CN111440217A - Preparation method for producing tea polyphenol and catechin by using waste tea leaves - Google Patents
Preparation method for producing tea polyphenol and catechin by using waste tea leaves Download PDFInfo
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- CN111440217A CN111440217A CN201910042523.5A CN201910042523A CN111440217A CN 111440217 A CN111440217 A CN 111440217A CN 201910042523 A CN201910042523 A CN 201910042523A CN 111440217 A CN111440217 A CN 111440217A
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- C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
- C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
- C07D311/58—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4
- C07D311/60—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2
- C07D311/62—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring other than with oxygen or sulphur atoms in position 2 or 4 with aryl radicals attached in position 2 with oxygen atoms directly attached in position 3, e.g. anthocyanidins
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Abstract
The invention provides a preparation method for producing tea polyphenol and catechin by using waste tea leaves, relates to the field of agriculture, and particularly relates to a preparation method for producing tea polyphenol and catechin by using waste tea leaves. The preparation method of the tea polyphenol comprises the following raw materials in parts by weight: 450-550 parts of tea powder, 2500-3000 parts of distilled water and high-degree grain liquor, 1-2 parts of pectinase, 1-2 parts of cellulase, 1-2 parts of protease and 3-6 parts of edible citric acid; the preparation of the catechin comprises the following raw materials in parts by weight: 45-55 parts of crude tea polyphenol ointment, 400-500 parts of high-purity alcohol, 30-50 parts of phoenix tree sawdust, 0.5-1 part of quicklime and 8-2 parts of edible 9-degree white vinegar. In order to further reduce the cost, waste tea leaves of various large tea buildings and old tea leaves (with higher tea polyphenol content) which are not needed by tea farms are utilized as raw materials in a unique way, so that the problems of raw material waste and environmental pollution are effectively solved, the cost is lower, the recovery rate is higher, and the product is green and environment-friendly.
Description
Technical Field
The present invention relates to the field of agriculture. A method for preparing tea polyphenols and catechin from waste tea is provided.
Background
Tea is a treasure, is a rotten gift of nature and human beings, and has complex chemical components, such as tea polyphenol, purine base, amino acids, protein, pigment, vitamins, mineral elements, tea saponin and the like. The content of tea polyphenol in the tea is generally 15-20%, which is a general term for phenols and derivatives thereof in the tea.
In the early sixties of the twentieth century, japanese scientists found that tea leaf extract contains an antioxidant active ingredient, and first applied it to the field of food antioxidants. Then, scientists in various countries invest in extensive and intensive research, and prove that the polyphenol compound is a polyphenol active compound, namely tea polyphenol, and the catechin content of the polyphenol compound is 45-50%.
With global advocation of the rise and development of green industry and green food, people successively discover that tea polyphenols extracted from natural tea have excellent pharmacological properties and health care effects of antioxidation, radioresistance, anti-mutagenesis, anticancer, anti-aging, antibiosis, sterilization and the like, especially have strong inhibition effect on human cancer cells, so that the tea polyphenols are widely applied and are increasingly attracted by the attention and favor of people.
Actually, the tea polyphenol and catechin content of natural green tea is higher than that of other fermented tea, such as black tea, Pu' er tea and the like.
Catechin, molecular formula: c15h14o6.h2o, molecular weight: 308.28 widely exists in tea, and needle-shaped crystal containing crystal water is obtained from water/acetic acid. But the green extraction process (without adding chemical reagent) of the catechin is very difficult.
Tea polyphenols, especially purified catechins, have the following special effects:
1. has strong effect of eliminating chloasma on skin.
2. Has strong antioxidant and antiaging effects on skin.
3. Has strong radiation resistance to skin.
4. Has strong antibacterial and bactericidal effects on skin.
5. Has strong inhibiting effect on human cancer cells.
6. Has strong inhibitory effect on HIV.
7. Because the tea contains fluorine, the tea can make teeth have stronger resistance to acid erosion and can obviously slow down the periodontitis.
