CN111423995A

CN111423995A - Salt-tolerant growth-promoting effect of strain glutamicibacter soli1-3-3 and application thereof

Info

Publication number: CN111423995A
Application number: CN202010242767.0A
Authority: CN
Inventors: 孙燕飞; 王琦琦; 孙杰; 刘思洋; 李杨; 褚贵新
Original assignee: Shihezi University
Current assignee: Shihezi University
Priority date: 2020-03-31
Filing date: 2020-03-31
Publication date: 2020-07-17
Anticipated expiration: 2040-03-31
Also published as: CN111423995B

Abstract

The invention provides a salt-tolerant growth-promoting effect of a strain glutamicibacter soli1-3-3 and application thereof, belonging to the technical field of microorganisms for salt-tolerant promotion of plant growth. The preservation number of the strain glutamicibacter soli1-3-3 is CCTCC M20191071, and the NCBI accession number is as follows: MK 016481. The strain glutamicibacter soli1-3-3 has the capability of fixing nitrogen, dissolving phosphorus and potassium and producing ACC (alpha-amino acid) deaminase; the strain glutamicibacter soli1-3-3 has growth promoting effect on different plants.

Description

Salt-tolerant growth-promoting effect of strain glutamicibacter soli1-3-3 and application thereof

Technical Field

The invention belongs to the technical field of microorganisms for salt tolerance and plant growth promotion, and particularly relates to a salt tolerance and growth promotion effect of a strain glutamicibacter soli1-3-3 and application thereof.

Background

Xinjiang belongs to the Lagerstroemia Asia, has temperate continental climate, and causes Xinjiang soil to show salinization with different degrees due to less rainfall and large evaporation in summer. The salinization of soil seriously restricts the growth of economic crops, is easy to cause diseases and insect pests, reduces the yield and quality of agricultural products, and becomes one of important factors restricting the agricultural development. The existing agricultural improvement method for salinized soil mainly comprises physical (deep ploughing, irrigation and salt elimination, and the like), chemical (soil conditioner), and biological improvement (halophyte, microbial improvement). Physical and chemical improvement methods have more defects, more limiting factors and high physical improvement consumption cost, while chemical improvement is likely to cause secondary pollution to soil if the use of the improving agent is not standard, while biological improvement is an environment-friendly and lasting effective improvement method and can improve the salinized soil through the growth and metabolism of organisms. The halophyte planting can absorb partial salt in the soil and relieve the pressure caused by soil salinization, and the microbial preparation can change the soil granular structure, increase the soil fertility, interact with cash crops, improve the salt tolerance of the crops, promote the plant growth and increase the yield, thereby improving the economic benefit. The search for effective microbial strains which are suitable for the local ecological environment of Xinjiang becomes a more important part in agricultural production.

Disclosure of Invention

In view of the above, the invention aims to provide a salt-tolerant growth-promoting bacterium glutamicibacter soli1-3-3, which has the effects of fixing nitrogen, dissolving phosphorus, dissolving potassium, producing ACC deaminase and the like, and can be used for promoting the growth of various crops.

In order to achieve the above object, the present invention provides the following technical solutions:

the invention provides a salt-tolerant growth-promoting bacterium glutamicibacter soli1-3-3, wherein the preservation number of the strain glutamicibacter soli1-3-3 is CCTCC M20191071.

Preferably, the 16S rDNA gene sequence of the strain glutamicibacter soli1-3-3 is shown in SEQ ID NO. 1.

The invention also provides a nitrogen-fixing, phosphorus-dissolving and potassium-dissolving bacterial agent, and the components of the bacterial agent comprise the strain glutamicibacter soli 1-3-3.

The invention also provides an ACC deaminase-producing microbial inoculum, and the components of the microbial inoculum comprise the strain glutamicibacter soli 1-3-3.

The invention also provides a microbial inoculum for promoting the growth of crops, and the components of the microbial inoculum comprise the strain glutamicibacter soli 1-3-3.

Preferably, the crops include arabidopsis, wheat and cotton.

