CN111394233A

CN111394233A - Cell inspection is with high-efficient breaker

Info

Publication number: CN111394233A
Application number: CN202010252217.7A
Authority: CN
Inventors: 李容尔
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-04-01
Filing date: 2020-04-01
Publication date: 2020-07-10

Abstract

The invention discloses a high-efficiency crushing device for cell inspection, which relates to the technical field of cell crushing and comprises a support frame, wherein a plurality of pairs of crushing blades are fixed on the surface of a hollow shaft at equal intervals; according to the cell crushing device, cells in the crushing groove can be cut and crushed in a rotating mode through the arranged crushing mechanism, the rotating shaft rotates along with the rotating rack, the transmission gear is meshed with the inner gear ring to achieve autorotation, so that the crushing blades on the rotating shaft can fully cut and crush the cells in the crushing groove in the circumferential direction, the arranged reversing mechanism can drive the lifting mechanism to do vertical reciprocating motion, namely the piston plate can push the cells in the crushing groove to vertically move up and down relative to the crushing mechanism, the crushing effect of the cells in the crushing groove in the vertical direction is greatly improved, and the cell crushing efficiency is remarkably improved.

Description

Cell inspection is with high-efficient breaker

Technical Field

The invention relates to the technical field of cell disruption, in particular to a high-efficiency disruption device for cell inspection.

Background

Cells are the basic structural and functional units of an organism. It is known that all organisms except viruses are composed of cells, but viral life activities must be expressed in cells. Generally, most microorganisms such as bacteria and protozoa are composed of one cell, i.e., unicellular organisms, and higher plants and higher animals are multicellular organisms. The cells can be divided into two types of prokaryotic cells and eukaryotic cells, but the division into three types is also proposed, namely ancient nuclear cells originally belonging to the prokaryotic cells are independently taken as the parallel type. The discipline of studying cells is called cell biology.

Application publication number is CN 109022276A's patent discloses a high-efficient cell disruption device, belongs to cell disruption technical field, including base and box, install box, display screen, set up button, landing leg and P L C controller on the base, supersonic generator, magnetic stirring device and fixed column are installed to the box, fixed column internally mounted has the slide bar rather than sliding connection, the bearing is installed to the slide bar bottom, install the bracing piece in the bearing, the magneton is installed to the bracing piece bottom, and the device can carry out the breakage to the cell and refine, but the abundant degree of breakage of cell is lower in the operation process, and the cell in the container can not obtain comprehensive crushing effect, and for this, provides a high-efficient disruption device for cell inspection now to solve above-mentioned technical problem.

Disclosure of Invention

The present invention is directed to a high-efficiency cell disruption device for cell examination, which solves the above-mentioned problems of the background art.

In order to achieve the purpose, the invention provides the following technical scheme:

the utility model provides a cell inspection is with high-efficient breaker, includes the support frame, and the vertical broken groove that is fixed with in the support frame, support frame top are fixed with driving motor I, and driving motor I's output shaft is connected with through drive mechanism and is used for carrying out broken mechanism to broken inslot cell, broken mechanism is fixed with a plurality ofly to broken blade including rotating the hollow shaft that sets up, hollow shaft surface equidistant, the support frame bottom is fixed with driving motor II, and driving motor II's output shaft is connected with elevating system through reversing mechanism, and the last transmission of elevating system is connected with the piston board with the sealed sliding connection of driving motor, be equipped with the aeration mechanism who is used for to broken inslot portion air-blowing.

As a modified scheme of the invention: the transmission mechanism comprises a driven bevel gear which is sleeved and fixed on the hollow shaft, and a driving bevel gear which is coaxially fixed with the driving motor I is connected to the driven bevel gear in a meshed mode.

As a modified scheme of the invention: the crushing mechanism further comprises a rotating frame fixed on the hollow shaft in a sleeved mode, a vertically arranged rotating shaft is pivoted on the rotating frame, and a plurality of pairs of crushing blades are uniformly and fixedly mounted on the side wall of the rotating shaft at equal intervals.

