CN111393489A - Purification and separation method of N-acetyl-D-glucosamine - Google Patents
Purification and separation method of N-acetyl-D-glucosamine Download PDFInfo
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- CN111393489A CN111393489A CN202010382677.1A CN202010382677A CN111393489A CN 111393489 A CN111393489 A CN 111393489A CN 202010382677 A CN202010382677 A CN 202010382677A CN 111393489 A CN111393489 A CN 111393489A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D36/00—Filter circuits or combinations of filters with other separating devices
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H13/00—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids
- C07H13/02—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids
- C07H13/04—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids having the esterifying carboxyl radicals attached to acyclic carbon atoms
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Abstract
The invention discloses a method for purifying and separating N-acetyl-D-glucosamine, and an extraction and separation device is used in the purification and separation process, the device comprises a working bin, a partition plate is fixed in the working bin, the upper surface of the partition plate is matched with the interior of the working bin to form a stirring bin, a discharging pipe is fixed in the center of the lower surface of the partition plate, a material conveying pipe is fixed on one side of the discharging pipe, a liquid collecting bin is fixed at the bottom end of the discharging pipe, a second motor is fixed on the outer side wall of the working bin, a screw rod is fixed at the output end of the second motor, a material scattering barrel is arranged on the screw rod, and a sieve plate is fixed in the working bin; the arrangement of the rotating plate enables the filter to be fully contacted with hot air, and the filter continuously moves on the material sieving plate, so that the dried filter rapidly passes through the material sieving plate to be separated from the filter which is not dried, repeated drying of the filter is further prevented, and the work efficiency of purification and separation of the N-acetyl-D-glucosamine is further improved.
Description
Technical Field
The invention belongs to the technical field of biochemistry, and particularly relates to a method for purifying and separating N-acetyl-D-glucosamine.
Background
N-acetyl-D-glucosamine is a novel biochemical drug, is a composition unit of various polysaccharides in organisms, particularly has the highest exoskeleton content in crustaceans, is a medicament for clinically treating rheumatic and rheumatoid arthritis, can also be used as a food antioxidant, an infant food additive and a sweetener for diabetics, is mainly used for clinically enhancing the function of a human immune system, inhibiting the excessive growth of cancer cells or fiber cells, has the effects of inhibiting and treating cancers and malignant tumors, can effectively treat various inflammations and also has the effect of treating osteoarthritis and arthralgia.
The traditional purification and separation method of the N-acetyl-D-glucosamine requires various processes such as suction filtration and drying of raw materials, and requires various devices, so that the purification and separation cost of the N-acetyl-D-glucosamine is too high, and in the drying process, partial raw materials are dried and still dried continuously, and the work efficiency of purification and separation of the N-acetyl-D-glucosamine is too low.
Disclosure of Invention
The invention aims to provide a method for purifying and separating N-acetyl-D-glucosamine.
The technical problems to be solved by the invention are as follows:
the traditional purification and separation method of N-acetyl-D-glucosamine requires various processes such as suction filtration and drying of raw materials, and requires various devices for the processes, so that the purification and separation cost of the N-acetyl-D-glucosamine is too high, and in the drying process, part of raw materials are dried and still dried, so that the work efficiency of the purification and separation of the N-acetyl-D-glucosamine is too low.
The purpose of the invention can be realized by the following technical scheme:
a method for purifying and separating N-acetyl-D-glucosamine specifically comprises the following steps:
step S1: adding chitin and hydrochloric acid solution into a reaction kettle, stirring until the chitin is dissolved under the condition that the rotating speed is 300-;
step S2: adding the D-glucosamine hydrochloride prepared in the step S1, acetic anhydride, triethylamine and a hydrochloric acid solution into a reaction kettle, and reacting for 2-3h at the temperature of 15-20 ℃ to prepare a mixed solution containing N-acetyl-D-glucosamine;
step S3: concentrating the mixed solution prepared in the step S2 until the mixed solution reaches a supersaturated state to obtain a concentrated solution, adding the concentrated solution and an ethanol solution into a stirring kettle, stirring until the concentrated solution and the ethanol solution are uniformly stirred, and centrifuging to obtain a crude product of the N-acetyl-D-glucosamine;
step S4: adding the N-acetyl-D-glucosamine crude product prepared in the step S3 and deionized water into a stirring bin through a feeding hole, starting a first motor, driving a stirring shaft to rotate by the first motor, driving stirring blades to rotate by taking the stirring shaft as an axis by the stirring shaft, stirring for 30-40min under the condition that the rotation speed is 1000r/min, starting a valve in a blanking pipe, exhausting air from an exhaust pipe, performing suction filtration through a filter plate, starting a valve in the conveying pipe, enabling filtered substances to fall into a material scattering barrel through the conveying pipe, starting a second motor and a valve in a material scattering port, driving a screw to rotate by the second motor to enable the material scattering barrel to move, scattering the filtered substances on a sieve plate, starting a third motor, driving a rotating shaft to rotate, driving a conical block to rotate, driving a rotating plate to rotate by the conical block to enable the filtered substances to be uniformly distributed on the sieve plate, blowing hot air out from the blast head, drying the filtered substances at the temperature of 100-110 ℃, and collecting the filtered substances from a discharge port to obtain the pure N-acetyl-D-glucosamine.
