CN111440701A - Multi-flux cell extraction device - Google Patents
Multi-flux cell extraction device Download PDFInfo
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- CN111440701A CN111440701A CN202010407690.8A CN202010407690A CN111440701A CN 111440701 A CN111440701 A CN 111440701A CN 202010407690 A CN202010407690 A CN 202010407690A CN 111440701 A CN111440701 A CN 111440701A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
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Abstract
The invention relates to a multi-flux cell extraction device, which comprises a base, a first bracket, a second bracket and a third bracket, wherein the first bracket is arranged on the base and is provided with two X-axis axial guide rails and a Y-axis axial guide rail; the second bracket is provided with a sliding groove matched with the Y-axis axial guide rail; the container assembly is arranged on the base and comprises a plurality of sample tube racks; the suction assembly is arranged on the base and is electrically connected with the circuit control assembly; the pipetting assembly is arranged on the second bracket and is connected with the suction assembly through a conduit, and the pipetting assembly is electrically connected with the circuit control assembly; and the spiral cover extracting assembly is arranged on the second support and is electrically connected with the circuit control assembly. The device integrates a plurality of components such as a liquid transfer component, a rotary cover extraction component, a pipette disassembly component and the like, can carry out multi-aspect operation on cell fluid extraction and sample tube management, and really greatly improves the operation efficiency.
Description
Technical Field
The invention relates to a multi-flux cell extraction device, and belongs to the technical field of biomedical equipment.
Background
In the prior art, need monitor through the people in the cell extraction working process, judge the cell layer height that its needs extracted, absorb the transfer through liquid-transfering device, this operation needs the operator to operate in biological safety cabinet, and along with sample quantity increase, the operator needs frequent going on and off the lid, inhales actions such as flowing back, leads to easily drawing cell layer unstability, and frequent operation intensity of labour not only causes fatigue easily but also easily along with the risk that the operator pollutes and bring.
In addition, the existing extraction devices are often operated in a single flux mode, when the extraction devices need to be operated simultaneously in the face of a plurality of samples, the efficiency is low, the structure of the cover screwing mechanism is complex, the occupied space is large, and the cost is high.
Disclosure of Invention
In order to solve the outstanding problems, the invention provides a multi-flux cell extraction device, which is provided with a suction assembly and a liquid transfer assembly matched with the suction assembly, wherein the liquid transfer assembly is provided with a plurality of independently operated liquid transfer pipe clamping parts, so that various cell fluids can be simultaneously and accurately measured, the operation error caused by manually extracting the cell fluids is avoided, and the operation efficiency is improved.
In order to achieve the purpose, the invention adopts the following technical scheme:
a multi-throughput cell extraction device, comprising the following components:
the base plate (1) is provided with a base,
the first support 2 is arranged on the base 1, two X-axis axial guide rails 21 and a Y-axis axial guide rail 22 are arranged on the first support 2, and sliding grooves matched with the X-axis axial guide rails 21 are formed in two ends of the Y-axis axial guide rail 22;
the second bracket 8 is provided with a sliding groove matched with the Y-axis axial guide rail 22;
the circuit control assembly is arranged on the base 1;
the container assembly 6 is arranged on the base 1, and the container assembly 6 comprises a plurality of sample tube racks for accommodating samples;
the suction assembly 3 is arranged on the base 1 and is electrically connected with the circuit control assembly, and the circuit control assembly controls the suction motion of the suction assembly 3;
a pipetting assembly 4 arranged on the second support 8 and connected with the aspirating assembly 3 through a conduit, wherein the pipetting assembly 4 is electrically connected with the circuit control assembly, and the circuit control assembly controls the pipetting assembly 4 to slide along the X-axis axial guide rail 21 and/or the Y-axis axial guide rail 22 for extending the pipetting assembly 4 into the container assembly 6 for aspirating the sample;
and the screw cap extraction assembly 5 is arranged on the second support 8 and is electrically connected with the circuit control assembly, and the circuit control assembly controls the screw cap extraction assembly 5 to screw cap and/or take and place the sample tubes in the container assembly 6.