Preferably, when the crop is arabidopsis, the strain glutamicibacter soli1-3-3 can improve the salt tolerance of arabidopsis and promote the growth of leaves; when the crop is wheat, the strain glutamicibacter soli1-3-3 promotes the growth of wheat roots, increases the fresh weight and promotes the accumulation of proline; when the crops are cotton, the strain glutamicibacter soli1-3-3 increases the fresh weight, dry weight, plant height and fruit branch number of the cotton in the bud period and the boll period, and can improve the yield of the cotton in the boll opening period. When the crop is arabidopsis, 0, 50 and 100mM NaCl stress is applied, and the strain glutamicibacter soli1-3-3 can improve the salt tolerance and promote the growth of leaves; when the crops are wheat, 0, 100 and 2000mM NaCl stress is applied, the growth of roots of the strain glutamicibacter soli is promoted under the condition of 1-3-3, the fresh weight is increased, and the accumulation of proline is promoted; when the crops are cotton, the strain glutamicibacter soli1-3-3 increases the fresh weight, dry weight, plant height and fruit branch number of the crops in bud and flower-bell periods, and can improve the yield of cotton in boll opening period.

Compared with the prior art, the invention has the following beneficial effects: (1) the strain glutamicibacter soli1-3-3 has the capabilities of fixing nitrogen, dissolving phosphorus and potassium, and can promote the absorption of N element by crops and increase the content of available phosphorus and potassium in soil;

(2) the strain glutamicibacter soli1-3-3 has the capability of producing ACC deaminase;

(3) the strain glutamicibacter soli1-3-3 can have remarkable growth promotion effect on arabidopsis, wheat and cotton under the condition of different salt stresses, and is characterized in that when the crop is arabidopsis, 0, 50 and 100mM NaCl stresses are applied, and the strain glutamicibacter soli1-3-3 can improve the salt tolerance and promote the growth of leaves; when the crops are wheat, 0, 100 and 2000mM NaCl stress is applied, the strain glutamicibacter soli1-3-3 can promote the root growth, increase the fresh weight and promote the accumulation of proline; when the crops are cotton, the strain glutamicibacter soli1-3-3 can increase the fresh weight and dry weight of the cotton in bud and boll stages, increase the plant height and fruit branch number and improve the yield in boll opening stage.

Biological preservation information

The strain glutamicibacter soli1-3-3 is preserved in a China center for type culture collection in 12 months and 20 days in 2019, the preservation address is Wuhan university in China, the specific address is eight-way Lodoojia mountain in Wuchang area in Wuhan city, Hubei province, and the preservation number is CCTCC M20191071.

Drawings

FIG. 1 is a graph showing colony morphology of glutamicibacter soli1-3-3 and results of a gram-blue staining;

FIG. 2 is a phylogenetic tree of the glutamicibacter soli 1-3-316SrDNA sequence;

FIG. 3 is a graph showing the effect of glutamicibacter soli1-3-3 on the growth of Arabidopsis;

FIG. 4 is a graph showing the effect on cotton growth after inoculation of glutamicibacter soli1-3-3 on day 65 (boll-bell period), in which A represents the aerial part of cotton treated with the inoculation of glutamicibacter soli1-3-3, and B represents the aerial part of control cotton; a represents the underground part of the cotton after inoculation of glutamicibacter soli1-3-3, and b represents the underground part of the cotton of the control group.

Detailed Description

The strain glutamicibacter soli1-3-3 is preferably obtained by screening from the soil of the rhizosphere of suaeda salsa, and the screening method preferably comprises the following steps: placing 5g soil sample in sterile water, culturing in shaking table at room temperature for 30min, standing for 30s, and subjecting the supernatant to gradientDilute and take out 10^-2、10^-3、10^-4、10^-5The multiplied bacterial solutions of 100 mu L are respectively coated on a beef extract peptone agar culture medium containing 80 g/L NaCl by a dilution coating method, are inversely cultured for 24 hours at the temperature of 28 ℃, single colonies of the salt-tolerant strains obtained by separation and purification are picked and inoculated in the beef extract peptone agar slant culture medium, and are stored for standby at the temperature of 4 ℃.

The salt-tolerant strain obtained by screening by the screening method is preserved by using a slant, and the strain is identified by using morphology and molecular biology. The colony of the strain glutamicibacter soli1-3-3 is shown in figure 1, is round, yellow, thin in texture, regular in edge, smooth in surface and gram-positive bacilli.

