CN111440717A - Dynamic mechanical loading device based on orifice plate - Google Patents
Dynamic mechanical loading device based on orifice plate Download PDFInfo
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- CN111440717A CN111440717A CN202010337928.4A CN202010337928A CN111440717A CN 111440717 A CN111440717 A CN 111440717A CN 202010337928 A CN202010337928 A CN 202010337928A CN 111440717 A CN111440717 A CN 111440717A
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- plate
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- loading device
- dynamic mechanical
- mechanical loading
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
Abstract
The invention discloses a dynamic mechanical loading device based on a pore plate, which comprises a supporting plate, the pore plate arranged on the supporting plate, a plurality of primary pressure plates, a secondary pressure plate arranged above the primary pressure plate, a resetting piece arranged between the secondary pressure plate and the supporting plate and a driving unit driving the secondary pressure plate to move towards the primary pressure plate; be provided with a plurality of holding chambeies on the orifice plate, the holding intracavity is provided with fixed module, is provided with on the fixed module and cultivates the chamber, one-level clamp plate and cultivates chamber looks adaptation. The drive unit comprises a driving piece, a connecting piece and a drive pressing plate arranged on the connecting piece, the drive pressing plate is arranged on the upper side of the pressing plate, and a mechanical sensor is further arranged between the drive pressing plate and the connecting piece. The invention can realize the dynamic pressure application to the biological materials and cells in different holes in the pore plate at the same time, thereby constructing the biomechanical conditions of the cells.
Description
Technical Field
The invention relates to the technical field of cell engineering and tissue engineering, in particular to a dynamic mechanical loading device based on a pore plate.
Background
The traditional tissue engineering construction method is to inoculate cells on a three-dimensional scaffold material in vitro, and then implant the cells into the body for repair after a period of culture. Cells, growth factors and materials are three major elements of tissue engineering, and the regulation of biological behaviors of cells and surrounding environment plays a crucial role in the culture of cells and tissues.
By changing the hydrodynamic environment generated in the scaffold material, the formation of dynamic culture has become a new trend of tissue engineering cell in vitro culture. Therefore, it is important to design a dynamic mechanical loading bioreactor to simulate the mechanical environment in vivo.
Disclosure of Invention
The purpose of the invention is: the dynamic mechanical loading device based on the pore plate can easily realize simultaneous dynamic pressure application on biological materials and cells in different pores in the pore plate to construct cell biomechanical conditions.
The purpose of the invention is realized by the following technical scheme: the device comprises a supporting plate, a pore plate arranged on the supporting plate, a plurality of primary pressure plates, a secondary pressure plate arranged above the primary pressure plate, a resetting piece arranged between the secondary pressure plate and the supporting plate and a driving unit driving the secondary pressure plate to move towards the primary pressure plate;
the pore plate is provided with a plurality of accommodating cavities, fixing modules are arranged in the accommodating cavities, culture cavities are arranged on the fixing modules, and the primary pressing plate is matched with the culture cavities;
the driving unit comprises a driving piece, a connecting piece and a driving pressing plate arranged on the connecting piece, the driving pressing plate is arranged on the upper side of the pressing plate, and a mechanical sensor is further arranged between the driving pressing plate and the connecting piece.
Furthermore, a plurality of sliding support rods are arranged on the support plate, and the secondary pressing plate is slidably mounted on the sliding support rods.
Furthermore, a sliding hole in clearance fit with the sliding support rod is formed in the secondary pressing plate.
Furthermore, the secondary pressing plate is a rectangular plate, and the sliding supporting rods are arranged at four corners of the secondary pressing plate.
Further, the reset piece is a spring sleeved on the outer side of the sliding support rod; the two ends of the reset piece are respectively abutted to the secondary pressing plate and the supporting plate.
Further, the dynamic mechanical loading device based on the orifice plate further comprises a bracket fixed on the supporting plate;
the driving piece is fixed on the bracket through a driving piece base, and a plurality of driving piece sliding rods extending along the longitudinal direction are arranged on the driving piece base;
the connecting piece has T type structure, including the connecting rod and with the sliding block of connecting rod perpendicular connection, the sliding block is installed on the driving piece slide bar.
Furthermore, a first limiting part and a second limiting part are further arranged on the driving part base, and the first limiting part and the second limiting part are arranged on two sides of the driving part sliding rod.
Further, the support comprises an upper support plate and a support rod, and the support rod is fixedly connected with the upper support plate and the support plate;
the driving piece base is fixed on the upper supporting plate, and a supporting sliding hole in clearance fit with the connecting rod is formed in the upper supporting plate.