The multi-flux cell extraction device, preferably, the second support 8 includes a second support upper support plate 81 and a second support base 82, the lower end surface of the second support base 82 is provided with a pipette detaching assembly 9, which includes a fifth motor 91, a sliding plate 92, a base 94 and a fixing plate 93, the fifth motor 91 is fixed on the base 94, the sliding plate 92 is arranged above the base 94, the fixing plate 93 is arranged above the sliding plate 92, and the fixing plate 93 is fixed on the lower end surface of the second support base 82; be provided with gear 941 on the base 94, be provided with rack 923 and disassembly hole 921 on the slide 92, the output of fifth motor 91 is connected gear 941, gear 941 with rack 923 meshes mutually for the drive slide 92 is flexible.
The multi-flux cell extraction device, preferably, move liquid subassembly 4 includes a plurality of first transmission shafts 45, a plurality of first motor 44, a plurality of first slider 41 and with a plurality of the second slider 42 that first slider 41 one-to-one set up, first slider 41 with fixed connection can be dismantled to second slider 42, first slider 41 one-to-one corresponding ring cover is in on first transmission shaft 45, first transmission shaft 45 passes through first belt 46 and connects the output of first motor 44, the lower extreme of second slider 42 is provided with pipette clamping part 43 for the centre gripping pipette.
The multi-throughput cell extraction device preferably further comprises a sample liquid level height detection assembly 10, which is arranged on the first slide block 41 on one side of the pipetting assembly 4, and comprises a first clamping leg 101 and a second clamping leg 102, wherein the first clamping leg 101 and the second clamping leg 102 are parallel to the first transmission shaft 45, a pair of openings are arranged at the lower ends of the first clamping leg 101 and the second clamping leg 102, and a transmitter and a receiver are arranged in the openings.
In the multi-throughput cell extraction device, preferably, the cap-screwing extraction assembly 5 comprises a third bracket 51, and the third bracket 51 comprises a third bracket base 511 and a third bracket upper support plate 512;
the clamping mechanisms are arranged on the third bracket base 511 and comprise a fourth motor 59 and a fourth transmission shaft 591, the output end of the fourth motor 59 is connected with the fourth transmission shaft 591, and a clamping part is arranged on the fourth transmission shaft 591 and used for clamping a sample container;
the cover screwing mechanisms are arranged on the third support base 511 and are in one-to-one correspondence with the clamping mechanisms, each cover screwing mechanism comprises a third motor 53 and a third transmission shaft 531, the output end of the third motor 53 is connected with the third transmission shaft 531, and the third transmission shaft 531 is connected with a gear which is sleeved on the fourth transmission shaft 591 in a ring mode through a second belt 56;
and the lifting mechanism is arranged on the third support upper supporting plate 512 and comprises a second motor 52 and a second transmission shaft 521, the output end of the second motor 52 is connected with the second transmission shaft 521, and the lower end of the second transmission shaft 521 is connected with the third support upper supporting plate 512 and is used for driving the clamping mechanism and the cap screwing mechanism to ascend or descend.
The multi-flux cell extraction device preferably comprises a container bottom plate 62, a container support frame 61, container side plates 64 and a container upper cover plate 63, wherein the container bottom plate 62 is provided with a plurality of fixing columns 621 and a plurality of rows of second tube holes 622, the container upper cover plate 63 is provided with fixing holes 631 and first tube holes 632 which are respectively in one-to-one correspondence with the fixing columns 621 and the second tube holes 622, the container support frame 61 is provided with two container support frame side plates 614, one end of each of the two container support frame side plates 614 is provided with a movable wrench 611 and is also provided with a limiting hole 613 penetrating through the upper end surface and the lower end surface of the container support frame, and a limiting pin is arranged in the limiting hole 613; a plurality of rows of protective sleeves 612 corresponding to the first pipe holes 632 are arranged between the two container support frame side plates 614; the container support frame 61 is sleeved outside the fixing post 621, the container side plate 64 is matched with the container support frame 61, the upper end of the container side plate 64 is covered with the container upper cover plate 63, and the free end of the fixing post 621 is fixed in the fixing hole 631.
The multi-throughput cell extraction device, preferably, the suction assembly 3 includes a fourth bracket 37, a suction tube 34, a sixth transmission shaft 32 and a sixth motor 31, the fourth bracket 37 includes a fourth bracket horizontal support plate 371 and a fourth bracket vertical support plate 372, the sixth motor 31 is fixed on the lower end surface of the fourth bracket horizontal support plate 371, the output end of the sixth motor 31 is connected with the sixth transmission shaft 32, one end of the suction tube 34 is fixed on the fourth bracket vertical support plate 372 through a suction tube fixing column 35, and the other end is connected with the sixth transmission shaft 32 through a screw and a third belt 33.