8. In addition, some studies have shown that tea polyphenols have the effects of inhibiting blood pressure (lowering diastolic and systolic pressure) and blood sugar (inhibiting glycolytic enzymes), reducing cholesterol and low-density lipoprotein (L D L) in blood, increasing the amount of high-density lipoprotein (HD L) (used as low-cholesterol egg in Japan), resisting radiation and ultraviolet rays, and resisting cancer (made into health products for preventing cancer in the United states).
In addition, tea polyphenols, especially purified catechin, has effects of resisting aging, resisting inflammation, resisting cardiovascular disease and cerebrovascular disease, preventing and resisting cancer, resisting influenza virus and HIV virus, and preventing diabetes and renal failure. As a health biological product, the health biological product has unique characteristics of no toxicity, low price, oral taking and beauty skin care, and has undergone long-term historical examination in the aspects of pharmacology and pathology.
The antioxidant capacity of catechin is 6-8 times of that of synthetic antioxidants BHT and BHA, 8-12 times of ME and 15-20 times of VC, and the dosage is less: 0.2-0.3% of the composition can act without potential toxic and side effects of the composition. Some cosmetic manufacturers apply the tea polyphenol as an auxiliary material to products, the 3% tea polyphenol additive can improve skin pigmentation, the effective rate on chloasma reaches 80%, and the use performance of cosmetics can be effectively improved. Meanwhile, catechin can also inhibit unsaturated fatty acid in cells from being oxidized by free radicals to generate peroxide and cross-link with membrane protein to form lipofuscin (freckle), delay skin aging, inhibit the activity of allowaminase, whiten skin, inhibit the initiation of ultraviolet rays and other chemical substances, promote hair growth, and prevent and treat skin cancer, skin allergy, dermatitis and other diseases by the activity of epidermal ornithine decarboxylase. The tea multienzyme has great affinity and coagulability to protein keratin in hair and scalp and cells attached to the surface, so that the tea multienzyme has good washing, dandruff removing, hair beautifying and hair care effects.
In advanced western countries such as Europe, America, Japan and the like, a plurality of daily necessities containing catechin are available on the market, which not only enriches the varieties of daily chemical products, but also opens up a new way for the comprehensive utilization of tea.
Disclosure of Invention
The invention aims to provide a preparation method for producing tea polyphenol and catechin by using waste tea aiming at the national conditions of China. Not only can effectively utilize the waste tea leaves of each large tea house and the old tea leaves (with higher tea polyphenol content) unnecessary for tea houses as raw materials, but also can effectively solve the problems of raw material waste and environmental pollution, and has lower cost and higher recovery rate!
In order to achieve the purpose, the technical scheme adopted by the invention is a preparation method for producing tea polyphenol and catechin by using waste tea leaves, wherein the tea polyphenol comprises the following raw materials in parts by weight: 450-550 parts of tea powder, 2500-3000 parts of distilled water and high-degree grain liquor, 1-2 parts of pectinase, 1-2 parts of cellulase, 1-2 parts of protease and 3-6 parts of edible citric acid; the preparation of the catechin comprises the following raw materials in parts by weight: 45-55 parts of crude tea polyphenol ointment, 400-500 parts of high-purity alcohol, 30-50 parts of phoenix tree sawdust, 0.5-1 part of quicklime and 8-2 parts of edible 9-degree white vinegar.
Further, the preparation of the tea polyphenol comprises the following raw materials in parts by weight: 450-550 parts of tea powder, 2500-3000 parts of distilled water and high-degree grain liquor, 1-2 parts of pectinase, 1-2 parts of cellulase, 1-2 parts of protease and 3-6 parts of edible citric acid;
further, the catechin is prepared from the following raw materials in parts by weight: 45-55 parts of crude tea polyphenol ointment, 400-500 parts of high-purity alcohol, 30-50 parts of phoenix tree sawdust, 0.5-1 part of quicklime and 8-2 parts of edible 9-degree white vinegar.