As a modified scheme of the invention: an inner gear ring sleeved outside the rotating frame is fixed at the inner top of the supporting frame, and a transmission gear meshed and connected with the inner gear ring is sleeved and fixed on the rotating shaft.

As a modified scheme of the invention: elevating system includes coaxial fixed and screw rod that the screw thread revolves to opposite, and the screw rod cover piece has been cup jointed to the screw thread on the screw rod, and piston plate bottom is fixed with the bottom plate, and bottom plate bottom slidable mounting has the carriage, and it has the bracing piece to articulate between carriage and the screw rod cover piece.

As a modified scheme of the invention: the bottom plate lateral wall level has seted up the spout, and sliding fit is equipped with the slider of fixing at the carriage inner wall in the spout, is fixed with the spring that is located the spout between slider and the bottom plate.

As a modified scheme of the invention: a guide rod is horizontally fixed on the support frame, and a sliding sleeve fixed at the bottom of the screw rod sleeve block is sleeved on the guide rod in a sliding manner.

As a modified scheme of the invention: the reversing mechanism comprises an incomplete bevel gear coaxially fixed with an output shaft of the driving motor II, a bevel gear I and a bevel gear II which are respectively positioned at two sides of the incomplete bevel gear are fixedly sleeved on the screw rod, and the incomplete bevel gear is alternately meshed with the bevel gear I and the bevel gear II.

As a modified scheme of the invention: the aeration mechanism comprises an air pump fixed at the top of the support frame, the outlet end of the air pump is connected with an air pipe, the end part of the air pipe is communicated with the hollow shaft through a rotary joint, and the lower end of the air pipe is provided with an air nozzle.

As a modified scheme of the invention: and a delivery pump is fixed on the support frame, and the inlet end of the delivery pump is communicated with the crushing groove through a corrugated pipe.

Compared with the prior art, the invention has the beneficial effects that:

according to the cell crushing device, cells in the crushing groove can be cut and crushed in a rotating mode through the arranged crushing mechanism, the rotating shaft rotates along with the rotating rack, the transmission gear is meshed with the inner gear ring to achieve autorotation, so that the crushing blades on the rotating shaft can fully cut and crush the cells in the crushing groove in the circumferential direction, the arranged reversing mechanism can drive the lifting mechanism to do vertical reciprocating motion, namely the piston plate can push the cells in the crushing groove to vertically move up and down relative to the crushing mechanism, the crushing effect of the cells in the crushing groove in the vertical direction is greatly improved, and the cell crushing efficiency is remarkably improved.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is an enlarged view of portion A of FIG. 1;

FIG. 3 is an enlarged view of portion B of FIG. 1;

FIG. 4 is a schematic partial top view of the present invention;

fig. 5 is a partial perspective view of the present invention.

In the figure: 1-supporting frame, 2-crushing groove, 3-air pump, 4-air pipe, 5-driven bevel gear, 6-hollow shaft, 7-driving bevel gear, 8-driving motor I, 9-crushing blade, 10-delivery pump, 11-corrugated pipe, 12-slide block, 13-air nozzle, 14-rotating shaft, 15-piston plate and 16-bottom plate, 17-screw rod, 18-screw rod sleeve block, 19-support rod, 20-incomplete bevel gear, 21-drive motor II, 22-bevel gear I, 23-guide rod, 24-sliding sleeve, 25-bevel gear II, 26-spring, 27-sliding groove, 28-sliding frame, 29-support rod, 30-rotating frame, 31-inner gear ring and 32-transmission gear.

