CN111436588A - Production process of soy sauce with vinegar flavor - Google Patents

Production process of soy sauce with vinegar flavor Download PDF

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Publication number
CN111436588A
CN111436588A CN202010467397.0A CN202010467397A CN111436588A CN 111436588 A CN111436588 A CN 111436588A CN 202010467397 A CN202010467397 A CN 202010467397A CN 111436588 A CN111436588 A CN 111436588A
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Prior art keywords
soy sauce
fermentation
vinegar
starter propagation
starter
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CN202010467397.0A
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Inventor
豆剑伟
樊君
荣秋亮
伍学明
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Qianhe Condiment And Food Co ltd
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Qianhe Condiment And Food Co ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/50Soya sauce
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12JVINEGAR; PREPARATION OR PURIFICATION THEREOF
    • C12J1/00Vinegar; Preparation or purification thereof
    • C12J1/04Vinegar; Preparation or purification thereof from alcohol
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The invention discloses a production process of soy sauce with vinegar flavor, which comprises the steps of raw material moistening and boiling, starter propagation and fermentation, and is characterized in that mature vinegar grains are added in the starter propagation process and the fermentation process, and the mature vinegar grains are prepared by mixing bran, inoculating acetic acid bacteria and putting brick starter into a pool for fermentation after soybean meal and white granulated sugar are subjected to alcohol fermentation. According to the production process of the soy sauce with the vinegar flavor, mature vinegar mash is used for fermentation during starter propagation, the antibacterial activity of acetic acid is utilized to obviously reduce the number of mixed bacteria of starter propagation of the soy sauce, the quality of the soy sauce is ensured, and the soy sauce is endowed with the vinegar flavor by taking the acetic acid as a main sour substance.