In the multi-throughput cell extraction apparatus, preferably, the clamping member includes a third slider 54 and three mechanical claws 55, the third slider 54 is in a hollow cylinder shape, and a cavity of the third slider 54 is used for accommodating the fourth transmission shaft 591; the three mechanical claws 55 are uniformly arranged on the outer side wall of the third slide block 54; and/or the presence of a gas in the gas,
the gripper 55 is an ear-shaped structure, and includes a first clamping portion 551 and a second clamping portion 552, the second clamping portion 552 is disposed under the first clamping portion 551, a first clamping surface 555 is disposed on the first clamping portion 551, a second clamping surface 556 is disposed on the second clamping portion 552, and the first clamping surface 555 and the second clamping surface 556 are respectively used for clamping sample container covers with different sizes.
In the multi-throughput cell extraction device, preferably, the first clamping surface 555 and the second clamping surface 556 are respectively provided with a rib 554 for clamping the sample container cover, and the lower end of the second clamping portion 552 is provided with a step 557 protruding toward the axis for clamping the sample container body; and/or the presence of a gas in the gas,
the first clamping portion 551 is provided with an eyelet 553 for fixing the gripper 55 to an outer sidewall of the third slider 54.
In the multi-flux cell extraction device, preferably, the telescopic end of the sliding plate 92 is provided with a groove 922 for receiving leakage of a pipette.
Due to the adoption of the technical scheme, the invention has the following advantages:
1. according to the device, the suction assembly and the liquid transfer assembly matched with the suction assembly are arranged, and the liquid transfer assembly is provided with the plurality of independently operated liquid transfer pipe clamping parts, so that various cell fluids can be simultaneously and accurately measured, the operation error caused by manually extracting the cell fluids is avoided, and the operation efficiency is improved;
2. the cap screwing extraction assembly is provided with the plurality of clamping mechanisms capable of operating independently and the cap screwing mechanisms matched with the clamping mechanisms, so that the cap opening, the cap closing and the extraction of a plurality of sample tubes can be performed simultaneously, and the working efficiency of cap screwing and extraction of the sample tubes is greatly improved;
3. according to the invention, the liquid level height detection assembly of the sample is arranged on the liquid transfer assembly, and the optical fiber, the optical fiber signal transmitter and the optical fiber signal receiver are arranged in the assembly, so that the height of the liquid level of the cells can be monitored in real time, and the cell liquid can be accurately measured;
4. according to the invention, the pipette disassembling component is arranged at the lower end of the pipette component, so that a waste pipette can be quickly disassembled from the device, the pipette does not need to be manually replaced, and the working efficiency is improved;
5. the container support frame is arranged on the sample tube frame, the sample tube protective sleeve, the limiting hole, the limiting pin and the wrench are arranged on the container support frame, the wrench is buckled to control the protective sleeve to clamp or loosen the sample tube, and the problem that the sample tube shakes to cause inaccurate liquid taking amount in the liquid taking process is solved;
6. according to the sample container grabbing device, 3 mechanical claws which are uniformly distributed are arranged on the outer side wall of the third sliding block, a stable triangular structure can be formed when a sample container is grabbed, the three mechanical claws are used for clamping the sample container cover at the same time, the concentricity is high, and the stability of the action of the rotary switch is high; on the other hand, the two clamping parts are arranged on the mechanical claw, and the clamping angles of the clamping surfaces of the two clamping parts are different, so that the sample container covers with different sizes can be clamped; the lower end of the second clamping part is provided with a step, so that the sample container cover can be quickly opened;
7. the motor is driven by the belt pulley, the torque is amplified through the gear ratio of the driving wheel and the driven wheel, a large rotating force can be exerted under the motor with a small specification, and the phenomenon of non-opening rotation cannot be generated in the cap screwing process;
8. the device of the invention integrates a plurality of components such as a liquid transfer component, a rotary cover extraction component, a liquid transfer pipe disassembly component and the like, can carry out operations on cell fluid extraction and sample pipe management in many aspects, really realizes diversification of cell fluid external operation, and greatly improves operation efficiency.
