CN111407453A - Integrated blood collecting and perfusion device for small animals - Google Patents

Abstract

The invention discloses a small animal integrated blood collecting and perfusion device, which comprises a collecting pipe and a puncture indwelling device, wherein the puncture indwelling device is provided with a collecting needle and a perfusion pipe which are matched and combined, so that continuous blood collecting and perfusion operation can be realized, the device does not need to be repeatedly replaced in the process, the operation is convenient and fast, the death rate of small animals before perfusion is greatly reduced, and the perfusion effect is ensured; in addition, the vacuum pumping treatment is carried out in the tube body of the collecting tube, so that the contact between blood and air is reduced, the purity of the collected blood is improved, and after the tail end of the collecting needle is inserted into the tube body from the cap, the blood in the small animal body automatically flows into the tube body under the action of pressure, so that the blood collecting efficiency is improved better.

Description

Integrated blood collecting and perfusion device for small animals

Technical Field

The invention relates to the field of biological experiment instruments, in particular to a small animal integrated blood collecting and perfusion device.

Background

In the experimental research of biological science, blood collection and whole body perfusion fixation of experimental animals are of great importance. Through blood collection, researchers can analyze the concentrations of substances such as cell components, medicines, hormones, molecular compounds and the like in blood of different experimental animals, so that the inherent pathophysiological changes and mechanisms in the experimental animal models are understood and elucidated. The whole body perfusion fixation through a vascular system is an important processing means for obtaining and storing tissue and organ specimens of experimental animals, and is the basis for carrying out subsequent researches on histology, pathology, morphology and the like.

At present, in common means, an experimental animal instrument capable of integrally collecting blood and perfusing is lacked, a plurality of independent operating tools are manually manufactured, and the using effect is limited. There are the following problems: the device is suitable for single animals, poor in fixity, easy to slide and remove pipes, easy to block pipes, high in death rate before animal perfusion, influenced in perfusion effect, poor in sealing performance, easy to leak, and harmful to safety of experimenters, and the like, and blood sampling perfusion needs repeated operation in multiple times.

Disclosure of Invention

The invention aims to solve at least one of the technical problems in the prior art and provides a small animal integrated blood collecting and perfusion device which combines the functions of blood collection and perfusion and is simple and convenient to operate.

The small animal integrated blood collecting and perfusion apparatus comprises a collecting pipe and a puncture indwelling device, wherein the collecting pipe is provided with a pipe body with an opening and a cap which is arranged on the pipe body to seal the opening, and the inside of the pipe body is vacuumized; the puncture indwelling device comprises a collecting needle, a perfusion tube sleeved outside the collecting needle, a combining part communicated with the bottom end of the perfusion tube and a liquid inlet tube with one end communicated with the combining part, wherein the combining part is of a hollow structure; the tail end of the collecting needle penetrates through the combining part and is inserted into the cap to enter the tube body.

The small animal integrated blood collecting and perfusion apparatus provided by the embodiment of the invention at least has the following beneficial effects: the integrated blood collection and perfusion device for the small animals comprises a collection pipe and a puncture indwelling device, wherein the puncture indwelling device is provided with a collection needle and a perfusion pipe which are matched and combined, so that continuous blood collection and perfusion operations can be realized, the devices do not need to be replaced repeatedly in the process, the operation is convenient and fast, the death rate of the small animals before perfusion is greatly reduced, and the perfusion effect is ensured; moreover, the vacuum pumping treatment is carried out inside the tube body of the collecting tube, so that the contact between blood and air is reduced, the purity of the collected blood is improved, and after the tail end of the collecting needle is inserted into the tube body from the cap, the blood in the small animal body automatically flows into the tube body under the action of pressure, so that the blood collecting efficiency is improved better.

According to some embodiments of the present invention, the top end and the bottom end of the collecting needle form an inclined plane respectively, a conical cover is disposed at a position of the collecting needle close to the bottom end, a widening direction of the conical cover points to the top end of the collecting needle, and the conical cover and the tail end of the collecting needle are wrapped and sealed by the same sealing film.

According to some embodiments of the invention, the perfusion tube comprises a tube head and a tube body connected below the tube head, the tube head is in a shuttle shape, and the upper section of the tube body is provided with a plurality of protruding parts around the outer wall.

According to some embodiments of the present invention, each of the protruding members has a shape of a lobe with a tip, an included angle is formed between each of the protruding members and an outer wall of the tube body, and the tip of each of the protruding members points to the bottom of the tube body.

According to some embodiments of the invention, the outer wall of the tube body is marked with at least one scale mark, and each scale mark extends downwards from the top of the tube body along the axial direction of the tube body.