Detailed Description

The technical scheme of the patent is further described in detail by combining the following specific embodiments:

example 1

Referring to fig. 1-5, a high-efficiency crushing device for cell examination comprises a support frame 1, a crushing tank 2 is vertically fixed in the support frame 1, a driving motor I8 is fixed at the top of the support frame 1, an output shaft of the driving motor I8 is connected with a crushing mechanism for crushing cells in the crushing tank 2 through a transmission mechanism, the crushing mechanism comprises a hollow shaft 6 which is rotatably arranged, a plurality of pairs of crushing blades 9 are fixed on the surface of the hollow shaft 6 at equal intervals, a driving motor 21 is fixed at the bottom of the support frame 1, an output shaft of the driving motor 21 is connected with a lifting mechanism through a reversing mechanism, a piston plate 15 which is in sealing sliding connection with the driving motor 21 is in transmission connection with the lifting mechanism, and an aeration mechanism for blowing air to the inside of the crushing tank 2 is arranged on the.

The inside cell that will carry out the breakage and refine that is used for loading of broken groove 2 that sets up in this device, carries out rotatory breakage to the cell in the broken groove 2 through the crushing mechanism that sets up, and the drive mechanism of this device establishes driven bevel gear 5 on hollow shaft 6 including the cover, and the meshing is connected with the drive bevel gear 7 with the coaxial fixed of driving motor 8 on the driven bevel gear 5.

The driving motor I8 drives the driving bevel gear 7 to rotate, the driving bevel gear 7 drives the hollow shaft 6 to rotate through the driven bevel gear 5 meshed with the driving bevel gear, and the hollow shaft 6 drives the crushing blade 9 on the hollow shaft to rotate so as to realize the crushing effect on cells.

The crushing mechanism further comprises a rotating frame 30 which is fixedly sleeved on the hollow shaft 6, a vertically arranged rotating shaft 14 is pivoted on the rotating frame 30, a plurality of pairs of crushing blades 9 are uniformly and fixedly arranged on the side wall of the rotating shaft 14 at equal intervals, an inner gear ring 31 which is sleeved outside the rotating frame 30 is fixed at the top in the supporting frame 1, and a transmission gear 32 which is meshed with the inner gear ring 31 is fixedly sleeved on the rotating shaft 14.

In the process of rotating the hollow shaft 6, the hollow shaft 6 drives the rotating frame 30 to rotate, the rotating frame 30 drives the rotating shaft 14 on the rotating frame to rotate around the hollow shaft 6, meanwhile, the transmission gear 32 on the rotating shaft 14 is in meshing transmission with the inner gear ring 31, and then the rotation of the rotating shaft 14 is realized, namely the rotating shaft 14 drives the crushing blade 9 on the rotating shaft to rotate around the hollow shaft 6 at the same time, and the sufficient and efficient crushing effect of the crushing blade 9 on the cells in the crushing tank 2 is realized.

Example 2

On the basis of embodiment 1, in addition, the lifting mechanism provided by the device comprises a coaxially fixed screw rod 17 with opposite thread turning directions, a screw rod sleeve block 18 is sleeved on the screw rod 17 in a threaded manner, a bottom plate 16 is fixed at the bottom of a piston plate 15, a sliding frame 28 is slidably mounted at the bottom of the bottom plate 16, and a support rod 29 is hinged between the sliding frame 28 and the screw rod sleeve block 18.

When the two screw rods 17 rotate, the screw rod sleeve blocks 18 on the screw rods 17 move oppositely or backwards, the screw rod sleeve blocks 18 drive the sliding frame 28 through the screw rod sleeve blocks 18 to realize the pushing effect on the bottom plate 16, at the moment, the bottom plate 16 can drive the piston plate 15 to vertically slide and lift relative to the crushing groove 2, and the crushing mechanism can fully crush and refine cells at different vertical positions in the crushing groove 2.

Furthermore, a sliding groove 27 is horizontally formed in the side wall of the bottom plate 16, a sliding block 12 fixed on the inner wall of the sliding frame 28 is embedded in the sliding groove 27 in a sliding manner, a spring 26 located in the sliding groove 27 is fixed between the sliding block 12 and the bottom plate 16, a guide rod 23 is horizontally fixed on the support frame 1, and a sliding sleeve 24 fixed at the bottom of the lead screw sleeve block 18 is sleeved on the guide rod 23 in a sliding manner.