Drawings
FIG. 1 is a schematic view of the overall structure of the apparatus of the present invention;
FIG. 2 is a schematic view of the structure of the pipetting module in the apparatus of the invention;
FIG. 3 is a schematic view of the arrangement of the pipetting module of the apparatus according to the invention in the operating state;
FIG. 4 is a schematic view of a spin-cap extractor assembly of the apparatus of the present invention;
FIG. 5 is a schematic view of a partial structure of a spin-cap extractor assembly of the apparatus of the present invention;
FIG. 6 is a schematic diagram of the structure of a pipette tip remover unit of the apparatus of the present invention;
FIG. 7 is a schematic view of a partial structure of a pipette tip removal assembly of the device of the present invention;
FIG. 8 is a schematic diagram of the configuration of the pumping assembly of the apparatus of the present invention;
FIG. 9 is a schematic diagram of the construction of the container assembly of the apparatus of the present invention;
FIG. 10 is an exploded view of the container assembly of FIG. 9;
FIG. 11 is a schematic diagram of the structure of the sample level detection assembly of the apparatus of the present invention;
FIG. 12 is a schematic view of the gripper of the apparatus of the present invention;
FIG. 13 is a schematic view of another embodiment of the container assembly of the apparatus of the present invention;
FIG. 14 is an exploded view of the container assembly of FIG. 13;
the respective symbols in the figure are as follows:
1-a base; 2-a first support, 21-an X-axis axial guide rail and 22-a Y-axis axial guide rail; 3-suction component, 31-sixth motor, 32-sixth transmission shaft, 33-third belt, 34-suction cylinder, 35-suction cylinder fixing column, 36-catheter accommodating hole, 37-fourth bracket, 371-fourth bracket horizontal supporting plate and 372-fourth bracket vertical supporting plate; 4-pipetting assembly, 41-first slide, 42-second slide, 43-pipette grip, 44-first motor, 45-first transmission shaft, 46-first belt; 5-a rotary cover extracting component, 51-a third bracket, 511-a third bracket base, 512-a supporting plate on the third bracket, 52-a second motor, 521-a second transmission shaft, 53-a third motor, 531-a third transmission shaft, 54-a third slide block, 55-a mechanical claw, 551-a first clamping part, 552-a second clamping part, 553-an eyelet hole, 554-a convex rib, 555-a first clamping surface, 556-a second clamping surface, 557-a step, 56-a second belt, 57-a first horizontal fixing plate, 58-a supporting rod, 59-a fourth motor and 591-a fourth transmission shaft; 6-container assembly, 61-container support frame, 611-wrench, 612-protective sleeve, 613-limiting hole, 614-container support frame side plate, 62-container bottom plate, 621-fixing column, 622-second tube hole, 623-bulge, 63-container upper cover plate, 631-fixing hole, 632-first tube hole and 64-container side plate; 7-a display assembly; 8-a second support, 81-a second support upper support plate, 82-a second support base; 9-pipette removal assembly, 91-fifth motor, 92-slide, 921-removal hole, 922-groove, 923-rack, 93-upper fixed plate, 94-base, 941-gear; 10-a sample liquid level height detection assembly, 101-a first clamping leg, 102-a second clamping leg, 103-a fiber box; 11-cartridge of the cartridge.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention are described clearly and completely below, and it is obvious that the described embodiments are some, not all embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
Example 1
As shown in fig. 1 to 4, the present embodiment relates to a multi-flux cell extraction device, including the following components:
the base plate (1) is provided with a base,
the first support 2 is arranged on the base 1, two X-axis axial guide rails 21 and a Y-axis axial guide rail 22 are arranged on the first support 2, and sliding grooves matched with the X-axis axial guide rails 21 are formed in two ends of the Y-axis axial guide rail 22;
the second bracket 8 is provided with a sliding groove matched with the Y-axis axial guide rail 22;
the circuit control assembly is arranged on the base 1;
the container assembly 6 is arranged on the base 1, and the container assembly 6 comprises a plurality of sample tube racks for accommodating samples;
the suction assembly 3 is arranged on the base 1 and is electrically connected with the circuit control assembly, and the circuit control assembly controls the suction motion of the suction assembly 3;
a pipetting assembly 4 arranged on the second support 8 and connected with the aspirating assembly 3 through a conduit, wherein the pipetting assembly 4 is electrically connected with the circuit control assembly, and the circuit control assembly controls the pipetting assembly 4 to slide along the X-axis axial guide rail 21 and/or the Y-axis axial guide rail 22 for extending the pipetting assembly 4 into the container assembly 6 for aspirating the sample;
and the screw cap extraction assembly 5 is arranged on the second support 8 and is electrically connected with the circuit control assembly, and the circuit control assembly controls the screw cap extraction assembly 5 to screw cap and/or take and place the sample tubes in the container assembly 6.
