CN111408429B - Pipette support for experiment and experiment method - Google Patents
Pipette support for experiment and experiment method Download PDFInfo
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- CN111408429B CN111408429B CN202010265800.1A CN202010265800A CN111408429B CN 111408429 B CN111408429 B CN 111408429B CN 202010265800 A CN202010265800 A CN 202010265800A CN 111408429 B CN111408429 B CN 111408429B
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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Abstract
The invention discloses a pipette support for experiments and an experiment method, which belong to the field of experimental tools, and comprise a support seat, wherein a plurality of insertion holes for inserting pipettes are arranged on the support seat, the axes of the insertion holes are mutually intersected below the support seat, the upper side surface of the support seat is a cambered surface with gradually reduced height from the middle to two sides, when the pipette of the scheme is used for carrying out experiments on mixed liquid, only a mixing container is placed below the support seat, and liquid dripping is carried out on each pipette on the support seat in sequence, so that liquid with different components can be dripped into the same mixing container, the pipette does not need to be repeatedly taken down from the pipette support, the operation is convenient, and the problems that the operation is complex and the operation errors are easily generated in the experiments on the mixed liquid in the prior art are solved.
Description
Technical Field
The invention relates to an experimental tool, in particular to a pipette support for experiments and an experimental method.
Background
The pipette is a very common experimental tool in the fields of medicine, chemistry and the like, is mainly used for mixing various liquids, and can accurately control the amount of each component in the liquid mixing process through the pipette, so that the content of each component in the mixed liquid is ensured.
In order to ensure that the amount of liquid dripped by the pipette can be accurately controlled when the pipette is used, the pipette needs to be placed on a pipette support for standby, and the pipette is prevented from being damaged by overturning.
In the case of performing an experiment of mixing liquids, for example, an experiment in which preferable values of contents of respective components of a mixed liquid are to be investigated, it is necessary to add a plurality of liquids in different contents in different mixing containers, respectively, and to obtain the preferable contents by comparing the results. In the process of experiment, because every kind of liquid all need be used repeatedly, need repeatedly take off the pipette that is equipped with corresponding liquid from the pipette support, the operation is very inconvenient to in the in-process of taking out the pipette repeatedly, forget easily whether certain liquid has added the mixing vessel, lead to the experiment result inaccurate.
In general, in an experiment for exploring the preferred content of each component of the mixed liquid, since different contents of each component need to be verified respectively to select the preferred content, when the number of components in the mixed liquid is large and the selection range of the content of each component is large, the preferred content of each component can be selected only by performing experiments for a large number of times.
For example, the pipette holder disclosed in the chinese patent document with the publication number CN203342829U, which includes a base, a base pillar, a first positioning disc, a second positioning disc, a first connecting part, etc., has disadvantages in that when the pipette holder is used to perform an experiment for exploring the preferred content of each component of a mixed liquid, the pipette needs to be repeatedly removed from the pipette holder, which causes inconvenience in operation, and in the process of repeatedly removing the pipette, it is easy to forget whether a certain liquid has been added to the mixing container, which causes inaccurate experiment results.
For example, in the Chinese patent literature, "oral suspension of lyophilized preparation of liposome-encapsulated insulin" and its preparation process are disclosed, which is published under the publication No. CN102144968B, and contains the following specific components in millimole (m mol) per 1L oral aqueous suspension: 5-50 parts of lecithin; 5-50 parts of cholesterol; 5-50 parts of polyethylene glycol fatty acid ester; 0.1-0.5 of vitamin E; 1-10 parts of insulin; 1-20 parts of sodium chloride; 1-80 parts of disodium hydrogen phosphate and sodium dihydrogen phosphate buffer solution; if the preferred content of each component of the oral suspension needs to be determined, due to the fact that the number of the components is large, a large number of control groups need to be established, the preferred content of each component can be obtained only by comparing the results of the determination of the insulin encapsulation rate of each control group, and therefore the experimental steps are multiple and the time is long.