Description

Production process of soy sauce with vinegar flavor
Technical Field
The invention relates to the technical field of food fermentation, in particular to a production process of soy sauce with vinegar flavor.
Background
In general, in the high-salt dilute fermentation of soy sauce, the fermentation conditions are limited, the fermentation is generally a non-closed fermentation environment, so the fermentation conditions are slightly different, the variety and the content of sour substances in the soy sauce are influenced, in addition, the non-sterile environment is adopted during the starter propagation, the mixed bacteria except for aspergillus oryzae are easily polluted, although the mixed bacteria do not mean harmful, the higher the number of the mixed bacteria is, the higher the risk is shown. Therefore, the number of mixed bacteria can be used for measuring the quality of the soy sauce koji, and the currently generally adopted method is to adopt strict sanitation and control measures on the koji making environment, but the investment is high and the effect is limited.
The vinegar is a sour flavoring agent, the taste is sour and mellow, the liquid is fragrant and soft, and the main sour and flavor substances are acetic acid. Vinegar and soy sauce are used as two traditional liquid condiments in China, and are used simultaneously in the preparation of a plurality of dishes, but need to be added respectively.
Disclosure of Invention
In order to solve the problems, the invention provides a production process of soy sauce with vinegar flavor, which is characterized in that mature vinegar mash prepared by vinegar fermentation is added to participate in a koji making process, and the soy sauce mash is mixed to perform a high-salt dilute state fermentation process, so that the mixed bacteria pollution in the koji making process can be greatly reduced, and the soy sauce with vinegar flavor and stable quality is prepared.
The invention is realized by the following technical scheme:
a production process of soy sauce with vinegar flavor comprises the steps of raw material moistening and boiling, starter propagation and fermentation, and is characterized in that mature vinegar grains are added in the starter propagation process and the fermentation process, and are prepared by mixing bran, inoculating acetic acid bacteria and brick starter into a pool for fermentation after soybean meal and white granulated sugar are subjected to alcohol fermentation.
The preparation process of the mature vinegar culture comprises the following steps: 1) crushing the bean pulp into powder, adding water according to the material-water ratio of 1:4-6, adding 0.1% amylase based on the bean pulp, stirring uniformly, boiling for gelatinization and liquefaction for 20-30min, and cooling; 2) adding 20-40% of white granulated sugar and 0.1% of saccharifying enzyme into the gelatinized liquid obtained in the step 1) based on the soybean meal, adjusting the pH to 4.5 +/-0.5, heating to 58-62 ℃, keeping the temperature for 30-40min for saccharification, and cooling; 3) adding 0.1% yeast into the saccharified liquid obtained in the step 2), keeping the temperature at 32 +/-2 ℃, and carrying out alcoholic fermentation for 4-6 days to obtain a wine mash; 4) adding bran into the fermented liquor at a ratio of 4-6:1, adding 2-5% of brick koji and 2-5% of acetic acid bacteria seeds, fermenting for 18-20 days, and turning over every day during the fermentation process.
The raw materials are moistened and cooked as follows: and (3) moistening the bean pulp for 30min according to the material-water ratio of 1:1, wherein the water temperature of the bean pulp for moistening is 50 +/-2 ℃, and the bean pulp after moistening is steamed and boiled for 5-30min under the conditions of 0.1-0.2Mpa, 121-plus-130 ℃ to obtain cooked bean pulp which is cooled.
The starter propagation process comprises the following steps: adding 2-6% of mature vinegar grains into the dry soybean meal and wheat flour according to the proportion of 6-8:4-2, inoculating 0.01% of aspergillus oryzae for starter propagation, wherein the starter propagation is disc starter propagation, and the starter propagation temperature is 28-32 ℃, and the starter propagation time is 40-45 h.
The fermentation process is as follows: mixing 24-28% saline water with soy sauce to obtain starter, fermenting in a fermentation tank for 3-7 days, adding mature vinegar, mixing, and fermenting at 15-35 deg.C for 30min at the initial stage of fermentation for 90-180 days.
The salt water is 1.8-2.2 times of the dry material of the soy sauce finished koji, and the addition amount of the mature vinegar culture is 20-30% of the salt water amount.
Compared with the prior art, the invention has the following advantages and beneficial effects:
1. according to the production process of the soy sauce with the vinegar flavor, mature vinegar mash is used for fermentation during starter propagation, the number of mixed bacteria of starter propagation of the soy sauce can be obviously reduced by utilizing the bacteriostasis of acetic acid, the quality of the soy sauce is ensured, and the prepared soy sauce uses the acetic acid as a main sour substance and is endowed with the soy sauce and vinegar flavor;