In this embodiment, it is preferable that a display assembly 7 is further included for displaying the extraction condition of the cellular fluid and controlling the operation of the pipetting assembly 4 and the cap extraction assembly 5.
In this embodiment, preferably, the second support 8 includes a second support upper support plate 81 and a second support base 82, the pipette detaching assembly 9 is disposed on the lower end surface of the second support base 82, and includes a fifth motor 91, a sliding plate 92, a base 94 and a fixing plate 93, the fifth motor 91 is fixed on the base 94, the sliding plate 92 is disposed above the base 94, the fixing plate 93 is disposed above the sliding plate 92, and the fixing plate 93 is fixed on the lower end surface of the second support base 82; be provided with gear 941 on the base 94, be provided with rack 923 and disassembly hole 921 on the slide 92, the output of fifth motor 91 is connected gear 941, gear 941 with rack 923 meshes mutually for the drive slide 92 is flexible.
In this embodiment, preferably, the liquid transfer assembly 4 includes four first transmission shafts 45, four first motors 44, if four first sliders 41 and four second sliders 42 disposed corresponding to the four first sliders 41 one by one, the first sliders 41 and the second sliders 42 are detachably and fixedly connected, the first sliders 41 are sleeved on the first transmission shafts 45 one by one, the first transmission shafts 45 are connected to an output end of the first motors 44 through a first belt 46, and a pipette clamping portion 43 is disposed at a lower end of the second slider 42 for clamping a pipette.
In this embodiment, it is preferable that the sample liquid level detecting assembly 10 is disposed on the first slide 41 on one side of the pipetting assembly 4, and includes a first holding leg 101 and a second holding leg 102, which are parallel to the first transmission shaft 45, and a pair of openings are disposed near the lower ends of the first holding leg 101 and the second holding leg 102, and a transmitter and a receiver are disposed in the openings.
In this embodiment, preferably, the screw cap extraction assembly 5 includes a third bracket 51, and the third bracket 51 includes a third bracket base 511 and a third bracket upper support plate 512;
the four clamping mechanisms are arranged on the third bracket base 511, each clamping mechanism comprises a fourth motor 59 and a fourth transmission shaft 591, the output end of the fourth motor 59 is connected with the fourth transmission shaft 591, and a clamping part is arranged on the fourth transmission shaft 591 and used for clamping a sample container;
the four cover screwing mechanisms are arranged on the third support base 511 and are in one-to-one correspondence with the four clamping mechanisms, each cover screwing mechanism comprises a third motor 53 and a third transmission shaft 531, the output end of the third motor 53 is connected with the third transmission shaft 531, and the third transmission shaft 531 is connected with a gear which is sleeved on the fourth transmission shaft 591 in a ring mode through a second belt 56;
and the lifting mechanism is arranged on the third support upper supporting plate 512 and comprises a second motor 52 and a second transmission shaft 521, the output end of the second motor 52 is connected with the second transmission shaft 521, and the lower end of the second transmission shaft 521 is connected with the third support upper supporting plate 512 and is used for driving the four clamping mechanisms and the four cover screwing mechanisms to ascend or descend.
In this embodiment, preferably, the sample tube rack includes a container bottom plate 62, a container support frame 61, container side plates 64 and a container upper cover plate 63, the container bottom plate 62 is provided with four fixing columns 621 and four rows of second tube holes 622, the container upper cover plate 63 is provided with fixing holes 631 and first tube holes 632 corresponding to the fixing columns 621 and the second tube holes 622 one to one, the container support frame 61 is provided with two container support frame side plates 614, one end of each of the two container support frame side plates 614 is provided with a movable wrench 611 and is further provided with a limit hole 613 penetrating through the upper and lower end faces thereof, and a limit pin is arranged in the limit hole 613; four rows of protective sleeves 612 corresponding to the first pipe holes 632 are arranged between the two container support frame side plates 614; the container support frame 61 is sleeved outside the fixing post 621, the container side plate 64 is matched with the container support frame 61, the upper end of the container side plate 64 is covered with the container upper cover plate 63, and the free end of the fixing post 621 is fixed in the fixing hole 631.