The invention preferably uses CTAB method to extract 16S rDNA of the strain glutamicibacter soli1-3-3, uses primer 27F (SEQ ID NO. 2): 5 ' -AGAGAGTTTGATCCTGGCTCAG-3 and 1492R (SEQ ID NO. 3): 5'-GGTTACCTTGTTACGACTT-3' to perform PCR amplification on the strain 16S rDNA, and the reaction system of PCR amplification of the invention preferably comprises DNA template 1 mu L, 2 × Taq PCR Master Mix 10 mu L, 5 mu mol/L primer 27F 1 mu L, 5 mu mol/L1492R 1 mu L, ddH₂O12 mu L, 25 mu L in total, the PCR amplification conditions of the invention are 94 ℃ for 5min, 94 ℃ for 45s, 55 ℃ for 45s, 72 ℃ for 1min, 30 cycles in total, and 72 ℃ for 10 min.

The sequence 16S rDNA obtained by amplification is sent to Shanghai workers for sequencing, and the sequence after sequencing is 1445bp, as shown in SEQ ID NO. 1. The sequence is compared in an EzBioclud database, and a phylogenetic tree of the strain glutamicibacter soli1-3-3 is constructed, and the result is shown in figure 2. The phylogenetic tree is preferably constructed by Mega7 software and a maximum likelihood method, and the result shows that the similarity of the sequence of the strain Glutaminobacter soli1-3-3 and the sequence of the model strain Glutaminobacter soli SYB2(EF660748) is 97.96%, and the phylogenetic tree is identified as Glutaminobacter soli. The invention has uploaded the sequence of the strain into NCBI with the sequence accession numbers: MK 016481.

The strain glutamicibacter soli1-3-3 has the functions of fixing nitrogen, dissolving phosphorus and dissolving potassium, and can be applied to preparation of microbial inoculum with corresponding functions. The preparation method of the microbial inoculum is not particularly limited, and the conventional method in the field can be utilized.

The invention also provides an ACC deaminase-producing microbial inoculum, and the components of the microbial inoculum comprise the strain glutamicibacter soli 1-3-3. The strain glutamicibacter soli1-3-3 has activity of producing ACC deaminase, and can be used for preparing ACC deaminase-producing microbial inoculum. The preparation method of the microbial inoculum is not particularly limited, and the conventional method in the field can be utilized.

Crops of the present invention preferably include Arabidopsis, wheat and cotton. In the invention, when the crop is arabidopsis, the strain glutamicibacter soli1-3-3 can improve the salt tolerance of arabidopsis and promote the growth of leaves; when the crop is wheat, the strain glutamicibacter soli1-3-3 promotes the growth of wheat roots, increases the fresh weight and promotes the accumulation of proline; when the crops are cotton, the strain glutamicibacter soli1-3-3 increases the fresh weight, dry weight, plant height and fruit branch number of the cotton in the bud period and the boll period, and can improve the yield of the cotton in the boll opening period. In the invention, when the crop is Arabidopsis thaliana, preferably 0, 50 and 100mM NaCl simulated stress is applied, and the strain glutamicibacter soli1-3-3 can improve the salt tolerance and promote the growth of leaves; when the crop is wheat, preferably applying 0 and 200mM NaCl simulated stress, the strain glutamicibacter soli1-3-3 can promote the root growth, increase the fresh weight and promote the accumulation of proline; when the crops are cotton, under the natural growth condition, the strain glutamicibacter soli1-3-3 can improve the fresh weight and the dry weight of the cotton in the bud period and the flower-bell period, increase the plant height and the fruit branch number and improve the cotton yield in the boll opening period.

The salt growth-promoting bacterium glutamicibacter soli1-3-3 and the application thereof provided by the present invention will be described in detail with reference to the following examples, but they should not be construed as limiting the scope of the present invention.

Example 1

1. Culture medium

The beef extract peptone medium comprises 10g of peptone, 80g of NaCl, 3g of beef extract and 20g of agar, the pH value is 7.2-7.4, and distilled water is added to the medium until the volume is 1L.

Aberra nitrogen-free medium: mannitol 10g, MgSO₄·7H₂O 0.2g，CaSO₄·2H₂O0.1g，KH₂PO₄0.2g，CaCO₃5g of agar, 20g of agar, pH 7.2-7.4, and adding distilled water to a constant volume of 1L.