2. according to the production process of the soy sauce with the vinegar flavor, mature vinegar grains are used for participating in soy sauce starter making, and high-salt dilute mixed fermentation is carried out, so that the protein in the vinegar grains can be further utilized, amino acid, small molecular peptide and the like are generated, the utilization rate of raw materials is ensured, and the delicate flavor of the soy sauce is improved.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples, and the exemplary embodiments and descriptions thereof are only used for explaining the present invention and are not used as limitations of the present invention.
Example 1
A production process of vinegar-flavored soy sauce comprises the following steps:
(1) fermentation of vinegar:
a, crushing the soybean meal into powder, adding water according to the material-water ratio of 1:6, adding 0.1% amylase according to the soybean meal, uniformly stirring, boiling for 25min, and cooling;
b, adding 40% of white granulated sugar and 0.1% of saccharifying enzyme based on the soybean meal, adjusting the pH to 4.7, heating to 58 ℃, keeping for 30min for saccharification, and cooling;
c, adding 0.1 percent of yeast into the saccharified liquid counted by the bean pulp, preserving heat at 33 ℃, performing alcoholic fermentation, and fermenting for 4 days to obtain wine mash;
d, mixing the wine mash and the bran at a ratio of 4:1, inoculating 2% of brick koji and 2% of acetic acid bacteria seeds, and fermenting in a pool;
and e, turning over the fermented grains every day in the fermentation process of the fermented grains of the vinegar, and fermenting for 20 days.
(2) Preparing soy sauce finished koji:
a, moistening the soybean meal and 50 ℃ hot water for 30min at a ratio of 1: 1;
b, steaming the bean pulp after being moistened with water at the temperature of 121 ℃ under 0.1Mpa for 30min to obtain cooked bean pulp, and cooling;
c, mixing cooked soybean meal with wheat flour, wherein the ratio of the soybean meal (calculated by dry soybean meal) to the wheat flour is 6:4, adding 2% of fermented grains of mature vinegar, inoculating 0.01% of aspergillus oryzae, and carrying out disc starter propagation at the starter propagation temperature of 28 ℃ for 40 hours;
(3) mixing and fermenting: the concentration of saline water is 28 percent, the saline water is 2.2 times of the mass of dry materials of the soy sauce finished koji, the saline water is mixed into koji and then put into a tank for fermentation for 7 days, then mature vinegar culture is added, the addition amount of the mature vinegar culture is 30 percent of the saline water amount, the fermentation temperature is 15 ℃, aeration stirring is carried out for 30min every day in the initial fermentation stage, and the total fermentation time is 180 days;
(4) squeezing: squeezing the fermented soy sauce mash to obtain the flavor soy sauce crude oil.
(5) Sterilization and sedimentation: sterilizing the crude oil at 95 deg.C for 15s, settling, and filtering to obtain soy sauce product with edible vinegar flavor.
Example 2
A production process of vinegar-flavored soy sauce comprises the following steps:
(1) fermentation of vinegar:
a, crushing the soybean meal into powder, adding water according to the material-water ratio of 1:4, adding 0.1% amylase according to the soybean meal, uniformly stirring, boiling for 20min, and cooling;
b, adding 20% of white granulated sugar and 0.1% of saccharifying enzyme based on the soybean meal, adjusting the pH to 5.0, heating to 60 ℃, keeping for 30min, and cooling;
c, adding 0.1 percent of yeast into the saccharified liquid counted by the bean pulp, then carrying out heat preservation at 33 ℃, carrying out alcoholic fermentation for 5 days, and obtaining wine mash;
d, mixing the wine mash and the bran at a ratio of 6:1, inoculating 5% of brick koji and 5% of acetic acid bacteria seeds, and fermenting in a pool;
and e, turning over the fermented grains every day in the fermentation process of the fermented grains of the vinegar, and fermenting for 18 days.
(2) Preparing soy sauce finished koji:
a, moistening the soybean meal and 51 ℃ hot water for 30min at a ratio of 1: 1;
b, steaming and boiling the bean pulp after being moistened with water at the pressure of 0.2Mpa and the temperature of 130 ℃ for 5min to obtain cooked bean pulp, and cooling;
c, mixing cooked soybean meal with wheat flour, wherein the ratio of the soybean meal (calculated by dry soybean meal) to the wheat flour is 7:3, adding 4% of fermented mature vinegar, inoculating 0.01% of aspergillus oryzae, and carrying out disc starter propagation at the starter propagation temperature of 32 ℃ for 42 hours.
(3) Mixing and fermenting: the concentration of saline water is 26%, the saline water is 1.8 times of the mass of dry materials of the soy sauce finished koji, the saline water is mixed into koji and then put into a tank for fermentation for 6 days, then mature vinegar culture is added, the addition amount of the mature vinegar culture is 25% of the saline water amount, the fermentation temperature is 20 ℃, aeration stirring is carried out for 30min every day in the initial fermentation stage, and the total fermentation time is 150 days.
(4) Squeezing: squeezing the fermented soy sauce mash to obtain the flavor soy sauce crude oil.
(5) Sterilization and sedimentation: sterilizing the crude oil at 95 deg.C for 15s, settling, and filtering to obtain soy sauce product with edible vinegar flavor.
Example 3
