CN111407720A

CN111407720A - Private part self-cleaning repair essence based on bioactive polysaccharide and active polypeptide

Info

Publication number: CN111407720A
Application number: CN202010359118.9A
Authority: CN
Inventors: 朱国君; 罗晓丹
Original assignee: Zhejiang Iseqi Pharmaceutical Technology Co ltd
Current assignee: Zhejiang Iseqi Pharmaceutical Technology Co ltd
Priority date: 2020-04-29
Filing date: 2020-04-29
Publication date: 2020-07-14
Also published as: CN111803436A

Abstract

The invention discloses a private part self-cleaning repair essence based on bioactive polysaccharide and active polypeptide, which is not added with thickening agent which can not be decomposed by human body such as carbomer resin, is thickened by natural glucan, can be subjected to 100% enzymolysis of glucosaccharase secreted by human body or bacteria in vivo, and has no residual accumulation risk, and the formula comprises 2-5% of glycerol, β -0.05-1% of glucan, 5-20% of pichia pastoris fermentation lysate filtrate, 0.5-2% of lysosome lysate, 0.1-1% of glucoside isoflavone, 0.4-0.8% of lactobacillus fermentation lysate, 0.1-1% of glycogen and the balance of water.

Description

Private part self-cleaning repair essence based on bioactive polysaccharide and active polypeptide

Technical Field

The invention relates to a health sanitary product, in particular to a private part self-cleaning repair essence based on bioactive polysaccharide and active polypeptide.

Background

In men and women, a large number of microorganisms including bacteria, fungi, viruses and the like exist in private parts of the men and women, the microorganisms have important effects on maintaining skin ecological balance, self-purification effect and participating in skin mucous membrane physiological functions, when the skin microecology is unbalanced, the microorganisms can become conditional pathogens or produce corresponding toxins to cause diseases, accumulated non-metabolizability caused by using conventional bactericides and antibiotics is disordered, normal cells of the skin are damaged, and an irrecoverable situation can be caused, scientific research faces more and more serious challenges, and inflammation is caused to be recurrent. Restoration and maintenance of normal microbial colonies is of great importance for maintaining skin health and treating problems associated with dysbacteriosis! Until the discovery of antibacterial polypeptide by human beings, the method brings about high attention and attention of biologists and medical circles, and the antibacterial peptide has strong selectivity to action objects (does not harm normal cells) and only has antibacterial action on prokaryotic cells and eukaryotic mutant cells (does not work on the normal cells of eukaryotes, human cells belong to the eukaryotic cells), can eliminate the mutant cells in vivo in natural immunity and is known as natural defense of the nature!

Numerous women talk about private topics and feel shy, but private care products have quietly become the necessary trend of numerous women, and the private care products on the market are full of precious and have various brands, so consumers tend to be careful by selecting the private care products, after all, the private care products are used on private parts, the safety is always the first place, and the female private parts are very fragile, and pathological phenomena can occur under the conditions of damage of external physical and chemical factors, invasion of pathogens, abnormal Hall and systemic diseases and the like. Privacy is just like the second face of a person, privacy care is more important than face care, and in daily life, the care consciousness of women in the private parts is not strong. Up to 91% of women suffer from private discomfort including unclean secretions, abnormal increased secretions, itching and rashes, itching, off-flavors, dry climacterium, and the like, and are particularly prone to discomfort during hot flashes, periods, climacterium, sedentary periods, and before and after intimacy. Compared with women, the male has weaker personal care consciousness. The lack of care in the private parts of men is the main reason for dissonance of husband and wife life after 30 years old, meanwhile, bacteria, scale, parasites and inflammation repeatedly invade the private parts of men, so that many men have phenomena of pruritus, peculiar smell, cross infection with partners and the like, and scientific research shows that many male health diseases such as urethritis, cystitis and the like are caused by invasion of external bacteria! The male cares the private parts without attention, which causes the infection of the private parts, the inflammation is frequent, and the reproductive health of the female is also directly influenced, which can cause the vaginitis of the female and even influence the fertility of the female. Privacy care for both men and women is equally important! Whether male or female, the daily lives are plagued by privacy problems and there is a lack of scientific knowledge in this regard.