Disclosure of Invention
The invention provides a pipette bracket for an experiment and an experiment method, aiming at overcoming the problems that in the prior art, in an experiment for researching the optimal values of the contents of all components of a mixed liquid, a large number of control groups need to be set, and a pipette needs to be continuously taken down from the pipette bracket in the liquid dropping process, so that the operation is very complicated and complicated, and the spent experiment time is very long.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention discloses a pipette support for experiments, which comprises a support seat, wherein a plurality of insertion holes for inserting pipettes are formed in the support seat, the axes of the insertion holes are intersected below the support seat, and the upper side surface of the support seat is an arc surface with the height gradually reduced from the middle to two sides.
The common pipettes comprise a slender liquid dropping part and a spherical liquid storage part, and if a plurality of pipettes are vertically placed on the pipette support, the liquid dropping parts of the pipettes are far away from each other due to the large horizontal space occupied by the liquid storage part, so that the liquid cannot be dropped into the same mixing container; in the scheme, after the pipettes are inserted into the insertion holes, the axes of the liquid dropping parts of the pipettes are basically overlapped with the axes of the insertion holes, so that the liquid dropping parts of the pipettes on the supporting seat can be converged below the supporting seat, and the liquid in different pipettes can be dropped into the same mixing container at a short distance; when the pipette in the scheme is used for an experiment for exploring the preferable content of each component of the mixed liquid, the liquid serving as different components can be dripped into the same mixed container by only placing the mixed container below the supporting seat and sequentially controlling each pipette on the supporting seat to perform liquid dripping, the pipette does not need to be repeatedly taken down from the pipette bracket, and the operation is convenient; in addition, the pipette is not required to be taken or placed, and only liquid dripping is required to be carried out in sequence, so that the problem that whether liquid is dripped or not is forgotten is avoided, misoperation is reduced, and the experimental result is more stable; the upper side surface of a common pipette support is a plane, and when the directions of the insertion holes corresponding to different pipettes are different, the inclination angles of the pipettes are different, so that the heights of the bottoms of the pipettes are different; if the selected mixing container is higher, the pipette with the bottom at the lower position is easy to touch the liquid level of the mixed liquid, so that the pipette is polluted, and the dropping liquid amount is inaccurate; if the selected mixing container is low in height, the liquid drops may not accurately drop into the mixing container in the process of dropping liquid by the pipette with the bottom at the high position, so that the experimental result is inaccurate. In the scheme, the upper side face of the supporting seat is made into the cambered surface which is gradually reduced from the middle to the two sides, so that the bottoms of different liquid dropping pipes are approximately at the same height, and the height of the mixing container is conveniently selected.
Preferably, the two sides of the supporting seat are provided with supporting rods, the supporting rods are transversely erected on a bracket, a plurality of bayonets are arranged at intervals in the longitudinal direction of the bracket, and the supporting rods are matched with the bayonets; through inciting somebody to action in the bayonet socket of different positions is put into to the bracing piece, can be on vertical removal supporting seat to carry out the dropping liquid in proper order to the mixing container who places the difference, can be with the vertical a plurality of mixing container who arranges when the dropping liquid record in proper order as No. 1 container, No. 2 container and so on, need not to get the mixing container of difference from the supporting seat below repeatedly and put, thereby avoided leading to mixed liquid to spill when removing mixing container, also avoided mixing each component proportion that mixing container corresponds.
Preferably, the cross section of the support rod and the cross section of the bayonet are square; the square cross section of the supporting rod and the cross section of the bayonet can prevent the supporting rod from rotating in the bayonet, so that the problem that the supporting seat is unstable in supporting is caused.
Preferably, an arc-shaped sinking platform matched with the liquid storage part of the pipette is arranged on the upper side of the insertion hole; the arc sinks the platform and can make the stock solution portion sink in the supporting seat partly when being located on the supporting seat, has increased the arc sink the platform with the area of contact of supporting seat has avoided the supporting seat to remove the time-shift liquid pipe and has dropped from the supporting seat.