A production process of vinegar-flavored soy sauce comprises the following steps:
(1) fermentation of vinegar:
a, crushing the soybean meal into powder, adding water according to the material-water ratio of 1:5, adding 0.1% amylase according to the soybean meal, uniformly stirring, boiling for 25min, and cooling;
b, adding 30% of white granulated sugar and 0.1% of saccharifying enzyme based on the soybean meal, adjusting the pH to 4.2, heating to 62 ℃, keeping for 30min, and cooling;
c, adding 0.1 percent of yeast into the saccharified liquid counted by the bean pulp, then carrying out heat preservation at 30 ℃, carrying out alcoholic fermentation for 6 days, and obtaining wine mash;
d, mixing the wine mash and the bran in a ratio of 5:1, inoculating 4% of brick koji and 4% of acetic acid bacteria seeds, and fermenting in a pool;
and e, turning over the fermented grains every day in the fermentation process of the fermented grains of the vinegar, and fermenting for 20 days.
(2) Preparing soy sauce finished koji:
a, moistening the soybean meal and 48 ℃ hot water for 30min at a ratio of 1: 1;
b, steaming and boiling the bean pulp after being moistened with water at the pressure of 0.2Mpa and the temperature of 130 ℃ for 5min to obtain cooked bean pulp, and cooling;
c, mixing cooked soybean meal with wheat flour, wherein the ratio of the soybean meal (calculated by dry soybean meal) to the wheat flour is 8:2, adding 6% of fermented mature vinegar, inoculating 0.01% of aspergillus oryzae, and carrying out disc starter propagation at the starter propagation temperature of 30 ℃ for 45 hours.
(3) Mixing and fermenting: the concentration of the saline water is 24 percent, and the saline water is 2.0 times of the mass of the dry materials of the soy sauce finished koji; mixing saline water into a yeast, putting the yeast into a tank, fermenting for 3 days, and adding mature vinegar culture, wherein the addition amount of the mature vinegar culture is 20% of the saline water amount; the fermentation temperature is 35 deg.C, and the fermentation is performed for 90 days in the initial stage of fermentation by aeration stirring for 30min each day.
(4) Squeezing: squeezing the fermented soy sauce mash to obtain the flavor soy sauce crude oil.
(5) Sterilization and sedimentation: sterilizing the crude oil at 95 deg.C for 15s, settling, and filtering to obtain soy sauce product with edible vinegar flavor.
Comparative example 1:
this comparative example is based on example 3, differing from example 3 in that: the mature vinegar culture is not added during starter propagation.
Comparative example 2:
this comparative example is based on example 3, differing from example 3 in that: and (3) adding mature vinegar grains in the sauce mash fermentation process for mixed fermentation.
Comparing the flavor soy sauce prepared by the processes of examples 1 to 3 with the soy sauce of comparative examples 1 to 2, the parameters of starter-forming bacteria number, organic acid content, amino acid nitrogen content, mouthfeel and the like are shown in table 1:
TABLE 1
Figure BDA0002513122310000041
Figure BDA0002513122310000051
As can be seen from Table 1:
1. the flavored sauces of examples 1-3 had the following characteristics:
① the number of mixed bacteria of the finished koji is one order of magnitude, wherein the higher the addition amount of the mature vinegar culture in the koji making process, the better the bacteriostatic effect.
② the soy sauce with vinegar flavor of examples 1-3 has vinegar flavor but no loss of taste because acetic acid is the main acid substance in soy sauce and accounts for more than 50% of total acid, and the amino acid nitrogen content of soy sauce is different mainly because of the material proportion, and the contents of examples 1-3 are all at a more ideal level, which ensures the delicate flavor of soy sauce.
2. Comparison of the data of example 3 with comparative examples 1 and 2 shows that:
① comparative example 1 when no vinegar residue was added during koji making, the number of mixed bacteria in the soy sauce koji making was increased by one order of magnitude as compared with example 3, which indicates that the number of mixed bacteria became larger.
② comparison example 2 does not use mature vinegar, the lactic acid in the organic acid of the finished soy sauce accounts for 65.48% and is the main acid-forming substance, the mixed fermentation of the mature vinegar in the fermentation of the comparison example 3 uses the acetic acid to account for 53.63% and is the main acid-forming substance, which makes the soy sauce have different taste, the finished soy sauce of the comparison example 3 has a certain acetic acid taste, but the comparison example 2 does not have the same taste, and the comparison example 2 has a small difference of the amino acid nitrogen content compared with the comparison example 3, thus the addition of the mature vinegar in the fermentation process has no influence on the delicate flavor of the soy sauce.
The above-mentioned embodiments are intended to illustrate the objects, technical solutions and advantages of the present invention in further detail, and it should be understood that the above-mentioned embodiments are merely exemplary embodiments of the present invention, and are not intended to limit the scope of the present invention, and any modifications, equivalent substitutions, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.