The preparation method for producing the tea polyphenol and the catechin by using the waste tea leaves comprises the following steps:
a. cleaning waste tea leaves recovered from each big tea house and old tea leaves recovered from each big tea house, and then sterilizing by using an ultraviolet sterilizer in the drying process;
b. crushing the sterilized and dried tea leaves into 50-100 meshes of fine powder on a crusher;
c. heating 450-550 parts of tea powder and 2500-3000 parts of distilled water to 40-50 ℃, adding 1-2 parts of pectinase, 1-2 parts of cellulase and 1-2 parts of protease, uniformly stirring, then carrying out heat preservation fermentation for 30 hours, heating to 90-100 ℃ to inactivate enzyme, and then filtering out a filter cake and a first filtrate;
d. adding 2500-3000 parts of grain liquor with the alcohol content of 80% into the filter cake, uniformly stirring, adding 5 parts of edible citric acid, heating to 60-65 ℃, preserving heat for 8-12 hours, and filtering out the filter cake and a second filtrate;
e. continuously adding grain liquor with the same specific height into the filter cake, uniformly stirring, adding 4 parts of citric acid, heating to 60-65 ℃, preserving heat for 6-10 hours, and filtering out the filter cake and a third filtrate; mixing the first filtrate, the second filtrate and the third filtrate, stirring, heating to 60-65 ℃, adding 5 parts of activated carbon for decoloring, and filtering to obtain a light yellow liquid;
f. heating the decolored light yellow liquid to 65-70 ℃, distilling under reduced pressure to recover alcohol, heating to 95-110 ℃, distilling under reduced pressure to recover water, and obtaining 50-55 parts of crude tea polyphenol ointment with the content of 83.8%: wherein, the content of the crude caffeine is 2.33 to 2.4 percent, the content of the fat-soluble pigment is 2.6 to 2.7 percent, the content of the catechin is 49.3 percent, and the balance is water.
g. Adding 45-55 parts of crude tea polyphenol ointment into more than 450-550 parts of reduced pressure distillation recovered alcohol, adding 45-55 parts of phoenix tree sawdust with fineness of about 80 meshes and 1-3 parts of quick lime as an activator, heating to 50-55 ℃, sealing and reacting for 2-3 hours, and filtering out alcohol to obtain first sawdust;
h. adding 25-35 parts of phoenix tree sawdust with the fineness of about 80 meshes and 0.5-1 part of quick lime serving as an excitant into 450-550 parts of the filtered alcohol, heating to 50-55 ℃, sealing and reacting for 1-3 hours, and filtering the alcohol to obtain second sawdust;
i. then heating the filtered alcohol to 65-70 ℃, and carrying out reduced pressure distillation and recovery to obtain high-purity alcohol; finally, adding 45-55 parts of first wood chips, 25-35 parts of second wood chips and 8-12 parts of edible 9-degree white vinegar into 400-500 parts of high-purity alcohol, heating to 45-55 ℃, stirring for 1-2 hours, and filtering the wood chips to obtain a mixed solution containing catechin, an extracting agent and alcohol;
j. distilling the mixed solution at 65-70 deg.C under reduced pressure to recover alcohol to obtain 100-130 parts of theanine ointment; putting 100-130 parts of theanine ointment into an oven, drying at 55-60 ℃ for 2-3 hours to completely evaporate residual alcohol, and obtaining 50 parts of catechin finished products with the purity of 99.9%.
The invention has the following advantages:
1. in the process of extracting tea polyphenol and catechin, toxic and harmful solvents such as acetone, formaldehyde, ethyl acetate, butyl acetate and the like are not added, but edible white spirit is distilled and purified to obtain high-purity alcohol (ethanol) as a green environment-friendly solvent, and the obtained product is safe and nontoxic, can be used as a cosmetic additive at ease and can also be used as an edible health-care product additive;
2. in the process of extracting tea polyphenol, because a plurality of active enzymes and edible citric acid are added, the extraction rate of an active agent of the tea polyphenol is further stimulated, and the yield is greatly improved;
3. in the process of extracting catechin, macroporous resin synthesized by chemical reagents is not used as an adsorbent, but natural phoenix tree sawdust is used as the adsorbent, so that the natural environment-friendly effect of catechin is ensured;
4. not only can completely and effectively treat the desert surface sand inducing sand storm culprit, but also can be used as a valuable resource for replacing building sand;
5. in the process of extracting catechin, industrial acid such as sulfuric acid, hydrochloric acid, glacial acetic acid, p-toluenesulfonic acid and the like is not used as an extraction activator, but edible 9-degree white vinegar is used for replacing the extraction activator, so that the corrosion to equipment is low, and the key point is ecological and environment-friendly.