According to some embodiments of the invention, the liquid inlet pipe is provided with a liquid stopping device, and the other end of the liquid inlet pipe is connected with a three-way joint which is communicated with an external perfusate supply device.

According to some embodiments of the invention, a through hole for the collection needle to be clamped is formed in the middle of the top of the cap, and the through hole is covered with a sealing membrane.

According to some embodiments of the invention, the cap is threadably connected to the body.

According to some embodiments of the invention, a handle is provided on the joint.

According to some embodiments of the invention, the bottom of the combining portion forms a gap for the tail end of the collecting needle to pass through, and an isolation plug is detachably arranged at the gap.

Drawings

The invention will be further described with reference to the accompanying drawings in which:

FIG. 1 is a schematic diagram showing the separation of the collection tube and the puncture indwelling device of the small-animal integrated blood collection and perfusion apparatus according to the embodiment of the present invention;

FIG. 2 is a block diagram of an acquisition needle in accordance with an embodiment of the present invention;

FIG. 3 is a schematic view of a connection structure of the perfusion tube, the joint part and the liquid inlet tube according to the embodiment of the present invention;

fig. 4 is a schematic structural view of a cap of an embodiment of the present invention.

Detailed Description

Reference will now be made in detail to the present preferred embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to like elements throughout.

In the description of the present invention, it should be understood that the orientation or positional relationship referred to in the description of the orientation, such as the upper, lower, front, rear, left, right, etc., is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

In the description of the present invention, the meaning of a plurality of means is one or more, the meaning of a plurality of means is two or more, and larger, smaller, larger, etc. are understood as excluding the number, and larger, smaller, inner, etc. are understood as including the number. If the first and second are described for the purpose of distinguishing technical features, they are not to be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.

In the description of the present invention, unless otherwise explicitly limited, terms such as arrangement, installation, connection and the like should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above terms in the present invention in combination with the specific contents of the technical solutions.

Referring to fig. 1 to 4, the small animal integrated blood collecting and perfusion apparatus according to the embodiment of the present invention includes a collection tube 1 and a puncture indwelling device 2 which are combined together.

Specifically, the collection tube 1 includes a tube body 11 having an opening and a cap 12 mounted on the tube body 11 to close the opening, and the interior of the tube body 11 is evacuated; the puncture indwelling device 2 comprises a collecting needle 21, a perfusion tube 22 sleeved outside the collecting needle 21, a combining part 23 communicated with the bottom end of the perfusion tube 22 and a liquid inlet tube 24 with one end communicated to the combining part 23, wherein the combining part 23 is of a hollow structure; the tail end of the collecting needle 21 passes through the combining part 23 and is inserted into the cap 12 to enter the tube body 11.

The small animal integrated blood collecting and perfusion device comprises a collecting pipe 1 and a puncture indwelling device 2, wherein the puncture indwelling device 2 is provided with a collecting needle 21 and a perfusion pipe 22 which are combined in a matched mode, continuous blood collecting and perfusion operations can be realized, devices do not need to be replaced repeatedly in the process, the operation is convenient and fast, the death rate of small animals before perfusion is greatly reduced, and the perfusion effect is ensured; moreover, the interior of the tube body 11 of the collecting tube 1 is vacuumized, and after the tail end of the collecting needle 21 is inserted into the tube body 11 from the cap 12, blood in the small animal body automatically flows into the tube body 11 under the action of pressure, so that the blood collecting efficiency is improved better.

In some embodiments of the present invention, referring to fig. 2, the top end and the bottom end of the collecting needle 21 are respectively formed with an inclined surface, a conical cover 211 is disposed at a position of the collecting needle 21 close to the bottom end, the widening direction of the conical cover 211 points to the top end of the collecting needle 21, and the conical cover 211 and the tail end of the collecting needle 21 are wrapped and sealed by the same sealing film 3. The top end is set to be in an inclined plane instead of a plane, so that the opening area of the collecting needle 21 is increased, the blood can enter the collecting needle 21 more conveniently, and the blood collecting efficiency is effectively accelerated; further, in this embodiment, the sealing film 3 is made of rubber. When the tail end of the collection needle 21 enters the cap 12, the sealing film 3 is retained outside the tube body 11 under the blocking of the cone-shaped cover 211 and the cap 12, so that the influence on the normal blood collection caused by the blockage of the bottom end of the collection needle 21 is avoided. In order to improve the corrosion resistance of the collecting needle 21 while ensuring the structural strength thereof, the collecting needle 21 of the present embodiment is made of stainless steel.