Claims (6)

1. A production process of soy sauce with vinegar flavor comprises the steps of raw material moistening and boiling, starter propagation and fermentation, and is characterized in that mature vinegar grains are added in the starter propagation process and the fermentation process, and are prepared by mixing bran, inoculating acetic acid bacteria and brick starter into a pool for fermentation after soybean meal and white granulated sugar are subjected to alcohol fermentation.
2. The process for producing a soy sauce with a vinegar flavor according to claim 1, wherein the process for preparing the fermented mature vinegar comprises the following steps: 1) crushing the bean pulp into powder, adding water according to the material-water ratio of 1:4-6, adding 0.1% amylase based on the bean pulp, stirring uniformly, boiling for gelatinization and liquefaction for 20-30min, and cooling; 2) adding 20-40% of white granulated sugar and 0.1% of saccharifying enzyme into the gelatinized liquid obtained in the step 1) based on the soybean meal, adjusting the pH to 4.5 +/-0.5, heating to 58-62 ℃, keeping the temperature for 30-40min for saccharification, and cooling; 3) adding 0.1% yeast into the saccharified liquid obtained in the step 2), keeping the temperature at 32 +/-2 ℃, and carrying out alcoholic fermentation for 4-6 days to obtain a wine mash; 4) adding bran into the fermented liquor at a ratio of 4-6:1, adding 2-5% of brick koji and 2-5% of acetic acid bacteria seeds, fermenting for 18-20 days, and turning over every day during the fermentation process.
3. The process for producing vinegar-flavored soy sauce according to claim 1, wherein the raw materials are boiled in water: and (3) moistening the bean pulp for 30min according to the material-water ratio of 1:1, wherein the water temperature of the bean pulp for moistening is 50 +/-2 ℃, and the bean pulp after moistening is steamed and boiled for 5-30min under the conditions of 0.1-0.2Mpa, 121-plus-130 ℃ to obtain cooked bean pulp which is cooled.
4. The process for producing vinegar-flavored soy sauce according to claim 1, wherein the koji-making process comprises the following steps: adding 2-6% of mature vinegar grains into the dry soybean meal and wheat flour according to the proportion of 6-8:4-2, inoculating 0.01% of aspergillus oryzae for starter propagation, wherein the starter propagation is disc starter propagation, and the starter propagation temperature is 28-32 ℃, and the starter propagation time is 40-45 h.
5. The process for producing vinegar-flavored soy sauce according to claim 1, wherein the fermentation process comprises the following steps: mixing 24-28% saline water with soy sauce to obtain starter, fermenting in a fermentation tank for 3-7 days, adding mature vinegar, mixing, and fermenting at 15-35 deg.C for 30min at the initial stage of fermentation for 90-180 days.
6. The process of claim 5, wherein the amount of saline water is 1.8-2.2 times of the mass of the dry materials of soy sauce koji, and the amount of the fermented mature vinegar added is 20-30% of the amount of saline water.
CN202010467397.0A 2020-05-28 2020-05-28 Production process of soy sauce with vinegar flavor Pending CN111436588A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008061583A (en) * 2006-09-07 2008-03-21 Hatakenaka Shoyu:Kk Fermentative seasoning, method for producing the same, and soy sauce processed product by the method
CN103652810A (en) * 2014-01-02 2014-03-26 资阳临江寺生物科技有限公司 Production process for high-amino acid soy sauce
CN104357306A (en) * 2014-11-28 2015-02-18 山西梁汾醋业有限公司 Method for brewing soybean sauce vinegar
CN106954838A (en) * 2017-02-06 2017-07-18 孙德善 The control method of miscellaneous bacteria during a kind of soy sauce brewing
CN109234136A (en) * 2018-11-20 2019-01-18 覃静欣 A kind of method of light electrolysis speed vinegar brewing

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008061583A (en) * 2006-09-07 2008-03-21 Hatakenaka Shoyu:Kk Fermentative seasoning, method for producing the same, and soy sauce processed product by the method
CN103652810A (en) * 2014-01-02 2014-03-26 资阳临江寺生物科技有限公司 Production process for high-amino acid soy sauce
CN104357306A (en) * 2014-11-28 2015-02-18 山西梁汾醋业有限公司 Method for brewing soybean sauce vinegar
CN106954838A (en) * 2017-02-06 2017-07-18 孙德善 The control method of miscellaneous bacteria during a kind of soy sauce brewing
CN109234136A (en) * 2018-11-20 2019-01-18 覃静欣 A kind of method of light electrolysis speed vinegar brewing

Non-Patent Citations (1)

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Title
王芳: "《调味品制作工艺》", 31 March 2003, 延边人民出版社 *

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