Further, the mass ratio of the chitin to the hydrochloric acid solution in the step S1 is 1:6, the mass fraction of the hydrochloric acid solution is 35-40%, and the mass ratio of the D-glucosamine hydrochloride, acetic anhydride, triethylamine, and the hydrochloric acid solution in the step S2 is 2: 1: 1: 0.05, 20-25% of hydrochloric acid solution, 95% of ethanol solution in the step S3, and the mass ratio of the N-acetyl-D-glucosamine crude product in the step S4 to deionized water is 1: 4.
further, the extraction and separation device of step S4 includes a working bin, a feeding port is disposed at the top end of the working bin, a discharging port is disposed at the bottom end of the working bin, a partition board is fixed inside the working bin, an upper surface of the partition board cooperates with the inside of the working bin to form a stirring bin, a first motor is fixed at the top end of the outside of the stirring bin, a stirring shaft is fixed at the output end of the first motor, the stirring shaft is located inside the stirring bin, the stirring shaft is rotatably connected with the top wall of the stirring bin, a plurality of uniformly distributed stirring blades are fixed on the stirring shaft, a discharging pipe is fixed at the center of the lower surface of the partition board, the discharging pipe is communicated with the inside of the stirring bin, a filter plate is fixed inside the discharging pipe, a feeding pipe is fixed at one side of the discharging pipe, the feeding pipe is communicated with the inside of the discharging pipe, a liquid collecting bin is fixed at the bottom, one side of collecting the liquid storehouse is equipped with the exhaust tube, the end of exhaust tube is located the outside of working bin, the outside lateral wall of working bin is fixed with the second motor, the output of second motor is fixed with the screw rod, the screw rod is located the inside of working bin, the screw rod is located the below of collecting the liquid storehouse, the screw rod rotates with the lateral wall of working bin to be connected, be equipped with on the screw rod and spill the storage bucket, spill the storage bucket and be located the conveying pipeline under, the inside of working bin is fixed with the sieve flitch, the sieve flitch is located the below of spilling the storage bucket.
Further, the below of working bin be equipped with the base, be fixed with four evenly distributed's fixed column between base and the working bin, the inside of working bin is equipped with a plurality of evenly distributed's blast head, the blast head is located spills between storage bucket and the sieve flitch.
Furthermore, a valve is arranged in the joint of the blanking pipe and the partition plate, the filter plate forms an angle of 30 degrees with the horizontal plane, the conveying pipe is positioned at the lower end of the filter plate, and the bottom end of the joint of the conveying pipe and the blanking pipe and the lower end of the filter plate are positioned on the same horizontal plane.
Further, spill the bottom of storage bucket be equipped with spill the material mouth, spill the inside of material mouth and be equipped with the valve, spill the top of storage bucket and be the open end, spill the top of storage bucket and be fixed with the connecting block that two opposition set up, connecting block and screw rod looks spiro union spill the lateral wall of storage bucket and be fixed with the connecting rod, the inside of working bin is fixed with the horizontal pole, the horizontal pole is located same height with the screw rod, the horizontal pole parallels with the screw rod, the connecting rod is mutually perpendicular with the horizontal pole, connecting rod and horizontal pole sliding connection.