At present, products which are disordered, low in quality and not long-term influence are enriched on the market, the effect is worse, flora imbalance, antibiotic abuse, repeated attack, local over-treatment and the like, and at present, medicines for hospitals comprise nystatin, dyclonine, metronidazole and the like, so that the traditional Chinese medicine has a certain curative effect, is very irritant to body, is allergic and has certain toxicity, and is forbidden for pregnant women, and is ineffective for other inflammations. The cation bactericide, the silver ion bactericide and the compound Chinese herbal medicine preparation which are commonly used in the word-removing type have cumulative damage and do not help to control the inflammation. Although private care products such as antibacterial peptide and estrogen supplement are used in the market, the effects are single, and the chemical synthesis and the biological activity are low. The cheap products in the market like adopting carbomer which is difficult to degrade by human bodies for thickening, increasing the burden and influencing the mucous membrane repairing effect.

Disclosure of Invention

In order to solve the defects and shortcomings in the prior art, the invention provides the private part self-cleaning repair essence based on the bioactive polysaccharide and the active polypeptide, which integrates pure nature and advanced biotechnology, has the advantages of bacteriostasis and sterilization, comfortable body feeling, definite effect, pure components, no hormone component, no chemical synthesis of the bacteriostasis and sterilization agent, no drug resistance, no stimulation to skin mucosa, greenness, safety and suitability for men and women, and is prepared by combining the pure nature and the advanced biotechnology.

The technical scheme includes that private self-cleaning repair essence based on bioactive polysaccharide and active polypeptide is prepared by the following formula, 2-5% of glycerol, 0.05-1% of β -glucan, 5-20% of pichia pastoris fermentation lysate filtrate, 0.5-2% of lysosome lysate, 0.1-1% of aglycone isoflavone, 0.4-0.8% of lactobacillus fermentation lysate, 0.1-1% of glycogen and the balance of water, wherein the natural glucan is thickened, 100% of glucosaccharase secreted by human bodies or bacteria in vivo can be subjected to enzymolysis, and no residue accumulation risk is generated.

Preferably, the high-activity biological polypeptide from the pichia pastoris fermentation lysate filtrate has the functions of resisting bacteria, improving immunity and cell regeneration, thereby achieving extremely strong repair capacity, the biological polypeptide can also directly or indirectly enhance the action of the inherent antibacterial peptide of the host, strengthen the innate immunity and epidemic prevention functions, has no stimulation or drug resistance, has no chemical action in the whole process, is prepared naturally, cannot cause damage to normal cells of a human body, has no toxic or side effect, can be decomposed by protease after entering the skin, the medicine can directly metabolize amino acid and water which are homologous to human bodies in vivo, can not accumulate subcutaneously, can repair mucous membrane regeneration, recover young state, improve the water wettability of female mucous membrane, promote the generation of vascular endothelial growth factor, and prevent xerosis of middle-aged and old women caused by vascular atrophy due to the change of self Hall.

Preferably, the high-activity biological polypeptide serving as a pichia pastoris fermentation source has a broad-spectrum antibacterial effect and has a strong bactericidal effect on common gram-positive bacteria, gram-negative bacteria and fungi.

Preferably, the yeast-derived lysosome lysate is a cell cleaner and transporter, promotes macrophage endocytosis, facilitates phagocytosis of P LL A microparticles, and has a large negative charge that can complex with the positive charge on the pathogen, resulting in a conformational change in the protein and inactivation.

Preferably, the secondary split yeast extract cell filtrate developed by the deep genetic fermentation engineering based on the submarine archaeological bacteria expression is rich in natural defense peptides, can regulate macrophage functions and regulate neurotransmitters, enhances the innate immunity function to resist bacterial infection and external harmful environment invasion, accelerates the metabolism of harmful substances, helps to discharge deposited harmful substances, prevents harmful bacteria from customizing and neutralizing endotoxin released by the harmful bacteria, reduces immunogenicity, further inhibits specific immune response, avoids deterioration, promotes the growth of VEGF, controls a vascular regeneration system, reorganizes the structure of a vascular network, and meanwhile, the cell filtrate is rich in a large amount of active amino acid, active polysaccharide and mineral substances, can accelerate the healing of micro wounds, prevents water loss, promotes the proliferation and differentiation of cells, and improves the healing force of mucous membranes.

Preferably, the aglucone isoflavone is selected from medicago sativa having higher biological activity than the glycoside form, and has effects of delaying climacteric aging, antioxidant, anti-inflammatory and moistening dryness, and simultaneously reducing hormone-dependent prostate inflammatory cell proliferation.

Preferably, glycogen serving as a biological fermentation source is decomposed into lactic acid under the action of lactobacillus, so that the normal acidic environment of the vagina is maintained, the natural defense function of a female is realized, pathogenic bacteria suitable for propagation in a weak alkaline environment are inhibited, and the private self-purification capacity is improved.