When the sliding frame 28 slides, the sliding block 12 on the sliding frame 28 slides along the sliding groove 27, the sliding block 12 pushes the spring 26 to compress or extend, in the process that the spring 26 compresses or extends to a stable length, the vertical position of the piston plate 15 is unchanged, the crushing mechanism can fully crush cells at a certain vertical position, and meanwhile, the compression or extension of the spring 26 improves the lifting smoothness of the piston plate 15.

The reversing mechanism of the device comprises an incomplete bevel gear 20 coaxially fixed with an output shaft of a driving motor II21, a bevel gear I22 and a bevel gear II25 which are respectively positioned at two sides of the incomplete bevel gear 20 are fixedly sleeved on the screw rod 17, and the incomplete bevel gear 20 is alternately in meshed connection with the bevel gear I22 and the bevel gear II 25.

The driving motor II21 drives the incomplete bevel gear 20 to rotate, the incomplete bevel gear 20 is alternately meshed with the bevel gear I22 and the bevel gear II25, and further the forward and reverse rotation alternate rotation of the screw rod 17 is realized, namely the screw rod sleeve blocks 18 move oppositely or backwards, the reciprocating lifting of the piston plate 15 is realized, and the effect of moving the vertical position of the cells in the crushing tank 2 is achieved.

Further, the aeration mechanism is including fixing the air pump 3 at 1 top of support frame, and the exit end of air pump 3 is connected with gas-supply pipe 4, and 4 tip of gas-supply pipe pass through rotary joint and 6 intercommunication settings of hollow shaft, and air nozzle 13 is installed to 4 lower extremes of gas-supply pipe, is fixed with delivery pump 10 on the support frame 1, and the entrance point of delivery pump 10 passes through

bellows

11 and 2 intercommunications in broken groove.

The pressurized air that air pump 3 produced is injected into hollow shaft 6 through gas-supply pipe 4 in, and the air is finally through the dispersion blowout of jet 13, realizes the effect of blowing air to the inside of broken groove 2, promotes turning of cell, promotes broken mechanism and to the broken degree of filling of cell, and the cell breakage back that finishes, delivery pump 10 can extract the transport with broken cell in the broken groove 2 through bellows 11.

In conclusion, the cell crushing device can rotationally cut and crush cells in the crushing tank 2 through the arranged crushing mechanism, the arranged rotating shaft 14 rotates along with the rotating frame 30, the transmission gear 32 is meshed with the inner gear ring 31 to achieve autorotation, the crushing blade 9 on the rotating shaft 14 can fully cut and crush the cells in the crushing tank 2 in the circumferential direction, the arranged reversing mechanism can drive the lifting mechanism to vertically reciprocate, namely, the piston plate 15 can push the cells in the crushing tank 2 to vertically move up and down relative to the crushing mechanism, the crushing effect of the cells in the crushing tank 2 in the vertical direction is greatly improved, and the cell crushing efficiency is remarkably improved.

It should be noted that, although the present specification describes embodiments, each embodiment does not include only a single technical solution, and such description of the specification is only for clarity, and those skilled in the art should take the specification as a whole, and the technical solutions in the embodiments may be appropriately combined to form other embodiments understood by those skilled in the art, and the above-mentioned embodiments only express the preferred embodiments of the technical solutions, and the description thereof is more specific and detailed, but should not be construed as limiting the scope of the claims of the technical solutions. It should be noted that, for those skilled in the art, various changes, modifications and substitutions can be made without departing from the spirit of the present invention, and these changes and substitutions belong to the protection scope of the present invention. The protection scope of this technical solution patent should be subject to the appended claims.