Further, the primary pressure plate is of a T-shaped structure and comprises a crimping part in clearance fit with the culture cavity and a limiting part fixed on the upper side of the crimping part, and the diameter of the cross section of the limiting part is larger than that of the cross section of the crimping part.
Further, the driving piece is a linear motor, an air cylinder or an injection pump.
Compared with the prior art, the invention has the following advantages and effects: the driving part drives the driving pressing plate fixed on the connecting part to move up and down through the connecting part, the driving pressing plate is close to or far away from the pressing plate to move, when the driving pressing plate moves in the direction of the pressing plate, the pressing plate is pushed to move in the direction of the first-stage pressing plate, and finally the pressure is applied to the biological material inoculated with cells in the culture cavity of the fixing module, so that the biological material and the cells are pressurized. When the acting force of the driving piece is removed, the pressure reduction on the biological materials and cells can be realized by matching with the reset piece, so that the device can easily and dynamically apply pressure on the biological materials and cells. The dynamic mechanical loading device is reasonable in structure, and can easily perform experiment to simultaneously dynamically apply pressure to biological materials and cells in different holes in the pore plate, so that the construction of cell biomechanical conditions is realized.
Drawings
FIG. 1 is a schematic structural diagram of a dynamic mechanical loading device based on an orifice plate according to an embodiment of the present invention;
fig. 2 is an exploded view of a dynamic mechanical loading device based on an orifice plate according to an embodiment of the present invention.
Wherein, in the embodiment of the invention: 1. a support plate; 11. a sliding support bar; 2. an orifice plate; 3. a first-stage pressing plate; 31. a crimping part; 32. a limiting part; 4. a secondary pressing plate; 5. a drive unit; 51. a drive member base; 511. a first limiting part; 512. a second limiting part; 52. a connecting member; 521. a connecting rod; 522. a slider; 53. driving the platen; 54. a mechanical sensor; 55. a driver slide bar; 6. a reset member; 7. a fixed module; 71. a culture chamber; 8. a support; 81. an upper support plate; 82. a support rod.
Detailed Description
The present invention will be described in further detail with reference to examples and drawings, but the present invention is not limited thereto.
As shown in fig. 1 and 2, an embodiment of the present invention discloses a dynamic mechanical loading device based on a hole plate, which includes a supporting plate 1, a hole plate 2 placed on the supporting plate 1, a plurality of primary pressure plates 3, a secondary pressure plate 4 disposed above the primary pressure plate 3, a reset member 6 disposed between the secondary pressure plate 4 and the supporting plate 1, and a driving unit 5 for driving the secondary pressure plate 4 to move toward the primary pressure plate 3.
Reset 6 drive secondary pressure plate 4 and move to the direction of keeping away from one-level clamp plate 3, when the effort that drive unit 5 acted on secondary pressure plate 4 was greater than the effort of reset 6 to secondary pressure plate 4, drive unit 5 drive secondary pressure plate 4 and move to the direction of being close to one-level clamp plate 3, and reset 6 after the effort that drive unit 5 acted on secondary pressure plate 4 weakens then drive secondary pressure plate 4 and move to the direction of keeping away from one-level clamp plate 3. The secondary pressure plate 4 is moved closer to or away from the primary pressure plate 3 by the interaction of the reset member 6 and the drive unit 5.
The secondary pressing plate 4 is arranged above the primary pressing plate 3, and when the secondary pressing plate 4 moves towards the primary pressing plate 3, the secondary pressing plate 4 transmits the downward force transmitted by the driving unit 5 to the primary pressing plate 3.
In addition, a plurality of accommodating cavities are formed in the orifice plate 2, fixing modules 7 are arranged in the accommodating cavities, and culture cavities 71 are formed in the fixing modules 7. The culture chambers 71 are used for placing the adjusted cell suspension and biological material, and each culture chamber 71 is filled with 1ml of cell suspension in the embodiment, so as to ensure that ten thousand cells are inoculated. The biomaterial used in this example was a polyurethane/collagen composite scaffold.
In this embodiment, the primary pressure plate 3 is fitted to the culture chamber 71. The primary pressure plate 3 transmits the downward pressure received by the secondary pressure plate 4 to the biological material inoculated with cells in the culture chamber 71, thereby influencing the flow of the culture medium around the biological material in the culture chamber 71 and the stress of the cells on the material.