In this embodiment, preferably, the suction assembly 3 includes a fourth bracket 37, a suction cylinder 34, a sixth transmission shaft 32 and a sixth motor 31, the fourth bracket 37 includes a fourth bracket horizontal support plate 371 and a fourth bracket vertical support plate 372, the sixth motor 31 is fixed on the lower end surface of the fourth bracket horizontal support plate 371, the output end of the sixth motor 31 is connected to the sixth transmission shaft 32, one end of the suction cylinder 34 is fixed on the fourth bracket vertical support plate 372 through a suction cylinder fixing column 35, and the other end is connected to the sixth transmission shaft 32 through a screw and a third belt 33.
In this embodiment, preferably, the clamping component includes a third sliding block 54 and three mechanical claws 55, the third sliding block 54 is of a hollow cylinder type, and a cavity of the third sliding block 54 is used for accommodating the fourth transmission shaft 591; the three mechanical claws 55 are uniformly arranged on the outer side wall of the third slide block 54.
In this embodiment, it is preferable that the gripper 55 is an ear-type structure, and includes a first clamping portion 551 and a second clamping portion 552, the second clamping portion 552 is disposed directly under the first clamping portion 551, a first clamping surface 555 is disposed on the first clamping portion 551, a second clamping surface 556 is disposed on the second clamping portion 552, and the first clamping surface 555 and the second clamping surface 556 are respectively configured to clamp sample container covers with different sizes.
In this embodiment, it is preferable that the first clamping surface 555 and the second clamping surface 556 are respectively provided with a rib 554 for clamping the sample container cover, and a lower end of the second clamping portion 552 is provided with a step 557 protruding toward the axial center for clamping the sample container body.
In this embodiment, preferably, the first clamping portion 551 is provided with an eyelet 553 for fixing the gripper 55 to an outer sidewall of the third slider 54.
In this embodiment, the telescopic end of the slide plate 92 is preferably provided with a groove 922 for receiving a leakage of the pipette.
In this embodiment, the sample level detection assembly 10 preferably further comprises a fiber box 103 for accommodating excess optical fibers.
In this embodiment, the apparatus of the present invention preferably further includes 2 gun head cartridges 11, and the number of the gun head cartridges 11 is 2 for accommodating discarded pipette tips and new pipette tips.
In this embodiment, the outer portion of the catheter connecting the aspirating assembly 3 and the pipetting assembly 4 is preferably covered with a hard shell to prevent the problem of inaccurate pipetting caused by shaking of the catheter during pipetting.
In this embodiment, it is preferable that a general motor for driving the X-axis axial guide rail 21 and the Y-axis axial guide rail 22 is further provided.
Example 2
This embodiment provides a method of operating the apparatus of embodiment 1, comprising the steps of:
(1) starting the general motor to enable the second bracket 8 to slide on the X-axis axial guide rail 21 and/or the Y-axis axial guide rail 22 to the position above the sample tube rack, and then starting the second motor 52, wherein the cover screwing mechanism and the clamping mechanism move downwards under the driving of the second motor 52;
(2) after the cap screwing mechanism and the clamping mechanism reach the designated height, the second motor 52 is closed, the fourth motor 59 and the third motor 53 are started, and the mechanical claw 55 clamps the sample container body under the driving of the fourth motor 59 and the third motor 53 and unscrews the sample container cap at the same time;
(3) the first motor 44 and the sixth motor 31 are started to extract the solution in the sample container, and the liquid level height in the sample container is monitored in real time through the sample liquid level height monitoring assembly 10 in the sampling process, so that accurate measurement is realized;
(4) after the sampling operation is finished, the first motor 44 is controlled by the circuit control assembly to drive the pipette disassembling assembly 9 to disassemble the waste pipette, and the waste pipette is put into the gun head box 11 to finish the operation.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (10)
1. A multi-flux cell extraction device, comprising:
a base (1) is arranged on the base,
the first support (2) is arranged on the base (1), two X-axis axial guide rails (21) and a Y-axis axial guide rail (22) are arranged on the first support (2), and sliding grooves matched with the X-axis axial guide rails (21) are formed in two ends of the Y-axis axial guide rail (22);
the second bracket (8) is provided with a sliding groove matched with the Y-axis axial guide rail (22);
the circuit control assembly is arranged on the base (1);
the container assembly (6) is arranged on the base (1), and the container assembly (6) comprises a plurality of sample pipe racks for accommodating samples;
the suction assembly (3) is arranged on the base (1) and is electrically connected with the circuit control assembly, and the circuit control assembly controls the suction motion of the suction assembly (3);
a pipetting assembly (4) arranged on the second bracket (8) and connected with the suction assembly (3) through a conduit, wherein the pipetting assembly (4) is electrically connected with the circuit control assembly, and the circuit control assembly controls the pipetting assembly (4) to slide along the X-axis axial guide rail (21) and/or the Y-axis axial guide rail (22) for extending the pipetting assembly (4) into the container assembly (6) to suck the sample;
and the screw cap extraction component (5) is arranged on the second support (8) and is electrically connected with the circuit control component, and the circuit control component controls the screw cap extraction component (5) to screw cap and/or take and place the sample tubes in the container component (6).