Preferably, the support comprises a base, a vertical pillar and a longitudinal support, the bayonet is positioned on the longitudinal support, and a container groove for placing a container for mixing liquid is formed in the base; the possibility of the mixing container turning over due to limb collision can be reduced by preventing the mixing container from being in the container groove, and the base can prevent liquid from dropping on a table top to cause pollution.
Preferably, the base is provided with a supporting platform for placing experimental tools; the saddle can be used to place experimental apparatus such as stirring rod to it is more convenient to get experimental apparatus when making the experiment.
The invention discloses an experimental method, wherein a pipette bracket used for the experiment is the pipette bracket for the experiment, and the method comprises the following steps:
setting a plurality of control groups, selecting different preselected contents for each component of the mixed liquid, wherein the preselected contents of the same liquid in different control groups have different values, respectively marking the liquid of each component of the mixed liquid as liquid 1 and liquid 2 … …, and the like, marking the control groups containing the liquids with different contents as group A and group B … …, and the like, marking the content of the liquid 1 in the group A as a1, the content of the liquid 2 in the group A as a2, the content of the liquid 1 in the group B as B1, and the content of the liquid 2 in the group B as B2 … …, and the like;
respectively sucking the liquid serving as each component of the mixed liquid into a pipette, and respectively inserting the pipette filled with different liquids into the insertion holes of the supporting seat;
placing empty mixing containers with the same quantity as the reference group into the container groove, and dripping the liquid of each component into the first mixing container according to the content of the liquid of each component in the group A;
fourthly, the support seat is lifted and placed into the next bayonet, so that the lower end of each pipette is aligned to the second mixing container, and the liquid of each component is dripped into the second mixing container according to the content of the liquid of each component in the group B; repeating the above process until all the control groups have been added;
adding a solvent into each mixing container, stirring and mixing to ensure that the total amount of liquid in each mixing container is the same after mixing;
sixthly, carrying out target detection on the mixed liquid groups, and selecting the content of each component in the mixed liquid with the best detection result as an optimal content approximate value.
When a commonly-used variable control method is used for carrying out an experiment of the preferred content of each component of the mixed liquid, the content of other components needs to be fixed, the content of one component is changed, and the experiment results are compared to determine the preferred value of the content of the component, when more components exist and the selection range of the content of the components is larger, the number of required control groups is very large, so that the time spent in the experiment is very long; however, for the actual efficacy of the mixed liquid, the influence of the content of each component is different, the influence of the individual components on the actual efficacy of the mixed liquid is large, and the influence of part of the components on the actual efficacy of the mixed liquid is relatively small; when a controlled variable method is used for an experiment, the actual significance of content optimization of the components with small influence on the actual efficacy of the mixed liquid is small, but the number of the control groups cannot be reduced because the components with small influence on the actual efficacy of the mixed liquid cannot be judged before the experiment. In the scheme, the content of any one component in each group is different, and after mixing, the detection result of the group with the preferred value of the component with larger influence on the actual efficacy of the mixed liquid is better than that of the group with the preferred value of the component with smaller influence on the actual efficacy of the mixed liquid, so that the preferred value of the component with larger influence on the actual efficacy of the mixed liquid can be quickly reserved, and the preferred content of each component of the mixed liquid can be quickly selected; for example, when the composition of the suspension in the patent document "oral suspension of liposome-encapsulated insulin lyophilized preparation and process for preparing the same" is optimized, if the experiment is performed by using a method of controlling variables and selecting five contents in the range of the contents of each component, the number of control groups to be performed is 35 groups due to seven components; when the method of the scheme is used for optimization, if five contents are selected in the content range of each component for experiment, only 5 groups are needed; if 10 contents are selected within the range of each component content for the experiment, only 10 groups need to be set, and the component having a large influence on the actual efficacy of the mixed liquid can be selected to a more accurate preferred value.