Further, the lower surface center department of sieve flitch be fixed with the third motor, the output of third motor is fixed with the pivot, the top of pivot is fixed with the toper piece, the lower surface of toper piece and the upper surface of sieve flitch are laminated mutually, the pivot rotates with the sieve flitch to be connected, toper piece both sides are fixed with changes the board, the lower surface of changeing the board is laminated mutually with the upper surface of sieve flitch.
The invention has the beneficial effects that: the invention drives a stirring shaft to rotate through a first motor, the stirring shaft drives stirring blades to rotate by taking the stirring shaft as an axis, stirring is carried out, a valve in a blanking pipe is opened, air is exhausted from an exhaust pipe, suction filtration is carried out through a filter plate, filtrate can be collected through the arrangement of a liquid collecting bin, the pollution of the filtrate to the environment is prevented, the valve in a conveying pipe is opened, the filtrate falls into a material scattering barrel through the conveying pipe, the valve in a second motor and a material scattering port is opened, the second motor drives a screw rod to rotate so as to enable the material scattering barrel to move, the filtrate is scattered on a sieve plate, a third motor is started, the third motor drives a rotating shaft to rotate, the rotating shaft drives a conical block to rotate, the conical block drives a rotating plate to rotate, the filtrate is uniformly distributed on the sieve plate, hot air is blown out through a blast head, the filtrate is dried, and the rotating plate is arranged so that the filtrate can be fully, further, the drying rate of the filtered substances is improved, the filtered substances are continuously moved on the material sieving plate, the dried filtered substances are enabled to rapidly penetrate through the material sieving plate to be separated from the dried filtered substances which are not finished, further, repeated drying of the filtered substances is prevented, further, the working efficiency of purification and separation of the N-acetyl-D-glucosamine is improved, equipment required by a multi-step purification and separation process of the N-acetyl-D-glucosamine is integrated, and the cost of purification and separation is greatly reduced.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural diagram of an extraction and separation device in the purification and separation method of N-acetyl-D-glucosamine of the present invention;
FIG. 2 is a top view of the inner part of the material spreading barrel and the working bin of the extraction and separation device of the present invention;
FIG. 3 is a schematic view of a portion of the structure of FIG. 1;
fig. 4 is a top view of a screen plate in the extraction separation device of the present invention.
In the figure: 1. a working bin; 11. a feed inlet; 12. a discharge port; 13. a base; 14. fixing a column; 15. a partition plate; 151. a discharging pipe; 152. filtering the plate; 153. a delivery pipe; 16. a blowing head; 2. a stirring bin; 21. a first motor; 22. a stirring shaft; 23. a stirring blade; 3. a liquid collecting bin; 31. an air exhaust pipe; 4. a second motor; 41. a screw; 42. a material scattering barrel; 421. connecting blocks; 422. a connecting rod; 43. a cross bar; 5. a material sieving plate; 51. a third motor; 52. a rotating shaft; 53. a conical block; 54. and (6) rotating the plate.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A method for purifying and separating N-acetyl-D-glucosamine specifically comprises the following steps:
step S1: adding chitin and hydrochloric acid solution into a reaction kettle, stirring until the chitin is dissolved under the condition that the rotating speed is 300r/min, heating until the reaction solution is boiled under the condition that the temperature is 55 ℃, and carrying out reflux reaction for 3.5 hours to prepare D-glucosamine hydrochloride;
step S2: adding the D-glucosamine hydrochloride prepared in the step S1, acetic anhydride, triethylamine and a hydrochloric acid solution into a reaction kettle, and reacting for 3 hours at the temperature of 15 ℃ to prepare a mixed solution containing N-acetyl-D-glucosamine;
step S3: concentrating the mixed solution prepared in the step S2 until the mixed solution reaches a supersaturated state to obtain a concentrated solution, adding the concentrated solution and an ethanol solution into a stirring kettle, stirring until the concentrated solution and the ethanol solution are uniformly stirred, and centrifuging to obtain a crude product of the N-acetyl-D-glucosamine;
step S4: and (4) adding the N-acetyl-D-glucosamine crude product prepared in the step (S3) and deionized water into an extraction and separation device to prepare the pure N-acetyl-D-glucosamine.