Phosphate solubilizing bacteria screening culture medium: glucose 10g, (NH)₄)₂SO₄0.5g，KCl 0.3g，MgSO₄·7H₂O0.3g，Ca₃(PO₄)₂10g，FeSO₄·7H₂O 0.03g，MnSO₄·4H₂0.03g of O, 20g of agar, pH 7.0-7.5, and adding distilled water to a constant volume of 1L.

Potassium-dissolving culture medium: na (Na)₂HPO₄2g，FeCl₃0.005g，CaCO₃0.1g，MgSO₄·7H₂0.5g of O, 1g of potassium feldspar powder, 5g of cane sugar, 18g of agar, pH 7.5 and distilled water to a constant volume of 1L.

Salkowski reaction solution (Fe-HC 1O)₄)：1mL0.5mol/L FeC1₃Solution, 35% HCIO of 50m L₄And (4) solution and mixing.

2. Detection of growth promoting activity of salt-tolerant strain

2.1. detecting the activity of glutamicibacter soli1-3-3 for fixing nitrogen, dissolving phosphorus and dissolving potassium: when the strain grows to the logarithmic phase, inoculating glutamicibacter soli1-3-3 to an arbuscular nitrogen-free culture medium, a phosphate solubilizing bacteria screening culture medium and a potassium solubilizing screening culture medium respectively to observe the growth condition of the strain, wherein the results are shown in table 1:

TABLE 1 Glutamicibacter soli1-3-3 growth promoting Activity assay results

Bacterial strains	Activity of nitrogen fixation	Activity of phosphate solubilizing	Activity of potassium decomposition	IAA producing Activity	Activity of producing ACCD
						1-3-3	+	+	+	-	+

As can be seen from Table 1, the strain Glutaminobacter soli1-3-3 has nitrogen fixation, phosphate solubilization, potassium solubilization, ACC deaminase production activity, and the strain 1-3-3 has no IAA activity.

2.2. Qualitative detection of IAA production activity of strain, detecting IAA production activity of strain by spectrophotometer, inoculating glutamicibacter soli1-3-3 to beef extract peptone culture medium added with 0.1mg/m L L-tryptophan, culturing at 28 deg.C for 24 hr, reacting with Salkowski colorimetric solution 1: 1, dark treating for 30min, and measuring OD₅₃₀. The qualitative detection result of the IAA activity of the strain is shown in the table 1.

2.3. Qualitative detection of strain producing ACC deaminase comprises selecting single colony, performing enrichment culture in beef extract peptone culture medium, culturing at 28 deg.C 180r/min until logarithmic phase of strain growth, centrifuging to collect thallus, treating thallus with 0.1 mol/L Tris-HCl (pH7.6) twice, centrifuging at 10000r/min for 5min, discarding supernatant, suspending thallus in 600 μ l 0.1 mol/L Tris-HCl (pH 8.5)) Adding 30 mul toluene, mixing for 30s, sucking 200 mul into 1.5M L centrifuge tube, adding 200 mul 0.5 mol/L ACC, mixing for 5s, warm bathing at 30 deg.C for 15min, adding 0.56 mol/L HCl, mixing, centrifuging at room temperature 10000r/min for 5min, adding 800 mul 0.56 mol/L HCl into 1M L supernatant, mixing in test tube, adding 300 mul 2, 4-dinitrophenylhydrazine (2% 2, 4-dinitrophenylhydrazine is added with 2 mol/L HCl), mixing, warm bathing at 30 deg.C for 30min, adding 2 OD/L NaOH, measuring₄₅₀The qualitative detection results are shown in table 1.

3. Verification of growth promoting effect of strain

Growth promoting Effect of glutamicibacter soli1-3-3 on Arabidopsis thaliana

The plate test comprises the steps of sucking Arabidopsis seeds with a 200 mu L liquid transfer gun after surface disinfection, planting the Arabidopsis seeds at a position about 2.5cm away from the center, placing 6 seeds on each plate in a 16h dark place, 8h illumination and 18 ℃ illumination incubator for 5 days, selecting strains with growth promoting activity obtained by primary screening when Arabidopsis sprouts grow out of two tender leaves, inoculating the strains on a beef extract peptone culture medium at the center, performing the same operation, inoculating sterile water as a control group CK, setting salinity gradients of 0, 50 and 100mmol for each treatment, and performing 3 parallel treatments, and observing the growth condition of the Arabidopsis after the Arabidopsis grows for 30 days.