Preferably, after the step (c) is finished, the method further comprises the following steps:
seventhly, selecting the mixed liquid with the best detection result as an X group, wherein the content of each component liquid is X1, the content of each component liquid is X2 … …, and the like, selecting the mixed liquid with the second best detection result as a Y group, the content of each component liquid is Y1, and the content of each component liquid is Y2 … …, and the like;
taking the content Y1 of the liquid 1 in the group Y as the content of the liquid 1, taking the content of other liquids except the liquid 1 in the group X as the content of other liquids, and recording the content as the group X1; recording the content Y2 of the liquid 2 in the group Y as the content of the liquid 2 and the content of the liquid other than the liquid 2 in the group X as the content of the other liquid as the group X2; … … and so on to form a new control group;
ninthly, placing empty mixing containers with the same number as the new control group into the container tank, and dripping the liquid of each component into the first mixing container according to the content of the liquid of each component in the X1 group;
r c then lifting the support base into the next bayonet, thereby aligning the lower end of each pipette with the second mixing vessel, dropping the liquid of each component into the second mixing vessel at the content of the liquid of each component in group X2; repeating the above process until all new control groups have been added;
⑪ adding solvent into each mixing container, stirring and mixing to ensure that the total amount of liquid in each mixing container is the same as the total amount of liquid in each mixing container in the fifth step;
⑫ performing target detection on each mixed liquid, selecting new control group with detection result better than that of group X, using the component content of group Y as the component content of the preferred group, using the other component content of the preferred group as the component content of the corresponding component in group X, and using the component content of each component of the preferred group as the corrected value of the preferred content.
When a plurality of components which have great influence on the actual efficacy exist in the mixed liquid, the preferred values of some components which have great influence on the actual efficacy in the preferred content in the step (c) are removed, so that the space for improving the obtained preferred content is still large; and two groups of optimal values with the best detection result are reserved by the method, the component content in the group Y superior to the group X is combined with the original component content in the group X, so that a new optimal content correction value is obtained, and the actual efficacy of the mixed liquid corresponding to the optimal content correction value is further improved on the basis of the actual efficacy of the mixed liquid corresponding to the optimal content obtained in the step (sixty).
Therefore, the invention has the following beneficial effects: (1) when the liquid mixing experiment is carried out, the pipette does not need to be repeatedly taken down from the pipette bracket, so that the operation is greatly simplified, and misoperation is not easy to generate; (2) the mixing container does not need to be repeatedly taken and placed, so that the influence on the experimental accuracy caused by the liquid spilled from the mixing container is avoided; (3) when the experiment of the preferable content of each component of the mixed liquid is carried out, the number of experimental groups can be greatly reduced, thereby reducing the time spent in the experiment.
Drawings
FIG. 1 is a schematic diagram of an embodiment of the present invention.
Fig. 2 is a cross-sectional view of the present invention.
In the figure: 1. the device comprises a support 11, a base 12, a longitudinal support 13, a vertical support 14, a bayonet 15, a container groove 16, a saddle 2, a support seat 21, a support rod 22, a jack 23, an arc-shaped sinking platform 3, a pipette 31, a liquid dropping part 32, a liquid storage part 4 and a mixing container.
Detailed Description
The invention is further described with reference to the following detailed description and accompanying drawings.
In the embodiment shown in fig. 1-2, an experimental pipette holder comprises a holder 1 and a support base 2, said holder comprising a base 11; the direction from the left side to the right side is taken as the transverse direction, the direction from the front side to the rear side is taken as the longitudinal direction, and the direction from the upper side to the lower side is taken as the vertical direction; the left side and the right side of the bracket are respectively provided with a longitudinal support 12, the front side and the rear side of each longitudinal support are respectively connected with a vertical support 13, the longitudinal supports are connected with the base through the vertical supports, and the longitudinal supports are provided with bayonets 14 which are longitudinally arranged at intervals and have square sections; the left side and the right side of the supporting seat are respectively provided with a supporting rod 21 with a square cross section and matched with the bayonet, and the supporting seat is transversely placed on the bracket through the supporting rods; the upper side surface of the supporting seat is an arc surface with gradually reduced height from the middle to two sides, the curvature center of the arc surface is superposed with the intersection points of the axes of the plurality of insertion holes, the supporting seat is provided with 5 insertion holes 22 which are transversely arranged at intervals and used for inserting a pipette, wherein the position of the insertion hole positioned at the middle of the 5 insertion holes is positioned at the highest position of the arc surface, the aperture of the insertion hole is matched with the outer diameter of a liquid dropping part 31 of the pipette 3, the upper side of the insertion hole is provided with an arc-shaped sinking platform 23 matched with a liquid storage part 32 of the pipette, and the axes of the plurality of insertion holes are mutually intersected below the supporting seat; the base is provided with a longitudinal container groove 15 for placing the mixing container 4, the base is provided with a supporting platform 16 for placing experimental tools, and the supporting platform can be used for placing experimental tools such as stirring rods.