6. China is a global tea large country, the yield of the tea accounts for 65 percent of the world, in order to further reduce the cost, the invention develops a new way to utilize waste tea leaves of various large tea houses and old tea leaves (the tea polyphenol content is higher) which are not needed by tea farms as raw materials, thereby not only effectively solving the problems of raw material waste and environmental pollution, but also having lower cost and higher recovery rate!
7. The preparation method is simple and reasonable, can ensure the product quality, is easy to operate and has low device investment.
Detailed Description
The present invention is described in further detail below with reference to specific examples.
Example 1: a preparation method for producing tea polyphenol and catechin by using waste tea leaves comprises the following steps of:
the first step is as follows: the preparation process of tea polyphenol comprises the following steps:
a. cleaning waste tea leaves recovered from each big tea house and old tea leaves recovered from each big tea house, and then sterilizing by using an ultraviolet sterilizer in the drying process;
b. crushing the sterilized and dried tea leaves into 50-100 meshes of fine powder on a crusher;
c. heating 500 parts of tea powder and 3000 parts of distilled water to 45-50 ℃, adding 1.5 parts of pectinase, 1.5 parts of cellulase and 1.5 parts of protease, uniformly stirring, fermenting at a constant temperature for 30 hours, heating to 95-100 ℃ to inactivate enzyme, and filtering a filter cake and a first filtrate;
d. adding 3000 parts of high-alcohol-content grain liquor with the alcohol content of 80% into the filter cake, uniformly stirring, adding 5 parts of edible citric acid, heating to 55-60 ℃, preserving heat for 10 hours, and filtering out the filter cake and a second filtrate;
e. continuously adding grain liquor with the same specific height into the filter cake, uniformly stirring, adding 4 parts of citric acid, heating to 60-65 ℃, preserving heat for 8 hours, and filtering out the filter cake and third filtrate; mixing the first filtrate, the second filtrate and the third filtrate, stirring and heating to 65 ℃, adding 5 parts by mass of activated carbon into the filtrate for decolorization, and filtering to obtain light yellow liquid;
f. heating the decolored light yellow liquid to 50-65 ℃, distilling under reduced pressure to recover alcohol, heating to 100 ℃, distilling under reduced pressure to recover water, and obtaining 50 parts of crude tea polyphenol ointment with the content of 83.5 percent: wherein, the content of the crude caffeine is 2.23 to 2.4 percent, the content of the fat-soluble pigment is 2.6 to 2.7 percent, the content of catechin is 49.5 percent, and the balance is water.
The second step is that: the preparation process of catechin is as follows:
g. adding 50 parts of crude tea polyphenol ointment into more than 500 parts of reduced pressure distillation recovered alcohol, adding 50 parts of phoenix tree sawdust with the fineness of about 80 meshes and 2 parts of quicklime as an excitant, heating to 50-55 ℃, sealing and reacting for 2-3 hours, and filtering out alcohol to obtain first sawdust;
h. adding 30 parts of phoenix tree sawdust with fineness of about 80 meshes and 1.5 parts of quicklime serving as an excitant into 500 parts of the filtered alcohol, heating to 50-55 ℃, carrying out sealing reaction for 3 hours, and filtering the alcohol to obtain second sawdust;
i. then heating the filtered alcohol to 65-70 ℃, and carrying out reduced pressure distillation and recovery to obtain high-purity alcohol; finally, adding 45 parts of first wood chips, 35 parts of second wood chips and 10 parts of edible 9-degree white vinegar into 450 parts of high-purity alcohol, heating to 45-55 ℃, stirring for 1 hour, and filtering the wood chips to obtain a mixed solution containing catechin, an extracting agent and alcohol;
j. distilling the mixed solution at 65-70 deg.C under reduced pressure to recover alcohol to obtain 130 parts of theanine ointment; and (3) putting 130 parts of theanine ointment into a drying oven, and drying at 55-60 ℃ for 2-3 hours to completely evaporate residual alcohol to obtain 50 parts of catechin finished products with the purity of 99.9%.