Example 2
A method for purifying and separating N-acetyl-D-glucosamine specifically comprises the following steps:
step S1: adding chitin and hydrochloric acid solution into a reaction kettle, stirring until the chitin is dissolved under the condition that the rotating speed is 500r/min, heating until the reaction solution is boiled under the condition that the temperature is 60 ℃, and carrying out reflux reaction for 3 hours to prepare D-glucosamine hydrochloride;
step S2: adding the D-glucosamine hydrochloride prepared in the step S1, acetic anhydride, triethylamine and a hydrochloric acid solution into a reaction kettle, and reacting for 2 hours at the temperature of 20 ℃ to prepare a mixed solution containing N-acetyl-D-glucosamine;
step S3: concentrating the mixed solution prepared in the step S2 until the mixed solution reaches a supersaturated state to obtain a concentrated solution, adding the concentrated solution and an ethanol solution into a stirring kettle, stirring until the concentrated solution and the ethanol solution are uniformly stirred, and centrifuging to obtain a crude product of the N-acetyl-D-glucosamine;
step S4: and (4) adding the N-acetyl-D-glucosamine crude product prepared in the step (S3) and deionized water into an extraction and separation device to prepare the pure N-acetyl-D-glucosamine.
Referring to fig. 1-4, the extraction and separation device according to the above embodiment includes a working bin 1, a feeding port 11 is disposed at a top end of the working bin 1, a discharging port 12 is disposed at a bottom end of the working bin 1, a partition 15 is fixed inside the working bin 1, an upper surface of the partition 15 is matched with an inside of the working bin 1 to form a stirring bin 2, a first motor 21 is fixed at an outer top end of the stirring bin 2, a stirring shaft 22 is fixed at an output end of the first motor 21, the stirring shaft 22 is located inside the stirring bin 2, the stirring shaft 22 is rotatably connected with a top wall of the stirring bin 2, a plurality of uniformly distributed stirring blades 23 are fixed on the stirring shaft 22, a discharging pipe 151 is fixed at a center of a lower surface of the partition 15, the discharging pipe 151 is communicated with an inside of the stirring bin 2, a filter plate 152 is fixed inside the discharging pipe 151, a feeding pipe 153 is fixed at one side of the discharging pipe, the bottom mounting of unloading pipe 151 has a liquid collecting bin 3, unloading pipe 151 and the inside intercommunication in liquid collecting bin 3, one side and the lateral wall fixed connection of working bin 1 in liquid collecting bin 3, one side in liquid collecting bin 3 is equipped with exhaust tube 31, the end of exhaust tube 31 is located the outside of working bin 1, the outside lateral wall of working bin 1 is fixed with second motor 4, the output of second motor 4 is fixed with screw rod 41, screw rod 41 is located the inside of working bin 1, screw rod 41 is located the below in liquid collecting bin 3, screw rod 41 rotates with the lateral wall of working bin 1 to be connected, be equipped with on the screw rod 41 and spill storage bucket 42, spill storage bucket 42 is located the conveying pipeline 153 under, the inside of working bin 1 is fixed with sieve material board 5, sieve material board 5 is located the below of spilling storage bucket 42.
A base 13 is arranged below the working bin 1, four fixing columns 14 which are uniformly distributed are fixed between the base 13 and the working bin 1, a plurality of uniformly distributed blast heads 16 are arranged inside the working bin 1, and the blast heads 16 are positioned between the material scattering barrel 42 and the material sieving plate 5.
A valve is arranged in the joint of the blanking pipe 151 and the partition 15, the filter plate 152 forms an angle of 30 degrees with the horizontal plane, the feed delivery pipe 153 is positioned at the lower end of the filter plate 152, and the bottom end of the joint of the feed delivery pipe 153 and the blanking pipe 151 is positioned at the same horizontal plane with the lower end of the filter plate 152.
The lower surface center department of sieve flitch 5 be fixed with third motor 51, the output of third motor 51 is fixed with pivot 52, the top of pivot 52 is fixed with toper piece 53, the lower surface of toper piece 53 and the laminating of the upper surface of sieve flitch 5 mutually, pivot 52 rotates with sieve flitch 5 to be connected, toper piece 53 both sides are fixed with changes board 54, the lower surface of changing board 54 and the laminating of the upper surface of sieve flitch 5 mutually.