During the experiment, the mixing containers are sequentially and longitudinally arranged in the container groove, various liquids with different components required by the mixed liquid are respectively added into the pipettes, and the pipettes are respectively inserted into the insertion holes of the supporting seat; because the axes of the jacks are mutually intersected below the supporting seat, the curvature center of the cambered surface on the upper side of the supporting seat is superposed with the intersection points of the axes of the jacks, and the lower end of each pipette is converged above the mixing container; then, the pipette is sequentially controlled from left to right to perform liquid dropping according to the amount required by the experiment; after one group of liquid dripping is finished, the supporting rods on the two sides of the supporting seat are respectively held by two hands, the supporting rods are lifted up and then moved to a bayonet corresponding to the next mixing container, and liquid dripping is carried out according to the amount required by the second group of mixed liquid; and after the liquid dropping of the multiple groups of mixed liquid is completed in sequence, the supporting seat is moved to the position without the mixing container below, the stirring rod is taken down from the supporting platform, and the mixed liquid in each mixing container is stirred.
An experimental method using the experimental pipette rack described in this example for selecting preferred amounts of the components of the oral suspension of liposome-encapsulated lyophilized insulin formulations, comprising the steps of:
setting five comparison groups, selecting different preselected contents for each component of the mixed liquid, wherein the preselected contents of the same liquid in the different comparison groups have different values, marking the liquids of the components of the mixed liquid as liquid 1, liquid 2, liquid 3, liquid 4 and liquid 5 respectively, marking the comparison groups containing the liquids with different contents as group A, group B, group C, group D and group E, marking the content of the liquid 1 in the group A as a1, the content of the liquid 2 in the group A as a2, the content of the liquid 1 in the group B as B1, the content of the liquid 2 in the group B as B2 … … and so on, wherein the contents of the liquids of the components in the groups are different; in the embodiment, the mixed liquid is liposome-encapsulated insulin lyophilized preparation oral suspension, which needs the preferable components of lecithin solution, cholesterol solution, polyethylene glycol fatty acid ester solution, vitamin E solution and insulin solution, and the content of each solution in each control group is 2ml, 4ml, 6ml, 8ml and 10ml respectively.
Secondly, respectively sucking the liquid as each component of the mixed liquid into the pipettes, and respectively inserting the pipettes filled with different liquids into the insertion holes of the supporting seat.
And thirdly, 5 empty mixing containers are placed in the container groove, and the liquid of each component is dripped into the first mixing container according to the content of the liquid of each component in the group A.
Fourthly, the support seat is lifted and placed into the next bayonet, so that the lower end of each pipette is aligned to the second mixing container, and the liquid of each component is dripped into the second mixing container according to the content of the liquid of each component in the group B; the above procedure was repeated until all the controls were completed.
Fifthly, adding the solvent into each mixing container, stirring and mixing, and ensuring that the total amount of the liquid in each mixing container is 100ml after mixing.
Sixthly, measuring the liposome insulin encapsulation rate of each mixed liquid, and selecting the content of each component in the mixed liquid with the best detection result as the optimal content, wherein in the scheme, the content of each component in the mixed liquid with the best detection result is 6ml, and the content is the optimal content of each component in the mixed liquid.
Seventhly, selecting the mixed liquid with the best detection result as an X group, wherein the content of each component liquid is X1, the content of each component liquid is X2 … …, and the like, selecting the mixed liquid with the second best detection result as a Y group, the content of each component liquid is Y1, and the content of each component liquid is Y2 … …, and the like; in this example, the content of each component in the mixed liquid with the best detection result is 6ml, and the content of each component in the mixed liquid with the second best detection result is 4 ml.