Preferably, the immunomodulator of the essence is derived from schizophyllan from fermentation of the white ginseng, is specially developed for regulating skin immunity, has a complete 3D three-dimensional triple-helix structure and a specific chemical conformation based on a liquid submerged fermentation technology and a modern bioseparation technology, can realize active transdermal absorption, can form effective cascade reaction with skin cell surface receptors only by a macromolecular triple-helix three-helix structure, and comprises phosphorylation of relevant proteins of an immunoregulation path, change of immune factor level and the like.

The bioactive polysaccharide synergistically synergizes two bioactive peptides and one aglucone isoflavone, promotes mucosal cell repair, inhibits inflammation, locally supplements and balances estrogen, resists dryness, comprehensively provides private nutrition, improves self-healing force, is not added with thickening agents which cannot be decomposed by a human body, such as carbomer resin, is thickened by organic glucan colloid, can be 100% enzymatically hydrolyzed by glucolase secreted by the human body or bacteria in vivo, and has no residual accumulation risk.

The invention combines pure nature and advanced biotechnology, and has the advantages of bacteriostasis and sterilization by biotechnology, comfortable body feeling, exact effect, pure components, no hormone component, no chemical synthesis of bacteriostasis and sterilization agent, no drug resistance, no stimulation to skin mucosa, greenness and safety, and suitability for men and women.

Drawings

FIG. 1 is a graph of the effect of the present invention against redness, stinging and itching;

FIG. 2 is a diagram showing the bacteriostatic effect of the high-activity biological polypeptide of the present invention;

FIG. 3 is a graph showing the bacteriostatic effect of lysosomal lysate of the present invention;

FIG. 4 is a graph showing the mechanism of inhibition of lysosomal lysate of the present invention.

Detailed Description

The present invention will be described in further detail with reference to the following drawings and examples, but the scope of the present invention is not limited thereto.

A self-cleaning repairing essence for private parts based on bioactive polysaccharide and active polypeptide is prepared from glycerin 2-5%, β -dextran (powder) 0.05-1%, Pichia pastoris fermented lysate filtrate 5-20%, lysosome lysate (yeast source) 0.5-2%, aglucone isoflavone 0.1-1%, lactobacillus lysed fermented product 0.4-0.8%, glycogen 0.1-1%, and water in balance.

White blood cell count results

Note: a indicates that the difference is not significant, and b indicates that the difference is significant

Counting result of bacteria in muscle tissue under wound surface

Note: a indicates that the difference is not significant, b indicates that the difference is significant, and c indicates that the difference is extremely significant

TABLE 1

The comparison of the effects of the privacy self-cleaning repairing group and the antibiotic resistance of the invention is shown in the table 1, and the effects are basically not different, but the antibiotic resistance is not existed.

The present invention is effective against redness, swelling, stinging and itching, the effect of which is shown in figure 1.

The polypeptide (high activity biological polypeptide) derived from pichia pastoris fermentation lysate filtrate is different from the traditional antibacterial peptide. The human skin is used as a first defense line for resisting the invasion of external pathogens, the host skin is actively and passively protected through multiple mechanisms, one important mechanism of the self-protection is the capability of the inherent secreted antibacterial peptide of the epidermis, the cells of the antibacterial peptide which can be secreted by the human skin are mainly keratinocytes, mast cells, neutrophils and sebaceous gland cells, and the bioactive peptide has a direct antibacterial function on the pathogens and can stimulate the release of inflammatory cells to snipe the nuclear cytokine. The bioactive polypeptide from Pichia pastoris fermentation has the functions of antibiosis, immunity improvement and cell regeneration, achieves extremely strong repair capacity, can directly or indirectly enhance the effect of the inherent antibacterial peptide of a host, and strengthens the inherent immunity and epidemic prevention function. The natural skin-care composition has the advantages of no stimulation and drug resistance, no chemical effect in the whole process, natural preparation, no harm to normal cells of a human body, no toxic or side effect, decomposition by protease after entering the skin, direct metabolism in vivo into homologous amino acid and water of the human body, no accumulation under the skin, regeneration by repairing mucous membrane, recovery of young state, improvement of the water wettability of female mucous membrane, promotion of the generation of vascular endothelial growth factor (shown in table 2), and prevention of xerosis of middle-aged and old women caused by the atrophy of blood vessels due to the change of self hall hormone. FIG. 2 shows the bacteriostatic effect of high-activity biological polypeptides. The polypeptide derived from the fermentation of the pichia pastoris has a broad-spectrum antibacterial effect and has a strong bactericidal effect on common gram-positive bacteria, gram-negative bacteria, fungi and the like.

