2020-03-25 | 2020-11-20 | 北京海格莱生物科技有限公司 | Synthesize control by temperature change incubator |
-
2020
- 2020-03-25 CN CN202010217037.5A patent/CN111443193A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106226150A (en) * | 2016-08-31 | 2016-12-14 | 盐城拜明生物技术有限公司 | Concussion incubating device |
| CN106932601A (en) * | 2017-05-02 | 2017-07-07 | 深圳市活水床旁诊断仪器有限公司 | A kind of Biochemical Analyzer and its detection method |
| CN207730528U (en) * | 2018-02-05 | 2018-08-14 | 中南大学湘雅二医院 | A kind of novel immune groupization experiment incubating device |
| CN211978936U (en) * | 2020-03-25 | 2020-11-20 | 北京海格莱生物科技有限公司 | Synthesize control by temperature change incubator |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112816683A (en) * | 2020-12-28 | 2021-05-18 | 广州金域医学检验中心有限公司 | Incubator for slide incubation and method of use |
| CN112816683B (en) * | 2020-12-28 | 2024-06-11 | 广州金域医学检验中心有限公司 | Incubator for slide incubation and method of use thereof |
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