Taking the content Y1 of the liquid 1 in the group Y as the content of the liquid 1, taking the content of other liquids except the liquid 1 in the group X as the content of other liquids, and recording the content as the group X1; recording the content Y2 of the liquid 2 in the group Y as the content of the liquid 2 and the content of the liquid other than the liquid 2 in the group X as the content of the other liquid as the group X2; … … and so on to form a new control group.
Ninthly, placing the empty mixing containers with the same number as the new control group into the container tank, and dripping the liquid of each component into the first mixing container according to the content of the liquid of each component in the X1 group.
R c then lifting the support base into the next bayonet, thereby aligning the lower end of each pipette with the second mixing vessel, dropping the liquid of each component into the second mixing vessel at the content of the liquid of each component in group X2; the above procedure was repeated until all new controls were completed.
⑪ adding solvent into each mixing container, stirring and mixing to ensure that the total amount of liquid in each mixing container after mixing is the same as the total amount of liquid in each mixing container in the fifth step.
⑫ performing target detection on each mixed liquid, selecting new group with detection result better than that of group X in control group, using the component content of group Y in these groups as the component content of the preferred group, using the other component content of the preferred group as the component content of the corresponding component in group X, and using the component content of each component in the preferred group as the preferred content correction value; in the scheme, the detection results of the X2 group and the X3 group are better than the detection results of the X group, so the preferable content correction value is as follows: 6ml of lecithin solution, 4ml of cholesterol solution, 4ml of polyethylene glycol fatty acid ester solution, 6ml of vitamin E solution and 6ml of insulin solution.
Claims (5)
1. An experimental method is characterized in that a pipette support is used for experiment, the pipette support comprises a support seat, a plurality of insertion holes for inserting pipettes are formed in the support seat, the axes of the insertion holes are intersected below the support seat, the upper side face of the support seat is an arc face gradually reducing in height from the middle to two sides, support rods are arranged on two sides of the support seat, the support rods are transversely erected on the support seat, a plurality of bayonets are arranged in the longitudinal direction of the support seat at intervals and are matched with the bayonets, the support seat comprises a base, a vertical strut and a longitudinal support seat, the bayonets are located on the longitudinal support seat, and a container groove for placing a container for mixing liquid is formed in the base; the method comprises the following steps when the bracket is used for carrying out experiments:
setting a plurality of control groups, selecting different preselected contents for each component of the mixed liquid, wherein the preselected contents of the same liquid in different control groups have different values, respectively marking the liquid of each component of the mixed liquid as liquid 1 and liquid 2 … …, and the like, marking the control groups containing the liquids with different contents as group A and group B … …, and the like, marking the content of the liquid 1 in the group A as a1, the content of the liquid 2 in the group A as a2, the content of the liquid 1 in the group B as B1, and the content of the liquid 2 in the group B as B2 … …, and the like;
respectively sucking the liquid serving as each component of the mixed liquid into a pipette, and respectively inserting the pipette filled with different liquids into the insertion holes of the supporting seat;
placing empty mixing containers with the same quantity as the reference group into the container groove, and dripping the liquid of each component into the first mixing container according to the content of the liquid of each component in the group A;
fourthly, the support seat is lifted and placed into the next bayonet, so that the lower end of each pipette is aligned to the second mixing container, and the liquid of each component is dripped into the second mixing container according to the content of the liquid of each component in the group B; repeating the above process until all the control groups have been added;
adding a solvent into each mixing container, stirring and mixing to ensure that the total amount of liquid in each mixing container is the same after mixing;
sixthly, carrying out target detection on the mixed liquid groups, and selecting the content of each component in the mixed liquid with the best detection result as the optimal content.
2. An experimental method according to claim 1, wherein the cross-section of the support rod and the cross-section of the bayonet are square.
3. An experimental method according to claim 1, wherein an arc-shaped sinking platform matched with the liquid storage part of the pipette is arranged on the upper side of the insertion hole.