Mouse VEGF expression level detection result

Note that a indicates no significant difference, b indicates significant difference, and c indicates very significant difference

TABLE 2

The lysosome lysate as a yeast source is a cleaning and handling tool of cells, promotes the endocytosis of macrophages, contributes to the phagocytosis of P LL A microparticles, has a large amount of negative charges, can be complexed with positive charges on pathogens, and leads to the change and inactivation of protein conformation (the bacteriostasis effect is shown in figure 3, and the bacteriostasis mechanism is shown in figure 4).

The secondary split yeast extract cell filtrate developed by the deep genetic fermentation engineering based on the submarine archaeological bacteria expression is rich in natural defense peptide, can regulate macrophage functions and neurotransmitter, enhances the inherent immune function, resists bacterial infection and invasion of external harmful environment, accelerates the metabolism of harmful substances, helps to discharge deposited harmful substances, prevents harmful bacteria from customizing and neutralizing endotoxin released by the harmful bacteria, reduces immunogenicity, further inhibits specific immune response, avoids deterioration, promotes the growth of VEGF, controls a vascular regeneration system, reorganizes the structure of a vascular network, and meanwhile, the cell filtrate is rich in a large amount of active amino acid, active polysaccharide and mineral substances, can accelerate the healing of micro wounds, prevents water loss, promotes the proliferation and differentiation of cells, and improves the healing force of mucous membranes.

Before and after menopause of women, due to hypoovarianism, in-vivo estrogen level is reduced, climacteric urogenital syndrome (GSM) appears, changes of vulva, vagina, urethra and bladder are involved, a series of diseases appear, and the purpose of preventing and treating the diseases can be achieved by supplementing estrogen. Symptoms may include sexual symptoms of genital dryness, burning, irritation, lubrication, and pain relief; and symptoms of urgency, dysuria and recurrent urinary tract infections. Unlike vasomotor symptoms that typically accompany menopause, GSM symptoms do not diminish over time and are less likely to resolve if left untreated. The symptoms and consequences of GSM can cause vulvovaginal discomfort for many years, with a significant impact on women's quality of life after menopause. Urogenital atrophy, despite its high incidence, is an under-reported and undiagnosed condition. Few women seek medical attention for vulvovaginal symptoms, often because they feel uncomfortable talking about this privacy problem. In recent years, phytoestrogen supplements have become more attractive as safer substitutes, natural phytoestrogen substitutes being more natural, safer and more effective, we have selected from lucerne, the aglucone isoflavones which are more biologically active to the body (more active than the glycoside form and chemically determined by the chemical structure. genistein is structurally similar to mammalian estrogen-estradiol with the active group of estrogen-dihydroxyl groups, so genistein has various physiological activities such as estrogenic-like activity), delaying climacteric aging, has antioxidant, antiinflammatory and dryness moistening effects, and also has certain effect in resisting male prostate diseases by reducing hormone-dependent prostate cell proliferation.

Glycogen: glycogen from biological fermentation is decomposed into lactic acid under the action of lactobacillus, so that the normal acidic environment of the vagina is maintained, the self natural defense function of a female is realized, pathogenic bacteria which are propagated in a weakly alkaline environment are inhibited, and the private self-purification capability is improved. (how much the self-cleaning effect is in fact.

The immunomodulator is derived from schizophyllan of a white ginseng bacterium fermentation source, is an innovative raw material specially developed for regulating skin immunity, and is based on a liquid submerged fermentation technology and a modern bioseparation technology. It has complete 3D double spiral structure and specific chemical conformation, and can realize active transdermal absorption. The macromolecular three-spiral 3D stereo structure can form effective cascade reaction with a skin cell surface receptor (mainly a Dectin-1 receptor), including phosphorylation of relevant proteins of an immunoregulation path, change of immune factor level and the like, the effective macromolecular membrane barrier is favorable for natural repair of tissues, self-defense capacity is improved, the macromolecular space stereo structure can protect and slowly release other active ingredients, synergistic effect is fully exerted, and effect is further guaranteed.

Examples

25 married women (older than 30 years, without hysterectomy, with 3 menopausal women, who did not use any study medication or exogenous hormones) were screened for follow-up for 2 months without discomfort during use of the product. And (4) investigation indexes are as follows: improvement in dryness, improvement in off-note pruritus inflammation (scores based on 4 points (0, none; 1, mild; 2, moderate; 3, severe) and follow-up of clinical use as shown in table 3.