The results are shown in FIG. 3: under the stress of 0 and 50mM NaCl, glutamicibacter soli1-3-3 can obviously promote the growth of leaves of arabidopsis thaliana compared with CK, promote the germination of arabidopsis thaliana seeds under 100mM NaCl, and improve the salt tolerance of arabidopsis thaliana, but the arabidopsis thaliana is dead finally due to too high salt concentration.

Growth promoting effects of glutamicibacter soli1-3-3 on wheat

The preparation method comprises inoculating glutamicibacter soli1-3-3 into 100m L beef extract peptone culture medium, placing in shaking table, and culturing at 28 deg.C until the concentration of the broth is 10⁸CFU (Colony Forming Units Colony-Forming Units).

Seed treatment: soaking seeds bought from farmer market in saturated saline solution, removing floating and damaged seeds, and selecting full and complete seeds with similar size; cleaning with sterile water for 3 times, sterilizing with 70% ethanol for 1min, immediately cleaning with sterile water for 5 times, and further cleaning with 0.5% sodium hypochloriteSoaking for 10min, washing with sterile water for 5 times, and dark treating on wet filter paper overnight until the seeds are white. Soaking the exposed seeds in 10⁸The bacterial liquid of the CFU is kept for 4 hours for standby; the strain soaked in sterile water was a control group CK.

And (3) pot culture test, namely sowing the treated white seeds in a flowerpot which is 13cm in diameter and 10cm in height and contains 190g of sterilized vermiculite, wherein the sowing depth is 1cm, 10 seeds are planted in each pot, when seedling emergence is realized, sterile water of 0mM NaCl and 200mM NaCl is poured once every 3d to ensure the soil humidity and the corresponding salt stress concentration, bacterial liquid is poured once every 7d to ensure the stable existence of the flora, 3 treatments are repeated for each treatment, 9 pots are treated for each treatment, the seeds grow for 15d in a room temperature environment, plants are harvested, and the root length, the plant height, the dry weight, the fresh weight, the chlorophyll content and the proline content of the wheat are measured to serve as the growth promoting effect evaluation indexes of the strain glutamicibacter soli 1-3-3.

The results are shown in table 2:

TABLE 2 Effect of glutamicibacter soli1-3-3 on wheat growth under NaCl treatment at different concentrations

Note: CK represents the control group and N represents the percentage increase of the treated group compared with CK.

As can be seen from Table 2, in comparison with the control group, the glutamibacter soli1-3-3 increased 24.3% of the root length of the wheat at 0mM NaCl, 14.6% of the fresh weight, and 22.5% of the proline content, and after treatment with 200mM NaCl, the dry weight of the wheat increased 13.3%, 11.4% of the fresh weight, 2.3% of the plant height, and 2.9% of the proline content in the strain 1-3-3 were increased as compared with the CK wheat in the control group.

Growth promoting Effect of glutamicibacter soli1-3-3 on Cotton

The strain seed solution is prepared by culturing glutamicibacter soli1-3-3 in 500m L beef extract peptone culture medium at 28 deg.C until logarithmic phase, and activatingThe number of bacteria is about 10⁹CFU, need to use sterile water to dilute ten times.

Selecting a cotton test field of Kaihe university, selecting a film six-row mode, dividing a 3m area with 1.5m in a film, selecting cotton which grows for 30d to a seedling stage and has the same growth vigor, wherein the cotton in the area with 2m area with 1.5m at the front section is irrigated with bacteria liquid, the cotton in the area with 2m area with 1.5m at the rear section is irrigated with sterile water, each cotton plant is 200m L, the area with 1m area with 1.5m at the middle is not treated, a treatment group and a control group CK are isolated, the bacteria liquid is irrigated once every 15 days and is irrigated for three times continuously, so that the strain is ensured to be stably existed at the rhizosphere of the cotton, 25d (bud stage) from the first irrigated bacteria liquid, 43d (flower boll stage), 65d (boll stage), the height, fresh weight, dry weight, leaf surface area and fruit branch number of the cotton plant of the treatment group and the control group are irrigated once, the growth difference of the cotton plant is measured, the cotton plant weight is randomly collected after 112 days (boll stage), the cotton plant weight is measured, the cotton weight of the cotton is measured, the weight of the cotton, the cotton boll weight of the cotton is measured, and the weight:

TABLE 3 Effect of the strains on Cotton growth at different growth times

TABLE 4 Effect of glutamicibacter soli1-3-3 on Cotton yield

25 days after the cotton spacing is irrigated with bacterial liquid for the first time (bud period), the plant height of the glutamicibacter soli1-3-3 treated cotton is increased by 8.38 percent compared with the control group, the leaf area is increased by 7.70 percent, the fresh weight is increased by 7.70 percent, the single-boll weight is increased by 35.23 percent, and the fruit branch number is increased by 22.17 percent compared with the control group; at 43 days (boll stage) after the first time of pouring the bacterial liquid, the plant height of the cotton is increased by 19.28 percent, the leaf area is increased by 20.22 percent, the fresh weight is increased by 55.46 percent, the dry weight is increased by 47.09 percent, the single boll weight is increased by 26.07 percent, and the number of fruit branches is increased by 24.01 percent. After 65 days from the first irrigating bacterial liquid (in the boll period), the height of the cotton plant is increased by 24.25%, the fresh weight is increased by 40.10%, and the number of fruit branches is increased by 22.79% compared with the control. After the bacterial liquid is poured for 112 days (boll opening period), the influence of the strains 1-3-3 on the cotton yield is measured, and the table 4 shows that the cotton seed cotton weight poured by the strains 1-3-3 is increased by 5.7 percent, the bark surface is increased by 4.1 percent, the cotton seed weight is increased by 10 percent and the single boll weight is increased by 25.4 percent compared with the control.

The invention provides a strain of glutamicibacter soli1-3-3 and application thereof, wherein the strain of glutamicibacter soli1-3-3 has the capabilities of fixing nitrogen, dissolving phosphorus and potassium and producing ACC (ACC deaminase); the glutamicibacter soli1-3-3 has growth promotion effects on different plants, can promote growth of arabidopsis leaves under stress of 0 and 50mM NaCl, improve salt tolerance of arabidopsis under stress of 100mM NaCl, and promote germination of arabidopsis; 0 and 200mM NaCl promote the growth of wheat roots, increase the fresh weight and promote the accumulation of proline; the cell experiments verify that under the natural environment condition, the glutamicibacter soli1-3-3 can improve the fresh weight and the dry weight of cotton in the seedling stage and the bud stage, increase the plant height and the fruit branch number and improve the yield in the boll opening stage.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Sequence listing

<110> river university

Salt-resistant growth-promoting effect of <120> strain glutamicibacter soli1-3-3 and application thereof

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gagcgaacag gattagatac cctggtagtc catgccgtaa acgttgggca ctaggtgtgg 780

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Claims

1. The salt-tolerant growth-promoting bacterium glutamicibacter soli1-3-3 is characterized in that the preservation number of the strain glutamicibacter soli1-3-3 is CCTCC M20191071.

2. The strain Glutaminobacter soli1-3-3 according to claim 1, wherein the 16S rDNA gene sequence of Glutaminobacter soli1-3-3 is shown in SEQ ID No. 1.

3. A nitrogen-fixing, phosphorus-dissolving and potassium-dissolving bacterial agent, which is characterized in that the components of the bacterial agent comprise the strain glutamicibacteroli 1-3-3 of claim 1 or 2.

4. An ACC-producing deaminase bacterial agent comprising the strain Glutaminobactersoli 1-3-3 according to claim 1 or 2 as a component.

5. A microbial inoculum for promoting the growth of crops, which comprises the strain glutamicibacteroli 1-3-3 of claim 1 or 2.

6. The plant growth promoting inoculant according to claim 5, wherein the crops comprise Arabidopsis thaliana, wheat and cotton.

7. The plant growth promoting microbial inoculum according to claim 6, wherein when the crop is arabidopsis thaliana, the strain glutamicibacteroli 1-3-3 can improve the salt tolerance of arabidopsis thaliana and promote the growth of leaves; when the crop is wheat, the strain glutamicibacterosoli 1-3-3 promotes the growth of wheat roots, increases fresh weight and promotes the accumulation of proline; when the crops are cotton, the strain glutamicibacteroli 1-3-3 increases the fresh weight, dry weight, plant height and fruit branch number of the cotton in the bud period and the boll period, and can improve the yield of the cotton in the boll opening period.