The working process and the working principle of the extraction and separation device are as follows:
adding the N-acetyl-D-glucosamine crude product prepared in the step S3 and deionized water into the stirring bin 2 through the feeding hole 11, starting the first motor 21, driving the stirring shaft 22 to rotate by the first motor 21, driving the stirring blades 23 to rotate by taking the stirring shaft 22 as the axis, stirring for 30-40min under the condition that the rotation speed is 800-, the third motor 51 is started, the third motor 51 drives the rotating shaft 52 to rotate, the rotating shaft 52 drives the conical block 53 to rotate, the conical block 53 drives the rotating plate 54 to rotate, so that the filtered matters are uniformly distributed on the sieve plate 5, hot air is blown out from the blast head 16, the filtered matters are dried at the temperature of 100 ℃ plus 110 ℃, the rotating plate 54 is arranged so that the filtered matters can be fully contacted with the hot air, the drying rate of the filtered matters is further improved, the filtered matters continuously move on the sieve plate 5, the dried filtered matters rapidly penetrate through the sieve plate 5, the repeated drying of the filtered matters is further prevented, and the pure N-acetyl-D-glucosamine is obtained by collecting through the discharge port 12.
The foregoing is merely exemplary and illustrative of the principles of the present invention and various modifications, additions and substitutions of the specific embodiments described herein may be made by those skilled in the art without departing from the principles of the present invention or exceeding the scope of the claims set forth herein.
Claims (8)
1. A method for purifying and separating N-acetyl-D-glucosamine is characterized by comprising the following steps: the method specifically comprises the following steps:
step S1: adding chitin and hydrochloric acid solution into a reaction kettle, stirring until the chitin is dissolved under the condition that the rotating speed is 300-;
step S2: adding the D-glucosamine hydrochloride prepared in the step S1, acetic anhydride, triethylamine and a hydrochloric acid solution into a reaction kettle, and reacting for 2-3h at the temperature of 15-20 ℃ to prepare a mixed solution containing N-acetyl-D-glucosamine;
step S3: concentrating the mixed solution prepared in the step S2 until the mixed solution reaches a supersaturated state to obtain a concentrated solution, adding the concentrated solution and an ethanol solution into a stirring kettle, stirring until the concentrated solution and the ethanol solution are uniformly stirred, and centrifuging to obtain a crude product of the N-acetyl-D-glucosamine;
step S4: and (4) adding the N-acetyl-D-glucosamine crude product prepared in the step (S3) and deionized water into an extraction and separation device to prepare the pure N-acetyl-D-glucosamine.
2. The method for purifying and separating N-acetyl-D-glucosamine according to claim 1, wherein the method comprises the following steps: the mass ratio of the chitin to the hydrochloric acid solution in the step S1 is 1:6, the mass fraction of the hydrochloric acid solution is 35-40%, and the mass ratio of the D-glucosamine hydrochloride, the acetic anhydride, the triethylamine and the hydrochloric acid solution in the step S2 is 2: 1: 1: 0.05, 20-25% of hydrochloric acid solution, 95% of ethanol solution in the step S3, and the mass ratio of the N-acetyl-D-glucosamine crude product in the step S4 to deionized water is 1: 4.
3. the method for purifying and separating N-acetyl-D-glucosamine according to claim 1, wherein the method comprises the following steps: the extraction and separation device comprises a working bin (1), a feed inlet (11) is formed in the top end of the working bin (1), a discharge outlet (12) is formed in the bottom end of the working bin (1), a partition plate (15) is fixed inside the working bin (1), the upper surface of the partition plate (15) is matched with the inside of the working bin (1) to form a stirring bin (2), a first motor (21) is fixed at the top end of the outside of the stirring bin (2), a stirring shaft (22) is fixed at the output end of the first motor (21), the stirring shaft (22) is located inside the stirring bin (2), the stirring shaft (22) is rotatably connected with the top wall of the stirring bin (2), a plurality of uniformly distributed stirring blades (23) are fixed on the stirring shaft (22), a blanking pipe (151) is fixed at the center of the lower surface of the partition plate (15), the blanking pipe (151) is communicated with the inside of the stirring bin (2), a filter plate (152) is fixed inside the blanking, a feed delivery pipe (153) is fixed on one side of the feed delivery pipe (151), the feed delivery pipe (153) is communicated with the interior of the feed delivery pipe (151), a liquid collecting bin (3) is fixed at the bottom end of the feed delivery pipe (151), the feed delivery pipe (151) is communicated with the interior of the liquid collecting bin (3), one side of the liquid collecting bin (3) is fixedly connected with the side wall of the working bin (1), an air suction pipe (31) is arranged on one side of the liquid collecting bin (3), the end head of the air suction pipe (31) is positioned outside the working bin (1), a second motor (4) is fixed on the outer side wall of the working bin (1), a screw rod (41) is fixed at the output end of the second motor (4), the screw rod (41) is positioned inside the working bin (1), the screw rod (41) is positioned below the liquid collecting bin (3), the screw rod (41) is rotatably connected with the side wall of the working bin (1), a material scattering barrel (42) is arranged on the screw rod (41), and the material, a material sieving plate (5) is fixed in the working bin (1), and the material sieving plate (5) is positioned below the material scattering barrel (42).