Claims

1. A private part self-cleaning repair essence based on bioactive polysaccharide and active polypeptide is characterized in that a thickening agent which is not decomposed by human bodies, such as carbomer resin, is not added, natural glucan is adopted for thickening, 100% of glucosaccharase secreted by human bodies or bacteria in vivo can be enzymolyzed, and no residual accumulation risk exists, and the private part self-cleaning repair essence is prepared from 2-5% of glycerol, 0.05-1% of β -glucan, 5-20% of Pichia pastoris fermentation lysate filtrate, 0.5-2% of lysosome lysate, 0.1-1% of aglycone isoflavone, 0.4-0.8% of lactobacillus fermentation lysate, 0.1-1% of glycogen and the balance of water.

2. The essence of claim 1, wherein the essence comprises a bioactive polysaccharide and a bioactive polypeptide, wherein the essence comprises: the high-activity biological polypeptide from pichia pastoris fermentation lysate filtrate has the functions of resisting bacteria, improving immunity and cell regeneration, and thus has extremely strong repair capacity, can also directly or indirectly enhance the effect of host inherent antibacterial peptide, enhances the inherent immunity epidemic prevention function, has no stimulation and drug resistance, has no chemical effect in the whole process, is prepared naturally, cannot cause damage to normal cells of a human body, has no toxic or side effect, can be decomposed by protease after entering the skin, can be directly metabolized into human body homologous amino acid and water in vivo, cannot be accumulated subcutaneously, can repair mucosal regeneration at the same time, recover the young state, improve the water wettability of female mucosa, promote the generation of vascular endothelial growth factors, and prevent the xerosis of the middle-aged and old women caused by the atrophy of blood vessels due to the change of self-hormone.

3. The essence according to claim 2, wherein the essence comprises a bioactive polysaccharide and a bioactive polypeptide, wherein the essence comprises: the high-activity biological polypeptide serving as a fermentation source of the pichia pastoris has a broad-spectrum antibacterial effect and has a strong bactericidal effect on common gram-positive bacteria, gram-negative bacteria and fungi.

4. The essence of claim 1, wherein the yeast-derived lysosome lysate is selected from the group consisting of cell cleaner and transporter, macrophage endocytosis, and P LL A microparticle phagocytosis, and the lysosome lysate has a large negative charge that can complex with the positive charge of the pathogen, resulting in a conformational change in the protein and inactivation.

5. The essence of claim 4, wherein the essence comprises a bioactive polysaccharide and a bioactive polypeptide, wherein the essence comprises: the secondary split yeast extract cell filtrate developed by the deep genetic fermentation engineering based on the submarine archaeological bacteria expression is rich in natural defense peptide, can regulate macrophage functions and neurotransmitter, enhances the inherent immune function, resists bacterial infection and invasion of external harmful environment, accelerates the metabolism of harmful substances, helps to discharge deposited harmful substances, prevents harmful bacteria from customizing and neutralizing endotoxin released by the harmful bacteria, reduces immunogenicity, further inhibits specific immune response, avoids deterioration, promotes the growth of VEGF, controls a vascular regeneration system, reorganizes the structure of a vascular network, and meanwhile, the cell filtrate is rich in a large amount of active amino acid, active polysaccharide and mineral substances, can accelerate the healing of micro wounds, prevents water loss, promotes the proliferation and differentiation of cells, and improves the healing force of mucous membranes.

6. The essence according to claim 1, wherein the essence is characterized in that: wherein the aglucone isoflavone is selected from Medicago sativa having a higher biological activity than the glycoside form, and has effects in delaying climacteric aging, resisting oxidation, relieving inflammation and moistening dryness, and reducing hormone-dependent prostatic inflammatory cell proliferation.

7. The essence according to claim 1, wherein the essence is characterized in that: glycogen serving as a biological fermentation source is decomposed into lactic acid under the action of lactobacillus, so that the normal acidic environment of the vagina is maintained, the natural defense function of a female is realized, pathogenic bacteria suitable for propagation in a weakly alkaline environment are inhibited, and the private self-purification capacity is improved.

8. The essence according to claim 1, wherein the essence is characterized in that: the immunomodulator of the essence is derived from schizophyllan from a white ginseng bacterium fermentation source, is specially developed for adjusting skin immunity, has a complete 3D three-dimensional triple-helix structure and a specific chemical conformation based on a liquid submerged fermentation technology and a modern bioseparation technology, can realize active transdermal absorption, can form effective cascade reaction with skin cell surface receptors only by the three-helix structure of macromolecules, and comprises phosphorylation of relevant proteins of an immunoregulation channel, change of immune factor level and the like, an effective macromolecular membrane barrier is beneficial to natural repair of tissues, the self-defense capability is improved, other slow-release active ingredients can be protected by the macromolecular spatial three-helix structure, the synergistic effect is fully exerted, and the effect is further ensured.