The driving unit 5 comprises a driving element, a connecting element 52 and a driving pressure plate 53 arranged on the connecting element 52, the driving pressure plate 53 is arranged on the upper side of the secondary pressure plate 4, and a mechanical sensor 54 is arranged between the driving pressure plate 53 and the connecting element 52. The pressure transmitted by the driving platen 53 can be detected in real time by the mechanical sensor 54, and then the driving member can be dynamically adjusted. The pressure of the driving member is transmitted to the biological material inoculated with the cells in the culture chamber 71, thereby dynamically influencing the flow of the culture medium around the biological material in the culture chamber 71 and the stress of the cells on the material.
In addition, the driving member drives the driving platen 53 fixed on the connecting member 52 to move up and down through the connecting member 52, and moves closer to or away from the secondary platen 4, and when moving in the direction of the secondary platen 4, the driving member pushes the secondary platen 4 to move in the direction of the primary platen 3, and finally applies pressure to the biomaterial of the cells inoculated in the culture chamber 71 of the fixing module 7, thereby realizing pressurization of the biomaterial and the cells. After the acting force of the driving part is cancelled, the pressure reduction on the biological material and the cells can be realized by matching with the resetting part 6, so that the device can easily and dynamically apply pressure on the biological material and the cells, further the proliferation and the differentiation of the cells are influenced, and the cell biomechanical condition is constructed.
In this embodiment, the supporting plate 1 is provided with a plurality of sliding supporting rods 11, and the secondary pressing plate 4 is slidably mounted on the sliding supporting rods 11. The secondary pressure plate 4 is provided with a sliding hole in clearance fit with the sliding support rod 11. The number of the sliding support rods 11 is at least two, and the two sliding support rods 11 are symmetrically arranged relative to the secondary pressing plate 4. The secondary pressing plate 4 can move more stably through the arrangement of the sliding supporting rod 11, and a better loading effect can be achieved.
Specifically, in the present embodiment, the secondary pressing plate 4 is a rectangular plate, and the sliding support rods 11 are disposed at four corners of the secondary pressing plate 4. The sliding support rods 11 are provided with four sliding support rods which are respectively arranged at four corners of the secondary pressing plate 4, and the arrangement of the structure can further ensure the stability of the sliding of the secondary pressing plate 4.
In this embodiment, the reset member 6 is a spring sleeved on the outer side of the sliding support rod 11; two ends of the resetting piece 6 are respectively abutted to the secondary pressure plate 4 and the supporting plate 1. The spring is capable of driving the secondary pressure plate 4 away from the primary pressure plate 3 in a freely extended state.
The dynamic mechanical loading device based on the orifice plate in the embodiment further comprises a bracket 8 fixed on the support plate 1. The bracket 8 includes an upper support plate 81 and a support rod 82 fixedly connecting the upper support plate 81 and the support plate 1. The number of the supporting rods 82 is four, the supporting plate 1 is a rectangular plate, the upper supporting plate 81 is a rectangular plate matched with the supporting plate 1, and the supporting rods 82 are arranged on four corners of the supporting plate 1.
The driving piece passes through driving piece base 51 to be fixed on support 8, and is concrete, and driving piece base 51 is fixed on last backup pad 81, goes up to be provided with on the backup pad 81 with connecting rod 521 clearance fit's support slide opening, connecting rod 521 wears to establish and supports the slide opening.
The link 52 has a T-shaped structure including a link 521 and a slide block 522 perpendicularly connected to the link 521. The driver base 51 is provided with a number of driver slide bars 55 extending in the longitudinal direction. The slide block 522 is slidably mounted on the driver slide 55.
The smooth movement of the connecting element 52 can be ensured by the cooperation between the sliding block 522 and the sliding rod 55 of the driving element, and the data collected by the mechanical sensor 54 can be ensured to be more accurate and reliable.
Specifically, the driving element base 51 is further provided with a first limiting portion 511 and a second limiting portion 512, and the first limiting portion 511 and the second limiting portion 512 are disposed on two sides of the driving element sliding rod 55. The first limiting portion 511 is used to limit the maximum stroke of the connecting rod 521 moving downwards, and the second limiting portion 512 is used to limit the maximum stroke of the connecting rod 521 moving upwards.
In the present embodiment, the driving member base 51 is provided with two grooves extending in the transverse direction, and the two sliding rods 55 are provided, and two ends of the two sliding rods 55 abut against the upper side wall and the lower side wall of the grooves respectively. The first position-limiting portion 511 is a lower sidewall of the groove, and the second position-limiting portion 512 is an upper sidewall of the groove.