More specifically, as shown in fig. 3, the perfusion tube 22 comprises a tube head 221 and a tube body 222 connected below the tube head 221, wherein the tube head 221 is disposed in a shuttle shape in order to effectively prevent the perfusion tube 22 from deforming during puncturing. Meanwhile, the upper section of the tube body 222 is provided with a plurality of protruding members 2221 around the outer wall, and the protruding members 2221 are uniformly distributed around the outer wall; the provision of projection 2221 herein improves the grasping force between perfusion tube 22 and the organs or tissues of the small animal, effectively preventing the occurrence of a situation where perfusion tube 22 backs up or slips off. Further, in the present embodiment, each of the protruding members 2221 has a shape of a lobe with a tip, and each of the protruding members 2221 forms an included angle with the outer wall of the tube body 222, and the tip points to the bottom of the tube body 222. Preferably, in order to accurately select the insertion depth of the perfusion tube 22 according to the animal species and size, at least one scale mark 2222 is marked on the outer wall of the tube body 222 of the perfusion tube 22, and each scale mark 2222 extends downwards from the top of the tube body 222 along the axial direction of the tube body 222; it should be noted that the distribution form of the scales on each scale mark 2222 may be the same as the marks, or may be different.

In this embodiment, the material of perfusion tube 22 is polytetrafluoroethylene, and the perfusion tube 22 of utilizing polytetrafluoroethylene to make, the wall is thin and the wall is smooth, and the resistance is little, can reduce the platelet and adsorb the gathering, and the condition that reduces stifled pipe takes place.

In some embodiments of the present invention, the liquid stopping device 4 is installed on the liquid inlet pipe 24, and the other end of the liquid inlet pipe 24 is connected with a three-way joint 5 which is communicated with an external perfusate supplying device. In the pouring process, the liquid stopping device 4 is operated, so that the pouring can be stopped temporarily, and the pouring progress can be adjusted according to the condition. Referring to fig. 1, the liquid inlet pipe 24 is a transparent hollow hose made of polyvinyl chloride plastic; the liquid stopping device 4 is a temporary pipe sealing clamp clamped on the liquid inlet pipe 24. The transparent liquid inlet pipe 24 can enable experimenters to observe the entering of the perfusion liquid, thereby timely and rapidly adjusting the perfusion process.

In some embodiments of the present invention, as shown in fig. 4, a through hole for the collection needle 21 to be inserted is formed at a top middle position of the cap 12, and the through hole is covered with a cover film 121, specifically, the cover film 121 includes a plurality of rubber films laminated in sequence. Due to the arrangement, the situation of air leakage at the through hole after the cap 12 and the tube body 11 are combined is avoided while the tail end of the collecting needle 21 is conveniently pierced. More specifically, threaded connection between the cap 12 and the tube 11, an internal thread is formed on the inner wall of the side wall member of the cap 12, and an external thread matching the internal thread is provided on the outer wall of the upper portion of the tube 11. In order to better identify different collection tubes 1, the outer wall of the tube body 11 of each collection tube 1 is provided with an information marking area 111.

Furthermore, different kinds of pretreatment solutions, such as a coagulant, an inert separation gel, a coagulant, heparin, a heparin lithium anticoagulant, ethylenediaminetetraacetic acid, sodium citrate, etc., may be reserved in the tube 11 to protect blood components and facilitate subsequent experimental analysis.

In some embodiments of the present invention, the bottom of the combining portion 23 forms a notch for the tail end of the collecting needle 21 to pass through, and a isolating plug 231 is detachably disposed at the notch, specifically, in order to ensure the sealing effect, the isolating plug is a rubber plug. When the collection needle 21 falls off the infusion tube 22 and the joint part 23 (i.e. after being pulled out), the bottom gap of the joint part 23 can be sealed by using the isolating plug, so that a closed cavity structure is formed inside the joint part 23, and the situation that the infusion liquid leaks from the gap in the infusion process is avoided. More specifically, the joint portion 23 is provided with a handle 6 to facilitate holding of the puncture indwelling device 2 by an experimenter. Further, the combining part 23 is made of polymethyl methacrylate, is colorless and transparent, and is arranged in such a way that the operation process of the whole filling process is visualized.

The integrated blood collecting and perfusion device for the small animals is suitable for various common big and small mice for life science experiments, specifically comprises a Sprague-Dawley (SD) rat, a Wistar rat, a L ewis rat, a C57B L mouse, a BA L B/C mouse, a BA L B/C nude mouse, a KM mouse and the like, and is favorable for popularization.