2. The multi-flux cell extraction device according to claim 1, wherein the second support (8) comprises a second support upper support plate (81) and a second support base (82), a pipette detaching assembly (9) is disposed on a lower end surface of the second support base (82), and comprises a fifth motor (91), a sliding plate (92), a base (94) and a fixing plate (93), the fifth motor (91) is fixed on the base (94), the sliding plate (92) is disposed above the base (94), the fixing plate (93) is disposed above the sliding plate (92), and the fixing plate (93) is fixed on a lower end surface of the second support base (82); be provided with gear (941) on base (94), be provided with rack (923) and dismantlement hole (921) on slide (92), the output of fifth motor (91) is connected gear (941), gear (941) with rack (923) meshing mutually is used for the drive slide (92) are flexible.
3. The multi-throughput cell extraction device according to claim 1, wherein the pipetting assembly (4) comprises a plurality of first transmission shafts (45), a plurality of first motors (44), a plurality of first sliders (41), and a plurality of second sliders (42) corresponding to the plurality of first sliders (41) one by one, the first sliders (41) and the second sliders (42) are detachably and fixedly connected, the first sliders (41) are annularly sleeved on the first transmission shafts (45) one by one, the first transmission shafts (45) are connected with the output ends of the first motors (44) through first belts (46), and the lower ends of the second sliders (42) are provided with pipette holders (43) for holding pipettes.
4. The multi-throughput cell extraction device of claim 1, further comprising a sample level height detection assembly (10) disposed on the first slider (41) on one side of the pipetting assembly (4) and comprising a first gripping leg (101) and a second gripping leg (102) parallel to the first transmission shaft (45), wherein a pair of opposing openings are disposed proximate to lower ends of the first gripping leg (101) and the second gripping leg (102), wherein a transmitter and a receiver are disposed within the opposing openings.
5. The multi-throughput cell extraction device of claim 1, wherein the screw-cap extraction assembly (5) comprises a third bracket (51), the third bracket (51) comprising a third bracket base (511) and a third bracket upper support plate (512);
the clamping mechanisms are arranged on the third support base (511) and comprise a fourth motor (59) and a fourth transmission shaft (591), the output end of the fourth motor (59) is connected with the fourth transmission shaft (591), and a clamping part is arranged on the fourth transmission shaft (591) and used for clamping a sample container;
the cover screwing mechanisms are arranged on the third support base (511) and are in one-to-one correspondence with the clamping mechanisms, each cover screwing mechanism comprises a third motor (53) and a third transmission shaft (531), the output end of the third motor (53) is connected with the third transmission shaft (531), and the third transmission shaft (531) is connected with a gear which is sleeved on the fourth transmission shaft (591) in a ring mode through a second belt (56);
elevating system sets up on backup pad (512) on the third support, elevating system includes second motor (52) and second transmission shaft (521), the output of second motor (52) is connected second transmission shaft (521), the lower extreme of second transmission shaft (521) is connected backup pad (512) on the third support for drive a plurality of clamping mechanism and a plurality of rise or descending of spiral cover mechanism.
6. The multi-flux cell extraction device according to claim 1, wherein the sample tube holder comprises a container bottom plate (62), a container support frame (61), container side plates (64) and a container upper cover plate (63), wherein the container bottom plate (62) is provided with a plurality of fixed columns (621) and a plurality of rows of second tube holes (622), the container upper cover plate (63) is provided with a fixed hole (631) and a first tube hole (632) which are respectively in one-to-one correspondence with the fixed columns (621) and the second tube holes (622), the container support frame (61) is provided with two container support frame side plates (614), one end of each of the two container support frame side plates (614) is provided with a movable wrench (611), and the container support frame is further provided with a limit hole (613) penetrating through the upper end surface and the lower end surface of the container support frame, and a limit pin is arranged in; a plurality of rows of protective sleeves (612) corresponding to the first pipe holes (632) are arranged between the two container support frame side plates (614); the container supporting frame (61) is sleeved on the outer side of the fixing column (621), the container side plate (64) is matched with the container supporting frame (61), the upper end of the container side plate (64) is covered with the container upper cover plate (63), and the free end of the fixing column (621) is fixed in the fixing hole (631).