Example 2: a preparation method for producing tea polyphenol and catechin by using waste tea leaves comprises the following steps of:
the first step is as follows: the preparation process of tea polyphenol comprises the following steps:
a. cleaning waste tea leaves recovered from each big tea house and old tea leaves recovered from each big tea house, and then sterilizing by using an ultraviolet sterilizer in the drying process;
b. crushing the sterilized and dried tea leaves into 50-100 meshes of fine powder on a crusher;
c. heating 480 parts of tea powder and 2800 parts of distilled water to 45-50 ℃, adding 1 part of pectinase, 1 part of cellulase and 1 part of protease, uniformly stirring, carrying out heat preservation fermentation for 35 hours, heating to 95-100 ℃ to inactivate enzyme, and filtering out a filter cake and a first filtrate;
d. 2800 parts of high-alcohol grain liquor with the alcohol content of 80% is added into the filter cake, 4 parts of edible citric acid is added after the mixture is uniformly stirred, the temperature is raised to 55-60 ℃, the temperature is kept for 9 hours, and then the filter cake and a second filtrate are filtered;
e. continuously adding grain liquor with the same specific height into the filter cake, uniformly stirring, adding 3 parts of citric acid, heating to 60-65 ℃, preserving heat for 8 hours, and filtering out the filter cake and third filtrate; mixing the first filtrate, the second filtrate and the third filtrate, stirring and heating to 65 ℃, adding activated carbon with the mass of 4 parts of the filtrate for decolorization, and filtering to obtain light yellow liquid;
f. heating the decolored light yellow liquid to 50-65 ℃, distilling under reduced pressure to recover alcohol, heating to 100 ℃, distilling under reduced pressure to recover water, and obtaining 48 parts of crude tea polyphenol ointment with the content of 83.6 percent: wherein, the content of the crude caffeine is 2.35 to 2.4 percent, the content of the fat-soluble pigment is 2.6 to 2.7 percent, the content of the catechin is 48.8 percent, and the balance is water.
The second step is that: the preparation process of catechin is as follows:
g. adding 48 parts of crude tea polyphenol ointment into more than 500 parts of reduced pressure distillation recovered alcohol, adding 40 parts of phoenix tree sawdust with the fineness of about 80 meshes and 1.5 parts of quicklime as an excitant, heating to 50-55 ℃, sealing and reacting for 2.5 hours, and filtering out alcohol to obtain first sawdust;
h. adding 30 parts of phoenix tree sawdust with fineness of about 80 meshes and 1 part of quick lime serving as an excitant into 480 parts of the filtered alcohol, heating to 50-55 ℃, carrying out sealing reaction for 3 hours, and filtering the alcohol to obtain second sawdust;
i. then heating the filtered alcohol to 65-70 ℃, and carrying out reduced pressure distillation and recovery to obtain high-purity alcohol; finally, adding 40 parts of first wood chips, 30 parts of second wood chips and 8 parts of edible 9-degree white vinegar into 430 parts of high-purity alcohol, heating to 45-55 ℃, stirring for 1 hour, and filtering the wood chips to obtain a mixed solution containing catechin, an extracting agent and alcohol;
j. distilling the mixed solution at 65-70 deg.C under reduced pressure to recover alcohol to obtain 120 parts of theanine ointment; and (3) putting 120 parts of theanine ointment into a drying oven, and drying at 55-60 ℃ for 2-3 hours to completely evaporate residual alcohol to obtain 45 parts of catechin finished products with the purity of 99.9%.