The primary pressure plate 3 in this embodiment has a T-shaped structure, and includes a crimping part 31 which is clearance-fitted to the culture chamber 71 and a stopper part 32 which is fixed on the upper side of the crimping part 31, and the diameter of the cross section of the stopper part 32 is larger than that of the cross section of the crimping part 31. The maximum downward pressing stroke of the crimping part 31 can be limited by the arrangement of the limiting part 32, so that the phenomenon that the primary pressing plate 3 excessively presses cell materials in the culture cavity 71 to cause damage is avoided.
In this embodiment the drive member is a linear motor, a pneumatic cylinder or a syringe pump.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (10)
1. A dynamic mechanical loading device based on a pore plate is characterized by comprising a supporting plate, the pore plate arranged on the supporting plate, a plurality of primary pressure plates, a secondary pressure plate arranged above the primary pressure plate, a reset piece arranged between the secondary pressure plate and the supporting plate and a driving unit driving the secondary pressure plate to move towards the direction of the primary pressure plate;
the pore plate is provided with a plurality of accommodating cavities, fixing modules are arranged in the accommodating cavities, culture cavities are arranged on the fixing modules, and the primary pressing plate is matched with the culture cavities;
the driving unit comprises a driving piece, a connecting piece and a driving pressing plate arranged on the connecting piece, the driving pressing plate is arranged on the upper side of the pressing plate, and a mechanical sensor is further arranged between the driving pressing plate and the connecting piece.
2. The orifice plate-based dynamic mechanical loading device of claim 1, wherein a plurality of sliding support rods are disposed on the support plate, and the secondary pressure plate is slidably mounted on the sliding support rods.
3. The orifice plate-based dynamic mechanical loading device of claim 2, wherein the secondary pressure plate is provided with a slide hole in clearance fit with the slide support rod.
4. The orifice plate-based dynamic mechanical loading device of claim 2, wherein the secondary pressure plate is a rectangular plate, and the sliding support rods are disposed at four corners of the secondary pressure plate.
5. The orifice plate-based dynamic mechanical loading device of claim 2, wherein the reset member is a spring sleeved outside the sliding support rod; the two ends of the reset piece are respectively abutted to the secondary pressing plate and the supporting plate.
6. The orifice-plate-based dynamic mechanical loading device of claim 1, further comprising a bracket secured to the support plate;
the driving piece is fixed on the bracket through a driving piece base, and a plurality of driving piece sliding rods extending along the longitudinal direction are arranged on the driving piece base;
the connecting piece has T type structure, including the connecting rod and with the sliding block of connecting rod perpendicular connection, the sliding block is installed on the driving piece slide bar.
7. The orifice plate-based dynamic mechanical loading device of claim 6, wherein the driving member base is further provided with a first limiting portion and a second limiting portion, and the first limiting portion and the second limiting portion are disposed on two sides of the driving member sliding rod.
8. The orifice plate-based dynamic mechanical loading device of claim 6, wherein the bracket comprises an upper support plate and a support rod, the support rod fixedly connecting the upper support plate and the support plate;
the driving piece base is fixed on the upper supporting plate, and a supporting sliding hole in clearance fit with the connecting rod is formed in the upper supporting plate.
9. The orifice plate-based dynamic mechanical loading device of claim 1, wherein the primary pressure plate has a T-shaped structure, and comprises a crimping part which is in clearance fit with the culture cavity and a limiting part which is fixed on the upper side of the crimping part, and the diameter of the cross section of the limiting part is larger than that of the cross section of the crimping part.
10. The orifice plate-based dynamic mechanical loading device of claim 1, wherein the driving member is a linear motor, a pneumatic cylinder, or a syringe pump.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010337928.4A CN111440717A (en) | 2020-04-26 | 2020-04-26 | Dynamic mechanical loading device based on orifice plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010337928.4A CN111440717A (en) | 2020-04-26 | 2020-04-26 | Dynamic mechanical loading device based on orifice plate |
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| Publication Number | Publication Date |
|---|---|
| CN111440717A true CN111440717A (en) | 2020-07-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010337928.4A Pending CN111440717A (en) | 2020-04-26 | 2020-04-26 | Dynamic mechanical loading device based on orifice plate |
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| CN (1) | CN111440717A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112375680A (en) * | 2020-11-17 | 2021-02-19 | 黎汉达 | Biological cell culture device with stress stimulation effect |
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