4. The method for purifying and separating N-acetyl-D-glucosamine according to claim 3, wherein the method comprises the following steps: the material scattering and screening device is characterized in that a base (13) is arranged below the working bin (1), four fixing columns (14) which are uniformly distributed are fixed between the base (13) and the working bin (1), a plurality of blowing heads (16) which are uniformly distributed are arranged inside the working bin (1), and the blowing heads (16) are located between the material scattering barrel (42) and the material screening plate (5).
5. The method for purifying and separating N-acetyl-D-glucosamine according to claim 3, wherein the method comprises the following steps: the filter is characterized in that a valve is arranged inside the joint of the discharging pipe (151) and the partition plate (15), the filter plate (152) and the horizontal plane form an angle of 30 degrees, the conveying pipe (153) is positioned at the lower end of the filter plate (152), and the bottom end of the joint of the conveying pipe (153) and the discharging pipe (151) and the lower end of the filter plate (152) are positioned on the same horizontal plane.
6. The method for purifying and separating N-acetyl-D-glucosamine according to claim 3, wherein the method comprises the following steps: spill the bottom of storage bucket (42) be equipped with and spill the material mouth, the inside of spilling the material mouth is equipped with the valve, the top of spilling storage bucket (42) is the open end, the top of spilling storage bucket (42) is fixed with connecting block (421) that two opposition set up, connecting block (421) and screw rod (41) looks spiro union, the lateral wall of spilling storage bucket (42) is fixed with connecting rod (422), the inside of working bin (1) is fixed with horizontal pole (43), horizontal pole (43) and screw rod (41) are located same height, horizontal pole (43) and screw rod (41) parallel, connecting rod (422) are mutually perpendicular with horizontal pole (43), connecting rod (422) and horizontal pole (43) sliding connection.
7. The method for purifying and separating N-acetyl-D-glucosamine according to claim 3, wherein the method comprises the following steps: the lower surface center department of sieve flitch (5) be fixed with third motor (51), the output of third motor (51) is fixed with pivot (52), the top of pivot (52) is fixed with toper piece (53), the lower surface of toper piece (53) is laminated with the upper surface of sieve flitch (5) mutually, pivot (52) rotate with sieve flitch (5) and are connected, toper piece (53) both sides are fixed with and change board (54), the lower surface of changeing board (54) is laminated with the upper surface of sieve flitch (5) mutually.
8. The method for purifying and separating N-acetyl-D-glucosamine according to claim 1, wherein the method comprises the following steps: the specific steps of step S4 are as follows:
adding the N-acetyl-D-glucosamine crude product prepared in the step S3 and deionized water into a stirring bin (2) from a feeding hole (11), starting a first motor (21), driving a stirring shaft (22) to rotate by the first motor (21), driving a stirring blade (23) to rotate by taking the stirring shaft (22) as an axis by the stirring shaft (22), stirring for 30-40min under the condition that the rotating speed is 800-, and (3) starting a third motor (51), driving a rotating shaft (52) to rotate by the third motor (51), driving a conical block (53) to rotate by the rotating shaft (52), driving a rotating plate (54) to rotate by the conical block (53), uniformly distributing the filtered substances on the sieve plate (5), blowing hot air out by a blowing head (16), drying the filtered substances at the temperature of 100-110 ℃, and collecting the dried substances by a discharge hole (12) to obtain the pure N-acetyl-D-glucosamine.
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| CN111821761A (en) * | 2020-08-06 | 2020-10-27 | 张雪燕 | Maltose thick liquid slag removal equipment |
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Application publication date: 20200710 |