Taking an experimental SD rat as an example, the conventional heart blood sampling and perfusion operation is carried out, and the specific steps are as follows:

a. selecting a proper collecting pipe 1, respectively connecting the frozen normal saline and a 4% paraformaldehyde perfusate injector to two interfaces of a three-way joint 5, and closing a temporary pipe sealing clamp;

b. rats were anesthetized routinely, the chest was dissected, and the cardiac structures were exposed;

c. the handle 6 is held by hand, the acquisition needle 21 and the perfusion tube 22 penetrate into the left ventricle along the apex of the heart of the rat, and the scale on the scale mark 2222 is observed to ensure that the embedding depth of the perfusion tube 22 is 4 mm;

d. inserting the tail end of the collecting needle 21 into the cap 12, collecting blood of the rat, directly pulling out the collecting tube 1 to enable the collecting needle 21 to withdraw from the heart of the rat after the blood is collected, and placing the collecting tube 1 in an environment of 4 ℃ for preservation;

e. opening the temporary sealing pipe clamp, cutting the right auricle of the rat, and simultaneously injecting frozen normal saline through the three-way joint 5;

f. after the liver of the rat becomes white or the flow liquid of the auricle is clarified, injecting needed amount of 4% paraformaldehyde through a three-way joint 5, dissecting and collecting needed tissues after the whole body of the rat is fixed, and soaking the tissues in the 4% paraformaldehyde to store in a refrigerator environment at 4 ℃;

g. the cap 12 of the collecting tube 1 is replaced, after the relevant information is marked in the information marking area, the collecting tube 1 is placed in a refrigerator environment at 4 ℃ for storage, and the collecting needle 21 is recovered in a sharp box.

The invention is not limited to the above embodiments, and those skilled in the art can make equivalent modifications or substitutions without departing from the spirit of the invention, and such equivalent modifications or substitutions are included in the scope of the claims of the present application.

Claims (10)

1. Little animal integral type blood is gathered and perfusion ware, its characterized in that includes:

a collection tube having a tube body with an opening, and a cap attached to the tube body to close the opening, the tube body being evacuated;

the puncture indwelling device comprises a collecting needle, a perfusion tube sleeved outside the collecting needle, a combining part communicated with the bottom end of the perfusion tube and a liquid inlet tube with one end communicated with the combining part, wherein the combining part is of a hollow structure;

the tail end of the collecting needle penetrates through the combining part and is inserted into the cap to enter the tube body.

2. The small-animal integrated blood collection and perfusion apparatus of claim 1, wherein: gather the top of needle, the bottom forms an inclined plane respectively, it disposes the toper cover to gather on the needle is close to the position of bottom, the directional top of gathering the needle of the widening direction of toper cover, the toper cover is airtight through same seal membrane parcel with the tail end of gathering the needle.

3. The small-animal integrated blood collection and perfusion apparatus of claim 1, wherein: the filling pipe comprises a pipe head and a pipe body connected below the pipe head, the pipe head is in a shuttle shape, and a plurality of protruding parts are arranged on the upper section of the pipe body around the outer wall.

4. The small-animal integrated blood collection and perfusion apparatus of claim 3, wherein: the shape of each protruding piece is the lobe form that possesses the pointed end, and each protruding piece forms the contained angle respectively with the outer wall of pipe shaft between, the pointed end of each protruding piece points to the bottom in the pipe shaft.

5. The small-animal integrated blood collection and perfusion apparatus of claim 3, wherein: the outer wall of pipe shaft is drawn and is equipped with at least one scale mark, each the scale mark all extends downwards along the axial of pipe shaft from the top of pipe shaft.

6. The small-animal integrated blood collection and perfusion apparatus of claim 1, wherein: the liquid inlet pipe is provided with a liquid stopping device, the other end of the liquid inlet pipe is connected with a three-way joint, and the three-way joint is communicated with an external perfusate supply device.

7. The small-animal integrated blood collection and perfusion apparatus of claim 1, wherein: a through hole for clamping the collecting needle is formed in the middle of the top of the cover cap, and a sealing membrane is covered at the through hole.

8. The small-animal integrated blood collection and perfusion apparatus of claim 1, wherein: the cap is in threaded connection with the pipe body.

9. The small-animal integrated blood collection and perfusion apparatus of claim 1, wherein: the combining part is provided with a handle.

10. The small-animal integrated blood collection and perfusion apparatus of claim 1, wherein: the bottom of the combining part forms a gap for the tail end of the collecting needle to pass through, and the gap is detachably provided with an isolating plug.

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