4. An experimental method according to claim 1, wherein the base is provided with a supporting platform for holding experimental tools.
5. An experimental method according to claim 1, which further comprises the following steps after step (c):
seventhly, selecting the mixed liquid with the best detection result as an X group, wherein the content of each component liquid is X1, the content of each component liquid is X2 … …, and the like, selecting the mixed liquid with the second best detection result as a Y group, the content of each component liquid is Y1, and the content of each component liquid is Y2 … …, and the like;
taking the content Y1 of the liquid 1 in the group Y as the content of the liquid 1, taking the content of other liquids except the liquid 1 in the group X as the content of other liquids, and recording the content as the group X1; recording the content Y2 of the liquid 2 in the group Y as the content of the liquid 2 and the content of the liquid other than the liquid 2 in the group X as the content of the other liquid as the group X2; … … and so on to form a new control group;
ninthly, placing empty mixing containers with the same number as the new control group into the container tank, and dripping the liquid of each component into the first mixing container according to the content of the liquid of each component in the X1 group;
r c then lifting the support base into the next bayonet, thereby aligning the lower end of each pipette with the second mixing vessel, dropping the liquid of each component into the second mixing vessel at the content of the liquid of each component in group X2; repeating the above process until all new control groups have been added;
⑪ adding solvent into each mixing container, stirring and mixing to ensure that the total amount of liquid in each mixing container is the same as the total amount of liquid in each mixing container in the fifth step;
⑫ performing target detection on each mixed liquid, selecting new control group with detection result better than that of group X, using the component content of group Y as the component content of the preferred group, using the other component content of the preferred group as the component content of the corresponding component in group X, and using the component content of each component of the preferred group as the corrected value of the preferred content.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204987702U (en) * | 2015-09-11 | 2016-01-20 | 明博医药技术开发（上海）有限公司 | Round platform formula test tube dries frame |
| CN107661047A (en) * | 2017-11-20 | 2018-02-06 | 广东美的厨房电器制造有限公司 | Flavoring machine |
| CN108057389A (en) * | 2017-11-27 | 2018-05-22 | 北京莱伯泰科仪器股份有限公司 | Automatical dosing instrument |
| CN208679228U (en) * | 2018-03-23 | 2019-04-02 | 姜会萍 | A kind of adjustable angle rack for test tube |
| CN208839563U (en) * | 2018-10-08 | 2019-05-10 | 上海硕控电子科技有限公司 | A kind of liquid sample adding device |
| CN209810244U (en) * | 2019-04-01 | 2019-12-20 | 孙鹏 | Pharmacy test tube heating support |
| CN210097712U (en) * | 2019-05-24 | 2020-02-21 | 安永医疗科技常州有限公司 | Biomedical is with examining application of sample equipment just |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040094706A1 (en) * | 2001-04-09 | 2004-05-20 | Thomas Covey | Method of and apparatus for ionizing an analyte and ion source probe for use therewith |
-
2020
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Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204987702U (en) * | 2015-09-11 | 2016-01-20 | 明博医药技术开发（上海）有限公司 | Round platform formula test tube dries frame |
| CN107661047A (en) * | 2017-11-20 | 2018-02-06 | 广东美的厨房电器制造有限公司 | Flavoring machine |
| CN108057389A (en) * | 2017-11-27 | 2018-05-22 | 北京莱伯泰科仪器股份有限公司 | Automatical dosing instrument |
| CN208679228U (en) * | 2018-03-23 | 2019-04-02 | 姜会萍 | A kind of adjustable angle rack for test tube |
| CN208839563U (en) * | 2018-10-08 | 2019-05-10 | 上海硕控电子科技有限公司 | A kind of liquid sample adding device |
| CN209810244U (en) * | 2019-04-01 | 2019-12-20 | 孙鹏 | Pharmacy test tube heating support |
| CN210097712U (en) * | 2019-05-24 | 2020-02-21 | 安永医疗科技常州有限公司 | Biomedical is with examining application of sample equipment just |
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