7. The multi-flux cell extraction device according to claim 1, wherein the suction assembly (3) comprises a fourth bracket (37), a suction tube (34), a sixth transmission shaft (32) and a sixth motor (31), the fourth bracket (37) comprises a fourth bracket horizontal support plate (371) and a fourth bracket vertical support plate (372), the sixth motor (31) is fixed on the lower end face of the fourth bracket horizontal support plate (371), the output end of the sixth motor (31) is connected with the sixth transmission shaft (32), one end of the suction tube (34) is fixed on the fourth bracket vertical support plate (372) through a suction tube fixing column (35), and the other end of the suction tube is connected with the sixth transmission shaft (32) through a screw rod and a third belt (33).
8. The multi-throughput cell extraction device of claim 5, wherein the clamping means comprises a third slider (54) and three mechanical jaws (55), the third slider (54) being of the hollow cylinder type, a cavity of the third slider (54) being adapted to receive the fourth drive shaft (591); the three mechanical claws (55) are uniformly arranged on the outer side wall of the third sliding block (54); and/or the presence of a gas in the gas,
the gripper (55) is of an ear-shaped structure and comprises a first clamping portion (551) and a second clamping portion (552), the second clamping portion (552) is arranged under the first clamping portion (551), a first clamping surface (555) is arranged on the first clamping portion (551), a second clamping surface (556) is arranged on the second clamping portion (552), and the first clamping surface (555) and the second clamping surface (556) are respectively used for clamping sample container covers with different sizes.
9. The multi-throughput cell extraction device of claim 8, wherein the first clamping surface (555) and the second clamping surface (556) are respectively provided with a rib (554) for clamping the sample container lid, and the lower end of the second clamping portion (552) is provided with a step (557) protruding toward the axial center for clamping the sample container body; and/or the presence of a gas in the gas,
an ear hole (553) is arranged on the first clamping part (551) and is used for fixing the mechanical claw (55) on the outer side wall of the third slide block (54).
10. The multi-throughput cell extraction device of claim 2, wherein the telescoping end of the slide plate (92) is provided with a groove (922) for receiving a weep from a pipette.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010407690.8A CN111440701A (en) | 2020-05-14 | 2020-05-14 | Multi-flux cell extraction device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010407690.8A CN111440701A (en) | 2020-05-14 | 2020-05-14 | Multi-flux cell extraction device |
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| Publication Number | Publication Date |
|---|---|
| CN111440701A true CN111440701A (en) | 2020-07-24 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010407690.8A Pending CN111440701A (en) | 2020-05-14 | 2020-05-14 | Multi-flux cell extraction device |
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| Country | Link |
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| CN (1) | CN111440701A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113252917A (en) * | 2021-06-01 | 2021-08-13 | 融智生物科技（青岛）有限公司 | Liquid transfer device for test tube |
| CN114395473A (en) * | 2021-11-08 | 2022-04-26 | 温州经川科技有限公司 | Move liquid and divide appearance equipment |
| CN114540157A (en) * | 2022-03-02 | 2022-05-27 | 圣湘生物科技股份有限公司 | Switch lid device and nucleic acid extraction appearance |
| CN114733589A (en) * | 2022-03-03 | 2022-07-12 | 济南市疾病预防控制中心 | Automatic liquid transfer station |
-
2020
- 2020-05-14 CN CN202010407690.8A patent/CN111440701A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113252917A (en) * | 2021-06-01 | 2021-08-13 | 融智生物科技（青岛）有限公司 | Liquid transfer device for test tube |
| CN114395473A (en) * | 2021-11-08 | 2022-04-26 | 温州经川科技有限公司 | Move liquid and divide appearance equipment |
| CN114540157A (en) * | 2022-03-02 | 2022-05-27 | 圣湘生物科技股份有限公司 | Switch lid device and nucleic acid extraction appearance |
| CN114540157B (en) * | 2022-03-02 | 2023-12-05 | 圣湘生物科技股份有限公司 | Cover opening and closing device and nucleic acid extraction instrument |
| CN114733589A (en) * | 2022-03-03 | 2022-07-12 | 济南市疾病预防控制中心 | Automatic liquid transfer station |
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