Example 3: a preparation method for producing tea polyphenol and catechin by using waste tea leaves comprises the following steps of:
the first step is as follows: the preparation process of tea polyphenol comprises the following steps:
a. cleaning waste tea leaves recovered from each big tea house and old tea leaves recovered from each big tea house, and then sterilizing by using an ultraviolet sterilizer in the drying process;
b. crushing the sterilized and dried tea leaves into 50-100 meshes of fine powder on a crusher;
c. heating 450 parts of tea powder and 2500 parts of distilled water to 45-50 ℃, adding 0.7 part of pectinase, 0.8 part of cellulase and 0.9 part of protease, uniformly stirring, fermenting for 33 hours at a constant temperature, heating to 95-100 ℃ to inactivate enzyme, and filtering out a filter cake and a first filtrate;
d. adding 2500 parts of high-alcohol-content grain liquor with the alcohol content of 80% into the filter cake, uniformly stirring, adding 3.5 parts of edible citric acid, heating to 55-60 ℃, preserving heat for 11 hours, and filtering out the filter cake and a second filtrate;
e. continuously adding grain liquor with the same specific height into the filter cake, uniformly stirring, adding 3 parts of citric acid, heating to 60-65 ℃, preserving heat for 8 hours, and filtering out the filter cake and third filtrate; mixing the first filtrate, the second filtrate and the third filtrate, stirring and heating to 65 ℃, adding activated carbon with the mass of 4 parts of the filtrate for decolorization, and filtering to obtain light yellow liquid;
f. heating the decolored light yellow liquid to 50-65 ℃, distilling under reduced pressure to recover alcohol, heating to 100 ℃, distilling under reduced pressure to recover water, and obtaining 45 parts of crude tea polyphenol ointment with the content of 83.1 percent: wherein, the content of the crude caffeine is 2.21 to 2.3 percent, the content of the fat-soluble pigment is 2.6 to 2.7 percent, the content of the catechin is 48.8 percent, and the balance is water.
The second step is that: the preparation process of catechin is as follows:
g. adding 45 parts of crude tea polyphenol ointment into more than 450 parts of reduced pressure distillation recovered alcohol, adding 45 parts of phoenix tree sawdust with the fineness of about 80 meshes and 1.5 parts of quicklime as an excitant, heating to 50-55 ℃, sealing and reacting for 2-3 hours, and filtering out alcohol to obtain first sawdust;
h. adding 25 parts of phoenix tree sawdust with fineness of about 80 meshes and 1 part of quick lime serving as an activator into 430 parts of the filtered alcohol, heating to 50-55 ℃, carrying out sealing reaction for 3 hours, and filtering the alcohol to obtain second sawdust;
i. then heating the filtered alcohol to 65-70 ℃, and carrying out reduced pressure distillation and recovery to obtain high-purity alcohol; finally, adding 45 parts of first wood chips, 35 parts of second wood chips and 8 parts of edible 9-degree white vinegar into 430 parts of high-purity alcohol, heating to 45-55 ℃, stirring for 1 hour, and filtering the wood chips to obtain a mixed solution containing catechin, an extracting agent and alcohol;
j. distilling the mixed solution at 65-70 deg.C under reduced pressure to recover alcohol to obtain 110 parts of theanine ointment; putting 110 parts of theanine ointment into a drying oven, and drying at 55-60 ℃ for 2-3 hours to completely evaporate residual alcohol to obtain 43 parts of catechin finished products with the purity of 99.9%.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the present invention, and it will be apparent to those skilled in the art that various modifications and variations can be made in the spirit and principle of the present invention and that equivalent modifications and substitutions and the like are included in the scope of the present invention.