Claims

1. An efficient crushing device for cell examination comprises a support frame (1), a crushing groove (2) is vertically fixed in the support frame (1), it is characterized in that a driving motor (8) I is fixed at the top of the supporting frame (1), an output shaft of the driving motor I (8) is connected with a crushing mechanism for crushing cells in the crushing tank (2) through a transmission mechanism, the crushing mechanism comprises a hollow shaft (6) which is arranged in a rotating way, a plurality of pairs of crushing blades (9) are fixed on the surface of the hollow shaft (6) at equal intervals, a driving motor (21) is fixed at the bottom of the support frame (1), an output shaft of the driving motor II (21) is connected with a lifting mechanism through a reversing mechanism, a piston plate (15) which is in sealing sliding connection with the driving motor II (21) is connected on the lifting mechanism in a transmission way, and an aeration mechanism for blowing air into the crushing tank (2) is arranged on the support frame (1).

2. The efficient disruption apparatus for cell assay as claimed in claim 1, wherein said transmission mechanism comprises a driven bevel gear (5) fixed on the hollow shaft (6), and a driving bevel gear (7) coaxially fixed with the driving motor I (8) is engaged with the driven bevel gear (5).

3. The efficient crushing device for cell inspection according to claim 1, wherein the crushing mechanism further comprises a rotating frame (30) sleeved and fixed on the hollow shaft (6), the rotating frame (30) is pivoted with a vertically arranged rotating shaft (14), and a plurality of pairs of crushing blades (9) are uniformly and fixedly arranged on the side wall of the rotating shaft (14) at equal intervals.

4. The efficient crushing device for cell inspection according to claim 3, wherein an inner gear ring (31) sleeved outside the rotating frame (30) is fixed at the inner top of the support frame (1), and a transmission gear (32) engaged and connected with the inner gear ring (31) is sleeved and fixed on the rotating shaft (14).

5. The efficient crushing device for cell inspection according to claim 1, wherein the lifting mechanism comprises a screw rod (17) which is coaxially fixed and has opposite thread turning directions, a screw rod sleeve block (18) is sleeved on the screw rod (17) in a threaded manner, a bottom plate (16) is fixed at the bottom of the piston plate (15), a sliding frame (28) is slidably mounted at the bottom of the bottom plate (16), and a support rod (29) is hinged between the sliding frame (28) and the screw rod sleeve block (18).

6. The efficient cell disruption device for cell assay as claimed in claim 5, wherein a sliding groove (27) is horizontally formed on the side wall of the bottom plate (16), a sliding block (12) fixed on the inner wall of the sliding frame (28) is slidably inserted into the sliding groove (27), and a spring (26) located in the sliding groove (27) is fixed between the sliding block (12) and the bottom plate (16).

7. The efficient cell disruption device for cell inspection according to claim 5, wherein a guide rod (23) is horizontally fixed on the support frame (1), and a sliding sleeve (24) fixed at the bottom of the screw rod sleeve block (18) is slidably sleeved on the guide rod (23).

8. The efficient crushing device for cell inspection according to claim 5, wherein the reversing mechanism comprises an incomplete bevel gear (20) coaxially fixed with an output shaft of the driving motor II (21), a lead screw (17) is sleeved and fixed with a bevel gear I (22) and a bevel gear II (25) respectively positioned at two sides of the incomplete bevel gear (20), and the incomplete bevel gear (20) is alternately meshed and connected with the bevel gear I (22) and the bevel gear II (25).

9. The efficient crushing device for cell examination according to claim 1, wherein the aeration mechanism comprises an air pump (3) fixed at the top of the support frame (1), an air pipe (4) is connected to the outlet end of the air pump (3), the end part of the air pipe (4) is communicated with the hollow shaft (6) through a rotary joint, and an air nozzle (13) is installed at the lower end of the air pipe (4).

10. The efficient disruption apparatus for cell assay as claimed in claim 1, wherein a transfer pump (10) is fixed to said support frame (1), and an inlet end of said transfer pump (10) is connected to said disruption tank (2) via a bellows (11).