Claims (4)
1. A preparation method for producing tea polyphenol and catechin by using waste tea leaves is characterized in that the tea polyphenol is prepared from the following raw materials in parts by weight: 450-550 parts of tea powder, 2500-3000 parts of distilled water and high-degree grain liquor, 1-2 parts of pectinase, 1-2 parts of cellulase, 1-2 parts of protease and 3-6 parts of edible citric acid; the preparation of the catechin comprises the following raw materials in parts by weight: 45-55 parts of crude tea polyphenol ointment, 400-500 parts of high-purity alcohol, 30-50 parts of phoenix tree sawdust, 0.5-1 part of quicklime and 8-12 parts of edible 9-degree white vinegar.
2. The method for preparing tea polyphenol from waste tea leaves as claimed in claim 1, wherein the raw materials comprise, by weight: heating 450-550 parts of tea powder and 2500-3000 parts of distilled water to 40-50 ℃, adding 1-2 parts of pectinase, 1-2 parts of cellulase and 1-2 parts of protease, uniformly stirring, then carrying out heat preservation fermentation for 30 hours, heating to 90-100 ℃ to inactivate enzyme, and then filtering out a filter cake and a first filtrate; adding 2500-3000 parts of high-alcohol-content grain liquor with the alcohol content of 80% into the filter cake, uniformly stirring, adding 5 parts of edible citric acid, heating to 60 ℃, preserving heat for 8-12 hours, and filtering out the filter cake and a second filtrate; continuously adding grain liquor with the same ratio into the filter cake, uniformly stirring, adding 4 parts of citric acid, heating to 60-65 ℃, preserving heat for 6-10 hours, and filtering out the filter cake and a third filtrate; mixing the first filtrate, the second filtrate and the third filtrate, stirring, heating to 60-65 ℃, adding 5 parts of activated carbon for decoloring, and filtering to obtain a light yellow liquid; heating the decolored light yellow liquid to 65-70 ℃, distilling under reduced pressure to recover alcohol, heating to 95-110 ℃, distilling under reduced pressure to recover water, and obtaining 50-55 parts of crude tea polyphenol ointment with the content of 83.5%: wherein, the content of the crude caffeine is 2.33 to 2.4 percent, the content of the fat-soluble pigment is 2.6 to 2.7 percent, the content of the catechin is 49.3 percent, and the balance is water.
3. The method according to claim 1 or 2, wherein the process for preparing catechin comprises: adding 45-55 parts of crude tea polyphenol ointment into more than 450-550 parts of reduced pressure distillation recovered alcohol, adding 45-55 parts of phoenix tree sawdust with fineness of about 80 meshes and 1-3 parts of quick lime as an activator, heating to 50-55 ℃, sealing and reacting for 2-3 hours, and filtering out alcohol to obtain first sawdust; adding 25-35 parts of phoenix tree sawdust with the fineness of about 80 meshes and 0.5-1 part of quick lime serving as an excitant into 450-550 parts of the filtered alcohol, heating to 50-55 ℃, sealing and reacting for-3 hours, and filtering the alcohol to obtain second sawdust; then heating the filtered alcohol to 65-70 ℃, and carrying out reduced pressure distillation and recovery to obtain high-purity alcohol; finally, adding 45-55 parts of first wood chips, 25-35 parts of second wood chips and 8-12 parts of edible 9-degree white vinegar into 400-500 parts of high-purity alcohol, heating to 45-55 ℃, stirring for 1-2 hours, and filtering the wood chips to obtain a mixed solution containing catechin, an extracting agent and alcohol; distilling the mixed solution at 65-70 deg.C under reduced pressure to recover alcohol to obtain 100-130 parts of theanine ointment; putting 100-130 parts of theanine ointment into an oven, drying at 55-60 ℃ for 2-3 hours to completely evaporate residual alcohol, and obtaining 50 parts of catechin finished products with the purity of 99.9%.
4. The method for preparing tea polyphenol and catechin from waste tea leaves as claimed in claim 1 or 2, wherein the pectinase, cellulase, protease, edible citric acid, high grain liquor, quicklime and edible 9 degree white vinegar are all commercially available products; the high-purity alcohol is prepared by self-made distillation and purification to obtain the content of 99 percent, and the phoenix tree sawdust is prepared by self-made crushing, drying and sterilization.
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Application publication date: 20200724 |